CN210367673U - Stem cell separation device - Google Patents
Stem cell separation device Download PDFInfo
- Publication number
- CN210367673U CN210367673U CN201921055342.8U CN201921055342U CN210367673U CN 210367673 U CN210367673 U CN 210367673U CN 201921055342 U CN201921055342 U CN 201921055342U CN 210367673 U CN210367673 U CN 210367673U
- Authority
- CN
- China
- Prior art keywords
- wall
- liquid outlet
- feeding pipe
- stem cell
- baffle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a stem cell separation device, belonging to the technical field of cell extraction; a stem cell separation device comprises a separation tank, wherein the top of a second baffle is connected with a mixing cylinder, a reciprocating driving mechanism is connected inside a shell, a rod wall of a rotating shaft, which is arranged between the inner wall of the top of the separation tank and the top wall of a first baffle, is connected with a reel knife, a rod wall of the rotating shaft, which is arranged inside the mixing cylinder, is connected with a stirring blade, a second feeding pipe, a third feeding pipe and one end of a first liquid outlet pipe, which is arranged inside the mixing cylinder, are arranged on the inner wall of the top of the mixing cylinder at equal intervals, the bottom of the second baffle is connected with a blocking assembly, and the side wall, which is close to; the utility model discloses a rolling cutter rotates and rubs the tissue stoste and carries out the preliminary treatment, later through second inlet pipe, third inlet pipe and first drain pipe respectively with water, reaction enzyme and tissue stoste injection mixing drum in by stirring leaf stirring mixing reaction, thereby make the reaction improve stem cell's separation efficiency more thoroughly.
Description
Technical Field
The utility model relates to a cell extraction technical field especially relates to a stem cell separator.
Background
Stem cells are a type of pluripotent cells with the ability to self-replicate; under certain conditions, it can differentiate into multiple functional cells; dividing the stem cells into embryonic stem cells and adult stem cells according to the development stage of the stem cells; the stem cells are classified into three categories according to their developmental potential: totipotent, pluripotent and unipotent stem cells (multipotent stem cells); stem cells are insufficiently differentiated and immature cells, have potential functions of regenerating various tissues, organs and human bodies, and are called universal cells in the medical field.
The stem cells are often mixed with other cells after extraction, so a stem cell separation device is needed; the conventional method for obtaining the stem cell population comprises the steps of manually shearing adipose tissues and then filtering, wherein the obtained number of stem cells is in direct proportion to the shearing degree of the adipose tissues, the separation step comprises the steps of injecting stem cell stock solution, water and enzyme into a reaction container for reaction, and collecting extract after the reaction is completed to complete the separation step.
Most of the existing stem cell separation devices directly place stem cell tissue fluid, water and reactive enzyme into a device for reaction, and adipose tissue fragments cut into pieces manually are different in size, so that the reaction is not thorough, the extraction efficiency is poor, and a stem cell separation device is provided for solving the problem.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the problems in the prior art and providing a stem cell separation device.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a stem cell separation device comprises a separation tank, wherein a first baffle, a second baffle and a filter plate are sequentially connected to the inner side wall of the separation tank in a vertical direction, the top of the second baffle is connected with a mixing barrel, the top of the separation tank is connected with a shell, a reciprocating driving mechanism is connected to the interior of the shell, a rotating shaft is rotatably connected to the inner wall of the top of the shell, one end, away from the inner wall of the top of the shell, of the rotating shaft extends downwards to the interior of the mixing barrel, a winding knife is connected to a rod wall, arranged between the inner wall of the top of the separation tank and the top wall of the first baffle, of the rotating shaft, a stirring blade is connected to the rod wall, arranged inside the mixing barrel, of the rotating shaft, the top of the separation tank is connected with a first inlet pipe, a second inlet pipe and a third inlet pipe are connected to the side wall of the separation tank, open at first baffle top has first liquid outlet, first liquid outlet is connected with first drain pipe, the one end slope that first liquid outlet was kept away from to first drain pipe sets up inside the mixing drum, the one end equidistance that the mixing drum inside was arranged in to second inlet pipe, third inlet pipe and first drain pipe sets up on mixing drum top inner wall, the mixing drum bottom is opened with second baffle top has the second liquid outlet, second baffle bottom is connected with and blocks up the subassembly, be connected with the second drain pipe on the lateral wall that the knockout drum is close to the bottom.
