CN212669649U - Cell climbing sheet - Google Patents

Abstract

The utility model relates to a cell climbing sheet belongs to biotechnology experimental apparatus technical field. The cell climbing sheet is a flat transparent glass sheet and is provided with two transparent parallel surfaces, scale marks are arranged on the peripheral boundary line of one of the two transparent parallel surfaces, positioning numbers are arranged on the scale marks, and the scale marks and the numbers protrude out of the transparent plane. The utility model provides a defect problem in the design of present cell slide, provide a cell slide that can distinguish the cell slide positive and negative fast and roughly fix a position. By using the cell slide, a researcher can easily distinguish the front side and the back side to confirm the side where the cell is located, and can roughly position the interested visual field, so that the target area can be quickly locked when the researcher observes the cell next time.

Description

Cell climbing sheet

Technical Field

The utility model relates to a cell climbing sheet belongs to biotechnology experimental apparatus technical field. In the present application, one side on which the cells grow is defined as the back side of the cell slide, and the corresponding other side is defined as the front side of the cell slide.

Background

Cell slide or cover glass is one of the consumables commonly used in biological experiments, and is widely applied to experiments such as histology, immunofluorescence staining, immunohistochemical staining, frozen sections, paraffin sections, cell smears, in situ hybridization experiments and the like. The cell slide and the cover glass belong to the same consumable material, and are called different names according to different experimental conditions. The in vitro cultured cells can grow to the surface of the cell slide and then are used for immunofluorescence staining experimental observation; tissue sections or cellular fluids, after being placed on the slide, need to be coverslipped for viewing. The cell slide sheet sold in the current market is generally a round or square glass sheet, the two surfaces of which are smooth and have no any mark for positioning. Since cells are generally grown on only one side of the cell slide, all experimental manipulations are performed on the side containing the cells. During the immunofluorescence staining operation, researchers are easy to confuse the cell-containing side and the cell-free side of the glass sheet due to operation negligence, and the recognition error can lead to wrong operation, so that the whole experiment directly fails. In addition, the cell slide generally does not have any structure for positioning, and if people use a pen to mark on the cell slide, the specimen is easily damaged and the observation under the microscope is affected, so researchers cannot mark or position the interesting and wanted visual field, and need to search under the microscope again when observing next time. These defects greatly increase the experimental workload and bring much trouble to researchers.

Disclosure of Invention

The utility model aims at solving the technical problems of how to conveniently distinguish the front and the back of a cell slide and how to position the cell slide.

In order to solve the above problems, the utility model adopts the technical scheme that a cell climbing sheet is provided, which is a flat transparent glass sheet and is provided with two transparent parallel surfaces, and is characterized in that: and scale marks are arranged on the peripheral boundary line of one of the two transparent parallel surfaces and protrude out of the transparent plane.

Preferably, the graduation mark is provided with positioning numbers.

Preferably, the cell slide is provided in a circular or square shape.

Preferably, the scale mark is set to be long or short; the outermost edge boundary line of the cell climbing sheet is uniformly provided with 12 long scale marks; short scale marks which are uniformly distributed are arranged between the long scale marks.

Preferably, the circular cell slide is provided with 48 short scale marks, and 4 short scale marks are uniformly distributed between every two adjacent long scale marks; the square cell slide is provided with 16 short scale marks, and two sides of each long scale mark are respectively provided with 1 short scale mark.

Preferably, the cell slide long scale lines are sequentially marked with positioning numbers in a clockwise direction, and the positioning numbers are only arranged at four long scale lines right above, right below, right left and right of the center point of the cell slide.

Preferably, the cell climbing sheet is made of high borosilicate glass, and the thickness of the cell climbing sheet is set to be 0.19-0.22 mm; the diameter specifications of the circular cell climbing sheet are four types, namely 24mm, 20mm, 14mm and 8 mm; the square cell slide is provided with three specifications of 24mm by 24mm, 15mm by 15mm and 10mm by 10 mm.

