CN219039479U - Slide carrier for microscope coordinate conversion - Google Patents
Slide carrier for microscope coordinate conversion Download PDFInfo
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- CN219039479U CN219039479U CN202223085160.XU CN202223085160U CN219039479U CN 219039479 U CN219039479 U CN 219039479U CN 202223085160 U CN202223085160 U CN 202223085160U CN 219039479 U CN219039479 U CN 219039479U
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Abstract
The present utility model relates to slide carriers for microscope coordinate conversion. Comprising the following steps: the bracket body is a hollow rectangular body surrounded by two opposite long sides and two opposite short sides; a projection protruding from the inner sides of the two long sides of the bracket body toward the center of the bracket body for carrying a slide glass; a notch recessed from the inner sides of the two long sides of the bracket body in a direction away from the center of the bracket body; and the elastic poking piece is arranged at the junction of the long side and the short side of the bracket body and is used for applying pushing force to the glass slide borne on the convex block so as to fix the glass slide in the bracket body. Calibration points of the microscope coordinate system are set on the bracket for correcting the deviation of different microscope coordinate systems.
Description
Technical Field
The utility model relates to the fields of biology, medicine and materials, in particular to a bracket of a glass slide for an optical microscope.
Background
Slides are a common consumable for medical detection and biological experiments. The conventional glass slide is made of glass materials, has the thickness of about 1mm, the length and the width of about 75mm multiplied by 25mm, has good light transmittance, and is convenient to observe under a microscope when experimental materials such as microorganisms, tissue slices, animal and plant cells and the like are placed on the glass slide. The experimenter views through a microscope an image of interest of a typical cell, positive bacteria, a section of difficult tissue, etc. somewhere on the slide, which we refer to herein as the "target image". After the first observation, there may be a need to reposition the target image at that location for such operations as teaching presentation of typical cells, further study of positive bacteria, consultation of problematic tissue sections, etc. In order to mark the position of the target image, marks (such as circles, forks, points) and the like can be made on the position of the target image on the back surface of the glass slide by using a marker pen, and the method can only mark the rough position of the target image and can also be repeatedly found under the view of a microscope. The XY coordinate system of the microscope sample clamp and the object stage can be used for recording the XY coordinate of the first observation target image, and the target image can be found by adjusting the XY coordinate to the corresponding position when the target image is observed again. If the coordinate system of the microscope is the same (e.g., a review of the same microscope), the XY-axis coordinates are adjusted to the recorded XY-coordinate positions and the target image appears in the microscope field of view. However, since the microscope is used and maintained daily to cause a coordinate system deviation, or a coordinate system deviation exists between different microscopes, the XY-axis coordinates are adjusted to the recorded XY-coordinate positions, and thus the target image cannot be found easily. To locate the position of the target image on the slide, it is common practice to etch anchor points, anchor lines, anchor shapes or partition the slide with lines and shapes on the slide, etc. Although the method can help the positioning of the slide target image, special treatment is needed to be carried out on the slide, so that the cost of the slide is greatly increased, meanwhile, the etched pattern on the slide can also influence the spreading of the experimental material on the slide, and the etched pattern can also cause interference to the morphological judgment and identification of the experimental material.
Disclosure of Invention
In view of the above problems, it is an object of the present utility model to provide a slide carrier for carrying a slide for an optical microscope, and to set calibration points of a microscope coordinate system on the carrier for correcting deviations of different coordinate systems of the microscope.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
a slide carrier for microscope coordinate conversion, comprising:
the bracket body is a hollow rectangular body surrounded by two opposite long sides and two opposite short sides;
a projection protruding from the inner sides of the two long sides of the bracket body toward the center of the bracket body for carrying a slide glass;
a notch recessed from the inner sides of the two long sides of the bracket body in a direction away from the center of the bracket body; and
and the elastic poking piece is arranged at the junction of the long side and the short side of the bracket body and is used for applying thrust to the glass slide borne on the convex block so as to fix the glass slide in the bracket body.
The bracket body is made of glass, plastic or metal.
