CN114010176A - Optical fiber device and method for detecting and analyzing heart rate of zebra fish in real time - Google Patents
Optical fiber device and method for detecting and analyzing heart rate of zebra fish in real time Download PDFInfo
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- A—HUMAN NECESSITIES
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/02—Detecting, measuring or recording pulse, heart rate, blood pressure or blood flow; Combined pulse/heart-rate/blood pressure determination; Evaluating a cardiovascular condition not otherwise provided for, e.g. using combinations of techniques provided for in this group with electrocardiography or electroauscultation; Heart catheters for measuring blood pressure
- A61B5/024—Detecting, measuring or recording pulse rate or heart rate
- A61B5/02416—Detecting, measuring or recording pulse rate or heart rate using photoplethysmograph signals, e.g. generated by infrared radiation
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Abstract
The invention discloses an optical fiber device and a method for detecting and analyzing the heart rate of zebra fish in real time; the device comprises a fixed groove, an optical signal transmitting end and an optical signal receiving and processing end; the fixing groove is used for placing and fixing zebra fish; the top end of the fixed groove is provided with a detection signal input port, and the bottom end of the fixed groove is provided with a detection signal output port corresponding to the detection signal input port; the optical signal transmitting end is connected with the detection signal input port; the optical signal receiving and processing end is connected with the detection signal output port. The heart of the zebra fish is irradiated by an optical fiber lead-in light source, the irradiated light signal is received by a spectrometer, and then the signal output by the spectrometer is subjected to algorithm calculation to obtain the heart rate of the zebra fish. Compared with the common microscope method, the method greatly reduces the error caused by manual operation and can obviously enhance the accuracy of heart rate measurement.
Description
Technical Field
The invention relates to the technical field of optical fiber sensing, in particular to an optical fiber device and an optical fiber method for detecting and analyzing the heart rate of zebra fish in real time.
Background
The zebra fish is a common tropical fish, the gene of the zebra fish has 87% high similarity with the human gene, and the experimental result obtained by the drug experiment on the zebra fish is also suitable for the human body in most cases. Moreover, the zebra fish has strong reproductive capacity, short reproduction period and large egg laying amount, and the fertilized eggs can hatch the fry after 2-3 days. In addition, the zebra fish is small and exquisite in individual, convenient to culture, transparent in embryo body and easy to observe the influence of the medicine on the internal organs of the zebra fish. Therefore, the zebra fish can be used as a model of human diseases and used for judging the action and effect of the medicament on human treatment.
Heart disease is a very complex disease, and heart rate is the most intuitive reflection of whether the heart is functioning normally or not. For people at high risk of cardiovascular disease, long-term continuous monitoring of heart rate can establish a first line of defense against heart attack. By continuously and accurately monitoring and analyzing the heart rate in real time, abnormal heart activity can be found in time, and precious diagnosis and intervention time is won for patients. Due to the high similarity of zebra fish and human genes, monitoring the heart rate variation of zebra fish can provide reference for health monitoring of human heart. Through the real-time measurement of the zebra fish heart rate, very useful health information can be accurately calculated, the human heart disease can be prejudged, and the human heart disease can be treated and prevented in a targeted manner as soon as possible, so that the real-time measurement of the zebra fish heart rate has important significance for researching the prevention and treatment of the human heart disease.
At present, many researchers have carried out the research on the heart rate of the zebra fish, for example, the research on the embryonic development and the cardiac toxicity of the zebra fish by arecoline hydrobromide, which is a consortium of jia tai and dong haipeng, etc., which is published in 2021.14(07) of global traditional Chinese medicine, the research on the toxic effect of bisphenol AP on the cardiac development of the zebra fish, which is a consortium of wangthogh, caocheng, etc., is published in 2019.42(08) of environmental science and technology, and the research on the embryonic development of the zebra fish by isoliquiritigenin, which is a consortium of shang hong, caocheng, etc., is published in 2018.37(06) of sichuan animal, angiogenesis and cardiac influence, the influence of different black cis-lobe alcohol extracts on the toxicity and heart rate of zebra fish by the book of jundawn and mullein was published in 2021.39(01), and the recognition and monitoring method of the heart of zebra fish juvenile fish in micromanipulation by the book of Ming and Huanggang was published in 2020.52(06), which is published in Haerbin Industrial university Proc. In the process of monitoring the heart rate of the zebra fish, the researchers adopt a microscope observation method. However, since the heart diameter of zebra fish is generally about 100 μm, the method of microscopic observation has certain difficulties in operation and observation, and is easily affected by adverse factors such as artificial operation and environmental noise.
