CN218358540U

CN218358540U - Device is prepared to colored prestained protein molecular weight standard reagent with get function of volume

Info

Publication number: CN218358540U
Application number: CN202222165975.2U
Authority: CN
Inventors: 何秀琴
Original assignee: Nanjing Hemiao Biotechnology Co ltd
Current assignee: Nanjing Hemiao Biotechnology Co ltd
Priority date: 2022-08-17
Filing date: 2022-08-17
Publication date: 2023-01-24
Anticipated expiration: 2032-08-17

Abstract

The utility model discloses a colored prestained protein molecular weight standard reagent preparation device with volume function, belong to reagent preparation device field, including preparing the subassembly, the volume is got the structure including setting up backup pad on preparing the staving outer wall and the storage case of setting in the backup pad upper end, the upper end of storage case is provided with the volume and is got a section of thick bamboo, the lower extreme of getting a section of thick bamboo is installed the volume and is got the pipe, and the volume is got the lower extreme and is linked together with the storage case, still be provided with row material pipe on getting the lateral wall of a section of thick bamboo, the inside of getting a section of thick bamboo is provided with the lifting rod, the lower extreme of lifting rod is provided with the piston, the surface of getting a section of thick bamboo evenly is provided with the scale mark, handheld lifting rod, and upwards stimulate, utilize the volume of setting to get the pipe this moment and can extract the raw materials to get a section of thick bamboo in, close valve B, and open valve C, downwards press the lifting rod, utilize the piston can pour into the raw materials into the preparation staving from row material pipe, and utilize the scale mark of setting to measure, thereby can realize the purpose of getting the raw materials.

Description

Device for preparing color pre-dyed protein molecular weight standard reagent with measuring function

Technical Field

The utility model relates to a device is prepared to reagent, and more specifically says, relates to device is prepared to colored prestained protein molecular weight standard reagent with get function.

Background

The pre-dyed protein is a mixture consisting of 10 blue-dyed, orange-dyed and green-dyed recombinant proteins (10-170 kDa), is used as a standard substance for measuring the molecular weight in protein electrophoresis, can directly observe the protein electrophoresis and clearly judge the transfer effect, the molecular weight of the pre-dyed protein aims at monitoring the electrophoresis process, evaluating the transfer rate transferred to PVDF, nylon and nitrocellulose membranes, and estimating the approximate molecular weight of visible separated proteins after gel dyeing or using a detection reagent, and reagent raw materials need to be stirred and prepared by a stirring barrel in the preparation process of the color pre-dyed protein molecular weight standard reagent.

When the existing color pre-dyeing protein molecular weight standard reagent is used in a preparation device, a reagent raw material is required to be poured into a preparation barrel firstly, and the reagent raw material is mixed by using a stirring device, but in the preparation process, the input amount of the reagent raw material is inconvenient to measure, so that the color pre-dyeing protein molecular weight standard reagent has inaccurate precision, influences normal use and has unsatisfactory use effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the in-process of preparing among the prior art, the inconvenient volume of getting of input of reagent raw materials leads to colored prestained protein molecular weight standard reagent precision inaccurate, influences normal use, and the result of use is unsatisfactory, provides the colored prestained protein molecular weight standard reagent preparation device that has the function of getting the volume to it is not enough more than solving, facilitates the use.

In order to achieve the above purpose, the utility model provides a technical scheme does:

the utility model discloses a colored prestained protein molecular weight standard reagent preparation device with volume is got function, including preparing the subassembly, prepare the subassembly including preparing the staving and setting up the sealed apron in preparing the staving upper end, the upper end of sealed apron is provided with the stirring structure, the outside of preparing the staving is provided with support piece, still installs mounting structure and volume structure on the lateral wall of preparing the staving, and mounting structure is in one side of volume structure;

the measuring structure comprises a supporting plate and a storage box, the supporting plate is arranged on the outer wall of the barrel body, the storage box is arranged at the upper end of the supporting plate, a measuring cylinder is arranged at the upper end of the storage box, a measuring pipe is mounted at the lower end of the measuring cylinder, the lower end of the measuring pipe is communicated with the storage box, a discharging pipe is further arranged on the side wall of the measuring cylinder, a lifting rod is arranged inside the measuring cylinder, a piston is arranged at the lower end of the lifting rod, the piston is located inside the measuring cylinder, and scale marks are uniformly arranged on the surface of the measuring cylinder.

Preferably, the surface activity of storage case is provided with the dodge gate, and the surface of storage case still is provided with the connector, and the volume is got the pipe and is passed the connector to extend to the inside of storage case.

Preferably, the valve B is arranged on the outer side of the measuring pipe, and the valve C is arranged on the outer side of the discharging pipe.

