CN219032203U - Immune cell culture amplification device based on leukocyte filter - Google Patents
Immune cell culture amplification device based on leukocyte filter Download PDFInfo
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- CN219032203U CN219032203U CN202222997136.7U CN202222997136U CN219032203U CN 219032203 U CN219032203 U CN 219032203U CN 202222997136 U CN202222997136 U CN 202222997136U CN 219032203 U CN219032203 U CN 219032203U
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Abstract
The utility model relates to the technical field of immune cell culture, in particular to an immune cell culture amplification device based on a leukocyte filter, which comprises a blood source, the leukocyte filter, a plasma collecting bag, a culture amplification bag and a culture medium component, wherein the leukocyte filter is respectively connected with the culture amplification bag and the blood source through a first three-way valve, and the leukocyte filter is also respectively connected with the culture medium component and the plasma collecting bag through a second three-way valve. By adjusting the three-way valves, the white blood cells are separated by the white blood cell filter to obtain blood plasma, and the white blood cells attached to the white blood cell filter are eluted to the culture expansion bag by the culture medium component, so that the white blood cells can be cultured and expanded to obtain corresponding immune cells. Only one set of device is needed, and the collection of blood transfusion plasma and the culture expansion of immune cells can be realized by using the adjustment of the flow direction of a three-way valve by a user, so that the operation burden of the user is reduced.
Description
Technical Field
The utility model relates to the technical field of immune cell culture, in particular to an immune cell culture amplification device based on a leukocyte filter.
Background
White blood cells are also called immune cells, play a role in immune monitoring, immune defense and immune self-stabilization in vivo, are mainly present in blood, play roles in resisting invasion of bacteria, viruses and the like, and can timely remove pathological cells, aging cells and even cancerous cells, kill tumor cells and the like. White blood cells in blood can be subjected to in vitro culture expansion and induced differentiation under proper conditions to obtain immune cells with different functions such as CIK, NK, CTL, treg, car-T and the like required by treating diseases, and the immune cells can be used for immunotherapy.
The leukocyte filter is a device commonly used for filtering leukocytes in blood, and usually polyester fiber is used as a high-efficiency filter element material, so that the leukocytes in the blood can be separated and obtained, and the leukocyte separation rate can reach more than 99%, but in the existing device, the leukocytes in the blood are filtered by using the leukocyte filter to obtain blood plasma for transfusion, for example, a blood plasma filtering and collecting device for mechanical clinical infusion is disclosed in China patent document CN 203252973U (application number 201320238229. X), and the leukocytes in the blood are filtered by using the leukocyte filter to obtain the blood plasma for transfusion. No complete equipment is available for directly amplifying and culturing immune cells after collecting leukocytes in blood by using a leukocyte filter.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art, and provides an immune cell culture and amplification device based on a leukocyte filter, which utilizes the leukocyte filter to collect leukocytes in blood, then utilizes a flushing device to flush the leukocytes into a culture and amplification bag, and provides a culture medium component, so that a culture medium required for culturing the immune cells is conveniently injected into the amplification bag, and the purpose of rapidly preparing immune cells while obtaining blood transfusion plasma by separating the leukocytes from the blood is realized.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
the utility model provides an immune cell culture expansion device based on leukocyte filter, includes blood source, leukocyte filter, plasma collection bag, cultivates the expansion bag, and culture medium subassembly, the leukocyte filter passes through first three-way valve and connects respectively cultivate the expansion bag with the blood source, first three-way valve is used for making the leukocyte filter alternative with cultivate the expansion bag or the blood source intercommunication, the leukocyte filter still passes through the second three-way valve and connects respectively culture medium subassembly with the plasma collection bag, the second three-way valve is used for making the leukocyte filter alternative with culture medium subassembly or plasma collection bag intercommunication.
According to the device provided by the utility model, through adjustment of the three-way valves, when white blood cells are separated by the white blood cell filter to obtain blood transfusion plasma, the white blood cell filter is injected into the culture medium component from the direction opposite to the blood flow direction, and the white blood cells attached to the white blood cell filter are eluted to the culture expansion bag, so that the white blood cells can be cultured and expanded, and corresponding immune cells are obtained. Only one set of device is needed, and the collection of blood transfusion plasma and the culture expansion of immune cells can be realized by using the adjustment of the flow direction of a three-way valve by a user, so that the operation burden of the user is reduced.
Preferably, the device further comprises a wash fluid bag, a wash fluid collection bag, the wash fluid bag and the blood source being connected to the first three-way valve by a third three-way valve, the plasma collection bag and the wash fluid collection bag being connected to the second three-way valve by a fourth three-way valve.
And flushing the residual red blood cells in the leukocyte filter to a flushing fluid collecting bag through a flushing fluid bag so as to reduce the residual red blood cells in the leukocyte filter as much as possible, thereby improving the purity of the leukocytes.
