CN219072986U - Multichannel equal-variable-distance pipetting device and gene processing equipment - Google Patents
Multichannel equal-variable-distance pipetting device and gene processing equipment Download PDFInfo
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Abstract
The application relates to the field of this application and provides a multichannel equidistant displacement pipetting device and gene processing equipment, multichannel equidistant displacement pipetting device includes: a main body frame; the guide rod is arranged on the main body frame; the plurality of variable-pitch guide blocks are movably arranged on the guide rod; the liquid transferring mechanism is connected with the variable-pitch guide block; the distance changing mechanism is arranged on the main body frame and connected with the distance changing guide block; the first driving mechanism is arranged on the main body frame, connected with the pipetting mechanism and used for driving the pipetting mechanism to lift; the second driving mechanism is arranged on the main body frame and connected with the distance changing mechanism and used for driving the distance changing mechanism to drive the distance changing guide block to slide on the guide rod so as to adjust the distance between the pipetting mechanisms. The multichannel equal-displacement pipetting device combines a cross connecting rod displacement structure with a double-output-shaft motor, and is dispersed or gathered from the middle to two sides when the displacement is carried out, the movement direction of a driving source and a displacement guide block are horizontally moved, the whole structure is not easy to be blocked when the displacement is carried out, the displacement precision is high, and the pipetting efficiency is improved.
Description
Technical Field
The application relates to the technical field of gene detection, in particular to a multichannel equal-variable-distance pipetting device and gene processing equipment.
Background
In the occasions of biopharmaceuticals, biochemical analysis, clinical examination and the like, different reagents are often required to be transferred to complete a mixed reaction, and the pipetting process is required to be repeated for a plurality of times. In early days, reagent pipetting was accomplished using manual pipettors, which were inefficient and highly affected by human factors. Later, in order to improve pipetting efficiency and accuracy, there have been developed on the market automatic pipetting devices with pipetting tips having separate channels and also multiple channels. However, the multichannel automatic pipetting device is preferred because it is only necessary to aspirate a reagent from a plurality of reagent containers simultaneously during one pipetting process and then move the reagent to another plurality of containers for reaction. However, in the conventional multi-channel automatic pipetting device, the intervals between the plurality of pipetting tips are generally fixed, and when the plurality of pipetting tips simultaneously withdraw liquid from the liquid withdrawal tube and move into the liquid charging container, the intervals between the liquid withdrawal containers must be the same as the intervals between the liquid charging containers. Under the condition that the distance between the liquid taking containers is different from the distance between the liquid adding containers, the multichannel fixed-distance automatic liquid transferring device cannot finish liquid transferring operation, so that the automatic liquid transferring device still has the defect of single function, and the application of the automatic liquid transferring device under different conditions is restricted.
In order to realize the equal distance changing function, a plurality of automatic liquid transferring devices capable of adjusting the distance between liquid taking containers are developed in the market, for example, patent application publication number CN 113694983A discloses a distance-variable liquid transferring device and a gun head distance-variable part, and the liquid transferring part of the structure mainly consists of a sample adding needle, and can realize the liquid transferring function only by connecting an external pump body through a hose; the distance separating plate and the guide post are in sliding friction in the distance changing process, and the distance separating groove on the distance separating plate is increased in clearance with the guide post due to friction after long-time use, so that the distance changing precision is poor. Another patent with application publication number CN 215843064U discloses a high-precision multi-head automatic displacement pipetting device, the displacement structure adopts a cross connecting rod mode, the displacement is asymmetric, one end is fixed, and the other end is moved; when the distance is changed, the innermost liquid transferring head does not move, the rest of the liquid transferring heads move outwards, and the asymmetric equal-distance changing outermost guide clamping plate is easy to clamp due to the fact that the distance changing stroke is long and the distance from a driving source is far. Secondly, the variable-pitch driving source is driven by a single side and is placed by the side, the occupied equipment space is large, and the variable-pitch driving source has only one but double-side driving capability and is placed in the middle, so that the stress is more balanced and the structure is more compact. The pipetting module has no plunger rod and is supplied with liquid by an external pump to the pipetting head. Therefore, the problem of low pipetting efficiency caused by poor precision of the displacement structure in the existing pipetting device still exists.
Disclosure of Invention
The utility model provides a multichannel equal displacement pipetting device and gene processing equipment, multichannel equal displacement pipetting device of this application adopts the form that cross connecting rod displacement structure and two go out the axle motor and combine together, and this structure is from centre to both sides dispersion or gathering together when the displacement, and the direction of motion of actuating source and displacement guide block are horizontal migration for the difficult card is dead when the displacement of whole structure, and displacement precision is higher, improves pipetting efficiency.
Embodiments of the present application are implemented as follows: in a first aspect, the present application provides a multichannel equidistant pipetting device comprising: a main body frame; the guide rod is arranged on the main body frame; the variable-pitch guide blocks are movably arranged on the guide rod; the liquid transferring mechanism is connected with the variable-pitch guide block; the distance changing mechanism is arranged on the main body frame and is connected with the distance changing guide block; the first driving mechanism is arranged on the main body frame, connected with the pipetting mechanism and used for driving the pipetting mechanism to lift; and the second driving mechanism is arranged on the main body frame, is connected with the distance changing mechanism and is used for driving the distance changing mechanism to drive the distance changing guide block to slide on the guide rod so as to adjust the distance between the pipetting mechanisms.