Therefore, how to provide a simple and effective optical fiber device and method for detecting and analyzing the heart rate of zebra fish in real time is a problem to be solved by those skilled in the art.
Disclosure of Invention
In view of this, the present invention provides an optical fiber apparatus and method for detecting and analyzing the heart rate of zebra fish in real time, and aims to solve the above technical solutions.
In order to achieve the purpose, the invention adopts the following technical scheme:
an optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, comprising:
fixing grooves; the fixing groove is used for placing and fixing zebra fish; the top end of the fixed groove is provided with a detection signal input port, and the bottom end of the fixed groove is provided with a detection signal output port corresponding to the detection signal input port;
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port;
an optical signal receiving and processing terminal; and the optical signal receiving and processing end is connected with the detection signal output port and is used for carrying out algorithm processing on the received optical signals so as to obtain the heart rate of the zebra fish.
Through the technical scheme, the optical fiber device provided by the invention adopts a mode of fixing the zebra fish, and utilizes the optical signal transmitting end and the optical signal receiving and processing end to perform optical signal processing on the zebra fish, so that the zebra fish can be ensured to monitor the heart rate change of the zebra fish in real time under the undamaged condition.
Preferably, in the optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the fixing groove is wrapped with a metal shell. By using the metal closed box, the interference of adverse factors such as environmental noise and the like is effectively avoided or weakened; the fixing groove is a cuboid fixing groove with the volume slightly larger than that of the zebra fish.
Preferably, in the above optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the optical signal transmitting end includes a light source, and a first optical fiber bundle connected to the transmitting end of the light source; and one end of the first optical fiber bundle, which is far away from the light source, is connected with the detection signal input port. The light signal output of the light source can be satisfied.
Preferably, in the above optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, the optical signal receiving and processing end includes a second optical fiber bundle connected to the detection signal output port, a spectrometer connected to one end of the detection signal output port and a signal acquisition module, an a/D conversion module, a single chip microcomputer and a liquid crystal display screen, which are electrically connected to the output port of the spectrometer in sequence. The singlechip carries out algorithm processing on the fed-back signals and transmits the signals to the liquid crystal display screen to display the heart rate graph of the zebra fish.
Preferably, in the above optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time, the optical signal receiving and processing end further includes a temperature sensor installed inside the fixing groove; the temperature sensor is electrically connected with the signal acquisition module through a signal wire and can detect the temperature in the fixed groove.
Preferably, in the above optical fiber apparatus for detecting and analyzing the heart rate of the zebra fish in real time, the heart of the zebra fish fixed in the fixing groove is located within the light spot range introduced by the detection signal input port, and the eyes of the zebra fish are located outside the light spot range introduced by the detection signal input port. Can detect the zebra fish rhythm of the heart, and prevent to cause the damage to its eyes.
The invention also provides a method for detecting and analyzing the heart rate of the zebra fish in real time, wherein a light source is led in through the optical fiber to irradiate the heart part of the zebra fish fixedly, the irradiated optical signal is received through a spectrometer, and then the signal output by the spectrometer is subjected to algorithm calculation to obtain the heart rate of the zebra fish.
Through the technical scheme, the method provided by the invention does not need to implant electrodes, utilizes the optical signal to carry out detection, greatly reduces errors caused by manual operation compared with a common microscopic method, and can obviously enhance the accuracy of heart rate measurement.
Preferably, in the method for detecting and analyzing the heart rate of the zebra fish in real time, the algorithm adopts a frame brightness algorithm or a variable pixel intensity algorithm. Tracking the change of the average light intensity of each frame of image by adopting a frame brightness algorithm, and calculating the heart rate; or a variable pixel intensity algorithm to detect heart rate by comparing the intensity variation of pixels from one frame to the next.
Preferably, in the method for detecting and analyzing the heart rate of the zebra fish in real time, the zebra fish is non-labeled zebra fish or zebra fish labeled with a luminescent group or a fluorescent group; the spectrum signal tested by the spectrometer comprises a signal caused by the heart beat of non-labeled zebra fish or a luminescent signal or a fluorescent signal caused by the heart beat of the zebra fish marked by a luminophore or a fluorophore. The detection applicability is stronger.
