CN218321393U - Portable water microorganism sampling culture and detection integrated device - Google Patents
Portable water microorganism sampling culture and detection integrated device Download PDFInfo
- Publication number
- CN218321393U CN218321393U CN202221989691.9U CN202221989691U CN218321393U CN 218321393 U CN218321393 U CN 218321393U CN 202221989691 U CN202221989691 U CN 202221989691U CN 218321393 U CN218321393 U CN 218321393U
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- Prior art keywords
- incubator
- filter
- box body
- microorganism
- sampling
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- 244000005700 microbiome Species 0.000 title claims abstract description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 238000005070 sampling Methods 0.000 title claims abstract description 37
- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 238000003860 storage Methods 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 5
- 241000220317 Rosa Species 0.000 claims abstract 2
- 238000001914 filtration Methods 0.000 claims description 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 229910052760 oxygen Inorganic materials 0.000 claims description 8
- 239000001301 oxygen Substances 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 230000007306 turnover Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000012085 test solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 241000590020 Achromobacter Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000191368 Chlorobi Species 0.000 description 1
- 241000588881 Chromobacterium Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000295146 Gallionellaceae Species 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- 241000192142 Proteobacteria Species 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses a portable water body microorganism sampling, culturing and detecting integrated device, which is provided with a filter box on the upper part of a box body, an incubator and a microorganism detection meter are arranged below the filter box, a culture liquid tank is arranged on the upper end of the box body, an input interface, a storage battery, a central processing unit, a control panel and a microorganism movable display screen are arranged on one side of the box body, a peristaltic pump and a telescopic rod are arranged on the other outer side surface of the box body, and one end of the telescopic rod is connected with a peristaltic pipe retractor; the below of peristaltic tube contractor install the motor contractor, the one end of peristaltic tube with rose box intercommunication, the other end and leaking hopper-shaped sampling head and being connected, the sampling head links to each other with the heavy object piece, the heavy object piece is fixed in motor contractor below through the messenger who is equipped with the scale. The utility model relates to a novelty, convenient operation can realize extracting, cultivateing and detecting water microorganism, collects multiple functions in an organic whole, can effectual improvement work efficiency, facilitate promotion.
Description
Technical Field
The utility model relates to a microorganism detection device, concretely relates to microorganism sample is cultivateed and is detected integrated device.
Background
The microorganisms in the water body are small in individuals and various in types, and widely relate to the fields of medicine, industry and agriculture, environmental protection and the like. The distribution of microorganisms in fresh water is affected by nutrients, temperature, dissolved oxygen, pH, and the like. The microorganisms in water are mainly autotrophic sulfur bacteria, iron bacteria and cocci, photosynthetic pigment-containing cyanobacteria, green sulfur bacteria and purple bacteria, and chromobacterium, achromobacter and micrococcus capable of growing in clear water with low nutrient content. A small amount of aquatic mold can grow on decaying organisms, and algae and some protozoa often grow on the water. These microorganisms are generally considered to be microorganisms in the clean water body, and are important to water quality. Plays an irreplaceable role in the nutrient salt and substance circulation of the aquatic ecosystem. In addition, microorganisms are extremely sensitive to the environment and are important environmental indicators. Therefore, the research and study of the microorganisms in the water body are of great significance to the water body environment monitoring, the aquatic ecosystem study and the like.
The traditional microbial ecology test device only uses a container to sample from water, and has the following disadvantages: 1. the water at different positions and depths has different microorganisms, so that the water at different positions and different depths cannot be sampled and detected; 2. the existing device does not have a portable device integrating sampling, culturing and detecting, and can not effectively culture and timely detect microorganisms. Inconvenient storage and transportation, and heavy and repetitive work is brought to scientific researchers; 3. collecting a water sample for microbial detection, wherein the water sample is easily polluted by the outside in the process of censorship and cannot reflect the real condition of local water body microbes at that time; 4. the detection device cannot be cleaned, and the detection accuracy cannot be guaranteed.