In the technical scheme, the distance-changing guide blocks are equidistant after being assembled, and the relative positions of the distance-changing guide blocks are calibrated without a special tool. The pipetting mechanism can realize pipetting and pipetting operations, and the distance-changing mechanism can adjust the distance between the pipetting mechanisms according to the position of the orifice plate of the consumable of the kit, so that the pipetting efficiency of the multichannel equal-distance-changing pipetting device is improved.
In one embodiment, the pitch mechanism comprises: at least one variable-pitch switching block connected with the second driving mechanism; and one end of the connecting rod assembly is connected with the variable-pitch adapter block, and the other end of the connecting rod assembly is connected with the variable-pitch guide block.
In the technical scheme, the equal-displacement structural design of connecting rod assembly switching is adopted, and the connecting mode of the cross connecting rod is simpler and easy to install.
In one embodiment, the variable-pitch adapter block is provided with an adapter connecting rod; the connecting rod assembly includes: the head end and the tail end of each connecting rod are movably connected with each other, each connecting rod is correspondingly connected with one variable-pitch guide block, and one connecting rod connected with the variable-pitch guide block is also connected with the switching connecting rod.
In the technical scheme, the multi-channel equal-displacement pipetting device adopts the mode that a cross connecting rod displacement structure is combined with a double-output-shaft motor, the structure is dispersed or gathered from the middle to two sides during displacement, and the movement direction of a driving source and a displacement block are both horizontally moved, so that the whole structure is not easy to be blocked during displacement, and the displacement precision is higher.
In one embodiment, the second driving mechanism includes: the driver body is provided with an output shaft; the output shaft is in transmission connection with the variable-pitch adapter block, and the driver body is used for driving the variable-pitch adapter block to slide on the guide rod and drive the connecting rod to pull up and shrink according to a preset direction.
In the technical scheme, in order to enable the stress in the pitch-changing process to be more uniform, the driver body can be arranged in the middle, the symmetrical equal pitch-changing and the stress are realized to be more uniform, the determined direction of the driver body is consistent with the pitch-changing direction, the force transmission loss is smaller, and the driving efficiency is higher. The driving position of the driver body is a variable-pitch guide block close to the middle position, and is not the innermost variable-pitch guide block and the outermost variable-pitch guide block, so that the stress of the driver body during outward and inward variable-pitch is closer, and the clamping of the innermost variable-pitch guide block and the outermost variable-pitch guide block during variable-pitch is effectively avoided.
In one embodiment, the pipetting mechanism comprises: a drive assembly and a pipette assembly; wherein the first driving mechanism is connected with the pipettor component through the transmission component; the pipette component is movably arranged on the transmission component.
In the technical scheme, the plurality of pipettor components are controlled by the first driving mechanism, so that each pipettor component is more compact, the cost is lower, and the function of driving the pipettor component to suck and inject liquid can be realized without an external pipeline or a pump body.
In an embodiment, the main body frame is provided with a sliding rail, and the transmission assembly includes: the plunger rod clamping plate is arranged on the sliding rail in a sliding manner; the first driving mechanism is connected with the plunger rod clamping plate, a chute is arranged on the plunger rod clamping plate, and a limit clamping block is arranged on the chute; the pipette assembly includes: the liquid transferring cavity is arranged on the variable-pitch guide block; the liquid transferring gun head is arranged at the bottom of the liquid transferring cavity; and one end of the plunger rod is limited in the chute through the limiting clamping block, and can slide along the chute, and the other end of the plunger rod can extend into the pipetting cavity.
In the technical scheme, the plunger rods on the liquid dispenser assemblies can freely slide in the sliding grooves along the distance-changing direction, and the first driving mechanism can drive the plunger rod clamping plate to move upwards or downwards along the sliding rails, so that the plunger rods are driven to move upwards or downwards, and the liquid sucking and injecting operation is completed in the liquid transferring cavity.
In one embodiment, the main body frame includes: a top plate, a bracket and a bottom plate; the first driving mechanism is arranged on the top plate; the sliding rail is arranged on the top plate; the top plate and the bottom plate are connected through the bracket; the second driving mechanism is arranged on the bracket; the guide rod is arranged on the bracket; the bottom plate is provided with a strip-shaped groove, and the pipette tip extends out of the bottom plate through the strip-shaped groove.
In the technical scheme, the structure of the main body frame is fixedly connected with each other by the top plate, the support and the bottom plate, and the structure of the whole main body frame is more stable, so that the multichannel equal-variable-distance pipetting device cannot sound and shake in the variable-distance operation process, and the pipetting effect is affected.
In an embodiment, the bottom plate is provided with a first through hole, and the multi-channel equal-variable-pitch pipetting device further includes: a suction head retracting mechanism; the suction head retracting mechanism comprises: the suction head guide block is arranged on the bracket and is provided with a second perforation; the elastic piece is arranged on the suction head guide block; the suction head withdrawing guide rod passes through the second perforation and extends out of the bottom plate through the first perforation, and the part of the suction head withdrawing guide rod which passes through the second perforation and extends out of the suction head withdrawing guide block is positioned in the elastic piece; and the suction head returning plate is connected with the bottom end of the suction head returning guide rod.