Preferably, the surface of the cell slide is treated by super-strong adsorption technology.

Preferably, the scale and the number are both printed by black glass ink relief printing.

The utility model aims at providing a cell slide which can quickly distinguish the front and the back and roughly position the cell slide for solving the defect problem in the design of the current cell slide. By using the cell slide, a researcher can easily distinguish the front side and the back side to confirm the side where the cell is located, and can roughly position the interested visual field, so that the target area can be quickly locked when the researcher observes the cell next time.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. the cell slide of the utility model can be used to quickly and simply distinguish the front and the back of the cell slide. Researchers were advised to use the non-graduated side for cell growth to ensure that the effect of the design of the slide itself on cell growth was minimized. Of course, the graduated side can be used for cell growth as well.

2. Use the utility model discloses a cell slide can effectively carry out comparatively accurate location to the target area, and the abscissa and ordinate digit through the record correspondence department can be fixed a position when observing next time and look for fast.

3. Because scale and digit adopt relief printing, if the user, the researcher uses the one side of no scale to be used for cell growth, then the cell climbing piece has can remain certain space and not laminate completely between the one side of scale and the culture plate board bottom, and this will be favorable to the researcher to fish out the cell climbing piece from the cell culture plate, and can not take place the cell climbing piece because hydrone tension is adsorbed at the bottom of the board, the condition that difficult tweezers were got takes place.

Drawings

FIG. 1 is a front view of a circular cell slide according to an embodiment of the present invention;

FIG. 2 is a front view of a square cell slide according to an embodiment of the present invention;

reference numerals: 1. region of interest

Detailed Description

In order to make the present invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings:

as shown in fig. 1-2, the utility model provides a cell climbing sheet, which is a flat transparent glass sheet and is provided with two transparent parallel surfaces, wherein the peripheral boundary line of one of the two transparent parallel surfaces is provided with scale marks which protrude out of the transparent plane; the scale marks are provided with positioning numbers. The cell climbing sheet is round or square; the scale marks are set to be long and short; the outermost edge boundary line of the cell climbing sheet is uniformly provided with 12 long scale marks; short scale marks which are uniformly distributed are arranged between the long scale marks. The circular cell climbing sheet is provided with 48 short scale marks, and 4 short scale marks are uniformly distributed between every two adjacent long scale marks; the square cell slide is provided with 16 short scale marks, and two sides of each long scale mark are respectively provided with 1 short scale mark. The cell climbing piece long scale lines are sequentially marked with positioning numbers in the clockwise direction, and the positioning numbers are only arranged at four long scale lines right above, right below, right left and right of the center point of the cell climbing piece. The cell climbing sheet is made of high borosilicate glass, and the thickness of the cell climbing sheet is set to be 0.19-0.22 mm; the diameter specifications of the circular cell climbing sheet are 24mm, 20mm, 14mm and 8 mm; the specification of the square cell slide is provided with three types of 24mm × 24mm, 15mm × 15mm and 10mm × 10 mm. The surface of the cell slide is treated by adopting super-strong adsorption technology. The scales and numbers are printed by black glass ink relief printing.

In more detail, scales and positioning numbers are added on one surface of the cell slide, the scales are divided into two stages of long and short for region division, and the numbers are convenient for researchers to record the approximate positioning of interested visual fields. The number of the long scales is 12, the length of the long scale is 1/7 corresponding to the radius of the cell slide, the width of the long scale is 1/5 corresponding to the length of the cell slide, and the long scales are uniformly distributed on the outermost edge of the cell slide. For a circular cell slide, the included angle between the centers of two adjacent long scales is 30 degrees. For a square cell slide, the long scale is located at the quartering points on each side. The short scales are 48 (round cell slide) or 16 (square cell slide), the length of the short scale is 1/10 corresponding to the radius of the cell slide, and the width is 1/5 corresponding to the length. For the circular cell slide, 4 short scales are uniformly distributed in every two adjacent long scales, and the included angle of the circle center between every two adjacent short scales or the adjacent short scales and the long scales is 6 degrees. For a square cell slide, the short scale is located at the eight equal division point on each side, respectively. The positioning numbers are sequentially marked in the clockwise direction, and only four points (0 o' clock is used as the 1 st long scale and sequenced clockwise) right above, right below, left and right of the central point are marked.

