CN218588996U - Optical fiber device for detecting and analyzing heart rate of zebra fish in real time - Google Patents
Optical fiber device for detecting and analyzing heart rate of zebra fish in real time Download PDFInfo
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- CN218588996U CN218588996U CN202122990787.9U CN202122990787U CN218588996U CN 218588996 U CN218588996 U CN 218588996U CN 202122990787 U CN202122990787 U CN 202122990787U CN 218588996 U CN218588996 U CN 218588996U
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Abstract
The utility model discloses an optical fiber device for detecting and analyzing the heart rate of zebra fish in real time; the device comprises a fixed groove, an optical signal transmitting end and an optical signal receiving and processing end; the fixing groove is used for placing and fixing zebra fish; the top end of the fixed groove is provided with a detection signal input port, and the bottom end of the fixed groove is provided with a detection signal output port corresponding to the detection signal input port; the optical signal transmitting end is connected with the detection signal input port; the optical signal receiving and processing end is connected with the detection signal output port. The heart of the zebra fish is irradiated by an optical fiber lead-in light source, the irradiated light signal is received by a spectrometer, and then the signal output by the spectrometer is subjected to algorithm calculation to obtain the heart rate of the zebra fish. The utility model discloses utilize the light signal to detect, compare with usual microscopy, greatly reduced the error that manual operation brought, can show reinforcing rhythm of the heart measuring accuracy.
Description
Technical Field
The utility model relates to an optical fiber sensing technical field, more specifically the optical fiber device who relates to a real-time detection and analysis zebra fish rhythm of heart that says so.
Background
The zebra fish is a common tropical fish, the gene of the zebra fish has 87% high similarity with the human gene, and the experimental result obtained by the drug experiment on the zebra fish is also suitable for the human body in most cases. Moreover, the zebra fish has strong reproductive capacity, short reproduction period and large egg laying amount, and the fertilized eggs can hatch the fry after 2 to 3 days. In addition, the zebra fish is small and exquisite in individual, convenient to culture, transparent in embryo body and easy to observe the influence of the medicine on the internal organs of the zebra fish. Therefore, the zebra fish can be used as a model of human diseases and used for judging the action and effect of the medicament on human treatment.
Heart disease is a complex disease, and the heart rate is the most intuitive reflection of whether the heart functions normally or not. For people at high risk of cardiovascular disease, long-term continuous monitoring of heart rate can establish a first line of defense against heart attack. By continuously and accurately monitoring and analyzing the heart rate in real time, abnormal heart activity can be found in time, and precious diagnosis and intervention time is won for patients. Due to the high similarity of zebra fish and human genes, monitoring the heart rate variation of zebra fish can provide reference for health monitoring of human heart. Through the real-time measurement of the zebra fish heart rate, very useful health information can be accurately calculated, the human heart disease can be prejudged, and the human heart disease can be treated and prevented in a targeted manner as soon as possible, so that the real-time measurement of the zebra fish heart rate has important significance for researching the prevention and treatment of the human heart disease.
At present, many researchers have carried out the research on the heart rate of the zebra fish, for example, the research on the embryonic development and the heart toxicity of the zebra fish by arecoline hydrobromide of the consortium of jiaji and dong haipeng is published in the global traditional Chinese medicine 2021.14 (07), the research on the toxic action of bisphenol AP on the heart development of the zebra fish is published in the environmental science and technology 2019.42 (08), the influence on the embryonic development, the angiogenesis and the heart by isoliquiritigenin of the zebra fish is published in the niche animal 2018.37 (06), the influence on the embryonic development, the angiogenesis and the heart by isoliquiritigenin of the consortium of the zebra fish is published in the thinking, the influence on the embryo development, the angiogenesis and the heart of the zebra fish is published in the Chinese medical journal 1.39 (01), the method for monitoring the heart rate of the zebra fish by different geng hein shunner tablets 203miso extract and the heart rhythm of the consortium is published in the university of the hamstrings 20252, the hamel industrial journal 2020, the cocklebur industry 2020, the university and the yellow croaker detection method for identifying the zebra fish. In the process of monitoring the heart rate of the zebra fish, the researchers adopt a microscopic observation method. However, since the heart diameter of zebra fish is generally about 100 μm, the method of microscopic observation has certain difficulties in operation and observation, and is easily affected by adverse factors such as artificial operation and environmental noise.
