CN218842171U - Liquid path monitoring device, gene sequencer and gene sequencing device - Google Patents
Liquid path monitoring device, gene sequencer and gene sequencing device Download PDFInfo
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- CN218842171U CN218842171U CN202223523561.9U CN202223523561U CN218842171U CN 218842171 U CN218842171 U CN 218842171U CN 202223523561 U CN202223523561 U CN 202223523561U CN 218842171 U CN218842171 U CN 218842171U
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Abstract
The present disclosure provides a liquid path monitoring device, gene sequencer and gene sequencing device, the liquid path monitoring device includes: the reagent selection module transmits the target reagent to the reaction chip through the liquid pipeline after receiving a reagent adding instruction sent by the controller; the reaction chip is used for treating a target reagent and transmitting the treated waste liquid to the pump set after flowing through the detection module through the liquid pipeline; the detection module is used for detecting the flow resistance of the flowing waste liquid and sending the flow resistance detection result to the controller; the controller is used for sending a pressure test instruction to the pump set and sending a liquid inlet stopping instruction to the reagent selection module and the pump set under the condition that the flow resistance detection is determined to be abnormal based on the flow resistance detection result; the pump set controls the reagent selection module to obtain the target reagent after receiving the reagent adding instruction sent by the controller; leading out waste liquid; and after receiving a pressure test instruction sent by the controller, the pressure air is transmitted to the reagent selection module so as to carry out pressure detection on the liquid pipeline through the detection module.
Description
Technical Field
The disclosure relates to the technical field of computers, in particular to a liquid path monitoring device, a gene sequencer and a gene sequencing device.
Background
At present, gene sequencing can analyze and determine the complete sequence of a gene, and is beneficial to predicting and preventing various diseases, so that the gene sequencing is widely applied. In the gene sequencing process, various reagents are required to be added to the reaction chip to complete the gene sequencing. In this process, if the fluid path system corresponding to gene sequencing fails, the gene sequencing result may be affected. Therefore, it is very important to detect the liquid path system in real time during the gene sequencing process.
SUMMERY OF THE UTILITY MODEL
The embodiment of the disclosure at least provides a liquid path monitoring device, a gene sequencer and a gene sequencing device.
In a first aspect, an embodiment of the present disclosure provides a liquid path monitoring device, including: the device comprises a reagent selection module, a reaction chip, a detection module, a pump set and a controller; the reagent selection module is used for transmitting a target reagent to the reaction chip through a liquid pipeline after receiving a reagent adding instruction sent by the controller; the reaction chip is used for processing the target reagent, and transmitting the processed waste liquid to the pump set after flowing through the detection module through a liquid pipeline; the detection module is used for detecting the flow resistance of the flowing waste liquid and sending the flow resistance detection result to the controller; the controller is used for sending a pressure test instruction to the pump set and sending a liquid inlet stopping instruction to the reagent selection module and the pump set under the condition that the flow resistance detection is determined to be abnormal based on the flow resistance detection result; the pump set is used for controlling the reagent selection module to obtain a target reagent after receiving a reagent adding instruction sent by the controller; leading out the waste liquid; and after receiving a pressure test instruction sent by the controller, transmitting pressure air to the reagent selection module so as to perform pressure detection on the liquid pipeline through the detection module.
In an alternative embodiment, the reagent selection module comprises at least one reagent selection valve; the reagent selection valve comprises a plurality of reagent selection ports for receiving different reagents and a common port; wherein the reagent selection port is for delivering the target reagent to the common port after receiving the target reagent; the common port is used for transmitting the received target reagent to the reaction chip through the liquid pipeline.
In an optional embodiment, the reaction chip comprises at least one liquid inlet, at least one liquid outlet corresponding to each liquid inlet, and at least one reaction channel, the liquid inlets and the liquid outlets corresponding to the liquid inlets are connected by the reaction channel, and the number of the liquid inlets is the same as that of the reagent selection valves; and the common port of the reagent selection valve is connected with the liquid inlet, and the liquid inlets connected with different reagent selection valves are different.
In an optional embodiment, the liquid outlets of the reaction chip are connected to the pump set, and when there are a plurality of liquid outlets corresponding to any liquid inlet, a liquid path pipeline connecting a target liquid outlet of the plurality of liquid outlets corresponding to the liquid inlet and the pump set is provided with the detection module.
