CN218584807U - Endotoxin detection assembly and detection device for collagen endotoxin - Google Patents

Abstract

The application provides an endotoxin detection subassembly and be used for detection device of collagen endotoxin relates to endotoxin check out test set technical field. The endotoxin detection assembly comprises a detection main body and a detection reagent liquid supply mechanism, wherein the detection main body is made of transparent materials, and a plurality of first flow channels, a plurality of second flow channels, a plurality of third flow channels and a plurality of fourth flow channels which are arranged at intervals are arranged in the detection main body; the first flow channel is communicated with the fourth flow channel so as to supply the test solution to the fourth flow channel; the second flow channel and the fourth flow channel connected, to the fourth flow channel for supplying endotoxin test water; the third flow channel is communicated with the fourth flow channel so as to supply bacterial endotoxin standard solution to the fourth flow channel; the detection reagent supply mechanism is configured to supply the limulus reagent to the plurality of fourth flow channels. The application of endotoxin detection subassembly and be used for the detection device of collagen endotoxin can raise the efficiency when being used for endotoxin to detect.

Description

Endotoxin detection assembly and detection device for collagen endotoxin

Technical Field

The application relates to the technical field of endotoxin detection equipment, in particular to an endotoxin detection assembly and a detection device for collagen endotoxin.

Background

Bacterial endotoxins are cell wall components of gram-negative bacteria that release endotoxins when the bacteria die or autolyze. Endotoxin does not cause any harm when it enters the human body through the digestive tract, but endotoxin causes various diseases when it enters the blood by injection or the like. After a small amount of endotoxin enters the blood, the endotoxin is inactivated by kupffer cells of the liver, so that the damage to the organism is not caused. However, if a large amount of endotoxin enters the blood, a fever reaction, a "pyrogen reaction" is caused. Therefore, preparations such as biological products, injection medicines, chemical medicines, radiopharmaceuticals, antibiotics, vaccines, dialyzates and medical instruments (such as disposable syringes and implantable biomaterials) can be used after being qualified by bacterial endotoxin detection tests.

In the prior art, a limulus reagent is generally adopted to detect bacterial endotoxin, the limulus reagent and the bacterial endotoxin can generate agglutination reaction, the bacterial endotoxin can be effectively detected through the limulus reagent, when the endotoxin content in a sample is greater than the sensitivity indicated value of the limulus reagent, the limulus reagent is added into the sample to form gel, and if the endotoxin content in the sample is less than the sensitivity indicated value of the limulus reagent, the limulus reagent is added into the sample to form no gel. In order to improve the effectiveness of endotoxin detection, a positive control, a test sample positive control and a negative control are generally added to carry out an experiment together, and the whole endotoxin detection experiment is effective only when the positive control and the test sample positive control are positive and the negative control is negative. When the limulus reagent is used for detecting endotoxin, the test tubes are generally used for holding samples, each test tube can only hold one sample, each sample needs to be subjected to sample configuration, the sample configurations of a positive reference substance, a test sample positive reference substance, a negative reference substance and the test sample are different, a worker needs to sequentially configure a plurality of groups of samples in the experiment process, and the efficiency is low.

SUMMERY OF THE UTILITY MODEL

The application provides an endotoxin determine module and be used for the detection device of collagen endotoxin, it can raise the efficiency.

The application is realized as follows:

in a first aspect, the present application provides an endotoxin detection module comprising:

the detection main body is made of transparent materials, a plurality of first flow channels, a plurality of second flow channels, a plurality of third flow channels and a plurality of fourth flow channels are arranged in the detection main body at intervals, and the bottom end of each fourth flow channel is closed; the first flow channel is communicated with the fourth flow channel, and the first flow channel is configured as a liquid supply flow channel of the test solution so as to supply the test solution to the fourth flow channel; a second flow channel communicating with the fourth flow channel, the second flow channel being configured as a liquid supply flow channel for endotoxin test water to supply the fourth flow channel with endotoxin test water; the third flow channel is communicated with the fourth flow channel, and the third flow channel is configured as a liquid supply channel of the bacterial endotoxin standard solution so as to supply the bacterial endotoxin standard solution to the fourth flow channel;

a detection reagent supply mechanism configured to supply a limulus reagent to the plurality of fourth flow paths.

