CN218885929U - Heart perfusion system for animal heart perfusion experiment - Google Patents
Heart perfusion system for animal heart perfusion experiment Download PDFInfo
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- CN218885929U CN218885929U CN202222752073.9U CN202222752073U CN218885929U CN 218885929 U CN218885929 U CN 218885929U CN 202222752073 U CN202222752073 U CN 202222752073U CN 218885929 U CN218885929 U CN 218885929U
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Abstract
The utility model discloses a heart perfusion system for animal heart perfusion experiments, which comprises an anti-drop heart perfusion needle, a fixed seat, an automatic liquid-changing perfusion pump, a liquid storage bottle and a waste liquid recovery diversion trench arranged at the periphery of an dissecting table and an dissecting table which are connected in sequence, wherein the front end of the anti-drop heart perfusion needle is provided with a needle point anti-drop sleeve, the rear end of the anti-drop heart perfusion needle is connected with a needle head tube body, the needle head tube body is provided with a rear positioning ring, and the tail end of the needle head tube body is connected with a needle cylinder connecting base connected with the fixed seat; the utility model discloses a reform transform the structure of perfusion needle, make the puncture ventricle that the perfusion needle can be quick and accurate to have certain anticreep and come out the fixity, through optimizing heart perfusion experimental system, increase devices such as automatic perfusion pump, reduce the degree of difficulty and the failure rate of animal heart perfusion experiment by a wide margin, improve the speed and the degree of automation that the experiment was gone on simultaneously by a wide margin, experiment operating personnel save time raises the efficiency.
Description
Technical Field
The utility model relates to a heart perfusion system for experiments, concretely relates to heart perfusion system for animal heart perfusion experiments.
Background
The animal heart perfusion experiment is an essential first step in the tissue drawing of animals, and the perfusion effect directly influences the state of tissues and is vital to the success of experiments such as tissue staining and the like;
the animal heart perfusion experiment firstly needs to expose an animal heart, a perfusion needle is inserted into a left ventricle of the animal, the perfusion needle is fixed, a right auricle is cut, and normal saline and paraformaldehyde are perfused in sequence, the difficulty in the experiment also can cause the experiment failure to be mainly that the perfusion needle is inserted into the left ventricle and is kept at the insertion position in the whole perfusion process, if the perfusion needle is excessively inserted, the ventricle is punctured, and the ventricle is separated from the ventricle after the perfusion, and the perfusion liquid can not enter the tissues of the whole body through a blood vessel circulation system of the animal under the two conditions, so that the experiment is irreversibly failed; in addition, in the experiment, the animal can have whole-body vibration due to formaldehyde perfusion, and the hydraulic pressure of perfusion liquid can generate reaction force and vibration on the perfusion needle, so that the steps are very tested on the experience and the manipulation of experimenters;
in addition, in perfusion, the animal needs to be perfused with normal saline or PBS to replace blood in a circulatory system and tissues, and then the perfusion liquid is replaced by paraformaldehyde, so as to fix the animal tissues; the step generally needs to quantitatively introduce a first perfusion solution, namely physiological saline or PBS, seamlessly, namely, the liquid is kept in a continuous bubble-free state, and a second perfusion solution, namely paraformaldehyde, is replaced and quantitatively introduced, so that the fixation of the whole body tissues of the animal is completed, wherein the step generally needs manual operation and whole-process monitoring of experimenters;
at present, no special animal heart perfusion device exists in the market, most laboratory personnel adopt a perfusion system which is assembled by self, for a perfusion needle, a common injection or transfusion needle and a hose are mostly adopted, and the perfusion needle is fixed by using vascular forceps or directly sticking and fixing the perfusion needle by an adhesive tape after being inserted into the heart; the adoption of a common needle for injection or transfusion has the following defects that can not be overcome:
firstly, because the ventricle needs to be penetrated smoothly, a sharp needle head is needed, but the requirement for grasping the needle inserting distance of an experimenter is high, and the experiment fails once too much or insufficient needle inserting is performed;
secondly, the blood vessel forceps or the adhesive tape is needed for fixation, and small animals with small hearts, such as mice, young rats and the like, are difficult to succeed at one time, usually need multiple attempts, not only waste time, but also possibly cause the perfusion needle to be separated due to operation;
thirdly, the blood vessel forceps are fixed by directly clamping the ventricle and the perfusion needle, which is easy to cause irreversible mechanical damage to heart tissues and cause perfusion liquid to flow out;
for the power of perfusion, a peristaltic pump is generally directly adopted, or a gravity perfusion mode such as a medical hanging bottle is adopted, and the perfusion mode generally needs manual liquid replacement, so that the attention and time of experimenters are consumed, and bubbles are easily introduced into a perfusion tube in the perfusion liquid replacement operation, so that perfusion liquid is blocked from flowing through a circulating system to enter tissues.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is because the perfusion needle deviates from or the transition pierces the perfusion failure problem that leads to in the animal heart perfusion experiment, the utility model provides a heart perfusion system for animal heart perfusion experiment can stabilize quick fixed perfusion pipe, and then has prevented deviating from of perfusion in-process perfusion needle, adopts the perfusion pump that can carry out automatic quick liquid changing, has simplified the liquid changing operation in the perfusion experiment and has solved the problem that produces the bubble in the manual work liquid changing operation for solve the defect that prior art leads to.