As shown in fig. 1 and fig. 2: a cell climbing sheet is a round or square glass sheet and consists of a glass sheet body and a scale positioning system.

The utility model discloses a use as follows: for immunofluorescent staining, for example, the cell slide is placed into a culture plate with the side with the scale positioned facing down, so that cells grow mainly on a smooth side without scale. Then referring to the operation of the immunofluorescence experiment step, finally sealing the slide with the scale-positioned face up on a glass slide, and observing under a microscope. During the observation, if the desired field of view or the cell of interest is found under the mirror, the approximate orientation is recorded from the positioning numbers of the fluorescent beam hitting the vicinity of the position on the slide. For example, as shown in fig. 1, according to the position of the fluorescent beam, the approximate position of the target area 1 can be recorded as the position of the midpoint between the right upper quadrant scale 2 and the central point, or as the sector area from the right upper quadrant scale 2 to the scale 3. For a square cell slide, such as that of FIG. 2, the target area may be recorded as the upper right quadrant (1.5-2 abscissa, 3.5-4 ordinate) area. The positioning information of the target area can be recorded, so that the target area can be quickly found out at any time in the next experiment.

It will be understood that, although the terms "left", "right", etc. may be used herein to describe various elements, these elements should not be limited by these terms. These terms are only used to distinguish one element from another. For example, a left cell may be referred to as a right cell, and similarly a right cell may be referred to as a left cell, without departing from the scope of the exemplary embodiments.

The foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the present invention in any way and in any way, and it should be understood that modifications and additions may be made by those skilled in the art without departing from the scope of the present invention. Those skilled in the art can make various changes, modifications and evolutions equivalent to those made by the above-disclosed technical content without departing from the spirit and scope of the present invention, and all such changes, modifications and evolutions are equivalent embodiments of the present invention; meanwhile, any changes, modifications and evolutions of equivalent changes to the above embodiments according to the actual technology of the present invention are also within the scope of the technical solution of the present invention.

Claims (8)

1. The utility model provides a cell climbing piece, is the glass piece of flat transparent form, is equipped with transparent parallel two sides, its characterized in that: and scale marks are arranged on the peripheral boundary line of one of the two transparent parallel surfaces and protrude out of the transparent plane.

2. A cell slide according to claim 1, wherein: and positioning numbers are arranged on the scale marks.

3. A cell slide according to claim 2, wherein: the cell climbing sheet is round or square.

4. A cell slide according to claim 3, wherein: the scale marks are set to be long and short; the outermost edge boundary line of the cell climbing sheet is uniformly provided with 12 long scale marks; short scale marks which are uniformly distributed are arranged between the long scale marks.

5. A cell slide according to claim 4, wherein: the circular cell climbing sheet is provided with 48 short scale marks, and 4 short scale marks are uniformly distributed between every two adjacent long scale marks; the square cell slide is provided with 16 short scale marks, and two sides of each long scale mark are respectively provided with 1 short scale mark.

6. A cell slide according to claim 5, wherein: the cell slide is equipped with the location digit with clockwise on the long scale mark of cell slide in proper order, and the location digit is only located four long scale mark departments directly over the cell slide central point, directly under, directly left and directly right-hand.

7. A cell slide according to claim 6, wherein: the cell climbing sheet is made of high borosilicate glass, and the thickness of the cell climbing sheet is set to be 0.19-0.22 mm; the diameter specification of the circular cell climbing sheet is provided with four types of 24mm, 20mm, 14mm and 8 mm; the square cell slide is provided with three specifications of 24mm by 24mm, 15mm by 15mm and 10mm by 10 mm.

8. A cell slide according to claim 2, wherein: the scales and the numbers are printed by adopting black glass ink relief printing.

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