CN219070271U - Living calcium imaging and behavior monitoring integrated system - Google Patents
Living calcium imaging and behavior monitoring integrated system Download PDFInfo
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- CN219070271U CN219070271U CN202222565792.XU CN202222565792U CN219070271U CN 219070271 U CN219070271 U CN 219070271U CN 202222565792 U CN202222565792 U CN 202222565792U CN 219070271 U CN219070271 U CN 219070271U
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Abstract
The utility model relates to a living body calcium formation of image and behavior control integrated system, including the action module of making a video recording, the brain fluorescence is shot the module, first judgement module and receiving module, brain fluorescence is shot the module and is set up in the brain of living body, brain fluorescence is shot the module and is used for shooing the brain fluorescence image of living body, the action is shot the module and is used for shooing the action image of living body, action is shot the module and is all with first judgement module communication connection, first judgement module is used for judging whether action image and brain fluorescence image are synchronous, first judgement module and receiving module communication connection, under the circumstances that action image and brain fluorescence image are synchronous, action image and brain fluorescence image in the presupposed time quantum are stored to receiving module. The scheme can solve the problem that the synchronous record of each calcium signal change of cerebral cortex neurons and the behavior matched with each calcium signal change of cerebral cortex neurons in the related technology is not involved, so that the research of other behaviors of workers on biology and interaction of brains cannot be satisfied.
Description
Technical Field
The utility model relates to the technical field of camera shooting, in particular to an integrated system for living calcium imaging and behavior monitoring.
Background
Brain science is an important research direction of international leading-edge attention at present, wherein, research on direct interaction of brain with the outside world is also one of brain science research. Neurons in the brain are interconnected by collective behavior to form a variety of neural loops to perform different functions. The research direction of the nerve loops is to search the nerve cell forming each nerve loop, and research on how the nerve cells generate communication interaction to form specific loops and vital activities of the loops in the central nervous system, and many researches prove that in the process of analyzing the control mechanism of the nerve loops under physiological or pathological conditions, the activity of the nerve cells in brain tissues can be accurately reflected by monitoring the change of calcium ions of important biological sensors in the nerve cells in real time, but how to record the vital activities and the change of calcium ions is a difficult problem.
In order to solve the above problems, in the related art, the utility model of China with the patent number 201910008322.3 provides a method for constructing a brain-computer interface behavioral model, which comprises the following steps: operational behavior phase: training a mouse to obtain rewards through operation behaviors in T1 after prompt occurrence, wherein the number of times of successfully obtaining the operation behaviors of rewards is N, simultaneously, respectively recording the calcium signal F1 value of the mouse M1 cortical neuron when the N times of operation behaviors occur, marking the calcium signal of the mouse M1 cortical neuron as F0 when the prompt occurrence occurs, calculating the average value of the change rate (F1-F0)/F0 of the calcium signal, and setting the average value as a threshold value; idea control stage: training a mouse, controlling the calcium signal of the mouse M1 cortical neuron in the T2 after the prompt appears through ideas, recording the F2 value of the calcium signal of the mouse M1 cortical neuron in the T2, obtaining rewards by the mouse after the change rate (F2-F0)/F0 of the calcium signal exceeds the threshold value, recording as a successful test, counting the success rate of the mouse in the daily test, recording the total time required for completing the M successful tests every day, and accordingly obtaining rewards by the training mouse through specific behaviors in different time periods, and completing the construction of the brain-computer interface behavioral model.
The method only takes the success rate in each test and the total time required for completing M successful tests as evaluation indexes, and does not relate to the synchronous record of each calcium signal change of cerebral cortex neurons and the behavior matched with each calcium signal change, so that the method cannot meet the research of interaction of other behaviors of workers on organisms and brains.
Disclosure of Invention
Based on the method, an integrated system for living calcium imaging and behavior monitoring is provided, so that the problem that the research on interaction between other behaviors of a worker on living things and the brain cannot be met because synchronous recording of each calcium signal change of cerebral cortex neurons and behaviors matched with each calcium signal change in the related art is solved.