In order to solve the technical problem, the utility model provides a following technical scheme:
the first aspect, a heart perfusion system for animal heart perfusion experiment, wherein, contain anticreep heart perfusion needle, fixing base, the automatic perfusion pump, the stock solution bottle of trading that connect gradually to and dissect platform and dissect the peripheral waste liquid recovery guiding gutter that sets up of platform, anticreep heart perfusion needle front end is equipped with needle point anticreep sleeve pipe, connects the syringe needle body thereafter, and be equipped with the rear holding ring on the syringe needle body, syringe needle body trailing end connection have with the connection base that the fixing base is connected.
The heart perfusion system for the animal heart perfusion experiment is characterized in that a universal pipe is installed at the top of the fixing base, a three-way pipe is installed in the fixing base, one end of the three-way pipe is connected with a perfusion pipe, after the perfusion pipe penetrates through the universal pipe, part of the perfusion pipe extends out of the universal pipe and is connected with the needle cylinder connecting base, and the other two ends of the three-way pipe are respectively communicated with the two liquid storage bottles after being connected with the two dominant pump heads of the automatic liquid changing perfusion pump through a liquid inlet pipe.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the needle cylinder connecting base is a standard luer connector.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the length of the needle tube body is 10-100mm, the outer diameter of the needle tube body is 0.4-1.5mm, and the outer diameter of the needle tube body is consistent with the inner diameter of the needle-point anti-falling sleeve.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the front end of the needle point anti-falling sleeve is a 15-30-degree beveled needle point, the tail end of the needle point anti-falling sleeve is a 15-30-degree beveled barb, the length of the needle point anti-falling sleeve is 1-2mm, the outer diameter of the needle point anti-falling sleeve is 0.8-2mm, and the inner diameter of the needle point anti-falling sleeve is 0.4-1.5mm.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the distance between the rear positioning ring and the front end of the needle tube body is 3-7mm, and the distance between the rear positioning ring and the tail end of the needle point anti-falling sleeve is 1-3mm.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the outer diameter of the rear positioning ring is 2-4mm, and the inner diameter of the rear positioning ring is consistent with the outer diameter of the needle tube body.
In the heart perfusion system for animal heart perfusion experiments, the rear positioning ring is a circular ring made of metal or plastic.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the needle tube body and the needle point anti-drop sleeve are both made of medical stainless steel, and the needle tube connecting base is made of medical plastic or medical stainless steel.
In a second aspect, a method for using a heart perfusion system for an animal heart perfusion experiment, comprises the following steps:
step 1: fixing the animal on an anatomical table with the ventral side upward after anesthesia, opening the chest cavity by an operation, and exposing the heart;
step 2: adjusting the shape of a universal tube on the fixing seat to enable the needle cylinder connecting base to be approximately positioned at the position 3-10cm beside the heart of the animal;
and step 3: the anti-drop heart perfusion needle is directly used for puncturing the left ventricle of an animal, and the inverted hook behind the needle point anti-drop sleeve can prevent the punctured anti-drop heart perfusion needle from dropping out of the left ventricle;
and 4, step 4: cutting the right auricle of the animal, wherein in the process of perfusion, the blood and perfusion liquid of the animal flow out from the cut opening after passing through the circulatory system of the animal;
and 5: and starting the automatic liquid changing perfusion pump, pumping the first perfusion liquid into the left ventricle of the animal and perfusing the first perfusion liquid into the whole animal body, then automatically changing the next perfusion liquid by the automatic liquid changing perfusion pump to enter the whole animal body and automatically stopping the operation according to the set time or perfusion volume, and finishing the perfusion experiment.