Preferably, the reciprocating driving mechanism comprises a driving half-gear and a complete gear, the driving half-gear is connected to the inner wall of the top of the shell, the complete gear is connected to the rod wall of the rotating shaft arranged inside the shell, the driving half-gear is meshed with the complete gear, and a spring sleeved on the rod wall of the rotating shaft is connected between the inner wall of the top of the shell and the complete gear.
Preferably, the blocking assembly comprises a blocking cover and a screw rod, the screw rod is in threaded connection with the side wall of the separation tank, the blocking cover is rotationally connected with the screwing-in end of the screw rod, and the blocking cover abuts against the bottom of the second blocking plate.
Preferably, the stirring blade has a spiral structure.
Preferably, the second feeding pipe, the third feeding pipe and the first liquid outlet pipe are positioned above the stirring blade.
Preferably, the outer walls of the first feeding pipe, the second feeding pipe, the third feeding pipe and the second liquid outlet pipe are all connected with flow valves.
Compared with the prior art, the utility model provides a stem cell separator possesses following beneficial effect:
1. this stem cell separator, it is inside with tissue stoste injection knockout drum to pour into the tissue stoste into through first inlet pipe, reciprocal actuating mechanism work drives the pivot and rotates, the pivot drives the rolling cutter and rotates the back that rubs the tissue stoste that will pour into, solution gets into the mixing drum through first drain pipe inside, inside rethread second inlet pipe and third inlet pipe pour into water and reaction enzyme into the mixing drum, water this moment, reaction enzyme and tissue solution flow down from the stirring leaf below together, the pivot drives helical structure's stirring leaf reciprocating rotation, can be with the mixed solution up-and-down spiral, carry out the abundant reaction after, open and block up the subassembly and make solution filtered by the filter plate, the stem cell after the separation is discharged from the second liquid outlet and is collected, thereby improve the reaction efficiency of mixed solution and improve the separation efficiency of stem cell.
2. This stem cell separator can drive complete gear rotation when the one side that drive half round of tooth gear has the tooth and complete gear contact, on drive half round of tooth gear does not have the one side of tooth and complete gear contact, can drive complete gear and reset under the effort of spring, thereby make the reciprocal rotation of complete gear drive pivot reciprocal rotation.
3. This stem cell separator, when tissue stoste, water and reaction enzyme mix the reaction in the mixing drum inside, drive through rotating the screw rod and move the fender lid that links to each other rather than rotating and block the second liquid outlet below the second liquid outlet, make solution mix reaction time extension, rotate the screw rod again and drive the fender lid and leave the second liquid outlet and make solution flow out after mixing reaction a period.
4. The stem cell separation device is convenient to control and add reaction liquid with proper proportion through a flow valve.
Drawings
Fig. 1 is a schematic structural diagram of a stem cell separation device according to the present invention;
fig. 2 is a schematic view of an internal structure of a stem cell separation device according to the present invention;
fig. 3 is a schematic view of an internal structure of a stem cell separation device according to the present invention.