Preferably, the lower end of the preparation barrel body is provided with a discharging pipe, the outer side of the discharging pipe is provided with a valve A, and one side of the sealing cover plate, which is close to the preparation barrel body, is provided with a non-stick layer.

Preferably, the support piece comprises an external ring arranged outside the preparation barrel body and a support rod arranged at the lower end of the external ring, and the lower end of the support rod is connected with the bottom block.

Preferably, the bracing piece is provided with three groups, three groups the bracing piece all the slope set up the lower extreme at external ring.

Preferably, the stirring structure comprises a motor base and a motor arranged at the upper end of the motor base, and the output end of the motor penetrates through the motor base and the sealing cover plate and is connected with the spindle rod.

Preferably, the lantern ring has evenly been cup jointed in the outside of main shaft pole, and the lantern ring is provided with four groups, and one side of the lantern ring of three groups is provided with the puddler, and the outside of another group lantern ring is provided with scrapes the wall pole.

Preferably, the mounting structure comprises a fixing plate arranged on the outer wall of the preparation barrel body and side grooves formed in the side walls of the fixing plate, the inside of each side groove is connected with a clamping plate in a sliding mode, and the clamping plates are provided with two groups.

Preferably, threaded holes are uniformly distributed in the surface of the fixing plate, the side groove and the clamping plate are fixed through bolts, and an anti-skid layer is arranged on the inner wall of one side, close to the measuring cylinder, of the clamping plate.

Adopt the technical scheme provided by the utility model, compare with prior art, have following beneficial effect:

compared with the prior art, the beneficial effects of the utility model are as follows:

(1) The utility model discloses a colored prestained protein molecular weight standard reagent preparation device with volume function, get pipe, connector, storage case, the handle bar, volume section of thick bamboo and piston through the volume that sets up, and the storage incasement prestores the oil raw materials, close valve C, then hand the handle bar, and upwards stimulate, utilize the volume of setting to get the pipe at this moment and can extract the raw materials to the volume to get in the section of thick bamboo, when extracting a volume, close valve B, and open valve C, press the handle bar downwards, utilize the piston can pour into the raw materials into the preparation bucket from arranging the material pipe, and utilize the scale mark that sets up to measure the raw materials, thereby can realize the purpose of volume raw materials, then close valve C once more, open motor work, impel the main shaft pole, the lantern ring, the puddler and scrape the wall pole and rotate, can stir the raw materials and mix, can accomplish the preparation to the reagent;

(2) The utility model discloses a device is prepared to colored prestained protein molecular weight standard reagent with volume function of getting, fixed plate through setting up, the side channel, the pinch-off blades, screw hole and bolt, a section of thick bamboo is got to handheld volume, and be in one side of fixed plate with it, two sets of pinch-off blades of activity this moment, and get the outer wall of a section of thick bamboo with its being close to the volume, screw hole and bolt through setting up can fix the position of pinch-off blades, can realize getting the fixed of a section of thick bamboo to the volume, so that follow-up volume is got the use, it gets a section of thick bamboo to need not artifical handheld volume that lasts, and one side that the pinch-off blades is close to the volume and gets a section of thick bamboo is provided with the skid resistant course, can effectively play anti-skidding effect.

Drawings

FIG. 1 is an overall structure diagram of the present invention;

FIG. 2 is a schematic view of the supporting member of the present invention;

FIG. 3 is a schematic view of the stirring structure of the present invention;

fig. 4 is a partial schematic view of the measuring structure of the present invention;

FIG. 5 is a partial schematic view of the installation structure of the present invention;

fig. 6 is an enlarged schematic view of a in fig. 1 according to the present invention.

In the figure: 1. preparing a component; 11. preparing a barrel body; 111. a feeding pipe; 112. a valve A; 12. sealing the cover plate; 121. a non-stick layer; 13. a stirring structure; 131. a motor base; 132. a motor; 133. a main shaft rod; 134. a collar; 135. a stirring rod; 136. a wall scraping rod; 14. a support member; 141. an external ring; 142. a support bar; 143. a bottom block; 15. measuring a structure; 151. a support plate; 152. a material storage box; 1521. a movable door; 1522. a connecting port; 153. measuring a cylinder; 1531. scale lines; 154. measuring a pipe; 155. lifting a pull rod; 156. a piston; 157. a discharge pipe; 158. a valve B; 159. a valve C; 16. a mounting structure; 161. a fixing plate; 162. a side groove; 163. a clamping plate; 164. a threaded hole; 165. a bolt; 166. and an anti-slip layer.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

For a further understanding of the present invention, reference will be made to the following detailed description taken in conjunction with the accompanying drawings.

