CN218491708U - Multi-buckle optical flat cover - Google Patents
Multi-buckle optical flat cover Download PDFInfo
- Publication number
- CN218491708U CN218491708U CN202222489224.6U CN202222489224U CN218491708U CN 218491708 U CN218491708 U CN 218491708U CN 202222489224 U CN202222489224 U CN 202222489224U CN 218491708 U CN218491708 U CN 218491708U
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- China
- Prior art keywords
- pcr
- flat cover
- reaction tube
- optical flat
- hollow cylindrical
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- 230000003287 optical effect Effects 0.000 title claims description 24
- 238000003752 polymerase chain reaction Methods 0.000 claims description 52
- 238000007789 sealing Methods 0.000 claims description 16
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000002834 transmittance Methods 0.000 claims 1
- 239000000443 aerosol Substances 0.000 abstract description 6
- 238000001704 evaporation Methods 0.000 abstract description 6
- 230000008020 evaporation Effects 0.000 abstract description 6
- 230000006978 adaptation Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 239000003292 glue Substances 0.000 description 3
- 238000001746 injection moulding Methods 0.000 description 3
- 238000012408 PCR amplification Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000002313 adhesive film Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
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Abstract
The utility model discloses a multiple optics flat lid of detaining, including the flat lid body, the flat lid body allies oneself with the big or small adaptation of top plane board of calandria with PCR board or PCR, arrange and the vertical fixation has the hollow cylindricality body with the reaction tube hole one-to-one on PCR board or PCR antithetical couplet calandria on the bottom surface of flat lid body, the periphery of each hollow cylindricality body all is fixed with three protruding ring from top to bottom, and the external diameter of two first protruding rings that lean on down is less than the external diameter of the protruding ring of the second of top, circular draw-in groove has all been seted up to each reaction tube hole entrance on PCR board or PCR antithetical couplet calandria, it is downthehole that hollow cylindricality body can stretch into the reaction tube, and the sealed block of three protruding ring homoenergetic and the circular draw-in groove that corresponds on the hollow cylindricality body. The utility model discloses sealed not tight condition can not appear in low temperature and high temperature environment yet, can not cause "swell", "sunken", "sealed not tight", "evaporation weeping", "the appearance of various circumstances such as aerosol pollution".
Description
Technical Field
The utility model relates to a biological consumptive material technical field, more specifically say, relate to a multiple flat lid of optics of detaining.
Background
The PCR plates commonly available on the market comprise 0.2ml half skirt 96-well PCR plates, 0.2ml skirt-free 96-well PCR plates, 0.1ml skirt-free 96-well PCR plates and the like, which are similar in structure, are all 96 wells, and are provided with a flat plate, and the sealing is realized by adopting a plate sealing film manner, such as an adhesive film, a pressure sensitive film, a heat sealing film and the like.
However, the sealing by means of a closure film has the following disadvantages:
1. because the viscosity of the glue film is not enough at low temperature, the glue film is easy to melt at high temperature, and therefore, the glue film can bulge, dent, untight seal, evaporation leakage, aerosol pollution and the like under the environment of low-temperature, high-temperature and high-altitude low-pressure transportation, so that the effect of the fluorescent PCR amplification is influenced.
2. The pressure-sensitive film is easy to shrink and deform at low temperature, and is easy to have poor sealing conditions such as recess and the like at high temperature.
3. Although heat-sealable films perform well at low and high temperatures, the principle of heat-sealable films is that the films and 96-well PCR plates are melted together at high temperatures and are therefore difficult to tear again after cooling.
