CN218404184U - A filtration separator for extracellular vesicle - Google Patents
A filtration separator for extracellular vesicle Download PDFInfo
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- CN218404184U CN218404184U CN202222822786.8U CN202222822786U CN218404184U CN 218404184 U CN218404184 U CN 218404184U CN 202222822786 U CN202222822786 U CN 202222822786U CN 218404184 U CN218404184 U CN 218404184U
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- extracellular vesicles
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Abstract
The utility model provides a filtering separation device for extracellular vesicles relates to extracellular vesicles separation technical field. The device comprises a filtering separation chamber and a collection chamber, wherein a plurality of filter membranes are vertically arranged in the filtering separation chamber at intervals, two sides of each filter membrane are connected with the side wall of the filtering separation chamber, and the bottom of each filter membrane is hinged with a baffle; the top of the filtering and separating chamber is provided with a sample inlet and a liquid inlet, the sample inlet is communicated with the inside of the liquid inlet through a control valve, and the lower part of one side of the filtering and separating chamber, which is far away from the sample inlet, is provided with a sample outlet; the filtering separation chamber is separated from the collection chamber through the sliding plate, the sliding plate is arranged in the chamber in a sliding mode, the height of the baffle is the same as the thickness of the sliding plate, the collection chamber comprises a plurality of collection cavities, and the collection cavities correspond to the bottoms of the filter membranes one to one in the distance. This application has the multiple filtration separation function, can carry out the multiple filtration separation to extracellular vesicle to wash it cleanly, obtain the material of different particle sizes, the separation effect is good, and separation efficiency is high.
Description
Technical Field
The utility model relates to an extracellular vesicle separation technical field particularly, relates to a filtering separation device for extracellular vesicle.
Background
Extracellular Vesicles (EV) refer to vesicular bodies of a double-layer membrane structure that are shed from the cell membrane or secreted from the cell, and have a diameter of between 50nm and 2 mm. The extracellular vesicles widely and stably exist in various body fluids, such as peripheral blood, urine, saliva, cerebrospinal fluid, milk, ascites, amniotic fluid and other body fluids, and carry various biomolecules (including protein, mRNA, miRNA and the like) from cells, and are important tools for carrying out substance transportation, signal transduction and realizing physiological functions of cells. EVs are largely divided into three major classes, depending on their biological origin: exosomes, microvesicles, and apoptotic bodies. Wherein the Exosomes (Exosomes) are extracellular vesicles having a diameter of about 40-150 nm.
The separation and purification of extracellular vesicles are usually achieved by ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or kits. For example, CN210856130U discloses a manual extracellular vesicle separation system, which employs a chromatography column packed with porous particle packing to filter liquid. CN108865971A discloses a method and apparatus for the separation of exosomes using porous anodic alumina membranes.
Some separation devices in the prior art need manual pressing, are time-consuming and labor-consuming and have low efficiency, and some separation devices only adopt a primary film for separation, so that the separation effect is poor; in addition, particulate matter such as extracellular vesicles often adheres to the filter or membrane in the separation device after use. However, the above extracellular vesicle separation device is difficult to clean the filter or the membrane and collect the particulate matter therein, which is not favorable for the reuse of the filter.
In view of the deficiencies of the prior art, it would be desirable to provide a filtration and separation device for extracellular vesicles that addresses the problems set forth in the background above.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a filtering separation device for extracellular vesicles, it can be to the weak point among the prior art, proposes the solution, and the function of multistage filtration separation and washing filter membrane can improve filtering separation effect and efficiency to can cyclic reuse the device, resources are saved.