The utility model provides an integrated system is imaged to living body calcium and behavior control, includes action camera module, brain fluorescence shooting module, first judgement module and receiving module, brain fluorescence shooting module sets up in the brain of living body, brain fluorescence shooting module is used for taking the brain fluorescence image of living body, action camera module is used for taking the action image of living body, action camera module with brain fluorescence shooting module all with first judgement module communication connection, first judgement module is used for judging whether action image with brain fluorescence image is synchronous, first judgement module with receiving module communication connection the action image with under the synchronous condition of brain fluorescence image, receiving module stores action image in the preset time quantum with brain fluorescence image.
Preferably, the integrated system for living calcium imaging and behavior monitoring further comprises a calibration module, wherein the behavior camera module and the brain fluorescence camera module are both in communication connection with the calibration module, and the calibration module calibrates the acquisition time of the behavior image and the brain fluorescence image under the condition that the behavior image and the brain fluorescence image are not synchronous.
Preferably, the above-mentioned living body calcium formation of image and behavior monitoring integrated system, the brain fluorescence shooting module includes first acquisition module, second judgement module, first control module and shooting module, first acquisition module with second judgement module communication connection, second judgement module with first control module communication connection, shooting module is used for shooting brain fluorescence image, shooting module with first control module communication connection, first acquisition module is used for acquireing to the actual intensity of the thorn laser of living body, second judgement module is used for judging whether actual intensity is the same with predetermineeing intensity, actual intensity with predetermineeing under the condition that intensity is different, first control module control actual intensity is the same with predetermineeing intensity.
Preferably, the above-mentioned living body calcium imaging and behavior monitoring integrated system, the brain fluorescence shooting module includes second acquisition module, third judgement module, second control module and shooting module, the second acquisition module with third judgement module communication connection, third judgement module with second control module communication connection, shooting module is used for shooting brain fluorescence image, shooting module with second control module communication connection, the second acquisition module is used for obtaining the actual diopter of shooing the living body, third judgement module is used for judging whether actual diopter is the same with predetermineeing diopter, in actual diopter with predetermineeing under the circumstances that diopter is different, the second control module control actual diopter is the same with predetermineeing diopter.
The technical scheme that this application adopted can reach following beneficial effect:
in the living body calcium imaging and behavior monitoring integrated system disclosed by the application, including behavior camera module, brain fluorescence shooting module, first judgement module and receiving module, brain fluorescence shooting module sets up in the brain of living body, and brain fluorescence shooting module is used for shooing the brain fluorescence image of living body, and behavior camera module is used for shooing the behavior image of living body, and first judgement module is used for judging whether behavior image and brain fluorescence image are synchronous, and under the synchronous condition of behavior image and brain fluorescence image, the receiving module stores behavior image and brain fluorescence image in the preset time quantum. According to the structure, the behavior image and the brain fluorescence image of the living body are shot through the behavior shooting module and the brain fluorescence shooting module respectively, and then the receiving module can store the behavior image and the brain fluorescence image under the condition that the behavior image and the brain fluorescence image are synchronous, so that the research of synchronous interaction of the behavior of a worker on the living body and the brain can be met.
Drawings
FIG. 1 is a schematic diagram of an integrated system for in-vivo calcium imaging and behavior monitoring as disclosed in embodiments of the present application;
FIG. 2 is a schematic diagram of a brain fluorescence imaging module according to an embodiment of the present disclosure;
FIG. 3 is another schematic diagram of a brain fluorescence imaging module according to an embodiment of the present disclosure;
FIG. 4 is an energy distribution diagram of a light source;
FIG. 5 is a desired energy distribution diagram of light emitted by a light source;
FIG. 6 is an exploded view of a camera module disclosed in an embodiment of the present application;
FIG. 7 is a front view of a camera module disclosed in an embodiment of the present application;
FIG. 8 is a cross-sectional view of FIG. 6;
fig. 9 is an exploded cross-sectional view of a housing as disclosed in an embodiment of the present application.
Wherein: the system comprises a behavior shooting module 100, a brain fluorescence shooting module 200, a first acquisition module 210, a second judgment module 220, a first control module 230, a shooting module 240, a lens 241, a light inlet end 241a, a light outlet end 241b, a blue light filter 241c, a shell 242, an inner cavity 242a, a first opening 242b, a second opening 242c, a third opening 242d, a spectroscope 243, an image receiver 244, an excitation light source 245, a self-focusing lens 246, an achromatic lens 247, a focusing lens 248, a liquid lens 249, a second acquisition module 250, a third judgment module 260, a second control module 270, a first judgment module 300, a receiving module 400 and a calibration module 500.
