CN218629191U

CN218629191U - Sample application station module and liquid-transfering sample application workstation

Info

Publication number: CN218629191U
Application number: CN202222925501.3U
Authority: CN
Inventors: 周夫之; 王羿; 何萍萍; 陈浩; 施德盛; 叶明汉
Original assignee: Shanghai Ruidu Photoelectric Technology Co ltd
Current assignee: Shanghai Ruidu Photoelectric Technology Co ltd
Priority date: 2022-11-03
Filing date: 2022-11-03
Publication date: 2023-03-14
Anticipated expiration: 2032-11-03

Abstract

The application provides sample application station module and move liquid sample application workstation. The sample application station module comprises a first base (61), wherein a mounting groove (611) is formed in the first base (61), a through hole (614) penetrating through the first base (61) in the thickness direction of the first base (61) is formed in the mounting groove (611), the mounting groove (611) is rectangular, and a first redundant space (612) is formed in at least one of four corners of the mounting groove (611). The pipetting spotting station comprises: the sample application station module (6); a droplet module (3), the droplet module (3) comprising a needle (334) capable of controlled aspiration and ejection of liquid; and a cleaning module (7), the cleaning module (7) being capable of cleaning the needle (334) of the droplet module (3).

Description

Sample application station module and liquid-transfering sample application workstation

Technical Field

The application relates to a sample application station module and a liquid-transfering sample application workstation.

Background

In, for example, a genechip spotting device, a spotting station is typically included. After placing the chip in the spotting station, a spotting operation may be performed on the chip.

CN206057045U shows a biochip spotting instrument substrate fixing device, which fixes the position of the chip through the groove. However, the difficulty of taking of its chip is higher, and the reason includes the cooperation of rectangle chip and rectangle recess after, four right angles of chip interfere with four right angles of recess easily and cause the damage of chip at the in-process of taking easily. In addition, under the condition that the groove is smooth or water exists in the groove, the air pressure between the chip and the groove is small, and the chip is not easy to take out of the groove according to the vacuum adsorption principle.

SUMMERY OF THE UTILITY MODEL

The application provides sample application station module and move liquid sample application workstation.

This sample application station module includes first base, be provided with the mounting groove in the first base, be provided with in the mounting groove run through in the thickness direction of first base the through-hole of first base, the mounting groove is the rectangle, at least one corner in the four corners of mounting groove is provided with first redundant space.

In at least one embodiment, at least one of the two pairs of opposite sides of the mounting groove is provided with a second redundant space.

In at least one embodiment, the deposition station module further comprises a base post and a second base, the first base being detachably connected to the second base by the base post.

The application provides a move liquid spotting workstation includes: a spotting station module as described above;

the liquid drop module comprises a needle head capable of being controlled to suck and spray liquid; and

a cleaning module capable of cleaning the needle of the droplet module.

In at least one embodiment, the cleaning module comprises a cleaning zone comprising:

a cleaning tank in which a discharge port for discharging liquid is provided;

the cleaning device comprises a cleaning column, wherein the cleaning column is arranged in the cleaning tank, one or more cleaning holes are formed in the cleaning column, cleaning liquid can be vertically and upwards sprayed out of the cleaning holes, and the spraying height of the cleaning liquid is larger than the highest point of the cleaning column.

In at least one embodiment, the top surface of the cleaning column comprises two surfaces with different inclination degrees, a turning part is arranged between the two surfaces with different inclination degrees, and the turning part penetrates through the circle center of the cleaning hole.

In at least one embodiment, the two surfaces that differ in degree of inclination include a horizontal surface and an inclined surface.

In at least one embodiment, the height of the slope gradually decreases away from the turn.

In at least one embodiment, the ramp is further from the print station module than the horizontal plane.

In at least one embodiment, the needle comprises a flow passage wall and a flow path in the flow passage wall, the flow passage wall having a waistline which, in a cross-section along the axis of the needle, makes an angle α with the axis which satisfies: alpha is less than or equal to 30 degrees, and the cross section of the flow path is circular.

This application is through setting up the through-hole in the mounting groove to reduce the adsorption affinity between product and the mounting groove, be convenient for taking of product. The right angle on the mounting groove is provided with a redundant space, so that the possibility that the product is damaged due to interference with the right angle position in the mounting and dismounting process of the mounting groove is reduced.