Combine fig. 1, the utility model discloses a device is prepared to colored prestained protein molecular weight standard reagent with function is got to volume, including preparing subassembly 1, prepare subassembly 1 including preparing staving 11 and setting up at the sealed apron 12 of preparing staving 11 upper end, the upper end of sealed apron 12 is provided with stirring structure 13, and the outside of preparing staving 11 is provided with support piece 14, still installs mounting structure 16 on the lateral wall of preparing staving 11 and gets structure 15 with the volume, and mounting structure 16 is in one side of getting structure 15 with the volume.

The present invention will be further described with reference to the following examples.

The first embodiment is as follows:

with reference to fig. 2 to 4, the measuring structure 15 includes a supporting plate 151 disposed on the outer wall of the dispensing barrel 11 and a material storage box 152 disposed on the upper end of the supporting plate 151, a measuring cylinder 153 is disposed on the upper end of the material storage box 152, a measuring pipe 154 is disposed at the lower end of the measuring cylinder 153, the lower end of the measuring pipe 154 is communicated with the material storage box 152, a discharging pipe 157 is disposed on the sidewall of the measuring cylinder 153, a lifting rod 155 is disposed inside the measuring cylinder 153, a piston 156 is disposed at the lower end of the lifting rod 155, the piston 156 is located inside the measuring cylinder 153, scale lines 1531 are uniformly disposed on the surface of the measuring cylinder 153, and the setting of the scale lines 1531 can display the liquid amount of the extracted reagent raw material.

The surface of the storage box 152 is movably provided with a movable door 1521, the surface of the storage box 152 is further provided with a connecting port 1522, and the measuring pipe 154 passes through the connecting port 1522 and extends into the storage box 152.

A valve B158 is provided outside the measuring pipe 154, and a valve C159 is provided outside the discharging pipe 157.

A blanking pipe 111 is arranged at the lower end of the preparation barrel body 11, a valve a112 is arranged at the outer side of the blanking pipe 111, and a non-stick layer 121 is arranged at one side of the sealing cover plate 12 close to the preparation barrel body 11, so that a reagent is prevented from being stained on the inner wall of the sealing cover plate 12.

The supporting member 14 includes an external ring 141 disposed outside the preparation barrel body 11 and a supporting rod 142 disposed at a lower end of the external ring 141, a lower end of the supporting rod 142 is connected to the bottom block 143, and the preparation barrel body 11 can be supported by the supporting rod 142 and the bottom block 143.

The supporting rods 142 are provided with three groups, and the three groups of supporting rods 142 are all obliquely arranged at the lower end of the external ring 141.

The stirring structure 13 includes a motor base 131 and a motor 132 disposed at an upper end of the motor base 131, and an output end of the motor 132 penetrates through the motor base 131 and the sealing cover 12 and is connected to the spindle rod 133.

Lantern rings 134 are evenly sleeved on the outer sides of the main shaft rods 133, four groups of lantern rings 134 are arranged, a stirring rod 135 is arranged on one side of each of the three groups of lantern rings 134, a wall scraping rod 136 is arranged on the outer side of the other group of lantern rings 134, the motor 132 is started to work, the main shaft rods 133, the lantern rings 134, the stirring rods 135 and the wall scraping rod 136 are made to rotate, and raw materials can be stirred and mixed.

In this embodiment, during the use, the operator will get pipe 154 and pass connector 1522, and extend to the inside of storage tank 152, and the inside oil raw materials that stores in advance of storage tank 152, close valve C159, and then hand pull rod 155, and upwards stimulate, this moment utilize the pipe 154 of getting of setting to extract the raw materials to get in the section of thick bamboo 153, when extracting a ration, close valve B158, and open valve C159, press pull rod 155 downwards, utilize piston 156 can pour the raw materials into preparation staving 11 from row material pipe 157, and utilize the scale mark 1531 that sets up to measure the raw materials, thereby can realize getting the purpose of getting the raw materials, then close valve C159 once more, open motor 132 work, impel main shaft 133, lantern ring 134, puddler 135 and scrape wall bar 136 and rotate, can stir the raw materials and mix, can accomplish the preparation to the reagent.

Example two:

with reference to fig. 5 and 6, the mounting structure 16 includes a fixing plate 161 disposed on the outer wall of the dispensing barrel 11 and side grooves 162 disposed on the side walls of the fixing plate 161, two sets of clamping plates 163 are slidably connected to the inside of the side grooves 162, and the clamping plates 163 are disposed, and the clamping plates 163 are used for clamping the measuring cylinder 153.

Threaded holes 164 are uniformly distributed in the surface of the fixing plate 161, the side groove 162 and the clamping plate 163 are fixed through bolts 165, and an anti-slip layer 166 is arranged on the inner wall of one side, close to the measuring cylinder 153, of the clamping plate 163.