Preferably, in the method for detecting and analyzing the heart rate of the zebra fish in real time, the zebra fish used for detection is in a fixed state or a sleeping state, so that the influence of factors such as swimming around the zebra fish, water flow and the like is avoided, and the actual heart rate of the zebra fish can be more accurately measured.
According to the technical scheme, compared with the prior art, the invention discloses the optical fiber device and the method for detecting and analyzing the heart rate of the zebra fish in real time, and the optical fiber device and the method have the following beneficial effects:
1. according to the invention, an electrode is not required to be implanted, and the heart rate change of the zebra fish can be monitored in real time under the undamaged condition.
2. The invention effectively avoids or weakens the interference of adverse factors such as environmental noise and the like by using the metal closed box.
3. The method adopts a mode of fixing the zebra fish, avoids the influence of factors such as swimming of the zebra fish, water flow and the like, and can more accurately measure the actual heart rate of the zebra fish.
4. Compared with the common microscope method, the method greatly reduces the error caused by manual operation and can obviously enhance the accuracy of heart rate measurement.
5. The invention provides a certain reference for researching the influence of the change of the external environment on the human heart and judging the heart rate diseases of the human heart, and also provides a powerful tool for researching and developing human heart medicaments.
6. The invention can accurately identify and provide heartbeat and heart rate curves, has the advantages of real-time monitoring, rapidness, simple and convenient operation and the like, is expected to play an important role in preventing and treating heart rate diseases of human bodies, and has wide commercial application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of an optical fiber device for detecting and analyzing the heart rate of zebra fish in real time provided by the invention.
Wherein:
1 is a light source; 2 is a first optical fiber bundle; 3 is a fixed groove; 4 is a metal shell; 5 is zebra fish; 6 is a temperature sensor; 7 is a second optical fiber bundle; 8 is a spectrometer; 9 is a signal acquisition module; 10 is A/D conversion module; 11 is a singlechip; 12 is a display screen; 13 is a detection signal input port; and 14 is a detection signal output port.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to the attached drawing 1, the embodiment of the invention discloses an optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, which comprises:
a fixed groove 3; the fixing groove 3 is used for placing and fixing zebra fish 5; the top end of the fixed groove 3 is provided with a detection signal input port 13, and the bottom end is provided with a detection signal output port 14 corresponding to the detection signal input port 13;
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port 13;
an optical signal receiving and processing terminal; the optical signal receiving and processing end is connected with the detection signal output port 14, and performs algorithm processing on the received optical signal, so as to obtain the heart rate of the zebra fish 5.
In order to further optimize the technical scheme, the outer side of the fixing groove 3 is wrapped with a metal shell 4.
In order to further optimize the technical scheme, the optical signal transmitting end comprises a light source 1 and a first optical fiber bundle 2 connected with the transmitting end of the light source 1; the end of the first optical fiber bundle 2 away from the light source 1 is connected to the detection signal input port 13.
In order to further optimize the technical scheme, the optical signal receiving and processing end comprises a second optical fiber bundle 7 connected with the detection signal output port 14, a spectrometer 8 connected with one end, far away from the detection signal output port 14, of the second optical fiber bundle 7, and a signal acquisition module 9, an a/D conversion module 10, a single chip microcomputer 11 and a liquid crystal display screen 12 which are sequentially and electrically connected with the output port of the spectrometer 8.
In order to further optimize the technical scheme, the optical signal receiving and processing end further comprises a temperature sensor 6 arranged inside the fixing groove 3; the temperature sensor 6 is electrically connected with the signal acquisition module 9 through a signal wire.
In order to further optimize the technical scheme, the heart of the zebra fish 5 fixed in the fixing groove 3 is positioned in the light spot range led in by the detection signal input port 13, and the eyes of the zebra fish 5 are positioned outside the light spot range led in by the detection signal input port 13.
