CN218561447U - Sperm sorting device - Google Patents

Abstract

A sperm sorting device is suitable for sorting a plurality of motile sperms from a semen sample and comprises a base and a sorter fixedly connected with the base. The base includes the accommodation space that is defined out by diapire and wall around with the cooperation, the sorter includes external member and penetrating membrane, the external member has and is located the sleeve in the accommodation space, and be located the sleeve bottom and have the injection site of injection hole, it is fixed in to pass through the membrane the sleeve and with the injection site reaches the sleeve cooperatees and defines out the open separation chamber in top, pass through the membrane with the diapire reaches wall around with the cooperation defines out and is used for the holding the sample appearance room of semen physical examination body, the injection hole communicates perpendicularly the sample appearance room with the separation chamber room. Borrow this, can with the semen is examined the body certainly the injection hole pours into fast the body holds the room, makes the utility model discloses possess the operation more labour saving and time saving, overall structure is more succinct, can reduction in production cost's characteristic.

Description

Sperm sorting device

Technical Field

The utility model relates to a medical utensil is exclusively used in, especially indicates a sperm sorting unit.

Background

Referring to fig. 1, a sperm sorting system 10 of U.S. patent No. 10,422,737b2 includes a housing 12 defining an inlet 11, a collection chamber 13 disposed in the housing 12 and located adjacent to the inlet 11, a microfluidic chip 14 mounted on the underside of the housing 12, a flow channel 15 extending from the inlet 11 to the collection chamber 13, and a filter 16 disposed in the collection chamber 13, the collection chamber 13 having a bottom chamber 17 adjacent to the microfluidic chip 14, and a top chamber 18 located in the bottom chamber 17 and remote from the microfluidic chip 14. The bottom chamber 17 is used to collect a semen sample injected into the flow path 15 from the inlet 11, wherein motile sperm in the semen sample are free upward through the filter 16 into the top chamber 18, thereby achieving a sperm sorting effect.

Although the sperm sorting system 10 can sort sperm, it has the following problems:

1. because the inlet 11 is far from the bottom chamber 17 and has two turns, when a user uses a syringe to inject the semen sample from the inlet 11 into the elongated flow path 15, continuous force is required to generate sufficient pushing pressure to push the semen sample through the elongated flow path 15 and into the bottom chamber 17, which is laborious and time-consuming.

2. Since the flow path 15 must be provided, the entire structure is complicated and the production cost is high.

3. Because of the presence of the laterally disposed flow path 15 between the inlet 11 and the bottom chamber 17, the overall radial dimension of the sperm sorting system 10 needs to increase with the laterally disposed flow path 15.

Disclosure of Invention

An object of the utility model is to provide an operation is labour saving and time saving, overall structure is succinct, can reduction in production cost's sperm sorting unit.

The utility model discloses a sperm sorting device, which is suitable for sorting a plurality of movable sperms from a sperm sample, and comprises a base and a sorter;

the base comprises a bottom wall and a surrounding wall which extends from the periphery of the bottom wall to the top end and surrounds the axis, and the bottom wall and the surrounding wall are matched to define an accommodating space with an opening;

the separator is fixedly connected with the base and comprises a kit and a permeable membrane; the sleeve is provided with a sleeve positioned in the accommodating space around the axis, an extracting ring extending from the bottom end of the sleeve to the direction close to the axis, and an injection part arranged on the extracting ring, wherein the injection part is provided with an injection hole penetrating along the axis, and the sleeve is provided with an inner cylindrical surface and an outer cylindrical surface surrounding the inner cylindrical surface; the permeable membrane is fixed on the sleeve and is matched with the suction ring and the inner sleeve surface to define a separation chamber with an open top end, and the permeable membrane is provided with a plurality of micropores for the motile sperms to pass through;

the penetrating film, the bottom wall and the surrounding wall are matched to define a sample containing chamber for containing the semen sample, and the injection hole is vertically communicated with the sample containing chamber and the sorting containing chamber.

Sperm sorting unit, the base with the sorter is the integrated into one piece structure.

Sperm sorting unit, the sleeve still has the connection interior bobbin face with the bobbin base face of outer bobbin face, draw the ring have ring top surface, ring bottom surface and intercommunication the ring top surface with the centre bore of ring bottom surface, just draw the ring bottom surface of ring certainly telescopic bobbin base face orientation is close to the direction of axis extends and is close to simultaneously gradually the ring top surface.

