CN218282887U

CN218282887U - Western blot membrane washing device

Info

Publication number: CN218282887U
Application number: CN202222927099.2U
Authority: CN
Inventors: 靳浩; 张亚豪; 胡薇薇; 陆文彬; 丁建东
Original assignee: Zhongda Hospital of Southeast University
Current assignee: Zhongda Hospital of Southeast University
Priority date: 2022-11-03
Filing date: 2022-11-03
Publication date: 2023-01-13
Anticipated expiration: 2032-11-03

Abstract

The utility model discloses a membrane device is washed to western blot, include: a first water tank, a siphon water tank and a membrane washing box; the first water tank is of a closed structure, a vertical partition plate is arranged in the first water tank, the bottom of the partition plate is fixed with the inner wall of the first water tank, and a certain distance is reserved between the top of the partition plate and the inner wall of the first water tank, so that the water tank is divided into a waste water tank and a water replenishing tank, wherein the tops of the waste water tank and the water replenishing tank are communicated through the partition plate; the siphon water tank is communicated with the water supplementing tank through a first water pipe, and a fluid pump is further arranged on the first water pipe and connected with the timer; the membrane washing box is communicated with the siphon water tank through a siphon pipe, the inlet end of the siphon pipe is positioned in the siphon water tank, the outlet end of the siphon pipe is positioned in the membrane washing box, the inlet end of the siphon pipe is higher than the water outlet end, and the membrane washing box is connected with the wastewater tank through a second water pipe; the utility model provides an automatic membrane device of washing of western blot.

Description

Western blot membrane washing device

Technical Field

The utility model belongs to the technical field of Western blot experimental apparatus, concretely relates to Western blot membrane washing device.

Background

Western blotting (immunoblotting assay) is known as WesternBlot. It is a commonly used experimental method in molecular biology, biochemistry and immunogenetics, proposed in 1979 by HarryTowbin, the Institute for the biology of Friedrich Miescher Institute (Friedrich Miescher Institute), switzerland, first known as westerblot in "Analytical Biochemistry" (Analytical Biochemistry) authored in 1981 by Neal Burnette (Neal Burnette), the westerblot experiment, whose steps roughly include sample preparation, protein content quantification, electrophoresis, membrane transfer (PVDF membrane or NC membrane), blocking, membrane washing, antibody incubation, chemiluminescence, etc., and the whole process is time-consuming.

It washes membrane operation to relate to many times after changeing membrane, the closed operation, usually for TBST liquid washing membrane (strip) 4 ~ 5 times, 5 minutes at every turn, needs frequent manual change TBST liquid in the experimentation, complex operation causes easily when manual change to wash membrane flow unstable, causes washing liquid to supply untimely, the problem of dry film appears, based on this, it is necessary to research and develop a western blot automatic membrane device.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a membrane device is washed to western blot has solved the current problem of washing the membrane device and need frequent manual change TBST liquid in the experimentation.

The utility model discloses a technical scheme be, a western blot washes membrane device, include:

the first water tank is of a closed structure, a vertical partition plate is arranged in the first water tank, and the partition plate divides the water tank into a waste water tank and a water supplementing tank, wherein the tops of the waste water tank and the water supplementing tank are communicated;

the siphon water tank is communicated with the water supplementing groove through a first water pipe, and a fluid pump is further arranged on the first water pipe;

the membrane washing box is communicated with the siphon water tank through a siphon pipe, the inlet end of the siphon pipe is positioned in the siphon water tank, the outlet end of the siphon pipe is positioned in the membrane washing box, meanwhile, the inlet end of the siphon pipe is higher than the water outlet end, and the membrane washing box is connected with the wastewater tank through a second water pipe.

The fluid pump is connected with the timer.

The first water tank, the siphon water tank, the first water pipe, the fluid pump, the siphon pipe and the second water pipe are all arranged in a protective box body.

An open groove for introducing a siphon is further formed in the membrane washing box, and a membrane inlet is formed in the side wall, located on the outer side of the protection box body, of the membrane washing box.

A water replenishing port is arranged on the outer side of the water replenishing tank.

The side wall of the waste water tank is provided with a water outlet for draining water.

Sealing plugs are arranged at the water replenishing port and the water draining port.

The fluid pump is a peristaltic pump.

The utility model has the advantages that:

the utility model discloses a western blot membrane washing device, which depends on the combination of a peristaltic pump and a siphon device, has stable liquid flow, can supplement new washing liquid in time, and can avoid the mixing of the new and old washing liquids;

the utility model discloses a western blot membrane washing device can add various liquids such as TBST, antibody, water to the moisturizing groove, and the on-off time of adjustment timer accomplishes different experimental purposes, has also guaranteed the repeatability of experiment simultaneously.

Drawings

FIG. 1 is a schematic structural view of a western blot membrane washing device of the present invention;

figure 2 is a perspective view of the western blot membrane washing device of the utility model.

In the figure, 1, a fluid pump, 2, a siphon water tank, 3, a membrane washing box, 4, a second water pipe, 5, a waste water tank, 6, a partition plate, 7, a water supplementing tank, 8, a first water pipe, 9, a siphon pipe, 10, a first water tank and 11, a protective box body are arranged.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings and specific embodiments. The following examples are only used to illustrate the technical solutions of the present invention more clearly, and the protection solutions of the present invention are not limited thereto.

