CN218435753U

CN218435753U - Fluorescent signal detection device of real-time fluorescent quantitative PCR instrument

Info

Publication number: CN218435753U
Application number: CN202222088957.9U
Authority: CN
Inventors: 曹逸然; 吴烨
Original assignee: Yixing Baiao Precision Nuclear Biotechnology Co ltd
Current assignee: Yixing Baiao Precision Nuclear Biotechnology Co ltd
Priority date: 2022-08-10
Filing date: 2022-08-10
Publication date: 2023-02-03
Anticipated expiration: 2032-08-10

Abstract

The utility model discloses a fluorescence signal detection device of a real-time fluorescence quantitative PCR instrument, which comprises a detection cavity, wherein the detection cavity is arranged inside a device main body, and a touch screen is arranged above the device main body; a first T-shaped mounting frame and a second T-shaped mounting frame are arranged in the detection cavity, sliding grooves are formed in the top of the detection cavity and the lower end of the first T-shaped mounting frame, a first threaded rod and a second threaded rod are arranged in the sliding grooves, and a fluorescence detector is arranged below the second T-shaped mounting frame; first motor and second motor, it sets up respectively the inside of device main part and one side of first T type mounting bracket, this fluorescence signal detection device can be adjusted according to the user demand fast for fluorescence signal detection mechanism can be accurate remove to the sample top, avoids the angle difference to fluorescence signal detection interference, can detect a plurality of samples fast, improves fluorescence signal detection effect.

Description

Fluorescent signal detection device of real-time fluorescent quantitative PCR instrument

Technical Field

The utility model relates to a fluorescence quantitative PCR appearance technical field specifically is real-time fluorescence quantitative PCR appearance fluorescence signal detection device.

Background

The real-time fluorescence quantitative PCR technology can realize quantitative analysis of a DNA template, has important significance for molecular biology research, medical research and the like, and is widely applied to various medical institutions, universities and research institutes, CDC, inspection and quarantine bureaus, veterinary stations, food enterprises, dairy plants and the like. The real-time fluorescence quantitative pcr instrument consists of a fluorescence quantitative system and a computer, is used for monitoring the fluorescence of a cyclic process, and is connected with real-time equipment, and the computer collects fluorescence data. The fluorescence signal detection mechanism is an important component of a real-time fluorescence quantitative PCR instrument, and fluorescence detection is a commonly used detection technology and is used for carrying out qualitative and quantitative detection on an analyte by utilizing the characteristic that the analyte can generate fluorescence under the excitation of specific wavelength light.

The Chinese patent publication No. CN201785391U, the granted publication No. 2011/04/06/2011, a fluorescence signal detection device of a real-time fluorescence quantitative PCR instrument, wherein one end of a lead screw is fixedly connected with a transmission device through a bearing arranged on a guide rail, a sliding block and the guide rail form sliding fit and are arranged together with the lead screw, and the sliding block is fixedly connected with a second stepping motor; in the light path module shell, light emitted by a light source sequentially passes through a bicolor spectroscope, a plane mirror, a lens and a light outlet hole to be emitted into a detected sample, and fluorescent groups in the detected sample are excited to generate fluorescence which is emitted into a receiving end of a photomultiplier through the light outlet hole, the lens, the plane mirror, the bicolor spectroscope and a filter wheel; an output shaft of the second stepping motor is fixed with the light path module shell; the pulse signal input ends and the direction signal input ends of the first subdivision driver and the second subdivision driver are connected with the corresponding output ends of the controller, and the output end of the first subdivision driver is connected with the transmission device; the output end of the second subdivision driver is connected with the second stepping motor. The utility model discloses compact structure, light path system are simple reliable.