Sperm sorting unit, draw the ring bottom surface of ring have certainly telescopic bobbin base face orientation is close to the outer extension section that the direction of axis extends, certainly outer extension section orientation is close to the linkage segment that the direction of crown face extends, and certainly the linkage segment orientation is close to the interior extension section that the direction of axis extends, the centre bore intercommunication the crown face with interior extension section of ring bottom surface, the injection hole runs through the crown face with the linkage segment of ring bottom surface, penetrating membrane is fixed in outer extension section has the correspondence the perforation of injection hole.

Sperm sorting unit, draw the ring bottom surface of ring have certainly telescopic bobbin base face is close to the outer extension section that the direction of axis extends, certainly outer extension section orientation is close to the linkage segment that the direction of ring top surface extends, and certainly the linkage segment orientation is close to the interior extension section that the direction of axis extends, the centre bore intercommunication the ring top surface with the interior extension section of ring bottom surface, the injection hole runs through the ring top surface with the outer extension section of ring bottom surface, penetrating membrane is fixed in outer extension section just is located the inboard of injection hole.

Sperm sorting unit, injection portion still have the shaping in draw the ring top face of ring and center on the lug of top of injection hole.

Sperm sorting unit, injection portion still have the shaping in the ring bottom surface of drawing the ring centers on the lug at the bottom of injection hole.

The beneficial effects of the utility model reside in that: the injection part is arranged on the extracting ring, so that the sample containing chamber and the sorting containing chamber are directly and vertically communicated through the injection hole, the semen sample can be quickly injected into the sample containing chamber from the injection hole, the operation is time-saving and labor-saving, the whole structure is simple, and the production cost can be reduced.

The utility model discloses a sperm sorting device, which is suitable for sorting a plurality of movable sperms from a sperm sample, and comprises a base and a sorter;

the base comprises a bottom wall and a surrounding wall which extends from the periphery of the bottom wall to the top end and surrounds the axis, and the bottom wall and the surrounding wall are matched to define an accommodating space with an opening;

the separator is fixedly connected with the base and comprises a kit and a permeable membrane; the sleeve is provided with a sleeve positioned in the accommodating space around the axis, and an injection part extending from the bottom end of the sleeve to the direction close to the axis, the injection part is provided with an injection hole penetrating along the axis, and the sleeve is provided with an inner cylindrical surface and an outer cylindrical surface surrounding the inner cylindrical surface; the permeable membrane is fixed at the bottom end of the sleeve and is matched with the injection part and the inner cylinder surface to define a separation chamber with an open top end, and the permeable membrane is provided with a plurality of micropores for the motile sperms to pass through and a through hole corresponding to the injection hole;

the permeable membrane is matched with the bottom wall and the surrounding wall to define a sample chamber for accommodating the semen sample, and the injection hole is vertically communicated with the sample chamber and the separation chamber.

Sperm sorting unit, the sleeve still has the connection interior bobbin face with the bobbin base face of outer bobbin face, penetrating membrane is fixed in telescopic bobbin base face.

Sperm sorting unit, it still is fixed in to lead to the membrane the bottom surface of injection portion.

The beneficial effects of the utility model reside in that: the injection part is arranged at the bottom end of the sleeve, so that the sample containing chamber and the sorting containing chamber are directly and vertically communicated through the injection hole, the semen sample can be quickly injected into the sample containing chamber from the injection hole, the operation is time-saving and labor-saving, the whole structure is simple, and the production cost can be reduced.

Drawings

FIG. 1 is a schematic combined cross-sectional view of the sperm sorting system of U.S. Pat. No. 10,422,737B 2;

FIG. 2 is a perspective view of the sperm cell sorter of the present invention;

FIG. 3 is a partial exploded perspective view of the first embodiment, illustrating that the first embodiment includes a base, a separator and a lid;

FIG. 4 is an exploded perspective view of the first embodiment;

FIG. 5 is a bottom perspective view of the sorter of the first embodiment;

FIG. 6 is a bottom exploded perspective view of the sorter of the first embodiment;

FIG. 7 is a combined cross-sectional view of the first embodiment;

FIG. 8 is a schematic use diagram of the first embodiment;

FIG. 9 is a partial exploded perspective view of a sperm cell sorting device according to a second embodiment of the present invention, illustrating that the second embodiment includes a base, a sorter, and a cover;

FIG. 10 is a bottom perspective view of the sorter of the second embodiment;

FIG. 11 is a bottom exploded perspective view of the sorter of the second embodiment;

FIG. 12 is a combined cross-sectional view of the second embodiment;

FIG. 13 is a partial exploded perspective view of a sperm cell sorting apparatus according to a third embodiment of the present invention, illustrating that the third embodiment includes a base, a sorter, and a cover; and

fig. 14 is a combined sectional view of the third embodiment.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and examples.