The length and width of the hollow portion of the carrier body is slightly greater than the length and width of the slide.
The distance between the top surface of the bump and the top surface of the bracket body is the same as the thickness of the glass slide.
The inner side of the long side of the bracket body is provided with a plurality of notches.
The notch is recessed in a direction at right angles to the long side of the bracket body.
The right angle point formed by the notch and the long side of the bracket body is used as a calibration point of the slide bracket.
The dimensions of the carrier body were 80mm long by 30mm wide by 2mm high, and the dimensions of the slide were 75mm long by 25mm wide by 1mm high.
The hollow portion in the bracket body has dimensions of 76mm long by 26mm wide by 2mm high.
The slide carrier carries a fully transparent slide or a slide with a labeled region.
Due to the adoption of the technical scheme, the utility model has the following advantages:
the utility model provides the calculation of coordinate conversion among coordinate systems, determines the positions of the target images in different microscope coordinate systems, can accurately trace the source to the target images through the converted coordinates, improves the efficiency of consultation, rechecking and the like, and does not influence the experimental operation of the glass slide.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the utility model. Like parts are designated with like reference numerals throughout the drawings. In the drawings:
FIG. 1 is a top view of a slide carrier;
FIG. 2 is an isometric view of a slide carrier;
FIG. 3 is a fully transparent slide carrying slide carrier;
FIG. 4 is a slide of the slide carrier carrying a labeled region;
FIG. 5 is a schematic view of an image under a microscope with calibration points located;
FIG. 6 is a schematic diagram of a microscope stage XY axis coordinate caliper;
fig. 7A and 7B are schematic diagrams of a coordinate system conversion algorithm.
The various references in the drawings are as follows:
1. a bracket body; 2. a bump; 3. a notch; 4. a right angle point; 5. an elastic poking piece; 6. and a glass slide.
Detailed Description
Exemplary embodiments of the present utility model will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present utility model are shown in the drawings, it should be understood that the present utility model may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the utility model to those skilled in the art.
According to some embodiments of the present application, a slide carrier is provided, wherein the carrier body is a hollow cuboid, and the material of the carrier body can be glass, plastic or metal, and the length and width of the hollow part are slightly larger than those of the used slide, so that the slide can be conveniently placed. The bottom of the bracket is provided with a plurality of (more than or equal to 4) lugs for bearing the glass slide, so that the glass slide is borne, the glass slide is prevented from falling off, and the distance between the top surface of the lugs and the top surface of the bracket body is the same as the thickness of the glass slide. The inner side of the long side of the bracket body is provided with a plurality of (more than or equal to 4) concave notches, the edges of the notches form right angles with the long side of the body, and the right angle point can be defined as a calibration point of the slide bracket. An elastic poking piece is arranged at one corner of the inner side of the bracket body, so that a slide placed on the bracket can be pushed to the other corner and fixed. The slide carrier carrying the slide is directly placed on the stage of the microscope for microscopic observation.
Algorithm of coordinate conversion: the XY coordinate systems of the two microscopes are a coordinate system (1) and a coordinate system (2), A, B in the coordinate system (1) is a calibration point, the D point is a target point, and the coordinates are respectively a calibration point A (X A ,Y A ) Calibration point B (X) B ,Y B ) Target point D (X D ,Y D ) The method comprises the steps of carrying out a first treatment on the surface of the The coordinates corresponding to the coordinate system (2) are the calibration points a (X) a ,Y a ) Calibration point b (X b ,Y b ) Observation point d (X d ,Y d ) The coordinates of the calibration point a and the calibration point b are recorded through observation, and the coordinates d (X) of the target point can be obtained through calculation d ,Y d ). Respectively calculating the included angle alpha between the connecting line AB, AB and the X axis of the calibration points of the two coordinate systems A And alpha a The rotation angle of the two coordinate systems is θ=α a -α A The line between the target point D and the calibration point A forms an angle beta with the coordinate system (1)X axis A Subtracting the rotation angle to obtain the connection line and coordinate of the target point D and the calibration point ASystem (2)X shaft included angle beta) a ＝β A - θ, the coordinates of the target point D in the coordinate system (2) may be found by means of a trigonometric function.