In the above technical scheme, when the pipette assembly finishes the operations of pipetting and pipetting, and the pipette needs to unload the pipette tips on the pipette tips, the plunger rod clamping plate is driven to move downwards through the first driving mechanism, when the plunger rod clamping plate moves downwards, the elastic element is compressed, and when the plunger rod clamping plate continues to move downwards to touch the pipette tip withdrawing guide rod, the pipette tip withdrawing guide rod moves downwards along the second perforation and the first perforation under the pushing action of the plunger rod clamping plate, so that the pipette tip withdrawing plate is driven to move downwards, and the pipette tips mounted on the pipette tips are unloaded through the acting force of the pipette tip withdrawing plate. After unloading is completed, the first driving mechanism drives the plunger rod clamping plate to move upwards, the elastic element restores the initial position under the elastic action force, the plunger rod clamping plate restores to the initial position, the plunger rod clamping plate leaves the suction head withdrawing guide rod, and the suction head withdrawing plate is restored.
In an embodiment, the pipetting cavity is further provided with an air pressure sensor pressing block, the air pressure sensor pressing block is provided with an air pressure sensor, the pipetting cavity is provided with a through hole, and the air pressure sensor can extend into the pipetting cavity through the through hole and is communicated with the pipetting cavity.
In the technical scheme, when the liquid sucking and injecting action is carried out on the liquid moving device component, the air pressure change in the liquid moving cavity is transmitted to the air pressure sensor. The air pressure sensor is used for detecting the liquid level. The air pressure sensors are arranged in two and are respectively arranged on the two pipetting cavities at the outermost side. By adopting the double-channel liquid level detection, the reliability and accuracy of liquid level detection can be improved.
In a second aspect, the present application provides a gene processing apparatus comprising a body and a multi-channel equal-variable-pitch pipetting device according to any one of the embodiments of the first aspect of the present application, the multi-channel equal-variable-pitch pipetting device being provided on the body.
In the technical scheme, the gene processing equipment completes liquid suction and liquid transfer operations through the multichannel and other variable-pitch liquid transfer devices, and can also carry out other nucleic acid detection operations such as cracking, washing, combining, eluting, PCR detection, centrifugal processing and the like, thereby improving the degree of automation of the gene processing equipment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered limiting the scope, and that other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of a multi-channel equal-displacement pipetting device according to an embodiment of the disclosure;
FIG. 2 is a front view of a multi-channel constant-displacement pipetting device according to one embodiment of the disclosure;
FIG. 3 is a first rear view of a multi-channel constant-displacement pipetting device according to one embodiment of the disclosure;
FIG. 4 is a second rear view of a multi-channel constant-displacement pipetting device according to one embodiment of the disclosure;
FIG. 5 is a diagram illustrating the connection of a pipette assembly to a variable-pitch guide block according to one embodiment of the present application;
FIG. 6 is a diagram illustrating the connection of a plunger rod to a plunger rod card in a pipette assembly according to one embodiment of the present disclosure;
FIG. 7 is a schematic view of an installation structure of a barometric sensor according to an embodiment of the present disclosure;
fig. 8 is a schematic structural diagram of a second driving mechanism according to an embodiment of the present application.
Icon:
1-a multichannel equal-variable-distance pipetting device; 100-a main body frame; 110-top plate; 120-bracket; 130-a bottom plate; 131-a bar-shaped groove; 132-first perforation; 140-slide rails; 200-guide rods; 300-a variable-pitch guide block; 310-circular through holes; 400-pipetting mechanism; 410-a transmission assembly; 411-plunger rod clip; 412-a chute; 413-limiting clamping blocks; 420-pipette assembly; 421-pipetting cavity; 4211-through holes; 422-pipette tip; 423-a plunger rod; 430-air pressure sensor briquetting; 440-barometric pressure sensor; 500-a pitch-changing mechanism; 510-a variable-pitch adapter block; 520-transfer link; 530-a linkage assembly; 531-connecting rod; 532—a locating hole; 533-locating pins; 600-a first drive mechanism; 700-a second drive mechanism; 710-a driver body; 720-an output shaft; 800-a suction head retracting mechanism; 810-a suction head guide block; 811-a second perforation; 820-elastic member; 830-a suction head guide bar; 840-suction head plate.
Detailed Description
The terms "first," "second," "third," and the like are used merely for distinguishing between descriptions and not for indicating a sequence number, nor are they to be construed as indicating or implying relative importance.
Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present application, it should be noted that, directions or positional relationships indicated by terms such as "inner", "outer", "left", "right", "upper", "lower", etc. are directions or positional relationships based on those shown in the drawings, or those that are conventionally put in use for the product of the application, are merely for convenience of description and simplification of the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be configured and operated in a specific direction, and therefore should not be construed as limiting the present application.
In the description of the present application, unless explicitly stated and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements.