The above prior art solutions have the following drawbacks: when the fluorescent signal detection device detects a plurality of rows of parallel samples, the fluorescent signal detection mechanism is inconvenient to adjust, and the angular deviation between the fluorescent signal detection mechanism and the samples is inconvenient to detect the fluorescent signals, so that the fluorescent signal detection efficiency is reduced, and the using requirements cannot be met.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a real-time fluorescence quantitative PCR appearance fluorescence signal detection device to when solving the above-mentioned sample that provides fluorescence signal detection device and detecting the multirow side by side in the background art, the inconvenient regulation of fluorescence signal detection mechanism, the inconvenient fluorescence signal that carries on of angular deviation between fluorescence signal detection mechanism and the sample detects, has reduced fluorescence signal detection efficiency, can not satisfy user demand's problem.

In order to achieve the above purpose, the utility model provides a following technical scheme: the fluorescent signal detection device of the real-time fluorescent quantitative PCR instrument comprises a device main body;

the detection cavity is arranged inside the device main body, a touch screen is arranged above the device main body, the touch screen is connected with the device main body through a screw, a power line is arranged on one side of the device main body, and the power line and the device main body are connected into an integral structure;

a first T-shaped mounting frame and a second T-shaped mounting frame are arranged inside the detection cavity, sliding grooves are formed in the top of the detection cavity and the lower end of the first T-shaped mounting frame, the first T-shaped mounting frame and the second T-shaped mounting frame are respectively in sliding connection with the device main body and the first T-shaped mounting frame, threaded holes are formed in the first T-shaped mounting frame and the second T-shaped mounting frame, a first threaded rod and a second threaded rod are arranged inside the sliding grooves and are respectively in rotating connection with the device main body and the first T-shaped mounting frame, the first threaded rod and the second threaded rod are respectively in threaded connection with the first T-shaped mounting frame and the second T-shaped mounting frame, a fluorescence detector is arranged below the second T-shaped mounting frame, and the fluorescence detector is connected with the second T-shaped mounting frame through screws;

first motor and second motor, it sets up respectively the inside of device main part and one side of first T type mounting bracket, and first motor and second motor pass through screw connection with device main part and first T type mounting bracket respectively, the output of first motor and second motor is connected as an organic whole structure with first threaded rod and second threaded rod respectively.

Preferably, the fluorescence detector includes an optical fiber connector, a light guide bundle, a first filter set, a laser emitter, a dichroic mirror, a spectrometer, and a second filter set, the light guide bundle and the second filter set are connected to the optical fiber connector to form an integrated structure, the first filter set is disposed above the light guide bundle, the laser emitter is disposed above the first filter set, the dichroic mirror is disposed above the second filter set, and the spectrometer is disposed on one side of the dichroic mirror.

Preferably, the inside in detection chamber is provided with puts the thing board, the top of putting the thing board is provided with the sample box, and the sample box with put the thing board joggle, be provided with the reagent pipe on the sample box, and the reagent pipe equidistance sets up on the sample box.

Preferably, one side of the device main body is provided with a bin door, the bin door is rotatably connected with the device main body, the lower end of the bin door is provided with a handle groove, the bin door is provided with a visual window, and the visual window and the bin door are arranged into an integral structure.

Preferably, put thing board and device main part sliding connection, the below of putting the thing board is provided with electric telescopic handle, and electric telescopic handle is provided with two, and two electric telescopic handle settings are at the both ends of putting the thing board, electric telescopic handle and device main part with put thing board fixed connection.

Preferably, the upper end of first T type mounting bracket is provided with the guide way, the top of first T type mounting bracket is provided with the guide bar, and guide bar and device main part fixed connection, guide bar and first T type mounting bracket sliding connection.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the utility model discloses a device is through the setting of first motor, the second motor, first T type mounting bracket and second T type mounting bracket, first motor drives first threaded rod rotatory, along with the rotatory first T type mounting bracket of drive of first threaded rod moves along first threaded rod, realize fluorescence detector lateral adjustment, the second motor drives the second threaded rod rotatory, along with the rotatory second T type mounting bracket of drive of second threaded rod moves along the second threaded rod, realize fluorescence detector vertical adjustment, make fluorescence detector can be fast accurate carry out fluorescence signal detection to a plurality of reagent pipes on the sample box;

2. the utility model discloses a device is through putting thing board and electric telescopic handle's setting, installs the sample box and puts the thing board on, drives under electric telescopic handle's effect and puts the thing board and go up and down, along with putting the interval between the lift adjustment fluorescence detector of thing board and the reagent pipe, can satisfy different fluorescence signal detection demands.

