CN218938189U - All-solid-state ion selective electrode - Google Patents
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- CN218938189U CN218938189U CN202222594723.1U CN202222594723U CN218938189U CN 218938189 U CN218938189 U CN 218938189U CN 202222594723 U CN202222594723 U CN 202222594723U CN 218938189 U CN218938189 U CN 218938189U
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Abstract
The utility model provides an all-solid-state ion selective electrode. Five detection sites are arranged on the front surface of the electrode, and comprise a reference site, an auxiliary site, two detection sites and a calibration site; the detection site is provided with a corresponding microelectrode, the microelectrode comprises a working electrode, an external reference electrode and an auxiliary electrode, and the working electrode comprises a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion selective permeable membrane (26); five electrode contact sites are arranged on the back of the electrode; the front and back surfaces of the electrode are provided with five conducting sites conducting on two sides, and the detection sites and the electrode contact sites are connected to the conducting sites through metal lines and are correspondingly connected one by one, so that the purpose of generating specific response to specific ions such as potassium ions in the biochemical electrolyte is realized.
Description
Technical Field
The utility model belongs to the field of biological sensors, and particularly relates to an all-solid-state ion selective electrode.
Background
The current most novel POCT chemical sensing instrument product can realize that a plurality of samples are detected simultaneously, so that the detection time is greatly saved, and meanwhile, the instant detection is faster. POCT devices are mostly realized by using a biosensor, and the biological enzyme molecules are immobilized on a solid phase interface of a micro-analysis device, and after the analyte is specifically identified, the detection is performed by using an electrochemical or optical method, and the reading is immediately given. Typical biosensor-based commercial POCT devices are represented by blood glucose meters, blood gas analyzers, and are mainly used for blood glucose monitoring, blood gas and electrolyte analysis.
Aiming at a biochemical detection consumable device for simultaneously detecting a plurality of material indexes at one time, related technology exists at present, and specifically, microelectrodes for detecting specific biochemical molecules are integrated on an integral detection device and are applied to multi-parameter electrochemical detection. The technology has the biggest limitation that a plurality of electrode detection elements are processed on the same device, the processing steps are complex, the difficulty is high, the cost is high, meanwhile, the detection of a plurality of indexes also causes the complexity of the internal circuit of the detection device, and meanwhile, the detection has the problem that different detection points interfere with each other, so that the sensitivity and the accuracy cannot be ensured.
Therefore, the utility model aims to provide an electrochemical detection chip which reduces the cost and processing difficulty of the chip, ensures simultaneous detection of multiple parameters and improves sensitivity and accuracy on the basis of multi-parameter electrochemical detection, and realizes simultaneous detection of multiple indexes in biochemical electrolyte.
Disclosure of Invention
The utility model aims to solve the problems of realizing the selective detection of trace potassium ions in biochemical electrolyte and improving the sensitivity and accuracy of the detection. In view of this, the present utility model provides an all-solid ion-selective electrode.
The utility model aims to provide an all-solid-state ion selective electrode, wherein the front surface of the electrode is provided with five detection sites, and the five detection sites comprise a reference site, an auxiliary site, two detection sites and a calibration site; the detection site is provided with a corresponding microelectrode, and the microelectrode comprises a working electrode, an external reference electrode and an auxiliary electrode, wherein the working electrode comprises a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion selective permeable membrane (26).
Five electrode contact sites are arranged on the back of the electrode; the front and the back of the electrode are provided with five conducting sites conducting on two sides, and the detection sites and the electrode contact sites are connected to the conducting sites through metal lines and are correspondingly connected one by one.
Further, the surface of the microelectrode is modified with a plurality of layers of ink to form an ink dam in the shape of concentric circles, the diameter of the concentric circles is 0.2-1.5 mm, and the center distance of the concentric circles is 20-150 mu m; the ink box dam is processed by PCB solder resist ink, and the thickness of the ink box dam is 20-50 mu m.
Further, the electrodes are provided with positioning holes (16), and the positioning holes (16) are positioned at the upper left corner and the upper right corner of the electrodes.
Fig. 1 is a schematic front view of an all-solid-state ion selective electrode, and fig. 3 is a schematic front view of a circuit structure of the all-solid-state ion selective electrode.