In a possible embodiment, the detection reagent supply mechanism comprises a liquid supply container and a liquid outlet pipe, the liquid outlet pipe is communicated with the liquid supply container, the liquid outlet pipe is provided with a plurality of liquid outlet branch pipes, each liquid outlet branch pipe is detachably connected with the corresponding fourth flow channel, and the liquid outlet branch pipes are provided with flow regulating valves.

In a possible embodiment, one end of the liquid outlet branch pipe, which is used for being connected with the fourth flow channel, is a connecting end, the connecting end is a rubber pipe, and the rubber pipe can be inserted into the fourth flow channel.

In a possible embodiment, the inlets of the first flow channels, the second flow channels and the third flow channels are all disposed on the same side of the detection main body, the first flow channels are arranged along the length direction of the detection main body, the second flow channels are arranged along the length direction of the detection main body, and the third flow channels are arranged along the length direction of the detection main body.

In one possible embodiment, the first flow channel, the second flow channel, and the third flow channel are provided on three different sides of the detection body.

In a possible embodiment, the ends of the first flow channel, the second flow channel and the third flow channel connected to the fourth flow channel are all disposed on the middle portion of the fourth flow channel.

In a second aspect, the present application provides a device for detecting endotoxin in collagen, which comprises the endotoxin detecting assembly of the first aspect, and a collagen gel leaching solution supply mechanism configured to supply a leaching solution of collagen gel into a first flow channel; the outlet end of the collagen gel leaching liquor supply mechanism is detachably connected with the first flow channel;

an endotoxin inspection water supply mechanism configured to supply endotoxin inspection water into the second flow channel, and an outlet end of the endotoxin inspection water supply mechanism is detachably connected to the second flow channel;

and the endotoxin standard solution supply mechanism is configured to supply endotoxin standard solution into the third flow channel, and the outlet end of the endotoxin standard solution is detachably connected with the third flow channel.

In one possible embodiment, the collagen gel leach solution supply mechanism comprises a first supply body and a first supply tube; the first liquid supply pipe is detachably connected with the first flow passage and is communicated with the first flow passage; first confession liquid pipe and the inside intercommunication of first confession liquid main part, the outer wall detachably parcel of first confession liquid main part has the heating jacket.

In a possible embodiment, the heating jacket includes a heating jacket main body and a first connecting portion and a second connecting portion which are arranged at two ends of the heating jacket main body, the first connecting portion is provided with a plurality of groups of connecting holes along the circumferential direction, the second connecting portion is connected with a connecting rod body which is used for being matched with the connecting holes, and the first connecting portion and the second connecting portion can be connected through the connecting rod body which is arranged in a penetrating manner in the connecting holes.

In a possible embodiment, the first supply tube is provided with a first pumping mechanism.

The application has at least the following beneficial effects:

the utility model provides an endotoxin detection component, its inside first runner that has of main part of detection, the second runner, the fourth runner that third runner and a plurality of interval set up, can supply the test article solution to the fourth runner through first runner, can supply endotoxin inspection water to the fourth runner through the second runner, can supply bacterium endotoxin standard substance solution to the fourth runner through the third runner, endotoxin inspection water can be used as negative control article, bacterium endotoxin standard substance solution + endotoxin inspection water is compounded and can be used as positive control article, bacterium endotoxin standard substance solution + test article solution is compounded and can be used as the positive control article of test article, through selectively to the runner confession liquid in the first runner that corresponds the intercommunication with every fourth runner, among the second runner and the shunt in the third runner, can dispose the test article solution simultaneously, positive control article, the positive control article of test article and negative control article, thereby can raise the efficiency.

After the test solution, the positive reference substance, the test positive reference substance and the negative reference substance are prepared, the limulus reagent is supplied to the fourth flow channel through the detection reagent supply mechanism, the temperature is kept for about 60min, and whether the endotoxin detection result is positive or negative is judged by observing whether the sample in the fourth flow channel forms gel or not. Because the detection main part of this application is transparent material, then observe the judgement more easily during the judgement.