In an alternative embodiment, the pump stack includes a pump stack selection valve including a plurality of ports; the liquid outlet of the reaction chip is respectively connected with a first port of the pump set selection valve, and the first port is used for conveying the waste liquid; the second port of the pump group selection valve is connected with the reagent selection port of the reagent selection valve, and the second port is used for conveying pressure air during pressure test; and the pump set is also used for controlling the communication of a target port corresponding to the target instruction in a plurality of ports of the pump set selection valve after receiving the target instruction sent by the controller, wherein the target instruction comprises the reagent adding instruction and the pressure testing instruction.
In an alternative embodiment, the pump set further comprises a pressure control module in communication with the pump set selection valve for controlling the pressure in the fluid line conduit.
In an optional implementation, the detection module is further configured to: sending a pressure test result to the controller; the controller is further configured to: and controlling a display module to display pressure abnormity information under the condition that the pressure test result is detected to be abnormal.
In an optional embodiment, the liquid path monitoring device further includes a waste liquid barrel, and the waste liquid barrel is connected to the pump set through a liquid path pipeline and used for storing the waste liquid.
In a second aspect, embodiments of the present disclosure also provide a gene sequencer, including: the fluid path monitoring device according to any one of the first to third aspects.
In a third aspect, embodiments of the present disclosure further provide a gene sequencing apparatus, including: a housing and a gene sequencer as described in the second aspect above.
The liquid path monitoring device provided by the embodiment of the disclosure can comprise a reagent selection module, a reaction chip, a detection module, a pump set and a controller. The detection module can be arranged in a liquid pipeline between the reaction chip and the pump set, so that the flow resistance of flowing waste liquid can be detected through the detection module, and the flow resistance detection result is sent to the controller. Then, in a case where it is determined that the flow resistance detection is abnormal based on the flow resistance detection result, a pressure detection instruction may be sent to the pump group to perform a pressure test on the liquid path piping of the liquid path monitoring apparatus.
In the above embodiment, the detection module may be disposed in the fluid path pipeline between the reaction chip and the pump set, so as to monitor the flow resistance information in the fluid path pipeline in real time, thereby implementing real-time monitoring of the fluid path monitoring device, and determining the health status of the fluid path monitoring device in real time. And after the pressure detection instruction is received, pressure air can be transmitted to the reagent selection module based on the pump to carry out pressure test on the liquid pipeline through the detection module under the condition that the existing structure of the liquid pipeline system is not changed, so that manpower and material resources can be saved, and the cost is reduced.
In order to make the aforementioned objects, features and advantages of the present disclosure more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings needed to be used in the embodiments will be briefly described below. It is to be understood that the following drawings depict only certain embodiments of the disclosure and are therefore not to be considered limiting of its scope, for those skilled in the art to which the disclosure pertains without the benefit of the inventive faculty, and that additional related drawings may be derived therefrom.
Fig. 1 is a schematic structural diagram of a related art liquid path system provided in an embodiment of the present disclosure;
FIG. 2 is a schematic structural diagram illustrating a flow resistance detection of a fluid path system in the related art according to an embodiment of the disclosure;
fig. 3 is a schematic structural diagram of a fluid path monitoring device provided in an embodiment of the present disclosure;
fig. 4 is a schematic structural diagram of another fluid path monitoring device provided in the embodiment of the present disclosure;
fig. 5 shows a schematic structural diagram of a reagent selection valve provided in an embodiment of the present disclosure.
Detailed Description
In the embodiments and descriptions, if the personal information processing is involved, the processing is performed on the premise of having a validity basis (for example, obtaining the agreement of the personal information subject or being necessary for fulfilling the contract), and the processing is performed only within a specified or agreed range. The user refuses to process personal information except the necessary information required by the basic function, and the use of the basic function by the user is not influenced.
In order to make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are only a part of the embodiments of the present disclosure, not all of the embodiments. The components of the embodiments of the present disclosure, generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the disclosure, provided in the accompanying drawings, is not intended to limit the scope of the disclosure, as claimed, but is merely representative of selected embodiments of the disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the disclosure without making creative efforts, shall fall within the protection scope of the disclosure.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
The term "and/or" herein merely describes an associative relationship, meaning that three relationships may exist, e.g., a and/or B, may mean: a exists alone, A and B exist simultaneously, and B exists alone. In addition, the term "at least one" herein means any one of a variety or any combination of at least two of a variety, for example, including at least one of A, B, C, and may mean including any one or more elements selected from the group consisting of A, B and C.
