CN218620853U

CN218620853U - Cell separation device

Info

Publication number: CN218620853U
Application number: CN202222725292.8U
Authority: CN
Inventors: 易翠林; 程金秋
Original assignee: Shanghai Hoeher Co ltd
Current assignee: Shanghai Hoeher Co ltd
Priority date: 2022-10-17
Filing date: 2022-10-17
Publication date: 2023-03-14
Anticipated expiration: 2032-10-17

Abstract

The utility model discloses a cell separation device, its technical scheme main points are: the separation tank comprises a separation tank, wherein a placing box is arranged on the bottom surface of the separation tank, and a fixing ring is fixedly connected inside the placing box; the filter screen is fixedly connected inside the fixing ring, and a rotating hole is formed in the top surface of the separation tank; coupling assembling, coupling assembling sets up the bottom surface of knockout drum, be used for with the knockout drum with place the box and connect, after the staff will rub tissue stoste, water and reaction enzyme send into the inside of knockout drum through a plurality of feed port respectively, drive the stirring leaf through the dwang when driving motor's drive shaft and rotate, when stirring leaf reciprocating rotation stirring, can be with mixed solution spiral-wound from top to bottom, carry out the fully reacted back, open the solenoid valve, make the inside solution of knockout drum filter through the filter screen, the stem cell after the separation is flowing through the valve and is collected, thereby improve mixed solution's reaction efficiency and improve stem cell's separation efficiency.

Description

Cell separation device

Technical Field

The utility model relates to a cell extraction field, concretely relates to cell separation device.

Background

Stem cells are a type of pluripotent cells that are capable of self-replication; under certain conditions, it can differentiate into multiple functional cells; dividing the stem cells into embryonic stem cells and adult stem cells according to the development stage of the stem cells; the stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent (multipotent); stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field.

For example, chinese patent publication No. CN210367673U proposes a stem cell separation device, in which a tissue stock solution is injected into a separation tank through a first feed pipe, a reciprocating drive mechanism operates to drive a rotation shaft to rotate, the rotation shaft drives a rolling cutter to rotate to rub the injected tissue stock solution, the solution enters a mixing cylinder through a first liquid outlet pipe, water and a reaction enzyme are injected into the mixing cylinder through a second feed pipe and a third feed pipe, at this time, the water, the reaction enzyme and the tissue solution flow down from a stirring blade below, the rotation shaft drives a stirring blade of a spiral structure to rotate in a reciprocating manner, the mixed solution can be rotated up and down, after sufficient reaction, a blocking component is opened to filter the solution, the separated stem cells are discharged and collected from a second liquid outlet, the reaction efficiency of the mixed solution is improved, thereby improving the separation efficiency of the stem cells, but after the cell separation device is used, the filter plate is not conveniently cleaned, so that bacteria are easily accumulated, thereby affecting the separation of the stem cells.

SUMMERY OF THE UTILITY MODEL

To solve the above problems, it is an object of the present invention to provide a cell separation device to solve the above problems.

The above technical purpose of the present invention can be achieved by the following technical solutions:

a cell separation device, comprising: the separation tank is provided with a placing box on the bottom surface, and a fixing ring is fixedly connected inside the placing box; the filter screen is fixedly connected inside the fixing ring, and the top surface of the separation tank is provided with a rotating hole; the connecting assembly is arranged on the bottom surface of the separating tank and used for connecting the separating tank with the placing box.

Through adopting above-mentioned technical scheme, through setting up coupling assembling, coupling assembling can fix the bottom surface at the knockout drum with placing the box, simultaneously, the staff later stage of being convenient for with the knockout drum with place the box separation.

Preferably, the connection assembly comprises: the connecting groove is formed in the bottom surface of the separating tank, the top surface of the placing box is fixedly connected with a connecting ring, and the connecting ring and the connecting groove are in threaded connection; the bottom surface of placing box inside has seted up the liquid outlet.

By adopting the technical scheme, through the arrangement of the separation tank, after the minced tissue stock solution, water and the reaction enzyme are respectively fed into the separation tank through the plurality of feed holes by a worker.

