CN219029844U - ELISA plate liquid injection device integrating upper plate and liquid injection - Google Patents
ELISA plate liquid injection device integrating upper plate and liquid injection Download PDFInfo
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- CN219029844U CN219029844U CN202223298786.9U CN202223298786U CN219029844U CN 219029844 U CN219029844 U CN 219029844U CN 202223298786 U CN202223298786 U CN 202223298786U CN 219029844 U CN219029844 U CN 219029844U
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- elisa plate
- microplate reader
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Abstract
The utility model discloses an ELISA plate liquid injection device integrating an upper plate and liquid injection, which relates to the technical field of injecting diagnostic reagents into an ELISA plate, wherein an upper plate mechanism and a flat belt conveying device are sequentially arranged on a table surface of a backing plate and below a beam of a portal frame, a liquid outlet system and a lifting mechanism for lifting the ELISA plate are further arranged on the bottom surface of the beam of the portal frame, the liquid outlet system and the lifting mechanism are both arranged right above a flat belt of the flat belt conveying device, and the lifting mechanism is arranged on the right side of the liquid outlet system; the plate feeding mechanism comprises a stock bin arranged below the cross beam and a platform fixedly arranged on the backing plate; the lifting mechanism comprises a lifting cylinder fixedly arranged on the bottom surface of the cross beam, a baffle is welded on the acting end of a piston rod of the lifting cylinder, and a vacuum disc is welded on the right end face of the baffle. The beneficial effects of the utility model are as follows: the ELISA plate is prevented from shaking in the liquid injection process, and labor cost is saved.
Description
Technical Field
The utility model relates to the technical field of injection of diagnostic reagents into an ELISA plate, in particular to an ELISA plate liquid injection device integrating an upper plate and liquid injection.
Background
The structure of a certain ELISA plate is shown in figures 1-2, and comprises a plate body, wherein a plurality of grooves 1 are formed in the top surface of the plate body. Before the use of the microplate, a certain amount of diagnostic reagent is injected into each well 1 of each microplate. The liquid injection device in a workshop comprises a flat belt conveying device, a liquid outlet system is arranged right above a flat belt of the flat belt conveying device, a material blocking cylinder is arranged on a rack of the flat belt conveying device, and the operation method for injecting diagnostic reagent into a groove 1 of an ELISA plate by using the liquid injection device is as follows: the worker starts the flat belt conveying device, the flat belt conveying device drives the flat belt to rotate, then the worker controls the piston rod of the material blocking cylinder to extend out, the piston rod extends to the upper side of the flat belt, then the worker places the ELISA plate on the flat belt, the flat belt moves the ELISA plate towards the piston rod direction of the material blocking cylinder, the ELISA plate is just blocked by the piston rod, the ELISA plate is just under the liquid outlet system, then the worker opens the liquid outlet system, and diagnostic reagents discharged by the liquid outlet system enter the grooves 1, so that the diagnostic reagents are injected into the grooves 1 of the ELISA plate; after the injection is finished, a worker controls a piston rod of the material blocking cylinder to retract, the ELISA plate continues to be conveyed along with the flat belt, and finally the worker takes the ELISA plate away from the flat belt; the diagnostic reagent can be continuously injected into the grooves 1 in the plurality of ELISA plates by repeating the above-described operation.
However, such a priming device, while capable of injecting diagnostic reagents into the microplate, still suffers from the following technical drawbacks in practice:
I. the ELISA plate is blocked by the piston rod of the material blocking cylinder, but the flat belt does not stop moving, so that the ELISA plate shakes, and then the diagnostic reagent injected into the groove 1 shakes, and even spills to the outside of the ELISA plate, so that the technical defect of inaccurate liquid injection amount exists.
II. The enzyme-labeled plates are required to be manually placed on the flat belt of the flat belt conveying device one by one, which clearly increases the workload of workers and further increases the labor cost. Therefore, there is a need for an enzyme-labeled plate priming device that prevents the enzyme-labeled plate from shaking during the priming process and saves labor costs.
Disclosure of Invention
The utility model aims to overcome the defects of the prior art and provide the ELISA plate liquid injection device which has the advantages of compact structure, shake prevention of the ELISA plate in the liquid injection process, labor cost saving, and simplicity in operation and integrates the upper plate and the liquid injection.