Referring to fig. 1 and 2, the bracket body 1 is a hollow cuboid with the dimensions of 80mm×30mm×2mm, is made of stainless steel, and is used for bearing standard glass slides 6 with the dimensions of 75mm×25mm×1mm. The hollow part in the bracket body 1 has the dimensions of 76mm multiplied by 26mm multiplied by 2mm, and the length and the width are larger than those of a standard glass carrier sheet by 1mm. The bottom of the bracket is provided with 8 lugs 2 for bearing glass slides, the sizes of the lugs 2 are 2mm multiplied by 1mm, the bottoms of the lugs 2 and the bottom of the bracket body 1 are on the same plane, the tops of the lugs 2 are 1mm away from the top of the bracket body 1, the glass slides 6 can be completely borne, the glass slides are prevented from falling off, and the top surface of the glass slide 6 and the top surface of the bracket body 1 are on the same plane. The inner side of the long side of the bracket body 1 is provided with 8 concave notches 3, the edges of the notches 3 form right angles with the long side of the body, and the right angle point 4 can be defined as a calibration point of the slide bracket. An elastic pulling piece 5 is arranged at one corner of the inner side of the bracket body 1, and can push and fix a glass slide 6 placed on the bracket to the other corner. Referring to fig. 3 and 4, fig. 3 is a slide carrier carrying a fully transparent slide, and fig. 4 is a slide carrier carrying a labeled region of the slide carrier. The slide holder carrying the slide 6 is directly placed on the stage of the microscope and observed under the microscope.
The stage of the microscope is adjusted with the first and third orthogonal points 4 near the slide 6 as two calibration points 4a, 4b, and the orthogonal angles of the two calibration points 4a, 4b are moved to the middle of the microscope field of view, respectively, as shown in fig. 5.
The coordinates of the microscope stage (standard configuration of microscope) were recorded as shown in fig. 6.
Two microscopes, the observer observes the abnormal chromosome karyotype of the target image at the D point position (D1-D10) of the first microscope (1)), observes and records the calibration point A, B of the bracket and the target image D point, and the coordinates are the calibration point A (120.4, 26.5), the calibration point B (172.7, 26.2) and the target point D1 (162.5, 16.8); the coordinates of A, B points in the second microscope (2)) are calibration point a (120.0, 25.4), calibration point b (174) due to the differences between the microscope coordinates.4,25.4). The data is input into an Excel applet designed according to the coordinate conversion method of the present utility model, as shown in table 1, which is an interface diagram for realizing coordinate conversion by Excel, bold fonts are observed coordinate data, and standard bold fonts are calculated results by manual input. Target point d (X) d ,Y d ) The coordinate of the target point D1 corresponding to the microscope (2) is D1 (162.15, 15.93) through calculation, the coordinate of the microscope (2) is moved to (162.2, 15.9), the abnormal nuclear type image appears in the vision, and the positioning is accurate.
