CN218271957U

CN218271957U - Visual nucleic acid detection device

Info

Publication number: CN218271957U
Application number: CN202222582387.9U
Authority: CN
Inventors: 陈帅印; 沈悦; 段广才; 袁明珠; 龙金照; 晋乐飞; 杨海燕
Original assignee: Zhengzhou University
Current assignee: Zhengzhou University
Priority date: 2022-09-27
Filing date: 2022-09-27
Publication date: 2023-01-10
Anticipated expiration: 2032-09-27

Abstract

The utility model discloses a visual nucleic acid detection device, which comprises a detection box, wherein one side of the upper end of the detection box is provided with an observation port, a heating block is arranged below the observation port, and the heating block is arranged at the bottom of the detection box; the middle part of the detection box is provided with a first clapboard, the first clapboard divides the detection box close to one side of the observation port into detection cavities, and the first clapboard is obliquely arranged towards the observation port; the observation port is packaged through the transparent acrylic plate, a drawable structure is arranged between the observation port and the acrylic plate, a second partition plate is arranged in the detection cavity opposite to the first partition plate, the second partition plate is vertically arranged, and LED lamps for emitting light sources with different wavelengths are arranged on the first partition plate and the second partition plate respectively. This scheme uses the heating block to heat for whole observation chamber, and is with low costs, and very portable, and LED lamp and inferior gram force board are not fixed completely in equipment, and the user can change according to self experiment needs.

Description

Visual nucleic acid detection device

Technical Field

The utility model relates to a detection device field, concretely relates to visual nucleic acid detection device.

Background

With the continuous development and innovation of CRISPR-Cas system, CRISPR diagnosis has recently become a powerful rapid diagnostic tool. The instant detection technology based on the CRISPR platform of the invention enables a diagnostician to quickly receive and easily interpret the results, which is a continuously pursued goal in the field of molecular diagnosis.

At present, portable fluorescence visualization equipment mainly comprises a blue light gel cutting instrument and self-made handheld equipment. A general blue light gel cutting instrument consists of a blue light LED lamp, a glass table top and an amber light filter, has the characteristics of small volume, portability and the like, but has the advantages that when the blue light gel cutting instrument is used for visualizing a CRISPR detection result, the light source is unique, the typing detection cannot be realized, and the application in the field of CRISPR detection is limited. Besides, researchers have made a handheld visualization device (https:// doi.org/10.1016/j.bios.2021.113701) which is mainly composed of two light sources emitting different wavelengths, and different excitation filters and emission filters, and can simultaneously detect and type HPV16 and HPV18, showing fluorescence of different colors. However, the narrow-band filters used in this device are expensive (about 500 yuan/piece), and the cost of constructing a handheld visualization device with only filters is about 2000 yuan, which limits the spread and use of the instrument in economically undeveloped areas.

SUMMERY OF THE UTILITY MODEL

The above-mentioned not enough to prior art, the utility model provides a use convenient, with low costs visual nucleic acid detecting device.

In order to achieve the purpose of the invention, the technical scheme adopted by the utility model is as follows:

the visual nucleic acid detection device comprises a detection box, wherein an observation port is formed in one side of the upper end of the detection box, a heating block is arranged below the observation port, and the heating block is placed at the bottom of the detection box; the middle part of the detection box is provided with a first clapboard, the first clapboard divides the detection box close to one side of the observation port into detection cavities, and the first clapboard is obliquely arranged towards the observation port; the observation port is packaged through a transparent acrylic plate, a drawable structure is arranged between the observation port and the acrylic plate, a second partition plate is arranged in the detection cavity opposite to the first partition plate, the second partition plate is vertically arranged, LED lamps emitting light sources with different wavelengths are respectively arranged on the first partition plate and the second partition plate, and the LED lamps are electrically connected with a controller in the detection box.

Furthermore, but pull structure is for setting up the C shape spout at detection chamber upper end both sides border, and the ya keli board inserts in the both ends C shape spout, and the border one end opening that the C shape spout is close to the detection case.

Furthermore, a first LED lamp panel and a second LED lamp panel are arranged on the first partition plate and the second partition plate respectively, LED lamps of different wavelength light sources are arranged on the first LED lamp panel and the second LED lamp panel respectively, supporting columns are arranged on four corners of the bottoms of the first LED lamp panel and the second LED lamp panel respectively, and adsorption devices adsorbed on the first partition plate or the second partition plate are arranged at the end portions of the supporting columns.

Furthermore, the adsorption device is a magnet, and the first partition plate and the second partition plate are both iron plates.

Furthermore, a storage battery and a controller are arranged in one side, away from the detection cavity, of the detection box, a power jack and a switch button are arranged on the side face of the outside of the detection box, and the lamp panel, the storage battery, the power jack and the switch button are all electrically connected with the controller.

Furthermore, the LED lamp on the first partition board is a lamp bead with the wavelength of 530nm, and the LED lamp on the second partition board is a lamp bead with the wavelength of 470 nm.