Research shows that gene sequencing can analyze and determine the complete gene sequence, is favorable to predicting and preventing various diseases and is widely applied. In the gene sequencing process, various reagents are required to be added to the reaction chip to complete the gene sequencing. In this process, if a fluid path system corresponding to gene sequencing fails, the gene sequencing result may be affected. Therefore, it is very important to detect the liquid path system in real time during the gene sequencing process.
In the related art, after the gene sequencing task is completed, the fluid path system corresponding to the gene sequencing can be detected to determine whether the fluid path system has a fault.
Based on this, the fluid path system in the related art may be as shown in fig. 1. As shown in FIG. 1, the liquid path system may include a reagent selection module 11, a reaction chip 12, a pump unit 14, a detection module 13, a waste liquid tank 16, and a controller.
As can be seen from fig. 1, the liquid path system can arrange the detection module 13 in the liquid path pipeline connecting the pump unit 14 and the reagent selection module 11, and further, after the reagent selection module 11 and the pump unit 14 receive a reagent addition instruction sent by the controller, the target reagent can be transmitted to the reaction chip 12 through the liquid path pipeline. Thereafter, the reaction chip 12 may process the target reagent, and transmit the processed waste liquid to the pump unit 14 through the liquid path pipe. The pump unit 14 can then, after receiving the waste liquid, lead out the waste liquid.
According to the process, the detection module is not used in the gene sequencing process, so that the real-time detection of the existing liquid path system cannot be realized, and the liquid path system cannot be timely and effectively monitored under the condition that the liquid path system is leaked or blocked.
In the related art, when the fluid path system is to be detected, the controller sends a corresponding detection instruction to the pump set and the reagent selection module to detect the fluid path system after the gene sequencing process is completed.
However, a complete gene sequencing process takes about 30 hours, and if the liquid path system is lack of or has no sample, the pump set and the reagent selection module can be operated without liquid, which may damage the equipment and increase the maintenance cost. Moreover, if the fluid path system fails during the gene sequencing process, the gene sequencing result may be affected, thereby causing waste of the sample.
When the flow resistance of the liquid path system is detected after the gene sequencing process is completed, when it is usually necessary to flow the waste liquid into the pump set, the joint connecting the liquid path pipeline and the pump set is removed (for example, as shown in fig. 2, the joint connecting the liquid path pipeline corresponding to the liquid outlet of the reaction path 1 and the pump set port 1 needs to be removed), and the joint is connected to one end of the detection module 13 (for example, as shown in fig. 2, the joint is connected to the lower joint position of the detection module 13-1), so that the waste liquid can flow through the detection module 13-1. In the above embodiment, when the flow resistance of the liquid path system is detected, the joint needs to be repeatedly disassembled, so that the joint is easily damaged, a large amount of human resources and time cost are wasted, and the use performance of the liquid path system is also influenced.
Meanwhile, in the above embodiment, the waste liquid can flow through the liquid path channel shown by the dotted line, so that when impurities exist in the liquid path system, the impurities can easily enter the liquid path channel shown by the dotted line and further enter the pump group, and the pump group is easily damaged. In this case, not only the maintenance cost of the liquid path system is increased, but also the gene sequencing result is easily affected.
Based on the research, the present disclosure provides a liquid path monitoring device, a gene sequencer and a gene sequencing device. The liquid path monitoring device provided by the embodiment of the disclosure can comprise a reagent selection module, a reaction chip, a detection module, a pump set and a controller. The detection module can be arranged in a liquid pipeline between the reaction chip and the pump set, so that the flow resistance of flowing waste liquid can be detected through the detection module, and the flow resistance detection result is sent to the controller. Then, in a case where it is determined that the flow resistance detection is abnormal based on the flow resistance detection result, a pressure detection instruction may be sent to the pump group to perform a pressure test on the liquid path piping of the liquid path monitoring apparatus.
In the above embodiment, the detection module may be disposed in the fluid path pipeline between the reaction chip and the pump set, so as to monitor the flow resistance information in the fluid path pipeline in real time, thereby implementing real-time monitoring of the fluid path monitoring device, and determining the health status of the fluid path monitoring device in real time. And after the pressure detection instruction is received, pressure air can be transmitted to the reagent selection module based on the pump to carry out pressure test on the liquid pipeline through the detection module under the condition that the existing structure of the liquid pipeline system is not changed, so that manpower and material resources can be saved, and the cost is reduced.
For the convenience of understanding the present embodiment, a detailed description will be given to a fluid path monitoring device disclosed in the embodiments of the present disclosure.