Five microelectrodes are arranged on the front surface of the electrode, and comprise a first microelectrode (1) serving as a reference electrode, a second microelectrode (2) serving as an auxiliary electrode, a third microelectrode (3) serving as a working electrode, a fourth microelectrode (4) and a fifth microelectrode (5), wherein the fifth microelectrode (5) is a calibration electrode; the first microelectrode (1) is connected to a first conduction point (6) through a metal circuit, the second microelectrode (2) is connected to a second conduction point (7) through a metal circuit, the third microelectrode (3) is connected to a third conduction point (8) through a metal circuit, the fourth microelectrode (4) is connected to a fourth conduction point (9) through a metal circuit, and the fifth microelectrode (5) is connected to a fifth conduction point (10) through a metal circuit.
Fig. 2 is a schematic diagram of the back surface of the all-solid-state ion selective electrode, and fig. 4 is a schematic diagram of the back surface of the circuit structure of the all-solid-state ion selective electrode.
Five electrode contact sites are arranged on the back of the electrode, and comprise a first electrode contact site (11), a second electrode contact site (12), a third electrode contact site (13), a fourth electrode contact site (14) and a fifth electrode contact site (15), wherein the first electrode contact site (11) is connected to a first conducting point (6) through a metal circuit, the second electrode contact site (12) is connected to a second conducting point (7) through a metal circuit, the third electrode contact site (13) is connected to a third conducting point (8) through a metal circuit, the fourth electrode contact site (14) is connected to a fourth conducting point (9) through a metal circuit, and the fifth electrode contact site (15) is connected to a fifth conducting point (10) through a metal circuit; the first microelectrode (1) is connected with the first electrode contact site (11) through a first conduction point (6), the second microelectrode (2) is connected with the second electrode contact site (12) through a second conduction point (7), the third microelectrode (3) is connected with the third electrode contact site (13) through a third conduction point (8), the fourth microelectrode (4) is connected with the fourth electrode contact site (14) through a fourth conduction point (9), and the fifth microelectrode (5) is connected with the fifth electrode contact site (15) through a fifth conduction point (10).
As shown in fig. 5, a cross-sectional view of a microelectrode in the all-solid-state ion-selective electrode, specifically, a cross-sectional view of the third microelectrode (3), is shown. As can be seen from the figure, the surface metal layer modification area of the third microelectrode (3) is circular, the surface of the third microelectrode (3) is modified with a first layer of ink (17), the area except for the detection site of the selective electrode surface and the area of the electrode edge part are mainly covered, and the electrode layer which is not covered by the ink and the ink surrounding dam form a first layer of electrolytic cell (20); the surface of the first layer of ink (17) is continuously modified with the second layer of ink (18), and a surrounding dam of the second layer of ink forms a second layer of electrolytic cell (21) on the basis of the first layer of electrolytic cell (20); and a third layer of ink (19) is modified on the surface of the second layer of ink (18), a surrounding dam of the third layer of ink (19) forms a third layer of electrolytic cell (22) on the basis of the first layer of electrolytic cell (20) and the second layer of electrolytic cell (21), and the third layer of electrolytic cell presents a concentric circular structure.
Further, the surface of the base electrode (24) of the working electrode is modified with noble metals, including gold electrodes and platinum electrodes.
Further, the internal reference electrode (23) comprises a silver chloride electrode.
Further, the ion-selective permeable membrane (26) of the working electrode is composed of an ionophore, a lipophilic macromolecule, a high molecular polymer, a plasticizer, and a solvent.
Fig. 6 is a schematic structural diagram of a working electrode in the all-solid-state ion selective electrode according to the present utility model. As can be seen from the figure, the bottom layer of the working electrode is a base electrode (24), in particular a gold electrode, with a diameter of 0.8mm, on which an internal reference electrode (23), in particular a silver chloride electrode, is covered, the diameter of the electrode being 0.6mm. And then modifying the conductive polymer gel (25), wherein the diameter of the conductive polymer gel (25) is 1.2mm, and the surface of the conductive polymer gel is covered with an ion selective transmission membrane (26), and the diameter of the ion selective transmission membrane (26) is 1.5mm.