The utility model provides a detection apparatus for be used for collagen endotoxin, the leaching liquor that supplies the collagen gel to first runner in through collagen gel leaching liquor confession liquid mechanism has avoided the heat preservation step in the endotoxin testing process to make collagen itself form the gel and form false positive, has reduced the probability of false positive result.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive effort.

FIG. 1 is a schematic view of a partial structure of an endotoxin detection module according to an embodiment of the present application;

FIG. 2 is a schematic structural diagram of a detection body according to an embodiment of the present application;

FIG. 3 is a schematic view of another structure of the detecting body according to the embodiment of the present application;

FIG. 4 is a schematic view of a part of the structure of a detecting device according to an embodiment of the present application;

fig. 5 is a schematic structural view of the region a in fig. 4.

An icon: 10-a detection device; 100-an endotoxin detection module; 110-a detector reagent supply mechanism; 111-a liquid supply container; 112-a liquid inlet pipe; 113-a liquid outlet pipe; 1131, discharging liquid from a branch pipe; 1132-connection end; 114-flow regulating valve; 120-a detection subject; 121-a first flow channel; 122-a second flow channel; 123-a third flow channel; 124-a fourth flow channel; 200-a collagen gel leaching liquor supply mechanism; 210-a first liquid supply body; 220-a first supply tube; 221-a first pumping mechanism; 222-a first branch liquid supply; 230-a heating jacket; 231-heating jacket main body; 232-a first connection; 2321-connecting hole; 233-a second connecting portion; 234-connecting rod body.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. The components of the embodiments of the present application, as generally described and illustrated in the figures herein, could be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present application, presented in the accompanying drawings, is not intended to limit the scope of the claimed application, but is merely representative of selected embodiments of the application. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined or explained in subsequent figures.

In the description of the present application, it should be noted that the terms "upper", "lower", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships conventionally placed when products of the application are used, and are only used for convenience of description and simplification of the description, but do not indicate or imply that the devices or elements referred to must have specific orientations, be constructed in specific orientations, and be operated, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another, and are not to be construed as indicating or implying relative importance.

In the description of the present application, it is further noted that, unless expressly stated or limited otherwise, the terms "disposed," "mounted," and "connected" are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in this application will be understood to be a specific case for those of ordinary skill in the art.

Examples

The present embodiment provides an endotoxin detecting assembly 100, please refer to fig. 1, which comprises a detecting main body 120 for detecting endotoxin and a detecting reagent supply mechanism 110, wherein the detecting reagent for detecting endotoxin is limulus reagent.

The detection main body 120 is made of a transparent material, and has a plurality of first flow channels 121, a plurality of second flow channels 122, a plurality of third flow channels 123, and a plurality of fourth flow channels 124 (see fig. 2 and 3) arranged at intervals therein, wherein a bottom end of the fourth flow channel 124 is closed.

The first channel 121 is communicated with the fourth channel 124, the first channel 121 is configured as a supply channel for the sample solution, and the sample solution can be supplied to the fourth channel 124 through the first channel 121. The second flow channel 122 communicates with the fourth flow channel 124, and the second flow channel 122 is configured as a liquid supply flow channel for endotoxin test water to supply the fourth flow channel 124 with the endotoxin test water. The third flow channel 123 is communicated with the fourth flow channel 124, and the third flow channel 123 is configured as a liquid supply flow channel of the bacterial endotoxin standard solution to supply the bacterial endotoxin standard solution to the fourth flow channel 124.

When endotoxin detection is carried out, 4 groups of samples are generally required to be arranged, and each group of samples comprises at least two parallel samples so as to improve the detection accuracy. The 4 groups of samples are respectively a test solution, a positive reference substance, a test positive reference substance and a test negative reference substance. The test solution is a sample to be tested, the positive reference substance, the test positive reference substance and the negative reference substance are all used as reference substances, and the endotoxin detection result of the test solution is effective only when the endotoxin detection results of the positive reference substance and the test positive reference substance are positive and the endotoxin detection result of the negative reference substance is negative.

