CN218481475U - Liquid chromatography column - Google Patents

Abstract

The utility model relates to the technical field of chromatographic columns, in particular to a liquid chromatographic column, which comprises a body, a sieve plate and a fixed cap; the body is a hollow cylinder with openings at two axial ends, and comprises a body section, a fixed section and a connecting section which are coaxially connected in sequence along the axial direction of the body; at least part of the connecting section is covered by the screen plate; the fixed cap is provided with a containing cavity for containing the sieve plate, and the fixed section is connected with the fixed cap through threads. The sieve plate in the liquid chromatographic column provided by the utility model is easy to replace.

Description

Liquid chromatography column

Technical Field

The utility model relates to a chromatographic column technical field, in particular to liquid chromatographic column.

Background

The sieve plate is generally of a sheet structure in the existing chromatographic column, is placed in a containing cavity of a fixing cap, needs to be placed in the fixing cap in advance and then connected with a column body in the using process, and is stably and tightly fixed at a liquid outlet opening of the column body. However, in the actual working process, because the chromatographic column usually needs top washing, i.e. the target is resolved from the chromatographic packing under the high pressure condition by using a special mobile phase, the sieve plate is easy to be tightly attached to the fixed cap under the high pressure operation, and is difficult to be taken out from the cavity of the fixed cap, thereby seriously affecting the experiment or production.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects of the prior art, the utility model aims to solve the technical problems that: provides a liquid chromatographic column with a sieve plate convenient to replace.

In order to solve the technical problem, the utility model discloses a technical scheme be: the liquid chromatographic column comprises a body, a sieve plate and a fixing cap;

the body is a hollow cylinder with openings at two axial ends, and comprises a body section, a fixed section and a connecting section which are coaxially connected in sequence along the axial direction of the body;

at least part of the connecting section is covered by the screen plate;

the fixed cap is provided with a containing cavity for containing the sieve plate, and the fixed section is connected with the fixed cap through threads.

Wherein, the sieve plate comprises a filter disc and a cap body coated on the outer side of the filter disc.

Wherein the filter disc has filter pores of 3-5 μm.

The cap body comprises a fixing part and a sleeving part, the fixing part is used for fixing the filter disc and keeping the filter disc coaxial with the body, and the sleeving part extends along the axial direction of the filter disc.

Wherein the axial length of the socket joint part is smaller than that of the connecting section.

The fixing cap comprises a shell and a partition plate, the shell is a hollow cylinder with two open ends, the partition plate is arranged in an inner cavity of the shell and divides the inner cavity of the shell into the accommodating cavity and the outer cavity, and a liquid outlet pipe extending along the axial direction of the shell is arranged in the center of one side of the outer cavity of the partition plate.

Wherein the wall thickness of the cavity is smaller than that of the outer cavity.

The beneficial effects of the utility model reside in that: this application is through establishing the sieve cover the outside of linkage segment makes at least partial linkage segment quilt the sieve is wrapped up, makes the sieve can be connected with the linkage segment closely through this kind of setting promptly to avoid the closely emergence that leads to the sieve to be difficult to take out the scheduling problem in holding the chamber of locking nut at high pressure operation in-process sieve plate.

Drawings

FIG. 1 is an exploded view of a liquid chromatography column in accordance with an embodiment of the present invention;

FIG. 2 is a schematic diagram of a liquid chromatography column according to an embodiment of the present invention;

fig. 3 is a cross-sectional view of a liquid chromatography column in accordance with an embodiment of the present invention.

Description of reference numerals: 1. a body; 11. a body section; 12. a fixed section; 13. a connecting section; 2. a sieve plate; 21. a cap body; 22. a filter disc; 3. a fixed cap; 31. a housing; 32. an outer chamber; 33. an inner cavity; 34. a partition plate; 35. a liquid outlet pipe.

Detailed Description

In order to explain the technical content, the objects and the effects of the present invention in detail, the following description is made with reference to the accompanying drawings in combination with the embodiments.

Referring to fig. 1 to 3, a liquid chromatography column includes a body 1, a sieve plate 2, and a fixing cap 3; the body 1 is a hollow cylinder with openings at two axial ends, and the body 1 comprises a body section 11, a fixed section 12 and a connecting section 13 which are coaxially connected in sequence along the axial direction of the body 1; at least part of the connecting section 13 is covered by the screening deck 2; the fixing cap 3 is provided with a containing cavity for containing the sieve plate 2, and the fixing section 12 is connected with the fixing cap 3 through threads.

The connecting section 13 has a lower end opening of the body 1, so that the sieve plate 2 is coated on the connecting section 13 to block the chromatographic packing (stationary phase) in the inner cavity 33 of the body 1 from flowing out through the sieve plate 2, and at the same time, the surface of the sieve plate 2 is provided with holes, so that the mobile phase can flow out of the holes under high pressure through the holes.

The fixed cap 3 is connected with the fixed section 12 of the body 1 through threads, so that the sieve plate 2 is fixed in position when the sieve plate 2 is completely isolated from the outside, and the sieve plate 2 can be tightly attached to the lower end opening of the body 1.

The shape of the cavity should correspond to the shape of the screen plate 2, preferably such that the cavity wall of the cavity fits with the screen plate 2 with a small clearance fit. Of course, when the screen plate 2 is elastic, the two can be assembled by interference fit. Regardless of the manner of fit, it is ensured that the screen panel 2 is tightly clamped between the attachment section 13 and the bottom of the cavity of the retaining cap 3.

In one embodiment, the screen deck 2 includes a filter 22 and a cap 21 covering the outside of the filter 22. The filter disc 22 is mainly used for intercepting a stationary phase and allowing a mobile phase to flow out from a hole on the surface of the filter disc under a high-pressure condition, and the cap body 21 is mainly used for fixing the filter disc 22 so that the filter disc 22 keeps coaxial with the lower end opening of the body 1 and covers the surface of the connecting section 13. Specifically, the cap body 21 includes a fixing portion for fixing the filter 22 and keeping the filter 22 coaxial with the body 1, and a sleeve portion extending along an axial direction of the filter 22. Since filter 22 is generally a sheet-like structure, the securing portion preferably secures filter 22 by partially wrapping the edge of filter 22 in a nested manner, although the two may be connected using conventional techniques, such as bonding.

The diameter of the filter sheet 22 should be at least slightly larger than the diameter of the lower opening of the body 1, and the pore diameter of the surface filter pores is preferably 3-5 μm. Preferably, the filter disc is a porous metal filter disc formed by sintering and pressing stainless steel powder.

In a more preferred embodiment, the axial length of the socket is smaller than the axial length of the connecting section 13. At this time, when the sleeve is completely sleeved on the outer side of the connection section 13, the connection section 13 still maintains a certain margin length so that an operator can conveniently take the screen plate 2 off the connection section 13.

In an alternative embodiment, the surface of the socket part has continuous through holes distributed in a linear manner, and the linear distribution may be a straight line parallel to the central axis of the socket part or a spiral line taking the axis of the socket part as a spiral axis. Preferably, the continuous through hole extends from one axial end of the socket part to a position close to the fixing part. To pass through the continuous through-hole to form a break line facilitating tearing of the spigot. Through this kind of design in order realizing in special case, receive special conditions such as strong acid, alkali, high temperature partial melting down, when making it firmly adhere on the linkage segment, the accessible is torn this prefabricated at the broken line of cup jointing portion in order to get rid of the sieve that glues adnexingly fast.

In one embodiment, the fixing cap 3 includes a housing 31 and a partition 34, the housing 31 is a hollow cylinder with two open ends, the partition 34 is disposed in an inner cavity 33 of the housing 31 and divides the inner cavity 33 of the housing 31 into the accommodating cavity and an outer cavity 32, and the partition 34 has a liquid outlet pipe 35 extending axially along the housing 31 at a center of one side of the outer cavity 32. Wherein the outlet pipe 35 is used for guiding the mobile phase flowing out of the filter disc 22 out of the inner cavity 33.

Preferably, the wall thickness of the cavity is less than the wall thickness of the outer cavity 32.

It should be noted that, in the present application, both axial ends of the body 1 are open, so that both ends need to be sealed, and the other end can be sealed by the same structure as that described above, and other existing structures are not excluded.

Example 1

Referring to fig. 1 to 3, the liquid chromatography column comprises a body 1, a sieve plate 2 and a fixing cap 3;

the body 1 is a hollow cylinder with openings at two axial ends, and the body 1 comprises a body section 11, a fixed section 12 and a connecting section 13 which are coaxially connected in sequence along the axial direction of the body 1;

at least part of the connecting section 13 is covered by the screening deck 2;

the fixing cap 3 is provided with a cavity for accommodating the sieve plate 2, and the fixing section 12 is connected with the fixing cap 3 through threads;

the sieve plate 2 comprises a filter disc 22 and a cap body 21 covering the outer side of the filter disc 22;

the filter disc 22 is provided with filter holes of 3-5 mu m;

the cap body 21 comprises a fixing part and a sleeve part, the fixing part is used for fixing the filter disc 22 and keeping the filter disc 22 coaxial with the body 1, and the sleeve part extends along the axial direction of the filter disc 22;

the axial length of the sleeve joint part is smaller than that of the connecting section 13;

the fixing cap 3 comprises a shell 31 and a partition plate 34, the shell 31 is a hollow cylinder with two open ends, the partition plate 34 is arranged in an inner cavity 33 of the shell 31 and divides the inner cavity 33 of the shell 31 into the accommodating cavity and an outer cavity 32, and the partition plate 34 is provided with a liquid outlet pipe 35 extending along the axial direction of the shell 31 at the center of one side of the outer cavity 32;

the wall thickness of the cavity is less than the wall thickness of the outer cavity 32.

Specifically, during the assembly process, the screen plate 2 is firstly sleeved on the connecting section 13 of the body 1, and then the screen plate 2 is fixed by the fixing cap 3 through the solid section of the body 1.

During operation, the stationary phase is filled into the inner cavity 33 of the body 1 from the opening at the other end of the body 1, and the opening is sealed when the stationary phase is completely filled or filled to a specified height, volume or weight. The opening is now connected to a liquid chromatograph, and under high pressure, the mobile phase mixed with the target substance enters the body 1 through the opening, flows through the stationary phase under high pressure and exits through the lower end opening of the body 1, through the perforations in the filter disc 22 into the outlet pipe 35, and through the outlet pipe 35 into a collection bottle or other liquid collection device. The principles of operation of liquid chromatography columns are well known and will not be described in detail herein.

After the operation is finished, the fixing cap 3 is rotated to separate from the body 1, and at the moment, the sieve plate 2 is still tightly fixed on the connecting section 13, so that the sieve plate 2 is only required to be taken down from the connecting section 13 through a gap formed by the allowance length on the connecting section 13, and the disassembly of the liquid chromatography column is finished.

The above mentioned is only the embodiment of the present invention, and the patent scope of the present invention is not limited thereby, and all the equivalent transformations made by the contents of the specification and the drawings, or the direct or indirect application in the related technical field, are included in the patent protection scope of the present invention.

Claims (7)

1. The liquid chromatographic column is characterized by comprising a body, a sieve plate and a fixing cap;

the body is a hollow cylinder with openings at two axial ends, and comprises a body section, a fixed section and a connecting section which are coaxially connected in sequence along the axial direction of the body;

at least part of the connecting section is covered by the screen plate;

the fixed cap is provided with a containing cavity for containing the sieve plate, and the fixed section is connected with the fixed cap through threads.

2. The liquid chromatography column of claim 1, wherein the frit comprises a filter and a cap covering the outside of the filter.

3. The liquid chromatography column of claim 2, wherein the filter sheet has filter pores of 3 to 5 μm.

4. The liquid chromatography column of claim 2, wherein the cap comprises a fixing portion for fixing the filter sheet and holding the filter sheet coaxial with the body, and a socket portion extending in an axial direction of the filter sheet.

5. The liquid chromatography column of claim 4, wherein the axial length of the socket is less than the axial length of the connecting section.

6. The liquid chromatography column of claim 1, wherein the retaining cap comprises a housing and a partition, the housing is a hollow cylinder with two open ends, the partition is disposed in the inner cavity of the housing and divides the inner cavity of the housing into the receiving cavity and the outer cavity, and the partition has a liquid outlet pipe extending axially along the housing at the center of one side of the outer cavity.

7. The liquid chromatography column of claim 6, wherein the wall thickness of the receptacle is less than the wall thickness of the outer chamber.

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