In the figure: 1. a separation tank; 101. a first baffle plate; 102. a second baffle; 103. filtering the plate; 2. a housing; 3. a mixing drum; 4. a driving half-gear; 5. a complete gear; 6. a rotating shaft; 7. a spring; 8. rolling a cutter; 9. stirring blades; 10. a first liquid outlet pipe; 11. a second feed tube; 12. a third feed pipe; 13. a first feed tube; 14. a flow valve; 15. a blocking cover; 16. a screw; 17. a second liquid outlet pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Example 1:
referring to fig. 1-3, a stem cell separation device comprises a separation tank 1, a first baffle plate 101, a second baffle plate 102 and a filter plate 103 are sequentially connected on the inner side wall of the separation tank 1 along the vertical direction, a mixing cylinder 3 is connected on the top of the second baffle plate 102, a shell 2 is connected on the top of the separation tank 1, a reciprocating driving mechanism is connected inside the shell 2, a rotating shaft 6 is rotatably connected on the inner wall of the top of the shell 2, one end of the rotating shaft 6, which is far away from the inner wall of the top of the shell 2, extends downwards into the mixing cylinder 3, a winding knife 8 is connected on the rod wall of the rotating shaft 6, which is arranged between the inner wall of the top of the separation tank 1 and the top wall of the first baffle plate 101, a stirring blade 9 is connected on the rod wall of the rotating shaft 6, the top of the separation tank 1 is connected with a first feeding pipe 13, a second feeding pipe 11 and a third feeding pipe 12 are connected on the side wall of, a first liquid outlet is formed in the top of the first baffle plate 101, the first liquid outlet is connected with a first liquid outlet pipe 10, one end, far away from the first liquid outlet, of the first liquid outlet pipe 10 is obliquely arranged inside the mixing cylinder 3, a second feeding pipe 11, a third feeding pipe 12 and one end, placed inside the mixing cylinder 3, of the first liquid outlet pipe 10 are equidistantly arranged on the inner wall of the top of the mixing cylinder 3, a second liquid outlet is formed in the bottom of the mixing cylinder 3 and the top of the second baffle plate 102, the bottom of the second baffle plate 102 is connected with a blocking assembly, and a second liquid outlet pipe 17 is connected to the side wall, close to the bottom, of the;
the stirring blade 9 is of a spiral structure;
the second feeding pipe 11, the third feeding pipe 12 and the first liquid outlet pipe 10 are positioned above the stirring blade 9;
inject the tissue stoste into knockout drum 1 inside through first inlet pipe 13, reciprocal actuating mechanism work drives pivot 6 and rotates, pivot 6 drives the sword 8 and rotates the tissue stoste that will pour into after rubbing, solution gets into mixing drum 3 inside through first drain pipe 10, rethread second inlet pipe 11 and third inlet pipe 12 inject water and reaction enzyme into mixing drum 3 inside, water this moment, reaction enzyme and tissue solution flow down from the stirring leaf 9 of below together, pivot 6 drives the reciprocal rotation of stirring leaf 9 of helical structure, can be with mixing solution up-and-down spin, after carrying out the abundant reaction, open and block up the subassembly and make solution filtered by filter plate 103, the stem cell after the separation is followed second drain pipe 17 and is discharged the collection, thereby improve mixing solution's reaction efficiency and improve stem cell's separation efficiency.
Example 2:
referring to fig. 2-3, a stem cell separation device, substantially the same as in example 1, except that the reciprocating driving mechanism comprises a driving half-gear 4 and a complete gear 5, the driving half-gear 4 is connected to the inner wall of the top of the housing 2, the complete gear 5 is connected to a rod wall of the rotating shaft 6 arranged inside the housing 2, the driving half-gear 4 is engaged with the complete gear 5, and a spring 7 sleeved on the rod wall of the rotating shaft 6 is connected between the inner wall of the top of the housing 2 and the complete gear 5;
when the toothed surface of the driving half-gear toothed gear 4 is in contact with the complete gear 5, the complete gear 5 is driven to rotate, and when the non-toothed surface of the driving half-gear toothed gear 4 is in contact with the complete gear 5, the complete gear 5 is driven to reset under the action of the spring 7, so that the complete gear 5 rotates in a reciprocating manner to drive the rotating shaft 6 to rotate in a reciprocating manner.