Drawings

FIG. 1 is a schematic view of the structure of the present invention;

fig. 2 is a cross-sectional view of the present invention;

FIG. 3 is a diagram of a fluorescence detector according to the present invention;

FIG. 4 is a structural diagram of an adjusting mechanism of the present invention;

fig. 5 is a bottom view of the first T-shaped mounting frame and the second T-shaped mounting frame connected to the device body.

In the figure: 1. a device main body; 2. a power line; 3. a touch screen; 4. a bin gate; 5. a visual window; 6. a handle groove; 7. a detection chamber; 8. a storage plate; 9. a sample cartridge; 10. a reagent tube; 11. a fluorescence detector; 12. a chute; 13. a first T-shaped mounting bracket; 14. a second T-shaped mounting bracket; 15. a first threaded rod; 16. a first motor; 17. an optical fiber splice; 18. a light-guiding bundle; 19. a first filter set; 20. a laser transmitter; 21. a dichroic mirror; 22. a spectrometer; 23. a second motor; 24. a threaded hole; 25. a second threaded rod; 26. a second filter set; 27. a guide groove; 28. a guide bar; 29. an electric telescopic rod.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-5, the present invention provides an embodiment: the fluorescent signal detection device of the real-time fluorescent quantitative PCR instrument comprises a device main body 1;

the detection cavity 7 is arranged inside the device main body 1, the touch screen 3 is arranged above the device main body 1, the touch screen 3 is connected with the device main body 1 through screws, the power line 2 is arranged on one side of the device main body 1, the power line 2 is connected with the device main body 1 into an integral structure, and the touch screen 3 can set data of the fluorescence signal detection device and can also display the detected data;

a first T-shaped mounting frame 13 and a second T-shaped mounting frame 14 are arranged in the detection cavity 7, a sliding groove 12 is formed in the top of the detection cavity 7 and the lower end of the first T-shaped mounting frame 13, the first T-shaped mounting frame 13 and the second T-shaped mounting frame 14 are respectively connected with the device body 1 and the first T-shaped mounting frame 13 in a sliding mode, threaded holes 24 are formed in the first T-shaped mounting frame 13 and the second T-shaped mounting frame 14, a first threaded rod 15 and a second threaded rod 25 are arranged in the sliding groove 12, the first threaded rod 15 and the second threaded rod 25 are respectively connected with the device body 1 and the first T-shaped mounting frame 13 in a rotating mode, the first threaded rod 15 and the second threaded rod 25 are respectively connected with the first T-shaped mounting frame 13 and the second T-shaped mounting frame 14 in a threaded mode, a fluorescence detector 11 is arranged below the second T-shaped mounting frame 14, the fluorescence detector 11 is connected with the second T-shaped mounting frame 14 through screws, the fluorescence detector 11 is used for projecting excitation light and collecting fluorescence signals generated after excitation, and therefore detection of fluorescence signals is achieved;

the first motor 16 and the second motor 23 are respectively arranged inside the device body 1 and on one side of the first T-shaped mounting frame 13, the first motor 16 and the second motor 23 are respectively connected with the device body 1 and the first T-shaped mounting frame 13 through screws, the output ends of the first motor 16 and the second motor 23 are respectively connected with the first threaded rod 15 and the second threaded rod 25 to form an integral structure, and the transverse and longitudinal movement of the fluorescence detector 11 is respectively realized under the action of the first motor 16 and the second motor 23, so that the fluorescence detector 11 can quickly detect the reagent tube 10 on the sample box 9.