The multichannel pipetting device can efficiently process liquid samples, and when the liquid samples are transferred among different consumables, the intervals of pipetting channels need to be changed correspondingly. In order to achieve the purpose of equal displacement, part of the multichannel pipetting devices adopt a cross connecting rod structure, but the displacement of the cross connecting rod structure is asymmetric, the problem of blockage easily exists during displacement, the whole stress of the multichannel pipetting devices is unbalanced due to single-side driving, and finally, the displacement precision is poor, so that pipetting efficiency is affected. Based on the above-mentioned problem, this application provides a multichannel isopiestic displacement pipetting device 1, adopts the form that cross connecting rod displacement structure and two play axle motors combined together, and this structure is from centre to both sides dispersion or gathering together when the displacement, and the direction of motion of driving source and displacement guide block are horizontal migration for the difficult card is dead when the displacement of whole structure, and displacement precision is higher.
Referring to fig. 1-4, a multi-channel constant-displacement pipetting device 1 includes: the device comprises a main body frame 100, a guide rod 200, a plurality of variable-pitch guide blocks 300, a pipetting mechanism 400, a variable-pitch mechanism 500, a first driving mechanism 600 and a second driving mechanism 700; wherein, guide arm 200 is located on main body frame 100, and a plurality of displacement guide blocks 300 activity are located on guide arm 200, and displacement mechanism 400 is connected with displacement guide blocks 300, and displacement mechanism 500 is located on main body frame 100, and is connected with displacement guide blocks 300, and first actuating mechanism 600 is located on main body frame 100, and is connected with displacement mechanism 400 for drive displacement mechanism 400 goes up and down, and second actuating mechanism 700 is located on main body frame 100, and is connected with displacement mechanism 500, is used for driving displacement mechanism 500 and drives displacement guide blocks 300 and slide on guide arm 200, in order to adjust the interval of displacement mechanism 400 each other.
In one embodiment, the main body frame 100 includes: top plate 110, bracket 120, and bottom plate 130; the first driving mechanism 600 is disposed on the top plate 110 and is located on the upper surface of the top plate 110, the top plate 110 and the bottom plate 130 are connected by the bracket 120, the second driving mechanism 700 is disposed on the bracket 120, and the guide rod 200 is disposed between the brackets 120. The connection between the top plate 110 and the bracket 120 and the bottom plate 130 and the bracket 120 may be fixed by bolts.
Referring to fig. 1, a pipetting mechanism 400 includes: the first driving mechanism 600 is connected with the pipette assembly 420 through the transmission assembly 410, and the pipette assembly 420 is movably arranged on the transmission assembly 410.
In an embodiment, the main body frame 100 is provided with a sliding rail 140, and in particular, the sliding rail 140 may be disposed on the lower surface of the top plate 110. The transmission assembly 410 includes: referring to fig. 6, a sliding groove 412 is formed in the plunger rod clamping plate 411, and a limit clamping block 413 is formed on the sliding groove 412.
Referring to fig. 5, the pipette assembly 420 includes: the liquid transferring cavity 421, the liquid transferring gun head 422 and the plunger rod 423, wherein the liquid transferring cavity 421 is arranged on the variable-pitch guide block 300 (the liquid transferring cavity 421 is arranged in front of the variable-pitch guide block 300 when seen from the direction shown in fig. 1), the liquid transferring gun head 422 is arranged at the bottom of the liquid transferring cavity 421 and used for loading a suction head, the suction head is used for taking liquid and transferring liquid, the bottom plate 130 is provided with the strip-shaped groove 131, the liquid transferring gun head 422 extends out of the bottom plate 130 through the strip-shaped groove 131, one end of the plunger rod 423 is limited in the chute 412 through the limiting clamping block 413 and can slide along the chute 412, and the other end of the plunger rod 423 can extend into the liquid transferring cavity 421.
Illustratively, the first driving mechanism 600 may be a motor, and an output end of the motor is connected through a screw transmission, so as to implement lifting control. In other embodiments, the first driving mechanism 600 may be a hydraulic cylinder or an air cylinder, wherein an output end of the hydraulic cylinder or the air cylinder is directly connected to the plunger rod clamping plate 411, and the hydraulic cylinder or the air cylinder drives the plunger rod clamping plate 411 to lift.
The first driving mechanism 600 can drive the plunger rod clamping plate 411 to move up or down along the sliding rail 140, so as to drive the plunger rod 423 to move up or down, and complete the sucking and injecting operation in the pipetting cavity 421. The plurality of the pipette assemblies 420 are controlled by adopting the first driving mechanism 600, so that each pipette assembly 420 is more compact and has lower cost, and the function of driving the pipette assembly 420 to suck and inject liquid can be realized without an external pipeline or a pump body.
It should be noted that, the number of the pipette assemblies 420 may be set according to the number of hole sites of consumable materials of the kit, for example, a single channel may be used, or multiple channels may be used, so that the efficiency of transferring liquid at one time may be improved. In this embodiment, the number of the pipette components 420 is eight, that is, the number of the pipetting chambers 421 is eight, and the multi-channel equal-variable-pitch pipetting device 1 is an eight-channel pipetting device. Accordingly, the variable-pitch guide block 300 is also set to eight.