The embodiment of the utility model provides a filtration and separation device for extracellular vesicles, which comprises a device body, wherein the device body comprises a filtration and separation chamber and a collection chamber, a plurality of filter membranes are arranged in the filtration and separation chamber at vertical intervals, two sides of each filter membrane are connected with the side wall of the filtration and separation chamber, and the bottom of each filter membrane is hinged with a baffle; the top of the filtering and separating chamber is provided with a sample inlet and a liquid inlet, the sample inlet is arranged close to one side of the top of the filtering and separating chamber, the liquid inlet is arranged close to the center of the top of the filtering and separating chamber, the sample inlet is communicated with the inside of the liquid inlet through a control valve, the lower part of one side of the filtering and separating chamber, which is far away from the sample inlet, is provided with a sample outlet, and the sample outlet faces the filtering surface of the filtering membrane;
the filtering and separating chamber and the collecting chamber are separated through a sliding plate, two sides of the sliding plate are in sliding connection with the side wall of the filtering and separating chamber, the height of the baffle is the same as the thickness of the sliding plate, the collecting chamber comprises a plurality of collecting cavities, and the collecting cavities are in one-to-one correspondence with the intervals between the bottoms of the filter membranes.
In some embodiments of the present invention, the filter membrane is disposed in parallel and inclined manner, wherein the opening of the filter membrane near the sample inlet and the top of the filtration separation chamber is larger than the opening area of the bottom of the filtration separation chamber.
In some embodiments of the present invention, the distance between the plurality of filter membranes gradually decreases from one side of the sample inlet to the opposite side thereof.
In some embodiments of the present invention, the pore size of the plurality of filter membranes decreases gradually from one side of the sample inlet to the opposite side thereof.
In some embodiments of the present invention, the filter membrane is a porous anodic aluminum oxide film.
In some embodiments of the present invention, the sample inlet and the inlet are respectively connected to a booster pump externally, and the sample outlet is connected to a negative pressure pump externally.
In some embodiments of the present invention, the upper surface of the slide plate is covered with a rubber layer.
In some embodiments of the present invention, the filtration separation chamber is located on both sides of the bottom of the filter membrane and is provided with a slide rail, the slide plate is slidably connected to the slide rail on both sides, the end of the slide rail is provided with a first magnetic member, and the slide plate is provided with a second magnetic member with opposite magnetism on one end in contact with the end of the slide rail.
In some embodiments of the present invention, the collection chamber is drawn in the collection chamber.
Compared with the prior art, the embodiment of the utility model has following advantage or beneficial effect at least:
the utility model comprises a device body, the device body comprises a filtering separation chamber and a collecting chamber, a plurality of filter membranes are arranged in the filtering separation chamber at vertical intervals, two sides of the filter membranes are connected with the side wall of the filtering separation chamber, and the bottom of each filter membrane is hinged with a baffle; the top of the filtering and separating chamber is provided with a sample inlet and a liquid inlet, the sample inlet is arranged close to one side of the top of the filtering and separating chamber, the liquid inlet is arranged close to the center of the top of the filtering and separating chamber, the sample inlet is communicated with the inside of the liquid inlet through a control valve, the lower part of one side of the filtering and separating chamber, which is far away from the sample inlet, is provided with a sample outlet, and the sample outlet faces to the filtering surface of the filter membrane; the filtering and separating chamber and the collecting chamber are separated through a sliding plate, two sides of the sliding plate are in sliding connection with the side wall of the filtering and separating chamber, the height of the baffle is the same as the thickness of the sliding plate, the collecting chamber comprises a plurality of collecting cavities, and the collecting cavities are in one-to-one correspondence with the intervals between the bottoms of the filter membranes. The cell outer vesicles entering from the sample inlet are subjected to multistage filtration and separation through a plurality of filter membranes arranged at intervals, the cell outer vesicles are subjected to coarse filtration and fine filtration in sequence, separation and purification of the cell outer vesicles with different particle sizes are realized, the sample inlet is arranged on one side of the top of the filtration and separation chamber, and the sample outlet is arranged on the opposite side of the filtration and separation chamber, so that the cell outer vesicles entering from the sample inlet can sequentially pass through the multistage filter membranes and are finally discharged from the sample outlet; introducing a balance liquid into the filtering and separating chamber through the liquid inlet, washing and cleaning the filtering and separating chamber and the filter membrane, wherein the balance liquid enters from the liquid inlet, flows from the inside of the filtering and separating chamber and then is discharged into different collecting cavities from the bottom under the action of a booster pump, and a plurality of separated particles with different particle sizes are collected; this application has the multiple filtration separation function, can carry out the multiple filtration separation to extracellular vesicle to wash it cleanly, obtain the material of different particle sizes, the separation effect is good, and separation efficiency is high.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic diagram of the internal structure of a filtration separation apparatus for extracellular vesicles according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a filtration and separation device for extracellular vesicles according to an embodiment of the present invention;
FIG. 3 is a schematic view of a connection structure between a slide plate and a slide rail according to an embodiment of the present invention;
fig. 4 is a top view of a filtration separation apparatus for extracellular vesicles, according to an embodiment of the present invention.