The utility model has the advantages that: the heating block is used for heating the whole observation cavity, so that the device is low in cost and very portable, and can replace a metal bath to provide a proper incubation temperature for detection and amplification of a target before detection; the same detection and typing can be achieved by replacing a narrow-band filter with a cheap acrylic plate, but the cost can be greatly reduced; the utility model discloses an equipment is small, can be used to the witnessed inspections, not only can accomplish the signal reading out of 96 samples at most simultaneously, can also realize the typing and detect. Neither the LED lamp nor the acrylic plate are completely fixed in the equipment, and a user can replace the LED lamp and the acrylic plate according to the experimental needs of the user.

The LED lamps with different wavelengths can excite the cut fluorescent groups to emit light with different wavelengths, and the acrylic plates with different colors can filter and absorb light with different colors. The LED lamps with the wavelengths of 470nm and 530nm are used for exciting green fluorescence of FAM groups and red fluorescence of ROX groups respectively, blue is filtered through an amber acrylic plate to penetrate through the green, the green fluorescence emitted by the FAM groups can be observed, the red fluorescence emitted by the ROX groups is excited through the 530nm LED lamps, the green is filtered through the red acrylic plate to penetrate through the red, and the red fluorescence emitted by the ROX groups can be observed. And finally, judging whether the result is negative or positive according to the existence of fluorescence under different combinations of the LED lamp and the acrylic plate, thereby achieving the purposes of detection and typing.

Drawings

FIG. 1 is a schematic diagram showing a nucleic acid detecting apparatus.

FIG. 2 is a view showing the internal structure of a nucleic acid detecting apparatus.

The LED lamp comprises a detection box 1, a detection box 2, a switch button 3, a power supply jack, a C-shaped sliding groove 4, an acrylic plate 5, a heating block 6, a first LED lamp plate 7, a first partition plate 8, a second LED lamp plate 9, a second partition plate 10, a support column 11, a magnet 12, a storage battery 13 and a second partition plate.

Detailed Description

The following description of the embodiments of the present invention is provided to facilitate the understanding of the present invention by those skilled in the art, but it should be understood that the present invention is not limited to the scope of the embodiments, and various changes may be made apparent to those skilled in the art within the spirit and scope of the present invention as defined and defined by the appended claims.

As shown in fig. 1 and 2, the visual nucleic acid detecting device of the scheme comprises a detecting box 1, an observation port is formed in one side of the upper end of the detecting box 1, a heating block 6 is arranged below the observation port, the heating block 6 can be an existing warmer, the cost is low, the replacement is convenient, and the heating block 6 is placed at the bottom of the detecting box 1; a first partition plate 8 is arranged in the middle of the detection box 1, the detection box 1 close to one side of the observation port is divided into detection cavities by the first partition plate 8, and the first partition plate 8 is obliquely arranged towards the observation port; the observation port is packaged through the transparent acrylic plate 5, a drawable structure is arranged between the observation port and the acrylic plate 5, a second partition plate 13 is arranged in the detection cavity opposite to the first partition plate 8, the second partition plate 13 is vertically arranged, LED lamps which emit light sources with different wavelengths are respectively arranged on the first partition plate 8 and the second partition plate 13, and the LED lamps are electrically connected with a controller in the detection box.

The heating block 6 is used for heating the whole observation cavity, so that the device is low in cost and very portable, and can replace a metal bath to provide a proper incubation temperature for detection and amplification of a target before detection; the same detection and parting effects can be achieved by replacing a narrow-band filter with the cheap acrylic plate 5, but the cost can be greatly reduced; the utility model discloses an equipment is small, can be used to the witnessed inspections, not only can accomplish the signal reading out of 96 samples at most simultaneously, can also realize the typing and detect. Neither the LED lamp nor the acrylic plate 5 are completely fixed in the device, and the user can replace the LED lamp and the acrylic plate according to the experimental needs of the user.

In this embodiment, but the pull structure is for setting up in the C shape spout 4 that detects chamber upper end both sides border, and inferior gram force board 5 inserts in both ends C shape spout 4, and C shape spout 4 is close to the border one end opening of detection case 1. When the acrylic plates 5 with different colors are replaced, the drawing operation is only needed, and the operation is convenient.

In this embodiment, a first LED lamp panel 7 and a second LED lamp panel 9 are respectively disposed on a first partition plate 8 and a second partition plate 13, LED lamps of different wavelength light sources are respectively disposed on the first LED lamp panel 7 and the second LED lamp panel 9, support columns 10 are disposed at four corners of bottoms of the first LED lamp panel 7 and the second LED lamp panel 9, and an adsorption device adsorbed on the first partition plate 8 or the second partition plate 13 is disposed at an end of each support column 10; adsorption equipment is magnet 11, and first baffle 8 and second baffle 13 are iron plate, conveniently change first LED lamp plate 7 and second LED lamp plate 9, and first LED lamp plate 7 and second LED lamp plate 9 are connected with the controller electricity through the cable plug that sets up on it, and the controller adopts the PCB board that carries on the C51 singlechip.