The external reference electrode of the all-solid-state ion selective electrode comprises a substrate electrode (28), a silver chloride electrode (27) and a PVC protective film (29).
Further, the surface of the base electrode (28) of the external reference electrode is modified with noble metal, including gold electrode and platinum electrode.
Further, the PVC protection film (29) of the external reference electrode is composed of a high molecular polymer, a plasticizer and a solvent.
As shown in fig. 7, a schematic structural diagram of an external reference electrode in the all-solid-state ion selective electrode is shown. As can be seen from the figure, in the external reference electrode, the base electrode (28) is a pure gold electrode, gold is used as a basal layer, the diameter of the electrode is 0.8mm, the surface of the base electrode is covered with a silver chloride electrode (27), the diameter of the electrode is 0.6mm, and the outermost layer is modified with a PVC protection film (29), and the diameter of the PVC protection film (29) is 1.7mm.
The utility model takes gold or platinum as a substrate, combines an inner reference electrode, conductive polymer gel and an ion sensitive membrane to prepare an ion selective electrode, combines the ion selective electrode with an outer reference electrode to generate specific response to specific ions such as potassium ions in biochemical electrolyte, can realize simultaneous detection of different types of specific ions or simultaneously obtain multiple detection results of the specific ions by changing the ionophore of the ion sensitive membrane, and greatly improves the accuracy and sensitivity of the electrode.
The beneficial effects of the utility model are as follows:
(1) The utility model arranges a plurality of detection points on the electrode, modifies the microelectrode, and connects the electrode contact point through the metal circuit and the conduction point, thereby greatly saving the electrode area, realizing the purpose of microelectrode integration, simplifying the structure design of the connecting circuit of the microelectrode and the electrode contact point, and reducing the processing difficulty and cost.
(2) According to the utility model, a three-electrode system is set to be five or more microelectrode integrated, and the calibration electrodes are set in the working electrodes, so that the self-calibration function of the microelectrode integrated electrode is realized, meanwhile, the working electrode serving as the calibration electrode can be also used for independently detecting a certain target object, and the stability and the sensitivity of the electrode are greatly increased on the basis of simultaneously detecting a plurality of indexes at one time.
(3) The utility model constructs a multi-layer ink dam on the surface of the microelectrode to form a concentric circular electrolytic cell with a specific structure, thereby avoiding the mutual interference of different detection sites in the detection process and ensuring the sensitivity and the accuracy of the electrode.
(4) The utility model adopts the semiconductor technology to modify the pure metal reaction layer, especially the pure thick gold layer, on the surface of the microelectrode, ensures the sensitivity and accuracy of microelectrode detection, and can enable the specific working electrode to detect specific biochemical substances by modifying the specific reaction layer on the surface of the microelectrode and applying specific potential, thereby realizing the detection by pertinently selecting microelectrode integrated sensing electrodes with different module configurations according to specific detection requirements.
(5) The utility model sets a multi-layer structure on the surface of the microelectrode to prepare the all-solid-state ion selective electrode, and can select the microelectrode surface modification layer according to specific detection ions, thereby improving the accuracy and stability of the detection of the sensor, realizing the rapid detection of the ions and providing a new idea for the design and application of the multi-parameter portable integrated sensor.
Drawings
The utility model will be further described with reference to the accompanying drawings, in which embodiments do not constitute any limitation of the utility model, and other drawings can be obtained by one of ordinary skill in the art without inventive effort from the following drawings.
FIG. 1 is a schematic front view of an all-solid ion selective electrode;
FIG. 2 is a schematic back side view of an all-solid ion selective electrode;
FIG. 3 is a schematic front view of an all-solid-state ion-selective electrode circuit configuration;
FIG. 4 is a schematic rear view of an all-solid-state ion-selective electrode circuit configuration;
FIG. 5 is a cross-sectional view of a microelectrode in an all-solid ion-selective electrode;
FIG. 6 is a schematic diagram of the structure of a working electrode in an all-solid ion selective electrode;
fig. 7 is a schematic diagram of the structure of an external reference electrode in an all-solid ion selective electrode.