The method for using the heart perfusion system for the animal heart perfusion experiment comprises the following steps of (1) enabling the animal to be a small animal;
in the step 3, the puncture depth of the anti-drop heart perfusion needle is more than the length of the needle point anti-drop sleeve.
According to above-mentioned the utility model relates to a technical scheme that animal heart perfusion experiment heart perfusion system provided has following technological effect:
the anti-drop heart perfusion needle provided with the needle tip anti-drop sleeve, the needle head tube body and the rear positioning ring can keep fixed in position after effectively penetrating into a ventricle, has certain traction resistance and is not easy to drop; the perfusion tube can be flexibly fixed by adopting the fixing seat with the universal tube, so that the falling-off prevention heart perfusion needle is further prevented from falling off in the perfusion process, and the fixation of the falling-off prevention heart perfusion needle is assisted; the automatic and rapid liquid changing perfusion pump is adopted, so that the liquid changing operation in a perfusion experiment is simplified, and the problem of bubbles generated in manual liquid changing operation is solved; the failure rate of the heart perfusion experiment can be greatly reduced, the occupation of the experiment on the personnel time is greatly improved, and the experiment efficiency is greatly improved.
Drawings
Fig. 1 is a schematic structural diagram of a heart perfusion system for animal heart perfusion experiments according to the present invention;
fig. 2 is a schematic structural view of an anti-drop heart perfusion needle in a heart perfusion system for animal heart perfusion experiments according to the present invention;
fig. 3 is a schematic structural diagram of a fixing base in a heart perfusion system for animal heart perfusion experiments.
Wherein the reference numbers are as follows:
1. a heart perfusion needle for preventing dropping; 2. a fixed seat; 3. automatic liquid changing perfusion pump; 4. a liquid storage bottle; 5. an dissecting table; 6. a waste liquid recovery diversion trench; 11. a needle tip anti-drop sleeve; 12. a needle body; 13. a rear positioning ring; 14. the needle cylinder is connected with the base; 21. a three-way pipe; 22. a universal tube.
Detailed Description
In order to make the technical means, the inventive features, the objectives, and the functions of the present invention easy to understand and understand, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.
Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve.
In addition, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are used for clarity of description only, and are not used to limit the scope of the present invention, and the relative relationship between the terms may be changed or adjusted without substantial technical changes.
The utility model discloses a first embodiment provides an animal heart perfusion experiment is with heart perfusion system, the utility model discloses a second embodiment provides an animal heart perfusion experiment is with heart perfusion system and application method thereof, and the purpose adopts the perfusion pump that can carry out automatic quick liquid changing, has simplified the liquid changing operation in the perfusion experiment and has solved the problem that produces the bubble in the artifical liquid changing operation.
As shown in fig. 1-2, in a first aspect, a first embodiment, a heart perfusion system for animal heart perfusion experiments, includes an anti-drop heart perfusion needle 1, a fixing base 2, an automatic liquid-changing perfusion pump 3, a liquid storage bottle 4, and a waste liquid recovery guiding groove 6 disposed at the periphery of an dissecting table 5 and the dissecting table 5, which are connected in sequence, wherein a needle point anti-drop casing 11 is disposed at the front end of the anti-drop heart perfusion needle 1, a needle tube 12 is connected behind the needle tube 12, a rear positioning ring 13 is disposed on the needle tube 12, and a syringe connecting base 14 connected to the fixing base 2 is connected to the rear end of the needle tube 12.
As shown in fig. 3, in the heart perfusion system for animal heart perfusion experiments, a universal pipe 22 is installed at the top of a fixed seat 2, a three-way pipe 21 is installed in the fixed seat 2, one end of the three-way pipe is connected with a perfusion pipe, after the perfusion pipe penetrates through the universal pipe 22, part of the perfusion pipe extends out of the universal pipe 22 and is connected with a needle cylinder connecting base 14, and the other two ends of the three-way pipe 21 are connected with two dominant pump heads of an automatic liquid changing perfusion pump 3 through a liquid inlet pipe and then are respectively communicated with two liquid storage bottles 4;
the automatic liquid changing perfusion pump 3 uses two peristaltic pump heads, the two pump heads can be respectively used for speed regulation and pump feeding direction setting, the two pump heads alternately run, namely, when one pump head is started, the other pump head is closed, when the first pump head is closed, the second pump head is automatically started, and the automatic liquid changing perfusion pump 3 has a timing cycle switch function.