The application provides a move liquid sample application workstation and have above-mentioned advantage because of having above-mentioned sample application workstation module.

Drawings

Fig. 1 shows a schematic view of the overall structure of a pipetting station according to an embodiment of the application.

Fig. 2 shows a schematic view of a part of the structure of a pipetting printing station according to an embodiment of the application.

Fig. 3 shows a schematic view of a droplet module of a pipetting station according to an embodiment of the application.

Fig. 4 shows a schematic structural view of the droplet module body of the droplet module in fig. 3.

Fig. 5 shows a schematic view of the needle of the drop module body of fig. 4.

Fig. 6 shows a schematic of the structure of the printing station module and the washing module of a pipetting printing station according to an embodiment of the application.

Fig. 7 shows a top view of a mounting slot in a print station module of a pipetting print station according to an embodiment of the application.

Fig. 8 shows an isometric view of a wash zone of a wash module of a pipetting station according to an embodiment of the application.

Fig. 9 shows a top view of the cleaning zone of fig. 8.

Fig. 10 shows a side view of the guide and the base of the dry zone of the washing module of the pipetting station according to an embodiment of the application.

Description of the reference numerals

1, a frame; 11 a substrate;

2, a motion module; 21 a first slider; 22 a second slide; 23 a third slide block;

3 a droplet module; 31, a gas source; 32 a gas source controller; 33 a droplet body module; 331 a first flow channel; 332 a second flow channel; 333 a third flow channel; 3331 a first end; 3332 a second end; 3333 the end is open; 334 needle head; 3341 a first end; 3342 a second end; 335 a housing structure; 336 a flow passage control structure; 3361 sealing the stopper; 3362 a connecting member; 3363 a main body; 3371 a first baffle; 3372 a second baffle;

4, a detection module; 41 a detection camera; 42 a supplementary lighting device;

5 a reagent module;

6 sample application station module; 61 a first base; 611 installing the groove; 612 a first redundant space; 613 a second redundant space; 614 through holes; a 62-base pillar; 63 a second base;

7, a cleaning module; 71 a cleaning area; 711 cleaning tank; 7111 a discharge port; 712 washing the column; 7121 washing holes; 7122 a turn; 7123 horizontal plane; 7124 a bevel; 7125 an arc-shaped face; 713 connecting blocks; 72 a drying zone; 721 drying the block; a base 722; 723 guide rails;

l1 waist line; the L2 axis.

Detailed Description

Exemplary embodiments of the present application are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the present application, and is not intended to be exhaustive or to limit the scope of the application.

The application embodiments provide a pipetting spotting workstation. Referring to fig. 1, 2, the pipetting and spotting workstation may comprise a frame 1, a motion module 2, a droplet module 3 (not shown in the figure), a detection module 4, a reagent module 5, a spotting station module 6, a washing module 7.

Referring to fig. 1 and 2, the frame 1 may include a substrate 11, a housing, and an electrical cabinet. The motion module 2, the reagent module 5, the spotting station module 6, and the cleaning module 7 may be mounted on the substrate 11. The bottom of the substrate 11 may be provided with an electrical cabinet. The casing can set up materials such as transparent glass, plastics, when playing certain sealed effect, conveniently observes moving liquid, the sample application process.

See fig. 2, where X, Y, Z are oriented perpendicular to each other. The motion module 2 may be mounted to the base plate 11. The motion module 2 may be a three-axis motion platform, such that the droplet module 3 (not shown) and the detection module 4 mounted on the motion module 2 can realize motion in three directions X, Y, Z, for example.

For example, the motion module 2 may be composed of a slider, a guide rail, a motor, and the like. For example, two rails are arranged side by side in the Y direction. Of course, two guide rails can be arranged in parallel in the X direction and the Z direction, so that the stability is improved. The slides may include a first slide 21 moving in the Z direction, a second slide 22 moving in the X direction, and a third slide 23 moving in the Y direction. The slide blocks may be provided with guide rails for guiding, the second slide block 22 may be or include the guide rails of the first slide block 21, and the third slide block 23 may be or include the guide rails of the second slide block 22. The droplet module 3 and the detection module 4 can be connected to the first slide block 21, and further can realize the movement in three directions X, Y, Z.