In this embodiment, the first driving mechanism 600 is controlled to work, the first driving mechanism 600 drives the plunger rod clamping plate 411 to move downwards, the plunger rod clamping plate 411 moves to drive the plunger rod 423 connected in the chute 412 to move downwards, when reaching the position above the tube hole with the sample to be detected in the corresponding consumable of the kit, the suction head is controlled to extend into the tube hole of the consumable of the kit, the first driving mechanism 600 is controlled to work reversely, the plunger rod clamping plate 411 is driven to move upwards, the plunger rod 423 connected in the chute 412 is driven to move upwards by the movement of the plunger rod clamping plate 411, and the sample to be detected in the tube hole of the consumable of the kit is sucked into the liquid transferring cavity 421 through the suction head by utilizing the same principle as that of the syringe. Then, through controlling the whole transfer of displacement pipetting device 1 such as multichannel to be equipped with the unused tube hole top in the kit consumptive material, make the suction head stretch into the unused tube hole of kit consumptive material in, control first actuating mechanism 600 reverse work, first actuating mechanism 600 drive plunger rod cardboard 411 moves down, plunger rod cardboard 411's removal drives the plunger rod 423 of connecting in spout 412 and moves down, utilize the same principle with the syringe, pour into the unused tube hole of kit consumptive material into through the suction head with the sample that waits in the pipetting cavity 421, accomplish the imbibition finally, annotate the liquid operation.
Referring to fig. 7, a pressure sensor pressing block 430 is further disposed on the pipetting cavity 421, a pressure sensor 440 is disposed on the pressure sensor pressing block 430, a through hole 4211 is disposed on the pipetting cavity 421, and the pressure sensor 440 can extend into the pipetting cavity 421 through the through hole 4211 and communicate with the pipetting cavity 421. When the pipette assembly 420 has the sucking and injecting action, the air pressure change in the pipetting cavity 421 is transferred to the air pressure sensor 440. The principle of the air pressure sensor 440 for detecting the liquid level is as follows: when the liquid level detection is started by the pipette assembly 420, the plunger rod clamping plate 411 is driven to move downwards along with the first driving mechanism 600, so as to drive the plunger rod 423 to move downwards, and the detection process can be divided into two stages. Before contacting the liquid level, the interior of the pipetting cavity 421 is communicated with the atmospheric pressure, and no obvious air pressure fluctuation exists in the pipetting cavity; after contacting the liquid surface, the liquid seals the tip of the suction head, and as the depth of the immersed liquid surface increases, the liquid begins to enter the suction head through the tip of the suction head under the action of pressure, the air pressure in the liquid transferring cavity 421 increases, and when the increasing amplitude exceeds a set threshold value, a detection signal is triggered. In this embodiment, the air pressure sensors 440 may be provided in two and disposed on the two pipetting chambers 421 located at the outermost sides, respectively. The dual-channel liquid level detection can improve the reliability and accuracy of liquid level detection.
Since the pipette gun assembly 420 is required to replace a new pipette tip for other mixing operations after pipetting and pipetting, the pipette tip withdrawal mechanism 800 is further provided in the multi-channel or other variable-pitch pipetting device 1 of the present application. Referring to fig. 1, a first through hole 132 is formed in the bottom plate 130. The suction head retracting mechanism 800 includes: the suction head guide 810, the elastic member 820, the suction head guide 830, and the suction head plate 840. The suction head guide block 810 is disposed on the bracket 120 and fixed, a second perforation 811 is disposed on the suction head guide block 810, a bottom end of the elastic member 820 is disposed on the suction head guide block 810, the suction head guide rod 830 passes through the suction head guide block 810 and is a second perforation 811, and extends out of the bottom plate 130 through the first perforation 132 on the bottom plate 130, a portion of the suction head guide rod 830 passing through the second perforation 811 and extending out of the suction head guide block 810 is disposed in the elastic member 820, the suction head plate 840 is connected with the bottom end of the suction head guide rod 830, and a middle portion of the suction head plate 840 may be grooved, so that a bottom portion of the pipette tip 422 extends out of the suction head plate 840. Illustratively, the elastic member 820 may be a spring.
In this embodiment, when the pipette assembly 420 is required to unload the pipette tips on the pipette tips 422 after the pipetting and pipetting operations are completed, the plunger rod clamping plate 411 is driven to move downward by the first driving mechanism 600, the elastic member 820 is compressed during the downward movement of the plunger rod clamping plate 411, and when the plunger rod clamping plate 411 continues to move downward to touch the pipette tip withdrawing guide rod 830, the pipette tip withdrawing guide rod 830 moves downward along the second perforation 811 and the first perforation 132 under the pushing action of the plunger rod clamping plate 411, so as to drive the pipette tip withdrawing plate 840 to move downward, and the pipette tips mounted on the pipette tips 422 are unloaded by the acting force of the pipette tip withdrawing plate 840. After the unloading is completed, the first driving mechanism 600 drives the plunger rod clamping plate 411 to move upwards, the elastic element 820 returns to the initial position under the elastic force, the plunger rod clamping plate 411 returns to the initial position, the plunger rod clamping plate 411 leaves the suck-back head guide rod 830, and the suck-back head plate 840 returns.
Referring to fig. 3 and 4, the pitch mechanism 500 includes: at least one pitch horn 510 and a link assembly 530, the pitch horn 510 being connected to the second driving mechanism 700, one end of the link assembly 530 being connected to the pitch horn 510, and the other end being connected to the pitch guide block 300 (the link assembly 530 being provided on the back of the pitch guide block 300 as seen in the direction shown in fig. 4).
