CN218512312U

CN218512312U - Hand-held type multi-wavelength minute level microorganism detection device in gas

Info

Publication number: CN218512312U
Application number: CN202221464669.2U
Authority: CN
Inventors: 迟海鹏; 张京军
Original assignee: Beijing Dyna Aurora Technology Co ltd
Current assignee: Beijing Dyna Aurora Technology Co ltd
Priority date: 2022-06-13
Filing date: 2022-06-13
Publication date: 2023-02-21
Anticipated expiration: 2032-06-13

Abstract

The utility model discloses a microbial detection device in gaseous of hand-held type multi-wavelength minute level, including multi-wavelength laser raman detection module, enrichment module, high-voltage electric field module, air pump, lotus electric module, airflow channel and automatically controlled module. The gas flow channel is used for conveying gas to be detected to a detection area, the charging module is arranged in a transmission path of the gas flow channel and charges microorganisms in a target sample through high-voltage discharge, the enrichment module is arranged at the downstream of the charging module, the high-voltage electric field module comprises an anode and a cathode, the enrichment module is arranged between the anode and the cathode, and the multi-wavelength laser Raman detection module performs Raman detection towards the enrichment module at a specific angle.

Description

Handheld multi-wavelength minute-level detection device for microorganisms in gas

Technical Field

The utility model discloses microorganism detection fields such as virus in the gas especially relates to a minute level, high accuracy, high sensitivity's detection device that is arranged in the indoor environment or microorganism such as target crowd's virus.

Background

Various microorganisms such as viruses, including influenza virus, SARS virus, and neocoronavirus (COVID-19) are present in the air. The detection and early warning of microorganisms such as viruses in indoor ambient air are very necessary.

The PCR technology is the most common method for screening the microorganisms such as the new coronavirus and the like at present, but the PCR technology has the defects of limited use environment, long detection time, low accuracy rate and the like, and cannot be applied to detection of the microorganisms such as the viruses and the like in an indoor air environment.

SUMMERY OF THE UTILITY MODEL

The utility model discloses an overcome prior art not enough, provide a measuring sensitivity, precision height, the measurement process is quick, and environmental suitability's microorganism detection device such as virus in the multi-wavelength minute level gas can be used for the microorganism condition such as virus in the real-time detection indoor environment, also can be used for the customs to go into the time short-term test entry personnel whether infect the primary screen means of target microorganism such as new canopy.

Particularly, the utility model provides a little biological detection device in hand-held type multi-wavelength minute level gas, including multi-wavelength laser raman detection module, enrichment module, high-voltage electric field module, lotus electric module, airflow channel and automatically controlled module.

The gas flow channel is used for conveying gas to be detected to a detection area, the charging module is arranged in a transmission path of the gas flow channel and charges microorganisms in a target sample through high-voltage discharge, the enrichment module is arranged at the downstream of the charging module, the high-voltage electric field module comprises an anode and a cathode, the enrichment module is arranged between the anode and the cathode, and the multi-wavelength laser Raman detection module performs Raman detection towards the enrichment module at a specific angle.

Preferably, the microorganism detection device further comprises a temperature control module, wherein the temperature control module is arranged below the enrichment module and used for cooling the enrichment module.

Preferably, the laser detection wavelength of the multi-wavelength laser Raman detection module comprises at least one of 532nm,633nm,783nm,830nm and 1064 nm; the emission window is placed at a specific angle to the enrichment module.

Preferably, the microorganism detection device further comprises a power supply module and a replaceable gas collecting port, the power supply module is used for respectively supplying power to the multi-wavelength laser raman detection module, the enrichment module, the high-voltage electric field module, the gas pump and the charging module, and the replaceable gas collecting port is communicated with one end of the gas flow channel and is used for receiving external sample gas.

Preferably, the enrichment module is composed of a metal nano material, preferably a gold nano material with a rough surface, and is used for adsorbing the microbial aerosol such as viruses in the gas.

Preferably, the cathode of the high-voltage electric field module is in a parabolic structure, the inner paraboloid of the cathode faces the enrichment module, the anode of the high-voltage electric field module is in a flat plate shape, and the anode is arranged below the enrichment module.

Preferably, the microorganism detection device further comprises an air pump arranged at the side part of the air flow channel and used for flushing the inside of the device once before a single detection and after the detection is finished.

The charging module comprises one or more pairs of point discharge mechanisms, and applies electric charge to a sample to be tested through point discharge, preferably a high-purity tungsten wire with a specific diameter.