Reference numerals: 1. a filtration separation chamber; 2. a collection chamber; 3. a collection chamber; 4. filtering the membrane; 5. a baffle plate; 6. a sample inlet; 7. a liquid inlet; 8. a control valve; 9. a sample outlet; 10. a slide plate; 11. a slide rail; 12. a first magnetic member; 13. a handle.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without making creative efforts belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the embodiments of the present invention, it should be noted that, if the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, the description is only for convenience of description of the present invention and simplification, but the indication or suggestion that the device or element to be referred must have a specific position, be constructed and operated in a specific position, and therefore, the present invention should not be construed as being limited thereto.
In the description of the embodiments of the present invention, it should be further noted that unless otherwise explicitly stated or limited, the terms "set", "mounted", "connected" and "connected" should be interpreted broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
Examples
Referring to fig. 1 to 4, fig. 1 is a schematic diagram illustrating an internal structure of a filtration and separation apparatus for extracellular vesicles according to an embodiment of the present invention; FIG. 2 is a schematic structural view of a filtration and separation device for extracellular vesicles according to an embodiment of the present invention; FIG. 3 is a schematic view of a connection structure of the slide plate and the slide rail according to an embodiment of the present invention; FIG. 4 is a top view of a filtration separation apparatus for extracellular vesicles, according to an embodiment of the present invention;
a filtering and separating device for extracellular vesicles comprises: the device comprises a device body, wherein the device body comprises a filtering separation chamber 1 and a collection chamber 2, a plurality of filter membranes 4 are vertically arranged in the filtering separation chamber 1 at intervals, two sides of each filter membrane 4 are connected with the side wall of the filtering separation chamber 1, and the bottom of each filter membrane 4 is hinged with a baffle 5; the top of the filtering and separating chamber 1 is provided with a sample inlet 6 and a liquid inlet 7, the sample inlet 6 is arranged close to one side of the top of the filtering and separating chamber 1, the liquid inlet 7 is arranged close to the center of the top of the filtering and separating chamber 1, the sample inlet 6 is communicated with the inside of the liquid inlet 7 through a control valve 8, the lower part of one side of the filtering and separating chamber 1, which is far away from the sample inlet 6, is provided with a sample outlet 9, and the sample outlet 9 faces the filtering surface of the filter membrane 4;
The utility model comprises a device body, the device body comprises a filtering separation chamber 1 and a collecting chamber 2, a plurality of filter membranes 4 are vertically arranged in the filtering separation chamber 1 at intervals, two sides of the filter membranes 4 are connected with the side wall of the filtering separation chamber 1, and the bottom of each filter membrane 4 is hinged with a baffle 5; the top of the filtering and separating chamber 1 is provided with a sample inlet 6 and a liquid inlet 7, the sample inlet 6 is arranged close to one side of the top of the filtering and separating chamber 1, the liquid inlet 7 is arranged close to the center of the top of the filtering and separating chamber 1, the sample inlet 6 is communicated with the inside of the liquid inlet 7 through a control valve 8, the lower part of one side of the filtering and separating chamber 1, which is far away from the sample inlet 6, is provided with a sample outlet 9, and the sample outlet 9 faces the filtering surface of the filter membrane 4; filtration separation chamber 1 and collection room 2 pass through slide 10 to separate, and the