CN218628709U - Temperature detection device and nucleic acid purification and amplification system - Google Patents

Abstract

The application discloses temperature-detecting device and nucleic acid purification amplification system relates to the temperature detection technology field, be used for detecting the temperature, be difficult to adapt to the problem of different occasion temperature measurement demands to traditional temperature-detecting device, a temperature-detecting device is provided, including thermistor, platinum thermistor, temperature sensor such as thermocouple, be connected with the controller through the temperature acquisition circuit, make the technical staff can select suitable temperature sensor to carry out temperature detection according to the operating condition needs, the temperature-detecting device that this application provided can be adapted to more complicated manifold operating mode, satisfy the temperature measurement demand of different occasions. Meanwhile, the temperature measurement environment and the temperature measurement precision of the multiple temperature sensors are different, and the temperature sensors are applied together to meet different temperature measurement requirements, so that the application scenes of the temperature detection device are further widened.

Description

Temperature detection device and nucleic acid purification and amplification system

Technical Field

The application relates to the technical field of temperature detection, in particular to a temperature detection device and a nucleic acid purification and amplification system.

Background

In the industrial production process, environmental factors and a plurality of production processes can cause temperature changes, the temperature changes have great influence on the performance of various electronic equipment, the monitoring effect of temperature parameters directly influences the production efficiency and the production safety, and the precision and the sensitivity of the temperature detection device play a vital role in the development of the industrial field.

At present, common temperature sensors comprise thermistors, platinum thermistors, thermocouple sensors and the like, and different temperature sensors are selected as means for detecting temperature according to different use requirements. However, the sensor used by the conventional temperature detection device is single at present, and is difficult to adapt to the temperature measurement requirements of different occasions.

Therefore, those skilled in the art need a temperature detection device to solve the problem that the conventional temperature detection device is difficult to adapt to the temperature measurement requirements of different occasions.

SUMMERY OF THE UTILITY MODEL

The application aims at providing a temperature detection device and a nucleic acid purification and amplification system to solve the problem that the traditional temperature detection device is difficult to adapt to temperature measurement requirements of different occasions.

In order to solve the above technical problem, the present application provides a temperature detection device, including:

the temperature acquisition circuit, the controller, the thermistor sensor, the platinum thermistor sensor and the thermocouple sensor;

the controller is connected with the thermistor sensor, the platinum thermistor sensor and the thermocouple sensor through the temperature acquisition circuit.

Preferably, the temperature calibration device further comprises a memory for storing the temperature calibration parameters, and the memory is connected with the controller.

Preferably, the system further comprises a communication module connected with the controller, and the communication module is provided with a TTL interface and an RS-232 interface.

Preferably, the thermistor sensor, the platinum thermistor sensor and the thermocouple sensor are connected with the temperature acquisition circuit through interfaces, and the temperature acquisition circuit is further provided with an expansion interface.

Preferably, the thermistor sensor, the platinum thermistor sensor and the thermocouple sensor are plural.

Preferably, the specifications of the different thermistor sensors, platinum thermistor sensors or thermocouple sensors are different.

Preferably, a DC-DC converter is further included between the external power source and the controller.

Preferably, the power supply further comprises a reverse connection preventing circuit arranged between the power supply and the DC-DC converter.

Preferably, the reverse connection preventing circuit includes: the device comprises an NMOS tube, a parasitic diode, a first voltage-dividing resistor, a second voltage-dividing resistor and a first capacitor;

the grid electrode of the NMOS tube is connected with the negative electrode of a power supply, the source electrode of the NMOS tube is connected with the positive electrode of the power supply through a first divider resistor, and the drain electrode of the NMOS tube is connected with the negative electrode output end of the DC-DC converter;

the parasitic diode, the second voltage-dividing resistor and the first capacitor are connected in parallel with the grid and the source of the NMOS tube; the anode of the parasitic diode is correspondingly connected with the source electrode of the NMOS tube.

Preferably, the crystal oscillator circuit of the controller is provided with a plurality of crystal oscillators with different frequencies, each crystal oscillator is connected to the crystal oscillator circuit through a selector switch, and a control end of the selector switch is connected with the controller.

Preferably, the temperature acquisition circuit includes:

a filter circuit disposed between the thermistor sensor and the controller;

and/or: a voltage stabilizing circuit arranged between the thermistor sensor and the controller;

and/or: a differential circuit disposed between the platinum thermistor sensor and the controller;

and/or: a transient suppression circuit disposed between the thermocouple sensor and the controller.

In order to solve the above technical problem, the present application further provides a nucleic acid purification and amplification system, including the above temperature detection device and a nucleic acid purification and amplification instrument, the nucleic acid purification and amplification instrument includes: a nucleic acid amplification module and a nucleic acid purification module.

Preferably, the thermistor sensor of the temperature detection device is an NTC thermistor sensor, the platinum thermistor sensor is a PT1000 sensor, and the thermocouple sensor is a K-type thermocouple sensor;

the NTC thermistor sensor is arranged at an amplification plate of the nucleic acid amplification module;

the PT1000 sensor is arranged at a Peltier position of the nucleic acid amplification module;

the type K thermocouple sensor is disposed at an amplification plate of the nucleic acid amplification module.

Preferably, the thermistor sensors of the temperature detecting device are NTC thermistor sensors, and at least two of them are respectively disposed at the amplification plate of the nucleic acid amplification module and on the upper surface of the peltier element of the nucleic acid amplification module.

The application provides a pair of temperature-detecting device, including temperature sensor such as thermistor, platinum resistance, thermocouple, be connected with the controller through temperature acquisition circuit for the technical staff can select suitable temperature sensor to carry out temperature detection according to operating condition needs, makes temperature-detecting device be adapted to more complicated manifold operating mode, satisfies the temperature measurement demand of different occasions.

The nucleic acid purification and amplification system provided by the application corresponds to the temperature detection device and has the same effect.

Drawings

In order to more clearly illustrate the embodiments of the present application, the drawings required for the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and that other drawings may be obtained by those skilled in the art without inventive effort.

Fig. 1 is a structural diagram of a temperature detecting device provided by the present invention;

fig. 2 is a circuit diagram of a memory and its related circuits provided by the present invention;

fig. 3 is a circuit diagram of a communication module and a related circuit thereof according to the present invention;

fig. 4 is a circuit diagram of a DC-DC converter and its related circuits provided by the present invention;

fig. 5 is a circuit diagram of an anti-reverse connection circuit provided by the present invention;

fig. 6 is a circuit diagram of a filter circuit between an anti-reverse connection circuit and a power supply provided by the present invention;

fig. 7 is a schematic diagram of a temperature acquisition result of a K-type thermocouple provided by the present invention;

fig. 8 is a schematic diagram of a PT1000 temperature acquisition result provided by the present invention;

fig. 9 is a schematic diagram of NTC temperature acquisition results provided by the present invention.

The temperature acquisition circuit 11 is a temperature acquisition circuit, the controller 12 is a thermistor sensor 13, the platinum thermistor sensor 14 is a thermocouple sensor 15, the memory 16 is a communication module 17, and the upper computer 18 is a computer.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all the embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without any creative effort belong to the protection scope of the present application.

The core of the application is to provide a temperature detection device and a nucleic acid purification and amplification system.

In order that those skilled in the art will better understand the disclosure, the following detailed description will be given with reference to the accompanying drawings.

In the current industrial production, a commonly used temperature detection device usually selects a temperature sensor with more appropriate performance parameters as a device for acquiring temperature information according to the working environment of the temperature detection device, but when the temperature measurement environment is more complex and the temperature measurement points are more, the temperature detection device using a single sensor cannot well meet the temperature measurement requirement, and the whole equipment becomes overstaffed by using a plurality of different temperature detection devices. In order to solve the above problem, the present application provides a temperature detection apparatus, as shown in fig. 1, including:

the temperature acquisition circuit 11, the controller 12, the thermistor sensor 12, the platinum thermistor sensor 13 and the thermocouple sensor 14;

the controller 12 is connected with a thermistor sensor 13, a platinum thermistor sensor 14 and a thermocouple sensor 15 through a temperature acquisition circuit 11.

For the implementation of the Controller 12, the specific type and kind of the Controller 12 are not limited in this embodiment, and the Controller 12 may be implemented by a Single-Chip Microcomputer (Single-Chip Microcomputer), a Programmable Logic Controller (PLC), and other devices. In one possible implementation, the controller 12 is a main control chip STM32F407ZETG and an operating circuit thereof, so as to achieve the effect of reducing power consumption.

It is easy to know that the control device such as a single chip is usually equipped with the supporting circuit of the minimum system, as for the application scenario of the above embodiment that uses the main control chip STM32F407ZETG as the device for implementing the controller function.

The minimum system is preferably provided with a reset button which can carry out one-key reset; preferably, the electrostatic protection device is provided with an electrostatic diode for electrostatic protection; the design of crystal oscillator circuit supports inside crystal oscillator and outside crystal oscillator automatic switching function, and the accessible change over switch switches the connection crystal oscillator as master control chip STM32F407ZETG clock signal input, realizes different operating frequency's switching, adapts to the needs to different reaction rates under the different application scenes better, is applicable to real-time temperature and gathers, and temperature acquisition frequency is high.

In consideration of temperature measurement precision and usability, the temperature detection device selects the contact temperature sensors as a mode for acquiring temperature information, comprehensively considers the performance parameters and application scenes of the temperature sensors in a plurality of contact temperature sensors, and selects three temperature sensors, namely a thermistor sensor 13, a platinum thermistor sensor 14 and a thermocouple sensor 15.

The thermistor is a commonly used temperature sensor, and has the characteristics that the resistance value of the thermistor is reduced in an exponential relation along with the temperature rise, the thermistor is sensitive to the temperature, but due to the nonlinearity of the thermistor, the consistency of components is poor, and the thermistor is more suitable for carrying out high-precision temperature measurement in a narrow temperature change range. The thermistor is divided into two types, namely, a Positive Temperature Coefficient thermistor (PTC) and a Negative Temperature Coefficient thermistor (NTC), according to the difference of Temperature coefficients, and the NTC thermistor is generally used.

Unlike a thermistor, the resistance of a platinum thermistor increases linearly with the temperature rise, and conventional platinum thermistors include PT100 and PT1000, which are distinguished in that the resistance of PT100 is 100 Ω at 0 ℃, 138.5 Ω at 100 ℃, 1000 Ω at 0 ℃ and 1385.1 Ω at 100 ℃, and thus the PT1000 precision is one order of magnitude higher than PT 100. The temperature measuring range of PT1000 is 0-300 deg.C, and the temperature measuring range of PT100 is-200-650 deg.C. In practical application, the corresponding platinum thermal resistor can be selected according to the temperature measurement range and the precision requirement. However, the sensing of the platinum thermistor to temperature is represented by a variable resistance value, and reading the resistance value requires applying a voltage, and cannot measure a transient temperature change, so that the platinum thermistor needs to be used in combination with other sensors.

The thermocouple sensor does not need power supply excitation, the temperature measuring range is larger, the most common K-type thermocouple temperature measuring range is-200-1200 ℃ according to different types. However, the temperature measurement principle determines that the thermocouple sensor must perform cold end temperature compensation, and one more error point will seriously affect the temperature measurement accuracy, so the thermocouple sensor is more suitable for being applied to temperature measurement scenes with large range and low accuracy requirement.

It is easy to understand that, the above-mentioned three kinds of temperature sensor that choose for use this application does not limit its quantity, and each kind of temperature sensor all can be one or more, and same kind of temperature sensor sets up a plurality ofly can satisfy the demand of carrying out the temperature measurement to a plurality of temperature measurement points in the same temperature measurement scene. In one possible embodiment, for example, there are 10 corresponding NTC thermistors, 10 PT1000 platinum thermistors, 16K-type thermocouples, and 36 temperature collecting channels of the temperature detecting device.

Furthermore, different individuals of the same temperature sensor have different specifications to adapt to different temperature measurement needs, for example, to the temperature measurement of a part of narrow gap, a temperature sensor with a smaller volume should be selected, so that the temperature sensor can stretch into the narrow gap to be in direct contact with the narrow gap, and accurate temperature measurement is carried out. Similarly, for each temperature measuring point with different shapes and sizes, the temperature measuring requirements of the temperature measuring points are met by using the temperature sensors with different specifications.

It should be noted that, the connection manner between the temperature acquisition circuit 11 and the temperature sensor is not limited in this embodiment. In order to ensure the miniaturization and integration of the temperature detection device, the temperature acquisition circuit 11 is usually integrated on a circuit board, and the temperature acquisition circuit 11 and each temperature sensor may be connected in a direct connection manner or in an interface manner. The detachable temperature sensor is supported by adopting a connection mode of an interface, and the interface can adopt universal interface specifications such as an I2C (Inter-Integrated Circuit), a System Management Bus (SMBus), a Serial Peripheral Interface (SPI) and the like so as to support the use and replacement of temperature sensors with different models and specifications.

Similarly, the connection relationship between the circuit board (collection board) integrated with the temperature collection circuit 11 and the controller 12 can be connected in a detachable manner such as an interface, and a plurality of collection boards can be connected simultaneously by using the idle pin resources of the controller 12, so as to further expand the temperature collection channel.

In addition, in order to improve the universality of the temperature detection device provided by the application, an optimal embodiment is that the temperature acquisition circuit 11 is further provided with an expansion interface, and the universal interface specification can be adopted, so that the expansion of the temperature acquisition channel of the device can be realized only by accessing other temperature sensors meeting the corresponding interface specification.

The temperature detection device provided by the application can meet the temperature measurement requirements under different temperature measurement scenes through three temperature sensors, namely the thermistor sensor 13, the platinum thermistor sensor 14 and the thermocouple sensor 15. Each temperature sensor can be selected for use a plurality of temperature measurement points to meet more diversified temperature measurement requirements, and the temperature sensor is connected with the temperature acquisition circuit 11 through a universal and standard interface so as to be convenient for replacement. Different individuals of the same temperature sensor can adopt different specifications so as to meet the temperature measurement requirements of the surfaces to be measured with different shapes. The temperature detection device that this application provided can use in the temperature measurement scene of difference, through the temperature sensor pertinence that uses different types, specification satisfy the temperature measurement demand of differentiation, the setting of multichannel temperature acquisition passageway also is favorable to carrying out the temperature detection simultaneously to a plurality of temperature measurement points, to same object of awaiting the temperature measurement, also can compare the check-up through a plurality of temperature sensors, acquires more accurate temperature measurement result.

