CN219029836U

CN219029836U - Auxiliary device for dumping solid culture medium into flat plate

Info

Publication number: CN219029836U
Application number: CN202223548284.7U
Authority: CN
Inventors: 李俊玲
Original assignee: Shandong Medical Yuan Pluripotent Stem Cell Bioengineering Co ltd
Current assignee: Shandong Medical Yuan Pluripotent Stem Cell Bioengineering Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-05-16
Anticipated expiration: 2032-12-30

Abstract

In order to solve the problems of poor uniformity, long time consumption and the like in the production of a flat culture medium, the utility model provides an auxiliary device for pouring a solid culture medium flat plate, which is characterized by comprising a liquid storage bottle, an electric sucking aid and a liquid suction pipe, wherein the electric sucking aid and the liquid suction pipe are respectively connected to the upper end and the lower end of the liquid storage bottle, scale marks are arranged on the side wall of the liquid storage bottle, a liquid outlet at the lower end of the liquid storage bottle is provided with a check plug, one side of the check plug is connected with the inner wall of the liquid storage bottle through an elastic connecting arm, in addition, a liquid outlet pipe is further arranged in the liquid storage bottle, one end of the liquid outlet pipe is positioned at the inner lower end of the liquid storage bottle, and the other end of the liquid outlet pipe is distributed from bottom to top and extends out of the liquid outlet pipe.

Description

Auxiliary device for dumping solid culture medium into flat plate

Technical Field

The utility model relates to the technical field of cell culture devices, in particular to an auxiliary device for a solid culture medium dumping plate.

Background

The plate medium is the most commonly used form of solid medium used to obtain pure culture of microorganisms, which is a solid plane of medium formed by cooling solidified solid medium in a sterile petri dish, often referred to simply as a culture plate. Also called planar medium, plate medium.

The laboratory manual process of preparing the plate medium is typically to first place the medium in an Erlenmeyer flask and then sterilize it with steam under high pressure. And cooling the culture medium to 55-60 ℃ in an ultra-clean bench, holding a conical flask and a sterile plate by one, pouring the culture medium which is not solidified into the sterile empty plate one by one, rapidly covering a plate cover, placing the plate cover on a table, lightly rotating the plate to ensure that the culture medium is uniformly distributed at the bottom of the whole plate, and condensing to obtain the plate culture medium.

In the general operation method in the prior art, the culture medium amount in each plate is visual inspection, the uniformity is poor, the time consumption is long, if the plate amount is large, the culture medium in the conical flask can be solidified in the plate pouring process, and the culture medium needs to be heated and melted and then is continued, so that the time is wasted.

Disclosure of Invention

In order to solve the problems of poor uniformity, long time consumption and the like in the production of a flat culture medium, the utility model provides an auxiliary device for dumping a flat culture medium, which adopts the following technical scheme:

a auxiliary device for solid medium emptys dull and stereotyped, its characterized in that includes stock solution bottle, electronic helping the aspirator, the pipette, electronic helping the aspirator and the pipette is connected in stock solution bottle upper end and lower extreme respectively, stock solution bottle lateral wall is provided with the scale mark, and stock solution bottle lower extreme liquid outlet department is provided with the non return stopper, non return stopper one side links to each other with the stock solution bottle inner wall through the elastic connection arm, in addition, still be provided with the drain pipe in the stock solution bottle, drain pipe one end is located the stock solution bottle lower extreme, and the other end distributes from bottom to top and stretches out to the drain pipe outside.

Preferably, the upper end of the liquid suction pipe is connected with the liquid storage bottle, the lower end of the liquid suction pipe is a liquid suction port, and a sealing plug is arranged at the outer side of the joint of the liquid suction pipe and the liquid storage bottle.

Preferably, a filter layer is arranged between the upper end of the liquid storage bottle and the electric sucking assisting device.

Preferably, one end of the liquid outlet pipe, which is positioned outside the liquid storage bottle, is connected with a rubber hose, and a switch clamp is arranged on the rubber hose.

The utility model has the beneficial effects that:

1. the device is simple and quick to operate, can be used for filling a plurality of flat plates at one time, prevents the culture medium from solidifying due to overlong operation time, and can accurately control the volume of the culture medium in each flat plate by prefabricating scale marks on the liquid storage bottle, so that the uniformity of each flat plate is ensured;

2. the liquid outlet pipe is arranged on the inner side of the pipe body, on one hand, as the liquid outlet pipe is thinner, most of the liquid outlet pipe can be positioned in the pipe body by the design, the liquid outlet pipe which extends out of the pipe body is prevented from being too long, so that a culture medium in the liquid outlet pipe is solidified to be blocked, on the other hand, the head end of the liquid outlet pipe can be positioned at the lowest point of the liquid storage bottle, and the middle section of the liquid outlet pipe is positioned above the liquid level of the pipe body, so that the culture medium in the pipe body can not naturally flow out under the condition that the tail end clip is opened, and the operation by a single person is facilitated;

3. the liquid storage bottle lower extreme opening is provided with the non return stopper, and the non return stopper passes through the elastic connection arm and links to each other with the liquid storage bottle inner wall, and the non return stopper can be opened during imbibition, and accessible elastic connection arm is with liquid storage bottle lower extreme opening shutoff during the liquid outlet, prevents that the culture medium from flowing backwards back to the erlenmeyer flask.