Wherein, the water for the bacterial endotoxin can be used as a negative control substance, the combination of the bacterial endotoxin standard substance solution and the endotoxin detection water can be used as a positive control substance, and the combination of the bacterial endotoxin standard substance solution and the test sample solution can be used as a positive control substance of the test sample. Because each fourth flow channel 124 is correspondingly communicated with the first flow channel 121, the second flow channel 122 and the third flow channel 123, liquid can be selectively supplied to partial flow channels among the first flow channel 121, the second flow channel 122 and the third flow channel 123 which are correspondingly communicated with each fourth flow channel 124, and the solutions supplied to different fourth flow channels 124 are different, so that a sample solution, a positive control, a sample positive control and a negative control are respectively configured in a plurality of fourth flow channels 124 of the detection main body 120, a worker does not need to sequentially configure a plurality of groups of samples, and the efficiency is higher.

After the test solution, the positive control and the negative control of the test sample are prepared, the limulus reagent is supplied to the fourth flow channel 124 through the detection reagent supply mechanism 110, the temperature is kept for about 60min, and whether the endotoxin detection result is positive or negative is judged by observing whether the sample in the fourth flow channel 124 forms gel. Because the detection main part 120 of this application is transparent material, then observe the judgement more easily during the judgement, for example, the material can be transparent glass material or transparent high temperature resistant plastics material. For example, the detection body 120 of the embodiment of the present application may be a disposable article, and when performing endotoxin detection next time, the detection body 120 may be replaced with a new one, and before use, the detection body 120 should be treated to remove exogenous endotoxin, for example, the detection body 120 made of a heat-resistant material may be treated by dry heat sterilization (250 ℃, at least 30 min).

When determining the endotoxin detection result, after the sample is prepared, the first flow channel 121, the second flow channel 122, the third flow channel 123 and the fourth flow channel 124 of the detection main body 120 are sealed, then the detection main body 120 is placed in a 37 ± 1 ℃ drying oven for heat preservation for about 60min, and whether the sample in the fourth flow channel 124 forms gel is observed by slowly inverting the detection main body 120 by 180 °. If the gel is formed in the fourth flow channel 124 and the gel is not deformed, the one that does not slip off the inner wall of the fourth flow channel 124 is positive and is recorded as (+); the person who did not form gel or formed gel was not firm, deformed and slipped from the inner wall of the fourth flow channel 124 was negative and recorded as (-to) to judge the endotoxin detection result of the test article. It should be noted that, the process of performing the incubation and determining the endotoxin test result should avoid the false negative result caused by the vibration of the sample.

In some embodiments, the plurality of first flow channels 121, the plurality of second flow channels 122, and the plurality of third flow channels 123 are disposed on the same side of the detection body 120, the plurality of first flow channels 121 are arranged along the length direction of the detection body 120, the plurality of second flow channels 122 are arranged along the length direction of the detection body 120, and the plurality of third flow channels 123 are arranged along the length direction of the detection body 120 (see fig. 2). That is, the first flow channel 121, the second flow channel 122, and the third flow channel 123 which are communicated with each fourth flow channel 124 are all distributed along the height direction of the fourth flow channel 124 in the same order, for example, along the height direction of the fourth flow channel 124, the first flow channel 121, the second flow channel 122, and the third flow channel 123 are arranged in sequence; or a first channel 121, a third channel 123 and a second channel 122 in sequence; or the second flow channel 122, the first flow channel 121, and the third flow channel 123 in this order. In operation, it is more convenient to connect different liquid supply mechanisms to the first flow passage 121, the second flow passage 122 and the third flow passage 123, respectively.

In another possible embodiment, the first flow channel 121, the second flow channel 122 and the third flow channel 123 are respectively disposed on three different sides of the detection body 120 (refer to fig. 3), and the plurality of fourth flow channels 124 are disposed in a staggered manner to facilitate the arrangement of the first flow channel 121, the second flow channel 122 and the third flow channel 123. Since the first flow channel 121, the second flow channel 122 and the third flow channel 123 are respectively disposed on three sides of the detection main body 120, different liquid supply mechanisms can be disposed on different sides of the detection main body 120, and can be more conveniently connected to the first flow channel 121, the second flow channel 122 and the third flow channel 123.

