CN218675482U - Epidiascope with free-profile field of view diaphragm - Google Patents
Epidiascope with free-profile field of view diaphragm Download PDFInfo
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- CN218675482U CN218675482U CN202223424271.9U CN202223424271U CN218675482U CN 218675482 U CN218675482 U CN 218675482U CN 202223424271 U CN202223424271 U CN 202223424271U CN 218675482 U CN218675482 U CN 218675482U
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Abstract
The utility model provides an epidiascope with free profile field of view diaphragm, it adopts printing opacity LCD display screen as the field of view diaphragm, realizes that the profile and the luminousness of field of view diaphragm freely adjust. The device comprises a tube lens, a semi-transparent and semi-reflective lens, an objective lens and an objective table which are positioned on the same optical axis, wherein a light source, a view field diaphragm and the semi-transparent and semi-reflective lens are arranged on the other optical axis, and the view field diaphragm is a light-transmitting LCD display screen; the light emitted by the light source is reflected by the semi-transparent semi-reflecting mirror after passing through the light-transmitting LCD display screen and forms a conjugate image of the light-transmitting LCD display screen on the objective table through the objective lens, and the conjugate image is used for illuminating a sample on the objective table, and the reflected light of the sample forms a sample image through the objective lens, the semi-transparent semi-reflecting mirror and the tube mirror.
Description
Technical Field
The utility model relates to an epi-microscope, especially fluorescence microscope with field of view diaphragm.
Background
The field diaphragm of the existing epi-microscope with the field diaphragm adopts an aperture structure which is similar to that of a camera and is mainly formed by a plurality of sheets in a surrounding way, and the size of a central hole formed by the sheets is changed through the relative rotation of the sheets. The diaphragm has the advantages of complex structure, high requirement on preparation precision and high cost, and the central hole of the diaphragm is generally only a polygon similar to a circle. Therefore, when light passes through the field stop, the contour of the conjugate image on the stage passing through the objective lens is generally circular, but cannot be other shapes. Because the traditional view field diaphragm is a circular field with adjustable size, an illumination field formed on the object plane of the epi-polar microscope is a target to be observed in a free contour form (particularly fluorescence observation), and interference stray light can be formed because the illumination system illuminates the target in a non-observation area to influence the signal-to-noise ratio of the observation.
Disclosure of Invention
The utility model aims at providing an epidiascope with free profile field of view diaphragm, it has abandoned the field of view diaphragm of traditional mechanical light ring structure, adopts printing opacity LCD display screen as the field of view diaphragm, realizes the profile and the luminousness free adjustment of field of view diaphragm, the cost is reduced.
The epidiascope with the free profile view field diaphragm is provided with a tube lens, a semi-transparent and semi-reflective lens, an objective lens and an objective table which are positioned on the same optical axis, wherein a light source, the view field diaphragm and the semi-transparent and semi-reflective lens are arranged on the other optical axis, and the view field diaphragm is a light-transmitting LCD display screen; the light emitted by the light source passes through the light-transmitting LCD display screen, then is reflected by the semi-transmitting semi-reflecting mirror and forms a conjugate image of the light-transmitting LCD display screen on the objective table through the objective lens, the conjugate image is used for illuminating a sample positioned on the objective table, and the reflected light of the sample forms a sample image through the objective lens, the semi-transmitting semi-reflecting mirror and the tube lens.
In the epi-beam microscope, the optical filter is disposed between the half mirror and the field stop.
In the epi-microscope, the conjugate image of the transparent LCD display screen is the same as the contour of the sample.
According to the epi-microscope, the whole LCD display screen is transparent, and the whole LCD display screen is the same as the outline shape of a sample.
In the epi-microscope, the LCD display screen is partially transparent, and the transparent portion, i.e., the transparent LCD display screen, has the same contour shape as the sample.
In the epi-microscope, the output of the controller is connected with the LCD display screen, and the controller applies voltage to the liquid crystal in the part, needing light transmission, of the LCD display screen to enable the part to transmit light.
The utility model has the advantages that:
an LCD (Liquid Crystal Display) Display screen belongs to the prior art, namely a Liquid Crystal Display screen. The LCD is constructed by placing a liquid crystal cell between two parallel glass substrates, arranging TFT (thin film transistor) on the lower substrate glass, arranging a color filter on the upper substrate glass, and controlling the rotation direction of liquid crystal molecules by changing signals and voltages on the TFT, thereby achieving the purpose of controlling whether polarized light of each pixel point is emitted or not to achieve the display. The liquid crystal looks like a liquid from the shape and appearance, but its crystal-like molecular structure shows the form of a solid. However, when a voltage is applied to the liquid crystal, the molecules are vertically rearranged, so that light can be directed out.
The utility model discloses use the printing opacity LCD display screen on the microscope as the visual field diaphragm, realize controllable free profile visual field diaphragm and controllable subregion freely see through the decay diaphragm. Since the liquid crystal in the area needing light transmission on the LCD screen can be applied with voltage to make the part light transmission, the light transmission part can be adjusted to any shape (contour) conveniently, even the shape consistent with the sample needing observation. By changing the control voltage of local pixels, the deflection angle of the liquid crystal can be changed to change the local transmittance of the diaphragm target, so that the illumination intensity of a local target area can be controlled, and the control precision of the diaphragm outline and the intensity can be accurate to the pixel level. Of course, the whole LCD screen can be transparent, and the whole LCD screen has the same outline shape as the sample.
Of course, an optical filter may be disposed between the half-mirror and the field stop, so as to realize illumination of different colors, especially for a fluorescence microscope, fluorescent illumination of different wavelengths may be realized.
Drawings
FIG. 1 is a schematic diagram of an epitactic microscope with a free-profile field of view diaphragm.
Detailed Description
Referring to the epi-illumination microscope with the free contour field diaphragm shown in fig. 1, the CCD1, the tube lens 2, the half mirror 3, the objective lens 4, and the stage 5 are located on the same optical axis, and the light source 8, the field diaphragm 7, the filter 6, and the half mirror 3 are arranged on another optical axis.
The viewing diaphragm 7 comprises an LCD display screen body 71, a controller 72, a power supply and the like.
The LCD display screen body partially transmits light, and the light transmitting part of the LCD display screen body is the same as the outline shape of a sample on the objective table to be observed. The output of the controller is connected with the LCD display screen body, and the controller loads voltage on liquid crystal in the part, needing light transmission, of the LCD display screen body, so that the part transmits light and the transmittance of the light transmission part can be controlled.
Light emitted by the light source passes through the light-transmitting LCD screen (namely the light-transmitting LCD screen at the light-transmitting part of the LCD screen body), is filtered by the light filter, is reflected by the semi-transparent and semi-reflective mirror and forms a conjugate image of the light-transmitting LCD screen on the objective table through the objective lens, and is used for illuminating a sample on the objective table, and reflected light of the sample passes through the objective lens, the semi-transparent and semi-reflective mirror and the tube mirror and is received by the CCD to form sample image output.
The light-transmitting LCD screen replaces the traditional diaphragm, and the voltage is adjusted through the controller to form a view field diaphragm with any shape and any local transmittance.
Claims (6)
1. The epi-beam microscope with free contour view field diaphragm is provided with a tube lens, a semi-transparent and semi-reflective lens, an objective lens and an objective table which are positioned on the same optical axis, and a light source, the view field diaphragm and the semi-transparent and semi-reflective lens are arranged on the other optical axis; the light emitted by the light source is reflected by the semi-transparent semi-reflecting mirror after passing through the light-transmitting LCD display screen and forms a conjugate image of the light-transmitting LCD display screen on the objective table through the objective lens, and the conjugate image is used for illuminating a sample on the objective table, and the reflected light of the sample forms a sample image through the objective lens, the semi-transparent semi-reflecting mirror and the tube mirror.
2. The epidiascope of claim 1 wherein an optical filter is disposed between the half-mirror and the field stop.
3. The epitactic microscope of claim 1, wherein the conjugate image of the light transmissive LCD display is the same shape as the contour of the specimen.
4. The epitactic microscope of claim 3, wherein the entire LCD panel is transparent and the entire LCD panel has the same contour as the specimen.
5. The epitactic microscope of claim 3 wherein the LCD display is partially transparent and the transparent portion of the LCD display is substantially the same shape as the contour of the specimen.
6. The epitactic microscope of claim 5, wherein the output of the controller is connected to an LCD display screen, and wherein the controller applies a voltage to liquid crystal in a portion of the LCD display screen that is required to transmit light to cause the portion to transmit light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223424271.9U CN218675482U (en) | 2022-12-20 | 2022-12-20 | Epidiascope with free-profile field of view diaphragm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223424271.9U CN218675482U (en) | 2022-12-20 | 2022-12-20 | Epidiascope with free-profile field of view diaphragm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218675482U true CN218675482U (en) | 2023-03-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223424271.9U Active CN218675482U (en) | 2022-12-20 | 2022-12-20 | Epidiascope with free-profile field of view diaphragm |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218675482U (en) |
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2022
- 2022-12-20 CN CN202223424271.9U patent/CN218675482U/en active Active
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