Preferably, the media assembly comprises media bags containing different media. In the process of immune cell culture, different culture medium components and cytokines are generally required to be added in the culture process, and immune cells are specifically induced and amplified, so that the culture medium component is provided with culture medium bags correspondingly containing the different culture medium components and cytokines, and the culture medium bags are replaced by the corresponding culture medium bags when the culture is carried out for different time periods, so that the injection of the culture medium is carried out.
Preferably, a peristaltic pump is further arranged between the culture medium component and the second three-way valve, and the flow rate of the culture medium in the culture medium component is controlled through the peristaltic pump, so that perfusion culture is realized.
The beneficial effects of the utility model are as follows:
1. according to the device provided by the utility model, through adjustment of the three-way valves, when white blood cells are separated by the white blood cell filter to obtain blood transfusion plasma, the white blood cell filter is injected into the culture medium component from the direction opposite to the blood flow direction, and the white blood cells attached to the white blood cell filter are eluted to the culture expansion bag, so that the white blood cells can be cultured and expanded, and corresponding immune cells are obtained. Only one set of device is needed, and the collection of blood transfusion plasma and the culture expansion of immune cells can be realized by using the adjustment of the flow direction of a three-way valve by a user, so that the operation burden of the user is reduced.
2. According to the utility model, through arranging the flushing fluid collecting bag, residual red blood cells in the leukocyte filter are flushed to the flushing fluid collecting bag through the flushing fluid bag, so that the residual red blood cells in the leukocyte filter are reduced as much as possible, and the purity of the leukocytes is improved.
3. According to the utility model, the culture medium component is provided with the culture medium bags correspondingly containing different culture medium components and cytokines, and the culture medium bags are replaced with the corresponding culture medium bags when the culture medium bags are used for injecting the culture medium in different time periods, so that the immune cells can be subjected to induction expansion in a targeted manner by adding the different culture medium components and the different cytokines in the culture process.
4. According to the perfusion culture device, the peristaltic pump is arranged between the culture medium component and the second three-way valve, so that the flow speed of the culture medium in the culture medium component is effectively controlled, and perfusion culture is realized.
Drawings
FIG. 1 is a schematic view of the structure of the device in example 1 provided by the present utility model;
FIG. 2 is a schematic illustration of the medium assembly of example 1 provided by the present utility model;
FIG. 3 is a schematic view of the structure of the device in embodiment 2 provided by the present utility model;
FIG. 4 is a schematic view of the structure of the device in embodiment 2 provided by the present utility model;
wherein, 11. Blood source; 12. culturing an amplification bag; 13. a leukocyte filter; 14. 141 a first media pack; 142. a second medium bag; 143. a third medium bag; 15. a plasma collection bag; 16. a rinse solution bag; 17. a rinse liquid collection bag; 21. a first three-way valve; 22. a second three-way valve; 23. a third three-way valve; 24. a fourth three-way valve; 31. peristaltic pump.
Detailed Description
The utility model will be further described with reference to the accompanying drawings and examples.
Example 1:
as shown in fig. 1, an immune cell culture expansion device based on a leukocyte filter comprises a blood source 11, a leukocyte filter 13, a plasma collection bag 15, a culture expansion bag 12 and a culture medium assembly 14, in this embodiment, the blood source 11 is a blood collection bag, other blood supply devices can be used for the blood source 11, one end of the culture expansion bag 12 and one end of the leukocyte filter 13 are connected to a first three-way valve 21, and the other end of the leukocyte filter 13, the plasma collection bag 15 and the culture medium assembly 14 are connected to a third three-way valve 23.
In this embodiment, the device components used are all existing device components, for example, a leukocyte filter provided in chinese patent document CN 208319618U (application No. 201720837684. X) can be used as the leukocyte filter, and other device component materials are not described herein.
In specific use, the device is connected as shown in fig. 1, the first three-way valve 21 is adjusted to a state in which the blood source 11 is communicated with the leukocyte filter 13, the second three-way valve 22 is adjusted to a state in which the leukocyte filter 13 is communicated with the plasma collecting bag 15, so that blood in the blood source 11 is filtered by the leukocyte filter 13, and the leukocytes are filtered out, and then the blood plasma collecting bag 15 is filled with blood transfusion plasma for blood transfusion. After the filtration of all the blood in the blood source 11 is completed, the first three-way valve 21 is adjusted to a state in which the culture expansion bag 12 is communicated with the leukocyte filter 13, and the second three-way valve 22 is adjusted to a state in which the leukocyte filter 13 is communicated with the culture medium assembly 14, as shown in fig. 2, in this embodiment, the culture medium assembly 14 includes a first culture medium bag 141, a second culture medium bag 142 and a third culture medium bag 143, which respectively contain the culture medium components and cytokines required for culturing and expanding the different stages, the first culture medium bag 141 is connected with the second three-way valve 22, the leukocytes in the leukocyte filter 13 are eluted by the material in the first culture medium bag 141 and collected into the culture expansion bag 12, but due to the adhesion of the leukocyte filter 13, a part of the leukocytes may not be effectively eluted, so that the leukocyte filter 13 is communicated with the culture medium 143 and the leukocytes in the two need to be put into the culture device for culturing, and the corresponding leukocyte filter 13 and the material in the second culture medium bag 142 and the third culture medium bag are injected into the culture medium bag according to the need, and the material for obtaining the corresponding leukocyte filter 13 through the immune culture medium bag 13.
