CN218879933U

CN218879933U - Automatic stop device and biological reaction equipment for conveying cells and culture medium

Info

Publication number: CN218879933U
Application number: CN202223412637.0U
Authority: CN
Inventors: 农焕子; 张程; 俞世清
Original assignee: Shenzhen Ruisheng Bioengineering Co ltd
Current assignee: Shenzhen Ruisheng Bioengineering Co ltd
Priority date: 2022-12-15
Filing date: 2022-12-15
Publication date: 2023-04-18
Anticipated expiration: 2032-12-15

Abstract

The utility model discloses an automatic cut-off device and biological reaction equipment of transport of cell and culture medium, wherein, the automatic cut-off device of transport of cell and culture medium includes: the device comprises a culture device, a reactor and a conveying pipe, wherein one end of the conveying pipe extends into the culture device, and the other end of the conveying pipe extends into the reactor; the flow sensor, the control valve and the delivery pump are all arranged on the delivery pipe; and the controller is electrically connected with the flow sensor, the control valve and the delivery pump. The technical proposal of the utility model aims at reducing the occurrence probability of the cell sap in the air pollution reactor.

Description

Automatic stop device and biological reaction equipment for conveying cells and culture medium

Technical Field

The utility model relates to a biological reaction equipment technical field, in particular to automatic stop device and biological reaction equipment of carrying of cell and culture medium.

Background

In the technical field of biological reaction, as the cell sap itself is susceptible to the external environment, care needs to be taken during the transportation of the cell sap to prevent the interior of the cell sap from being polluted due to air entering the cell sap, most of the cell sap is transported into a reactor from an incubator through a transport pipe, and it needs to be noted that most of the cell sap is quantitatively transported during the transportation, that is, the amount of the cell sap transported into the reactor from the incubator is fixed every time, however, in some cases, the amount of the cell sap in the incubator does not reach the specified standard of quantitative transportation, and at this time, the transport pipe is likely to work after the cell sap in the incubator is transported, so that the air in the incubator is easily sucked into the cell sap in the reactor, and further the cell sap in the reactor is polluted.

SUMMERY OF THE UTILITY MODEL

The utility model mainly aims to provide an automatic stop device for conveying cells and culture medium, which aims to reduce the probability of occurrence of cell sap in an air pollution reactor.

In order to achieve the above object, the present invention provides an automatic stop device for conveying cells and culture medium, comprising: the device comprises a culture device, a reactor and a conveying pipe, wherein one end of the conveying pipe extends into the culture device, and the other end of the conveying pipe extends into the reactor;

the flow sensor, the control valve and the delivery pump are all arranged on the delivery pipe; and

and the controller is electrically connected with the flow sensor, the control valve and the delivery pump.

Optionally, the transfer pump is disposed proximate to the incubator.

Optionally, the delivery pump comprises a peristaltic pump and a pump motor for driving the peristaltic pump, and the controller is electrically connected to the pump motor.

Optionally, the pump motor is configured as a stepper motor.

Optionally, the flow sensor is configured as a capacitive flow sensor.

Optionally, the control valve is configured as a pinch valve.

Optionally, the pinch valve is a V-shaped pinch valve.

Optionally, the automatic stopping device for the transportation of the cells and the culture medium further comprises a buzzer, and the buzzer is electrically connected to the controller.

Optionally, the automatic cut-off device for the delivery of the cells and the culture medium further comprises an emergency stop switch, and the emergency stop switch is electrically connected to the delivery pump.

The utility model also provides a biological reaction equipment, including the automatic device that ends of the transport of aforementioned cell and culture medium.

The utility model discloses the incubator among the technical scheme is used for cultivateing the cell sap that needs, and the reactor is used for collecting the cell sap of cultivateing in the incubator and supplies the user to carry out operation on next step, and wherein, the cell sap is transported through the conveyer pipe, and the controller comes the transport power as the cell sap in the conveyer pipe through controlling the delivery pump to the controller also can be through the transport condition of control valve control conveyer pipe in the conveyer pipe.

It should be noted that, in this technical solution, the flow sensor is used to detect the cell sap flow in the delivery pipe, and when the cell sap flow is lower than the rated value, the controller stops delivering the cell sap by controlling the closing of the control valve, and the device can realize automatic cut-off, thereby preventing the situation that the air in the incubator is sucked into the cell sap in the reactor due to the delivery pipe still delivering the cell sap when the cell sap is quantitatively delivered, and further reducing the occurrence probability of air pollution to the cell sap in the reactor.

Understandably, when the cell sap flow rate is lower than the rated value, the cell sap remained in the incubator is insufficient, and at this time, if the conveying pipe is still in a conveying state, air is conveyed into the reactor by the conveying pipe, and further, certain pollution can be caused to the cell sap in the reactor, therefore, the flow sensor can be set up to prevent the air from entering the cell sap in the reactor, and compared with a vacuum conveying mode, the technical scheme has the advantages of lower cost, simple user operation and better convenience, and correspondingly, the conveying efficiency of the cell sap can be better improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic view of an embodiment of the automatic stop device for transporting cells and culture medium according to the present invention.