It is understood that, in both embodiments, the liquid inlet of the fourth channel 124 is optionally provided on the top wall of the detection body 120 to facilitate the supply of the limulus reagent from the detection reagent supply mechanism 110. In addition, the ends of the first flow channel 121, the second flow channel 122, and the third flow channel 123 connected to the fourth flow channel 124 are all disposed on the middle portion of the fourth flow channel 124, so that the supplied solution can be better retained in the fourth flow channel 124. Note that the portion above the middle of the fourth flow channel 124 is a portion that occupies 1/2 of the height along the height direction of the fourth flow channel 124.

Further, referring to fig. 1, the detection reagent supply mechanism 110 includes a supply container 111 and a liquid outlet pipe 113, the liquid outlet pipe 113 is communicated with the supply container 111, the liquid outlet pipe 113 has a plurality of liquid outlet branches 1131, each liquid outlet branch 1131 is detachably connected to the fourth flow channel 124, and the liquid outlet branch 1131 is provided with a flow rate adjusting valve 114. The limulus reagent is supplied to the liquid supply container 111 through the first liquid supply tube 220, and then the limulus reagent is supplied to the plurality of fourth flow passages 124 through the plurality of liquid outlet branch tubes 1131, respectively, and the liquid outlet branch tubes 1131 are provided with the flow regulating valves 114, so that the amount of the limulus reagent introduced into each of the fourth flow passages 124 can be controlled by the flow regulating valves 114.

Exemplarily, one end of the liquid outlet pipe 1131 for connecting with the fourth flow channel 124 is a connection end 1132, the connection end 1132 is a rubber pipe, and the rubber pipe can be inserted into the fourth flow channel 124. The rubber tube can conveniently insert fourth runner 124, and the rubber tube is the hose, can laminate better with fourth runner 124, is difficult to the weeping.

In a second aspect, the present embodiment provides a device 10 for detecting endotoxin in collagen (see fig. 4), which includes a collagen gel leaching solution supply mechanism 200, an endotoxin test water supply mechanism (not shown), and an endotoxin standard solution supply mechanism (not shown).

The collagen gel leaching solution supply mechanism 200 is configured to supply leaching solution of the collagen gel into the first flow channel 121, and an outlet end of the collagen gel leaching solution supply mechanism 200 is detachably connected to the first flow channel 121. In some embodiments, the collagen gel leaching solution supplying mechanism 200 includes a first liquid supplying body 210 and a first liquid supplying pipe 220, the first liquid supplying pipe 220 is communicated with the inside of the first liquid supplying body 210, the first liquid supplying pipe 220 has a plurality of first liquid supplying branch pipes 222, and the first liquid supplying branch pipes 222 of the first liquid supplying pipe 220 are detachably connected to the first flow channel 121 and are communicated with the first flow channel 121. Optionally, the first branch liquid supply pipe 222 is fitted with a flow regulating valve 114 so that the amount of supply can be controlled. Illustratively, one end of the first branch liquid supply pipe 222 is a rubber hose capable of being tightly inserted into the first flow passage 121.

The outer wall of the first liquid supply body 210 may removably enclose a heating jacket 230, and the heating jacket 230 may be configured to heat the contents of the first liquid supply body 210. The present inventors considered that the incubation at 37. + -. 1 ℃ was required after adding the limulus reagent to the sample, and then the endotoxin detection results were judged based on whether the sample formed a gel. Because it is to detect endotoxin in the collagen liquid, if the collagen liquid is directly used as a sample, the collagen liquid may self-assemble to form collagen gel due to the influence of temperature, so that it cannot be determined whether the collagen liquid itself becomes gel or the endotoxin in the collagen liquid and limulus reagent undergo agglutination reaction, thereby causing a false positive result.