In an embodiment, the pitch-changing adapter block 510 is provided with an adapter link 520, the link assembly 530 comprises a plurality of links 531, the head end and the tail end of each link 531 are movably connected with each other, each link 531 is correspondingly connected with a pitch-changing guide block 300, and one link 531 connected with the pitch-changing guide block 300 is also connected with the adapter link 520.
In one embodiment, the second driving mechanism 700 may specifically include: the driver body 710, the driver body 710 is provided with an output shaft 720, the output shaft 720 is in transmission connection with the variable-pitch adapter block 510, and the driver body 710 is used for driving the variable-pitch adapter block 510 to slide on the guide rod 200, so as to drive the connecting rod 531 to pull up and retract according to a preset direction.
For example, referring to fig. 8, the second driving mechanism 700 is a dual-shaft motor, two ends of the driver body 710 are provided with output shafts 720, the output shafts 720 are provided with screw structures, and the screw structures on the two output shafts 720 are opposite, for example, the screw structure on the left output shaft 720 is a left-handed screw, and the screw structure on the right output shaft 720 is a right-handed screw. The number of the variable-pitch switching blocks 510 is correspondingly two, and the variable-pitch switching blocks are respectively in threaded connection with the output shaft 720 through bearing blocks. The adapter links 520 on each of the variable pitch adapter blocks 510 have one end connected to the link 531 (e.g., the side of the adapter link 520 adjacent to the actuator body 710 shown in fig. 4 is connected to the link 531) and the other end not connected to the link 531, otherwise the link 531 cannot be pulled up or retracted.
The head end and the tail end of each connecting rod 531 may be movably connected by a pin, and one end of each connecting rod 531 may be connected with the variable-pitch guide block 300 by a pin. Illustratively, each link 531 has a positioning hole 532 provided at a head end and a tail end, the head end and the tail end of the link 531 are locked by a positioning pin 533, and the middle of each link 531 is locked to the variable-pitch guide block 300 by the positioning pin 533, respectively. And the lengths of the two links 531 at the outermost ends of the link assembly 530 are only half of the lengths of the other links 531, so that the ends of the two links 531 at the outermost ends of the link assembly 530 can be locked on the rear surface of the variable-pitch guide block 300 directly by the positioning pins 533.
When the driver body 710 drives the two output shafts 720 to rotate, the pitch-changing adapter blocks 510 located at the left side of the driver body 710 move leftwards (in the direction indicated by the arrow in fig. 8), the pitch-changing adapter blocks 510 located at the right side of the driver body 710 move rightwards, and the two pitch-changing adapter blocks 510 move away from the driver body 710 (i.e. move back to back), so as to drive the two pitch-changing adapter blocks 510 to pull the connecting rod 531 from the center to lift from the two ends, the lifting of the connecting rod 531 drives each pitch-changing guide block 300 connected with the connecting rod 531 to slide on the guide rod 200 in the direction of the two ends of the guide rod 200, thereby changing the positions of the pitch-changing guide blocks 300 on the guide rod 200, enabling the distance between the adjacent pitch-changing guide blocks 300 to be correspondingly adjusted along with the distance between the orifice plates on the consumable cartridge, and simultaneously, the distance 423 in the liquid-transferring cavity 421 connected with the pitch-changing guide blocks 300 can slide from the center to the two ends in the chute 412 of the plunger rod 411, thereby achieving the purpose of adjusting the distance between the adjacent liquid-transferring cavities 421, and the purpose of the liquid-transferring assembly 420.
If the distance between the pipette assemblies 420 needs to be adjusted according to the distance between the hole plates on the consumable materials of the kit, the driver body 710 can be controlled to drive the two output shafts 720 to reversely rotate in the opposite directions, so that the two displacement transfer blocks 510 respectively move in opposite directions, and further the transfer connecting rods 520 on the two displacement transfer blocks 510 are driven to pull the connecting rods 531 to shrink from two ends to the center, the lifting of the connecting rods 531 drives each displacement guide block 300 connected with the connecting rods 531 to slide on the guide rods 200 towards the center of the guide rods 200, so that the positions of the displacement guide blocks 300 on the guide rods 200 are changed, the distance between the adjacent displacement guide blocks 300 is correspondingly adjusted along with the distance between the hole plates on the consumable materials of the kit, and meanwhile, the plunger rods 423 in the displacement cavities 421 connected with the displacement guide blocks 300 can slide in the slide from two ends to the center in the slide grooves 412 of the clamping plates 411, so that the distance between the adjacent displacement cavities 421 is adjusted, and the purpose of the pipette assemblies 420 is achieved.
In the above embodiment, the plunger rod 423 on each pipette assembly 420 can freely slide in the sliding groove 412 along the pitch direction, and the equal pitch structure design of the cross connecting rod is adopted, so that the connection mode of the cross connecting rod is simpler and the installation is easy. The assembly of the variable-pitch guide blocks 300 is equidistant, and a special tool is not needed to calibrate the relative positions of the variable-pitch guide blocks 300.