Preferably, one section of the air flow channel is connected with the replaceable air collecting port and the air pump, the middle part of the air flow channel is provided with the buffer air chamber, and the tail end of the air flow channel extends into the high-voltage electric field module after being partially bundled; the tail end is divided into a plurality of gas outlets which are uniformly distributed on the circumference; the other section of the airflow channel is arranged below the enrichment module, the sectional area of the head end is larger than that of the enrichment module, and the tail end extends out of the device.

Preferably, the electric control module comprises a display screen and a control chip, is arranged at the upper end of the device, is connected with the multi-wavelength laser raman detection module, the high-voltage electric field module, the air pump, the charging module, the temperature control module, the electric control module and the power supply module, and is used for analyzing and controlling the working state of the connecting assembly of the electric control module and the electric control module, controlling the detection flow and displaying the detection result on the display screen.

The working process of the device is as follows: firstly, the air pump flushes the interior of the device with a large-flow clean air flow to ensure the interior of the device to be clean, then a target to be detected blows or breathes towards the replaceable gas collecting port, a sample to be detected enters the interior of the device through an air flow channel and is applied with charges by a charging module, microbial aerosol such as viruses in the sample to be detected is efficiently adsorbed on an enrichment module under the action of a high-voltage electric field module, and other samples to be detected are discharged out of the device through another section of air flow channel; then, the multi-wavelength laser Raman detection module emits detection laser with at least one wavelength of 532nm,633nm,783nm,830nm and 1064nm from a specified angle to the detection sample of the enrichment module in a time sequence manner to generate Raman scattering, detects Raman spectrum signals emitted from the enrichment module, and sends the Raman spectrum signals to the electronic control module; the electric control module analyzes the detection result and displays the detection result on the display screen; harmful viruses and other microorganisms are weakly detected, strong voltage is applied to the high-voltage electric field module, and the viruses and other microorganism aerosols on the acquisition module are killed; after the detection is finished, the air pump flushes the interior of the device with large-flow clean air flow.

Compared with the prior art, the utility model discloses the beneficial effect who has does:

1. the utility model discloses a microbial detection device such as virus in hand-held type multi-wavelength minute level gas, its measuring sensitivity, precision are high, have realized minute level and have measured, and have changed the current situation that does not have the actual application at present in the indoor environment and microbial real-time detection methods and devices such as target crowd virus.

2. The utility model discloses the device is through adopting multi-wavelength laser raman to survey the module, and identification structure similar microorganism that can higher accuracy.

3. The utility model discloses an adopt the high velocity of flow air current to wash away the technique and accomplish the washing of killing of enrichment module, and the design can be replaced to the enrichment module, has improved detection device's life, has reduced the detection cost.

Drawings

FIG. 1 is a schematic view of a microorganism detection apparatus according to an embodiment of the present invention;

in the drawings: 1 is a multi-wavelength laser Raman detection module; 2 is an enrichment module; 3 is a high-voltage electric field module; 4 is an air pump; 5 is a charge module; 6 is a replaceable air collecting port; 7 is an airflow channel; 8, a temperature control module (for cooling the whole 2); 9 is an electric control module; and 10 is a power supply module.

Detailed Description

The present invention is described in detail below with reference to the drawings and the embodiments thereof, but the scope of the present invention is not limited thereto.

Example 1:

as shown in fig. 1, the handheld detection device in this embodiment includes a multi-wavelength laser raman detection module 1; an enrichment module 2; a high-voltage electric field module 3; an air pump 4; a charge module 5; a replaceable air collecting port 6; an air flow channel 7; a temperature control module 8; an electronic control module 9; a power supply module 10.

The replaceable gas collection port 6 is an inlet for the sample to be tested, for receiving external sample gas. The inner side of the replaceable air collecting opening 6 is connected with an air flow channel 7, and the air flow channel 7 transmits the sample to be detected entering from the replaceable air collecting opening 6.

The air pump 4 is arranged at the side part of the airflow channel and used for performing primary flushing before single detection and after detection is completed, namely, the inside of the large-flow-rate airflow flushing device is flushed to remove foreign matters attached to the inner wall of the airflow channel 7, the surface of the enrichment module 2 and the surface of the multi-wavelength laser Raman detection module 1, so that the detection accuracy is improved; the air pump 4 can also collect samples at a low flow rate and enter the airflow channel 7 inside the device to detect microorganisms such as viruses in the gas environment when the device is used for monitoring the microorganisms such as viruses in the gas environment.