Example 3:
referring to fig. 2-3, a stem cell separating device is substantially the same as that of example 2, except that the blocking assembly comprises a blocking cover 15 and a screw 16, the screw 16 is screwed on the side wall of the separating tank 1, the blocking cover 15 is rotatably connected with the screwing end of the screw 16, and the blocking cover 15 abuts against the bottom of the second baffle 102;
when the tissue stock solution, the water and the reaction enzyme are mixed and reacted in the mixing cylinder 3, the screw 16 is rotated to drive the blocking cover 15 which is rotationally connected with the screw to move to the lower part of the second liquid outlet so as to block the second liquid outlet, so that the solution mixing and reacting time is prolonged, and after the mixing and reacting are carried out for a period of time, the screw 16 is rotated to drive the blocking cover 15 to leave the second liquid outlet so as to enable the solution to flow out.
Example 4:
referring to fig. 1 to 3, a stem cell separation apparatus, which is substantially the same as that of embodiment 1, further, a flow valve 14 is connected to the outer walls of the first feeding pipe 13, the second feeding pipe 11, the third feeding pipe 12 and the second discharging pipe 17;
the controlled addition of the appropriate ratio of reaction solution is facilitated by flow valve 14.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The stem cell separation device comprises a separation tank (1) and is characterized in that a first baffle (101), a second baffle (102) and a filter plate (103) are sequentially connected to the inner side wall of the separation tank (1) in a vertical direction in an extending mode, the top of the second baffle (102) is connected with a mixing drum (3), the top of the separation tank (1) is connected with a shell (2), the inner side of the shell (2) is connected with a reciprocating driving mechanism, the inner wall of the top of the shell (2) is rotatably connected with a rotating shaft (6), one end, far away from the inner wall of the top of the shell (2), of the rotating shaft (6) extends downwards to the inside of the mixing drum (3), the rotating shaft (6) is connected with a rolling knife (8) on a rod wall between the inner wall of the top of the separation tank (1) and the top wall of the first baffle (101), and a stirring blade (9) is connected on the rod, the separating tank is characterized in that a first feeding pipe (13) is connected to the top of the separating tank (1), a second feeding pipe (11) and a third feeding pipe (12) are connected to the side wall of the separating tank (1), one end, far away from the side wall of the separating tank (1), of the second feeding pipe (11) and the third feeding pipe (12) is obliquely arranged inside the mixing cylinder (3), a first liquid outlet is formed in the top of the first baffle plate (101), the first liquid outlet is connected with a first liquid outlet pipe (10), one end, far away from the first liquid outlet, of the first liquid outlet is obliquely arranged inside the mixing cylinder (3) through the first liquid outlet pipe (10), one end, far away from the first liquid outlet, of the second feeding pipe (11), the third feeding pipe (12) and the first liquid outlet pipe (10) are equidistantly arranged on the inner wall of the top of the mixing cylinder (3), a second liquid outlet is formed in the bottom of the mixing cylinder (3) and the top, the bottom of the second baffle (102) is connected with a blocking assembly, and the side wall of the separation tank (1) close to the bottom is connected with a second liquid outlet pipe (17).
2. The stem cell separation device according to claim 1, wherein the reciprocating driving mechanism comprises a driving half-gear (4) and a complete gear (5), the driving half-gear (4) is connected to the inner wall of the top of the housing (2), the complete gear (5) is connected to the rod wall of the rotating shaft (6) arranged inside the housing (2), the driving half-gear (4) is meshed with the complete gear (5), and a spring (7) sleeved on the rod wall of the rotating shaft (6) is connected between the inner wall of the top of the housing (2) and the complete gear (5).
3. The stem cell separation device according to claim 2, wherein the blocking component comprises a blocking cover (15) and a screw (16), the screw (16) is screwed on the side wall of the separation tank (1), the blocking cover (15) is rotatably connected with the screwing end of the screw (16), and the blocking cover (15) abuts against the bottom of the second baffle (102).
4. A stem cell separation device according to any one of claims 1 to 3, wherein the stirring blade (9) has a helical structure.
5. A stem cell separation device according to any one of claims 1 to 3, wherein the second feeding pipe (11), the third feeding pipe (12) and the first liquid outlet pipe (10) are located above the stirring blade (9).