The aim of the utility model is achieved by the following technical scheme: the liquid injection device for the ELISA plate integrates an upper plate and liquid injection, and comprises a portal frame fixedly arranged on the table surface of a base plate, wherein an upper plate mechanism and a flat belt conveying device are sequentially arranged on the table surface of the base plate and below a cross beam of the portal frame, a liquid outlet system and a lifting mechanism for lifting the ELISA plate are further arranged on the bottom surface of the cross beam of the portal frame, the liquid outlet system and the lifting mechanism are both arranged right above a flat belt of the flat belt conveying device, and the lifting mechanism is arranged on the right side of the liquid outlet system;
the upper plate mechanism comprises a bin arranged below the cross beam and a platform fixedly arranged on the base plate, the bin is arranged right above the platform, openings are formed in the upper portion and the lower portion of the bin, a gap is formed between the lower opening of the bin and the table top of the platform, the height of the gap is equal to the thickness of the ELISA plate, the inner cavity of the bin is matched with the outer contour of the ELISA plate, a pushing cylinder positioned on the left side of the gap is fixedly arranged on the table top of the platform, an L plate is arranged at the acting end of a piston rod of the pushing cylinder, a vertical plate of the L plate is fixedly arranged at the acting end of the piston rod of the pushing cylinder, and a horizontal plate of the L plate is arranged right above the cylinder barrel of the pushing cylinder;
the lifting mechanism comprises a lifting cylinder fixedly arranged on the bottom surface of the cross beam, a baffle is welded on the acting end of a piston rod of the lifting cylinder, a vacuum disc is welded on the right end face of the baffle, a plurality of vacuum holes communicated with the inner cavity of the vacuum disc are formed between the vacuum disc and the baffle, and the vacuum disc is connected with a vacuum pump.
The left and right outer side walls of the storage bin are welded with connecting frames, and the upper ends of the two connecting frames are welded on the cross beam.
Guard plates are fixedly arranged on the front side and the rear side of the frame of the flat belt conveying device, and the distance between the two guard plates is equal to the longitudinal width of the ELISA plate.
The right end of the vacuum disc is provided with a connector communicated with the vacuum disc, and the other end of the connector is connected with a vacuum pump through a hose.
The liquid outlet system comprises upright posts welded on the bottom surface of the cross beam, a fixed plate is fixedly arranged at the bottom of each upright post, a plurality of liquid outlet pipes which correspond to grooves on the ELISA plate respectively are fixedly arranged on the fixed plate, and a constant delivery pump is connected to the other port of each liquid outlet pipe.
The table top of the platform is flush with the flat belt of the flat belt conveying device.
The liquid injection device also comprises a controller, wherein the controller is electrically connected with the electromagnetic valve of the lifting cylinder, the electromagnetic valve of the pushing cylinder, the vacuum pump and the quantitative pump through signal wires.
The utility model has the following advantages: the ELISA plate is prevented from shaking in the liquid injection process, and labor cost is saved.
Drawings
FIG. 1 is a schematic diagram of an ELISA plate;
FIG. 2 is a top view of FIG. 1;
FIG. 3 is a schematic diagram of the structure of the present utility model;
FIG. 4 is an enlarged schematic view of part I of FIG. 3;
FIG. 5 is a left side view of FIG. 4;
FIG. 6 is a schematic structural view of the upper plate mechanism;
FIG. 7 is a schematic diagram of a liquid outlet system;
FIG. 8 is a schematic view of the A-direction of FIG. 3;
FIG. 9 is a schematic view of the extension of the piston rods of the pushing cylinder and the lifting cylinder;
FIG. 10 is a schematic view of the bottommost ELISA plate I blocked by a baffle;
FIG. 11 is a schematic view of lifting the ELISA plate I;
in the figure, 1-groove, 2-backing plate, 3-portal frame, 4-upper plate mechanism, 5-flat belt conveying device, 6-liquid outlet system, 7-lifting mechanism, 8-stock bin, 9-platform, 10-pushing cylinder, 11-L plate, 12-lifting cylinder, 13-baffle, 14-vacuum disc, 15-vacuum hole, 16-connecting frame, 17-guard plate, 18-joint, 19-column, 20-fixed plate, 21-liquid outlet pipe, 22-flat belt, 23-ELISA plate and 24-ELISA plate I.