One section of the air flow channel is connected with the replaceable air collecting port and the air pump, the middle part of the air flow channel is provided with a buffer air chamber, and the tail end of the air flow channel is divided into 8 air outlets which are equidistantly arranged on the circumference and then extends into the high-voltage electric field module; the other section of the airflow channel is arranged below the enrichment module, the sectional area of the head end of the airflow channel is larger than that of the enrichment module, and the tail end of the airflow channel extends out of the device.

The charging module 5 is an electric field applying assembly consisting of two electrodes including one or more pairs of point discharge mechanisms for applying electric charges to the sample to be measured by point discharge, preferably a high purity tungsten wire of a specific diameter, such as 40 microns. The charging module 5 adopts high-voltage discharge, the discharge distance of the tungsten filament is set to be 5-9mm, the charging voltage is +2-5kV, and aerosol charging is carried out on microorganisms passing through the charging module.

The multi-wavelength laser raman detection module 1 emits detection laser light of at least one wavelength of 532nm,633nm,783nm,830nm and 1064nm onto the detection sample of the enrichment module 2 in a time-series manner from a specified angle (for example, inclined by a small angle), generates raman scattering, and detects a raman spectrum signal emitted from the enrichment module 2; the laser detection effects of different wavelengths are different, for example, 532nm laser is adopted, so that the detection sensitivity is higher, but a serious fluorescence effect exists; for another example, the detection resolution is high by using 783nm laser; by adopting the scheme of time sequence and multiple wavelengths, the accuracy and the sensitivity of the device for detecting microorganisms such as different types of viruses and the like can be improved, and the device has better effects on distinguishing active viruses from inactivated viruses and distinguishing viruses with similar structures, such as different coronaviruses.

The enrichment module 2 is made of metal nano materials, preferably gold nano materials with rough surfaces and used for adsorbing microbial aerosol such as viruses in gas; the surface nano configuration can generate a stronger local electric field and enhance the Raman intensity. The sparsity of the gold nano-material in the enrichment module 2 is sufficient for the laser to pass through. The enrichment module adopts a surface nano configuration to generate a stronger local electric field and enhance the strength of a Raman effect.

The high-voltage electric field module 3 consists of a positive electrode and a negative electrode, the voltage is adjustable, and the high-voltage electric field which points and concentrates on the surface of the enrichment module 2 is applied to the enrichment module 2 to weakly ionize the ambient air, so that free charges are attached to the nano surface, the plasma density of the surface of the nano material can be greatly improved, the Raman intensity is enhanced, and the detection sensitivity is greatly improved; can also be used for killing microorganisms such as viruses and the like under the condition of applying specific high voltage; the electrodes are preferably made of red copper, one of the electrodes (preferably the cathode) is parabolic, and the other electrode is flat, so that the electric field converges towards the enrichment module 2. In this embodiment, as shown in the figure, the anode is a parabolic cover above the enrichment module 2, and the anode is a flat plate shape and is disposed below the enrichment module 2, although the cathode and the anode can be interchanged according to the use requirement.

The high-voltage electric field module consists of positive and negative electrodes positioned on two sides of the enrichment module, and the voltage is adjustable, so that the collection efficiency of the enrichment module on the aerosol of the microorganisms such as viruses is improved, and the high-voltage electric field module can also be used for killing the microorganisms such as viruses under the condition of applying a specific voltage; the electrode is preferably made of red copper and is in a paraboloid shape, so that the electric field is converged towards the enrichment module.

The irradiation of the Raman laser can form a strong electric field on the surface of the enrichment module 2, and the high-voltage electric field module 3 further applies the strong electric field to the enrichment module 2, so that the number of free electrons contained in the enrichment module 2 is greatly increased, and Raman spectrum information of the virus and other microbial aerosols is obtained by utilizing a Raman detection assembly through a forward multi-pass surface enhanced Raman effect principle. The airflow pipeline penetrates through the hole reserved on the arc electrode and extends into the space between the arc electrode and the flat electrode, gas is conveyed to the enrichment module 2, the effect of the electric field on charged microorganisms is matched, the microorganisms with more charges further move towards the enrichment module 2 under the effect of the electric field force, and the optimal enrichment effect is guaranteed. And other particulate matters such as PM2.5, PM10 and the like have little charge and little applied force of an electric field, and are blown out of the gating enrichment module 2 under the action of the airflow.