The reference numbers indicate:

reference numerals	Name (R)	Reference numerals	Name (R)
				100	Culture device	200	Reactor with a reactor shell
300	Delivery pipe	400	Cell sap
				500	Flow sensor	510	Capacitive flow sensor
600	Control valve	610	Pinch valve
				700	Delivery pump	710	Peristaltic pump

The objects, features and advantages of the present invention will be further described with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that all the directional indicators (such as up, down, left, right, front, back ...) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a specific posture (as shown in the attached drawings), and if the specific posture is changed, the directional indicator is changed accordingly.

In the present application, unless expressly stated or limited otherwise, the terms "connected" and "fixed" are to be construed broadly, e.g., "fixed" may be fixedly connected or detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be interconnected within two elements or in a relationship where two elements interact with each other unless otherwise specifically limited. The specific meaning of the above terms in the present invention can be understood according to specific situations by those of ordinary skill in the art.

In addition, if there is a description relating to "first", "second", etc. in the embodiments of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the meaning of "and/or" appearing throughout includes three juxtapositions, exemplified by "A and/or B" including either A or B or both A and B. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

As shown in fig. 1:

the utility model provides an automatic stop device of transport of cell and culture medium.

In the embodiment of the present invention, the automatic stopping device for the cell and culture medium transportation comprises: the device comprises an incubator 100, a reactor 200 and a conveying pipe 300, wherein one end of the conveying pipe 300 extends into the incubator 100, and the other end of the conveying pipe 300 extends into the reactor 200;

the flow sensor 500, the control valve 600 and the delivery pump 700 are all arranged on the delivery pipe 300; and

a controller electrically connected to the flow sensor 500, the control valve 600, and the transfer pump 700.

The utility model discloses culture apparatus 100 among the technical scheme is used for cultivateing required cell sap 400, reactor 200 is used for collecting the cell sap 400 of cultivateing in culture apparatus 100 and supplies the user to carry out operation next step, wherein, cell sap 400 transports through conveyer pipe 300, the controller is through controlling delivery pump 700 as the power of carrying of cell sap 400 in conveyer pipe 300, and the controller also can be through the control valve 600 control the transport condition of conveyer pipe 300 in the conveyer pipe, the switch that the controller controlled the pump body and valve body according to the detection numerical value of feedback is prior art, it is no longer repeated here, correspondingly the device just can realize automatic ending, and then prevented cell sap 400 in the process of ration transportation, because of the volume of cell sap 400 in culture apparatus 100 does not reach the standard, conveyer pipe 300 is still transporting cell sap 400, and the condition in the cell sap 400 in the air that causes is inhaled reactor 200, the probability of occurrence of air pollution cell sap 400 has been reduced.

It should be noted that in the present embodiment, the flow sensor 500 is used to detect the flow rate of the cell fluid 400 in the delivery pipe 300, and when the flow rate of the cell fluid 400 is lower than the rated value, the controller stops the delivery of the cell fluid 400 by controlling the control valve 600 to close.

It can be understood that when the flow rate of the cell sap 400 is lower than the rated value, the cell sap 400 remained in the incubator 100 is insufficient, and if the delivery pipe 300 is still in the delivery state, air is delivered into the reactor 200 by the delivery pipe 300, which may cause certain pollution to the cell sap 400 in the reactor 200, therefore, the flow sensor 500 is set up to prevent air from entering the cell sap 400 in the reactor 200, compared with the vacuum delivery mode, the technical solution has lower cost, and the user operation is simple, the convenience is better, and accordingly, the delivery efficiency of the cell sap 400 can be better improved. Of course, in other embodiments, the cell liquid 400 in the incubator 100 may be introduced into the reactor 200 by gravity.

Further, the transfer pump 700 is disposed near the incubator 100. In this embodiment, the transfer pump 700 is disposed close to the incubator 100, that is, there is no other device between the transfer pump 700 and the incubator 100, and the transfer pump 700 is directly communicated with the inside of the incubator 100, so as to avoid the damage to the transfer pump 700 and other devices in the incubator 100 caused by the operation of the transfer pump 700 when the control valve 600 is in the valve-closed state. Of course, in other embodiments, other devices, such as flow sensor 500, may be added between the transfer pump 700 and the incubator 100.

Further, the delivery pump 700 includes a peristaltic pump 710 and a pump motor for driving the peristaltic pump 710, and the controller is electrically connected to the pump motor. In the present technical solution, the delivery pump 700 is a peristaltic pump 710 driven by a pump motor, the peristaltic pump 710 is set up such that the liquid in the delivery tube 300 only contacts the pump tube, but does not contact the pump body, thereby increasing the sterility of the cellular fluid 400, and the peristaltic pump 710 has a bidirectional delivery capability, such that when air enters the delivery tube 300, the peristaltic pump 710 can work reversely to discharge the air. In other embodiments, the delivery pump 700 may also be a hand pump.

Further, the pump motor is configured as a stepper motor. In this embodiment, the stepping motor is used as a pump motor of the peristaltic pump 710, and the stepping motor is set up, so that the control of the peristaltic pump 710 is more accurate, and the cost can be saved to a certain extent. Of course, in other embodiments, a servo motor may be used as the pump motor.