Referring to fig. 3, the droplet module 3 may include a gas source 31, a gas source controller 32, and a droplet module body 33, which are connected in sequence. The droplet module 3 can perform the functions of sucking and ejecting liquid. Wherein the gas source 31 may comprise a gas tank or the like. The air source controller 32 may be a valve, an air pressure sensor, or the like, and the droplet module body 33 may be a nozzle, and the liquid may be sucked and ejected by controlling the air pressure of the droplet module body 33, for example, by providing negative pressure and positive pressure to the droplet module body 33.

Referring to fig. 4, droplet module body 33 can include flow channels, which can include a first flow channel 331, a second flow channel 332, a third flow channel 333, a needle 334, a housing structure 335, and a flow control structure 336. The first flow channel 331, the second flow channel 332, and the third flow channel 333 may be disposed inside the housing structure 335.

The first flow path 331 may be a cylindrical flow path, which communicates with the gas source controller 32 and the second flow path 332.

The cross-section of the second flow passage 332 may be a circular ring. The second flow passage 332 communicates with the first flow passage 331 and the third flow passage 333. Of course, the cross-sections of the second flow passages 332 may not be all circular rings, and the cross-section of the position where the second flow passages 332 are connected to the third flow passages 333 may be circular rings.

The first end 3331 of the third flow path 333 communicates with the second flow path 332. The first end 3331 may be configured as a hollow cylinder and may have an annular cross-section, and the second end 3332 of the third flow passage 333 may have an annular cross-section. It can be understood that the third flow channel 333 and the second flow channel 333 with circular cross-sections expand the volume capable of containing liquid as much as possible, expand the amount of liquid capable of being ejected in a single time, and can realize picoliter and nanoliter sample application and also perform microliter and milliliter sample application.

The second end 3332 of the third flow passage 333 may be deflected radially inward with the end opening 3333 of the second end 3332 being adjacent the needle 334. Further, the end opening 3333 of the second end 3332 may face radially inward.

Needle 334 may be a cylindrical flow passage that may be disposed outside of housing structure 335. The first end 3341 of the needle 334 communicates with the interior of the housing structure 335 and the second end 3342 of the needle 334 communicates directly with the environment.

The flow control structure 336 may include a sealing plug 3361 and a sealing plug displacement device. The plug displacement means may control the displacement of the sealing plug 3361 to control the opening and closing of the third flow path 333 and the needle 334. For example, the sealing plug 3361 may move to block the first end 3341 of the needle 334, or the sealing plug 3361 may move out of the blocking position, allowing the third flow passage 333 to communicate with the needle 334.

Illustratively, the plug displacement apparatus may include a connector 3362, a body 3363. The end of the connector 3362 may be provided with a sealing plug 3361. Axial movement of the coupling member 3362 may be accomplished by electromagnetic forces or the like to urge the sealing plug 3361 against or away from the first end 3341 of the needle 334 to effect blocking or unblocking. The connecting member 3362 may be a push rod, a spring, or the like.

This application is through sealing plug control flow channel break-make, realizes the regulation of pressure through the air source controller, compares in not having physics on-off control, only relies on atmospheric pressure control liquid blowout whether, and this application embodiment physically has controlled the flow channel break-make, makes the liquid spray volume more accurate.

It will be appreciated that providing the plug displacement means in an axial position can provide space savings. However, in other embodiments of the present application, the plug displacement means may not be provided on the axis. For example, the flow passage structure may be adjusted to provide the plug displacement means on the side. Of course, the plug displacement means may be other structures that function to allow the sealing plug 3361 to block the needle 334, and the application is not limited to this specific structure.

Baffle structures may also be provided in the housing structure 335. For example, the baffle structure may include a first baffle 3371 and a second baffle 3372.

The first baffle 3371 may be located at the deflected second end 3332 of the third flow channel 333. The diameter of the sealing plug 3361 may be greater than the diameter of the coupling 3362 and the first stop 3371 may limit the axial travel of the sealing plug 3361 after the sealing plug 3361 moves away from the needle 334.

In addition, when the droplet module 3 sucks liquid, the liquid in the needle 334 may not be sucked into the third flow channel 333 in time and may be retained in the housing structure 335. The first baffle 3371 can block these stagnant liquids, thereby making the liquids enter the third flow channel 333 as much as possible, improving the sensitivity of liquid control and improving the accuracy.

More specifically, even if liquid is not completely introduced into the third flow channel 333 at the time of liquid suction, the liquid overflows at most into a small space surrounded by the connection member 3362, the sealing plug 3361, the deflected second end 3332 of the third flow channel 333, and the first baffle 3371.