It should be noted that the device provided by the utility model is a disposable device, and after the collection of the leucocytes and the culture and amplification of the immunocytes are carried out, the device is destroyed harmlessly after the immunocytes are obtained.
Example 2:
the immunocyte culturing and amplifying device based on a leukocyte filter, as shown in fig. 2, is different from embodiment 1 in that the device further comprises a rinsing liquid bag 16, a rinsing liquid collecting bag 17, the rinsing liquid bag 16 and the blood source 11 are connected to a first three-way valve 21 through a third three-way valve 23, and the plasma collecting bag 15 and the rinsing liquid collecting bag 17 are connected to a second three-way valve 22 through a fourth three-way valve 24.
Specifically, in the leukocyte filtering process, the device is connected in the state of the blood source 11-the third three-way valve 23-the first three-way valve 21-the leukocyte filter 13-the second three-way valve 22-the fourth three-way valve 24-the plasma collecting bag 15, after the completion of the blood filtration in the blood source 11, the device is connected in the state of the flushing fluid bag 16-the third three-way valve 23-the first three-way valve 21-the leukocyte filter 13-the second three-way valve 22-the fourth three-way valve 24-the flushing fluid collecting bag 17, and the flushing fluid in the flushing fluid bag 16 is used for flushing the residual erythrocytes and blood in the leukocyte filter 13 by using physiological saline and collecting the flushing fluid waste liquid with the flushing fluid collecting bag 17, and after flushing, the device is connected in the state of the medium assembly 14-the second three-way valve 22-the leukocyte filter 13-the first three-way valve 21-the culture expansion bag 12, and the subsequent culture expansion operation is the same as in example 1.
Example 3:
an immune cell culture expansion device based on a leukocyte filter is shown in fig. 3, and the difference between the immune cell culture expansion device and the embodiment 2 is that a peristaltic pump 31 is further arranged between the culture medium assembly 14 and the second three-way valve 22, and the peristaltic pump is used for controlling the flow rate of the culture medium in the culture medium assembly to realize perfusion culture.
Claims (4)
1. The utility model provides an immune cell culture expansion device based on leukocyte filter, its characterized in that includes blood source (11), leukocyte filter (13), plasma collection bag (15), cultivates expansion bag (12), and culture medium subassembly (14), leukocyte filter (13) are connected respectively through first three-way valve (21) cultivate expansion bag (12) with blood source (11), first three-way valve (21) are used for making leukocyte filter (13) alternative with cultivate expansion bag (12) or blood source (11) intercommunication, leukocyte filter (13) still are connected respectively through second three-way valve (22) culture medium subassembly (14) with plasma collection bag (15), second three-way valve (22) are used for making leukocyte filter (13) alternative with culture medium subassembly (14) or plasma collection bag (15) intercommunication.
2. The leukocyte-filter-based immune cell culture expansion device according to claim 1, further comprising a wash fluid bag (16), a wash fluid collection bag (17), said wash fluid bag (16) and said blood source (11) being connected to a first three-way valve (21) by a third three-way valve (23), said plasma collection bag (15) and said wash fluid collection bag (17) being connected to a second three-way valve (22) by a fourth three-way valve (24).
3. The leukocyte-filter-based immune cell culture expansion device of claim 1, wherein said medium assembly (14) comprises a medium bag containing different culture fluids.
4. The leukocyte-filter-based immune cell culture expansion device according to claim 1, characterized in that a peristaltic pump (31) is provided between said culture medium assembly (14) and the second three-way valve (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222997136.7U CN219032203U (en) | 2022-11-10 | 2022-11-10 | Immune cell culture amplification device based on leukocyte filter |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222997136.7U CN219032203U (en) | 2022-11-10 | 2022-11-10 | Immune cell culture amplification device based on leukocyte filter |
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| Publication Number | Publication Date |
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| CN219032203U true CN219032203U (en) | 2023-05-16 |
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| CN202222997136.7U Active CN219032203U (en) | 2022-11-10 | 2022-11-10 | Immune cell culture amplification device based on leukocyte filter |
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- 2022-11-10 CN CN202222997136.7U patent/CN219032203U/en active Active
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