Referring to fig. 3, the fluorescence detector 11 includes an optical fiber connector 17, a light guide beam 18, a first filter set 19, a laser emitter 20, a dichroic mirror 21, a spectrometer 22, and a second filter set 26, the light guide beam 18 and the second filter set 26 are connected with the optical fiber connector 17 to form an integrated structure, the first filter set 19 is disposed above the light guide beam 18, the laser emitter 20 is disposed above the first filter set 19, the dichroic mirror 21 is disposed above the second filter set 26, the spectrometer 22 is disposed at one side of the dichroic mirror 21, a laser transmission mechanism is formed by the light guide beam 18, the first filter set 19, and the laser emitter 20, and is used for performing laser irradiation on a sample, and the second filter set 26, the dichroic mirror 21, and the spectrometer 22 form a fluorescence signal collection mechanism for conducting fluorescence signals generated after excitation, thereby realizing fluorescence signal detection.

Referring to fig. 2, a storage plate 8 is disposed inside the detection chamber 7, a sample box 9 is disposed above the storage plate 8, the sample box 9 is jogged with the storage plate 8, a reagent tube 10 is disposed on the sample box 9, and the reagent tube 10 is equidistantly disposed on the sample box 9.

Referring to fig. 1, a bin door 4 is disposed on one side of a device body 1, the bin door 4 is rotatably connected with the device body 1, a handle slot 6 is disposed at the lower end of the bin door 4, a visual window 5 is disposed on the bin door 4, the visual window 5 and the bin door 4 are integrated, sample loading and unloading are facilitated through opening and closing of the bin door 4, and the visual window 5 enables observation from the outside in a detection process.

Referring to fig. 2, the object placing plate 8 is slidably connected to the device main body 1, two electric telescopic rods 29 are arranged below the object placing plate 8, the two electric telescopic rods 29 are arranged at two ends of the object placing plate 8, the electric telescopic rods 29 are fixedly connected with the device main body 1 and the object placing plate 8, the object placing plate 8 is driven to lift through the electric telescopic rods 29, and the distance between the reagent tube 10 and the fluorescence detector 11 is adjusted along with the lifting of the object placing plate 8.

Referring to fig. 4 and 5, a guide groove 27 is formed at the upper end of the first T-shaped mounting frame 13, a guide rod 28 is arranged above the first T-shaped mounting frame 13, the guide rod 28 is fixedly connected with the device body 1, the guide rod 28 is slidably connected with the first T-shaped mounting frame 13, the guide groove 27 and the guide rod 28 can be used for guiding and limiting the moving first T-shaped mounting frame 13, and the moving stability of the first T-shaped mounting frame 13 is ensured.

The working principle is as follows: the bin door 4 is opened, the sample is placed on the object placing plate 8, then the bin door 4 is closed, the electric telescopic rod 29 is driven to drive the object placing plate 8 to lift, and the distance between the sample and the fluorescence detector 11 is adjusted appropriately; data setting is performed by means of the touch screen 3, a light source emitted by the laser emitter 20 is processed by the first filter set 19 and the light guide beam 18 and then irradiated to a sample, and fluorescence generated by the sample after being irradiated by the laser is transmitted to the spectrometer 22 through the second filter set 26 and the dichroic mirror 21 for processing, so that fluorescence signal detection is realized. Open first motor 16 and drive first threaded rod 15 rotatory, along with the rotatory fluorescence detector 11 lateral shifting that drives of first threaded rod 15, open second motor 23 and drive second threaded rod 25 rotatory, along with the rotatory fluorescence detector 11 longitudinal movement that drives of second threaded rod 25 for fluorescence detector 11 can be fast accurate carry out fluorescence signal to a plurality of reagent pipes 10 and detect, improve fluorescence signal detection efficiency.