Preferably, the deflected second end 3332 of the third flow passage 333 is sealed off from the position where it passes through the first baffle 3371. Preferably, a seal is formed between the first barrier 3371 and the connection member 3362. For example, a sealing member may be disposed between the first barrier 3371 and the connection member 3362, or the first barrier 3371 may be made of or include a material that is easily sealed with the connection member 3362, such as rubber, silicon gel, polytetrafluoroethylene (PTFE), and the like.

The first baffle 3371 may be provided with a hollow hole to realize splicing with the third flow channel 333, and the first baffle 3371 may be a split structure located at the inner side and the outer side of the third flow channel 333; or the baffle and the flow channel can be integrally manufactured in a 3D printing mode.

Similarly, the second baffle 3372 may be provided with a hollow hole to realize splicing with the third flow channel 333, and the second baffle 3372 may be a split structure located on the inner side and the outer side of the third flow channel 333. The second stop 3372 may be used to fix the position of the body 3363 of the plug displacement apparatus, for example, the body 3363 may be fixed to the second stop 3372 by gluing or the like.

Referring to fig. 5, needle 334 may include a flow path wall 3343 and a flow path 3344 located in flow path wall 3343. The end of the flow channel wall 3343 (e.g., the lower end of fig. 4) may or may not be beveled, resulting in a truncated cone or cylinder at the end. In a cross-sectional view along the axis of the needle 334, the angle between the waist line L1 (or generatrix) of the flow channel wall 3343 and the axis L2 is α, which satisfies: alpha is less than or equal to 30 degrees. The flow path 3344 may be circular in cross-section.

The diameter of the flow channel 3344 is R1, and the diameter of the end face of the flow channel wall 3343 is R2, and the results of the experiment are shown in the following table.

Table 1:

table 2:

table 3:

table 4:

table 5:

table 6:

as can be seen from tables 1 to 4, when the other parameters are not changed, the smaller the angle α between the waist line L1 of the flow channel wall 3343 and the axis L2 is, the smaller the variation coefficient of the liquid discharge amount is, and the more stable the liquid discharge amount of the needle 334 is. When the included angle alpha is less than or equal to 30 degrees, the technical effect of ensuring that the coefficient of variation does not exceed 15 percent is achieved. Preferably, when the included angle α is less than or equal to 10 °, the technical effect is that the coefficient of variation does not exceed 10%.

As can be seen from any of tables 1 to 4, the smaller the air pressure is, the smaller the discharge amount (droplet volume) of the needle 334 is, while the other parameters are unchanged. That is, the air pressure can be controlled by controlling the air source controller 32, so that the micro sample application can be realized.

As can be seen from table 5, when the other parameters are not changed, the smaller the diameter R1 of the flow path 3344, the smaller the coefficient of variation of the discharged liquid amount, which indicates that the discharged liquid amount from the needle 334 is more stable.

As can be seen from table 6, when the other parameters are not changed, the smaller the diameter R2 of the end surface of the flow channel wall 3343, the smaller the coefficient of variation of the discharged liquid amount, which means that the discharged liquid amount from the needle 334 is more stable.

The diameter R1 of the flow path 3344 of the present application may satisfy: r1 is more than or equal to 130 mu m and less than or equal to 250 mu m. The diameter R2 of the end face of the flow channel wall 3343 may satisfy: r2 is more than or equal to 350 mu m and less than or equal to 700 mu m.

It is understood that the printing type of the present application is non-contact printing. Compared with the traditional contact type sample application, the liquid drop output is stable, the variation coefficient is small, and the amount of the reagent which can be carried by the needle head is relatively large. Compared with the traditional injection pump, the liquid outlet quantity can be smaller through air pressure control, and more use scenes are met.

Referring to fig. 2, the detection module 4 may include a detection camera 41 and a fill-in light device 42. Detection camera 41 may be tilted and aimed at needle 334. The fill-in light device 42 may be a fill-in light or the like, and provides a sufficient light source for the detection camera 41. The fill-in light device 42 may be disposed at the opposite side of the detection camera 41, i.e., in the horizontal direction, and a needle 334 (not shown in fig. 2) is disposed at a middle position of the detection camera 41 and the fill-in light device 42.

The detection module 4 and the liquid drop module 3 can be connected together to realize synchronous movement.