Disclosure of Invention
An object of the utility model is to provide a portable water microorganism sample is cultivateed and is detected integrated device can take a sample and carry out effectual cultivation and timely detection to the water microorganism of different positions and degree of depth.
In order to achieve the above purpose, the utility model discloses a portable water microorganism sample is cultivateed and is detected integrated device, its concrete technical scheme as follows: the device comprises a box body, wherein an ultraviolet lamp is installed at the top in the box body, a filter box is arranged at the upper part of the box body, the filter box is connected with an incubator below the filter box through a connecting pipe provided with a first turn-buckle filter valve, the incubator is connected with a microorganism detector below the incubator through a connecting pipe, an infrared flow detector and a water outlet pipe are arranged on the filter box, a second turn-buckle filter valve is arranged at the inlet end of the water outlet pipe, an oxygen pump, a heating rod and a temperature and humidity sensor are installed on the side wall of the incubator, a stirrer is installed at the bottom of the incubator, and a bacteria taking port, a far infrared camera and a micrometer are arranged on the microorganism detector; the upper end of the box body is provided with a culture liquid groove, the culture liquid groove is provided with a liquid feeding port, and the bottom of the culture liquid groove is communicated with the incubator through a pipe; one side of the box body is provided with an input interface, a storage battery, a central processing unit, a control panel and a microorganism movable display screen, the other outer side surface of the box body is provided with a peristaltic pump and a telescopic rod, and one end of the telescopic rod is connected with a peristaltic tube retractor; the below of peristaltic tube contractor install the motor contractor, the one end of peristaltic tube with filter intercommunication, the other end and leak hopper-shaped sampling head and be connected, the sampling head links to each other with the heavy object piece, the heavy object piece is fixed in motor contractor below through the messenger who is equipped with the scale.
The agitator including installing at incubator bottom agitator motor, the stirring rake passes through the wave form pivot and is connected with agitator motor's motor shaft.
And a collecting bottle is arranged in the box body.
The control panel is provided with a control key, a temperature display, an oxygen display and a culture solution display.
And the top of the incubator is provided with an LED lamp.
The first turnbuckle filter valve and the second turnbuckle filter valve are identical in structure and comprise a valve body and a filter disc in the valve body, one side of the filter disc is fixed with a rotating shaft, the rotating shaft penetrates out of the valve body to be connected with a handle, and a filter membrane and a filter screen are placed on the filter disc.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) The utility model discloses structural design is novel, and convenient operation can realize the sample to water microorganism, cultivates and detects, collects multiple functions in an organic whole, can direct suction filtration microorganism, also can cultivate and go on detecting the observation after well, can effectual improvement work efficiency, facilitate promotion.
(2) The utility model discloses in the manual filter screen of turning over of installation in the filter box, can turn over and detain down the microorganism, also can take off from the device, more convenient filtration microorganism, also the replacement filtration membrane that can be better. There are two sets of mode, and one set is that the suction water source is directly filtered, can take off the filter screen from the device and collect the microorganism, practices thrift the suction filtration time. The other set is that the microorganism is turned down into the incubator by using a handle, and the microorganism is observed by a detecting instrument after being cultured, and finally the microorganism is taken out.
(3) The utility model discloses be equipped with heating rod, LED lamp in the incubator of well adoption, temperature and humidity sensor can realize the real time control to the internal humiture of box, has solved the adverse effect that microorganism culture in-process environment brought, has improved microorganism culture's efficiency.
(4) The utility model discloses in, use the far infrared camera, to the timely record of microbial community, improve work efficiency.
(5) 5 sampling programs are preset, various input interfaces are configured, and the sampling can be completed by connecting with a flowmeter and a liquid level meter.
(6) The precision is high and reliable, the peristaltic pump and the inlet peristaltic tube suction type and mixed stepping motor are driven in a subdivision mode, and the operation is stable and reliable. The method comprises the steps of quantitative sampling at fixed time, quantitative sampling at fixed flow, equal flow proportion at equal time, equal liquid level proportion at equal time, real-time quantitative sampling, automatic sampling at equal time proportion, and setting the sampling time interval of 1-24 hours.