In the present application, the heating jacket 230 is disposed on the outer wall of the first liquid supply main body 210, and in the specific use process, the collagen liquid is first placed in the first liquid supply main body 210, and then heated by the heating jacket 230, and the collagen liquid is self-assembled to form collagen gel when being kept at 37 ± 1 ℃. After the collagen gel is formed, endotoxin test water is added to soak the collagen gel for about 24 hours, and a leaching solution of the collagen gel is supplied to the first flow path 121 through the first liquid supply tube 220. Because the sample adopts the leaching liquor of the collagen gel, but not the collagen liquid, the accuracy that the collagen liquid can form gel to influence the detection result of the endotoxin is eliminated. Illustratively, the first liquid supply pipe 220 is equipped with a first pumping mechanism 221, by which the leaching solution can be more conveniently pumped to the first flow channel 121, the first pumping mechanism 221 being optionally a peristaltic pump, a gear pump, etc. It will be appreciated that the first liquid supply body 210, the first liquid supply pipe 220 and the first branch liquid supply pipe 222 are replaceable, i.e., can be replaced with new ones for the next use. The first liquid supply body 210 may be made of plastic or glass; the material of the first liquid supply tube 220 and the first branch liquid supply tube 222 may alternatively be plastic or rubber.

Optionally, the added collagen liquid has a pH of 6 to 8, the collagen liquid contains a phosphate buffer solution, and the collagen is dissolved in the phosphate buffer solution. Among them, the phosphate buffer solution contains disodium hydrogen phosphate and sodium dihydrogen phosphate, and the inventors of the present application have found in their studies that collagen is more likely to form a collagen gel in one piece when it is dissolved in the phosphate buffer solution.

Further, referring to fig. 5, the heating jacket 230 includes a heating jacket main body 231, and a first connecting portion 232 and a second connecting portion 233 which are disposed at two ends of the heating jacket main body 231, the first connecting portion 232 is provided with a plurality of sets of connecting holes 2321 along a circumferential direction thereof, the second connecting portion 233 is connected with a connecting rod 234 for being matched with the connecting holes 2321, the first connecting portion 232 and the second connecting portion 233 can be connected by the connecting rod 234 penetrating through the connecting holes 2321, and then are in threaded connection with the connecting rod 234 by a locking nut and the like, so that the first connecting portion 232 and the second connecting portion 233 are connected and fixed. Since the first connection portion 232 is provided with a plurality of sets of connection holes 2321 along the circumferential direction thereof, the external size of the heating jacket main body 231 can be adapted by the connection rod 234 being engaged with the connection holes 2321 at different positions in the circumferential direction.

In addition, the endotoxin test water supply mechanism is configured to supply endotoxin test water into the second flow channel 122, and an outlet end of the endotoxin test water supply mechanism is detachably connected to the second flow channel 122. The endotoxin standard solution supply mechanism is configured to supply the endotoxin standard solution into the third flow channel 123, and the outlet end of the endotoxin standard solution is detachably connected to the third flow channel 123.

The endotoxin test water supply mechanism (not shown) includes a second liquid supply main body and a second liquid supply pipe communicated with the second liquid supply main body, the second liquid supply pipe has a plurality of second liquid supply branch pipes, and the second liquid supply branch pipes can be tightly inserted into the second flow channels 122 to supply endotoxin test water into the second flow channels 122. Optionally, the second liquid supply tube is equipped with a flow rate regulating valve, so that the amount of endotoxin-inspecting water supplied can be controlled. Illustratively, the second supply tube is mounted with a second pumping mechanism by which endotoxin inspection water can be more conveniently pumped to the second flow channel 122, the second pumping mechanism being optionally a peristaltic pump, a gear pump or the like.

The endotoxin standard solution supply mechanism (not shown) includes a third liquid supply main body and a third liquid supply pipe communicated with the third liquid supply main body, the third liquid supply pipe has a plurality of third liquid supply branch pipes, and the third liquid supply branch pipes can be tightly inserted into the third flow channels 123 to supply endotoxin standard solutions into the third flow channels 123. Optionally, the third liquid supply tube is equipped with a flow regulating valve so that the amount of endotoxin standard solution supplied can be controlled. Illustratively, the third supply tube is mounted with a third pumping mechanism by which endotoxin-inspecting water can be more conveniently pumped to the third flow channel 123, the third pumping mechanism being optionally a peristaltic pump, a gear pump or the like.