The method for detecting and analyzing the heart rate of the zebra fish in real time by adopting the optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time comprises the following steps:
the heart rate of the zebra fish 5 is calculated by leading the first optical fiber bundle 2 into the light source 1, irradiating the heart of the zebra fish 5 fixed in the fixed groove 3, receiving and leading out the irradiated optical signal through the second optical fiber bundle 7, receiving the optical signal by the spectrometer 8, connecting the output port of the spectrometer 8 with the signal acquisition module 9, connecting the signal acquisition module 9 with the A/D conversion module 10, connecting the A/D conversion module 10 with the singlechip 11, carrying out algorithm processing on the fed-back signal by the singlechip 11, calculating the heart rate of the zebra fish 5 by adopting a frame brightness algorithm or a variable pixel intensity algorithm, and transmitting the heart rate to the liquid crystal display 12 to display the heart rate graph of the zebra fish 5.
The zebra fish is non-labeled zebra fish or zebra fish labeled by a luminous group or a fluorescent group; the spectrum signal tested by the spectrometer comprises a signal caused by the heart beat of non-labeled zebra fish or a luminescence signal or a fluorescence signal caused by the heart beat of the zebra fish marked by luminophores or fluorophores.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. An optical fiber device for detecting and analyzing the heart rate of zebra fish in real time, which is characterized by comprising:
a fixing groove (3); the fixing groove (3) is used for placing and fixing zebra fish (5); the top end of the fixed groove (3) is provided with a detection signal input port (13), and the bottom end of the fixed groove is provided with a detection signal output port (14) corresponding to the detection signal input port (13);
an optical signal transmitting terminal; the optical signal transmitting end is connected with the detection signal input port (13);
an optical signal receiving and processing terminal; the optical signal receiving and processing end is connected with the detection signal output port (14) and performs algorithm processing on the received optical signal so as to obtain the heart rate of the zebra fish (5).
2. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1, wherein the fixing groove (3) is wrapped with a metal shell (4).
3. The optical fiber device for detecting and analyzing the heart rate of zebra fish in real time according to claim 1 or 2, wherein the optical signal transmitting end comprises a light source (1) and a first optical fiber bundle (2) connected with the transmitting end of the light source (1); one end, far away from the light source (1), of the first optical fiber bundle (2) is connected with the detection signal input port (13).
4. The optical fiber device for detecting and analyzing the heart rate of zebra fish in real time as claimed in claim 3, wherein the optical signal receiving and processing end comprises a second optical fiber bundle (7) connected with the detection signal output port (14), a spectrometer (8) connected with one end of the second optical fiber bundle (7) far away from the detection signal output port (14), and a signal acquisition module (9), an A/D conversion module (10), a single chip microcomputer (11) and a liquid crystal display (12) which are sequentially and electrically connected with the output port of the spectrometer (8).
5. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 4, wherein the optical signal receiving and processing end further comprises a temperature sensor (6) installed inside the fixing groove (3); the temperature sensor (6) is electrically connected with the signal acquisition module (9) through a signal wire.
6. The optical fiber device for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1, wherein the heart of the zebra fish (5) fixed in the fixing groove (3) is positioned in the light spot range introduced by the detection signal input port (13), and the eyes of the zebra fish (5) are positioned outside the light spot range introduced by the detection signal input port (13).
7. A method for detecting and analyzing the heart rate of zebra fish in real time is characterized in that a light source is led in through an optical fiber to irradiate a fixed heart part of the zebra fish, an irradiated optical signal is received through a spectrometer, and then an algorithm calculation is carried out on a signal output by the spectrometer, so that the heart rate of the zebra fish is obtained.
8. The method for detecting and analyzing the heart rate of the zebra fish in real time according to claim 1, wherein the algorithm adopts a frame brightness algorithm or a variable pixel intensity algorithm.
9. The method for detecting and analyzing the heart rate of the zebra fish in real time according to claim 1, wherein the zebra fish is non-labeled zebra fish or zebra fish labeled with a luminophore or a fluorophore; the spectrum signal tested by the spectrometer comprises a signal caused by the heart beat of non-labeled zebra fish or a luminescent signal or a fluorescent signal caused by the heart beat of the zebra fish marked by a luminophore or a fluorophore.
10. The method for detecting and analyzing the heart rate of the zebra fish in real time as claimed in claim 1, wherein the zebra fish for detection is in a fixed state or a sleeping state.
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| CN202111453804.3A CN114010176A (en) | 2021-12-01 | 2021-12-01 | Optical fiber device and method for detecting and analyzing heart rate of zebra fish in real time |
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| CN202111453804.3A CN114010176A (en) | 2021-12-01 | 2021-12-01 | Optical fiber device and method for detecting and analyzing heart rate of zebra fish in real time |
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