Detailed Description
The utility model is further described below with reference to the accompanying drawings, the scope of the utility model not being limited to the following:
as shown in fig. 2-8, the enzyme-labeled plate liquid injection device integrating an upper plate and liquid injection comprises a portal frame 3 fixedly arranged on the table surface of a base plate 2, an upper plate mechanism 4 and a flat belt conveying device 5 are sequentially arranged on the table surface of the base plate 2 and below a beam of the portal frame 3, a liquid outlet system 6 and a lifting mechanism 7 for lifting the enzyme-labeled plate are further arranged on the bottom surface of the beam of the portal frame 3, the liquid outlet system 6 and the lifting mechanism 7 are both arranged right above a flat belt 22 of the flat belt conveying device 5, and the lifting mechanism 7 is arranged on the right side of the liquid outlet system 6; the upper plate mechanism 4 comprises a bin 8 arranged below the cross beam and a platform 9 fixedly arranged on the base plate 2, the table top of the platform 9 is flush with a flat belt 22 of the flat belt conveying device 5, the bin 8 is arranged right above the platform 9, openings are formed in the upper and lower parts of the bin 8, gaps are formed in front of the lower openings of the bin 8 and the table top of the platform 9, the height of the gaps is equal to the thickness of the ELISA plate, the inner cavity of the bin 8 is matched with the outer contour of the ELISA plate, a pushing cylinder 10 positioned on the left side of the gaps is fixedly arranged on the table top of the platform 9, an L plate 11 is arranged at the acting end of a piston rod of the pushing cylinder 10, a vertical plate of the L plate 11 is fixedly arranged at the acting end of the piston rod of the pushing cylinder 10, and a horizontal plate of the L plate 11 is arranged right above a cylinder barrel of the pushing cylinder 10.
As shown in fig. 2-8, the lifting mechanism 7 comprises a lifting cylinder 12 fixedly arranged on the bottom surface of the beam, a baffle 13 is welded on the acting end of a piston rod of the lifting cylinder 12, a vacuum disc 14 is welded on the right end surface of the baffle 13, a plurality of vacuum holes 15 communicated with the inner cavity of the vacuum disc 14 are arranged between the vacuum disc 14 and the baffle 13, the vacuum disc 14 is connected with a vacuum pump, a joint 18 communicated with the vacuum disc 14 is arranged at the right end of the vacuum disc 14, and the other end of the joint 18 is connected with the vacuum pump through a hose.
The left and right outer side walls of the storage bin 8 are welded with connecting frames 16, and the upper ends of the two connecting frames 16 are welded on the cross beam. Guard plates 17 are fixedly arranged on the front side and the rear side of the frame of the flat belt conveying device 5, and the distance between the two guard plates 17 is equal to the longitudinal width of the ELISA plate. The liquid outlet system 6 comprises upright posts 19 welded on the bottom surface of the cross beam, a fixed plate 20 is fixedly arranged at the bottom of each upright post 19, a plurality of liquid outlet pipes 21 corresponding to the grooves 1 on the ELISA plate are fixedly arranged on the fixed plate 20, and the other port of each liquid outlet pipe 21 is connected with a constant delivery pump.
The priming device still includes the controller, the solenoid valve of controller and lift cylinder 12, the solenoid valve of pushing away material cylinder 10, vacuum pump and constant delivery pump are through signal line electricity connection, and workman accessible controller control lift cylinder 12 and pushing away the extension or the withdrawal of material cylinder 10 piston rod can also control the start-up or the closure of vacuum pump and constant delivery pump simultaneously to convenient workman's operation has the characteristics that degree of automation is high.