Preferably, the inside rotation in rotation hole installs the dwang, the excircle wall of dwang rotates installs the stirring leaf.

Through adopting above-mentioned technical scheme, through setting up the dwang, can drive the stirring leaf when the dwang rotates and rotate.

Preferably, the top end of the separation tank is fixedly connected with a driving motor, and the bottom end of the driving shaft of the driving motor is fixedly connected with the top end of the rotating rod.

Through adopting above-mentioned technical scheme, through setting up driving motor, drive the stirring leaf through the dwang and rotate as driving motor's drive shaft, stir leaf reciprocating rotation stirring, can be with mixed solution spiral-wound from top to bottom, carry out the abundant reaction.

Preferably, the inside fixedly connected with flow valve of feed port, the inside fixedly connected with solenoid valve of discharge opening.

Through adopting above-mentioned technical scheme, through opening the solenoid valve, make the inside solution of knockout drum filter through the filter screen, the stem cell after the separation is collecting through the valve outflow, thereby improves the reaction efficiency of mixed solution and improves the separation efficiency of stem cell, places the box through rotating to the bottom surface that the box can be followed to the messenger is placed and is taken off, thereby the staff's later stage of being convenient for is cleared up the filter screen etc..

Preferably, the inside fixedly connected with valve of liquid outlet, the bottom surface fixedly connected with a plurality of supporting leg of knockout drum.

Through adopting above-mentioned technical scheme, through setting up the supporting leg, the supporting leg can support the knockout drum.

To sum up, the utility model discloses mainly have following beneficial effect:

through setting up the knockout drum, tissue stoste after the staff will rub, water and reaction enzyme are sent into the inside back of knockout drum through a plurality of feed port respectively, through setting up driving motor, the drive shaft when driving motor passes through the dwang and drives the stirring leaf and rotate, when stirring leaf reciprocating rotation stirring, can be with mixed solution spiral-wound from top to bottom, carry out the abundant reaction back, through opening the solenoid valve, make the inside solution of knockout drum filter through the filter screen, the stem cell after the separation is flowing through the valve and is collecting, thereby improve the reaction efficiency of mixed solution and improve the separation efficiency of stem cell, and simultaneously, place the box through rotating, thereby make the bottom surface that places the box and can follow the knockout drum take off, thereby be convenient for the staff later stage to clear up the filter screen etc..

Drawings

Fig. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of the storage case of the present invention;

FIG. 3 is a schematic structural view of the separation tank of the present invention;

fig. 4 is a partially enlarged structural diagram of a in fig. 3.

Reference numerals: 1. a separation tank; 2. placing the box; 3. a fixing ring; 4. filtering with a screen; 5. rotating the hole; 6. connecting grooves; 7. a connecting ring; 8. a feed port; 9. a discharge hole; 10. a liquid outlet; 11. rotating the rod; 12. stirring blades; 13. a drive motor; 14. a flow valve; 15. an electromagnetic valve; 16. a valve; 17. and (5) supporting legs.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.

Refer to fig. 1, fig. 2 and fig. 3, a cell separation device, including knockout drum 1, box 2 is placed in the bottom surface setting of knockout drum 1, the solid fixed ring 3 of inside fixedly connected with of placing box 2, the inside fixedly connected with filter screen 4 of solid fixed ring 3, rotation hole 5 has been seted up to the top surface of knockout drum 1, the bottom surface of knockout drum 1 is provided with coupling assembling, be used for being connected knockout drum 1 with placing box 2, coupling assembling includes spread groove 6, spread groove 6 sets up the bottom surface at knockout drum 1, screw has been seted up to the interior round wall of spread groove 6, place the top surface fixedly connected with go-between 7 of box 2, screw has been seted up to the exterior round wall of go-between 7, go-between 7 and spread groove 6 threaded connection together, a plurality of feed port 8 has been seted up to the top surface of knockout drum 1, discharge opening 9 has been seted up to the bottom surface of knockout drum 1 inside, place liquid outlet 10 of box 2 inside, through setting up knockout drum 1, when the tissue stoste after the staff will rub, water and reaction enzyme carry out the inside of knockout drum 1 through a plurality of feed port 8 respectively, thereby be convenient for mixed solution mixed reaction, the later stage is got the later stage from the staff and the filter screen 4, thereby make the later stage of placing box 2 take out.