In summary, the endotoxin detection assembly according to the embodiment of the present application can simultaneously dispose the test solution, the positive control and the negative control of the test sample by selectively supplying the liquid to some of the first flow channel, the second flow channel and the third flow channel, which are correspondingly communicated with each of the fourth flow channels, so as to improve the efficiency. The detection device for the collagen endotoxin of the embodiment of the application supplies the leaching liquor of the collagen gel to the first flow channel through the collagen gel leaching liquor supply mechanism, avoids the heat preservation step in the endotoxin detection process to enable the collagen to form gel to form false positive, and reduces the probability of false positive results.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims (10)

1. An endotoxin detection module, comprising:

the detection main body is made of transparent materials, a plurality of first flow channels, a plurality of second flow channels, a plurality of third flow channels and a plurality of fourth flow channels are arranged in the detection main body at intervals, and the bottom end of each fourth flow channel is closed; the first flow channel is communicated with the fourth flow channel, and the first flow channel is configured as a liquid supply flow channel of a test solution so as to supply the test solution to the fourth flow channel; the second flow channel is communicated with the fourth flow channel, the second flow channel is configured to be a liquid supply flow channel of the water for endotoxin detection, so as to supply the water for endotoxin detection to the fourth flow channel; the third flow channel is communicated with the fourth flow channel, and the third flow channel is configured as a liquid supply channel of the bacterial endotoxin standard solution so as to supply the bacterial endotoxin standard solution to the fourth flow channel;

a detection reagent supply mechanism configured to supply a limulus reagent to the plurality of fourth flow paths.

2. The endotoxin detection assembly of claim 1, wherein the detection reagent supply mechanism comprises a supply container and a drain tube, the drain tube is communicated with the supply container, the drain tube is provided with a plurality of drain branches, each drain branch is detachably connected with the fourth flow channel correspondingly, and the drain branches are provided with flow control valves.

3. The endotoxin detection assembly of claim 2, wherein one end of the liquid outlet branch pipe, which is used for being connected with the fourth flow channel, is a connecting end, the connecting end is a rubber pipe, and the rubber pipe can be inserted into the fourth flow channel.

4. The endotoxin detection assembly of any one of claims 1 to 3, wherein inlets of the plurality of first channels, the plurality of second channels and the plurality of third channels are provided on the same side of the detection main body, the plurality of first channels are arranged along the length direction of the detection main body, the plurality of second channels are arranged along the length direction of the detection main body, and the plurality of third channels are arranged along the length direction of the detection main body.

5. The endotoxin detection assembly of any one of claims 1 to 3, wherein the first flow channel, the second flow channel and the third flow channel are provided on three different side surfaces of the detection main body.

6. The endotoxin detection assembly of any one of claims 1 to 3, wherein the ends of the first flow channel, the second flow channel and the third flow channel connected to the fourth flow channel are all provided on the middle part of the fourth flow channel.

7. An endotoxin-detecting instrument for collagen, which comprises the endotoxin-detecting module according to any one of claims 1 to 6, and;

a collagen gel leach solution supply mechanism configured to supply a leach solution of a collagen gel into the first flow passage; the outlet end of the collagen gel leaching liquor supply mechanism is detachably connected with the first flow channel;

an endotoxin test water supply mechanism configured to supply endotoxin test water into the second flow channel, an outlet end of the endotoxin test water supply mechanism being detachably connected to the second flow channel;

an endotoxin standard solution supply mechanism configured to supply an endotoxin standard solution into the third flow channel, and an outlet end of the endotoxin standard solution is detachably connected to the third flow channel.

8. The device for detecting endotoxin of collagen according to claim 7, wherein the collagen gel leaching solution supply mechanism comprises a first supply main body and a first supply pipe; the first liquid supply pipe is detachably connected with the first flow passage and is communicated with the first flow passage; the first liquid supply pipe is communicated with the interior of the first liquid supply main body, and the heating sleeve is detachably wrapped on the outer wall of the first liquid supply main body.

9. The device for detecting the endotoxin in collagen according to claim 8, wherein the heating jacket comprises a heating jacket main body and a first connecting portion and a second connecting portion which are arranged at two ends of the heating jacket main body, the first connecting portion is provided with a plurality of groups of connecting holes along the circumferential direction, the second connecting portion is connected with a connecting rod body matched with the connecting holes, and the first connecting portion and the second connecting portion can be connected through the connecting rod body which is arranged in a penetrating manner in the connecting holes.

10. The device for detecting endotoxin in collagen according to claim 8, wherein the first liquid supply tube is equipped with a first pumping mechanism.

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