CN218452065U

CN218452065U - Isotope polypeptide purification device

Info

Publication number: CN218452065U
Application number: CN202222550410.6U
Authority: CN
Inventors: 伍昭化; 张岩; 沈燕飞
Original assignee: Jiangsu Zhengneng Isotope Co ltd
Current assignee: Jiangsu Zhengneng Isotope Co ltd
Priority date: 2022-09-27
Filing date: 2022-09-27
Publication date: 2023-02-07
Anticipated expiration: 2032-09-27

Abstract

The utility model discloses a purification device of isotope polypeptide relates to machining equipment technical field, comprising a main body, the inside of main part is provided with first purification device, the below fixed mounting of main part has stabilising arrangement, the right lower extreme fixed mounting of main part has the collecting pipe, the lower extreme of collecting pipe is provided with the receiving flask, the upper end fixed mounting of main part has the support, the right side fixed mounting of support has the mount, the right side fixed mounting of mount has third purification device. The utility model discloses the purification net all comprises the gel material, and the gel has the effect of filtering protein, makes the purification effect better, and the filter screen is a functional, and filter equipment comprises the material that the four layers have good filter effect, makes the isotope polypeptide half penetrate thin purifying, can filter out the great impurity of some molecules, and the valve is used for controlling the speed that the isotope polypeptide flows, more convenient and follow-up operation.

Description

Isotope polypeptide purification device

Technical Field

The utility model relates to a machining equipment technical field, concretely relates to isotope polypeptide's purification device.

Background

With the wider and deeper application of polypeptides in the field of biomedicine, the requirements of labeled and modified polypeptide types are more and more, and the quality requirement is higher and higher. Stable isotope labels are one of those which are typical. The stable isotope labeling tracing can realize the research of peptide metabolic pathway and trace the change condition of the position and the quantity of the polypeptide containing the isotope labeling in vivo or in vitro at any time. The isotope polypeptide has the advantages of high sensitivity, simple positioning, accurate quantification and the like, so that the isotope polypeptide gets more and more extensive attention in the medical and biochemical fields.

The following problems exist in the prior art:

1. when the existing isotope polypeptide purification device is used, because the concentration of isotope polypeptides is low and the components are complex, the required isotope polypeptide compound can be obtained only by the purification and separation procedures of the isotope polypeptides, and the purification degree of the obtained isotope polypeptides is not high;

2. when the existing isotope polypeptide purification device is used, the purification operation process is complicated and complicated, and a large amount of time is consumed in the process.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model discloses the technical scheme who adopts is:

the utility model provides a purification device of isotope polypeptide, includes the main part, the inside of main part is provided with first purification device, the below fixed mounting of main part has stabilising arrangement, the right lower extreme fixed mounting of main part has the collecting pipe, the lower extreme of collecting pipe is provided with the receiving flask, the upper end fixed mounting of main part has the support, the right side fixed mounting of support has the mount, the right side fixed mounting of mount has third purification device, the lower extreme of third purification device is provided with second purification device.

The utility model discloses technical scheme's further improvement lies in: first purification device is including the fixed block, one side fixed mounting of fixed block has the fixed column, the right-hand member of fixed column is provided with third purification net, the upper end of third purification net is provided with second purification net, the upper end of second purification net is provided with first purification net, the lower extreme of third purification net is provided with fifth purification net, the lower extreme of fifth purification net is provided with the fourth purification net.

The utility model discloses technical scheme's further improvement lies in: and the two sides of the third purification net are fixedly connected with one side of the fixed column.

The utility model discloses technical scheme's further improvement lies in: the third purification device comprises a first connecting pipe, a filter device is arranged at the upper end of the first connecting pipe, and a feed inlet is formed in the upper end of the filter device.

The utility model discloses technical scheme's further improvement lies in: the lower extreme of feed inlet can be dismantled with filter equipment's upper end and be connected, filter equipment's lower extreme can be dismantled with first connecting pipe and be connected.

The utility model discloses technical scheme's further improvement lies in: the second purification device comprises a receiving port, a filter screen is fixedly connected with the lower end of the receiving port, a discharge port is fixedly connected with the lower end of the filter screen, a valve is arranged at the lower end of the discharge port, and a second connecting pipe is arranged at the lower end of the valve.