In the traditional scheme, detect the camera and set up usually in frame construction's top, after 3 spotting of liquid drop module, through the size, the distribution that detect the camera and detect the liquid drop, judge whether the spotting meets the requirements. The position is fixed, for example, after the needle head samples on the chip, the detection camera can take pictures to judge whether the sample application result meets the processing requirement, and the efficiency is low. For example, if there are 10 spotting operations, the 5 th spotting is not qualified, but the 10 th spotting is finished to judge whether it is qualified or not, which wastes time and materials and is not efficient.

And the detection camera 41 in this application can move with the liquid drop module 3 synchronous, and its focus can be fixed, can realize the continuation of spotting with detecting and go on, and efficiency is higher. For example, 10 sample application operations are integrally performed, the sample application at the 5 th position is unqualified, the sample application at the 5 th position can be found out unqualified through the detection module 4, and an operator can be reminded or other mechanical arms can be controlled to discard unqualified chips in sample application by matching with corresponding programs, so that the efficiency is improved, and materials are saved.

The detection camera 41 may be broadly understood as a device that can recognize images.

Referring to fig. 2, the reagent module 5 may be a structure for storing and holding a reagent. The liquid module 3 can absorb liquid from the reagent module 5 and move to the sample application station module 6 and the like to realize subsequent processes.

Referring to fig. 2, 6, 7, the spotting station module 6 may comprise a first base 61, a base post 62, and a second base 63. The second base 63 is attached to the base plate 11 of the frame 1. And, the second base 63 supports the first base 61 through the base pillar 62.

The first base 61 is provided therein with a mounting groove 611. Illustratively, taking a chip in which a product to be spotted is rectangular as an example, the mounting groove 611 may be substantially rectangular. At least one of four corners of the mounting groove 611 may be provided with a first redundant space 612, and at least one of two pairs of opposite sides of the mounting groove 611 may be provided with a second redundant space 613. Illustratively, the first redundant space 612 and the second redundant space 613 may be spaces beyond the rectangular sides of the mounting groove 611. As shown in fig. 7, an edge of the first redundant space 612 and the second redundant space 613 may be arc-shaped. The first redundant space 612 may be a 3/4 circle and the second redundant space 613 may be a semicircle.

The first redundant space 612 prevents a right angle of a product, such as a rectangle, from being attached to a right angle of the mounting groove 611, and prevents a force between the product and the mounting groove 611 from damaging the product when the product is placed and taken. The second redundant space 613 provides an operating space for placement and access of products. For example, tweezers and clamping jaws may be inserted into the second redundant space 613 to perform clamping and the like. Based on the function of the redundant space, the area of the second redundant space 613 may be larger than that of the first redundant space 612, which is more favorable for the clamping operation.

In the conventional scheme, when the installation groove 611 is smooth or liquid such as water exists in the installation groove 611, the problem that the product is difficult to take can occur after the product is installed in the installation groove 611. In the embodiment of the present application, a through hole 614 penetrating the first base 61 in the height direction may be provided in the mounting groove 611. The through holes 614 can reduce the pressure of vacuum suction on one hand, and can provide a force application site on the other hand, for example, the product can be pushed upwards from the through holes 614 at the bottom of the first base 61 by using related tools, and then the product can be gripped from above by cooperating with tools such as tweezers and clamping jaws, thereby facilitating the taking of the product. The damage of the product caused by small contact area and large clamping force when the product is directly taken from the upper part is prevented.

Of course, the application does not limit the number of mounting slots 611 provided in the spotting station module 6. For example, a plurality of mounting grooves 611 may be provided so that printing can be performed simultaneously for a plurality of products. The base 62 may be connected to the first base 61 and the second base 63 by, for example, screw threads. It can be appreciated that the present application provides increased flexibility in the manner of removably attaching the base to the base, allowing for the replacement of the first base 61 with the corresponding mounting slot 611, depending on the desired product application.

Referring to fig. 6, 8, 9, 10, the wash module 7 may include a wash zone 71 and a dry zone 72.

The cleaning section 71 may include a cleaning tank 711, a cleaning column 712, and a connection block 713. The cleaning tank 711 may be connected to the connection block 713, and mounted to the substrate 11 of the frame 1 through the connection block 713.