Before the present invention is described in detail, it should be noted that relative terms used in the following description, such as "top" and "bottom", are used with reference to the orientation shown in the figures and the normal use orientation, and similar elements are denoted by the same reference numerals. In the following description, the "cylinder" and the "ring" are shown as circles in the drawings, but the "cylinder" and the "ring" should not be limited to circles.

Referring to fig. 2 to 4 and 8, the sperm sorting device 21 of the first embodiment of the present invention is adapted to sort a plurality of motile sperm 110 from a semen sample 100, and includes a base 3, a sorter 4 and a cover 5.

Referring to fig. 2 to 4 and 7, the base 3 includes a bottom wall 31, a surrounding wall 32 extending from the periphery of the bottom wall 31 toward the top end and surrounding an axis I, a top wall 33 extending from the top end of the surrounding wall 32 toward a direction away from the axis I, an outer wall 34 extending from the periphery of the top wall 33 toward the bottom end and surrounding the axis I, and a central protrusion 35 extending from the central portion of the bottom wall 31 toward the top end along the axis I and having a gradually decreasing diameter (i.e., an outer diameter), wherein the bottom wall 31 and the surrounding wall 32 cooperate to define an accommodating space 36 having an opening 361.

The central protrusion 35 is a truncated cone with a narrow top and a wide bottom, and has a truncated conical surface 351, and a plurality of spaced ribs 352 equidistantly disposed on the truncated conical surface 351.

In the embodiment, the surrounding wall 32 extends from the bottom wall 31 along the axis I toward the top end and has an increasing diameter (i.e., outer diameter), and the accommodating space 36 is a truncated cone-shaped space with a wide top and a narrow bottom.

Referring to fig. 3-8, the sorter 4 is attached to the base 3 and includes a sleeve 41 and a permeable membrane 49.

The separator 4 and the base 3 may be formed integrally or may be formed as two separate members and integrally bonded to each other.

The sleeve 41 has a sleeve 42 surrounding the axis I for being placed in the accommodating space 36 and fixedly connected to the surrounding wall 32, a convex ring 43 extending from the top end of the sleeve 42 in a direction away from the axis I and fixedly connected to the top wall 33 of the base 3, a dip ring 44 extending from the bottom end of the sleeve 42 in a direction close to the axis I, an injection portion 45 disposed on the dip ring 44 and extending along the axis I, and a liquid-taking device-limiting groove 46 disposed on the dip ring 44 and having an open top end.

The sleeve 42 has an inner cylindrical surface 421, an outer cylindrical surface 422 surrounding the inner cylindrical surface 421, and a cylindrical bottom surface 423 connecting the inner cylindrical surface 421 and the outer cylindrical surface 422.

The pumping ring 44 has a top ring surface 441, a bottom ring surface 442, and a central hole 443 connecting the top ring surface 441 and the bottom ring surface 442, and the bottom ring surface 442 extends from the bottom ring surface 423 of the sleeve 42 toward the direction close to the axis I while gradually approaching the top ring surface 441, so that the distance between the bottom ring surface 442 of the pumping ring 44 and the top ring surface 441 gradually decreases from the bottom end of the sleeve 42 to the direction close to the axis I.

The bottom surface 442 of the extraction ring 44 has an outer extension 444 extending from the bottom surface 423 of the sleeve 42 toward the direction close to the axis I, a connection 445 extending from the outer extension 444 toward the direction close to the top surface 441 of the ring and having a decreasing diameter (i.e., outer diameter), and an inner extension 446 extending from the connection 445 toward the direction close to the axis I. The central bore 443 communicates between the ring top surface 441 and the inner extension section 446 of the ring bottom surface 442.