In the heart perfusion system for the animal heart perfusion experiment, the needle cylinder connecting base 14 is a standard luer connector, medical plastic or medical stainless steel is used, and the heart perfusion system is similar to a standard syringe needle base and can be directly connected with a 3.2mm luer connector of a common needle cylinder and a perfusion tube.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the length of the needle tube body 12 is 10-100mm, the outer diameter is 0.4-1.5mm, the outer diameter is consistent with the inner diameter of the needle point anti-falling sleeve tube 11, the needle tube body 12 is made of medical stainless steel, the structure of the needle tube body is similar to that of a common needle tube, and the needle tube body needs to be consistent with the inner diameter of the needle point anti-falling sleeve tube 11 according to the use scene, namely the size of the animal heart and the thickness of the ventricular wall.
The heart perfusion system for the animal heart perfusion experiment comprises a needle point anti-falling sleeve 11, wherein the front end of the needle point anti-falling sleeve is a 15-30-degree beveled needle point, the tail end of the needle point anti-falling sleeve is a 15-30-degree beveled barb, the length of the needle point anti-falling sleeve 11 is 1-2mm, the outer diameter of the needle point anti-falling sleeve 11 is 0.8-2mm, the inner diameter of the needle point anti-falling sleeve is 0.4-1.5mm, the needle point anti-falling sleeve 11 is made of medical stainless steel, the tip end and the edge of the beveled needle point of the needle point anti-falling sleeve 11 are sharp according to the use scene, namely the size of an animal heart and the thickness of a ventricle wall, tissue can be easily punctured, the beveled barb of the needle point anti-falling sleeve 11 is consistent with the cutting angle of the beveled needle point, sharp treatment is not performed, and the barb of the anti-falling heart perfusion needle 1 can be fixed on the inner wall of the ventricle while the tissue is not punctured.
The heart perfusion system for the animal heart perfusion experiment is characterized in that the distance between the rear positioning ring 13 and the front end of the needle tube body 12 is 3-7mm, the distance between the rear positioning ring 13 and the tail end of the needle point anti-falling sleeve tube 11 is 1-3mm, the rear positioning ring 13 is a metal or plastic circular ring, the outer diameter of the rear positioning ring is 2-4mm, the rear positioning ring is larger than the outer diameter of the needle point anti-falling sleeve tube 11, the inner diameter of the rear positioning ring is consistent with the outer diameter of the needle tube body 12, the rear positioning ring is fixed on the needle tube body 12 and is positioned at the rear end of the needle point anti-falling sleeve tube 12 by 1-3mm (the distance is slightly larger than the wall of the heart chamber according to the wall of the heart inserted into the animal heart).
In a second aspect, a second embodiment, a method for using a heart perfusion system for an animal heart perfusion experiment, comprises the following steps:
step 1: after the animal is anesthetized, the animal is fixed on an dissecting table 5 with the ventral side upward, and the thoracic cavity is opened by operation to expose the heart;
step 2: the shape of the universal tube 22 on the fixed seat 2 is adjusted, so that the needle cylinder connecting base 14 is approximately positioned 3-10cm beside the heart of the animal;
and 3, step 3: the anti-dropping heart perfusion needle 1 is directly punctured into the left ventricle of the animal, and the reverse hook at the rear part of the needle point anti-dropping sleeve 11 can prevent the punctured anti-dropping heart perfusion needle from dropping out of the left ventricle;
and 4, step 4: cutting the right auricle of the animal, wherein in the process of perfusion, the blood and perfusion liquid of the animal flow out from the cut opening after passing through the circulatory system of the animal;
and 5: and starting the automatic liquid changing perfusion pump 3, pumping the first perfusion liquid into the left ventricle of the animal and perfusing the first perfusion liquid into the whole animal body, then automatically changing the next perfusion liquid by the automatic liquid changing perfusion pump 3 to enter the whole animal body and automatically stopping the operation according to the set time or perfusion volume, and finishing the perfusion experiment.
The method for using the heart perfusion system for the animal heart perfusion experiment comprises the following steps of (1) taking small animals as animals, preferably rats or mice;
in the step 3, the puncture depth of the anti-falling heart perfusion needle 1 is more than the length of the needle point anti-falling sleeve 11.