In order to improve the stability of the variable-pitch guide block 300 when sliding on the guide rods 200 and avoid shaking, in an embodiment, referring to fig. 3, two guide rods 200 are respectively disposed between the brackets 120 and are respectively disposed at the top end and the bottom end of the brackets 120, two circular through holes 310 are respectively disposed at two ends of the variable-pitch guide block 300, the top of the guide rod 200 disposed at the top of the brackets 120 passes through the circular through hole 310 at the top end of the variable-pitch guide block 300, and the top of the guide rod 200 disposed at the bottom end of the brackets 120 passes through the circular through hole 310 at the bottom end of the variable-pitch guide block 300, so that the variable-pitch guide block 300 is slidably sleeved between the two guide rods 200.
In addition, in order to make the stress more even in the range-changing process, the driver body 710 can be arranged in the middle, so that symmetrical equal range-changing and more even stress are realized, the determined direction of the driver body 710 is consistent with the range-changing direction, the force transmission loss is smaller, and the driving efficiency is higher. The driving position of the driver body 710 is the displacement guide block 300 close to the middle position, and is not the innermost and the outermost displacement guide blocks 300, so that the stress during the outward and inward displacement is closer, and the clamping of the innermost and the outermost displacement guide blocks 300 during the displacement is effectively avoided.
The application also provides gene processing equipment, which comprises a body and the multichannel equal-variable-distance pipetting device 1, wherein the multichannel equal-variable-distance pipetting device 1 is arranged on the body. The gene processing equipment can complete liquid suction and liquid displacement operation through the multichannel equal-variable-distance liquid displacement device 1, and can also carry out other nucleic acid detection operations such as cracking, washing, combining, eluting, PCR detection, centrifugal processing and the like. The other nucleic acid detection procedures such as cleavage, washing, binding, elution, PCR detection, centrifugation and the like are not described herein.
The working principle of the multichannel equal-variable-distance pipetting device 1 is specifically as follows: according to the distance between the perforated plates on the consumable parts of the reagent box, firstly, the driver body 710 is controlled to drive the two output shafts 720 to rotate, so that the distance-changing turning blocks 510 positioned on the left side of the driver body 710 move leftwards (the direction indicated by the arrow in fig. 8), the distance-changing turning blocks 510 positioned on the right side of the driver body 710 move rightwards, the two distance-changing turning blocks 510 move away from the driver body 710 (namely move backwards), the turning connecting rods 520 on the two distance-changing turning blocks 510 are driven to pull the connecting rods 531 from the center to the two ends, the lifting of the connecting rods 531 drives each distance-changing turning block 300 connected with the connecting rods 531 to slide on the guide rods 200 in the direction of the two ends of the guide rods 200, the positions of the distance-changing turning blocks 300 on the guide rods 200 are changed, the distance between the adjacent distance-changing turning blocks 300 is correspondingly adjusted along with the distance between the perforated plates on the consumable parts of the reagent box, and meanwhile, the 423 in the liquid-moving cavity 421 connected with the distance-changing guide blocks 300 can slide from the center to the two ends in the sliding grooves 412 of the plunger rod 411 of the plunger rod, so that the distance between the adjacent liquid-changing cavities 421 can be adjusted by the distance between the adjacent liquid-changing cavities 420.
When the distance between adjacent pipette assemblies 420 is adjusted, the first driving mechanism 600 is controlled to work, the first driving mechanism 600 drives the plunger rod clamping plate 411 to move downwards, the movement of the plunger rod clamping plate 411 drives the plunger rod 423 connected in the sliding groove 412 to move downwards, when the position above the tube hole with the sample to be detected in the corresponding reagent kit consumable is reached, the suction head is controlled to extend into the tube hole of the reagent kit consumable, the first driving mechanism 600 is controlled to work reversely, the plunger rod clamping plate 411 is driven to move upwards, the movement of the plunger rod clamping plate 411 drives the plunger rod 423 connected in the sliding groove 412 to move upwards, and the sample to be detected in the tube hole of the reagent kit consumable is sucked into the pipetting cavity 421 through the suction head by utilizing the same principle as that of the syringe. Then, through controlling the whole transfer of displacement pipetting device 1 such as multichannel to be equipped with the unused tube hole top in the kit consumptive material, make the suction head stretch into the unused tube hole of kit consumptive material in, control first actuating mechanism 600 reverse work, first actuating mechanism 600 drive plunger rod cardboard 411 moves down, plunger rod cardboard 411's removal drives the plunger rod 423 of connecting in spout 412 and moves down, utilize the same principle with the syringe, pour into the unused tube hole of kit consumptive material into through the suction head with the sample that waits in the pipetting cavity 421, accomplish the imbibition finally, annotate the liquid operation.
It should be noted that, in this embodiment, the station adjusting device is provided in the gene processing apparatus, and the station adjusting device includes a horizontal position adjusting device and a vertical position adjusting device, and is used for adjusting the moving position of the multi-channel equal-variable-distance pipetting device 1, and by using the horizontal position adjusting device, the multi-channel equal-variable-distance pipetting device 1 can horizontally move to the corresponding position on the reagent kit consumable, and at the same time, the vertical height of the multi-channel equal-variable-distance pipetting device 1 is adjusted by using the vertical position adjusting device, so that the height of the multi-channel equal-variable-distance pipetting device 1 can be adjusted according to the pipe hole position on the reagent kit consumable. The specific structure of the above-mentioned station adjusting apparatus will not be described in detail.