The injection portion 45 connects the connecting section 445 of the ring top surface 441 and the ring bottom surface 442, and has an injection hole 451 extending therethrough along the axis I, a top protrusion 452 formed on the ring top surface 441 of the dip ring 44 and surrounding the injection hole 451, and a bottom protrusion 453 formed on the connecting section 445 of the ring bottom surface 442 of the dip ring 44 and surrounding the injection hole 451.

The liquid-extracting-device-limiting groove 46 extends from the ring top surface 441 toward the ring bottom surface 442, and has a groove bottom 461 and a communicating portion 462 communicating the groove bottom 461 with the central hole 443, and the width of the liquid-extracting-device-limiting groove 46 is 4 μm to 5mm.

The permeable membrane 49 is fixed to the sleeve 42 and cooperates with the extracting ring 44 and the inner cylinder surface 421 to define a separation chamber 6 with an open top end, and the permeable membrane 49 has a plurality of micropores 491 for the passage of the motile sperm 110.

The permeable membrane 49 of the first embodiment is secured to the outwardly extending section 444 of the ring bottom surface 442 of the extraction ring 44 and has a through hole 492 corresponding to the injection hole 451.

The transparent film 49 is further fixed to the bottom surface of the bottom protrusion 453 and the bottom 461 of the liquid-extracting-device limiting groove 46, so that the fixing area between the transparent film 49 and the sleeve 42 can be increased, and the fixing effect of the transparent film 49 can be improved.

The through film 49 is located at the bottom side of the central hole 443, and the liquid-extracting device limiting groove 46 is communicated with the central hole 443 and the through film 49 through the communicating part 462.

When the separator 4 is combined with the base 3, the sleeve 42 of the kit 41 is located in the accommodating space 36, the through film 49 cooperates with the bottom wall 31 and the surrounding wall 32 to define a sample chamber 7 for accommodating the semen sample 100, and the sample chamber 7 and the separation chamber 6 are directly and vertically communicated through the injection hole 451 and the through hole 492.

The top cover 5 includes a cover body 51, and a cover surrounding body 52 extending from the periphery of the cover body 51 toward the bottom end and surrounding the axis I and located on the convex ring 43 and the top wall 33, and the separation chamber 6 can be closed by the top cover 5.

Referring to fig. 4, 7 and 8, in use, firstly, an operator holds a syringe (not shown) containing the semen sample 100 to directly inject the semen sample 100 into the sample chamber 7 through the injection hole 451, then, a sorting solution 120 is dropped on the permeable membrane 49, the sorting solution 120 partially permeates into the sample chamber 7 to form a liquid continuous surface, the sorting solution 120 is any diluent suitable for sperm treatment, then, the standing is performed for 3 to 60 minutes, during the standing process, the motile sperm 110 with strong motility in the semen sample 100 can upwards dissociate to pass through the micropores 491 of the permeable membrane 49 and enter the sorting chamber 6 to mix with the sorting solution 120, finally, the operator inserts a needle of a liquid extractor (not shown, such as a syringe) into the liquid extracting groove 46 and abuts against the bottom 461, so that the sorting solution 120 and the motile sperm 110 with strong motility can be extracted, thereby, the needle of the liquid extractor can be prevented from piercing the permeable membrane 49, and the residual amount of the liquid extractor 442 can be reduced because the permeable membrane 46 is in communication with the bottom 462 of the liquid extractor, and the sorting solution 49 can be extracted through the bottom 462.

It should be noted that, when the separation liquid 120 and the motile sperm 110 are sucked, the spacer ribs 352 of the central protrusion 35 can prevent the semen sample 100 from flowing smoothly, so as to prevent the impurities in the semen sample 100 from being sucked through the transparent film 49, and the impurities can be blocked at the boundary between the truncated conical surface 351 and the spacer ribs 352, so as to prevent the impurities from being sucked through the transparent film 49, thereby improving the separation quality.

Referring to fig. 9 to 12, a sperm sorting device 22 according to a second embodiment of the present invention comprises the base 3, a sorter 4' and the top cover 5.

The separator 4' also includes a sleeve 41' and a permeable membrane 49'.

The kit 41 'also has a sleeve 42', a collar 43', a scooping ring 44', an injection portion 45', and a pipette retaining groove 46'. The extraction ring 44 'also has a ring top surface 441', a ring bottom surface 442', and a central bore 443', and the ring bottom surface 442 'also has an outer extension 444', a connection 445', and an inner extension 446'.

