CN218485217U

CN218485217U - Multipurpose chromatographic column

Info

Publication number: CN218485217U
Application number: CN202222809773.7U
Authority: CN
Inventors: 刘容麟; 李岩异; 张卫婷; 陈金利; 张红霞
Original assignee: Ncpc Genetech Biotechnology Development Co ltd
Current assignee: Ncpc Genetech Biotechnology Development Co ltd
Priority date: 2022-10-25
Filing date: 2022-10-25
Publication date: 2023-02-17
Anticipated expiration: 2032-10-25

Abstract

The utility model relates to a multipurpose chromatography column, including the protective sheath be provided with a plurality of chromatography column in the protective sheath, the upper end of all chromatography columns is through last connecting pipe and last minute liquid linker intercommunication, it has last liquid inlet pipe to go up minute liquid linker intercommunication, is provided with the three-way valve on every last connecting pipe, and the lower extreme of all chromatography columns is through connecting pipe and lower minute liquid linker intercommunication down, divide liquid linker intercommunication down to have drain pipe, be provided with the three-way valve under every on the connecting pipe, wherein two there is last drain pipe in the upper end of chromatography column through the three-way valve intercommunication, and the upper and lower both ends of chromatography column form series connection through the three-way valve intercommunication. The utility model discloses can be used for multiple chromatography mode, the chromatography column no longer need whole root chromatography load very high, has reduced the dress post degree of difficulty to the efficiency that the chromatography column was handled has been improved. The utility model discloses required space height is little, and the column casing passageway is closely concentrated, has improved space availability factor.

Description

Multipurpose chromatographic column

Technical Field

The utility model relates to a chromatographic column, in particular to a multipurpose chromatographic column.

Background

Chromatography is an essential purification step in the purification process of biological medicines, and chromatographic columns with different heights are often suitable for using a part of specific media, for example, a chromatographic column with the height of 20cm is suitable for chromatography with low requirements on column efficiency, such as ion, affinity chromatography, hydrophobicity and the like, in an adsorption and desorption mode, but not for size exclusion mode chromatography; the chromatographic column with the height of 70-100cm is suitable for molecular sieve chromatography with higher requirements on column efficiency, but is not suitable for adsorption and desorption type chromatography of ions, affinity and reverse phase. The chromatography of the molecular sieve is a chromatography with high utilization rate of purified target protein in the biomedical industry, but the chromatography usually needs a high-height chromatographic column, and a column filling station is additionally filled at the top of the chromatographic column, so that more inconvenience is brought to the filling of the chromatographic column, the high chromatographic column is easy to dump, and the requirement on the height of space is high. The pretreatment of the chromatographic column requires alkali washing and buffer washing, and the pretreatment needs to be prepared for a long time in advance because the higher back pressure of the chromatographic column is large and the used flow rate is often lower.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a multipurpose chromatographic column to solve current chromatographic column and can't be applicable to different medium, usage single, partial chromatographic column highly higher leads to packing and the inconvenient problem of pretreatment.

The utility model discloses a realize like this: the utility model provides a multipurpose chromatography column, includes the protective sheath be provided with a plurality of chromatography column in the protective sheath, the upper end of all chromatography columns is through last connecting pipe and last minute liquid linker intercommunication, it has last liquid pipe to go up minute liquid linker intercommunication, is provided with the three-way valve on every last connecting pipe, and the lower extreme of all chromatography columns is through connecting pipe and lower minute liquid linker intercommunication down, divide liquid linker intercommunication down to have drain pipe, be provided with the three-way valve under every on the connecting pipe, two of them the upper end of chromatography column has last drain pipe through the three-way valve intercommunication, and the upper and lower both ends of chromatography column form the series connection route through the three-way valve intercommunication.

The chromatographic column comprises a cylinder body, wherein the cylinder body is fixed in the protective sleeve, an upper column head and a lower column head are respectively arranged at the upper end and the lower end of the cylinder body, a column head rod is arranged on the upper column head, a pipeline rod penetrates through the column head rod, the lower end of the pipeline rod is communicated with an inner cavity of the chromatographic column, and the upper end of the pipeline rod is communicated with the upper connecting pipe.

And a column head fixing piece is sleeved on the column head rod and fixed at the upper port of the cylinder body.