both sides of slide 10 and the lateral wall sliding connection of filtration separation chamber 1, and wherein, the height of baffle 5 is the same with slide 10's thickness, collects room 2 and includes a plurality of collection chambeies 3, and a plurality of collection chambeies 3 and the interval one-to-one between a plurality of filter membrane 4 bottoms. The cell outer vesicles entering from the sample inlet 6 are subjected to multistage filtration and separation through a plurality of filter membranes 4 arranged at intervals, the cell outer vesicles are subjected to coarse filtration and fine filtration in sequence, separation and purification of the cell outer vesicles with different particle sizes are realized, the sample inlet 6 is arranged on one side of the top of the filtration and separation chamber 1, and the sample outlet 9 is arranged on the opposite side of the filtration and separation chamber, so that the cell outer vesicles entering from the sample inlet 6 can pass through the multistage filter membranes 4 in sequence and are finally discharged from the sample outlet 9; introducing balance liquid into the filtering and separating chamber 1 through the liquid inlet 7, washing and cleaning the filtering and separating chamber 1 and the filter membrane 4, introducing the balance liquid from the liquid inlet 7, discharging the balance liquid into different collecting cavities 3 from the bottom after the balance liquid flows from the interior of the filtering and separating chamber under the action of a booster pump, and collecting multiple separated particles with different particle sizes; this application has the multiple filtration separation function, can carry out the multiple filtration separation to extracellular vesicle to wash it cleanly, obtain the material of different particle sizes, the separation effect is good, and separation efficiency is high.
Next, a filtration separation apparatus for extracellular vesicles of the present exemplary embodiment will be further described.
In one embodiment of this embodiment, the device body includes a filtration separation chamber 1 and a collection chamber 2, a plurality of filter membranes 4 are vertically disposed in the filtration separation chamber 1 at intervals, two sides of each filter membrane 4 are connected to a side wall of the filtration separation chamber 1, and a baffle 5 is hinged to the bottom of each filter membrane 4; the top of the filtering and separating chamber 1 is provided with a sample inlet 6 and a liquid inlet 7, the sample inlet 6 is arranged close to one side of the top of the filtering and separating chamber 1, the lower part of one side of the filtering and separating chamber 1, which is far away from the sample inlet 6, is provided with a sample outlet 9, the sample outlet 9 faces the filtering surface of the filter membrane 4, so that extracellular vesicles enter from the sample inlet 6 and can be sequentially filtered and separated through the filter membranes 4 and finally discharged from the sample outlet 9, the liquid inlet 7 is conical and is arranged close to the center of the top of the filtering and separating chamber 1, the sample inlet 6 is communicated with the inside of the liquid inlet 7 through a control valve 8, the liquid inlet 7 is filled with balance liquid, the control valve 8 is opened, the balance liquid respectively enters between the gaps of each filter membrane 4, the filter membranes 4 are washed, and particles attached to the filter membranes 4 are washed downwards and fall into the collecting chamber 2; wherein, above-mentioned introduction port 6 and inlet 7 are external booster pump respectively, and above-mentioned out sample port 9 is external negative pressure pump, under the effect of booster pump and negative pressure pump, make the flow direction of the outer vesicle of cell towards out sample port 9 one side during the filtration separation, make it filter many times, and the accessible is adjusted pressure simultaneously and is changed the pressure environment in the filtration separation chamber 1, reaches the best separation effect.