In this embodiment, during the use, the handheld section of thick bamboo 153 of getting of operating personnel, and with its one side that is in fixed plate 161, two sets of clamp plate 163 of activity this moment, and with its outer wall of getting a section of thick bamboo 153 of getting close to, can fix the position of clamp plate 163 through the screw hole 164 and the bolt 165 that set up, can realize getting the fixed of a section of thick bamboo 153 of getting, so that the use is got to the follow-up volume, need not the manual work and continuously get a section of thick bamboo 153 of getting, and one side that clamp plate 163 is close to getting a section of thick bamboo 153 of getting is provided with skid resistant course 166, can effectively play anti-skidding effect.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. Colored prestained protein molecular weight standard reagent preparation device with volume function, including preparing subassembly (1), its characterized in that: the preparation assembly (1) comprises a preparation barrel body (11) and a sealing cover plate (12) arranged at the upper end of the preparation barrel body (11), a stirring structure (13) is arranged at the upper end of the sealing cover plate (12), a supporting piece (14) is arranged on the outer side of the preparation barrel body (11), a mounting structure (16) and a measuring structure (15) are further mounted on the side wall of the preparation barrel body (11), and the mounting structure (16) is positioned on one side of the measuring structure (15);

the measuring structure (15) comprises a supporting plate (151) arranged on the outer wall of the barrel body (11) and a material storage box (152) arranged at the upper end of the supporting plate (151), a measuring cylinder (153) is arranged at the upper end of the material storage box (152), a measuring pipe (154) is installed at the lower end of the measuring cylinder (153), the lower end of the measuring pipe (154) is communicated with the material storage box (152), a discharging pipe (157) is further arranged on the side wall of the measuring cylinder (153), a lifting rod (155) is arranged inside the measuring cylinder (153), a piston (156) is arranged at the lower end of the lifting rod (155), the piston (156) is located inside the measuring cylinder (153), and scale marks (1531) are uniformly arranged on the surface of the measuring cylinder (153).

2. The device for preparing a color prestained protein molecular weight standard reagent with measuring function according to claim 1, characterized in that: the surface activity of storage case (152) is provided with dodge gate (1521), and the surface of storage case (152) still is provided with connector (1522), and gets to manage (154) and pass connector (1522) to extend to the inside of storage case (152).

3. The device for preparing the color prestained protein molecular weight standard reagent with the measuring function according to claim 2, wherein: the outer side of the measuring pipe (154) is provided with a valve B (158), and the outer side of the discharging pipe (157) is provided with a valve C (159).

4. The device for preparing a color prestained protein molecular weight standard reagent with measuring function according to claim 3, characterized in that: the lower end of the preparation barrel body (11) is provided with a blanking pipe (111), the outer side of the blanking pipe (111) is provided with a valve A (112), and one side, close to the preparation barrel body (11), of the sealing cover plate (12) is provided with a non-stick layer (121).

5. The device for preparing a color prestained protein molecular weight standard reagent with measuring function according to claim 4, wherein: the supporting piece (14) comprises an external ring (141) arranged on the outer side of the preparation barrel body (11) and a supporting rod (142) arranged at the lower end of the external ring (141), and the lower end of the supporting rod (142) is connected with the bottom block (143).

6. The device for preparing a color prestained protein molecular weight standard reagent with measuring function according to claim 5, characterized in that: the supporting rods (142) are provided with three groups, and the three groups of supporting rods (142) are obliquely arranged at the lower end of the external ring (141).

7. The device for preparing the color prestained protein molecular weight standard reagent with measuring function according to claim 6, characterized in that: stirring structure (13) include motor cabinet (131) and set up motor (132) in motor cabinet (131) upper end, and motor cabinet (131) and sealed apron (12) are run through to the output of motor (132) to be connected with main shaft rod (133).

8. The device for preparing the color prestained protein molecular weight standard reagent with measuring function according to claim 7, characterized in that: lantern rings (134) are uniformly sleeved on the outer sides of the main shaft rods (133), four groups of lantern rings (134) are arranged, stirring rods (135) are arranged on one sides of the three groups of lantern rings (134), and wall scraping rods (136) are arranged on the outer sides of the other group of lantern rings (134).

9. The device for preparing a color prestained protein molecular weight standard reagent with measuring function according to claim 8, wherein: the mounting structure (16) comprises a fixing plate (161) arranged on the outer wall of the preparation barrel body (11) and side grooves (162) formed in the side wall of the fixing plate (161), clamping plates (163) are connected to the inner portions of the side grooves (162) in a sliding mode, and two sets of clamping plates (163) are arranged.

10. The device for preparing the color prestained protein molecular weight standard reagent with measuring function according to claim 9, wherein: threaded holes (164) are uniformly distributed in the surface of the fixing plate (161), the side groove (162) and the clamping plate (163) are fixed through bolts (165), and an anti-skid layer (166) is arranged on the inner wall of one side, close to the measuring cylinder (153), of the clamping plate (163).