Therefore, how to provide a simple and effective optical fiber device for detecting and analyzing the heart rate of zebra fish in real time is a problem to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides an optical fiber device of real-time detection and analysis zebra fish rhythm of heart aims at solving above-mentioned technical scheme.
In order to realize the purpose, the utility model adopts the following technical scheme:
an optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, comprising:
fixing grooves; the fixing groove is used for placing and fixing zebra fish; the top end of the fixed groove is provided with a detection signal input port, and the bottom end of the fixed groove is provided with a detection signal output port corresponding to the detection signal input port;
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port;
an optical signal receiving and processing terminal; and the optical signal receiving and processing end is connected with the detection signal output port and is used for carrying out algorithm processing on the received optical signals so as to obtain the heart rate of the zebra fish.
Through the technical scheme, the utility model provides an optical fiber device adopts the fixed mode with the zebra fish, utilizes light signal transmission end and light signal reception processing terminal pair zebra fish to carry out optical signal processing, can guarantee that the zebra fish is not receiving the heart rate change of real-time supervision zebra fish under the condition of damage.
Preferably, in the optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the fixing groove is wrapped with a metal shell. By using the metal closed box, the interference of adverse factors such as environmental noise and the like is effectively avoided or weakened; the fixing groove is a cuboid fixing groove with the volume slightly larger than that of the zebra fish.
Preferably, in the above optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the optical signal transmitting end includes a light source, and a first optical fiber bundle connected to the transmitting end of the light source; and one end of the first optical fiber bundle, which is far away from the light source, is connected with the detection signal input port. The light signal output of the light source can be satisfied.
Preferably, in the above-mentioned optical fiber device for real-time detection and analysis of zebra fish heart rate, the optical signal receiving and processing end includes with the second fiber bundle that the signal output port is connected, and with the second fiber bundle is kept away from the spectrum appearance that signal output port one end is connected, and with the output port of spectrum appearance electric connection's in proper order signal acquisition module, AD conversion module, singlechip and liquid crystal display. The singlechip carries out algorithm processing on the fed-back signals and transmits the processed signals to the liquid crystal display screen to display a heart rate graph of the zebra fish.
Preferably, in the above optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the optical signal receiving and processing end further includes a temperature sensor installed inside the fixing groove; the temperature sensor is electrically connected with the signal acquisition module through a signal wire and can detect the temperature in the fixed groove.
Preferably, in the above optical fiber apparatus for detecting and analyzing the heart rate of the zebra fish in real time, the heart of the zebra fish fixed in the fixing groove is located within the light spot range introduced by the detection signal input port, and the eyes of the zebra fish are located outside the light spot range introduced by the detection signal input port. Can detect the zebra fish rhythm of the heart, and prevent to cause the damage to its eyes.
The utility model also provides a method of real-time detection and analysis zebra fish rhythm of the heart shines fixed zebra fish heart position through optic fibre leading-in light source to optical signal after shining receives through the spectrum appearance, then carries out the algorithm calculation to the signal of spectrum appearance output, and then reachs the rhythm of the heart of zebra fish.
Through the technical scheme, the utility model provides a method need not to implant the electrode, utilizes the light signal to detect, compares with usual microscopy, greatly reduced the error that manual operation brought, can show reinforcing rhythm of the heart measuring accuracy.
The algorithm adopts a frame brightness algorithm or a variable pixel intensity algorithm. Tracking the change of the average light intensity of each frame of image by adopting a frame brightness algorithm, and calculating the heart rate; or a variable pixel intensity algorithm to detect heart rate by comparing the intensity variation of pixels from one frame to the next.
The zebra fish is non-labeled zebra fish or zebra fish labeled by a luminescent group or a fluorescent group; the spectrum signal tested by the spectrometer comprises a signal caused by the heart beat of non-labeled zebra fish or a luminescent signal or a fluorescent signal caused by the heart beat of zebra fish marked by a luminescent group or a fluorescent group. The detection applicability is stronger.
