CN218811643U

CN218811643U - Be used for manual magnetic rod cover isolation frame that draws of 96 orifice plates nucleic acid

Info

Publication number: CN218811643U
Application number: CN202222534681.2U
Authority: CN
Inventors: 祝艺懿; 先志强; 谭婷婷
Original assignee: Baoguang Biotechnology Research Chongqing Co ltd
Current assignee: Baoguang Biotechnology Research Chongqing Co ltd
Priority date: 2022-09-26
Filing date: 2022-09-26
Publication date: 2023-04-07
Anticipated expiration: 2032-09-26

Abstract

The utility model relates to a molecular biological experiment equipment field discloses a be used for manual extraction bar magnet cover spacer for 96 orifice plate nucleic acid, and the spacer is cavity cuboid structure, can paste and place on 96 deep hole board for there is certain space between the bottom of bar magnet cover and the 96 orifice plate bottom, avoids the magnetic bead to gather under the action of gravity and can't lead to nucleic acid extraction failure or quality to reduce in the dispersion of follow-up cleaning process. The isolation frame can be taken up together with the 8-linked magnetic rod sleeve, the magnetic rod sleeve is prevented from being confused to pollute a sample, the operation is convenient, the structure is simple, the manufacturing cost is low, and the market prospect is wide.

Description

Be used for manual magnetic rod cover isolation frame that draws of 96 orifice plates nucleic acid

Technical Field

The invention relates to the field of molecular biological experimental equipment, in particular to a magnetic rod sleeve isolation frame for manually extracting nucleic acid from a 96-pore plate.

Background

The magnetic bead method for extracting nucleic acid is widely applied at present, and a 96-well plate is a necessary experimental instrument for rapidly extracting multiple samples. The magnetic bead method is used for extracting nucleic acid by adsorbing the magnetic beads with a magnet, and for a sample in a 96-well plate, the magnetic beads are generally enriched by placing the sample in a magnetic sleeve frame with the magnet. However, the magnetic sleeve frame with the magnet is generally heavy, magnetic beads gathered at the bottom of the magnetic rod sleeve are possibly pressed to the bottom of a 96-well plate in the process of manually extracting nucleic acid or automatically extracting nucleic acid, and the magnetic beads are agglomerated under the pressure to be not beneficial to subsequent cleaning of a sample, so that the nucleic acid extraction quality is influenced; in addition, when the magnetic rod sleeves are manually extracted in an 8-link mode, the magnetic rod sleeves are easy to be mixed after being placed, the magnetic rod sleeves need to be marked on a magnetic sleeve frame, and meanwhile, the magnetic rod sleeves are not easy to move.

In order to overcome the defects, the design is innovated and researched to design a magnetic rod sleeve isolation rack for manually extracting the nucleic acid from the 96-hole plate.

Disclosure of Invention

The utility model discloses the main problem of solving provides an use isolation frame at the manual extraction magnetic rod cover of 96 orifice plate nucleic acid to solve the manual or automatic extraction in-process of nucleic acid, enrich under the action of bar magnet gravity or under the action of automatic machine mechanical force in the inseparable sediment that the magnetic bead of magnetic rod cover bottom gathered, and can't break up in follow-up cleaning process, make salt, protein or other impurity can't effectively by the sanitization, and a plurality of magnetic rod cover easily obscure the problem of polluting the sample.

For solving the technical problem, the utility model relates to a technical scheme who adopts is: a magnetic rod sleeve isolation frame for manually extracting nucleic acid from a 96-well plate comprises a frame body and a hollow part, and can be used for simultaneously placing 12 8-linked magnetic rod sleeves without confusion.

As a further optimization of the above-mentioned solution, the isolation frame body is made of polypropylene.

As a further optimization of the above scheme, the spacer is placed against the deep hole plate.

As a further optimization of the scheme, the magnetic sleeve frame is placed on the isolation frame, so that a certain distance is reserved between the magnetic sleeve frame and the deep hole plate.

As a further optimization of the scheme, 12 8-linked magnetic rod sleeves can be placed on the isolation frame at the same time.

The utility model provides an at the manual isolation frame that draws bar magnet cover of 96 orifice plate nucleic acid, this isolation frame can be fixed in on the 96 orifice plate of depth, and the bar magnet cover that has magnet can be arranged in the deep hole board through the well kenozooecium in the isolation frame, and the existence of isolation frame makes the bottom and the 96 orifice plate bottom of bar magnet cover separate the certain distance, even if be convenient for observe the inboard solution condition, can not press the magnetic bead of bar magnet cover bottom enrichment at the bottom of the tube again. Meanwhile, the magnetic rod sleeves are arranged on the isolation frame and can move simultaneously, and a single magnetic rod sleeve can be taken out, so that the operation is convenient. And the structure is simple, the manufacturing cost is low, and the market prospect is wide.

Drawings

FIG. 1 is a schematic view of a spacer structure;

FIG. 2 is a schematic side view of an isolator frame;

FIG. 3 is a schematic side view of the isolation frame;

Detailed Description

For better illustrating the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments.

Example 1

As shown in FIG. 1, an isolation rack for a magnetic rod sleeve for manually extracting nucleic acid from a 96-well plate comprises a frame body and a hollow part.

The specific using process is as follows:

in the extraction process of the 96-well plate for manually extracting nucleic acid, an isolation frame and the four corners of the 96-well plate are oppositely arranged on the 96-well plate, then a sample and a reagent are added into the 96-well plate, and a magnetic rod sleeve with a magnet is placed into the 96-well plate through the hollow part of the isolation frame for extracting nucleic acid. Due to the existence of the isolation frame, a certain gap exists between the bottom of the magnetic rod sleeve and the bottom of the plate, and magnetic beads enriched at the bottom of the magnetic rod sleeve are prevented from being aggregated under the action of gravity to influence subsequent cleaning. When the magnetic rod sleeve needs to be taken out to add the reagent into the 96-well plate, the isolation frame can be taken up together, and the isolation frame with the magnetic rod sleeve is put back together after the reagent is added, so that sample pollution caused by confusion of the magnetic rod sleeve is avoided.

Claims

1. The utility model provides a be used for manual extraction bar magnet cover spacer of 96 orifice plates nucleic acid which characterized in that: the isolation frame comprises a frame body and a hollow part, the isolation frame body is placed in a manner of being attached to the deep hole plate, and the magnetic sleeve frame is placed on the isolation frame through the hollow part, so that a certain distance is separated between the magnetic sleeve frame and the deep hole plate.

2. The spacer of claim 1, wherein the spacer frame body is a polypropylene material.

3. The spacer of claim 1 wherein 12 8-linked magnetic rod sleeves can be placed simultaneously.