To sum up, the utility model discloses a heart perfusion system for animal heart perfusion experiments adopts the perfusion pump that can carry out automatic liquid of trading fast, has simplified the liquid operation of trading in the perfusion experiment and has solved the problem that produces the bubble in the manual work liquid operation of trading.
The above description has been made of specific embodiments of the present invention. It is to be understood that the invention is not limited to the specific embodiments described above, and that equipment and structures not described in detail are understood to be practiced in a manner that is conventional in the art; various changes or modifications may be made by those skilled in the art without departing from the scope of the claims, and the scope of the invention is not to be limited to the embodiments described in the claims.
Claims (9)
1. The utility model provides an animal heart perfusion experiment is with heart perfusion system, its characterized in that contains anticreep heart perfusion needle (1), fixing base (2), automatic perfusion pump (3), stock solution bottle (4) of trading that connect gradually to and dissection platform (5) and the peripheral waste liquid recovery guiding gutter (6) that sets up of dissection platform (5), anticreep heart perfusion needle (1) front end is equipped with needle point anticreep sleeve pipe (11), connects syringe needle body (12) thereafter, and be equipped with rear holding ring (13) on syringe needle body (12), syringe needle body (12) trailing end connection have with base (14) is connected to the cylinder that fixing base (2) are connected.
2. The heart perfusion system for the animal heart perfusion experiment as recited in claim 1, wherein a universal tube (22) is installed at the top of the fixing base (2), a three-way tube (21) is installed in the fixing base (2), one end of the three-way tube is connected with a perfusion tube, after the perfusion tube penetrates through the universal tube (22), a part of the perfusion tube protrudes out of the universal tube (22) and is connected with the needle cylinder connecting base (14), and the other two ends of the three-way tube (21) are connected with two perfusion pump heads of the automatic liquid changing pump (3) through liquid inlet tubes and are respectively communicated with the two liquid storage bottles (4).
3. A heart perfusion system for animal heart perfusion experiments according to claim 2, wherein the syringe connection base (14) is a standard luer connector.
4. A heart perfusion system for animal heart perfusion experiment according to any one of claims 1-3, characterized in that the needle tube body (12) has a length of 10-100mm and an outer diameter of 0.4-1.5mm, and the outer diameter is consistent with the inner diameter of the needle tip anti-drop sleeve (11).
5. The heart perfusion system for animal heart perfusion experiments as claimed in claim 4, wherein the front end of the needle point anti-drop sleeve (11) is a beveled needle point with an angle of 15-30 degrees, the tail end of the needle point anti-drop sleeve is a beveled barb with an angle of 15-30 degrees, the length of the needle point anti-drop sleeve (11) is 1-2mm, the outer diameter is 0.8-2mm, and the inner diameter is 0.4-1.5mm.
6. The heart perfusion system for animal heart perfusion experiments as claimed in claim 5, wherein the distance between the rear positioning ring (13) and the front end of the needle tube body (12) is 3-7mm, and the distance between the rear positioning ring and the tail end of the needle point anti-drop sleeve (11) is 1-3mm.
7. A heart perfusion system for animal heart perfusion experiments according to claim 6, wherein the rear positioning ring (13) has an outer diameter of 2-4mm and an inner diameter which is consistent with the outer diameter of the needle tube body (12).
8. A heart perfusion system for animal heart perfusion experiments according to claim 7, wherein the rear positioning ring (13) is a metal or plastic ring.
9. A heart perfusion system for animal heart perfusion experiment according to any one of claims 1-3, characterized in that the needle tube body (12) and the needle point anti-drop sleeve (11) are made of medical stainless steel material, and the syringe connecting base (14) is made of medical plastic or medical stainless steel material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222752073.9U CN218885929U (en) | 2022-10-19 | 2022-10-19 | Heart perfusion system for animal heart perfusion experiment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222752073.9U CN218885929U (en) | 2022-10-19 | 2022-10-19 | Heart perfusion system for animal heart perfusion experiment |
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| Publication Number | Publication Date |
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| CN218885929U true CN218885929U (en) | 2023-04-18 |
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| CN202222752073.9U Active CN218885929U (en) | 2022-10-19 | 2022-10-19 | Heart perfusion system for animal heart perfusion experiment |
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- 2022-10-19 CN CN202222752073.9U patent/CN218885929U/en active Active
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