(7) Mechanical distribution arm, automatic positioning, the sampling head is filtered to stainless steel, can realize the fixed point sampling of the different degree of depth, sampling volume: 0-1000 ml/min is adjustable.
(8) The infrared flow detector controls the automatic emptying and cleaning of the pipeline, and fresh and standard water samples are guaranteed to be collected. Has the functions of automatic cleaning and automatic emptying of the sampling pipe (1-2 times of arbitrary setting).
(9) The microorganism tester is used for detecting the number of microorganisms in water, the test solution is filtered by a film during detection, the microorganisms in the test solution are intercepted on the filter film, and then macroscopic colonies are formed by culture and counted to detect the bacteria content in the test solution.
Drawings
FIG. 1 is a schematic structural view of the portable integrated device for sampling, culturing and detecting microorganisms in water body of the present invention;
FIG. 2 is a longitudinal sectional view of the first and second flip-flop filter valves of the integrated device of FIG. 1;
fig. 3 is a top view of fig. 2.
Detailed Description
As can be seen from figure 1, the portable water body microorganism sampling, culturing and detecting integrated device comprises a box body 1, wherein the box body 1 is of a cuboid structure, an ultraviolet lamp 2 is installed at the top in the box body 1, and the box body is sterilized through the ultraviolet lamp when a water sample does not enter a sampling box; the upper part of the box body 1 is provided with a filter box 3, the filter box 3 is connected with an incubator 5 below the filter box through a connecting pipe provided with a first turnbuckle filter valve 4, after a water sample is filtered and transferred to the incubator 5 through the turnbuckle filter valve, a filter membrane can be poured into the incubator 5 through manual turnbuckle, the incubator 5 is connected with a microorganism detector 6 below the incubator 5 through the connecting pipe, the filter box 3 is provided with an infrared flow detector 7 and a water outlet pipe 8, the inlet end of the water outlet pipe 8 is provided with a second turnbuckle filter valve 9, and after water is filtered through the turnbuckle filter valve, the turnbuckle filter valve can be manually turned buckle to pour the filter membrane into the outlet of the water outlet pipe 8 or directly take out the turnbuckle filter screen to take out microorganisms; an oxygen pump 10, a heating rod 11 and a temperature and humidity sensor 12 are mounted on the side wall of the incubator 5, and the heating rod 11 supplies heat for microbial cultivation; the reaction filter plate 13 and the stirring motor 14 are arranged at the bottom of the incubator 5, and the stirring paddle 15 is connected with the motor shaft of the stirring motor 14 through the waveform rotating shaft 16; the microorganism detector 6 is provided with a bacterium taking port 17, a far infrared camera 18 and a micrometer 19, the microorganism detector 6 uses water quality microorganism detection test paper or a kit for detection, the far infrared camera 18 records microorganisms, and the micrometer 19 measures the size of microorganism colonies; the upper end of the box body 1 is provided with a culture liquid groove 20, the culture liquid groove 20 is provided with a liquid adding port 21, and the bottom of the culture liquid groove 20 is communicated with the incubator 5 through a pipe 22; the collecting bottle 23 is arranged in the box body 1, an input interface 24, a storage battery 25, a central processing unit 26, a control panel 27 and a microorganism activity display screen 28 are arranged on one side of the box body 1, a control key 29, a temperature display 30, an oxygen display 31 and a culture solution display 32 are arranged on the control panel 27, the input interface 24 or the storage battery 25 supplies power to the whole device, and the central processing unit 26 controls the temperature and the oxygen in the incubator. The peristaltic pump 33 and the telescopic rod 34 are installed on the other outer side face of the box body 1, one end of the telescopic rod 34 is connected with the peristaltic tube retractor 35, and the telescopic rod 34 facilitates the device to take a water sample at a farther distance; a motor contractor 36 is arranged below the peristaltic tube contractor 35, one end of a peristaltic tube 37 is communicated with the filter box 3, the other end of the peristaltic tube is connected with a funnel-shaped sampling head 38, and two layers of filter screens are arranged in the sampling head 38 to filter silt and float grass in water; the sampling head 38 is connected with a heavy object block 39, and the heavy object block 39 is fixed below the motor retractor 36 through a guide rope 40 with scales, so that the water pipe is in a vertical state under water; and LED lamps 41 are arranged at the tops of the filter box 3 and the incubator 5 to provide light for the device.