Further, in addition to the description of the modules and devices included in the temperature detection apparatus in the above embodiment, this embodiment also provides a preferred embodiment, as shown in fig. 1, the temperature detection apparatus further includes:

a memory 16 storing temperature calibration parameters, the memory 16 being connected to the controller 12.

It should be noted that, in this embodiment, the memory 16 may use any storage medium, and stores the temperature calibration parameters, so that the controller 12 can achieve the effect of calibrating the temperature information acquired by each temperature acquisition channel, so as to improve the accuracy of temperature acquisition.

But preferably a storage medium that supports data writing and erasing, such as that shown in fig. 2, in one possible implementation, the memory 16 is a W25Q128FV chip, and a portion of the circuitry associated therewith, as shown in fig. 2, includes: memory chip U42, capacitor C43. The W25Q128FV chip is an EEPROM (Electrically Erasable Programmable read only memory) chip, which is a memory chip with no data loss after power failure and supports multiple writing and erasing in operation. Meanwhile, the memory chip has small working current and low power consumption, is not easy to generate heat, and is more suitable for the application requirement of an actual temperature detection device.

In addition, the temperature calibration parameters stored in the memory 16 may be calibration parameters for temperature measurement results of various temperature sensors obtained according to experimental data or historical temperature measurement data, the temperature calibration parameters may be modified, and related personnel may freely adjust the temperature calibration parameters according to actual temperature detection conditions, and write new temperature monitoring parameters into the memory 16 for modification of the calibration process.

It is understood that, in addition to storing the temperature calibration parameters in the memory 16, there is also a function of temporarily storing the temperature data, and the temperature information collected by each temperature collection channel can be temporarily stored in the memory 16 for being called by the external device or used by the controller 12.

Correspondingly, this embodiment also provides another preferable solution, as shown in fig. 1, the temperature detecting device further includes:

and the communication module 17 is connected with the controller 12, and the communication module 17 is provided with a TTL interface and an RS-232 interface.

The communication module 17 is used for communicating with the upper computer 18, the collected temperature information is sent to the upper computer 18 after being calibrated by the controller 12, the upper computer 18 obtains final temperature information to perform subsequent processes or steps, and in the application scenario, the controller 12 has a main function of correcting the temperature information collected by the temperature collection channel according to the temperature calibration parameters stored in the memory 16 to obtain more accurate temperature information for the upper computer 18.

As described in the foregoing embodiment, the TTL interface and the RS-232 interface of the communication module 17 are both widely used serial ports, and are used to implement data communication between devices.

TTL: also known as TTL levels, the interface protocol provides that +5V is equivalent to a logic "1" and 0V is equivalent to a logic "0" (when data is represented in binary).

RS-232: also known as an asynchronous transmission standard interface, is one of the commonly used serial communication interface standards.

It should be further noted that the communication distance between the RS-232 and TTL serial port supported by the communication module is long, which can meet some requirements for remote testing, and the upper computer 18 does not need to be deployed near the temperature detection device, i.e. the temperature detection device can be applied to some application scenarios with more complex working environments, thereby further improving the versatility of the device.

As can be seen from the above, the communication module 17, the TTL interface and the RS-232 interface are all configured to better implement communication between the temperature detection device and the upper computer 18, and improve compatibility of the device, so that interface conversion can be performed on the communication module 17 according to a communication interface protocol adopted by the upper computer 18. The TTL interface and the RS-232 interface used in the communication interface in the above embodiments are both common Serial communication protocols, and there are mature solutions or apparatuses for converting the above interface and other interface protocols, such as a Serial to Universal Serial Bus (USB), and the like, and the type of the interface of the communication module 17 compatible with the upper computer 18 can be extended by using such an interface conversion tool.

Similarly, the present embodiment also does not limit the implementation form of the communication module 17, but also provides a preferred embodiment, as shown in fig. 3, the communication module 17 is implemented by an SP3232EEN chip and its related circuits, including: a communication chip U22; light emitting diodes LED1, LED2; resistors R19, R20; capacitors C2, C3, C5, C10, C45. The device also has the advantages of small working current, low power consumption, difficult heating and the like.