In an embodiment of this embodiment, the filtering and separating chamber 1 and the collecting chamber 2 are separated by a sliding plate 10, two sides of the sliding plate 10 are slidably connected with side walls of the filtering and separating chamber 1, wherein the height of the baffle plate 5 is the same as the thickness of the sliding plate 10, when filtering and separating are performed, the sliding plate 10 slides into the chamber, the hinged baffle plate 5 is turned over upwards, the filtered and separated liquid cannot flow downwards into the collecting chamber 2, after filtering is completed, the sliding plate 10 is drawn out, the baffle plate 5 is automatically connected in a drooping manner, the collecting chamber 2 comprises a plurality of collecting chambers 3, the plurality of collecting chambers 3 correspond to the bottoms of the plurality of filter membranes 4 one by one, and after the baffle plate 5 drooping, each collecting chamber 3 is separated, so that separated particles on each filter membrane 4 can accurately fall into the corresponding collecting chamber 3.
In a particular embodiment, the collection chamber 3 is drawn inside the collection chamber 2, facilitating the removal of the collected particles.
In one embodiment, the upper surface of the slider 10 is coated with a rubber layer. The sealing performance between the sliding plate 10 and the filtering separation chamber 1 can be improved, and the pressure environment in the filtering separation chamber 1 is ensured to be in a stable state when filtering separation is carried out; the outer end of the slider 10 is provided with a handle 13 for facilitating the sliding of the slider 10.
In an embodiment, the two sides of the filtration separation chamber 1, which are located at the bottom of the filtration membrane 4, are provided with slide rails 11, the two sides of the slide plate 10 are slidably connected with the slide rails 11, the end of the slide rail 11 is provided with a first magnetic member 12, and the end of the slide plate 10, which is in contact with the end of the slide rail 11, is provided with a second magnetic member with opposite magnetism. When the slide board 10 is in a sealed state, the connection stability between the slide board 10 and the slide rail 11 is ensured, the slide board 10 is prevented from sliding outwards due to careless touch, meanwhile, a gap is prevented from being generated between the end parts of the slide board 10 and the slide rail 11, and when the slide board 10 needs to be pulled out, a certain external force is applied to the handle 13, so that the slide board 10 can be pulled out.
In one embodiment of this embodiment, the filter membrane 4 is disposed in parallel and inclined manner, wherein the opening of the filter membrane 4 near the sample inlet 6 and the top of the filtration and separation chamber 1 is larger than the opening area of the bottom thereof. The filter membrane 4 of slope parallel arrangement can avoid some extracellular vesicles direct from the top down to flow and not pass through filter membrane 4, combines the filter membrane 4 of slope under the effect of booster pump and negative pressure pump, is the negative pressure in the filtration separation chamber 1, flows towards outlet 9 one side and filters the separation.
As an example, the distance between the plurality of filter membranes 4 gradually decreases from one side of the injection port 6 to the opposite side thereof. The filter membrane 4 that is located the front side carries out prefiltration to the extracellular vesicle, has a large amount of liquid and granule during initial filtration, and the interval is great is convenient for hold and filter, and follow-up granule reduces gradually, reduces the interval and can improve the effect of filtering separation, avoids its vertical drop between great interval.
As an example, the pore size of the plurality of filter membranes 4 gradually decreases from one side of the injection port 6 to the opposite side thereof. Specifically, the filter membrane 4 may be a porous anodic alumina membrane, and has a fine pore size and a good filtering and separating effect.