The sperm sorting apparatus 22 is different from the sperm sorting apparatus 21 in that the injection hole 451 'of the injection part 45' penetrates through the extended section 444 'communicating the ring top surface 441' with the ring bottom surface 442', and the permeable membrane 49' is fixed to the extended section 444 'and positioned inside the injection hole 451', so that the permeable membrane 49 'does not need to be perforated corresponding to the injection hole 451'.

The sperm sorting device 22 also defines the sample chamber 7 and the sorting chamber 6, and the sample chamber 7 and the sorting chamber 6 are directly and vertically communicated through the injection hole 451'.

The sperm sorting device 22 and the sperm sorting device 21 have the same operation instructions and can obtain the same effects, and the description thereof is omitted.

Referring to fig. 13 to 14, a sperm sorting device 23 according to a third embodiment of the present invention comprises the base 3, a sorter 4 ″ and the top cover 5.

The separator 4 "also comprises a sleeve 41" and a permeable membrane 49".

The sleeve 41 "has a sleeve 42" surrounding the axis I for being placed in the accommodating space 36 and being fixed to the surrounding wall 32 ", a protruding ring 43 extending from the top end of the sleeve 42" in a direction away from the axis I and being fixed to the top wall 33 of the base 3", and an injection portion 45" extending from the bottom end of the sleeve 42 "in a direction close to the axis I.

The sleeve 42 "also has an inner cylindrical surface 421", an outer cylindrical surface 422", and a cylindrical bottom surface 423".

The injection portion 45 "has an injection hole 451" extending therethrough along the axis I.

The permeable membrane 49 "is fixed to the bottom surface 423" of the sleeve 42 "or the bottom end of the sleeve 42" and has a plurality of micro holes 491 "and a through hole 492" corresponding to the injection hole 451".

Preferably, the transparent film 49 "is further fixed to the bottom surface of the injection part 45", so that the fixing area of the transparent film 49 "can be increased to improve the fixing effect.

The difference between the sperm sorting device 23 and the sperm sorting device 21 is that the drawing ring 44 and the liquid extractor limiting groove 46 (see fig. 4) are omitted from the kit 41", and the transparent membrane 49" is fixed on the bottom surface 423 "of the sleeve 42" and cooperates with the injection part 45 "and the inner cylindrical surface 421" to define a sorting chamber 6 "with an open top end.

The sperm sorting device 23 also defines the sample chamber 7, and the sample chamber 7 and the sorting chamber 6 "are directly and vertically communicated through the injection hole 451".

To sum up, the utility model has the following functions and advantages, thereby really achieving the purpose of the utility model.

1. The utility model discloses setting up this injection hole 451 in the injection portion 45 of this dip ring 44, setting up this injection hole 451 'in the injection portion 45' of this dip ring 44', making this kind of body appearance room 7 and this select separately and hold the direct perpendicular intercommunication of room 6, borrow this can pour into this kind of body appearance room 7 into from this injection hole 451, 451' into this kind of body with this semen examination body 100 fast in, the operation is more time-saving and laborsaving.

2. The utility model discloses setting up this injection hole 451 in this injection portion 45 "of this sleeve 42" bottom, making this appearance room 7 of examining and should select separately and hold room 6 "direct perpendicular intercommunication, borrow this semen examination body can pour into this examination body from this injection hole 451" into this appearance room 7 of examining fast, operate more time-saving and laborsaving.

3. The utility model discloses need not set up the circulation route 15 of current sperm sorting system 10, overall structure is succinct, reduction in production cost.

4. The utility model discloses the circulation route 15 that transversely sets up that need not set up current sperm sorting system 10, consequently the utility model discloses holistic radial dimension need not increase thereupon.