TABLE 1
As shown in fig. 7A and 7B, there may be both horizontal offset and angular rotation of the two coordinate systems, calculated as follows:
angle alpha between AB and X axis A ＝arctan((Y B -Y A )/(X B -X A ))
angle alpha between ab and X axis a ＝arctan((Y b -Y a )/(X b -X a ))
Angle of rotation θ=α a -α A
Angle beta between AD and X axis A ＝arctan((Y D -Y A )/(X D -X A ))
angle beta between ad and X axis a ＝β A +θ
Distance between AD's L AD ＝((X D -X A ) 2 +(Y D -Y A ) 2 ) 1/2
Coordinate X of d point d ＝X a +L AD *cosβ a ，Y d ＝Y a +L AD *sinβ a
According to the calculation method, taking Excel software as an example (other software can be used to design coordinate conversion calculation tools), a microscope coordinate conversion calculation tool is designed as shown in table 1. The coordinates of the calibration point A, B observed by the microscope (1) were recorded in the cell (B3: C4) region, the cell (E3: F4) regionThe domain record calibration point A, B observes corresponding coordinates a, B and a G3 unit grid input formula "=ATAN ((C4-C3)/(B4-B3)) ×180/PI ()" in a microscope (2) to calculate an included angle alpha between a calibration point connecting line AB and an X axis A The G4 cell entry formula "=atan ((F4-F3)/(E4-E3)) ×180/PI ()" calculates the angle α between the calibration point line ab and the X axis a The H3 cell entry formula "=g4-G3" calculates the rotation angle θ of the two microscope coordinate systems with respect to the a calibration point. The coordinates of 10 observation points D (D1-D10) are recorded in a cell (B6: C15) area, a coordinate X value of the observation point D in the microscope (2) is calculated in a D6 cell recording formula "=ATAN ((C6-C3)/(B6-B3)) × 180/PI ()", an angle between the observation point D and a calibration point A on the line DA and the X axis is calculated in an E6 cell recording formula "= ((B6-B3)/(2+ (C6-C3) & 2) & lt 0.5)", a distance between the observation point D and the calibration point A is calculated in a G6 cell recording formula "=E 3+E6×COS (PI () (D6+H 3)/180)", a coordinate X value of the observation point D in the microscope (2) is calculated in a H6 cell recording formula "=F 3+E6×6×6×4)", and a coordinate value of the observation point D in the microscope (2) is calculated in a G6 cell recording formula "=E 7+H 7×15, E7-D15, and H15-H15, and a filling formula in the G6 cell.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present utility model, and are not limiting; although the utility model has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present utility model.
Claims (10)
1. A slide carrier for microscope coordinate conversion comprising:
the bracket body is a hollow rectangular body surrounded by two opposite long sides and two opposite short sides;
a projection protruding from the inner sides of both long sides of the bracket body toward the center of the bracket body for carrying a slide glass;
a notch recessed from the inner sides of both long sides of the bracket body in a direction away from the center of the bracket body; and
the elastic poking piece is arranged at the junction of the long side and the short side of the bracket body and is used for applying thrust to the glass slide borne on the convex block so as to fix the glass slide in the bracket body.
2. The slide carrier for microscope coordinate conversion according to claim 1, wherein the material of the carrier body is glass, plastic or metal.
3. The slide carrier for microscope coordinate conversion according to claim 1, wherein the length and width of the hollow portion of the carrier body is slightly larger than the length and width of the slide.
4. The slide carrier for microscope coordinate conversion according to claim 1, wherein a distance between a top surface of the bump and a top surface of the carrier body is the same as a thickness of the slide.
5. A slide carrier for microscope coordinate conversion according to claim 1, wherein a plurality of the notches are provided on the inner side of the long side of the carrier body.
6. The slide carrier for microscope coordinate conversion according to claim 1, wherein the notch is recessed in a direction orthogonal to a long side of the carrier body.
7. A slide carrier for microscope coordinate conversion according to claim 1, wherein a right angle point formed by the notch and the long side of the carrier body is used as a calibration point of the slide carrier.
8. The slide carrier for microscope coordinate conversion according to claim 1, wherein the carrier body has dimensions of 80mm long by 30mm wide by 2mm high, and the slide has dimensions of 75mm long by 25mm wide by 1mm high.
9. The slide carrier for microscope coordinate conversion according to claim 8, wherein the hollow portion in the carrier body has dimensions of 76mm long by 26mm wide by 2mm high.
10. The slide carrier for microscope coordinate conversion according to claim 1, wherein the slide carrier carries a fully transparent slide or a slide with a labeled zone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223085160.XU CN219039479U (en) | 2022-11-17 | 2022-11-17 | Slide carrier for microscope coordinate conversion |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223085160.XU CN219039479U (en) | 2022-11-17 | 2022-11-17 | Slide carrier for microscope coordinate conversion |
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| Publication Number | Publication Date |
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| CN219039479U true CN219039479U (en) | 2023-05-16 |
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| CN202223085160.XU Active CN219039479U (en) | 2022-11-17 | 2022-11-17 | Slide carrier for microscope coordinate conversion |
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