And a column head fastener is arranged at the top end of the column head rod and is used for communicating the upper connecting pipe with the pipeline rod.

The chromatography column is four in total, wherein two the upper end of chromatography column has last drain pipe through the three-way valve intercommunication, and the upper end that remains two chromatography columns passes through the three-way valve and communicates each other, and two chromatography column lower extremes that the intercommunication has last drain pipe communicate each other through the three-way valve with one in remaining two chromatography columns respectively.

The utility model discloses be in the same place a plurality of chromatography columns are fixed, and pass through the pipeline intercommunication with a plurality of chromatography columns, realize multiple intercommunication mode through the switch of control three-way valve, if a plurality of chromatography columns use respectively alone or form parallelly connected, or a plurality of chromatography columns establish ties and use to be applicable to different chromatography modes.

For the chromatography in the size exclusion mode, all three-way valves are adjusted to connect all the chromatographic columns in series, so that the use height of the chromatographic columns is improved, and the same effect of the chromatographic column with a single column higher is achieved; by adjusting the upper and lower paths, the chromatographic columns are changed into parallel connection, so that the pretreatment and balance time of the chromatographic columns can be obviously shortened, and the time is saved for the molecular sieve chromatography process.

For the chromatography in the adsorption elution mode, after the chromatographic columns are well balanced by pretreatment, the three-way valve can be adjusted in an optimization stage, and only one passage of the chromatographic column is connected each time, so that the contrast optimization experiment of different conditions of a sample can be more conveniently performed, and after each passage is used, all the passages are opened, and are regenerated and pretreated at the same time, so that the preparation time is shortened, and the time utilization efficiency of a chromatographic system is improved.

The utility model discloses can be used for adsorbing the medium of elution mode, improve the optimization efficiency and the chromatographic column treatment effeciency of process sieve condition, also can be used to the chromatography of size exclusion mode, the chromatographic column no longer need whole root chromatography to load very high, has reduced the dress post degree of difficulty to the efficiency that the chromatographic column was handled has been improved. And simultaneously, the utility model discloses required space height is little, and the column casing passageway is closely concentrated, compares the space availability factor that has improved the chromatography post than the chromatography in the past.

Drawings

Fig. 1 is a structural view of the present invention.

Fig. 2 is a plan view of the present invention.

Fig. 3 is a bottom view of the present invention.

In the figure: 1. a protective sleeve; 2. a chromatography column; 3. a post rod; 4. a pipe line; 5. an upper connecting pipe; 6. an upper liquid separation communicating vessel; 7. an upper liquid inlet pipe; 8. an upper liquid outlet pipe; 9. a three-way valve; 10. a lower connecting pipe; 11. a lower liquid separation communicating vessel; 12. a lower liquid outlet pipe; 13. a stud fastener; 2-1, a cylinder body; 2-2, mounting chapiter; 2-3, lower stigma; 2-4, fixing the column head.

Detailed Description

As shown in fig. 1, fig. 2 and fig. 3, the utility model discloses a protective sheath 1 is provided with a plurality of chromatography column 2 in protective sheath 1, and protective sheath 1 is together fixed with a plurality of chromatography column 2, simultaneously through the outer wall of protective sheath 1 protection chromatography column 2, and whole device is installed and is fixed through protective sheath 1.

Wherein, the chromatographic column 2 comprises a cylinder body 2-1, and the cylinder body 2-1 is penetrated in the through hole of the protective sleeve 1 and is fixed by the protective sleeve 1. An upper column cap 2-2 and a lower column cap 2-3 are respectively arranged at the upper end and the lower end of the cylinder body 2-1, and the upper column cap 2-2 and the lower column cap 2-3 seal the two ends of the cylinder body 2-1 to form a sealed space for containing media. A column head rod 3 is arranged on the upper column head 2-2, a pipeline rod 4 is connected in the column head rod 3 in a penetrating way, the pipeline rod 4 is supported and protected by the column head rod 3 on the outside, and the upper column head 2-2 is moved through the column head rod 3.

The lower end of the pipeline rod 4 is communicated with the inner cavity of the chromatographic column 2, and the upper end of the pipeline rod 4 is communicated with the upper connecting pipe 5, so that liquid can be input into or output from the chromatographic column 2.