Referring to fig. 3, which is a schematic view of a liquid path monitoring device provided in an embodiment of the present disclosure, the liquid path monitoring device includes: a reagent selection module 100, a reaction chip 200, a detection module 300, a pump set 400, and a controller 500.
The reagent selection module 100 is configured to transmit a target reagent to the reaction chip 200 through a liquid pipeline after receiving a reagent addition instruction sent by the controller 500.
In the disclosed embodiments, a rack may be included in the reagent selection module, and a plurality of reagents may be included in the rack. After the reagent selection module receives a reagent addition instruction sent by the controller, a target reagent can be selected from the plurality of reagents contained in the bracket and is transmitted to the reaction chip through the night pipeline.
In the disclosed embodiment, the reagent selection module may further comprise a reagent selection valve, a manifold and a liquid extraction needle. Wherein, the reagent selection valve is connected with the liquid taking needle through a manifold. At this time, after the reagent selection module receives a reagent addition instruction sent by the controller, the target reagent can be obtained through the liquid taking needle, and the obtained target reagent is transmitted to the reagent selection valve through the manifold. Then, the reagent selection valve can transfer the target reagent to the reaction chip through the liquid pipeline.
In the disclosed embodiment, the number of the reagent selection valves included in the reagent selection module may be one or more. In this case, after the reagent selection module receives the reagent selection command sent by the controller, the reagent selection valve may be controlled to select the corresponding target reagent based on the reagent selection command, and then the target reagent may be transferred to the reaction chip through the liquid channel.
When the number of the reagent selection valves included in the reagent selection module is plural, the target reagents selected by the respective reagent selection valves may be the same or different.
The reaction chip 200 is configured to process the target reagent, and transmit the processed waste liquid to the pump unit 400 after flowing through the detection module 300 through a liquid pipeline.
In the disclosed embodiment, the reaction chip may include at least one liquid inlet, a reaction tank, and at least one liquid outlet. Wherein, a liquid inlet can correspond to one or more liquid outlets. Wherein, the number of the liquid inlets can be the same as that of the reagent selection valves.
In the embodiment of the present disclosure, the reaction tank may include at least one reaction channel, in which case, each liquid inlet may be connected to at least one reaction channel, and each reaction channel may be connected to one liquid outlet.
In the embodiment of the disclosure, after the liquid inlet of the reaction chip receives the target reagent, the target reagent may be transferred to the corresponding reaction channel in the reaction tank for treatment, so as to obtain the treated waste liquid. And then, the treated waste liquid can be transmitted to the liquid outlets corresponding to the reaction passages, and the treated waste liquid is transmitted to the liquid passage pipeline through the corresponding liquid outlets. At this time, the other end of the waste liquid pipe is connected to a pump group.
In the embodiment of the present disclosure, a detection module may be disposed in a liquid path pipeline connecting the liquid outlet and the pump set, and flow resistance detection may be performed on the waste liquid flowing through the detection module based on the detection module. Wherein the detection module may be used to indicate a detection device, which may be a pressure sensor, for example.
The detection module 300 is configured to perform flow resistance detection on the flowing waste liquid, and send a flow resistance detection result to the controller 500.
In the embodiment of the disclosure, after the flow resistance detection is performed on the waste liquid flowing through the detection module based on the detection module, the detected flow resistance detection result may be sent to the controller.
The controller 500 is configured to send a pressure test command to the pump set 400 and a liquid feed stop command to the reagent selection module 100 and the pump set 400 in case of determining that the flow resistance detection is abnormal based on the flow resistance detection result.
In the embodiment of the disclosure, after the controller receives the flow resistance detection result, the flow resistance detection result may be compared with a preset detection result, and in a case that it is determined based on the comparison result that the flow resistance detection result is not identical to the preset detection result, it indicates that the flow resistance detection of the fluid path monitoring device is abnormal.
At this point, the controller may send a stop feed command to the reagent selection module and to the pump set to cause the pump set and reagent selection module to stop delivering the target reagent. Meanwhile, a pressure test instruction can be sent to the pump unit, so that the pump unit transmits pressure air to the liquid path pipeline in the liquid path monitoring device, and pressure test can be performed on the liquid path pipeline in the liquid path monitoring device.
The pump unit 400 is configured to control the reagent selection module 100 to obtain a target reagent after receiving a reagent addition instruction sent by the controller 500; leading out the waste liquid; and after receiving a pressure test command sent by the controller 500, the pressure test command transmits pressure air to the reagent selection module 100, so as to perform pressure test on the liquid pipeline through the detection module 300.