Legend description:
1. a first microelectrode; 2. a second microelectrode; 3. a third microelectrode; 4. a fourth microelectrode; 5. a fifth microelectrode; 6. a first conduction point; 7. a second conduction point; 8. a third conduction point; 9. a fourth conduction point; 10. fifth conduction point position; 11. a first electrode contact site; 12. a second electrode contact site; 13. a third electrode contact site; 14. a fourth electrode contact site; 15. a fifth electrode contact site; 16. positioning holes; 17. a first layer of ink; 18. a second layer of ink; 19. a third layer of ink; 20. a first layer electrolytic cell; 21. a second layer electrolytic cell; 22. a third layer of electrolytic cell; 23. an internal reference electrode; 24. a base electrode; 25. conducting polymer gel; 26. an ion-selective permeable membrane; 27. a silver chloride electrode; 28. a base electrode; 29. PVC protective film.
Detailed Description
The utility model will be further described in detail with reference to the following specific examples, with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the utility model more apparent.
Example 1
As shown in figure 1, the front surface of the electrode is provided with five detection points, five circular microelectrodes are respectively arranged, the electrode comprises a first microelectrode 1 serving as an external reference electrode, a second microelectrode 2 serving as an auxiliary electrode, a third microelectrode 3 serving as a working electrode, a fourth microelectrode 4 and a fifth microelectrode 5, wherein the fifth microelectrode 5 is a calibration electrode.
As shown in fig. 3, the first microelectrode 1 is connected to the first conduction point 6 through a metal line, the second microelectrode 2 is connected to the second conduction point 7 through a metal line, the third microelectrode 3 is connected to the third conduction point 8 through a metal line, the fourth microelectrode 4 is connected to the fourth conduction point 9 through a metal line, and the fifth microelectrode 5 is connected to the fifth conduction point 10 through a metal line.
As shown in fig. 2 and fig. 4, the back surface of the electrode is provided with five rectangular electrode contact sites, including a first electrode contact site 11, a second electrode contact site 12, a third electrode contact site 13, a fourth electrode contact site 14 and a fifth electrode contact site 15, the first electrode contact site 11 is connected to the first conduction point 6 through a metal line, the second electrode contact site 12 is connected to the second conduction point 7 through a metal line, the third electrode contact site 13 is connected to the third conduction point 8 through a metal line, the fourth electrode contact site 14 is connected to the fourth conduction point 9 through a metal line, and the fifth electrode contact site 15 is connected to the fifth conduction point 10 through a metal line.
The first microelectrode 1 is connected with the first electrode contact site 11 through the first conduction point 6 in a conduction way, the second microelectrode 2 is connected with the second electrode contact site 12 through the second conduction point 7 in a conduction way, the third microelectrode 3 is connected with the third electrode contact site 13 through the third conduction point 8 in a conduction way, the fourth microelectrode 4 is connected with the fourth electrode contact site 14 through the fourth conduction point 9 in a conduction way, and the fifth microelectrode 5 is connected with the fifth electrode contact site 15 through the fifth conduction point 10 in a conduction way.
As shown in fig. 7, the first microelectrode 1 is an external reference electrode, the base electrode (28) is a pure gold electrode, gold is used as a basal layer, the diameter of the electrode is 0.8mm, the surface of the electrode is covered with a silver chloride electrode (27), the diameter of the electrode is 0.6mm, the outermost layer is modified with a PVC protective film (29), and the diameter of the PVC protective film (29) is 1.7mm.
The second microelectrode 2 is an auxiliary electrode and adopts a platinum electrode.
The third microelectrode 3 and the fourth microelectrode 4 are working electrodes which comprise a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion-selective permeable membrane (26) as shown in FIG. 6. The base electrode (24) is specifically a gold electrode, the diameter of the electrode is 0.8mm, an internal reference electrode (23), specifically a silver chloride electrode, the diameter of the electrode is 0.6mm, then the conductive polymer gel (25) is modified, the diameter of the conductive polymer gel (25) is 1.2mm, the surface of the conductive polymer gel is covered with an ion selective permeable membrane (26), and the diameter of the ion selective permeable membrane (26) is 1.5mm.