The utility model discloses technical scheme's further improvement lies in: the lower end of the discharge hole is detachably connected with the upper end of a second connecting pipe, and the middle of the second connecting pipe is fixedly connected with the valve.

Owing to adopted above-mentioned technical scheme, the utility model discloses relative prior art, the technological progress who gains is:

1. the utility model provides an isotope polypeptide's purification device, under the combined action through first purification net, second purification net, third purification net, fourth purification net and fifth purification net, realize purification isotope polypeptide, purification net all comprises the gel material, and the gel has fine filtration protein's effect, and isotope polypeptide obtains pure isotope polypeptide through the purification of multilayer purification net.

2. The utility model provides an isotope polypeptide's purification device, under the combined action through four layers of filter screens among the filter equipment, realized the not pure problem inadequately of extraction of isotope polypeptide, filter equipment comprises the material that four layers have good filter effect, makes isotope polypeptide get rid of some big impurities through filtering before purifying, makes the purification effect better.

3. The utility model provides an isotope polypeptide's purification device, under the combined action through filter screen and valve, solved isotope polypeptide's purification device operation complicated problem, the filter screen is the semi-permeable thin of a performance, can cross the great impurity of some molecules of filtering, to isotope polypeptide further purification, the valve is used for controlling the speed that isotope polypeptide flows, more convenient and follow-up operation.

Drawings

Fig. 1 is a schematic structural view of an apparatus for purifying an isotope polypeptide of the present invention;

FIG. 2 is a schematic structural view of a first purifying apparatus of the present invention;

FIG. 3 is a schematic structural view of a third purifying apparatus according to the present invention;

fig. 4 is a schematic structural diagram of a second purification apparatus of the present invention.

In the figure: 1. a main body; 2. a first purification device; 3. a stabilizing device; 4. a collection bottle; 5. a collection pipe; 6. a second purification device; 7. a third purifying device; 8. a support; 9. a fixed mount; 21. a fixed block; 22. a first purification net; 23. a second purification net; 24. fixing a column; 25. a third purification net; 26. a fourth purification net; 27. a fifth purification net; 71. a first connecting pipe; 72. a filtration device; 73. a feed inlet; 61. a receiving port; 62. a filter screen; 63. a discharge port; 64. a second connection pipe; 65. and (4) a valve.

Detailed Description

The present invention will be described in further detail with reference to the following examples:

example 1

As shown in fig. 1-4, the utility model provides an isotope polypeptide's purification device, including main part 1, main part 1's inside is provided with first purification device 2, and main part 1's below fixed mounting has stabilising arrangement 3, and main part 1's right lower extreme fixed mounting has collecting pipe 5, and collecting pipe 5's lower extreme is provided with receiving flask 4, and main part 1's upper end fixed mounting has support 8, and support 8's right side fixed mounting has mount 9, and mount 9's right side fixed mounting has third purification device 7, and the lower extreme of third purification device 7 is provided with second purification device 6.

Example 2

As shown in fig. 1-4, on the basis of embodiment 1, the utility model provides a technical solution: preferably, the first purification device 2 includes a fixed block 21, a fixed column 24 is fixedly mounted on one side of the fixed block 21, a third purification net 25 is arranged at the right end of the fixed column 24, a second purification net 23 is arranged at the upper end of the third purification net 25, a first purification net 22 is arranged at the upper end of the second purification net 23, a fifth purification net 27 is arranged at the lower end of the third purification net 25, a fourth purification net 26 is arranged at the lower end of the fifth purification net 27, and two sides of the third purification net 25 are fixedly connected with one side of the fixed column 24.

In this embodiment, the first purification net 22, the second purification net 23, the third purification net 25, the fourth purification net 26, and the fifth purification net 27 are all made of gel material, the gel has a good effect of filtering proteins, and the isotope polypeptide is purified by the multi-layer purification net to obtain pure isotope polypeptide.

Example 3

As shown in fig. 1-4, on the basis of embodiment 1, the utility model provides a technical solution: preferably, the third purifying device 7 comprises a first connecting pipe 71, a filtering device 72 is arranged at the upper end of the first connecting pipe 71, a feeding hole 73 is arranged at the upper end of the filtering device 72, the lower end of the feeding hole 73 is detachably connected with the upper end of the filtering device 72, and the lower end of the filtering device 72 is detachably connected with the first connecting pipe 71.