In an embodiment of the disclosure, the pump stack may include a pump stack selection valve and a pressure control module, and the pump stack selection valve may be in communication with the pressure control module. Where the pump set selection valve may contain a plurality of ports, the pressure control module may be indicative of a pressure control device, for example, the pressure control module may be a syringe.
In the embodiment of the present disclosure, a first port of the plurality of ports included in the pump set selection valve may be connected to a liquid path pipe, and at this time, the other end of the liquid path pipe is connected to a liquid outlet of the reaction chip. After the pump set receives the reagent addition instruction, the reagent selection module can be controlled to obtain the target reagent based on a pressure control module in the pump set and receive waste liquid based on the first port. The waste liquid may then be directed out through a third port of the plurality of ports comprised by the pump set selection valve.
A second port of the plurality of ports included in the pump group selection valve may be connected to the reagent selection valve, so that after receiving a pressure test command sent by the controller, pressure air may be transmitted to the reagent selection module based on the second port, so as to perform pressure detection on the fluid line pipeline through the detection module.
The liquid path monitoring device provided by the embodiment of the disclosure can comprise a reagent selection module, a reaction chip, a detection module, a pump set and a controller. The detection module can be arranged in a liquid pipeline between the reaction chip and the pump set, so that the flow resistance of flowing waste liquid can be detected through the detection module, and the flow resistance detection result is sent to the controller. Then, in a case where it is determined that the flow resistance detection is abnormal based on the flow resistance detection result, a pressure detection instruction may be sent to the pump group to perform a pressure test on the liquid path piping of the liquid path monitoring apparatus.
In the above embodiment, the detection module may be disposed in the fluid path pipeline between the reaction chip and the pump set, so as to monitor the flow resistance information in the fluid path pipeline in real time, thereby implementing real-time monitoring of the fluid path monitoring device, and determining the health status of the fluid path monitoring device in real time. And after the pressure detection instruction is received, pressure air can be transmitted to the reagent selection module based on the pump to carry out pressure test on the liquid pipeline through the detection module under the condition that the existing structure of the liquid pipeline system is not changed, so that manpower and material resources can be saved, and the cost is reduced.
In an alternative embodiment, the reagent selection module 100 may comprise at least one reagent selection valve, which may comprise a plurality of reagent selection ports for receiving different reagents, and a common port.
For example, the number of reagent selection valves may be 2, such as 101-1 and 101-2 shown in FIG. 4. Wherein the reagent selection port may be a PA as shown in FIG. 4 and the common port may be a VPA as shown in FIG. 4.
Here, the number of the reagent selection ports included in each reagent selection valve may be 5, 10, or 25, which is not limited in the present disclosure.
Illustratively, the reagent selection valve may be as shown in FIG. 5. The reagent selection valve in fig. 5 comprises 10 reagent selection ports, wherein the 10 reagent selection ports are "C1", "C2", "C3", …, "C9" and "C10" in fig. 5, respectively, and one common port, which may be "COM" in fig. 5.
Wherein the reagent selection port is for delivering the target reagent to the common port after receiving the target reagent. For example, as shown in fig. 5, assuming that the reagent selection port "C6" is used to receive a target reagent, at this time, the reagent selection port "C6" (i.e., may be understood as PA or PB in fig. 4) may communicate with the common port "COM" (i.e., may be understood as VPA or VPB in fig. 4) and transfer the received target reagent into the common port "COM" (i.e., may be understood as VPA or VPB in fig. 4).
In the disclosed embodiment, the common port is used for transmitting the received target reagent to the reaction chip through the liquid channel pipeline. At this time, the common port may be connected to the liquid inlet of the reaction chip through a liquid channel.
In the above embodiment, the reagent selection valve can receive different reagents and transmit the received reagents to the reaction chip, so that the requirements for processing different reagents can be met, and the structure of the reagent selection module is simplified.
In an alternative embodiment, the reaction chip 200 comprises at least one liquid inlet and at least one liquid outlet corresponding to each liquid inlet, and at least one reaction channel.
Wherein, the liquid inlets and the liquid outlets corresponding to the liquid inlets are connected through the reaction passages, and the number of the liquid inlets is the same as that of the reagent selection valves; the public port of the reagent selection valve is connected with the liquid inlet, and the liquid inlets connected with different reagent selection valves are different.
In the embodiment of the present disclosure, one liquid inlet may correspond to one reaction channel or a plurality of reaction channels. In the case where one liquid inlet corresponds to a plurality of reaction channels, the same target reagent may be received in the plurality of reaction channels.