Referring to fig. 2, fig. 3 and fig. 4, the inside of rotation hole 5 is rotated and is installed dwang 11, the excircle wall of dwang 11 is rotated and is installed stirring leaf 12, the top fixedly connected with driving motor 13 of knockout drum 1, the bottom of driving motor 13 drive shaft is in the same place with the top fixed connection of dwang 11, the inside fixedly connected with flow valve 14 of feed port 8, through setting up flow valve 14, flow valve 14 can control the tissue stoste that gets into the knockout drum 1 inside, water and reaction enzyme, the inside fixedly connected with solenoid valve 15 of discharge opening 9, the inside fixedly connected with valve 16 of liquid outlet 10, through setting up driving motor 13, drive shaft through dwang 11 drive stirring leaf 12 when driving motor 13 rotates, when stirring leaf reciprocating rotation stirring, can be with mixed solution up and down, after fully reacting, through opening solenoid valve 15, make the inside solution of knockout drum 1 filter through filter screen 4, the stem cell after the separation is flowing out through valve 16 and is collected, improve the reaction efficiency of mixed solution and thereby improve the separation efficiency of stem cell, the bottom surface fixed connection of knockout drum 1 has a plurality of supporting legs 17.

The working principle is as follows: referring to fig. 1-4, in use, by arranging the separation tank 1, when a worker sends minced tissue stock solution, water and reactive enzyme into the separation tank 1 through a plurality of feed holes 8, respectively, by arranging the driving motor 13, when the driving shaft of the driving motor 13 drives the stirring blade 12 to rotate through the rotating rod 11, when the stirring blade rotates and stirs in a reciprocating manner, the mixed solution can be screwed up and down, after full reaction, the solution in the separation tank 1 is filtered through the filter screen 4 by opening the electromagnetic valve 15, and the separated stem cells flow out and are collected through the valve 16, so that the reaction efficiency of the mixed solution is improved, and the separation efficiency of the stem cells is improved, and meanwhile, by rotating the placing box 2, the placing box 2 can be taken down from the bottom surface of the separation tank 1, so that the worker can clean the filter screen 4 in the later period, and the like.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell separation device, comprising:

the separation tank (1), wherein a placing box (2) is arranged on the bottom surface of the separation tank (1), and a fixing ring (3) is fixedly connected inside the placing box (2);

the filter screen (4) is fixedly connected inside the fixing ring (3), and the top surface of the separation tank (1) is provided with a rotating hole (5);

the connecting assembly is arranged on the bottom surface of the separating tank (1) and used for connecting the separating tank (1) with the placing box (2).

2. A cell separation apparatus according to claim 1, wherein the connecting assembly comprises:

the connecting groove (6) is formed in the bottom surface of the separating tank (1), a connecting ring (7) is fixedly connected to the top surface of the placing box (2), and the connecting ring (7) is in threaded connection with the connecting groove (6);

a plurality of feed port (8), a plurality of feed port (8) are all seted up the top surface of knockout drum (1), discharge opening (9) have been seted up to the inside bottom surface of knockout drum (1), place the inside bottom surface of box (2) and seted up liquid outlet (10).

3. A cell separation device according to claim 2, wherein the inside of the rotating hole (5) is rotatably provided with a rotating rod (11), and the outer circular wall surface of the rotating rod (11) is rotatably provided with a stirring blade (12).

4. A cell separation device according to claim 3, wherein a driving motor (13) is fixedly connected to the top end of the separation tank (1), and the bottom end of the driving shaft of the driving motor (13) is fixedly connected with the top end of the rotating rod (11).

5. A cell separation device according to claim 2, wherein a flow valve (14) is fixedly connected to the inside of the inlet port (8), and a solenoid valve (15) is fixedly connected to the inside of the outlet port (9).

6. A cell separation apparatus according to claim 2, wherein a valve (16) is fixedly connected to the interior of the liquid outlet (10), and a plurality of support legs (17) are fixedly connected to the bottom surface of the separation tank (1).