The working process of the utility model is as follows:
s1, a worker places a certain number of ELISA plates 23 into a bin 8 from an upper opening of the bin 8, and after placing, the ELISA plate I24 at the bottommost layer is just supported on the table top of the platform 9 and just positioned in the gap;
s2, a worker controls a motor of the flat belt conveying device 5 to start, the motor drives the flat belt 22 to start, and the flat belt 22 rotates clockwise;
s3, a worker controls a piston rod of the lifting cylinder 12 to extend downwards, the piston rod drives the baffle 13 and the vacuum disc 14 to move downwards synchronously, and when the piston rod of the lifting cylinder 12 extends completely, the bottom surface of the baffle 13 is just close to the top surface of the flat belt 22, as shown in FIG. 9;
s4, an upper plate of the ELISA plate I24: the worker controls the piston rod of the pushing cylinder 10 to extend rightwards, the piston rod drives the L plate 11 to move rightwards, the vertical plate of the L plate 11 pushes the ELISA plate I24 at the bottommost layer to move rightwards along the table surface of the platform 9, the horizontal plate of the L plate 11 blocks the ELISA plate 23 at the penultimate layer so as to prevent the ELISA plate 23 at the penultimate layer from falling onto the platform 9, and when the piston rod of the pushing cylinder 10 extends completely, the ELISA plate I24 is just pushed onto the flat belt 22, so that the upper plate of the ELISA plate I24 is realized as shown in fig. 9; after the plate is arranged, a worker controls the piston rod of the pushing cylinder 10 to retract, and the enzyme label plate 23 of the penultimate layer falls onto the platform 9 at the moment; the enzyme-labeled plate I24 falling onto the flat belt 22 moves rightward with the flat belt 22, the right end face of the enzyme-labeled plate I24 is blocked by the baffle 13, and the vacuum hole 15 is blocked by the right end face thereof, as shown in FIG. 10;
according to the step S4, the ELISA plates 23 in the storage bin 8 can be continuously and automatically pushed onto the flat belt 22 of the flat belt conveying device 5 through the reciprocating extension or retraction movement of the piston rod of the pushing cylinder 10, compared with a liquid injection device in a workshop, the ELISA plates are not required to be placed onto the flat belt one by one manually, so that the workload of workers is reduced, and the labor cost of enterprises is further saved.
S5, injecting liquid into the ELISA plate I24, wherein the specific operation steps are as follows:
s51, a worker controls a vacuum pump to start, the vacuum pump vacuumizes the vacuum holes 15 and the inner cavity of the vacuum disc 14, and the ELISA plate I24 is adsorbed on the baffle 13 under negative pressure;
s52, a worker controls a piston rod of the lifting cylinder 12 to extend upwards, the piston rod drives the baffle 13 to move upwards, the baffle 13 drives the absorbed ELISA plate I24 to move upwards, and after the piston rod of the lifting cylinder 12 is completely retracted, each groove 1 on the ELISA plate I24 is just under the liquid outlet pipe 21, as shown in FIG. 11;
s53, a worker controls each quantitative pump to start, the quantitative pumps pump diagnostic reagents in the diagnostic reagent storage tanks, and the pumped diagnostic reagents flow into the grooves 1 through the liquid outlet pipes 21, so that liquid injection to the ELISA plate I24 is finally realized;
s5, after the liquid injection of the ELISA plate I24 is finished, a worker controls a piston rod of the lifting cylinder 12 to extend downwards, the piston rod drives the absorbed ELISA plate I24 to move downwards, when the bottom surface of the ELISA plate I24 contacts the top surface of the flat belt 22, the worker controls a vacuum pump to be closed, then controls the piston rod of the lifting cylinder 12 to retract, the piston rod drives the baffle 13 to move upwards, and when the baffle 13 is separated from the ELISA plate I24, the ELISA plate I24 injected with the diagnostic reagent continues to be conveyed rightwards;
in step S5, the elisa plate I24 is firstly adsorbed by the vacuum plate 14, and then the elisa plate I24 is lifted by the lifting cylinder 12, so that the elisa plate I24 is far away from the moving flat belt 22, compared with a liquid injection device used in a workshop, the shake of the elisa plate I24 in the liquid injection process is effectively prevented, the shake of the diagnostic reagent injected into the groove 1 is prevented, and the drop of the diagnostic reagent outside is avoided, so that the liquid injection device has the technical characteristic of more accurate liquid injection.
S6, the worker repeats the operations of the steps S4-S5, and the ELISA plates 23 in the storage bin 8 can be continuously conveyed to the liquid injection station of the liquid outlet system 6 for liquid injection.
Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.