Therefore, a sealing structure needs to be designed to solve the problem that the fluorescent PCR amplification effect is affected by various conditions such as bulging, sinking, poor sealing, evaporation leakage, aerosol pollution and the like of various sealing plate films in the fluorescent quantitative PCR process in the environments of low temperature, high altitude and low pressure transportation.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides a can be to 96 hole PCR boards, can dismantle 96 hole PCR boards and eight allies oneself with the pipe and seal to can carry out the optical flat lid of detaining repeatedly many times that many times the sealed used, its concrete technical scheme is as follows:
a multi-buckled optical flat cover can be adapted to the top of a PCR plate or a PCR row tube and seal reaction tube holes on the PCR plate or the PCR row tube; the multi-buckled optical flat cover comprises a flat cover body, the flat cover body is matched with a PCR plate or a top plane plate of a PCR (polymerase chain reaction) row tube in size, hollow cylindrical bodies which are in one-to-one correspondence with reaction tube holes on the PCR plate or the PCR row tube are arranged and vertically fixed on the bottom surface of the flat cover body, three convex circular rings are fixed on the periphery of each hollow cylindrical body from top to bottom, the outer diameters of two first convex circular rings close to the bottom are smaller than the outer diameter of the second convex circular ring at the top, a circular clamping groove is formed at the inlet of each reaction tube hole on the PCR plate or the PCR row tube, the hollow cylindrical body can extend into the reaction tube holes, and the three convex circular rings on the hollow cylindrical body can be clamped with the corresponding circular clamping grooves in a sealing manner; the position of the flat cover body corresponding to the hollow cylindrical body is of a high-light-transmission surface plate structure.
Through adopting the technical scheme, the utility model relates to a three protruding ring has been fixed altogether in the periphery of the hollow cylindricality body of bottom in the multiple optics flat lid of detaining, has adopted the design of multiple knot promptly. Therefore, before adding a sample, a worker can cover the multi-buckled optical flat cover, and only one or two raised circular rings below the hollow cylindrical body are firstly clamped with the circular clamping grooves in a sealing manner, so that pre-sealing is realized; after a sample is added, when a worker buckles the multi-buckled optical flat cover again, the three raised circular rings on the hollow cylindrical body are all pressed into the circular clamping grooves directly, and the final complete sealing buckling of the second raised circular rings can be realized due to the fact that the outer diameter of the second raised circular ring at the top is larger than the outer diameters of the two first raised circular rings at the bottom.
The utility model discloses three protruding ring in the flat lid of multiple knot optics can with PCR board or PCR ally oneself with the reaction tube hole on the calandria and closely combine, can not appear sealed tight condition yet in the environment of low temperature (30 degrees below zero) and high temperature (105 degrees), can not cause "swell", "sunken", "sealed not tight", "evaporation weeping", "the appearance of various circumstances such as aerosol pollution".
Preferably, the top circle area of the high light-transmitting surface plate structure is lower than its surrounding area.
Preferably, the top circle area of the high light-transmitting surface plate structure is 0.2-0.3mm lower than the surrounding area.
Preferably, a plurality of openings are uniformly formed in the flat cover body, and the openings and the high-light-transmission surface plate structure do not interfere with each other.
Preferably, the multi-buckled optical flat cover is formed by injection molding of PP plastic.
Preferably, the thickness of the flat cover body is 5 mm-6 mm.
By adopting the technical scheme, the utility model discloses following beneficial effect has:
1. the utility model relates to a multiple optics flat lid of detaining has solved the sealed not tight condition of PCR board closing plate membrane at low temperature and high temperature on the existing market, can not appear "swell moreover", "sunken", "sealed not tight", "evaporation weeping", "aerosol pollutes various circumstances such as".
2, the utility model discloses can store for the reagent, new hat diagnostic reagent's "partial shipment in advance", low temperature transport, high altitude low pressure transportation and molecular laboratory PCR amplify and provide reliable and stable effect more.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the description below are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is an isometric view of a multi-threaded optical flat cover in an embodiment of the present invention.
Fig. 2 is a front view of a multi-fastened optical flat cover according to an embodiment of the present invention.
Fig. 3 is a rear view of an optical multi-buckle flat cover according to an embodiment of the present invention.
Fig. 4 is a side view of an optical flat cover with multiple buckles according to an embodiment of the present invention.