Drawings

FIG. 1 is a schematic view of the structure of the present utility model

FIG. 2 is a schematic diagram showing the connection structure of a liquid storage bottle, a liquid suction pipe and a liquid outlet pipe

FIG. 3 is a schematic view of the partial structure of FIG. 2

The device comprises a 1-electric sucking assisting device, a 11-electric sucking assisting device connecting pipe, a 12-filter layer, a 2-liquid storage bottle, 21-scale marks, a 22-check plug, a 23-elastic connecting arm, a 3-liquid suction pipe, a 31-liquid suction port, a 4-liquid outlet pipe, a 41-rubber hose, a 42-switch clamp and a 5-sealing plug.

Detailed Description

Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. While exemplary embodiments of the present disclosure are shown in the drawings, it should be understood that the present disclosure may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

In the description of the utility model, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the view direction or positional relationships, merely to facilitate describing the utility model, and do not indicate or imply that the devices or elements being referred to must have a particular orientation, be configured and operated in a particular orientation, and thus should not be construed as limiting the utility model.

The auxiliary device for pouring a solid culture medium into a flat plate as shown in fig. 1-3 comprises an electric sucking aid 1, a liquid storage bottle 2, a liquid suction pipe 3 and a liquid outlet pipe 4, wherein the upper end and the lower end of the liquid storage bottle 2 are respectively opened, scale marks 21 distributed along the height direction are arranged on the outer side wall of the liquid storage bottle 2, the electric sucking aid 1 is connected above the liquid storage bottle 2 through an electric sucking aid connecting pipe 11, a filter layer 12 is arranged on the electric sucking aid connecting pipe 11, sterile cotton is adopted as the filter layer 12, the lower end of the liquid suction pipe 3 is a liquid suction port, the upper end of the liquid suction pipe is connected with the opening of the lower end of the liquid storage bottle 2, a sealing plug 5 is sleeved outside the joint of the upper end of the liquid suction pipe 3 and the lower end of the liquid storage bottle 2, one end of the liquid outlet pipe 4 is positioned at the lower end inside the liquid storage bottle 2, the other end of the liquid outlet pipe 4 extends out of the liquid storage bottle 2 from bottom to top along the inside of the liquid storage bottle 2 for a certain length, a rubber hose 41 is connected to one end of the liquid outlet pipe 4 extending out of the liquid storage bottle 2, and a switch clip 42 is arranged on the rubber hose 41 for controlling on-off of the rubber hose 41.

In addition, still be provided with the non return stopper 22 in liquid storage bottle 2 lower extreme opening part, the whole and liquid storage bottle 22 lower extreme opening joint of non return stopper 22 can be used to shutoff liquid storage bottle 2 lower extreme opening, and non return stopper 22 upper end one side fixedly connected with elastic connection arm 22, elastic connection arm 22 and liquid storage bottle 2 inner wall fixed connection.

The device operates as follows:

before using, the whole device is sterilized, insert the liquid absorbing mouth 31 one end of the liquid absorbing pipe 3 in the purifying workbench and be equipped with the high temperature and high pressure sterilization of culture medium and temperature drop to the erlenmeyer flask of suitable temperature, pass through sealing plug 5 and connect this device with triangle bottleneck sealing connection, electronic help the aspirator 1 to link to each other with the upper end opening of stock solution bottle 2 through electronic help aspirator connecting pipe 11, utilize electronic help aspirator 1 to carry out the imbibition operation, at this moment, because the suction of electronic help aspirator 1, the non return plug 22 upwards moves, no longer blocks up stock solution bottle 2 lower extreme opening, the culture medium gets into stock solution bottle 2 inside, until stock solution bottle 2 inside holds the culture medium of suitable volume, at this moment electronic help aspirator 1 stops the imbibition operation, non return plug 22 is under the effect of elastic connection arm 23 to block up stock solution bottle 2 lower extreme opening again, prevent the culture medium backward flow in the stock solution bottle 2, then, take off switch clamp 42, place the culture dish that is ready below rubber hose 41, utilize electronic help aspirator 1 to carry out the operation, the culture medium in the stock solution bottle 2 flows out through stock solution pipe 4 and rubber hose 41 to the stock solution bottle 2, can continue filling to the scale mark after the culture dish 2, can continue filling to the culture medium, finish the scale filling is continued.

The foregoing is only a preferred embodiment of the utility model, it being noted that: it will be apparent to those skilled in the art that modifications may be made without departing from the principles of the utility model, and such modifications are intended to be within the scope of the utility model.

Claims

1. A auxiliary device for solid medium emptys dull and stereotyped, its characterized in that includes stock solution bottle, electronic helping the aspirator, the pipette, electronic helping the aspirator and the pipette is connected in stock solution bottle upper end and lower extreme respectively, stock solution bottle lateral wall is provided with the scale mark, and stock solution bottle lower extreme liquid outlet department is provided with the non return stopper, non return stopper one side links to each other with the stock solution bottle inner wall through the elastic connection arm, in addition, still be provided with the drain pipe in the stock solution bottle, drain pipe one end is located the stock solution bottle lower extreme, and the other end distributes from bottom to top and stretches out to the drain pipe outside.

2. The auxiliary device for the solid culture medium dumping flat plate according to claim 1, wherein the upper end of the liquid suction pipe is connected with the liquid storage bottle, the lower end of the liquid suction pipe is a liquid suction port, and a sealing plug is arranged outside the joint of the liquid suction pipe and the liquid storage bottle.

3. The auxiliary device for a solid medium pouring plate according to claim 1, wherein a filter layer is arranged between the upper end of the liquid storage bottle and the electric sucking aid.

4. The auxiliary device for a solid medium dumping plate according to claim 1, wherein one end of the liquid outlet pipe, which is positioned outside the liquid storage bottle, is connected with a rubber hose, and a switch clamp is arranged on the rubber hose.