Detailed Description
In order that the utility model may be readily understood, a more complete description of the utility model will be rendered by reference to the appended drawings. The drawings illustrate preferred embodiments of the utility model. This utility model may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," "top," "bottom," "top," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 to 3, an integrated system for living calcium imaging and behavior monitoring is disclosed in the embodiments of the present application, and the disclosed integrated system for living calcium imaging and behavior monitoring includes a behavior camera module 100, a brain fluorescence camera module 200, a first judgment module 300 and a receiving module 400.
Specifically, the brain fluorescence shooting module 200 is disposed on the brain of the living body, the brain fluorescence shooting module 200 is used for shooting brain fluorescence images of the living body, the behavior shooting module 100 is used for shooting behavior images of the living body, the behavior shooting module 100 and the brain fluorescence shooting module 200 are both in communication connection with the first judging module 300, the first judging module 300 is used for judging whether the behavior images are synchronous with the brain fluorescence images, the first judging module 300 is in communication connection with the receiving module 400, and the receiving module 400 stores the behavior images and the brain fluorescence images in a preset time period under the condition that the behavior images are synchronous with the brain fluorescence images.
In the use process of the integrated system for living calcium imaging and behavior monitoring, the brain fluorescence shooting module 200 can shoot brain fluorescence images, the behavior shooting module 100 can shoot behavior images, the first judging module 300 can judge whether the behavior images are synchronous with the brain fluorescence images, and the receiving module 400 can store the behavior images and the brain fluorescence images in a preset time period under the condition that the behavior images are synchronous with the brain fluorescence images, so that the research of synchronous interaction of the behaviors of workers and the brain is facilitated.
In the living body calcium imaging and behavior monitoring integrated system disclosed by the application, including behavior camera module 100, brain fluorescence shooting module 200, first judgement module 300 and receiving module 400, brain fluorescence shooting module 200 sets up in the brain of living body, brain fluorescence shooting module 200 is used for shooting the brain fluorescence image of living body, behavior camera module 100 is used for shooting the behavior image of living body, behavior camera module 100 and brain fluorescence shooting module 200 all with first judgement module 300 communication connection, first judgement module 300 is used for judging whether behavior image and brain fluorescence image are synchronous, first judgement module 300 and receiving module 400 communication connection, under the circumstances that behavior image and brain fluorescence image are synchronous, behavior image and brain fluorescence image in the presupposed time period are stored to receiving module 400. The above structure respectively shoots the behavioral image and the brain fluorescence image of the living body through the behavioral image shooting module 100 and the brain fluorescence shooting module 200, and then the receiving module 400 can store the behavioral image and the brain fluorescence image under the condition that the behavioral image and the brain fluorescence image are synchronous, thereby being capable of meeting the research of the synchronous interaction of the biological behaviors and the brain by the staff.
In this embodiment of the present application, the integrated system for calcium imaging and behavior monitoring of living body may further include a calibration module 500, specifically, the behavior imaging module 100 and the brain fluorescence imaging module 200 may be both connected to the calibration module 500 in a communication manner, and in a case that the behavior image and the brain fluorescence image are not synchronous, the calibration module 500 may calibrate the acquisition time of the behavior image and the brain fluorescence image.
In the above structure, when the behavioral image and the brain fluoroscopic image are not synchronized, the acquisition time of the behavioral image and the brain fluoroscopic image is calibrated by the calibration module 500, thereby avoiding the problem that the behavioral image and the brain fluoroscopic image are continuously out of synchronization.
In addition, the synchronization is achieved by calibrating the acquisition time of the behavioral image and the acquisition time of the brain fluorescence image by the calibration module 500, so that the behavioral image and the brain fluorescence image can be stored by the receiving module 400, thereby avoiding the captured behavioral image and brain fluorescence image from being discarded.
In this embodiment of the present application, the brain fluorescence shooting module 200 includes a first obtaining module 210, a second judging module 220, a first control module 230 and a shooting module 240, specifically, the first obtaining module 210 may be in communication connection with the second judging module 220, the second judging module 220 may be in communication connection with the first control module 230, the shooting module 240 is used for shooting a brain fluorescence image, the shooting module 240 may be in communication connection with the first control module 230, the first obtaining module 210 may be used for obtaining an actual intensity of a laser beam to a living body, the second judging module 220 may be used for judging whether the actual intensity is the same as a preset intensity, and the first control module 230 may control the actual intensity to be the same as the preset intensity under the condition that the actual intensity is different from the preset intensity.