Those not described in detail in this specification are within the skill of the art.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Real-time fluorescence quantitative PCR appearance fluorescence signal detection device, including device main part (1), its characterized in that:

the detection cavity (7) is arranged inside the device main body (1), the touch screen (3) is arranged above the device main body (1), the touch screen (3) is connected with the device main body (1) through screws, the power line (2) is arranged on one side of the device main body (1), and the power line (2) and the device main body (1) are connected into an integral structure;

a first T-shaped mounting frame (13) and a second T-shaped mounting frame (14) are arranged inside the detection cavity (7), sliding grooves (12) are formed in the top of the detection cavity (7) and the lower end of the first T-shaped mounting frame (13), the first T-shaped mounting frame (13) and the second T-shaped mounting frame (14) are respectively in sliding connection with the device main body (1) and the first T-shaped mounting frame (13), threaded holes (24) are formed in the first T-shaped mounting frame (13) and the second T-shaped mounting frame (14), a first threaded rod (15) and a second threaded rod (25) are arranged inside the sliding grooves (12), the first threaded rod (15) and the second threaded rod (25) are respectively in rotating connection with the device main body (1) and the first T-shaped mounting frame (13), the first mounting frame (15) and the second mounting frame (25) are respectively in threaded connection with the first T-shaped mounting frame (13) and the second T-shaped mounting frame (14), a fluorescence detector (11) is arranged below the second T-shaped mounting frame (14), and a fluorescence detector (11) is connected with a T-shaped screw (14);

first motor (16) and second motor (23), it sets up respectively the inside of device main part (1) and one side of first T type mounting bracket (13), and first motor (16) and second motor (23) pass through the screw connection with device main part (1) and first T type mounting bracket (13) respectively, the output of first motor (16) and second motor (23) is connected as an organic whole structure with first threaded rod (15) and second threaded rod (25) respectively.

2. The fluorescence signal detection device of the real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: fluorescence detector (11) are including optical fiber joint (17), light beam (18), first filter group (19), laser emitter (20), dichroic mirror (21), spectrum appearance (22) and second filter group (26), light beam (18) and second filter group (26) are connected as an organic whole structure with optical fiber joint (17), first filter group (19) set up in the top of light beam (18), and laser emitter (20) set up the top at first filter group (19), dichroic mirror (21) set up the top at second filter group (26), and spectrum appearance (22) set up the one side at dichroic mirror (21).

3. The fluorescence signal detection device of the real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: the inside of detection chamber (7) is provided with puts thing board (8), the top of putting thing board (8) is provided with sample box (9), and sample box (9) with put thing board (8) joggle, be provided with reagent pipe (10) on sample box (9), and reagent pipe (10) equidistance setting on sample box (9).

4. The fluorescence signal detection device of the real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: one side of the device main body (1) is provided with a bin door (4), the bin door (4) is rotatably connected with the device main body (1), the lower end of the bin door (4) is provided with a handle groove (6), the bin door (4) is provided with a visual window (5), and the visual window (5) and the bin door (4) are arranged into an integral structure.

5. The fluorescence signal detection device of the real-time fluorescence quantitative PCR instrument according to claim 3, characterized in that: put thing board (8) and device main part (1) sliding connection, the below of putting thing board (8) is provided with electric telescopic handle (29), and electric telescopic handle (29) are provided with two, and two electric telescopic handle (29) set up at the both ends of putting thing board (8), electric telescopic handle (29) and device main part (1) and put thing board (8) fixed connection.

6. The fluorescence signal detection device of the real-time fluorescence quantitative PCR instrument according to claim 1, characterized in that: the upper end of first T type mounting bracket (13) is provided with guide way (27), the top of first T type mounting bracket (13) is provided with guide bar (28), and guide bar (28) and device main part (1) fixed connection, guide bar (28) and first T type mounting bracket (13) sliding connection.