The cleaning column 712 is disposed in the cleaning tank 711. The cleaning column 712 can controllably eject liquid, for example, the needle 334 of the droplet module 3 can be moved to clean after one spotting operation is finished, so as to perform the next round of liquid extraction, spotting and cleaning operations.

Illustratively, a cleaning hole 7121 may be provided in the cleaning column 712, wherein the cleaning hole 7121 can vertically spray the cleaning liquid, and the spraying height of the cleaning liquid is higher than the highest point of the cleaning column 712. The number of the cleaning holes 7121 can be one or more, for example, three cleaning holes 7121 are arranged in fig. 8, and the simultaneous cleaning of three needles can be realized. Of course, the present application does not limit the number of the cleaning holes 7121.

The top surface of the cleaning column 712 may be provided with a return 7122, and both sides of the return 7122 may include surfaces with different degrees of inclination, such as a horizontal surface 7123 and a slanted surface 7124. The horizontal face 7123 may be parallel to the substrate 11 and the inclined face 7124 may be inclined to the substrate 11. Also, the height of the inclined surface 7124 gradually decreases as it goes away from the bent portion 7122. The bent portion 7122 may pass through the center of the cleaning hole 7121.

The inclined face 7124 can reduce the retention of the cleaning solution on the top surface of the cleaning column 712 and guide the flow of the cleaning solution. Referring to fig. 9, a discharge port 7111 may be provided at a bottom position of the cleaning tank 711 on the side of the inclined surface 7124 (i.e., a bottom portion of the cleaning tank) so that the cleaning solution can be smoothly discharged. Referring to fig. 6, ramp 7124 may face away from the side of the spotting station module 6, trying to avoid splashing of cleaning solution onto the spotting station module 6.

The horizontal surface 7123 can maintain the flushing height of the liquid in the cleaning holes 7121. For example, if the washing column 712 is provided as an integral inclined surface under the same pressure, the height of the solution sprayed out is limited. To ensure that needle 334 is cleaned, needle 334 needs to extend into cleaning aperture 7121 to some extent, for example, needle 334 is below the highest point of cleaning aperture 7121. However, corrosive and diffusive substances may remain on the spotted needle 334, and if the needle 334 extends into the cleaning hole 7121, the corrosive and diffusive substances are likely to diffuse into the supply source of the cleaning solution through the cleaning hole 7121, thereby causing cross contamination. The horizontal plane 7123 in the present application can maintain the flushing height of the cleaning solution, and the needle 334 can be located above the horizontal plane 7123 (or the highest point of the cleaning column 712), and is cleaned by the sprayed water column, so as to avoid the problem of cross contamination as much as possible.

The cleaning module 7 includes a cleaning solution supply source connected to the cleaning hole 7121. A check valve (not shown) may be provided between the cleaning solution supply source and the cleaning holes 7121 to prevent cross contamination.

An arc face 7125 can be arranged on one side of the cleaning column 712 far away from the discharge port 7111, an inclined face can be arranged at the bottom of the cleaning tank 711, and the discharge port 7111 is the lowest point of the bottom of the cleaning tank 711, so that waste liquid can be timely recovered.

Referring to fig. 6, 10, the drying zone 72 may include a drying block 721, a base 722, and a guide 723. The drying zone 72 may be disposed between the wash tank 711 and the sample station module 6. The drying block 721 may be, for example, a dust-free cotton.

The sample application station module 6 and the cleaning tank 711 are spaced from each other by a certain distance, so that liquid can be prevented from splashing to the sample application station module 6 in the cleaning process. And a drying area 72 is arranged between the spotting station module 6 and the cleaning tank 711, so that the module is compact as a whole, splashed liquid can be absorbed to a certain degree, and the possibility of pollution is reduced. After cleaning needle 334, needle 334 may be extended into drying block 721 to dry needle 334 as much as possible.

A guide 723 may be provided on the substrate 11, a base 722 slidably mounted on the guide 723, and a drying block 721 connected to the base 722. So that the base is easily drawn and the drying block 721 is easily replaced. The drying block 721 may include a housing and a core, and the housing may be provided with a magnet to be adsorbed and fixed to the cleaning tank 711 or the spotting station module 6, thereby reducing shaking.

While the foregoing is directed to the preferred embodiment of the present application, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the application.

In addition, the above-mentioned liquid is to be broadly understood in the present application, and examples of the liquid include a solution, a suspension, an emulsion, a dispersion, a melt, and water.