In the above structure, since the GCaMP virus of the brain of the living body emits green fluorescence through the stimulation of the stimulating light, the intensity of the stimulating light is in direct proportion to the intensity of the green fluorescence, however, the photographing effect of the brain fluorescence image is in direct proportion to the intensity of the green fluorescence, and the preset intensity is verified by a plurality of experiments by a worker, the photographing effect of the brain fluorescence image is better under the preset intensity, and under the condition that the actual intensity is different from the preset intensity, the first control module 230 controls the actual intensity to be the same as the preset intensity, thereby ensuring the photographing effect of the brain fluorescence image.
In this embodiment of the present application, the brain fluorescence shooting module 200 may include a second obtaining module 250, a third judging module 260, a second control module 270, and a shooting module 240, specifically, the second obtaining module 250 is in communication connection with the third judging module 260, the third judging module 260 is in communication connection with the second control module 270, the shooting module 240 is used for shooting a brain fluorescence image, the shooting module 240 is in communication connection with the second control module 270, the second obtaining module 250 may be used for obtaining an actual diopter shot by a living body, the third judging module 260 may be used for judging whether the actual diopter is the same as a preset diopter, and the second control module 270 may control the actual diopter to be the same as the preset diopter under the condition that the actual diopter is different from the preset diopter.
In the above structure, since the diopter can affect the focusing accuracy of shooting brain fluorescence images, and the preset diopter is verified by a plurality of experiments by a worker, the brain fluorescence image with higher definition can be shot under the preset diopter, and under the condition that the actual diopter is different from the preset diopter, the second control module 270 controls the actual diopter to be the same as the preset diopter, so that the focusing accuracy of shooting brain fluorescence image is ensured.
As shown in fig. 6 to 9, the photographing module may include a lens 241, a housing 242, a beam splitter 243, an image receiver 244, an excitation light source 245, and a self-focusing lens 246.
The housing 242 can provide a mounting base for the lens 241, the beam splitter 243, the image receiver 244, the excitation light source 245 and the self-focusing lens 246, the excitation light source 245 can emit a stimulating light, the stimulating light can stimulate the GCaMP virus of the brain of a living body to make it emit green fluorescence, and the self-focusing lens 246 has focusing and imaging functions.
Specifically, the housing 242 may be provided with an inner cavity 242a, the first opening 242b, the second opening 242c and the third opening 242d may be in communication with the inner cavity 242a, the first opening 242b may be opposite to the second opening 242c, the image receiver 244, the excitation light source 245 and the self-focusing lens 246 may be disposed on the housing 242, the receiving surface of the image receiver 244 may be opposite to the first opening 242b, the self-focusing lens 246 may be opposite to the second opening 242c, the excitation light source 245 may be opposite to the third opening 242d, the spectroscope 243 and the lens 241 may be disposed in the inner cavity 242a, the lens 241 may include an opposite light inlet end 241a and an opposite light outlet end 241b, the light inlet end 241a may be opposite to the light outlet end 243 of the excitation light source 245, and the spectroscope 243 may be disposed between the image receiver 244 and the self-focusing lens 246.
In the use process of the shooting module, the excitation light source 245 emits the laser beam to the light inlet end 241a of the lens 241, the laser beam is emitted from the light outlet surface of the light outlet end 241b, and as mentioned above, the light outlet surface of the light outlet end 241b forms a convex surface formed by curved rotation of a plurality of light outlet points on a cartesian coordinate system, so that a plurality of light emitted by the plurality of light outlet points can be uniformly and diffusely emitted to the beam splitter 243, the beam splitter 243 reflects the stimulus light to the self-focusing lens 246, the stimulus light passes through the self-focusing lens 246 to the living brain, and the GCaMP virus of the living brain emits green fluorescence after being stimulated by the stimulus light and sequentially passes through the self-focusing lens 246 and the beam splitter 243 to the image receiver 244, thereby generating a brain fluorescent image.