Example 1

The utility model discloses a membrane device is washed to western blot, include: a first water tank 10, a siphon water tank 2 and a membrane washing box 3; the first water tank 10 is of a closed structure, a vertical partition plate 6 is arranged in the first water tank 10, the bottom of the partition plate 6 is fixed with the inner wall of the first water tank 10, and a certain distance is reserved between the top of the partition plate 6 and the inner wall of the first water tank, so that the water tank is divided into a waste water tank 5 and a water replenishing tank 7, the tops of which are communicated by the partition plate 6; the siphon water tank 2 is communicated with the water supplementing tank 7 through a first water pipe 8, and a fluid pump 1 is further arranged on the first water pipe 8; the membrane washing box 3 is communicated with the siphon water tank 2 through a siphon 9, the inlet end of the siphon 9 is positioned in the siphon water tank 2, the outlet end of the siphon 9 is positioned in the membrane washing box 3, meanwhile, the inlet end of the siphon 9 is higher than the water outlet end, and the membrane washing box 3 is connected with the waste water tank 5 through a second water pipe 4.

Furthermore, a water replenishing port is arranged on the outer side of the water replenishing groove 7, and a water discharging port for discharging water is arranged on the side wall of the wastewater groove 5. Sealing plugs are arranged at the water replenishing port and the water draining port. Before working, washing liquid required to be used is injected into the water supplementing groove through the water supplementing opening, and during working, the waste water groove 5 and the water supplementing groove 7 are both sealed by the sealing plug.

The first tank 10, the siphon tank 2, the first water pipe 8, the fluid pump 1, the siphon pipe 9, and the second water pipe 4 are disposed inside one protective case 11. Thus, the utility model can be integrally arranged on the shaking bed, thereby not only omitting the shaking bed device, but also avoiding the contact of toxic substances.

In order to normally operate the device, an open groove for introducing the siphon 9 is further formed in the membrane washing box 3, and a membrane inlet is formed in the side wall, located on the outer side of the protective box body 11, of the membrane washing box 3, so that the membrane can be conveniently taken out.

The fluid pump 1 is a peristaltic pump and is connected with a timer, the peristaltic pump is controlled by the timer, the timer is started for 5 seconds, when the timer is opened, liquid can be injected into the siphon water tank 2 through the first water pipe, meanwhile, the liquid washed in the siphon water tank 3 can be pumped out depending on negative pressure formed after the peristaltic pump is started, after the liquid in the siphon water tank 3 is pumped out, the liquid in the siphon water tank 2 is just injected into a siphon water level, then, depending on siphon action, the washing liquid is injected into the siphon water tank 3 and then begins a new round of washing, the process depends on the timer, the peristaltic pump is controlled by the timer to be closed for 10 minutes, the time for washing the membrane is 5 seconds, the time for closing is 10 minutes, and after the operation is finished, the washed liquid can finally enter the waste water tank 5 and finally can flow out through the water outlet.

The above-mentioned embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereto, and any person skilled in the art can obviously obtain simple changes or equivalent replacements of the technical solutions within the technical scope of the present invention.

Claims

1. The utility model provides a western blot membrane washing device which characterized in that includes:

the first water tank (10) is of a closed structure, a vertical partition plate (6) is arranged in the first water tank (10), and the partition plate (6) divides the water tank into a waste water tank (5) and a water supplementing tank (7) with communicated tops;

the siphon water tank (2) is communicated with the water supplementing tank (7) through a first water pipe (8), and a fluid pump (1) is further arranged on the first water pipe (8);

wash diaphragm chamber (3), with communicate through siphon (9) between siphon water tank (2), the entry end of siphon (9) is located siphon water tank (2), and the exit end of siphon (9) is located wash diaphragm chamber (3), simultaneously the entry end of siphon (9) is higher than the outlet end, wash diaphragm chamber (3) with connect through second water pipe (4) between wastewater disposal basin (5).

2. A western blot membrane washing apparatus according to claim 1, wherein the fluid pump (1) is connected to a timer.

3. A western blot membrane washing device according to claim 1, wherein the first water tank (10), the siphon water tank (2), the first water pipe (8), the fluid pump (1), the siphon tube (9) and the second water pipe (4) are all arranged inside a protective tank (11).

4. A western blot membrane washing device according to claim 3, wherein the membrane washing box (3) is further provided with an open slot for introducing the siphon (9), and the side wall of the membrane washing box (3) on the outer side of the protective box body (11) is provided with a membrane inlet.

5. A western blot membrane washing device according to claim 1, wherein a water replenishing port is provided on the outer side of the water replenishing tank (7).

6. A western blot membrane washing device according to claim 5, wherein a drain port for draining water is provided on the side wall of the wastewater tank (5).

7. The western blot membrane washing device according to claim 6, wherein the water supply port and the water discharge port are both provided with sealing plugs.

8. A western blot membrane washing apparatus according to claim 1, wherein said fluid pump is a peristaltic pump.