The preferred scheme that this embodiment provided realizes the temporary storage to temperature information through adding memory 16 to the call of equipment such as follow-up host computer 18, and, memory 16 still can be used to the storage temperature calibration parameter, and controller 12 can calibrate the temperature information that obtains to temperature sensor collection according to the temperature calibration parameter in memory 16, in order to improve the temperature detection precision, realizes better temperature detection effect. And the communication with the upper computer 18 is realized by adding the communication module 17, so that the temperature information acquired by the temperature detection device can be used by other equipment, and more various functions can be realized, and in the application scene, the controller 12 only needs to calibrate the acquired temperature information according to the temperature calibration parameters stored in the memory 16, and other work can be finished by the upper computer 18 communicated with the controller, so that the running resources of the controller 12 are liberated, the performance requirement on the controller 12 is lower, a larger selection space can be provided during model selection of the controller 12, and the actual implementation is more convenient.

It is understood that, although not illustrated in the above embodiments, the electronic device such as the temperature detection apparatus must have a power supply or a power supply module for supplying power to each module, and similarly, the temperature detection apparatus provided by the present application also has a power supply module for supplying power to other module devices.

Illustratively, as shown in the above embodiment, the main control chip is STM32F407ZETG, the memory chip is a W25Q128FV chip, and the communication chip is an SP3232EEN chip, these chips and their working circuits used in cooperation mostly work at a supply voltage of 3.3V, and in practical applications, the DC power supply mostly supplies 24V, so the power supply module should have a DC-to-DC (DC-DC) converter for converting an externally input 24V power supply into a supply voltage of 3.3V for facilitating the operation of each chip of the temperature detection device. For the external 24V power input, it may be implemented by a foreign constant voltage power supply device, or by a portable power supply, and the like, which is not limited in this embodiment.

In addition, the present embodiment also provides a possible implementation manner of a DC-DC converter, which is a DC-DC converter with a model number U23XH2596, and the related circuits are shown in fig. 4, and include a DC-DC converter U23; a schottky diode D12; filter capacitances EC2, EC6.

In addition, this example also provides a preferred embodiment: an anti-reverse connection circuit is also arranged between the power supply and the DC-DC converter.

NMOS: the English is called N-Metal-Oxide-Semiconductor. To mean N-type metal-oxide-semiconductor, a transistor having such a structure is referred to as an NMOS transistor.

The reverse connection preventing circuit is used for protecting the temperature detecting device, avoiding risks such as circuit burnout caused by reverse connection of a power supply, and specifically, the reverse connection preventing circuit is as shown in fig. 5 and comprises: the power supply circuit comprises an NMOS tube Q1, a parasitic diode D1, a first voltage-dividing resistor R1, a second voltage-dividing resistor R1 and a first capacitor C1;

the grid electrode of the NMOS tube Q1 is connected with the negative electrode of a power supply, the source electrode of the NMOS tube Q1 is connected with the positive electrode of the power supply through a first divider resistor R1, and the drain electrode of the NMOS tube Q1 is connected with the negative electrode output end of the DC-DC converter;

a parasitic diode D1, a second voltage-dividing resistor R2 and a first capacitor C1 are connected in parallel with the grid and the source of the NMOS tube Q1; the anode of the parasitic diode D1 is correspondingly connected to the source of the NMOS transistor Q1.

Under the structure of the reverse connection preventing circuit of the embodiment, based on the principle that the on condition of the NMOS transistor Q1 is on when the voltage VGS between the gate and the source is >0, and otherwise, the NMOS transistor Q1 is turned off, the reverse connection of the power supply is prevented by using the NMOS transistor Q1. The NMOS tube Q1 is connected with the low side, namely the side close to the negative pole of the power supply; when the power supply is connected correctly, assuming that the power supply voltage is U, and the grid G of the NMOS tube Q1 is a high level U; due to the existence of the parasitic diode D1, the potential of the source electrode S of the NMOS tube Q1 is 0.7V, VGS is greater than 0, the NMOS tube Q1 is conducted, so that the load is electrified, and the circuit works normally; when the power supply is reversely connected, the gate G is at a low level, VGS =0, so the NMOS transistor Q1 is not turned on, and the circuit does not operate.

That is, when the polarity of the power supply is correct, the reverse-connection preventing circuit is conducted through the NMOS tube Q1 when the current starts, the voltage of the source electrode S is close to 0V, and after the voltage is divided by the two divider resistors, the voltage is provided for the grid electrode G, so that the NMOS tube Q1 is conducted, and the diode in the NMOS tube Q1 is replaced because the conducting resistance value is very small; when the power supply is reversely connected, the diode in the NMOS tube Q1 is not conducted when not reaching the breakdown voltage, and the divider resistor cannot provide the grid G voltage without current flowing through and is also not conducted, so that the protection effect is achieved.