In the above-described configuration, since the lens 241 expands the irradiation area of the stimulation light to the living brain, the size of the brain fluorescent image generated on the image receiver 244 is enlarged by enlarging the light beam of the green fluorescence reflected by the living brain, and at the same time, the stimulation light beam is uniformly and widely irradiated to the living brain, that is, the GCaMP virus of the living brain is irradiated with the light beam of the stimulation light beam of the uniform irradiation intensity, and the green fluorescence generated by the stimulation of the GCaMP virus of the living brain is also uniform, thereby improving the clarity of the brain fluorescent image.
In this embodiment of the present application, the photographing module may further include a fluorescence filter, specifically, the fluorescence filter may be disposed in the inner cavity 242a, and the fluorescence filter may be located between the beam splitter 243 and the image receiver 244, where the fluorescence filter is used for only green fluorescence to pass through, so as to avoid interference caused by transmission of light rays of other colors to the image receiver 244, which leads to generation of brain fluorescence images.
In this embodiment, the shooting module may further include an achromatic lens 247, specifically, the achromatic lens 247 may be disposed in the inner cavity 242a, and the achromatic lens 247 may be located between the beam splitter 243 and the self-focusing lens 246, where the achromatic lens 247 is used to eliminate chromatic aberration and spherical aberration of green fluorescence or thorn laser, where chromatic aberration is generated during transmission of the stimulus light and green fluorescence, where chromatic aberration is caused by chromatic dispersion and refractive index differences of light with different wavelengths in glass, so that light with different wavelengths has different focuses; the spherical aberration is a light beam emitted by an on-axis object point, and after passing through the optical system, the light beams with different angles clamped with the optical axis intersect the optical axis at different positions, so that a circular diffuse spot is formed on the image plane. The above structure can improve the resolution of the image receiver 244.
In this embodiment, the shooting module may further include a focusing lens 248, specifically, the focusing lens 248 may be disposed in the inner cavity 242a, and the focusing lens 248 may be located between the beam splitter 243 and the self-focusing lens 246, where the focusing lens 248 can adjust a small deviation such as decentration of the green fluorescence or the laser light, so that most of the light of the green fluorescence or the laser light can pass through the focusing lens 248 to reach a designated position.
In this embodiment of the present application, the shooting module may further include a liquid lens 249 and a controller, specifically, the liquid lens 249 may be disposed in the inner cavity 242a, the controller may be disposed on the housing 242, the liquid lens 249 may be located between the beam splitter 243 and the self-focusing lens 246, the controller may be electrically connected with the liquid lens 249, and the controller may control the liquid lens 249 to deform when the liquid lens 249 is in an energized state, wherein the liquid lens 249 uses the liquid as the lens 241 to change the focal length by changing the curvature of the liquid, so as to achieve automatic focusing and zooming of the portable single photon calcium imaging device, thereby improving the image effect shot by the portable single photon calcium imaging device.
In this embodiment of the present application, the shooting module may further include a battery, specifically, the battery may be disposed on the housing 242, and the image receiver 244 and the liquid lens 249 may be electrically connected with the battery, so that the portable single photon calcium imaging camera device may be disconnected from the power source connected through the electrical connector, so that the portable single photon calcium imaging camera device may be convenient for living body to move freely.
In this embodiment of the present application, the light inlet surface of the light inlet end 241a may be adhered with a blue light filter layer 241c, and since the blue light band in the thorn laser can generate green fluorescence for exciting the GCaMP virus, the blue light filter layer 241c can only allow the blue light in the thorn laser to pass through, thereby avoiding the adverse effect caused by the light of other color bands irradiating to the living brain.
In this embodiment of the present application, the lens 241 may establish a cartesian coordinate system with the position of the light source as the origin, the fitted curve fitted by the plurality of lens surface points may be rotated to form a closed three-dimensional model, the closed three-dimensional model is the lens 241, and the coordinate positions of the plurality of lens surface points in the cartesian coordinate system may be obtained by the following formula:
wherein X is the X-axis coordinate value of any one of the plurality of lens surface points on a Cartesian coordinate system, Y is the Y-axis coordinate value of any one of the plurality of lens surface points on the Cartesian coordinate system, and N ix N is the normal component of any one of a plurality of lens profile surface points in the X-axis direction on a cartesian coordinate system iy For the normal component of any one of a plurality of lens profile surface points in the Y-axis direction on a Cartesian coordinate system, I (i+1)x A unit vector of an X-axis direction of incident light on a Cartesian coordinate system for matching any one of a plurality of lens profile surface points, I (i+1)y A unit vector, θ, in the Y-axis direction on a Cartesian coordinate system for incident light to which any one of a plurality of lens profile surface points is matched j For any one of a plurality of lens profile surface points, P ix X-axis coordinate value, P, on Cartesian coordinate system for an incident point of incident light to which any one of a plurality of lens profile surface points is matched iy The Y-axis coordinate values on a cartesian coordinate system for an incident point of incident light to which any one of a plurality of lens profile surface points is matched.