Fig. 5 is an isometric view of a 96-well PCR plate adapted to an embodiment of the invention.
Fig. 6 is an isometric view of a 96-well PCR plate in accordance with an embodiment of the present invention.
FIG. 7 is a front view of the embodiment of the present invention mounted on a 96-well PCR plate.
FIG. 8 is a side view of the embodiment of the present invention mounted on a 96-well PCR plate.
In the figure: 1-flat cover body, 2-hollow cylindrical body, 3-first raised circular ring, 4-second raised circular ring, 5-high light-transmitting surface plate structure, 6-open hole, 7-96 hole PCR plate, 8-top plane plate and 9-reaction tube hole.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
The embodiment is as follows:
the utility model relates to a multiple optics flat lid of detaining can the adaptation install at PCR board or PCR allies oneself with the top of calandria to reaction tube hole 9 on the calandria is allied oneself with to PCR board or PCR seals. As shown in fig. 1 to 8, the multi-snap optical flat cap in this embodiment is a 96-up multi-snap optical flat cap for sealing a 96-well PCR plate 7.
The utility model is characterized in that the transparent PP plastic is manufactured into a specific shape by manufacturing a mould and adopting an injection molding process,
this optical flat lid of multiple knot includes flat lid body 1, the adaptation of 8 sizes in the top plane board of flat lid body 1 and 96 hole PCR board 7, arrange on the bottom surface of flat lid body 1 and the vertical fixation have with the hollow cylindricality body 2 of the 9 one-to-ones of reaction tube hole on the 96 hole PCR board 7, the periphery of each hollow cylindricality body 2 all is fixed with three protruding ring from top to bottom, and the external diameter that leans on two first protruding rings 3 is less than the external diameter of the protruding ring 4 of the second of the top, circular draw-in groove has all been seted up to each reaction tube hole entrance on the 96 hole PCR board 7, hollow cylindricality body 2 can stretch into in the reaction tube hole 9, and the sealed block of three protruding ring homoenergetic and the circular draw-in groove that corresponds on the hollow cylindricality body 2, play sealing effect.
The position of the flat cover body 1 corresponding to the hollow cylindrical body 2 is a high light-transmitting surface plate structure 5, and the structure can improve the optical signal intensity of a reaction system in the PCR reaction tube, so that the detection precision is improved.
Meanwhile, the top of the high-light-transmission surface plate structure 5 adopts a plane-descending technology, namely, the circle area at the top is lower than the surrounding area, and the height is generally 0.2-0.3mm. The purpose of the face is to avoid damage to the high-transparency hole by sharp instruments and other factors.
Furthermore, a plurality of openings 6 are uniformly formed on the flat cover body 1, and the openings 6 are not interfered with the high-light-transmission surface plate structure 5. If flat lid body 1 is a monoblock PP plastics, then arch camber easily, the unevenness, and design trompil 6 back then can guarantee flat lid body 1's level and smooth on flat lid body 1.
Meanwhile, the thickness of the flat lid body 1 is generally 5mm to 6mm.
The utility model discloses form through mould manufacturing process and plastics molding process preparation, the preparation step is:
1. and (3) manufacturing a mould, namely manufacturing the mould for producing the product through a complex process.
And 2, establishing a dust-free workshop, and purchasing an injection molding machine and a plurality of auxiliary machine accessories.
3, production and optimization, and a series of optimization and improvement are carried out through PCR experiments, high-temperature experiments, low-temperature experiments and the like.