Further, for the voltage conversion of the power module, the filtering circuit composed of the filter capacitor may also be used to perform filtering, so as to provide a more stable power voltage for the whole temperature detection apparatus, and as shown in fig. 6, a filtering circuit for the power module includes: a light emitting diode LED6; a filter capacitor EC1; a resistor R246; schottky diode D11.

The present embodiment further describes how the above-mentioned temperature detection device can supply power, and the DC-DC converter can convert the voltage input by the external power supply into an operating voltage suitable for the internal devices of the temperature detection device, thereby enhancing the compatibility of the device. In addition, an anti-reverse-connection circuit is added, by utilizing the switching characteristic of the NMOS tube, when the power supply is reversely connected, the anti-reverse-connection circuit is not conducted, the rear end load is not electrified, circuit protection is realized, when the power supply is correctly connected, the anti-reverse-connection circuit is conducted, the normal work of the temperature detection device is not influenced, and the safety of the temperature detection device is further improved.

Further, after the types of the thermistor sensor 13, the platinum thermistor sensor 14, and the thermocouple sensor 15 are determined, the temperature acquisition circuit 11 is a temperature acquisition circuit 11 used with the temperature sensor, such as an instrumentation amplifier circuit and some filter circuits, for performing a certain processing on the signals obtained by temperature acquisition to obtain more accurate and stable temperature information.

For the thermistor sensor 13 (specifically, an NTC thermistor), the present embodiment provides a preferable solution, and the temperature acquisition circuit 11 includes:

a filter circuit provided between the thermistor sensor 13 and the controller 12.

The filter circuit may be further divided into a capacitor filter of the front-end filter circuit and an RC filter circuit to filter interference in the signal collected by the thermistor sensor 13.

Further, this embodiment also provides a preferable scheme, and the temperature acquisition circuit 11 further includes:

and a voltage stabilizing circuit provided between the thermistor sensor 13 and the controller 12.

It can be known from the foregoing embodiments that the voltage regulator circuit in this embodiment should be a dc voltage regulator circuit, and the output voltage is kept stable by the regulating tube.

In addition, for the platinum thermistor sensor 14 (specifically, PT1000 platinum thermistor), this embodiment provides a preferable solution, and the temperature acquisition circuit 11 includes:

a differential circuit disposed between the platinum thermistor sensor 14 and the controller 12.

That is, the platinum thermistor is connected to the controller 12 in a differential manner. The platinum thermal resistor adopts a differential voltage input mode, so that the temperature drift can be effectively inhibited, and the temperature detection precision is improved.

For the thermocouple sensor 15 (which may be a type K thermocouple, in particular), the present embodiment provides a preferable solution, and the temperature acquisition circuit 11 includes:

a transient suppression circuit disposed between the thermocouple sensor 15 and the controller 12.

Transient suppression circuits are arranged on the thermocouple sensor 15 and the controller 12 and are used for suppressing surge current, so that the temperature acquisition channel can be protected, and reliable operation can still be guaranteed under extreme environments.

While the above provides a temperature detection device, this embodiment also provides a corresponding embodiment of a nucleic acid purification and amplification system, which includes a nucleic acid purification and amplification instrument and the above temperature detection device;

specifically, the nucleic acid purification and amplification system comprises: a 24V direct current power supply, a temperature acquisition board (namely a temperature acquisition circuit integrated on a circuit board), 12K-type thermocouples, 2 NTCs, 2 PTs 1000, a plurality of nucleic acid purification and amplification instruments to be tested, and 1 notebook computer (used as a controller of the temperature detection device or an upper computer communicated with the temperature detection device).

In this embodiment, the nucleic acid purification and amplification instrument is a full-automatic nucleic acid detection and analysis device, and is mainly divided into two modules, namely a nucleic acid purification module and a nucleic acid amplification module, wherein the nucleic acid purification module is firstly purified and then amplified, the two modules are different in mechanical design, the difference of application scenes during temperature verification is large, the nucleic acid purification module adopts surface contact type heating, and the temperature of a reagent needs to be controlled in a tiny reagent tube during nucleic acid amplification, so that the temperature sensor of the traditional single sensor cannot well meet the temperature detection requirement of the nucleic acid purification and amplification instrument.

Therefore, the temperature detection device is applied to two modules simultaneously to realize that: monitoring the temperature of a heating aluminum plate for nucleic acid purification during the nucleic acid purification process; then, the temperature control point and the temperature of the amplification plate are monitored during the nucleic acid amplification process.

The nucleic acid purification module adopts a resistance wire or other modes to heat an aluminum plate (the design thinking of the nucleic acid purification amplification instrument which is actually implemented may be slightly different), the aluminum plate transmits heat to the kit, and the nucleic acid is purified in the kit. The reagent kit has 36 columns in total, wherein 6 columns need to be heated, the heating control mode is current control, the temperature control requirement deviation of the aluminum plate is less than +/-0.5 ℃, and the temperature range is 30-85 ℃. In order to determine the heating current and verify the heating uniformity of the nucleic acid purification module, two temperature measuring points are selected from each column of 6 purified columns for temperature measurement, and the heating temperature is set to be 80 ℃. The requirement on the detection precision in the process is not high, so that the K-type thermocouple can be used for measurement.