The first turning-over filtering valve 4 and the second turning-over filtering valve 9 have the same structure and can be freely taken down from the device as shown in fig. 2 and fig. 3, the structure comprises the first turning-over filtering valve 4 and the second turning-over filtering valve 9, the structure comprises a valve body 13 and a filtering disc 131 in the valve body 13, one side of the filtering disc 131 is fixed with a rotating shaft, the rotating shaft penetrates out of the valve body 13 and is connected with a handle 132, and a filtering film 133 and a filtering net 134 are arranged on the filtering disc 131. The rotary handle 132 drives the filter disc 131 to turn along the rotating shaft, and the filter membrane 133 and the filter screen 134 can fall off.
The using method comprises the following steps:
A. according to different water depths and positions, a guide rope and a motor retractor with scales are used for placing a water intake to a sampling point.
B. The sampling head 38 is extended into the water body, then the peristaltic pump 33 is started, when the peristaltic pump works, the water sample is pumped to the filter box 3 in the box body 1, when the second turning and buckling filter valve 9 works, the second turning and buckling filter valve 4 is closed, the water body can flow out conveniently, after the water is filtered, the filtered microorganism can be turned into the water outlet pipe 8 by the aid of a manual handle, and the microorganism on the filter membrane can be directly taken out by manually taking out the turning and buckling filter valve 9. When the first turning-buckle filter valve 4 works, the second turning-buckle filter valve 9 is closed, so that water can conveniently flow into the filter box, and microorganisms are flushed into the culture box 5 for 2-3 times after the filter membrane is taken down after sampling is completed; and manually operating the handle to turn the filtered microorganisms into the incubator for culture and observation.
C. Then, a valve below the culture liquid tank 20 is opened, and the culture liquid is poured into the incubator 5.
D. A water sample enters the incubator 5, if necessary, the heating rod 11 on the wall of the openable incubator is heated to heat the culture solution, so that water microorganisms react with the culture solution, and meanwhile, the temperature and humidity sensor 12 collects the temperature and humidity in the incubator 5 in real time.
E. The liquid after cultivation is detected by a valve under the incubator 5 to a microorganism detector 6.
F. The microorganism sample enters the detection chip through the channel, the detection chip rapidly detects molecules contained in the water microorganism, and the micrometer 19 is used for observing the size of the microorganism.
A working power supply: a direct current 12V lithium battery, or 220V alternating current charging, or automobile power supply charging.
The water quality microbe detection test paper sheet or kit can detect more than ten microbe and bacteria indexes in water quality, has loose requirements on detection environment, can be used in a laboratory, and can also be used for mobile detection. The integrated device is light in weight and convenient to carry, has the functions of manual sampling and automatic sampling, and is convenient for workers to use, and the LED display window and the Chinese menu display are realized. And (3) alarm mode: sound, indicator light. Based on the utility model discloses an implementation case, all other implementation cases that ordinary person in the art obtained under the prerequisite of not making creative work all belong to the utility model's scope of protection.