In general, only one protrusion is designed on a normal cover during sealing and clamping, which can lead to that the cover is not easy to open after being fastened. And the utility model discloses the flat lid of multiple knot optics then is in the periphery of hollow cylindricality body 2 from top to bottom done three protruding ring, and be one big two little. Because the PCR board or PCR allies oneself with when calandria transportation does not need the heating, so need not cover too tightly, for the convenience of follow-up opening, can only buckle into one or two first protruding ring 3 of lid lower part earlier when leaving the factory can. Thus, after the transportation is finished, the cover can be easily opened to add the sample. After the sample is added, the second convex ring 4 with the larger outer diameter at the upper part is also buckled from top to bottom, so that the whole cover can be completely buckled, and the laboratory pollution caused by liquid leakage in the experimental process is avoided. The simple point is that, the utility model discloses can realize conveniently uncapping midway to and the process of straining the lid again completely at last.
The utility model discloses can guarantee that the low temperature is preserved, the no weeping of low temperature transportation and aviation negative pressure transportation, indeformable, it adopts three protruding ring and PCR board or PCR to ally oneself with the reaction tube hole 9 of calandria closely to combine together after having added the sample, avoided appearing sealed not tight condition in the environment of low temperature (30 degrees below zero) and high temperature (105 degrees), can not cause "swell", "sunken", "sealed not tight", "evaporation weeping", "the appearance of various circumstances such as aerosol pollution", be fit for using widely.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A multi-buckled optical flat cover can be adapted to the top of a PCR plate or a PCR row tube and seal reaction tube holes on the PCR plate or the PCR row tube; the optical flat cover is characterized by comprising a flat cover body, wherein the flat cover body is matched with the size of a PCR plate or a top plane plate of a PCR (polymerase chain reaction) array tube, hollow cylindrical bodies which are in one-to-one correspondence with reaction tube holes on the PCR plate or the PCR array tube are arranged and vertically fixed on the bottom surface of the flat cover body, three convex circular rings are fixed on the periphery of each hollow cylindrical body from top to bottom, the outer diameters of the two adjacent first convex circular rings are smaller than the outer diameter of the uppermost second convex circular ring, a circular clamping groove is formed at the inlet of each reaction tube hole on the PCR plate or the PCR array tube, the hollow cylindrical body can extend into the reaction tube holes, and the three convex circular rings on the hollow cylindrical body can be clamped with the corresponding circular clamping grooves in a sealing manner; the position of the flat cover body corresponding to the hollow cylindrical body is of a high-light-transmission surface plate structure.
2. The multifold optical flat cover according to claim 1, wherein the top circle region of the high light-transmitting surface plate structure is lower than its surrounding region.
3. The multi-button optical flat cover according to claim 2, wherein the top circle region of the high light-transmitting surface plate structure is lower than its surrounding region by 0.2-0.3mm.
4. The multi-fastened optical flat cover according to claim 1, wherein a plurality of openings are uniformly formed on the flat cover body, and the openings do not interfere with the high-transmittance surface plate structure.
5. The multi-snap optical flat cover according to claim 1, wherein said multi-snap optical flat cover is injection molded from PP plastic.
6. The multi-snap optical flat cover according to claim 1, wherein the thickness of the flat cover body is 5mm to 6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222489224.6U CN218491708U (en) | 2022-09-20 | 2022-09-20 | Multi-buckle optical flat cover |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222489224.6U CN218491708U (en) | 2022-09-20 | 2022-09-20 | Multi-buckle optical flat cover |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218491708U true CN218491708U (en) | 2023-02-17 |
Family
ID=85190085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222489224.6U Active CN218491708U (en) | 2022-09-20 | 2022-09-20 | Multi-buckle optical flat cover |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218491708U (en) |
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2022
- 2022-09-20 CN CN202222489224.6U patent/CN218491708U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder |
Address after: 523000 No. 8, South 6th Street, Heshi Road, Qiaotou Town, Dongguan, Guangdong Patentee after: Jihe life science and Technology (Dongguan) Co.,Ltd. Address before: 523000 room 108, building 2, No. 93, Dazhou Daxin Road, Qiaotou town, Dongguan City, Guangdong Province Patentee before: Jihe life science and Technology (Dongguan) Co.,Ltd. |
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| CP02 | Change in the address of a patent holder |