The embodiments of the present application disclose the following aspects.

The pipetting spotting station comprises:

a substrate;

the motion module is arranged on the base plate and comprises a guide rail and a sliding block, and the sliding block can move in the three directions X, Y, Z which are perpendicular to each other;

a droplet module comprising a needle in communication with an external environment, the droplet module configured to control the needle to aspirate and eject liquid by controlling air pressure; and

the detection module is connected with the liquid drop module in the motion module through the same sliding block, so that the liquid drop module and the detection module can move synchronously, the detection module comprises a detection camera and a light supplement device, the detection camera can shoot liquid sprayed out of the needle head, and the needle head is located between the detection camera and the light supplement device in the horizontal direction.

In at least one embodiment, the needle comprises a flow passage wall and a flow path in the flow passage wall, the flow passage wall having a waistline in a cross-section along an axis of the needle at an angle α to the axis such that: alpha is less than or equal to 30 degrees, and the cross section of the flow path is circular.

In at least one embodiment, α ≦ 10.

In at least one embodiment, the diameter of the flow channel is R1, and the diameter of the end surface of the flow channel wall is R2, satisfying:

130μm≤R1≤250μm；

350μm≤R2≤700μm。

in at least one embodiment, the droplet module further comprises:

the syringe comprises a shell structure, a needle head and a needle seat, wherein a flow passage is arranged in the shell structure, and one end of the flow passage is connected with the needle head in an on-off controllable manner; and

the flow passage control structure comprises a sealing plug, and the flow passage control structure controls the on-off of the flow passage and the needle head by controlling the sealing plug to block or not to block the connecting position of the needle head and the flow passage.

In at least one embodiment, further comprising a cleaning module comprising a cleaning zone,

the washing area includes:

the cleaning tank is provided with a discharge port for discharging liquid at the bottom;

In at least one embodiment, the slope has a height gradually decreasing as it goes away from the turn, and the slope is closer to the discharge port than the horizontal plane in a horizontal direction.

This cleaning module for syringe needle includes the washing district, the washing district includes:

the cleaning column is arranged in the cleaning tank, one or more cleaning holes are formed in the cleaning column, cleaning liquid can be vertically and upwards sprayed out of the cleaning holes, the spraying height of the cleaning liquid is larger than the highest point of the cleaning column,

the top surface of the cleaning column comprises two surfaces with different inclination degrees, a turning part is arranged between the two surfaces with different inclination degrees, and the turning part penetrates through the circle center of the cleaning hole.

In at least one embodiment, the slope is closer to the discharge port than the horizontal plane in a horizontal direction.

In at least one embodiment, the side of the washing column remote from the discharge opening is provided with an arc-shaped face.

In at least one embodiment, the bottom inner surface of the cleaning tank is an inclined surface, and the discharge port is located at the lowest point of the bottom inner surface of the cleaning tank.

In at least one embodiment, the cleaning module for a needle comprises a cleaning liquid supply source connected to the cleaning hole, and a check valve is disposed between the cleaning liquid supply source and the cleaning hole.

In at least one embodiment, the needle wash module further comprises a drying zone comprising a drying block, a base, and a guide,

the base is slidably mounted on the guide rail, the drying block is connected to the base, and the drying block is abutted to the cleaning tank.

The application provides a pipetting and spotting workstation comprising a washing module as described above; and

a droplet module capable of sucking and ejecting liquid, the droplet module including a needle,

when the needle moves to the cleaning hole to be cleaned, the needle is positioned above the highest point of the cleaning column.

In at least one embodiment, the pipetting station comprises a wash module as described above; and

a sample application station module, the drying zone being located between the sample application station module and the cleaning zone.

The liquid drop module is used for sucking and ejecting liquid, the liquid drop module comprises a gas source controller and a liquid drop module main body, the liquid drop module main body is connected with a gas source through the gas source controller,

the liquid drop module body comprises a needle head which is communicated with the external environment, the liquid drop module is arranged to control the needle head to suck and eject liquid by controlling air pressure,

the needle comprises a flow channel wall and a flow path positioned in the flow channel wall, in a section along the axis of the needle, the included angle between the waist line of the flow channel wall and the axis is alpha, and the alpha satisfies the following conditions: alpha is less than or equal to 30 degrees.