Claims (6)
1. The utility model provides a portable water microorganism sample is cultivateed and is detected integrated device which characterized by: the device comprises a box body (1), an ultraviolet lamp (2) is installed at the top in the box body (1), a filter box (3) is arranged on the upper portion of the box body (1), the filter box (3) is connected with an incubator (5) below the filter box through a connecting pipe provided with a first turning and buckling filter valve (4), the incubator (5) is connected with a microorganism detection meter (6) below the incubator (5) through a connecting pipe, an infrared flow detector (7) and a water outlet pipe (8) are arranged on the filter box (3), a second turning and buckling filter valve (9) is arranged at the inlet end of the water outlet pipe (8), an oxygen pump (10), a heating rod (11) and a temperature and humidity sensor (12) are installed on the side wall of the incubator (5), a stirrer is installed at the bottom of the incubator (5), and a bacterium taking port (17), a far infrared camera (18) and a micrometer (19) are arranged on the microorganism detection meter (6); the upper end of the box body (1) is provided with a culture liquid tank (20), the culture liquid tank (20) is provided with a liquid feeding port (21), and the bottom of the culture liquid tank (20) is communicated with the incubator (5) through a pipe (22); an input interface (24), a storage battery (25), a central processing unit (26), a control panel (27) and a microorganism movable display screen (28) are arranged on one side of the box body (1), a peristaltic pump (33) and a telescopic rod (34) are arranged on the other outer side surface of the box body (1), and one end of the telescopic rod (34) is connected with a peristaltic tube retractor (35); motor contractor (36) are installed to the below of peristaltic tube contractor (35), the one end of peristaltic tube (37) with rose box (3) intercommunication, the other end be connected with hourglass hopper-shaped sampling head (38), sampling head (38) link to each other with heavy object piece (39), heavy object piece (39) are fixed in motor contractor (36) below through messenger (40) that are equipped with the scale.
2. The portable integrated device for sampling, culturing and detecting microorganisms in water body as claimed in claim 1, wherein: the stirrer comprises a stirring motor (14) arranged at the bottom of the incubator (5), and a stirring paddle (15) is connected with a motor shaft of the stirring motor (14) through a waveform rotating shaft (16).
3. The portable integrated device for sampling, culturing and detecting microorganisms in water body as claimed in claim 1, wherein: a collecting bottle (23) is arranged in the box body (1).
4. The portable integrated device for sampling, culturing and detecting microorganisms in water body as claimed in claim 1, wherein: the control panel (27) is provided with a control key (29), a temperature display (30), an oxygen display (31) and a culture solution display (32).
5. The portable integrated device for sampling, culturing and detecting microorganisms in water body as claimed in claim 1, wherein: and an LED lamp (41) is arranged at the top of the incubator (5).
6. The portable integrated device for sampling, culturing and detecting microorganisms in water body as claimed in claim 1, wherein: first turn over detain strainer valve (4) and second turn over the structure of detaining strainer valve (9) the same, filter disc (131) in valve body (13) and valve body (13), one side and the pivot of filter disc (131) are fixed, valve body (13) are worn out in the pivot and handle (132) are connected, place filtration membrane (133) and filter screen (134) on filter disc (131).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221989691.9U CN218321393U (en) | 2022-07-30 | 2022-07-30 | Portable water microorganism sampling culture and detection integrated device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221989691.9U CN218321393U (en) | 2022-07-30 | 2022-07-30 | Portable water microorganism sampling culture and detection integrated device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN218321393U true CN218321393U (en) | 2023-01-17 |
Family
ID=84872863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221989691.9U Withdrawn - After Issue CN218321393U (en) | 2022-07-30 | 2022-07-30 | Portable water microorganism sampling culture and detection integrated device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN218321393U (en) |
-
2022
- 2022-07-30 CN CN202221989691.9U patent/CN218321393U/en not_active Withdrawn - After Issue
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240312 Address after: Building 1, 2003, Xincheng District (Yongfeng Chamber of Commerce Building), Enjiang Town, Yongfeng County, Ji'an City, Jiangxi Province, 331599 Patentee after: Jiangxi Haiquan Yongfa Water Conservancy Construction Engineering Co.,Ltd. Country or region after: China Address before: 246133 No. 1318 Jixian North Road, Anqing City, Anhui Province Patentee before: ANQING NORMAL University Country or region before: China |
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| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20230117 Effective date of abandoning: 20240401 |
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| AV01 | Patent right actively abandoned |
Granted publication date: 20230117 Effective date of abandoning: 20240401 |