In the embodiment of the present disclosure, each reaction passage may be connected to one liquid outlet, so that the treated waste liquid obtained in each reaction passage may be transmitted to the liquid passage pipeline through the corresponding liquid outlet.
For example, as shown in FIG. 4, the reaction chip 200 may comprise 2 liquid inlets (such as 201-1 and 201-2 in FIG. 4), 4 liquid outlets (such as 202-1, 202-2, 202-3 and 202-4 in FIG. 4), and 4 reaction channels (such as 203-1, 203-2, 203-3 and 203-4 in FIG. 4). Wherein the liquid inlet 202-1 is connected with the reaction passage 203-1 and the reaction passage 203-3, the reaction passage 203-1 is connected with the liquid outlet 202-1, the reaction passage 203-3 is connected with the liquid outlet 202-3, the liquid inlet 202-2 is connected with the reaction passage 203-2 and the reaction passage 203-4, the reaction passage 203-2 is connected with the liquid outlet 202-2, and the reaction passage 203-4 is connected with the liquid outlet 202-4.
At this time, the treated waste liquid obtained in each reaction passage may be transferred to the liquid passage pipe through the corresponding liquid outlet.
In the above embodiment, the target reagent may be received through the liquid inlet of the reaction chip and transferred to at least one reaction channel, so that the efficiency of transferring the target reagent to each reaction channel may be improved. Afterwards, the waste liquid treated in each reaction passage is transmitted to the liquid path pipeline through the liquid outlets corresponding to the reaction passages, so that the efficiency of leading out the waste liquid can be accelerated, and the efficiency of the liquid path monitoring device is improved.
In an optional embodiment, the liquid outlets of the reaction chip 200 are connected to the pump set 400, and when there are a plurality of liquid outlets corresponding to any liquid inlet, the detection module is disposed in a liquid path pipeline connecting a target liquid outlet of the plurality of liquid outlets corresponding to the liquid inlet and the pump set.
In an embodiment of the present disclosure, when there are a plurality of liquid outlets corresponding to the liquid inlet, a target liquid outlet of the plurality of liquid outlets may be at least a part of the liquid outlets of the plurality of liquid outlets.
Exemplarily, under the condition that a plurality of liquid outlets are corresponding to any one of the liquid inlets, any one of the plurality of liquid outlets corresponding to the liquid inlet can be determined as the target liquid outlet, and the detection module is arranged on a liquid path pipeline connecting the target liquid outlet and the pump set.
Or, all liquid outlets corresponding to the liquid inlet may be determined as the target liquid outlets, and a detection module may be disposed in a liquid path pipeline connecting the target liquid outlet and the pump set.
Wherein, the detection module can be a pressure sensor.
Among the above-mentioned embodiment, the liquid way pipeline that target liquid outlet and pump package that can correspond in a plurality of liquid outlets that the inlet corresponds set up detection module to can all carry out the flow resistance to the liquid way pipeline that every inlet corresponds and detect, thereby can make liquid way monitoring device's flow resistance detect more comprehensively, thereby can make the detection to liquid way monitoring device more accurate.
In an alternative embodiment, the pump stack 400 includes a pump stack selection valve 401, and the pump stack selection valve 401 includes a plurality of ports. The number of the plurality of ports included in the pump group selection valve 401 may be 6, 10, and the like, which is not limited in this disclosure so as to be implemented. The number of the pump group selection valves 401 may be one or more.
In the embodiment of the present disclosure, the liquid outlet of the reaction chip is connected to the first port of the pump set selection valve, and the first port is used for conveying the waste liquid; the second port of the pump set selection valve is connected with the reagent selection port of the reagent selection valve, and the second port is used for conveying pressure air during pressure testing.
In the disclosed embodiment, the first port indicates at least a part of the plurality of ports that the pump group selection valve includes, for example, as shown in fig. 4, in the case that the number of the pump group selection valves is 2, respectively 401-1 and 401-2, and each pump group selection valve includes 6 ports (respectively port 1, port 2, port 3, port 4, port 5, port 6), the first port of the pump group selection valve may be port 1 as well as port 2, wherein port 1 of the pump group selection valve 401-1 is used to convey waste liquid of the reaction channel 203-1, port 2 of the pump group selection valve 401-1 is used to convey waste liquid of the reaction channel 203-2, port 1 of the pump group selection valve 401-2 is used to convey waste liquid of the reaction channel 203-3, and port 2 of the pump group selection valve 401-2 is used to convey waste liquid of the reaction channel 203-4.
In the disclosed embodiment the second port indicates a different port of the plurality of ports that the pump set selection valve comprises than the first port, e.g. in case the first port is port 1 and port 2 of the pump set selection valve, as shown in figure 4, the second port may be port 6 of the pump set selection valve.
In an embodiment of the present disclosure, the pump set is further configured to, after receiving a target instruction sent by the controller, control a target port corresponding to the target instruction in a plurality of ports of the pump set selection valve to communicate, where the target instruction includes the reagent addition instruction and the pressure test instruction.
In the case where the target instruction is a reagent addition instruction, the target port corresponding to the target instruction may be understood as a first port, and at this time, the first port may be controlled to communicate with each other, so that the pump unit may receive the waste liquid.
When the target command is a pressure test command, the target port corresponding to the target command can be understood as a second port, and at this time, the communication of the second port can be controlled, so that the pump group can convey pressure air to the liquid path pipeline during the pressure test.
In the above embodiment, the pump set selection valve may include a plurality of ports, and the target instruction received by the pump set controls whether different ports in the pump set selection valve are communicated, so that different types of detection of the liquid path monitoring device may be implemented through the plurality of ports, thereby improving the test performance of the liquid path monitoring device.
In an alternative embodiment, as shown in figure 4, the pump stack 400 further comprises a pressure control module 402, the pressure control module 402 being in communication with the pump stack selection valve 401 for controlling the pressure in the fluid line conduits.
In the disclosed embodiment, the pressure control module 402 may be the injector described above, in which case the pressure in the fluid line conduit may be controlled by the injector.
In specific implementation, after receiving the reagent addition instruction, the pressure in the liquid path pipeline may be reduced (for example, air in the liquid path pipeline is sucked by an injector to reduce the pressure) to control the target reagent to enter the liquid path pipeline; after receiving the pressure test command, the pressure in the fluid line conduit may be raised (e.g., by delivering air into the fluid line conduit via a syringe to raise the pressure) so that the fluid line conduit may be pressure tested.
In the above embodiment, the pressure in the liquid pipeline can be controlled through the pressure control module, so that the automatic detection of the liquid pipeline can be realized, and the test efficiency is improved.
In an optional embodiment, the detection module 300 is further configured to: sending a pressure test result to the controller; the controller is further configured to: and controlling a display module to display pressure abnormity information under the condition that the pressure test result is detected to be abnormal.
In one possible embodiment, the detection of the abnormal pressure test result may be understood as: and the pressure holding time corresponding to the pressure test result is less than the preset holding time. The pressure holding time may be understood as a duration of time during which the pressure test result is greater than or equal to a preset pressure threshold.
In another possible embodiment, the detection of the abnormal pressure test result may be understood as: and the pressure test curve graph corresponding to the pressure test result is different from the preset pressure test curve graph.
In the embodiment of the disclosure, in the case that the pressure test result is detected to be abnormal, the display module may be controlled to display the pressure abnormality information, where the display module may be understood as a display screen of a display device connected to the controller.
In the above embodiment, the controller can receive the pressure test result, and control the display module to display the pressure abnormality information when detecting that the pressure test result is abnormal, so that the worker can be timely reminded to maintain and adjust the liquid path monitoring device, and the efficient and accurate operation of the liquid path monitoring device is ensured.
In an alternative embodiment, as shown in fig. 4, the liquid path monitoring device further includes a waste liquid barrel 600, and the waste liquid barrel 600 is connected to the pump unit 400 through a liquid path pipeline for storing the waste liquid.
Here, the waste liquid tank can be connected to a third port of a pump set selection valve in the pump set, wherein the third port is used for leading out waste liquid.
In the above embodiment, can in time derive the waste liquid through the waste liquid bucket to can guarantee liquid way monitoring device's normal operating, and in time clear away the waste liquid among the liquid way monitoring device.
It will be understood by those skilled in the art that in the above embodiments of the specific embodiments, the writing order of the steps does not imply a strict order of execution and constitutes any limitation to the implementation, and the specific order of execution of the steps should be determined by their function and possible inherent logic.
Based on the same concept, the embodiment of the disclosure also provides a gene sequencer corresponding to the liquid path monitoring device, and the liquid path monitoring device of any one of the gene sequencer. Because the principle of solving the problem of the gene sequencer in the embodiment of the present disclosure is similar to that of the liquid path monitoring device in the embodiment of the present disclosure, the implementation of the gene sequencer can refer to the implementation of the liquid path monitoring device, and repeated parts are not described again.
The embodiment of the disclosure also provides a gene sequencing device, which comprises a shell and the gene sequencer.
In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood as a specific case by those skilled in the art.
Finally, it should be noted that: the above-mentioned embodiments are only specific embodiments of the present invention, and are not intended to limit the technical solution of the present invention, and the protection scope of the present invention is not limited thereto, although the present invention is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: those skilled in the art can still modify or easily conceive of changes in the technical solutions described in the foregoing embodiments or make equivalent substitutions for some technical features within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit and scope of the embodiments of the present invention, and they should be construed as being included therein. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A fluid path monitoring device, comprising: the device comprises a reagent selection module, a reaction chip, a detection module, a pump set and a controller;
the reagent selection module is used for transmitting a target reagent to the reaction chip through a liquid pipeline after receiving a reagent adding instruction sent by the controller;
the reaction chip is used for treating the target reagent, and transmitting the treated waste liquid to the pump set after flowing through the detection module through a liquid pipeline;
the detection module is used for detecting the flow resistance of the flowing waste liquid and sending the flow resistance detection result to the controller;
the controller is used for sending a pressure test instruction to the pump set and sending a liquid inlet stopping instruction to the reagent selection module and the pump set under the condition that the flow resistance detection is determined to be abnormal based on the flow resistance detection result;
the pump group is used for controlling the reagent selection module to obtain a target reagent after receiving a reagent adding instruction sent by the controller; leading out the waste liquid; and after receiving a pressure test instruction sent by the controller, transmitting pressure air to the reagent selection module so as to perform pressure detection on the liquid pipeline through the detection module.
2. The fluid path monitoring device of claim 1, wherein the reagent selection module comprises at least one reagent selection valve; the reagent selection valve comprises a plurality of reagent selection ports for receiving different reagents and a common port;
wherein the reagent selection port is for delivering the target reagent to the common port after receiving the target reagent;
the common port is used for transmitting the received target reagent to the reaction chip through the liquid pipeline.
3. The liquid path monitoring device according to claim 2, wherein the reaction chip comprises at least one liquid inlet, at least one liquid outlet corresponding to each liquid inlet, and at least one reaction channel, the liquid inlets and the liquid outlets corresponding to the liquid inlets are connected through the reaction channel, and the number of the liquid inlets is the same as that of the reagent selection valves;
and the common port of the reagent selection valve is connected with the liquid inlet, and the liquid inlets connected with different reagent selection valves are different.
4. The liquid path monitoring device according to claim 3, wherein the liquid outlets of the reaction chip are connected to the pump set, and when there are a plurality of liquid outlets corresponding to any liquid inlet, a liquid path pipeline connecting a target liquid outlet of the plurality of liquid outlets corresponding to the liquid inlet to the pump set is provided with the detection module.
5. The fluid path monitoring device of claim 3, wherein the pump stack includes a pump stack selector valve including a plurality of ports;
the liquid outlet of the reaction chip is respectively connected with the first port of the pump set selection valve, and the first port is used for conveying the waste liquid;
the second port of the pump group selection valve is connected with the reagent selection port of the reagent selection valve, and the second port is used for conveying pressure air during pressure test;
and the pump set is also used for controlling the communication of a target port corresponding to the target instruction in a plurality of ports of the pump set selection valve after receiving the target instruction sent by the controller, wherein the target instruction comprises the reagent adding instruction and the pressure testing instruction.
6. The fluid path monitoring device according to any one of claims 1-5, wherein the pump set further comprises a pressure control module in communication with the pump set selection valve for controlling the pressure in the fluid path conduit.
7. The fluid path monitoring device of claim 1, wherein the detection module is further configured to:
sending a pressure test result to the controller;
the controller is further configured to: and controlling a display module to display pressure abnormity information under the condition that the pressure test result is detected to be abnormal.
8. The fluid path monitoring device according to claim 1, further comprising a waste fluid tank connected to the pump set through a fluid path pipe for storing the waste fluid.
9. A gene sequencer comprising the fluid path monitoring apparatus according to any one of claims 1 to 8.
10. A gene sequencing device, comprising:
a housing;
the gene sequencer of claim 9.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116144480A (en) * | 2023-04-20 | 2023-05-23 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116144480A (en) * | 2023-04-20 | 2023-05-23 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
| CN116144480B (en) * | 2023-04-20 | 2023-07-28 | 上海芯像生物科技有限公司 | Fluid system, temperature control method thereof and fluid state monitoring method |
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