In at least one embodiment, the droplet module further comprises:

the needle head comprises a shell structure, wherein a flow passage is arranged in the shell structure, one end of the flow passage is connected to the air source controller, and the other end of the flow passage is connected to the needle head in a switching and controllable mode; and

the flow passage control structure comprises a sealing plug, and the flow passage control structure controls the connection position of the needle head and the flow passage to control the on-off of the other end of the flow passage and the needle head by controlling the sealing plug to block or not to block the needle head.

In at least one embodiment, the flow channel comprises:

one end of the first flow channel is connected to the air source controller;

the cross section of the second flow channel is a circular ring, and one end of the second flow channel is connected to the other end of the first flow channel;

the first end of the third flow passage is of a hollow cylinder, the first end of the third flow passage is connected to the other end of the second flow passage, the second end of the third flow passage is deflected inwards along the radial direction of the second end of the third flow passage, the cross section of the second end of the third flow passage is a circular ring, and when the sealing plug is not blocked, liquid can flow through the second end and the needle head.

In at least one embodiment, the flow control structure further comprises a sealing plug displacement device comprising a connector and a body, the sealing plug being arranged at an end of the connector, the connector being movably connected to the body, the body being arranged to enable axial movement of the connector,

the sealing plug displacement device and the sealing plug are arranged in a central cavity of the third flow passage, and when the sealing plug is pushed by the connecting piece to abut against the needle head, the sealing plug blocks the communication between the third flow passage and the needle head.

In at least one embodiment, a baffle structure is provided in the housing structure, the baffle structure including a first baffle plate having a hole therein through which the third flow passage and the connector pass, the sealing plug having a diameter greater than a diameter of the connector,

after the sealing plug moves away from the needle, the sealing plug abuts against the first baffle, and a sealing space is formed between the first baffle and the needle.

In at least one embodiment, a baffle structure is disposed in the housing structure, the baffle structure including a second baffle disposed at a junction of the second flow passage and the third flow passage, the main body of the sealing plug displacing device being connected to the second baffle.

In at least one embodiment, α ≦ 10.

130μm≤R1≤250μm；

350μm≤R2≤700μm。

the application provides a move liquid sample application workstation includes:

a droplet module as described above; and

a cleaning module comprising a cleaning zone,

the washing area includes:

a cleaning module capable of cleaning the needle of the droplet module.

In at least one embodiment, the wash module comprises a wash zone comprising:

a cleaning tank in which a discharge port for discharging liquid is provided;

In at least one embodiment, the height of the slope gradually decreases away from the inflection.

In at least one embodiment, the cleaning module includes a drying section including a drying block, a base, and a guide rail, the base is slidably mounted to the guide rail, the drying block is connected to the base, and the drying block abuts against the cleaning tank.

Claims

1. The utility model provides a sample application station module, its characterized in that, includes first base, be provided with the mounting groove in the first base, be provided with in the mounting groove run through on the thickness direction of first base the through-hole of first base, the mounting groove is the rectangle, at least one corner in the four corners of mounting groove is provided with first redundant space.

2. Printing station module according to claim 1, characterised in that at least one of the two pairs of opposite sides of the mounting groove is provided with a second redundant space, respectively.

3. Printing station module according to claim 1, further comprising a base post and a second base, the first base being detachably connected to the second base by the base post.

4. A pipetting spotting station, comprising:

the deposition station module of any one of claims 1 to 3;

a cleaning module capable of cleaning the needle of the droplet module.

5. Pipetting deposition station according to claim 4, wherein the wash module comprises a wash zone comprising:

a cleaning tank in which a discharge port for discharging liquid is provided;

6. A pipetting spotting station as recited in claim 5 wherein the top surface of the washing column comprises two surfaces with different degrees of tilt with a turn between them that passes through the center of the washing well.

7. Pipetting deposition station according to claim 6, characterized in that the two faces differing in the degree of inclination comprise a horizontal face and a slanted face.

8. Pipetting deposition station according to claim 7, characterized in that the height of the ramp decreases gradually away from the turn.

9. Pipetting station according to claim 7, characterized in that the ramp is further from the station module than the horizontal plane.

10. Pipetting station according to claim 4, characterized in that the needle comprises a channel wall and a flow path in the channel wall, the waist line of the channel wall in a cross section along the axis of the needle making an angle α with the axis, α satisfying: alpha is less than or equal to 30 degrees, and the cross section of the flow path is circular.