Because a strong electric field is applied to the enrichment module 2 through the high-voltage electric field module, the number of free electrons contained in the enrichment module 2 is increased by at least one magnitude, so that the electric field intensity of the surface of the metal nano structure is increased by at least 10 times, after the microorganisms are adsorbed to the enrichment module 2, laser emitted by a laser source of the Raman module is filtered and finally irradiated onto the microorganisms adsorbed in the enrichment module 2, and then corresponding Raman signals are collected to determine the components of the microorganisms.

The temperature control module 8 is arranged below the enrichment module 2 and above the cathode of the high-voltage electric field module 3 and is attached to the enrichment module 2, the temperature of gas in the high-voltage electric field cavity can be detected by using a temperature sensor, the temperature of the enrichment module 2 is controlled, the temperature difference between the temperature of the enrichment module 2 and the temperature of the gas in the high-voltage electric field cavity is a set value, aerosol such as viruses can be condensed on the enrichment module 2 by adopting the mode, the collection efficiency is improved, and the preferred temperature control module 8 is a semiconductor temperature control module 8.

The electric control module 9 comprises a display screen and a control chip, is arranged at the upper end of the device, is connected with the multi-wavelength laser Raman detection module 1, the high-voltage electric field module 3, the air pump 4, the charging module 5, the temperature control module 8, the electric control module 9 and the power supply module 10, and is used for analyzing and controlling the working state of the connecting assembly thereof, controlling the detection flow and displaying the detection result on the display screen.

The power supply module 10 is composed of a rechargeable battery and an inverter, is arranged at the bottom of the device, is connected with the multi-wavelength laser Raman detection module 1, the high-voltage electric field module 3, the air pump 4, the charge module 5, the temperature control module 8 and the electric control module 9, and is used for supplying power to each component connected with the power supply module. Of course, the handheld device can be powered by an external power supply without a power supply module arranged in the handheld device.

The utility model discloses the work flow of device does: firstly, the air pump 4 uses a large-flow clean air flow to wash the inside of the device to ensure the inside of the device to be clean, then a target to be detected blows or breaths towards the inside of the replaceable air collecting port 6 (or directly collects the ambient air of a target area, and sends a sample into the device through an additional air pump without blowing or breathing of the target), the sample to be detected enters the inside of the device through the air flow channel 7 and is applied with electric charges by the charge module 5, microbial aerosol such as viruses and the like in the sample to be detected is efficiently adsorbed on the enrichment module 2 under the action of the high-voltage electric field module 3, and other samples to be detected are discharged out of the device through the other air flow channel 7; then, the multi-wavelength laser raman detection module emits detection laser with at least one wavelength (preferably multiple emission in turn) of 532nm,633nm,783nm,830nm and 1064nm from a specified angle in a time sequence manner onto the detection sample of the enrichment module 2 to generate raman scattering, detects a raman spectrum signal emitted from the enrichment module 2, and sends the raman spectrum signal to the electronic control module 9; the electric control module 9 analyzes the detection result and displays the detection result on the display screen; if harmful viruses and other microorganisms are detected, the high-voltage electric field module 3 automatically applies strong voltage to kill the viruses and other microorganisms on the acquisition module; after the detection is finished, the air pump 4 flushes the interior of the device by using a large-flow clean air flow.

The utility model discloses the device collection detects, disappears and kills in an organic whole, and is small, and it is low with use cost to make, and it is comprehensive various to use the scene, is applicable to the quick high accuracy detection of microorganism such as virus in the gas, if the preliminary screening of microorganism such as virus in crowd's exhalate breath on a large scale and the detection of microorganism such as virus in the indoor gas environment and so on.

In this embodiment, the processor employs an artificial intelligence pattern analysis processing technique, including spectrum preprocessing, and then uses a principal component analysis method and an artificial intelligence pattern recognition method supervised by an SVM support vector machine to obtain unique structural models of microorganisms such as different viruses. During detection, the collected graph is compared with the structural model library, so that microorganisms such as different viruses can be matched and identified.

Example 2

In another preferred implementation, the upper surface of the plate electrode has a discharge tip disposed near a focus of the detection light of the raman spectrum detection module. The position of the discharge tip of the anode module can be adjusted according to requirements, and is preferably positioned near the focus of laser emitted by the Raman spectrum detection module. The Raman detection module can move in coordination with the discharge tip of the positive module, so that the discharge tip is continuously located near the focus of the Raman spectrum module, and the position of the discharge tip on the sample collection module can be continuously changed.