Claims (10)

1. A sperm sorting device is suitable for sorting a plurality of motile sperms from a semen sample, and comprises a base and a sorter; the method is characterized in that:

the base comprises a bottom wall and a surrounding wall which extends from the periphery of the bottom wall to the top end and surrounds the axis, and the bottom wall and the surrounding wall are matched to define an accommodating space with an opening;

the separator is fixedly connected with the base and comprises a sleeve and a permeable membrane,

the sleeve is provided with a sleeve positioned in the accommodating space around the axis, an extracting ring extending from the bottom end of the sleeve to the direction close to the axis, and an injection part arranged on the extracting ring, wherein the injection part is provided with an injection hole penetrating along the axis, and the sleeve is provided with an inner cylindrical surface and an outer cylindrical surface surrounding the inner cylindrical surface;

the permeable membrane is fixed on the sleeve and is matched with the suction ring and the inner cylinder surface to define a separation chamber with an open top end, and the permeable membrane is provided with a plurality of micropores for the motile sperms to pass through;

the penetrating film, the bottom wall and the surrounding wall are matched to define a sample containing chamber for containing the semen sample, and the injection hole is vertically communicated with the sample containing chamber and the sorting containing chamber.

2. The sperm sorting device of claim 1, wherein: the base and the sorter are of an integrated structure.

3. The sperm sorting device of claim 1, wherein: the sleeve is further provided with a cylinder bottom surface connecting the inner cylinder surface and the outer cylinder surface, the pumping ring is provided with a ring top surface, a ring bottom surface and a central hole communicating the ring top surface and the ring bottom surface, and the ring bottom surface of the pumping ring extends from the cylinder bottom surface of the sleeve towards the direction close to the axis and is gradually close to the ring top surface.

4. A sperm sorting device as described in claim 3 wherein: the bottom surface of the sucking ring is provided with an outer extension section extending from the bottom surface of the sleeve towards the direction close to the axis, a connection section extending from the outer extension section towards the direction close to the top surface of the ring, and an inner extension section extending from the connection section towards the direction close to the axis, the central hole is communicated with the top surface of the ring and the inner extension section of the bottom surface of the ring, the injection hole penetrates through the connection section of the top surface of the ring and the bottom surface of the ring, and the transparent film is fixed on the outer extension section and is provided with a through hole corresponding to the injection hole.

5. The sperm sorting device of claim 3, wherein: the bottom surface of the sucking ring is provided with an outer extension section extending from the bottom surface of the sleeve towards the direction close to the axis, a connecting section extending from the outer extension section towards the direction close to the top surface of the ring, and an inner extension section extending from the connecting section towards the direction close to the axis, the central hole is communicated with the top surface of the ring and the inner extension section of the bottom surface of the ring, the injection hole penetrates through the outer extension sections of the top surface and the bottom surface of the ring, and the permeable membrane is fixed on the outer extension section and is positioned on the inner side of the injection hole.

6. The sperm sorting device of claim 3, wherein: the injection part is also provided with a top lug which is formed on the ring top surface of the drawing ring and surrounds the injection hole.

7. The sperm sorting device of claim 6, wherein: the injection part is also provided with a bottom bump which is formed on the ring bottom surface of the sucking ring and surrounds the injection hole.

8. A sperm sorting device is suitable for sorting a plurality of motile sperms from a semen sample, and comprises a base and a sorter; the method is characterized in that:

the base comprises a bottom wall and a surrounding wall which extends from the periphery of the bottom wall to the top end and surrounds the axis, and the bottom wall and the surrounding wall are matched to define an accommodating space with an opening;

the separator is fixedly connected with the base and comprises a sleeve and a permeable membrane,

the sleeve is provided with a sleeve positioned in the accommodating space around the axis and an injection part extending from the bottom end of the sleeve to the direction close to the axis, the injection part is provided with an injection hole penetrating along the axis, and the sleeve is provided with an inner cylindrical surface and an outer cylindrical surface surrounding the inner cylindrical surface;

the permeable membrane is fixed at the bottom end of the sleeve and is matched with the injection part and the inner cylinder surface to define a separation chamber with an open top end, and the permeable membrane is provided with a plurality of micropores for the motile sperms to pass through and a through hole corresponding to the injection hole;

the penetrating film, the bottom wall and the surrounding wall are matched to define a sample containing chamber for containing the semen sample, and the injection hole is vertically communicated with the sample containing chamber and the sorting containing chamber.

9. The sperm sorting device of claim 8, wherein: the sleeve is further provided with a barrel bottom surface for connecting the inner barrel surface and the outer barrel surface, and the permeable membrane is fixed on the barrel bottom surface of the sleeve.

10. The sperm sorting device of claim 9, wherein: the permeable membrane is also fixed to the bottom surface of the injection part.

Images