The surfaces of five microelectrodes arranged on the front face of the all-solid-state ion selective electrode are provided with a multi-layer electrolytic cell, as shown in figure 5, the diameter of a microelectrode metal modification layer is 1.20mm, a first layer of ink 17 is modified on the electrode surface, the electrode surface area which is not covered by the electrode modification layer and the electrode edge part area are mainly covered, and the electrode layer which is not covered by the ink and an ink surrounding dam form a first layer electrolytic cell 20, and the diameter of the first layer of electrolytic cell is 0.90mm; the surface of the first layer of ink 17 is continuously decorated with the second layer of ink 18, and a dam thereof forms a second layer of electrolytic cell 21 with the diameter of 1.10mm on the basis of the first layer of electrolytic cell 20; the surface of the second layer of ink 18 is further modified with a third layer of ink 19, a surrounding dam of the third layer of ink 19 forms a third layer of electrolytic cell 22 with the diameter of 1.30mm on the basis of the first layer of electrolytic cell 20 and the second layer of electrolytic cell 21, and the third layer of electrolytic cell presents a concentric circular structure. The number of layers of the electrolytic cell can be increased or decreased according to actual detection requirements.
The electrodes are provided with positioning holes 16, in particular in the upper left and upper right corners of the electrodes.
Example 2
As shown in figure 1, the front surface of the electrode is provided with five detection points, five circular microelectrodes are respectively arranged, the electrode comprises a first microelectrode 1 serving as an external reference electrode, a second microelectrode 2 serving as an auxiliary electrode, a third microelectrode 3 serving as a working electrode, a fourth microelectrode 4 and a fifth microelectrode 5, wherein the fifth microelectrode 5 is a calibration electrode.
As shown in fig. 3, the first microelectrode 1 is connected to the first conduction point 6 through a metal line, the second microelectrode 2 is connected to the second conduction point 7 through a metal line, the third microelectrode 3 is connected to the third conduction point 8 through a metal line, the fourth microelectrode 4 is connected to the fourth conduction point 9 through a metal line, and the fifth microelectrode 5 is connected to the fifth conduction point 10 through a metal line.
As shown in fig. 2 and fig. 4, the back surface of the electrode is provided with five rectangular electrode contact sites, including a first electrode contact site 11, a second electrode contact site 12, a third electrode contact site 13, a fourth electrode contact site 14 and a fifth electrode contact site 15, the first electrode contact site 11 is connected to the first conduction point 6 through a metal line, the second electrode contact site 12 is connected to the second conduction point 7 through a metal line, the third electrode contact site 13 is connected to the third conduction point 8 through a metal line, the fourth electrode contact site 14 is connected to the fourth conduction point 9 through a metal line, and the fifth electrode contact site 15 is connected to the fifth conduction point 10 through a metal line.
The first microelectrode 1 is connected with the first electrode contact site 11 through the first conduction point 6 in a conduction way, the second microelectrode 2 is connected with the second electrode contact site 12 through the second conduction point 7 in a conduction way, the third microelectrode 3 is connected with the third electrode contact site 13 through the third conduction point 8 in a conduction way, the fourth microelectrode 4 is connected with the fourth electrode contact site 14 through the fourth conduction point 9 in a conduction way, and the fifth microelectrode 5 is connected with the fifth electrode contact site 15 through the fifth conduction point 10 in a conduction way.
As shown in fig. 7, the first microelectrode 1 is an external reference electrode, the base electrode (28) is a pure gold electrode, gold is used as a basal layer, the diameter of the electrode is 0.8mm, the surface of the electrode is covered with a silver chloride electrode (27), the diameter of the electrode is 0.6mm, the outermost layer is modified with a PVC protective film (29), and the diameter of the PVC protective film (29) is 1.7mm.
The second microelectrode 2 is an auxiliary electrode and adopts a platinum electrode.
The third microelectrode 3 and the fourth microelectrode 4 are working electrodes which comprise a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion-selective permeable membrane (26) as shown in FIG. 6. The base electrode (24) is specifically a gold electrode, the diameter of the electrode is 0.8mm, an internal reference electrode (23), specifically a silver chloride electrode, the diameter of the electrode is 0.6mm, then the conductive polymer gel (25) is modified, the diameter of the conductive polymer gel (25) is 1.2mm, the surface of the conductive polymer gel is covered with an ion selective permeable membrane (26), and the diameter of the ion selective permeable membrane (26) is 1.5mm.