The radiance distribution of a light source is known, that is, the energy distribution of the light emitted by the light source is known, the light source is regarded as a point, the energy distribution of the light source can be basically approximated to an axisymmetric distribution, as shown in fig. 4 and 5, Ω is regarded as the energy distribution of the light source, S is regarded as the expected energy distribution of the light emitted by the light source, Ω and S are respectively classified into energy grids, and for the classification of Ω, since the energy distribution is known, it is ensured that the energy in each grid is equal at the time of the classification; for the division of S, the equality of the area within each grid is also ensured at the time of division.
The grid division calculates the profile of the lens 241, so that the light energy distribution effect achieved by the lens 241 is the energy distribution on S, and the boundaries between Ω and S are determined by the energy division, and therefore, the incident light vector I i Can be determined one by one, and the normal vector N corresponding to the point can be calculated through Snell's law i Normal vector N i The point can refract incident light rays to a curved surface normal vector at a designated position, and a plurality of lens surface points are calculated one by one through the formula by utilizing normal vector iteration.
In the use process of the lens 241, the irradiation area of the living brain needs to be irradiated by the stimulation light source, and a plurality of light rays emitted by the stimulation light source are refracted by the lens 241.
The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the utility model, which are all within the scope of the utility model. Accordingly, the scope of protection of the present utility model is to be determined by the appended claims.
Claims (4)
1. The utility model provides a living body calcium formation of image and behavior monitoring integrated system, its characterized in that, including action camera module, brain fluorescence shooting module, first judgement module and receiving module, brain fluorescence shooting module sets up in the brain of living body, brain fluorescence shooting module is used for taking the brain fluorescence image of living body, action camera module is used for taking the action image of living body, action camera module with brain fluorescence shooting module all with first judgement module communication connection, first judgement module is used for judging whether action image with brain fluorescence image is synchronous, first judgement module with receiving module communication connection the action image with under the synchronous condition of brain fluorescence image, receiving module stores in the preset time period action image with brain fluorescence image.
2. The integrated system of living calcium imaging and behavior monitoring according to claim 1, further comprising a calibration module, wherein the behavior imaging module and the brain fluorescence imaging module are both communicatively connected to the calibration module, and wherein the calibration module calibrates the acquisition times of the behavior image and the brain fluorescence image in the event that the behavior image is not synchronized with the brain fluorescence image.
3. The integrated system for calcium imaging and behavior monitoring of a living body according to claim 1, wherein the brain fluorescence shooting module comprises a first acquisition module, a second judgment module, a first control module and a shooting module, the first acquisition module is in communication connection with the second judgment module, the second judgment module is in communication connection with the first control module, the shooting module is used for shooting the brain fluorescence image, the shooting module is in communication connection with the first control module, the first acquisition module is used for acquiring the actual intensity of the stimulation light of the living body, the second judgment module is used for judging whether the actual intensity is the same as the preset intensity, and the first control module controls the actual intensity to be the same as the preset intensity under the condition that the actual intensity is different from the preset intensity.
4. The integrated system for calcium imaging and behavior monitoring of a living body according to claim 1, wherein the brain fluorescence shooting module comprises a second acquisition module, a third judgment module, a second control module and a shooting module, the second acquisition module is in communication connection with the third judgment module, the third judgment module is in communication connection with the second control module, the shooting module is used for shooting the brain fluorescence image, the shooting module is in communication connection with the second control module, the second acquisition module is used for acquiring the actual diopter shot by the living body, the third judgment module is used for judging whether the actual diopter is the same as the preset diopter, and the second control module controls the actual diopter to be the same as the preset diopter under the condition that the actual diopter is different from the preset diopter.
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