Claims (7)
1. The utility model provides an collect upper plate and annotate liquid in ELISA plate priming device of an organic whole, it includes portal frame (3) that set firmly on backing plate (2) mesa, its characterized in that: an upper plate mechanism (4) and a flat belt conveying device (5) are sequentially arranged on the table top of the base plate (2) and below the cross beam of the portal frame (3), a liquid outlet system (6) and a lifting mechanism (7) for lifting the ELISA plate are further arranged on the bottom surface of the cross beam of the portal frame (3), the liquid outlet system (6) and the lifting mechanism (7) are both arranged right above a flat belt (22) of the flat belt conveying device (5), and the lifting mechanism (7) is arranged on the right side of the liquid outlet system (6);
the upper plate mechanism (4) comprises a bin (8) arranged below the cross beam and a platform (9) fixedly arranged on the base plate (2), the bin (8) is arranged right above the platform (9), openings are formed in the upper portion and the lower portion of the bin (8), a gap is formed between the lower opening of the bin (8) and the front of the table top of the platform (9), the height of the gap is equal to the thickness of the ELISA plate, the inner cavity of the bin (8) is matched with the outer contour of the ELISA plate, a pushing cylinder (10) positioned on the left side of the gap is fixedly arranged on the table top of the platform (9), an L plate (11) is arranged at the acting end of a piston rod of the pushing cylinder (10), and a vertical plate of the L plate (11) is fixedly arranged at the acting end of the piston rod of the pushing cylinder (10), and a horizontal plate of the L plate (11) is arranged right above a cylinder barrel of the pushing cylinder (10);
the lifting mechanism (7) comprises a lifting cylinder (12) fixedly arranged on the bottom surface of the beam, a baffle (13) is welded on the acting end of a piston rod of the lifting cylinder (12), a vacuum disc (14) is welded on the right end face of the baffle (13), a plurality of vacuum holes (15) communicated with the inner cavity of the vacuum disc (14) are formed between the vacuum disc (14) and the baffle (13), and the vacuum disc (14) is connected with a vacuum pump.
2. The microplate reader of claim 1, wherein the microplate reader is further adapted to: connecting frames (16) are welded on the left and right outer side walls of the storage bin (8), and the upper ends of the two connecting frames (16) are welded on the cross beam.
3. The microplate reader of claim 1, wherein the microplate reader is further adapted to: guard plates (17) are fixedly arranged on the front side and the rear side of a frame of the flat belt conveying device (5), and the distance between the two guard plates (17) is equal to the longitudinal width of the ELISA plate.
4. The microplate reader of claim 1, wherein the microplate reader is further adapted to: the right end part of the vacuum disc (14) is provided with a joint (18) communicated with the vacuum disc, and the other end of the joint (18) is connected with a vacuum pump through a hose.
5. The microplate reader of claim 1, wherein the microplate reader is further adapted to: the liquid outlet system (6) comprises upright posts (19) welded on the bottom surface of the cross beam, a fixed plate (20) is fixedly arranged at the bottom of each upright post (19), a plurality of liquid outlet pipes (21) which correspond to grooves (1) on the ELISA plate respectively are fixedly arranged on the fixed plate (20), and a constant delivery pump is connected to the other port of each liquid outlet pipe (21).
6. The microplate reader of claim 1, wherein the microplate reader is further adapted to: the table top of the platform (9) is flush with the flat belt (22) of the flat belt conveying device (5).
7. The microplate reader of claim 1, wherein the microplate reader is further adapted to: the liquid injection device also comprises a controller, wherein the controller is electrically connected with the electromagnetic valve of the lifting cylinder (12), the electromagnetic valve of the pushing cylinder (10), the vacuum pump and the quantitative pump through signal wires.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223298786.9U CN219029844U (en) | 2022-12-09 | 2022-12-09 | ELISA plate liquid injection device integrating upper plate and liquid injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223298786.9U CN219029844U (en) | 2022-12-09 | 2022-12-09 | ELISA plate liquid injection device integrating upper plate and liquid injection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219029844U true CN219029844U (en) | 2023-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223298786.9U Active CN219029844U (en) | 2022-12-09 | 2022-12-09 | ELISA plate liquid injection device integrating upper plate and liquid injection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219029844U (en) |
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2022
- 2022-12-09 CN CN202223298786.9U patent/CN219029844U/en active Active
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