It should be noted that, without conflict, features in the embodiments of the present application may be combined with each other.
The foregoing is merely a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and variations may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.
Claims (10)
1. A multi-channel constant-pitch pipetting device comprising:
a main body frame;
the guide rod is arranged on the main body frame;
the variable-pitch guide blocks are movably arranged on the guide rod;
the liquid transferring mechanism is connected with the variable-pitch guide block;
the distance changing mechanism is arranged on the main body frame and is connected with the distance changing guide block;
the first driving mechanism is arranged on the main body frame, connected with the pipetting mechanism and used for driving the pipetting mechanism to lift; and
the second driving mechanism is arranged on the main body frame, is connected with the distance changing mechanism and is used for driving the distance changing mechanism to drive the distance changing guide block to slide on the guide rod so as to adjust the distance between the pipetting mechanisms.
2. The multi-channel equal-range pipetting device of claim 1 wherein the range mechanism comprises:
at least one variable-pitch switching block connected with the second driving mechanism;
and one end of the connecting rod assembly is connected with the variable-pitch adapter block, and the other end of the connecting rod assembly is connected with the variable-pitch guide block.
3. The multi-channel equal-displacement pipetting device of claim 2 wherein the displacement adapter block is provided with an adapter link;
the connecting rod assembly includes: the head end and the tail end of each connecting rod are movably connected with each other, each connecting rod is correspondingly connected with one variable-pitch guide block, and one connecting rod connected with the variable-pitch guide block is also connected with the switching connecting rod.
4. A multi-channel alike variable-pitch pipetting device as recited in claim 3 wherein the second drive mechanism comprises:
the driver body is provided with an output shaft;
the output shaft is in transmission connection with the variable-pitch adapter block, and the driver body is used for driving the variable-pitch adapter block to slide on the guide rod and drive the connecting rod to pull up and shrink according to a preset direction.
5. The multi-channel constant pitch pipetting device of claim 1 wherein the pipetting mechanism comprises: a drive assembly and a pipette assembly; wherein,,
the first driving mechanism is connected with the pipettor assembly through the transmission assembly;
the pipette component is movably arranged on the transmission component.
6. The multi-channel constant pitch pipetting device of claim 5 wherein the body frame is provided with a slide rail and the drive assembly comprises: the plunger rod clamping plate is arranged on the sliding rail in a sliding manner; the first driving mechanism is connected with the plunger rod clamping plate, a chute is arranged on the plunger rod clamping plate, and a limit clamping block is arranged on the chute;
the pipette assembly includes: the liquid transferring cavity is arranged on the variable-pitch guide block; the liquid transferring gun head is arranged at the bottom of the liquid transferring cavity; and one end of the plunger rod is limited in the chute through the limiting clamping block, and can slide along the chute, and the other end of the plunger rod can extend into the pipetting cavity.
7. The multi-channel constant pitch pipetting device of claim 6 wherein the body frame comprises: a top plate, a bracket and a bottom plate;
the first driving mechanism is arranged on the top plate;
the sliding rail is arranged on the top plate;
the top plate and the bottom plate are connected through the bracket;
the second driving mechanism is arranged on the bracket;
the guide rod is arranged on the bracket;
the bottom plate is provided with a strip-shaped groove, and the pipette tip extends out of the bottom plate through the strip-shaped groove.
8. The multi-channel constant pitch pipetting device of claim 7 wherein the bottom plate is provided with a first perforation, the multi-channel constant pitch pipetting device further comprising: a suction head retracting mechanism; the suction head retracting mechanism comprises:
the suction head guide block is arranged on the bracket and is provided with a second perforation;
the elastic piece is arranged on the suction head guide block;
the suction head withdrawing guide rod passes through the second perforation and extends out of the bottom plate through the first perforation, and the part of the suction head withdrawing guide rod which passes through the second perforation and extends out of the suction head withdrawing guide block is positioned in the elastic piece; and
the suction head returning plate is connected with the bottom end of the suction head returning guide rod.
9. The multi-channel equal-displacement pipetting device according to claim 6, wherein the pipetting cavity is further provided with a barometric sensor pressing block, the barometric sensor pressing block is provided with a barometric sensor, the pipetting cavity is provided with a through hole, and the barometric sensor can extend into the pipetting cavity through the through hole and is communicated with the pipetting cavity.
10. A gene processing apparatus comprising a body and a multi-channel isoparaffinity pipetting device as recited in any one of claims 1 to 9, the multi-channel isoparaffinity pipetting device being disposed on the body.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202223604352.7U CN219072986U (en) | 2022-12-27 | 2022-12-27 | Multichannel equal-variable-distance pipetting device and gene processing equipment |
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| CN202223604352.7U CN219072986U (en) | 2022-12-27 | 2022-12-27 | Multichannel equal-variable-distance pipetting device and gene processing equipment |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116496881A (en) * | 2023-06-26 | 2023-07-28 | 杭州奥盛仪器有限公司 | Full-automatic gene processing equipment and method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116496881A (en) * | 2023-06-26 | 2023-07-28 | 杭州奥盛仪器有限公司 | Full-automatic gene processing equipment and method |
| CN116496881B (en) * | 2023-06-26 | 2023-09-29 | 杭州奥盛仪器有限公司 | Full-automatic gene processing method |
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