The zebra fish used for detection is in a fixed state or a sleeping state, so that the influence of factors such as swimming, water flow and the like of the zebra fish is avoided, and the actual heart rate of the zebra fish can be more accurately measured.
Can know via foretell technical scheme, compare with prior art, the utility model discloses an optical fiber device of real-time detection and analysis zebra fish rhythm of heart has following beneficial effect:
1. the utility model discloses need not to implant the electrode, can guarantee that the zebra fish is not receiving the rhythm of the heart change of real-time supervision zebra fish under the condition of damage.
2. The utility model discloses an use metal enclosed box, avoided or weakened the interference of unfavorable factors such as ambient noise effectively.
3. The utility model discloses a with the fixed mode of zebra fish, avoided the zebra fish to move about, the influence of factors such as rivers, can determine the actual rhythm of the heart of zebra fish more accurately.
4. The utility model discloses compare with usual microscopy, greatly reduced the error that manual operation brought, can show reinforcing rhythm of the heart measuring accuracy.
5. The utility model discloses a study external environment's change and provide certain reference to the influence of human heart and the judgement of human heart rhythm of the heart disease, also provide a powerful research and judge instrument for the research and development of human heart class medicine.
6. The utility model discloses can accurate discernment and provide heartbeat and rhythm of the heart curve, have advantages such as real-time supervision, quick, easy and simple to handle, be expected to play important effect in the prevention and the treatment of human heart rate disease, have wide commercial application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Figure 1 the attached drawing is the utility model provides a real-time detection and analysis zebra fish rhythm of heart's fiber arrangement's schematic structure diagram.
Wherein:
1 is a light source; 2 is a first optical fiber bundle; 3 is a fixed groove; 4 is a metal shell; 5 is zebra fish; 6 is a temperature sensor; 7 is a second optical fiber bundle; 8 is a spectrometer; 9 is a signal acquisition module; 10 is A/D conversion module; 11 is a singlechip; 12 is a display screen; 13 is a detection signal input port; and 14, a detection signal output port.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to the accompanying drawing 1, the embodiment of the utility model discloses an optical fiber device for real-time detection and analysis of zebra fish heart rate, include:
a fixing groove 3; the fixing groove 3 is used for placing and fixing zebra fish 5; the top end of the fixed slot 3 is provided with a detection signal input port 13, and the bottom end is provided with a detection signal output port 14 corresponding to the detection signal input port 13;
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port 13;
an optical signal receiving and processing terminal; the optical signal receiving and processing end is connected with the detection signal output port 14, and performs algorithm processing on the received optical signal, so as to obtain the heart rate of the zebra fish 5.
In order to further optimize the technical scheme, the metal shell 4 wraps the outer side of the fixing groove 3.
In order to further optimize the technical scheme, the optical signal transmitting end comprises a light source 1 and a first optical fiber bundle 2 connected with the transmitting end of the light source 1; the end of the first optical fiber bundle 2 far away from the light source 1 is connected with a detection signal input port 13.
In order to further optimize the technical scheme, the optical signal receiving and processing end comprises a second optical fiber bundle 7 connected with the detection signal output port 14, a spectrometer 8 connected with one end, far away from the detection signal output port 14, of the second optical fiber bundle 7, and a signal acquisition module 9, an a/D conversion module 10, a single chip microcomputer 11 and a liquid crystal display screen 12 which are electrically connected with the output port of the spectrometer 8 in sequence.
In order to further optimize the technical scheme, the optical signal receiving and processing end further comprises a temperature sensor 6 arranged inside the fixing groove 3; the temperature sensor 6 is electrically connected with the signal acquisition module 9 through a signal wire.
In order to further optimize the technical scheme, the heart of the zebra fish 5 fixed in the fixing groove 3 is positioned in the light spot range led in by the detection signal input port 13, and the eyes of the zebra fish 5 are positioned outside the light spot range led in by the detection signal input port 13.