A column head fixing piece 2-4 is sleeved on the column head rod 3, and the column head fixing piece 2-4 is fixed at the upper port of the cylinder body 2-1. After the position of the upper column head 2-2 is determined, the column head rod 3 is fixed through the column head fixing part 2-4, and the shaking of the column head rod 3 is prevented.

A stud fastener 13 is provided at the top end of the stud shaft 3, and the stud fastener 13 seals the upper end of the pipe shaft 4 while communicating the upper connection pipe 5 with the pipe shaft 4.

The upper ends of all the chromatographic columns 2 are communicated with an upper liquid separating communicating vessel 6 through upper connecting pipes 5, the upper liquid separating communicating vessel 6 is communicated with an upper liquid inlet pipe 7, and each upper connecting pipe 5 is provided with a three-way valve 9. The lower ends of all the chromatographic columns 2 are communicated with a lower liquid separation communicating vessel 11 through lower connecting pipes 10, the lower liquid separation communicating vessel 11 is communicated with a lower liquid outlet pipe 12, and each lower connecting pipe 10 is provided with a three-way valve 9.

The upper ends of the two chromatographic columns 2 are communicated with an upper liquid outlet pipe 8 through a three-way valve 9, and the upper ends and the lower ends of the chromatographic columns 2 are communicated through the three-way valve 9 to form a series passage. The three-way valve 9 can control the connection or disconnection of the passages, thereby enabling different chromatographic columns 2 to participate in the operation, or connecting the chromatographic columns 2 in series.

The utility model discloses an in the concrete embodiment, chromatography column 2 is four in total, and wherein the upper end of two chromatography columns 2 has last drain pipe 8 through three-way valve 9 intercommunication, and the upper end that remains two chromatography columns 2 communicates each other through three-way valve 9, and the intercommunication has two chromatography column 2 lower extremes of last drain pipe 8 to communicate each other through three-way valve 9 with one of remaining two chromatography columns 2 respectively.

Specifically, four chromatographic columns 2 can be named as a first chromatographic column, a second chromatographic column, a third chromatographic column and a fourth chromatographic column, wherein the upper connecting pipes 5 of the first chromatographic column and the second chromatographic column are communicated with an upper liquid outlet pipe 8 through a three-way valve 9, and the upper connecting pipes 5 of the third chromatographic column and the fourth chromatographic column are communicated with each other through the three-way valve 9. At the lower end of the chromatography column 2, the lower connecting pipes 10 of the first and fourth chromatography columns are connected to each other through the three-way valve 9, while the connecting pipes of the second and third chromatography columns are connected to each other through the three-way valve 9.

Make the upper end of four chromatographic columns communicate with last liquid separator linker 6 simultaneously through three-way valve 9, close last drain pipe 8 on first chromatographic column and the second chromatographic column, and break off the connection of third chromatographic column and fourth chromatographic column upper end, simultaneously, make the lower extreme of four chromatographic columns communicate with lower liquid separator linker 11 simultaneously, break off the intercommunication of first chromatographic column and fourth chromatographic column lower extreme and the intercommunication between second chromatographic column and the third chromatographic column lower extreme, this moment, four chromatographic columns are parallel state.

The upper end of the first chromatographic column is communicated with the upper liquid separation communicating vessel 6 through the three-way valve 9, the lower end of the first chromatographic column is communicated with the lower end of the fourth chromatographic column, then the upper end of the fourth chromatographic column is communicated with the upper end of the third chromatographic column, the lower end of the third chromatographic column is communicated with the lower end of the second chromatographic column, finally the second chromatographic column is communicated with the upper liquid outlet pipe by 8 ways, and at the moment, the four chromatographic columns 2 are in a serial state.

Or the upper end and the lower end of one chromatographic column 2 can be independently controlled to be respectively communicated with the upper liquid separation communicating vessel 6 and the lower liquid separation communicating vessel 11, and the upper end and the lower end of other chromatographic columns 2 are disconnected, so that one chromatographic column 2 works independently.

The utility model discloses be in the same place a plurality of chromatography columns 2 are fixed, and pass through the pipeline intercommunication with a plurality of chromatography columns 2, realize multiple intercommunication mode through the switch of control three-way valve 9, if a plurality of chromatography columns 2 use or form respectively alone parallelly connected, or a plurality of chromatography columns 2 establish ties and use to be applicable to different chromatography modes.

For the chromatography of the size exclusion mode, the three-way valves 9 are adjusted to connect the chromatographic columns 2 in series, so that the use height of the chromatographic column 2 is improved, and the same effect of the chromatographic column 2 with a single column higher is achieved; by adjusting the upper and lower paths, the chromatographic columns 2 are changed into parallel connection, so that the pretreatment and balance time of the chromatographic columns 2 can be obviously shortened, and the time is saved for the molecular sieve chromatography process.

For the chromatography in the adsorption elution mode, after the chromatographic column 2 is well pretreated and balanced, the three-way valve 9 can be adjusted in an optimization stage, and is respectively connected with only one passage of the chromatographic column 2 at a time, so that the contrast optimization experiment of different conditions of a sample can be more conveniently carried out, after all the passages are respectively used, all the passages are opened, and the regeneration and pretreatment are carried out simultaneously, so that the preparation time is shortened, and the time utilization efficiency of a chromatography system is improved.

The utility model discloses can contain two chromatography columns 2 at least, the upper end and the lower extreme of two chromatography columns 2 all communicate each other through three-way valve 9 to go up drain pipe 8 on the three-way valve 9 of 2 upper ends of one of them chromatography column communicates, thereby can realize two chromatography columns 2 parallelly connected or establish ties.

When a plurality of chromatography columns 2 are provided, the number of the chromatography columns 2 is generally even, and then the upper and lower ends of all the chromatography columns 2 are alternately communicated through a three-way valve 9 to form a serial passage.

The utility model discloses a column cap 2-2, column cap pole 3, column cap mounting 2-4 and column cap fastener 13 of chromatography column 2 can both be convenient dismouting to be convenient for pack of chromatography column 2.

The utility model discloses can be used for adsorbing the medium of elution mode, improve the optimization efficiency and the 2 treatment effeciencies in chromatography column of process sieve condition, also can be used to the chromatography of size exclusion mode, chromatography column 2 no longer needs whole root chromatography to load very high, has reduced the dress post degree of difficulty to the efficiency of 2 processings of chromatography column has been improved. And simultaneously, the utility model discloses required space height is little, and the column casing passageway is closely concentrated, compares the space availability factor that has improved chromatographic column 2 than the chromatography in the past, can vacate more available spaces for laboratory and industrial production's biological product, bio-pharmaceuticals trade.

Claims

1. The utility model provides a multipurpose chromatography column, a serial communication port, includes the protective sheath be provided with a plurality of chromatography column in the protective sheath, the upper end of all chromatography columns is through last connecting pipe and last minute liquid linker intercommunication, it has last liquid inlet pipe to go up minute liquid linker intercommunication, is provided with the three-way valve on every last connecting pipe, and the lower extreme of all chromatography columns is through connecting pipe and lower minute liquid linker intercommunication down, divide liquid linker intercommunication down to have drain pipe down, be provided with the three-way valve under every on the connecting pipe, wherein two there is last drain pipe in the upper end of chromatography column through the three-way valve intercommunication, and the upper and lower both ends of chromatography column are passed through the three-way valve intercommunication and are formed the series connection way.

2. The multipurpose chromatographic column according to claim 1, comprising a cylinder body fixed in the protective sleeve, wherein an upper column head and a lower column head are respectively arranged at the upper end and the lower end of the cylinder body, a column head rod is arranged on the upper column head, a pipeline rod is connected in the column head rod in a penetrating manner, the lower end of the pipeline rod is communicated with the inner cavity of the chromatographic column, and the upper end of the pipeline rod is communicated with the upper connecting pipe.

3. The multipurpose chromatography column according to claim 2, wherein a column head fixing member is sleeved on the column head rod and fixed at the upper port of the cylinder body.

4. The multipurpose chromatography column according to claim 2, wherein a stud fastener is provided at a top end of the stud rod for communicating the upper connection pipe with the pipe line.

5. The multipurpose chromatographic column according to claim 1, wherein the number of the chromatographic columns is four, the upper ends of two of the chromatographic columns are communicated with the upper liquid outlet pipe through a three-way valve, the upper ends of the remaining two chromatographic columns are communicated with each other through a three-way valve, and the lower ends of the two chromatographic columns communicated with the upper liquid outlet pipe are respectively communicated with one of the remaining two chromatographic columns through a three-way valve.