The working principle is as follows: when needing to carry out the filtration separation, with the external booster pump of inlet 6, with the external negative pressure pump of appearance mouth 9, close inlet 7 and slide 10, let in the appearance vesicle from the outside in inlet 6 gets into filtration separation chamber 1, under the effect of negative pressure, the appearance vesicle liquid of cell filters the separation through a plurality of filter membranes 4 in proper order, attach to the filter membrane 4 of different aperture sizes with the granule of different particle sizes, liquid is discharged from appearance mouth 9, filter the back, about inlet 6 and appearance mouth 9, open inlet 7 and control valve 8 (control valve 8 can be by setting up in the outside switch board control of device), pull out slide 10, 5 automatic vertical whereabouts of baffle this moment and every lateral wall butt of collecting chamber 3, inlet 7 external booster pump, let in inlet 7 from the outside and get into filtration separation chamber 1 in with the balance liquid and wash filter membrane 4, will attach to granule wash down on filter membrane 4 and fall into in the collection chamber 3 of difference, thereby realize the filtration and the separation of the appearance vesicle of cell.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The filtering and separating device for the extracellular vesicles is characterized by comprising a device body, wherein the device body comprises a filtering and separating chamber and a collecting chamber, a plurality of filter membranes are vertically arranged in the filtering and separating chamber at intervals, two sides of each filter membrane are connected with the side wall of the filtering and separating chamber, and the bottom of each filter membrane is hinged with a baffle; the top of the filtering and separating chamber is provided with a sample inlet and a liquid inlet, the sample inlet is arranged close to one side of the top of the filtering and separating chamber, the liquid inlet is arranged close to the center of the top of the filtering and separating chamber, the sample inlet is communicated with the inside of the liquid inlet through a control valve, the lower part of one side of the filtering and separating chamber, which is far away from the sample inlet, is provided with a sample outlet, and the sample outlet faces to the filtering surface of the filter membrane;
the filtering and separating chamber and the collecting chamber are separated through a sliding plate, two sides of the sliding plate are in sliding connection with the side wall of the filtering and separating chamber, the height of the baffle is the same as the thickness of the sliding plate, the collecting chamber comprises a plurality of collecting cavities, and the collecting cavities are in one-to-one correspondence with the intervals between the bottoms of the filter membranes.
2. The apparatus according to claim 1, wherein the filter membranes are disposed in parallel and inclined manner, and wherein the openings of the filter membranes near the injection port and the top of the filter separation chamber have a larger area than the openings at the bottom thereof.
3. The filter separation device for extracellular vesicles according to claim 1, wherein a distance between the plurality of filter membranes gradually decreases from one side of the injection port to an opposite side thereof.
4. The filtration separation device for extracellular vesicles of claim 1, wherein pore sizes of the plurality of filtration membranes are gradually reduced from one side of the sample inlet to an opposite side thereof.
5. The filtering separation device for extracellular vesicles according to claim 1, wherein the filter membrane is a porous anodic alumina membrane.
6. The filtering and separating device for extracellular vesicles according to claim 1, wherein the sample inlet and the liquid inlet are respectively connected with a booster pump, and the sample outlet is connected with a negative pressure pump.
7. The filtering and separating device for extracellular vesicles according to claim 1, wherein the upper surface of the sliding plate is coated with a rubber layer.
8. The device for filtering and separating extracellular vesicles as claimed in claim 1, wherein the filtering and separating chamber is provided with slide rails on two sides of the bottom of the filter membrane, the slide rails are slidably connected to two sides of the slide rail, a first magnetic member is provided at an end of the slide rail, and a second magnetic member with opposite magnetism is provided at an end of the slide rail contacting with the end of the slide rail.
9. The filtering separation device for extracellular vesicles according to claim 1, wherein the outer end of the slide plate is provided with a handle.
10. The filtered separation device for extracellular vesicles of claim 1, wherein the collection chamber is drawn within the collection chamber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222822786.8U CN218404184U (en) | 2022-10-25 | 2022-10-25 | A filtration separator for extracellular vesicle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222822786.8U CN218404184U (en) | 2022-10-25 | 2022-10-25 | A filtration separator for extracellular vesicle |
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| Publication Number | Publication Date |
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| CN218404184U true CN218404184U (en) | 2023-01-31 |
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| CN202222822786.8U Active CN218404184U (en) | 2022-10-25 | 2022-10-25 | A filtration separator for extracellular vesicle |
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