In this embodiment, the filtering device 72 is made of four layers of materials with good filtering effect, so that the isotope polypeptides are filtered to remove some large impurities before purification, and the purification effect is better.

Example 4

As shown in fig. 1-4, on the basis of embodiment 1, the utility model provides a technical solution: preferably, the second purifying device 6 includes a receiving port 61, a filtering net 62 is fixedly connected to the lower end of the receiving port 61, a discharging port 63 is fixedly connected to the lower end of the filtering net 62, a valve 65 is arranged at the lower end of the discharging port 63, a second connecting pipe 64 is arranged at the lower end of the valve 65, the lower end of the discharging port 63 is detachably connected to the upper end of the second connecting pipe 64, and the middle part of the second connecting pipe 64 is fixedly connected to the valve 65.

In this embodiment, the filtering net 62 is a semi-permeable film with good performance, and can filter some impurities with large molecules, further purify the isotope polypeptide, and the valve 65 is used to control the flowing speed of the isotope polypeptide, so as to facilitate the subsequent operations.

The working principle of the device for purifying the isotope polypeptide will be described in detail below.

As shown in fig. 1-4, the first purifying net 22, the second purifying net 23, the third purifying net 25, the fourth purifying net 26 and the fifth purifying net 27 are all made of gel material, the gel has a good protein filtering effect, the isotope polypeptide is purified by the multi-layer purifying net to obtain pure isotope polypeptide, the filtering device 72 is made of four layers of material with good filtering effect, so that the isotope polypeptide is filtered to remove some large impurities before purification, the purification effect is better, the filtering net 62 is a semi-permeable film with good performance, which can filter some large molecular impurities, further purify the isotope polypeptide, the valve 65 is used to control the flow speed of the isotope polypeptide, and is more convenient for subsequent operations.

The present invention has been described in detail with reference to the above general description, but it will be apparent to one of ordinary skill in the art that modifications and improvements can be made to the invention. Therefore, modifications or improvements without departing from the spirit of the invention are within the scope of the invention.

Claims

1. An isotopic polypeptide purification apparatus comprising a body (1), characterized by: the inside of main part (1) is provided with first purification device (2), the below fixed mounting of main part (1) has stabilising arrangement (3), the right lower extreme fixed mounting of main part (1) has collecting pipe (5), the lower extreme of collecting pipe (5) is provided with receiving flask (4), the upper end fixed mounting of main part (1) has support (8), the right side fixed mounting of support (8) has mount (9), the right side fixed mounting of mount (9) has third purification device (7), the lower extreme of third purification device (7) is provided with second purification device (6).

2. An isotopic polypeptide purification apparatus as claimed in claim 1, wherein: first purification device (2) is including fixed block (21), one side fixed mounting of fixed block (21) has fixed column (24), the right-hand member of fixed column (24) is provided with third purification net (25), the upper end of third purification net (25) is provided with second purification net (23), the upper end of second purification net (23) is provided with first purification net (22), the lower extreme of third purification net (25) is provided with fifth purification net (27), the lower extreme of fifth purification net (27) is provided with fourth purification net (26).

3. An isotopic polypeptide purification apparatus as claimed in claim 2, wherein: and two sides of the third purification net (25) are fixedly connected with one side of the fixed column (24).

4. An isotopic polypeptide purification apparatus as claimed in claim 1, wherein: the third purifying device (7) comprises a first connecting pipe (71), a filtering device (72) is arranged at the upper end of the first connecting pipe (71), and a feeding hole (73) is formed in the upper end of the filtering device (72).

5. An isotopic polypeptide purification apparatus as claimed in claim 4, wherein: the lower end of the feed inlet (73) is detachably connected with the upper end of the filtering device (72), and the lower end of the filtering device (72) is detachably connected with the first connecting pipe (71).

6. An isotopic polypeptide purification apparatus as claimed in claim 1, wherein: second purification device (6) is including receiving port (61), lower extreme fixedly connected with filter screen (62) of receiving port (61), lower extreme fixedly connected with discharge gate (63) of filter screen (62), the lower extreme of discharge gate (63) is provided with valve (65), the lower extreme be provided with second connecting pipe (64).

7. An isotopic polypeptide purification apparatus as claimed in claim 6, wherein: the lower end of the discharge hole (63) is detachably connected with the upper end of a second connecting pipe (64), and the middle part of the second connecting pipe (64) is fixedly connected with a valve (65).