The surfaces of five microelectrodes arranged on the front face of the all-solid-state ion selective electrode are provided with a multi-layer electrolytic cell, as shown in figure 5, the diameter of a microelectrode metal modification layer is 0.40mm, a first layer of ink 17 is modified on the electrode surface, the electrode surface area which is not covered by the electrode modification layer and the electrode edge part area are mainly covered, and the electrode layer which is not covered by the ink and an ink surrounding dam form a first layer electrolytic cell 20, and the diameter of the first layer of electrolytic cell is 0.20mm; the surface of the first layer of ink 17 is continuously decorated with the second layer of ink 18, and a dam thereof forms a second layer of electrolytic cell 21 with the diameter of 0.30mm on the basis of the first layer of electrolytic cell 20; the surface of the second layer of ink 18 is further modified with a third layer of ink 19, a surrounding dam of the third layer of ink 19 forms a third layer of electrolytic cell 22 with the diameter of 0.50mm on the basis of the first layer of electrolytic cell 20 and the second layer of electrolytic cell 21, and the third layer of electrolytic cell presents a concentric circular structure. The number of layers of the electrolytic cell can be increased or decreased according to actual detection requirements.
The electrodes are provided with positioning holes 16, in particular in the upper left and upper right corners of the electrodes.
Example 3
As shown in figure 1, the front surface of the electrode is provided with five detection points, five circular microelectrodes are respectively arranged, the electrode comprises a first microelectrode 1 serving as an external reference electrode, a second microelectrode 2 serving as an auxiliary electrode, a third microelectrode 3 serving as a working electrode, a fourth microelectrode 4 and a fifth microelectrode 5, wherein the fifth microelectrode 5 is a calibration electrode.
As shown in fig. 3, the first microelectrode 1 is connected to the first conduction point 6 through a metal line, the second microelectrode 2 is connected to the second conduction point 7 through a metal line, the third microelectrode 3 is connected to the third conduction point 8 through a metal line, the fourth microelectrode 4 is connected to the fourth conduction point 9 through a metal line, and the fifth microelectrode 5 is connected to the fifth conduction point 10 through a metal line.
As shown in fig. 2 and fig. 4, the back surface of the electrode is provided with five rectangular electrode contact sites, including a first electrode contact site 11, a second electrode contact site 12, a third electrode contact site 13, a fourth electrode contact site 14 and a fifth electrode contact site 15, the first electrode contact site 11 is connected to the first conduction point 6 through a metal line, the second electrode contact site 12 is connected to the second conduction point 7 through a metal line, the third electrode contact site 13 is connected to the third conduction point 8 through a metal line, the fourth electrode contact site 14 is connected to the fourth conduction point 9 through a metal line, and the fifth electrode contact site 15 is connected to the fifth conduction point 10 through a metal line.
The first microelectrode 1 is connected with the first electrode contact site 11 through the first conduction point 6 in a conduction way, the second microelectrode 2 is connected with the second electrode contact site 12 through the second conduction point 7 in a conduction way, the third microelectrode 3 is connected with the third electrode contact site 13 through the third conduction point 8 in a conduction way, the fourth microelectrode 4 is connected with the fourth electrode contact site 14 through the fourth conduction point 9 in a conduction way, and the fifth microelectrode 5 is connected with the fifth electrode contact site 15 through the fifth conduction point 10 in a conduction way.
As shown in fig. 7, the first microelectrode 1 is an external reference electrode, the base electrode (28) is a pure gold electrode, gold is used as a basal layer, the diameter of the electrode is 0.8mm, the surface of the electrode is covered with a silver chloride electrode (27), the diameter of the electrode is 0.6mm, the outermost layer is modified with a PVC protective film (29), and the diameter of the PVC protective film (29) is 1.7mm.
The second microelectrode 2 is an auxiliary electrode and adopts a platinum electrode.