The deviation of the control of the temperature of the nucleic acid amplification module is required to be less than +/-0.1 ℃, the heating mode of the module is a Peltier heating aluminum amplification plate, a temperature feedback sensor carried by the amplification module is attached above the Peltier, and the temperature control is carried out by adopting a PID (proportional, integral, differential control, PID control) temperature regulation method. The temperature control range is 60-95 ℃, the rapid temperature cycle is carried out, the maximum temperature rise speed is more than 6 ℃/s, and the maximum temperature drop speed is more than 4 ℃/s. Therefore, if the result of the temperature feedback sensor carried by the amplification module is to be calibrated, a more sensitive sensor is needed, namely, the precision reaches 0.01 ℃, and the temperature response speed of the module has higher requirement, so that the PT1000 temperature sensor is adopted, and the probe of the sensor is placed beside the temperature feedback sensor carried by the amplification module to monitor whether the temperature of the sensor is accurate or not.

And fitting the NTC with a proper size according to actual conditions to test the temperature of the aluminum amplification plate directly contacted with the amplification reagent above the Peltier so as to realize more accurate control of the temperature of the amplification plate.

Furthermore, the flow steps of the auxiliary temperature measurement process of the nucleic acid purification and amplification instrument of the embodiment are as follows:

1. connecting the sensor of the temperature detection device to each temperature detection point of the nucleic acid purification and amplification instrument, and establishing the communication connection relationship between the nucleic acid purification and amplification instrument main body and the temperature detection device.

The method specifically comprises the following steps:

a. and connecting the K-type thermocouples to an aluminum plate of a purification module of the nucleic acid purification and amplification instrument in a manner of smearing heat-conducting silicone grease and locking the heat-conducting silicone grease by using screws, wherein 2K-type thermocouples are respectively placed at 6 rows of reagent positions needing to be heated.

b. The PT1000 temperature sensor is placed at the temperature acquisition point of the amplification module of the nucleic acid purification and amplification instrument, and heat-conducting silicone grease is smeared to be tightly adhered to the temperature acquisition point.

c. Connecting the NTC to an aluminum amplification plate of an amplification module of the nucleic acid purification and amplification instrument.

d. The communication interface is accessed into a temperature control integrated circuit of an amplification module of the nucleic acid purification and amplification instrument to realize the communication between the two.

2. A purification module of the nucleic acid purifier purifies a sample to be detected in a reagent tube, and in the process, an aluminum plate is required to heat the sample to be detected, and the sample is required to be heated from 30 ℃ to 80 ℃ within 60 seconds. Detect aluminum plate's temperature through K type thermocouple to the signal of telecommunication with reaction temperature feeds back to temperature acquisition module, and communication module is transmitted with the temperature data who collects to data storage module, and finally transmits the host computer and shows.

Figure 7 is when setting for target temperature 80 ℃, adopts the utility model discloses a K type thermocouple carries out the result that the temperature was verified, and the temperature of visible purification module promotes 80 ℃ about 60s consuming time from 30 ℃, and the temperature fluctuation curve is more regular, and K type thermocouple can monitor this module temperature change betterly, and the heating current of current adoption also meets the demands.

3. The initial temperature of the nucleic acid amplification module is set to 35 ℃ when the reagent in the reagent tube is transferred to the amplification module in the nucleic acid purification instrument, and the data of the temperature sensor carried by the amplification instrument and the data of the PT1000 sensor of the temperature detection device are collected.

Fig. 8 shows the reading of the PT1000 upper computer and the result of the self-temperature acquisition of the amplification module when 35 ℃ is set, wherein the upper computer is the reading of the PT1000 upper computer, and the amplification module is the data acquired by the self-temperature sensor of nucleic acid amplification. It can be seen from the figure that the temperature detection accuracy of the PT1000 is higher than the temperature acquisition accuracy of the amplification module, and the whole trend is consistent, so that the deviation between the PT1000 result and the sensor of the amplification module is calculated and then stored in the amplification module, and the deviation value calibration and correction are performed on the two results, so that the accuracy of the sensor of the amplification module is improved.

4. And (4) performing amplification, and collecting data of the self-contained temperature sensor of the amplification module and data of the NTC sensor on the aluminum amplification plate after calibration in the step (3) in the amplification process. And further calibrating the data detected by the NTC sensor with the self-carried sensor of the amplification module.

FIG. 9 shows the results of NTC host reading and amplification module temperature control unit reading when 55 ℃ amplification pre-heating is set.