6. A stem cell separation device according to any one of claims 1 to 3, wherein the outer walls of the first feeding pipe (13), the second feeding pipe (11), the third feeding pipe (12) and the second outlet pipe (17) are connected with a flow valve (14).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921055342.8U CN210367673U (en) | 2019-07-08 | 2019-07-08 | Stem cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921055342.8U CN210367673U (en) | 2019-07-08 | 2019-07-08 | Stem cell separation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210367673U true CN210367673U (en) | 2020-04-21 |
Family
ID=70270792
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921055342.8U Active CN210367673U (en) | 2019-07-08 | 2019-07-08 | Stem cell separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210367673U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112029709A (en) * | 2020-09-11 | 2020-12-04 | 孙震颖 | Culture method for induced differentiation of stem cells |
| CN112129598A (en) * | 2020-09-02 | 2020-12-25 | 西安交通大学第二附属医院 | Tissue extraction device for breast tumor |
| CN112457977A (en) * | 2020-12-13 | 2021-03-09 | 万光超 | Rotary biological stem cell extractor and extraction method thereof |
| CN117797686A (en) * | 2024-03-01 | 2024-04-02 | 顶能科技有限公司 | Mixing device for integrated recycling of chive byproducts |
-
2019
- 2019-07-08 CN CN201921055342.8U patent/CN210367673U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112129598A (en) * | 2020-09-02 | 2020-12-25 | 西安交通大学第二附属医院 | Tissue extraction device for breast tumor |
| CN112029709A (en) * | 2020-09-11 | 2020-12-04 | 孙震颖 | Culture method for induced differentiation of stem cells |
| CN112457977A (en) * | 2020-12-13 | 2021-03-09 | 万光超 | Rotary biological stem cell extractor and extraction method thereof |
| CN112457977B (en) * | 2020-12-13 | 2021-10-08 | 陕西佰瑞衡健康科技有限公司 | Rotary biological stem cell extractor and extraction method thereof |
| CN117797686A (en) * | 2024-03-01 | 2024-04-02 | 顶能科技有限公司 | Mixing device for integrated recycling of chive byproducts |
| CN117797686B (en) * | 2024-03-01 | 2024-05-10 | 顶能科技有限公司 | Mixing device for integrated recycling of chive byproducts |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN210367673U (en) | Stem cell separation device | |
| CN207401478U (en) | A kind of new point of copper reaction kettle | |
| CN220364410U (en) | Wastewater treatment device | |
| CN112893387A (en) | Organic matter garbage decomposition device capable of completing decomposition treatment by utilizing self heat | |
| CN214781220U (en) | Sewage filtering, discharging and treating device | |
| CN211586611U (en) | Neutralization reaction kettle | |
| CN210711073U (en) | Water treatment device of furniture paint spraying and purifying equipment | |
| CN213427842U (en) | Scale cleaning device is bred to high definition water body high density | |
| CN209989364U (en) | Intelligent stem cell extraction and identification device | |
| CN208648980U (en) | A kind of sewage disposal system that effluent quality is good | |
| CN212102344U (en) | COD equipment is removed to stirring station sewage | |
| CN216073626U (en) | Biogas slurry fermentation device for biological organic fertilizer | |
| CN221287574U (en) | Food additive safety proportioning device | |
| CN220737921U (en) | Seaweed broken wall device | |
| CN218901626U (en) | Mixing arrangement is used in production of medical silicone scar gel | |
| CN219701915U (en) | Biological carbon source production neutralization device | |
| CN216878838U (en) | Essential oil production is with batching jar | |
| CN215963338U (en) | Fertile agitating unit of rich selenium | |
| CN219136708U (en) | Cosmetic essential oil extraction device | |
| CN221440461U (en) | Full-automatic water treatment processor | |
| CN220684806U (en) | Sewage treatment device convenient to clearance filter screen plate | |
| CN215783233U (en) | Balanced rod-type water treatment medicament ration adds device | |
| CN221333852U (en) | Auxiliary batching device | |
| CN218460423U (en) | Organic waste recycling device | |
| CN108245925A (en) | A kind of medicament active composition separator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |