CN218469432U - Be used for decellularized biological tissue gel freeze-drying device - Google Patents
Be used for decellularized biological tissue gel freeze-drying device Download PDFInfo
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- CN218469432U CN218469432U CN202221744748.9U CN202221744748U CN218469432U CN 218469432 U CN218469432 U CN 218469432U CN 202221744748 U CN202221744748 U CN 202221744748U CN 218469432 U CN218469432 U CN 218469432U
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- tray
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- 238000004108 freeze drying Methods 0.000 title claims abstract description 43
- 238000001035 drying Methods 0.000 claims abstract description 86
- 238000001816 cooling Methods 0.000 claims abstract description 27
- 238000010438 heat treatment Methods 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000000463 material Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 14
- 238000007710 freezing Methods 0.000 abstract description 8
- 230000008014 freezing Effects 0.000 abstract description 8
- 238000009777 vacuum freeze-drying Methods 0.000 abstract description 3
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 239000003206 sterilizing agent Substances 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 44
- 210000001519 tissue Anatomy 0.000 description 24
- 238000000859 sublimation Methods 0.000 description 5
- 230000008022 sublimation Effects 0.000 description 5
- 238000009833 condensation Methods 0.000 description 3
- 230000005494 condensation Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000010792 warming Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
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Abstract
The utility model relates to a gel freeze-drying device for acellular biological tissues, which comprises a cabinet body and a cabinet door; the cabinet body is equipped with placer: a drying tray, a drying tray cover, a drying rack and a level meter; a vacuum device: a vacuum pump and an air guide hole; a cooling device: a cooling pipeline, a compressor, an evaporator and a condensing plate; a heating device: a hot oil pipeline, a heating box and an oil pump; an electric control device: power control unit, temperature control unit, operation panel. Sterilizing a drying disc by introducing a sterilizing agent before freeze-drying, flatly paving the gel on the drying disc, placing the gel on a drying rack, adjusting the gel to be in a horizontal state, putting down a cover of the drying disc, and starting an electric control device to enable the drying rack to slowly rotate at a constant speed; the cooling device is used for pre-freezing and vacuum freeze-drying, and the heating device is used for providing heat in the drying process. The utility model solves the problems that the spreading of the gel on the drying tray is not uniform and the drying tray pollutes the gel; the dried object does not need to move in the freeze-drying process, and the problem of pollution to the dried object caused by manual movement is solved.
Description
Technical Field
The utility model relates to a freeze-drying device technical field, more specifically the be used for taking off cell biological tissue gel freeze-drying device that says so.
Background
Gels are increasingly used in tissue engineering because of their flexible, porous, freely changeable shape and strength-controllable properties. The acellular technology is utilized to directly remove cells and DNA which can cause immunological rejection reaction in tissues, and the rest acellular matrixes which retain most components of natural tissues are used as scaffold materials, so that the research on various aspects of tissue engineering, such as nerves, livers, bones, muscles, tendons, hearts and the like, is gradually increased; the combination of the two is an important development trend of acellular technology.
The freeze-drying technology is mostly selected for the preservation and transportation of the acellular biological tissue gel, the acellular gel is simply laid in a freeze-drying tray to be directly freeze-dried in the existing market, if the laid thickness is not uniform, the freeze-drying efficiency is directly influenced, and meanwhile, the acellular gel is easily polluted; the process from precooling to freeze-drying requires manual movement of the freeze-drying tray, and air contact easily causes pollution of the decellularized gel.
Therefore, how to provide a freeze-drying device for decellularized biological tissue gel, which is sanitary and convenient, is a problem to be solved urgently by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
The invention provides a decellularized biological tissue gel freeze-drying device, which is used for solving the technical problem.
In order to achieve the above object, the utility model provides a following technical scheme, the utility model provides a be used for taking off cell biological tissue gel freeze-drying device, including the cabinet body, cabinet door:
placing a device: the cabinet body is internally arranged in the middle of the cabinet body;
a vacuum device: the lower part of the cabinet body is internally provided with a switch;
a cooling device: the placing device is arranged on the right side of the placing device;
a heating device: the placing device is arranged on the left side of the placing device;
an electric control device: is arranged on the cabinet shell and is electrically connected with the placing device, the vacuum device, the cooling device and the heating device.
Preferably, in the above apparatus for freeze-drying gel for decellularized biological tissue, the placing apparatus comprises: the cabinet is internal to be equipped with the drying rack, can place a plurality of drying trays on the drying rack, can install the drying tray lid on the drying tray, drying tray bottom surface mid-mounting spirit level.
Preferably, in the above device for freeze-drying a gel of a decellularized biological tissue, an air inlet hole is arranged on the left side of the drying tray, an air outlet hole is arranged on the right side of the drying tray, and the air inlet hole and the air outlet hole can be manually closed by using a hole plug; the side surface of the drying plate is provided with scales.
Preferably, in the above device for freeze-drying gel for decellularized biological tissue, the cover of the drying tray can tightly cover the drying tray, so that the drying tray forms a closed space; the motor is connected to drying rack upper end, drying rack can slow at the uniform velocity rotation.
Preferably, in the above apparatus for freeze-drying gel for decellularized biological tissue, the vacuum apparatus comprises: the vacuum pump is arranged at the lower part of the cabinet body, one end of an exhaust pipe of the vacuum pump is connected with an air guide hole, and the air guide hole is arranged on one side of the cabinet body.
Preferably, in the above apparatus for freeze-drying gel of acellular biological tissue, the cooling device comprises: a cooling pipeline is arranged on the right side in the cabinet body and provided with a cold inlet end and a cold outlet end; the cooling pipeline is connected with an evaporator at the cold inlet end, a compressor at the cold outlet end, and the compressor is connected with the evaporator to form a circulating cooling system; the condensing plate is arranged in the upper part of the cabinet body.
Preferably, in the above apparatus for freeze-drying gel for decellularized biological tissue, the heating means comprises: a hot oil pipeline is arranged on the left side inside the cabinet body; the hot oil pipeline is provided with a heat inlet end and a heat outlet end; the hot oil pipeline hot-water inlet end is connected with an oil pump, the hot-water outlet end is connected with a heating box, and the heating box is connected with the oil pump to form a circulating heating system.
Preferably, in the above device for freeze-drying a gel of a decellularized biological tissue, an operating panel is provided on an outer shell of the cabinet; the operation panel is electrically connected with the power control unit and the temperature control unit.
Preferably, in the above device for freeze-drying gel for decellularized biological tissue, the cabinet door is made of a glass material with high temperature resistance and low temperature resistance.
According to the technical scheme, compared with the prior art, the utility model discloses the inlet port and the venthole of drying dish are let in sterilants such as ozone and are sterilized before carrying out freeze-drying, lay the gel on the drying dish and place on the drying rack and adjust to the horizontality, put down the drying dish lid, start electrically controlled device, make the drying rack at the uniform velocity slowly rotate, solve the inhomogeneous problem of gel thickness in the dish through slow rotation; the cooling device is used for pre-freezing and vacuum freeze-drying, and the condensing plate plays a role in absorbing water vapor in the freeze-drying process; the drying plate cover is opened after pre-freezing, the heating device provides heat in the freeze drying process, and frost on the inner wall of the cabinet body can be melted after freeze drying is finished. The utility model solves the problems that the spreading of the gel on the drying tray is not uniform and the drying tray pollutes the gel; the dried object does not need to move in the freeze-drying process, and the problem of pollution to the dried object caused by manual movement is solved.
The technical solution of the present invention is further described in detail by the accompanying drawings and examples.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of the internal structure of the present invention;
figure 2 attached is the structure schematic diagram of the drying plate of the utility model.
In the figure, 1, a cabinet body; 2. a cabinet door; 3. drying the disc; 4. a drying tray cover; 5. a drying rack; 6. a level gauge; 7. an air intake; 8. an air outlet; 9. a motor; 10. a vacuum pump; 11. An air vent; 12. a cooling pipeline; 13. a compressor; 14. an evaporator; 15. a condensing plate; 16. A cold inlet end; 17. a cold outlet end; 18. a hot oil line; 19. a temperature raising box; 20. an oil pump; 21. A heat inlet end; 22. a heat outlet end; 23. an operation panel; 24. and (4) plugging the hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.
In the present application, the terms "first", "second", "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance; the term "plurality" means two or more unless explicitly defined otherwise. The terms "mounted," "connected," "fixed," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a removable connection, or an integral connection; "coupled" may be direct or indirect through an intermediary. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the description of the present invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or unit indicated must have a specific direction, be constructed and operated in a specific orientation, and therefore, should not be construed as limiting the present invention.
In the description of the present specification, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Referring to fig. 1, the present invention provides a gel freeze-drying device for acellular biological tissue, comprising a cabinet body and a cabinet door:
placing a device: is arranged in the middle of the cabinet body 1;
a vacuum device: is arranged at the lower part of the cabinet body 1;
a cooling device: the placing device is arranged on the right side of the placing device;
a heating device: the placing device is arranged on the left side of the placing device;
an electric control device: is arranged on the shell of the cabinet body 1 and is electrically connected with the placing device, the vacuum device, the cooling device and the heating device.
According to the scheme, the gel of the decellularized biological tissue is flatly laid on the placing device, and the placing device is driven to rotate through the electric control device; the cooling device plays a role in pre-freezing and freeze-drying in the whole freeze-drying process; vacuum device is with cabinet body inside vacuumization state of taking out, freezes once more to the gel after freezing in advance and can make the inside moisture sublimation of gel, and the required heat of sublimation in-process is provided by heating device. According to the scheme, the acellular biological tissue gel does not need to be manually placed into the freeze-drying chamber from the pre-freezing chamber, so that the labor is saved, and the pollution of air to the gel is avoided.
In one embodiment, the placement device comprises: the cabinet body 1 is inside to be equipped with drying rack 5, can place a plurality of drying trays 3 on the drying rack 5, can install drying tray lid 4 on the drying tray 3, 3 basal surface mid-mounting levels 6 of drying tray.
In the scheme, in order to facilitate the arrangement of the drying tray 3, a plurality of support frames are arranged on the drying rack 5; the horizontal state of the drying tray 3 is ensured by the level gauge 6, and the problem that the drying tray 3 is uneven to influence the freeze-drying efficiency is reduced.
Referring to fig. 2, in one embodiment, an air inlet hole 7 is formed in the left side of the drying tray 3, an air outlet hole 8 is formed in the right side of the drying tray 3, and the air inlet hole 7 and the air outlet hole 8 can be manually closed by using a hole plug 24; the side surface of the drying disk 3 is provided with scales.
According to the scheme, the degerming agent is introduced from the air inlet 7 and discharged from the air outlet 8 before the gel is laid, so that the purpose of degerming is achieved, and the air outlet 8 of the air inlet 7 is closed to prevent the gel from leaking; the scale on the side surface of the drying tray 3 can clearly know the thickness of the gel of the decellularized biological tissue in the drying tray 3, and the water content of the gel of the decellularized biological tissue dried can be conveniently recorded in the freeze-drying process.
In one embodiment, the drying tray cover 4 can tightly cover the drying tray 3, so that the drying tray 3 forms a closed space; the motor 9 is connected to the upper end of the drying rack 5, and the drying rack 5 can rotate slowly at a constant speed.
Above-mentioned scheme provides inclosed space for the gel that takes off cells, and the gel is slowly at the uniform velocity of water gently in drying tray 3 can guarantee the thickness level of gel, makes to be cooled and heated more even simultaneously, and efficiency is higher.
In one embodiment, the vacuum apparatus comprises: the vacuum pump 10 is arranged at the lower part of the cabinet body, one end of an exhaust pipe of the vacuum pump 10 is connected with an air guide hole 11, and the air guide hole 11 is arranged on one side of the cabinet body 1.
Above-mentioned scheme provides the vacuum environment for cabinet 1 is inside, makes the moisture in the decellularization gel after freezing come out by sublimation.
In one embodiment, the cooling device includes: a cooling pipeline 12 is arranged on the right side inside the cabinet body 1, and the cooling pipeline 12 is provided with a cold inlet end 16 and a cold outlet end 17; the cooling pipeline inlet end 16 is connected with an evaporator 14, the outlet end 17 is connected with a compressor 13, and the compressor 13 is connected with the evaporator 14 to form a circulating cooling system; the condensation plate 15 is built in the upper part of the cabinet.
The scheme adopts a circulating refrigeration method, and uses a refrigerant as a refrigeration raw material; not only is convenient to operate, but also achieves the effect of a cold trap.
In one embodiment, the heating device comprises: a hot oil pipeline 18 is arranged at the left side inside the cabinet body 1; the hot oil pipeline 18 is provided with a hot inlet end 21 and a hot outlet end 22; the hot oil pipeline hot-water inlet end 21 is connected with an oil pump 20, the hot-water outlet end 22 is connected with a warming box 19, and the warming box 19 is connected with the oil pump 20 to form a circulating heating system.
The scheme adopts a circulating heating method, the temperature of oil is heated by the heating box 19, and hot oil is pumped into the heating pipeline 18 by the oil pump 20 for circulation; the heat required for sublimation is provided for the decellularized gel during lyophilization.
In one embodiment, the electrical control device comprises: an operating panel 23 is arranged on the shell of the cabinet body 1; the operation panel 23 is electrically connected with the power control unit and the temperature control unit.
The power transmission of all devices in the above-mentioned scheme electric control unit control cabinet body 1, the temperature of the temperature control unit control cabinet body 1 inside, through 23 control electric power of an operating panel and temperature, convenient simple.
In one embodiment, the cabinet door 2 is made of high temperature and low temperature resistant glass.
Above-mentioned scheme can observe the state of the inside acellular gel of the cabinet body 1 among the freeze-drying process clearly, can in time discover the problem that the acellular biological tissue gel appears among the freeze-drying process.
In order to further optimize the technical scheme, the distance between the condensation plate 15 and the drying disc 3 can be adjusted, so that water vapor and impurity particles can be better adsorbed conveniently.
In order to further optimize the technical scheme, the drying disc 3 has various sizes and depths, and the large-dose acellular gel is convenient to freeze dry.
In order to further optimize the technical scheme, the height of each stage of the drying rack 5 can be adjusted, so that the drying tray 3 can be conveniently placed.
In order to further optimize the technical scheme, sealing strips made of rubber materials are arranged on the periphery of the cabinet door 1, so that the vacuum state is guaranteed.
In order to further optimize the above technical solution, the motor 9 of each device is provided with an independent switch on the operation panel 23.
When the device works, firstly, a worker selects the drying disc 3 according to the dosage of the needed acellular gel, opens the air inlet 7 and the air outlet 8 of the drying disc 3, covers the drying disc cover 4 and sprays a degerming agent such as ozone into the drying disc; spreading the gel in a drying tray 3, covering a drying tray cover 4, and placing on a drying rack 5; observing a level gauge 6 below the drying disc 3, adjusting the state of the drying disc 3 until reaching the level, and closing the cabinet door 2; turning on a switch of a motor 9 on a control panel 23 to enable a drying rack 5 to drive a drying disc 3 to rotate slowly at a constant speed, and turning on a switch of a cooling device to pre-freeze; after pre-freezing, opening a vacuum pump 10 switch, and pumping the air in the cabinet body 1 to reach a vacuum state; opening a switch of the heating device to provide heat required by sublimation for the decellularized gel; the condensation plate 15 continuously adsorbs the water vapor thereon; and taking out the gel for freeze-drying after freeze-drying is finished, opening a heating device for defrosting, and preparing for next vacuum freeze-drying.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The utility model provides a be used for decellularization biological tissue gel freeze-drying device which characterized in that, includes the cabinet body, cabinet door:
placing a device: the cabinet body is internally arranged in the middle of the cabinet body;
a vacuum device: the lower part of the cabinet body is internally provided with a switch;
a cooling device: the placing device is arranged on the right side of the placing device;
a heating device: the placing device is arranged on the left side of the placing device;
an electric control device: the cooling device is arranged on the cabinet body shell and is electrically connected with the placing device, the vacuum device, the cooling device and the heating device; the placement device includes: the cabinet is internal to be equipped with the drying rack, can place a plurality of drying trays on the drying rack, can install the drying tray lid on the drying tray, drying tray bottom surface mid-mounting spirit level.
2. The freeze-drying device for the gel of the decellularized biological tissue as in claim 1, wherein an air inlet hole is formed on the left side of the drying tray, an air outlet hole is formed on the right side of the drying tray, and the air inlet hole and the air outlet hole can be manually closed by using a hole plug; the side surface of the drying plate is provided with scales.
3. The freeze-drying device for gel of decellularized biological tissue according to claim 2, wherein said drying tray cover can tightly cover said drying tray to form a closed space; the motor is connected to drying rack upper end, drying rack can slow at the uniform velocity rotation.
4. The apparatus of claim 1, wherein the vacuum means comprises: the vacuum pump is arranged at the lower part of the cabinet body, one end of an exhaust pipe of the vacuum pump is connected with an air guide hole, and the air guide hole is arranged on one side of the cabinet body.
5. The apparatus of claim 1, wherein the cooling means comprises: a cooling pipeline is arranged on the right side in the cabinet body and provided with a cold inlet end and a cold outlet end; the cooling pipeline is connected with an evaporator at the cold inlet end, a compressor at the cold outlet end, and the compressor is connected with the evaporator to form a circulating cooling system; the condensing plate is arranged in the upper part of the cabinet body.
6. The apparatus of claim 1, wherein the heating device comprises: a hot oil pipeline is arranged on the left side inside the cabinet body; the hot oil pipeline is provided with a heat inlet end and a heat outlet end; the hot oil pipeline hot-water inlet end is connected with an oil pump, the hot-water outlet end is connected with a heating box, and the heating box is connected with the oil pump to form a circulating heating system.
7. The apparatus of claim 1, wherein the electrical control device comprises: an operating panel is arranged on the shell of the cabinet body; the operation panel is electrically connected with the power control unit and the temperature control unit.
8. The freeze-drying device for gel of acellular biological tissue according to claim 1, wherein the cabinet door is made of glass material with high temperature resistance and low temperature resistance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221744748.9U CN218469432U (en) | 2022-07-05 | 2022-07-05 | Be used for decellularized biological tissue gel freeze-drying device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221744748.9U CN218469432U (en) | 2022-07-05 | 2022-07-05 | Be used for decellularized biological tissue gel freeze-drying device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218469432U true CN218469432U (en) | 2023-02-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221744748.9U Active CN218469432U (en) | 2022-07-05 | 2022-07-05 | Be used for decellularized biological tissue gel freeze-drying device |
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| Country | Link |
|---|---|
| CN (1) | CN218469432U (en) |
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2022
- 2022-07-05 CN CN202221744748.9U patent/CN218469432U/en active Active
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Denomination of utility model: A freeze-drying device for acellular biological tissue gel Granted publication date: 20230210 Pledgee: Suqian Haoyu Enterprise Management Partnership (L.P.) Pledgor: Hangzhou Beirong Biotechnology Co.,Ltd. Registration number: Y2024980000236 |