It can be known from the above description that, in the existing nucleic acid purification and amplification instrument (or for the nucleic acid purification and amplification instrument with the older model), the accuracy of the temperature sensor is low or the stability and the accuracy of the detection temperature are reduced after long-term use.

The temperature detection device and the nucleic acid purification and amplification system provided by the present application are described in detail above. The embodiments are described in a progressive manner in the specification, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description. It should be noted that, for those skilled in the art, it is possible to make several improvements and modifications to the present application without departing from the principle of the present application, and such improvements and modifications also fall within the scope of the claims of the present application.

It is further noted that, in the present specification, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrases "comprising a," "8230," "8230," or "comprising" does not exclude the presence of additional like elements in a process, method, article, or apparatus that comprises the element.

Claims (14)

1. A temperature detection device, comprising:

the temperature control system comprises a temperature acquisition circuit (11), a controller (12), a thermistor sensor (13), a platinum thermistor sensor (14) and a thermocouple sensor (15);

the controller (12) is connected with the thermistor sensor (13), the platinum thermistor sensor (14) and the thermocouple sensor (15) through the temperature acquisition circuit (11).

2. The temperature sensing device of claim 1, further comprising a memory (16) having stored therein temperature calibration parameters, the memory (16) being coupled to the controller (12).

3. The temperature detection device according to claim 1, further comprising a communication module (17) connected to the controller (12), the communication module (17) being provided with a TTL interface and an RS-232 interface.

4. The temperature detection device according to claim 1, wherein the thermistor sensor (13), the platinum thermistor sensor (14) and the thermocouple sensor (15) are connected to the temperature acquisition circuit (11) via interfaces, and the temperature acquisition circuit (11) is further provided with an expansion interface.

5. The temperature detection device according to claim 1, wherein the thermistor sensor (13), the platinum thermistor sensor (14), and the thermocouple sensor (15) are plural.

6. The temperature detection device according to claim 5, wherein specifications of the thermistor sensor (13), the platinum thermistor sensor (14), or the thermocouple sensor (15) are different.

7. The temperature sensing device according to claim 1, further comprising a DC-DC converter disposed between an external power source and the controller (12).

8. The temperature sensing device according to claim 7, further comprising an anti-reverse connection circuit provided between a power supply and the DC-DC converter.

9. The temperature sensing device according to claim 8, wherein the reverse connection prevention circuit includes: the device comprises an NMOS tube, a parasitic diode, a first voltage-dividing resistor, a second voltage-dividing resistor and a first capacitor;

the grid electrode of the NMOS tube is connected with the negative electrode of the power supply, the source electrode of the NMOS tube is connected with the positive electrode of the power supply through the first divider resistor, and the drain electrode of the NMOS tube is connected with the negative electrode output end of the DC-DC converter;

the parasitic diode, the second voltage-dividing resistor and the first capacitor are connected in parallel with the grid and the source of the NMOS tube; and the anode of the parasitic diode is correspondingly connected to the source electrode of the NMOS tube.

10. The temperature detecting device according to claim 1, wherein a crystal oscillator circuit of the controller (12) is provided with a plurality of crystal oscillators with different frequencies, each of the crystal oscillators is connected to the crystal oscillator circuit through a switch, and a control terminal of the switch is connected to the controller (12).

11. The temperature detection device according to claim 1, wherein the temperature acquisition circuit (11) comprises:

a filter circuit disposed between the thermistor sensor (13) and the controller (12);

and/or:

a voltage stabilizing circuit arranged between the thermistor sensor (13) and the controller (12);

and/or:

a differential circuit disposed between the platinum thermistor sensor (14) and the controller (12);

and/or:

a transient suppression circuit disposed between the thermocouple sensor (15) and the controller (12).

12. A nucleic acid purification and amplification system comprising the temperature detection device according to any one of claims 1 to 11 and a nucleic acid purification and amplification apparatus, wherein the nucleic acid purification and amplification apparatus comprises: a nucleic acid amplification module and a nucleic acid purification module.

13. The system for purifying and amplifying nucleic acid according to claim 12, wherein the thermistor sensor of the temperature detecting device is an NTC thermistor sensor, the platinum thermistor sensor is a PT1000 sensor, and the thermocouple sensor is a K-type thermocouple sensor;

the NTC thermistor sensor is arranged at an amplification plate of the nucleic acid amplification module;

the PT1000 sensor is arranged at a Peltier of the nucleic acid amplification module;

the type K thermocouple sensor is disposed at an amplification plate of the nucleic acid amplification module.

14. The system of claim 12, wherein the thermistor sensors of the temperature detecting device are NTC thermistor sensors, and at least two of them are respectively disposed at the amplification plate of the nucleic acid amplification module and the upper surface of the peltier of the nucleic acid amplification module.

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