And lightly contacting with the sample to be detected; arranging the negative electrode module around the sample collection module and slightly contacting with a sample to be detected; the power supply module is turned on, the current (uA to mA magnitude) is adjusted according to the tolerance degree of different samples to the current to ensure that the structure of the substance to be detected is not damaged, and the substance to be detected in the sample to be detected is charged under the action of the micro-current and is gathered to the vicinity of the discharge tip of the positive electrode module along with the current flow direction; meanwhile, the power module can be connected with another group of electrodes, the group of electrodes are arranged above and below (if the electrodes are arranged on the upper side and the lower side, a grid electrode mode can be adopted to avoid blocking laser) or on the left side and the right side of the sample collection module, and an electric field is applied to the sample collection module, so that free electrons contained in the nano gold material in the sample collection module are greatly increased; the Raman spectrum detection module emits laser, the focus of the laser irradiates the sample collection module, the returned Raman spectrum signal of the substance to be detected is collected at the same time, and the Raman spectrum signal is received and processed by a built-in spectrometer to obtain fingerprint spectrum information of the substance to be detected, so that the detection is completed.

While the principles of the invention have been described in detail in connection with the preferred embodiments thereof, it will be understood by those skilled in the art that the foregoing embodiments are merely illustrative of exemplary implementations of the invention and are not to be construed as limiting the scope of the invention. The details in the embodiments do not constitute the limitations of the scope of the present invention, and any obvious changes such as equivalent transformation, simple replacement, etc. based on the technical solution of the present invention all fall within the protection scope of the present invention without departing from the spirit and scope of the present invention.

Claims

1. A hand-held multi-wavelength minute-level detection device for microorganisms in gas comprises a multi-wavelength laser Raman detection module, an enrichment module, a high-voltage electric field module, a charge module, an airflow channel and an electric control module,

2. The microbial detection apparatus of claim 1, further comprising a temperature control module disposed below the enrichment module for cooling the enrichment module.

3. The microorganism detection apparatus according to claim 1, wherein the laser detection wavelengths of the multi-wavelength laser raman detection module include at least one of 532nm,633nm,783nm,830nm and 1064 nm; the emission window is placed at a specific angle to the enrichment module.

4. The microorganism detection device according to claim 1, further comprising a power supply module and a replaceable gas collection port, wherein the power supply module is used for respectively supplying power to the multi-wavelength laser Raman detection module, the enrichment module, the high-voltage electric field module, the gas pump and the charging module, and the replaceable gas collection port is communicated with one end of the gas flow channel and is used for receiving external sample gas.

5. The microorganism detection apparatus according to claim 1, wherein the enrichment module is made of metal nano-materials and is used for adsorbing the microorganism aerosol such as virus in the gas.

6. The microorganism detection apparatus according to claim 1, wherein the cathode of the high voltage electric field module has a parabolic configuration with an inner parabolic surface facing the enrichment module, the anode of the high voltage electric field module has a flat plate shape, and the anode is disposed below the enrichment module.

7. The microorganism detection apparatus according to claim 1, further comprising an air pump provided at a side of the air flow path for performing a flushing of the inside of the apparatus each time before a single detection and after completion of the detection.

8. The microorganism detection apparatus according to claim 1, wherein the charging module comprises one or more pairs of point discharge mechanisms for applying an electric charge to the sample to be detected by point discharge.

9. The microorganism detection device according to claim 1, wherein one section of the air flow channel is connected with the replaceable air collecting port and the air pump, wherein a buffer air chamber is arranged in the middle of the air flow channel, and the tail end of the air flow channel partially extends into the high-voltage electric field module; the tail end is divided into a plurality of beams of air outlets which are uniformly distributed on the circumference; the other section of the airflow channel is arranged below the enrichment module, the sectional area of the head end is larger than that of the enrichment module, and the tail end extends out of the device.

10. The microorganism detection apparatus according to claim 1,

the electric control module comprises a display screen and a control chip, is arranged at the upper end of the device, is connected with the multi-wavelength laser Raman detection module, the high-voltage electric field module, the air pump, the charge module, the temperature control module, the electric control module and the power supply module, and is used for analyzing and controlling the working state of the connecting assembly of the electric control module and the electric control module, controlling the detection flow and displaying the detection result on the display screen.