Further, the flow sensor 500 is configured as a capacitive flow sensor 510. In the present technical solution, the flow sensor 500 is a capacitive flow sensor 510, and it can be understood that the level in the capacitive flow sensor 510 changes with the change of the capacitance medium, and when the concentration of the cellular fluid 400 in the delivery pipe 300 changes, the medium changes with the change of the capacitance medium, which further causes the level to change, so as to achieve the function of detecting the flow of the cellular fluid 400, and the setting of the flow sensor 500 improves the accuracy of detection.

Further, the control valve 600 is configured as a pinch valve 610. In this embodiment, the pinch valve 610 serves as a control valve 600 for controlling the flow of the cell sap 400 in the delivery pipe 300, and the pinch valve 610 can either rapidly pinch the delivery pipe 300 to stop the delivery of the delivery pipe 300 or gradually reduce the diameter of the channel for delivering the cell sap 400 in the delivery pipe 300. It can be understood that, the pinch valve 610 is electrically connected with the flow sensor 500, when the flow sensor 500 detects that the delivery flow in the delivery pipe 300 is lower than the rated value, the pinch valve 610 gradually locks the delivery pipe 300, so that the cell fluid 400 in the delivery pipe 300 can always fill the delivery pipe 300, and finally, after the pinch valve 610 completely clamps the delivery pipe 300, the delivery pump 700 stops working, so that the cell fluid 400 in the incubator 100 can be sufficiently transported and prevented from being wasted.

Further, the pinch valve 610 is a V-shaped pinch valve 610. In the embodiment, the pinch valve 610 is V-shaped, and it can be understood that the V-shaped pinch valve 610 limits the shape of the conveying pipe 300, thereby enhancing the applicability of the pinch valve 610 and further improving the general applicability of the automatic stop device for conveying cells and culture medium.

Further, the automatic stopping device for conveying the cells and the culture medium further comprises a buzzer, and the buzzer is electrically connected to the controller. In this embodiment, a buzzer is connected to the controller, and when the flow sensor 500 detects that the flow rate of the cell fluid 400 in the delivery pipe 300 is lower than a rated value, the buzzer sounds, and the operator can replace the incubator 100 at the first time. In addition, in other embodiments, when the whole conveying automatic stopping device breaks down, the buzzer can also give an alarm, so that the maintenance can be carried out by workers at the first time, and more serious faults can be prevented.

Further, the automatic cut-off device for the delivery of the cells and the culture medium further comprises an emergency stop switch, and the emergency stop switch is electrically connected to the delivery pump 700. In this embodiment, the automatic conveying stopping device further includes an emergency stop switch, and it can be understood that the emergency stop switch is electrically connected to the conveying pump 700, and when the conveying pump 700 has a conveying failure, the whole device can be turned off only by pressing the emergency stop switch, so that a situation of an emergency situation is prevented, and the safety of the device is enhanced.

The utility model discloses still provide a biological reaction equipment, this biological reaction equipment include the automatic cut-off of transport of cell and culture medium, and the concrete structure of the automatic cut-off of transport of this aforementioned cell and culture medium refers to above-mentioned embodiment, because this biological reaction equipment has adopted all technical scheme of above-mentioned all embodiments, consequently has all beneficial effects that the technical scheme of above-mentioned embodiment brought at least, gives unnecessary detail here one by one.

The above is only the optional embodiment of the present invention, and not the scope of the present invention is limited thereby, all the equivalent structure changes made by the contents of the specification and the drawings are utilized under the inventive concept of the present invention, or the direct/indirect application in other related technical fields is included in the patent protection scope of the present invention.

Claims

1. An automatic cut-off device for conveying cells and culture media, which is characterized by comprising:

the device comprises a culture device, a reactor and a conveying pipe, wherein one end of the conveying pipe extends into the culture device, and the other end of the conveying pipe extends into the reactor;

2. The device for automatically shutting off the delivery of cells to a culture medium of claim 1, wherein said delivery pump is disposed adjacent to said incubator.

3. The automatic shut-off device for the delivery of cells and media according to claim 2, wherein the delivery pump comprises a peristaltic pump and a pump motor for driving the peristaltic pump, and the controller is electrically connected to the pump motor.

4. The device of claim 3, wherein the pump motor is configured as a stepper motor.

5. The device for automatically stopping delivery of cells and media according to claim 1, wherein said flow sensor is configured as a capacitive flow sensor.

6. The device of claim 1, wherein the control valve is configured as a pinch valve.

7. The automatic shut-off device for the delivery of cells and culture medium of claim 6, wherein the pinch valve is a V-shaped pinch valve.

8. The device for automatically stopping the delivery of cells and culture medium according to claim 1, further comprising a buzzer, wherein the buzzer is electrically connected to the controller.

9. The device for automatically stopping the delivery of cells and culture medium according to claim 1, further comprising an emergency stop switch electrically connected to the delivery pump.

10. A bioreactor apparatus comprising an automatic shut-off device for the delivery of cells and culture medium according to any one of claims 1 to 9.