The method for detecting and analyzing the heart rate of the zebra fish in real time by adopting the optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time comprises the following steps:
the heart rate of the zebra fish 5 is calculated by leading the first optical fiber bundle 2 into the light source 1, irradiating the heart of the zebra fish 5 fixed in the fixed groove 3, receiving and leading out the irradiated optical signal through the second optical fiber bundle 7, receiving the optical signal by the spectrometer 8, connecting the output port of the spectrometer 8 with the signal acquisition module 9, connecting the signal acquisition module 9 with the A/D conversion module 10, connecting the A/D conversion module 10 with the singlechip 11, carrying out algorithm processing on the fed-back signal by the singlechip 11, calculating the heart rate of the zebra fish 5 by adopting a frame brightness algorithm or a variable pixel intensity algorithm, and transmitting the heart rate to the liquid crystal display 12 to display the heart rate graph of the zebra fish 5.
The zebra fish is non-labeled zebra fish or zebra fish labeled by a luminous group or a fluorescent group; the spectrum signal tested by the spectrometer comprises a signal caused by the heart beat of non-labeled zebra fish or a luminescent signal or a fluorescent signal caused by the heart beat of the zebra fish marked by a luminescent group or a fluorescent group.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. An optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, which is characterized by comprising:
a fixing groove (3); the fixing groove (3) is used for placing and fixing zebra fish (5); the top end of the fixed groove (3) is provided with a detection signal input port (13), and the bottom end of the fixed groove is provided with a detection signal output port (14) corresponding to the detection signal input port (13);
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port (13);
an optical signal receiving and processing terminal; the optical signal receiving and processing end is connected with the detection signal output port (14) and performs algorithm processing on the received optical signal so as to obtain the heart rate of the zebra fish (5).
2. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1, wherein the fixing groove (3) is wrapped with a metal shell (4).
3. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1 or 2, wherein the optical signal transmitting end comprises a light source (1) and a first optical fiber bundle (2) connected with the transmitting end of the light source (1); one end, far away from the light source (1), of the first optical fiber bundle (2) is connected with the detection signal input port (13).
4. The optical fiber device for detecting and analyzing the heart rate of zebra fish in real time as claimed in claim 3, wherein the optical signal receiving and processing end comprises a second optical fiber bundle (7) connected with the detection signal output port (14), a spectrometer (8) connected with one end of the second optical fiber bundle (7) far away from the detection signal output port (14), and a signal acquisition module (9), an A/D conversion module (10), a single chip microcomputer (11) and a liquid crystal display (12) which are sequentially and electrically connected with the output port of the spectrometer (8).
5. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 4, wherein the optical signal receiving and processing end further comprises a temperature sensor (6) installed inside the fixing groove (3); the temperature sensor (6) is electrically connected with the signal acquisition module (9) through a signal wire.
6. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1, wherein the heart of the zebra fish (5) fixed in the fixing groove (3) is positioned in the light spot range introduced by the detection signal input port (13), and the eyes of the zebra fish (5) are positioned outside the light spot range introduced by the detection signal input port (13).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122990787.9U CN218588996U (en) | 2021-12-01 | 2021-12-01 | Optical fiber device for detecting and analyzing heart rate of zebra fish in real time |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202122990787.9U CN218588996U (en) | 2021-12-01 | 2021-12-01 | Optical fiber device for detecting and analyzing heart rate of zebra fish in real time |
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| CN218588996U true CN218588996U (en) | 2023-03-10 |
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| CN202122990787.9U Active CN218588996U (en) | 2021-12-01 | 2021-12-01 | Optical fiber device for detecting and analyzing heart rate of zebra fish in real time |
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Effective date of registration: 20230630 Address after: 250103 Caishi Town, Licheng District, Jinan City, Shandong Province, 28789 Jingshidong Road Patentee after: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES Patentee after: SHANDONG HONGJITANG PHARMACEUTICAL GROUP Co.,Ltd. Address before: 250103 Caishi Town, Licheng District, Jinan City, Shandong Province, 28789 Jingshidong Road Patentee before: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES |