CN218811697U - High-flux biological reaction device - Google Patents
High-flux biological reaction device Download PDFInfo
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- CN218811697U CN218811697U CN202223036923.1U CN202223036923U CN218811697U CN 218811697 U CN218811697 U CN 218811697U CN 202223036923 U CN202223036923 U CN 202223036923U CN 218811697 U CN218811697 U CN 218811697U
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Abstract
The utility model relates to a strain breeding device discloses a high flux biological reaction device. The device comprises a microporous plate (1), wherein a plurality of micropores (2) are formed in the microporous plate (1), at least one baffle (3) is arranged on the inner side wall of each micropore (2), each micropore (2) comprises a micropore main body (21) and a micropore closing body (22), one end of each micropore main body (21) is open, the other end of each micropore main body is connected with the micropore closing body (22), each micropore main body (21) is of a polygonal prism structure, and each micropore closing body (22) is of a conical structure. The device can provide sufficient oxygen in aerobic microorganism's cultivation screening process, increases the mixing performance and the oxygen transfer performance of cultivateing the in-process, has improved the device's suitability.
Description
Technical Field
The utility model relates to a bacterial strain breeding device specifically relates to a high flux biological reaction device.
Background
The high-throughput screening technology is a method for processing tens of millions of samples by using a deep-well plate as a culture or reaction medium and an automatic device instead of manpower at a DNA level or a cell level and sensitively and quickly acquiring data, and has the characteristics of trace amount, rapidness, sensitivity and the like.
The micropore plate is widely applied to high-throughput screening of microorganism culture in recent years, and the micropore plate is formed by arranging a plurality of micropores capable of accommodating liquid in a matrix shape, and commonly comprises a 6 micropore plate, a 12 micropore plate, a 24 micropore plate, a 48 micropore plate, a 96 micropore plate, a 192 micropore plate, a 384 micropore plate and the like, and the micropore plate has the characteristic of easy operation and can simultaneously carry out various cultures. However, when a microplate is used to establish a high-throughput screening system, the liquid volume in the reactor is usually very small, and in most established high-throughput screening systems, parameters such as oxygen transfer efficiency and mixing level cannot be controlled, and the fermentation process lacks real-time online monitoring, so that the high-throughput screening systems are difficult to understand and apply.
In screening aerobic fermentation microorganisms, it is necessary to provide sufficient dissolved oxygen for the screened microorganisms, otherwise, the screened microorganisms fail and, even if the screening is successful, may be ineffective in expanding the culture in a fermenter with sufficient oxygen supply to obtain more product. Although the current market circulating microporous plate can achieve the purpose of high flux to improve efficiency, the microporous plate can not meet the requirement of growth metabolism of aerobic microorganisms due to the particularity of the growth metabolism of the aerobic microorganisms, and the problems of low oxygen transfer performance and mixing performance, low microorganism screening flux, low screening efficiency and the like exist.
Therefore, it is highly desirable to provide a high throughput biological reaction apparatus.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that a high flux biological reaction device is provided, can provide sufficient oxygen in aerobic microorganism's cultivation screening process, increase the mixing performance and pass the oxygen performance of cultivateing the in-process, improve the device's suitability.
In order to solve the technical problem, the utility model provides a high flux biological reaction device, the device include the micropore board, be provided with a plurality of micropores on the micropore board, be provided with at least one baffle on the micropore inside wall, the micropore includes micropore main part and micropore obturator, the one end of micropore main part be opening form, the other end with the micropore obturator is connected, the micropore main part is polygon prism structure, the micropore obturator is the toper structure.
Preferably, the microporous body has a regular hexagonal prism structure.
Preferably, the cross-sectional shape of the microporous closure is circular.
Preferably, each baffle is arranged to be located on and perpendicular to a certain side of the microporous body.
Preferably, the baffle extends along the length direction of the micro-holes, and the baffle is located in the middle of the width direction of the side corresponding to the baffle.
Preferably, one end of the baffle is located at a top region of the microporous body and the other end is located at a bottom region of the microporous body.
Preferably, one end of the baffle is located at the top region of the microporous body and the other end extends to the inner sidewall of the microporous enclosure.
Preferably, a baffle is disposed on each side of the microporous body.
Preferably, the number of wells of the microplate is 6 wells, 12 wells, 24 wells, 48 wells, 96 wells or 384 wells.
Preferably, a plurality of the microwells are arranged in a matrix on the microplate.
Through the technical scheme, the beneficial effects of the utility model are that:
(1) The utility model provides a high flux biological reaction device, the micropore main part is polygon prism structure, the micropore closing body is the toper structure, set up at least one baffle on the millipore inside wall, when being used for the device aerobic microorganism's high flux to cultivate the screening, provide sufficient oxygen for the microorganism, can make microorganism and culture medium intensive mixing, increase mixing performance and the biography oxygen performance of cultivateing the in-process, improve the growth condition of microorganism, and then improve the biomass and the output rate of microorganism, the suitability of the device has also been improved.
(2) The utility model provides a high flux biological reaction device, the micropore main body is a regular hexagon prism structure, the cross section shape of the micropore closing body is round, and the oxygen transmission performance and the mixing performance of the device are further improved; every side at the micropore main part all sets up a baffle perpendicular with this side, and the one end of baffle is located the top region of micropore main part, and the other end extends to the inside wall of micropore obturator, can increase the oxygen transfer efficiency of the device, further improves the growth condition of microorganism, improves the screening ability of microorganism when guaranteeing high flux. In addition, the device also has the characteristics of simple and convenient operation, time saving and labor saving.
Other technical features and advantages of the present invention will be further described in the following detailed description.
Drawings
The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:
FIG. 1 is a schematic structural view of one embodiment of a high throughput bioreactor of the present invention;
FIG. 2 is a schematic diagram of a configuration of one embodiment of a micropore in the present invention;
FIG. 3 is a cross-sectional view of a schematic of the structure of the microwell of FIG. 2;
FIG. 4 is a top view of the high throughput biological reaction device of FIG. 1;
FIG. 5 is a 3D printing effect diagram of the high throughput biological reaction apparatus shown in FIG. 1;
FIG. 6 is a schematic diagram of the high throughput biological reaction apparatus shown in FIG. 1;
FIG. 7 is an enlarged view of a cell microscope obtained by fermentation of Schizochytrium limacinum in the high-throughput bioreactor of FIG. 1;
FIG. 8 is an enlarged view of a cell microscope obtained by fermentation of Schizochytrium limacinum in a conventional reactor.
Description of the reference numerals
1 microwell plate 2 microwell
21 microporous body 22 microporous closure
3 baffle plate
Detailed Description
The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings. It is to be understood that the description of the embodiments herein is for purposes of illustration and explanation only and is not intended to limit the invention.
It should be noted that, in the following description, some directional terms, such as "inner", "outer", "upper", "lower", "top", "bottom", etc., are used to clearly illustrate the technical solution of the present invention, and the directional terms or positional relationships indicated by the directional terms are those shown in the drawings, and are only used for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific direction, and thus, should not be interpreted as limiting the present invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected," "mounted," and "fixed" are to be construed broadly, and for example, the connection may be a fixed connection, a detachable connection, or an integral connection; the connection may be direct or indirect through an intermediate medium, and may be a communication between two components or an interaction relationship between two components. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
The utility model provides a high flux biological reaction device, the device include micropore board 1, are provided with a plurality of micropores 2 on the micropore board 1, are provided with at least one baffle 3 on micropore 2's the inside wall, and micropore 2 includes micropore main part 21 and micropore closure 22, and the one end of micropore main part 21 is the opening form, the other end is connected with micropore closure 22, and micropore main part 21 is polygon prism structure, and micropore closure 22 is the toper structure.
Through the utility model discloses above-mentioned basic technical scheme's high flux biological reaction device, during the use, see fig. 1 and fig. 2, the culture solution that will contain aerobic microorganism inoculates micropore 2, carries out fermentation culture to the microorganism, and the device can provide sufficient oxygen at the in-process that microorganism fermentation was cultivateed for the microorganism can with the culture medium intensive mixing, improves the growth condition of microorganism, and then improves the biomass and the output rate of microorganism, has also improved the device's suitability.
The utility model discloses in, micropore main part 21 is polygon prism structure, does not do the injecing to polygon prism structure, and micropore main part 21 can be the oblique prism structure, can be straight prism structure, also can be regular prism structure, for example, micropore main part 21 can be regular quadrangle prism structure, regular pentagon prism structure, regular hexagon prism structure, regular heptagon prism structure, regular octagon prism structure or regular decagon prism structure, and technical personnel can design according to particular case in this field. As a preferred embodiment of the microporous body 21 of the present invention, referring to fig. 3, the microporous body 21 has a regular hexagonal prism structure to increase the oxygen transmission capacity of the device.
The utility model discloses in, micropore obturator 22 is the toper structure, does not limit to the toper structure, for example, micropore obturator 22 can be pyramid structure, also can be the cone structure, and the technical personnel in this field can design according to particular case. As a preferred embodiment of the microporous closure 22 of the present invention, the cross-sectional shape of the microporous closure 22 is circular to facilitate manufacturing.
The utility model discloses do not do the injecion baffle 3 and micropore main part 21's connected mode, baffle 3 can be connected with a certain side of micropore main part 21 with arbitrary angle, for example, baffle 3 can set up to be connected perpendicularly with a certain side of micropore main part 21, also can set up to be connected with a certain side slope of micropore main part 21, and technical personnel can design according to particular case in this field. As the utility model discloses a preferred embodiment that well baffle 3 and micropore main part 21 are connected, every baffle 3 sets up to be located micropore main part 21 a certain side on and perpendicular with this side, this kind of mode of setting, processing is simple and convenient, excellent in use effect.
The utility model discloses do not do the injecion to baffle 3, baffle 3 can extend along 2 length direction of micropore, also can extend along 2 circumference of micropore, and baffle 3 can be located the side width direction's that this baffle 3 corresponds middle part, also can be located the side width direction's that this baffle 3 corresponds both ends, and technical personnel can design according to particular case in the art. As the utility model discloses a preferred embodiment of well baffle 3, baffle 3 extends along the length direction of micropore 2, and baffle 3 is located the side width direction's that this baffle 3 corresponds middle part, is favorable to the mixing of microorganism and culture solution in the micropore 2, improves the device's biography oxygen performance and mixing performance.
As a specific embodiment of the middle baffle 3 of the present invention, the top region of the micro-porous body 21 and the bottom region of the micro-porous body 21 are located at one end of the baffle 3, and the oxygen transfer performance and the mixing performance of the device can be further improved.
As another embodiment of the baffle 3 of the present invention, one end of the baffle 3 is located at the top region of the microporous main body 21, and the other end extends to the inner side wall of the microporous closing body 22, so that the oxygen transmission performance and mixing performance of the device can be further improved.
In the utility model, the specific shape of the baffle 3 is not limited, for example, the baffle 3 can be a cuboid or a cube, and the technical personnel in the field can design according to the specific situation; the number of the baffle 3 is not limited, for example, one or more baffles 3 may be provided, and the skilled person may design the baffle according to the specific situation.
The utility model discloses in, when the quantity of baffle 3 is a plurality of, a plurality of baffles 3 can all be located a certain side of micropore main part 21, and a plurality of baffles 3 can disperse on micropore main part 21's different sides. As the utility model discloses well baffle 3 and another kind of preferred embodiment that micropore main part 21 is connected, all be provided with baffle 3 on every side of micropore main part 21, can improve the fermentation effect of microorganism, obtain better fungus ball form.
The utility model discloses do not do the restriction to the hole number of micropore board 1, the hole number of micropore board 1 is 6 holes, 12 holes, 24 holes, 48 holes, 96 holes or 384 holes, and technical personnel can design according to particular case in the field. As a specific embodiment of the utility model discloses well micropore board 1, the hole number of micropore board 1 is 96 holes, can provide better growing environment for the microorganism to improve the screening flux and the screening efficiency of microorganism.
The utility model discloses do not limit to the size of micropore board 1, technical personnel can adjust the size of a dimension of micropore board 1 according to micropore 1's hole number in this field.
The utility model discloses do not injecing the distribution mode of micropore 2 on micropore board 1, micropore 2 can be with the form evenly distributed of array on micropore board 1, and micropore 2 also can adopt the mode of inhomogeneous distribution to distribute on micropore board 1. As the utility model discloses well micropore 2 is a preferred embodiment that distributes on micropore board 1, and a plurality of micropores 2 are the matrix arrangement on micropore board 1, can make things convenient for the production to make things convenient for technical staff's operation to use also.
The utility model discloses do not do the injecion to micropore 2's volume, can satisfy aerobic microbe growth metabolism's demand and the detection demand of microorganism can, for example, micropore 2's volume can be 1-3mL, and technical personnel in the field can design according to particular case.
The utility model discloses in, high flux biological reaction device can also include sealing device, for example, aseptic membrane seals, and the device can provide the dissolved oxygen of different degree for the microenvironment in the micropore 2 through the aperture size of aseptic membrane, overcomes culture solution pollution, the oxygen suppliment is not enough and the too fast scheduling problem of moisture evaporation.
As a relatively preferred embodiment of the middle and high flux bioreactor of the present invention, refer to FIGS. 1 to 6The device includes micropore board 1, be provided with 96 micropores 2 on micropore board 1, be provided with 6 baffles 3 on micropore 2's the inside wall, micropore 2 includes micropore main part 21 and micropore closure 22, micropore main part 21's one end is the opening form, the other end is connected with micropore closure 22, micropore main part 21 is regular hexagon prism structure, micropore closure 22 is the toper structure, micropore closure 22's cross sectional shape is circular, all be provided with baffle 3 on every side of micropore main part 21, every baffle 3 sets up to be located a certain side of micropore main part 21 and perpendicular with this side, baffle 3 extends along micropore 2's length direction, and baffle 3 is located the middle part of the side width direction that this baffle 3 corresponds, the one end of baffle 3 is located the top region of micropore main part 21, the other end extends to micropore closure 22's inside wall, 96 micropores 2 are the matrix arrangement on micropore board 1, micropore 2's height is 25mm, micropore main part 21's cross sectional area is 56mm 2 The size of the baffle 3 is 0.8mm × 1mm × 22mm.
The specific use process of the high-throughput biological reaction device provided by the above embodiment is as follows:
(1) Adding a culture medium containing schizochytrium limacinum into the micropores 2 of the high-flux biological reaction device, wherein the culture medium is glucose 40g/L, KH 2 PO 4 3g/L、Na 2 SO 4 12g/L、MgSO 4 4g/L、KCl 2g/L、(NH 4 ) 2 SO 4 4g/L、CaCl 2 0.08g/L、K 2 SO 4 0.6g/L, 10g/L of monosodium glutamate and 12g/L of yeast extract powder, wherein the inoculation amount of the culture medium in each micropore 2 is 20 percent of the volume of each micropore 2;
(2) Fixing the high-flux biological reaction device on a 96-plate shaking table, and carrying out fermentation culture under the conditions of: the temperature is 28 ℃, the time is 48h, and the stirring speed is 800rpm;
the morphology of the myclobules of Schizochytrium was determined and the results are shown in FIG. 7.
The utility model discloses in, still add the above-mentioned culture medium that contains schizochytrium into conventional reactor, this conventional reactor compares with the high flux biological reaction device that above-mentioned embodiment provided, and the difference is, micropore 2 is the cylinder structure, and 2 one ends of micropore are the opening form, the other end is the closure form, and 2 insides of micropore do not set up baffle 3, and the volume of micropore 2 is 1.5mL, cultivates with the same condition to survey the fungus ball form, the result is shown in figure 8.
As can be seen from the above description, the utility model provides a high flux biological reaction device through set up baffle 3 on every side of micropore main part 2, has improved the device's biography oxygen performance and mixing performance, has effectively improved aerobic microorganism's growth condition and screening efficiency to obtain better fungus ball form.
The preferred embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited thereto. In the technical concept scope of the present invention, it can be right to perform various simple modifications to the technical solution of the present invention, including combining each specific technical feature in any suitable manner, in order to avoid unnecessary repetition, the present invention does not describe separately various possible combinations. These simple variations and combinations should also be considered as disclosed in the present invention, all falling within the scope of protection of the present invention.
Claims (10)
1. The high-flux biological reaction device is characterized by comprising a microporous plate (1), wherein a plurality of micropores (2) are formed in the microporous plate (1), at least one baffle (3) is arranged on the inner side wall of each micropore (2), each micropore (2) comprises a micropore main body (21) and a micropore closing body (22), one end of each micropore main body (21) is in an opening shape, the other end of each micropore main body is connected with the micropore closing body (22), each micropore main body (21) is of a polygonal prism structure, and each micropore closing body (22) is of a conical structure.
2. The device according to claim 1, wherein the microporous body (21) is a regular hexagonal prism structure.
3. The device according to claim 2, wherein the cross-sectional shape of the microporous closure (22) is circular.
4. The device according to claim 1, characterized in that each baffle (3) is arranged on a side of the microporous body (21) perpendicular to it.
5. The device according to claim 4, wherein the baffle (3) extends along the length direction of the micropores (2), and the baffle (3) is located at the middle of the width direction of the corresponding side of the baffle (3).
6. The device according to claim 5, characterized in that the baffle (3) has one end located in the top region of the microporous body (21) and the other end located in the bottom region of the microporous body (21).
7. The device according to claim 5, characterized in that the baffle (3) has one end located in the top region of the microporous body (21) and the other end extending to the inner side wall of the microporous enclosure (22).
8. The device according to any one of claims 1 to 7, characterized in that a baffle (3) is provided on each side of the microporous body (21).
9. The device according to any of claims 1 to 7, wherein the number of wells of the microplate (1) is 6, 12, 24, 48, 96 or 384 wells.
10. The device according to any one of claims 1 to 7, wherein a plurality of the microwells (2) are arranged in a matrix on the microplate (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223036923.1U CN218811697U (en) | 2022-11-15 | 2022-11-15 | High-flux biological reaction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223036923.1U CN218811697U (en) | 2022-11-15 | 2022-11-15 | High-flux biological reaction device |
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| Publication Number | Publication Date |
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| CN218811697U true CN218811697U (en) | 2023-04-07 |
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| CN202223036923.1U Active CN218811697U (en) | 2022-11-15 | 2022-11-15 | High-flux biological reaction device |
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