The third microelectrode 3 and the fourth microelectrode 4 are working electrodes which comprise a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion-selective permeable membrane (26) as shown in FIG. 6. The base electrode (24) is specifically a gold electrode, the diameter of the electrode is 0.8mm, an internal reference electrode (23), specifically a silver chloride electrode, the diameter of the electrode is 0.6mm, then the conductive polymer gel (25) is modified, the diameter of the conductive polymer gel (25) is 1.2mm, the surface of the conductive polymer gel is covered with an ion selective permeable membrane (26), and the diameter of the ion selective permeable membrane (26) is 1.5mm.
The surfaces of five microelectrodes arranged on the front face of the all-solid-state ion selective electrode are provided with a multi-layer electrolytic cell, as shown in figure 5, the diameter of a microelectrode metal modification layer is 1.40mm, a first layer of ink 17 is modified on the electrode surface, the electrode surface area which is not covered by the electrode modification layer and the electrode edge part area are mainly covered, and the electrode layer which is not covered by the ink and an ink surrounding dam form a first layer electrolytic cell 20, and the diameter of the first layer of electrolytic cell is 1.10mm; the surface of the first layer of ink 17 is continuously decorated with the second layer of ink 18, and a dam thereof forms a second layer of electrolytic cell 21 with the diameter of 1.20mm on the basis of the first layer of electrolytic cell 20; the surface of the second layer of ink 18 is further modified with a third layer of ink 19, a surrounding dam of the third layer of ink 19 forms a third layer of electrolytic cell 22 with the diameter of 1.50mm on the basis of the first layer of electrolytic cell 20 and the second layer of electrolytic cell 21, and the third layer of electrolytic cell presents a concentric circular structure. The number of layers of the electrolytic cell can be increased or decreased according to actual detection requirements.
The electrodes are provided with positioning holes 16, in particular in the upper left and upper right corners of the electrodes.
It will be evident to those skilled in the art that the utility model is not limited to the details of the foregoing illustrative embodiments, and that the present utility model may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the utility model being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined appropriately to form other embodiments that will be understood by those skilled in the art. Technical details not described in detail in the present utility model may be implemented by any prior art in the field. In particular, all technical features not described in detail in this utility model can be realized by any prior art technique.
Claims (8)
1. An all-solid-state ion selective electrode is characterized in that the front surface of the electrode is provided with five detection sites, and the five detection sites comprise a reference site, an auxiliary site, two detection sites and a calibration site; the detection site is provided with a corresponding microelectrode, the microelectrode comprises a working electrode, an external reference electrode and an auxiliary electrode, and the working electrode comprises a base electrode (24), an internal reference electrode (23), conductive polymer gel (25) and an ion selective permeable membrane (26); five electrode contact sites are arranged on the back of the electrode; the front and the back of the electrode are provided with five conducting sites conducting on two sides, and the detection sites and the electrode contact sites are connected to the conducting sites through metal lines and are correspondingly connected one by one.
2. The all-solid-state ion selective electrode according to claim 1, wherein the microelectrode surface is modified with a plurality of layers of ink to form an ink dam in the shape of concentric circles, the diameter of the concentric circles is 0.2-1.5 mm, and the center distance of the concentric circles is 20-150 μm.
3. An all-solid-state ion-selective electrode according to claim 2, wherein the ink dams are processed by using PCB solder resist ink, and the thickness of the ink dams is 20-50 μm.
4. An all-solid-state ion-selective electrode according to claim 1, characterized in that the electrode is provided with positioning holes (16), the positioning holes (16) being located in the upper left and upper right corners of the electrode.
5. An all-solid-state ion-selective electrode according to claim 1, characterized in that the surface of the base electrode (24) is modified with noble metals, including gold electrodes, platinum electrodes.
6. An all solid state ion selective electrode according to claim 1, characterized in that the internal reference electrode (23) comprises a silver chloride electrode.
7. An all solid state ion selective electrode according to claim 1, wherein the external reference electrode comprises a base electrode (28), a silver chloride electrode (27) and a PVC protective film (29).
8. An all-solid-state ion-selective electrode according to claim 7, characterized in that the surface of the base electrode (28) is modified with a noble metal electrode, including gold and platinum electrodes.
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