In this embodiment, be provided with battery 12 and controller in keeping away from one side of detecting the chamber in the detection case 1, the outside side of detection case 1 is provided with electrical socket 3 and shift knob 2, and lamp plate, battery 12, electrical socket 3 and shift knob 2 all are connected with the controller electricity, and battery 12 adopts rechargeable battery, makes things convenient for recycling.

In this embodiment, the LED lamp on the first partition plate 8 is a lamp bead with a wavelength of 530nm, and the LED lamp on the second partition plate 13 is a lamp bead with a wavelength of 470 nm.

The LED lamps with different wavelengths can excite the cut fluorescent groups to emit light with different wavelengths, and the acrylic plates 5 with different colors can filter and absorb the light with different wavelengths. The device uses 470nm and 530nm wavelength LED lamps which are respectively used for exciting green fluorescence of FAM groups and red fluorescence of ROX groups, blue color is filtered through an amber acrylic plate 5 to penetrate through green color, the green fluorescence emitted by the FAM groups can be observed, the 530nm LED lamps excite the red fluorescence of the ROX groups, the green color is filtered through the red acrylic plate 5 to penetrate through red color, and the red fluorescence emitted by the ROX groups can be observed. And finally, judging whether the result is negative or positive according to the existence of fluorescence under different combinations of the LED lamps and the acrylic plates 5, thereby achieving the purposes of detection and typing.

The method for testing by using the equipment of the embodiment comprises the following steps: (for better illustration of the results, this example discusses the HPV16/18 rapid detection experiment based on the CRISPR-Cas13a/Cas12a dual-channel system, in which the Cas12a detection system is used for detecting HPV18 and the used fluorophore is ROX; the Cas13a system is used for detecting HPV16 and the used fluorophore is FAM).

Step 1: firstly, a disposable heater (warmer) is placed, then the sample tube added with the detection system is placed on the disposable heater for incubation, and fluorescence can be observed after the detection system is incubated for 10-30 minutes.

Step 2: an amber acrylic plate 5 is installed through a C-shaped sliding groove 4, then a 470nm LED lamp is turned on through a switch button 2, whether the sample tube emits green fluorescence or not is observed through the acrylic plate 5 by naked eyes, the sample tube is shot by a mobile phone, and then the 470nm LED lamp is turned off. The fluorescent material was positive if there was green fluorescence, and was negative if there was no green fluorescence.

And step 3: similar to the step 2, firstly, a red acrylic plate 5 is installed through a C-shaped sliding groove 4, then the 530nm LED lamp is turned on through a switch button 2, whether the sample tube emits red fluorescence or not is observed through the acrylic plate 5 by naked eyes and shot by a mobile phone, and then the 530nm LED lamp is turned off. The fluorescence was positive in the presence of red fluorescence, and negative in the absence of red fluorescence.

And 4, step 4: judging the result, if green fluorescence is observed in the step 2 and red fluorescence is observed in the step 3, confirming that the detected sample has two genotypes, namely the sample is positive to HPV 16/18; if green fluorescence is observed in step 2 and no red fluorescence is observed in step 3, the detected sample is proved to be positive HPV16 and negative HPV 18; if no green fluorescence is observed in the step 2 and red fluorescence is observed in the step 3, the detected sample is proved to be HPV16 negative and HPV18 positive; if no green fluorescence or red fluorescence is observed in step 2 and step 3, the test sample is confirmed to be HPV16/18 negative.

Claims

1. A visual nucleic acid detection device is characterized by comprising a detection box, wherein one side of the upper end of the detection box is provided with an observation port, a heating block is arranged below the observation port, and the heating block is placed at the bottom of the detection box; the middle part of the detection box is provided with a first clapboard, the first clapboard divides the detection box close to one side of the observation port into detection cavities, and the first clapboard is obliquely arranged towards the observation port; the utility model discloses a light source detection box, including observation mouth, first baffle, second baffle, LED lamp, controller electricity, survey the mouth and pass through transparent ya keli board encapsulation, but be provided with the pull structure between observation mouth and the ya keli board, it is provided with the second baffle to detect the intracavity relative with first baffle, the vertical setting of second baffle, be provided with the LED lamp that sends different wavelength light sources on first baffle and the second baffle respectively, the LED lamp is connected with the controller electricity in the detection case.

2. The visual nucleic acid detecting device according to claim 1, wherein the drawable structure is a C-shaped sliding groove disposed at the edge of the upper end of the detecting cavity, the acrylic plate is inserted into the C-shaped sliding groove at both ends, and the C-shaped sliding groove is opened at one end near the edge of the detecting box.

3. The visual nucleic acid detection device according to claim 1, wherein the first partition plate and the second partition plate are respectively provided with a first LED lamp panel and a second LED lamp panel, the LED lamps of different wavelength light sources are respectively arranged on the first LED lamp panel and the second LED lamp panel, the four corners of the bottoms of the first LED lamp panel and the second LED lamp panel are respectively provided with a support column, and the end parts of the support columns are provided with adsorption devices adsorbed on the first partition plate or the second partition plate.

4. The visual nucleic acid detecting device according to claim 3, wherein the adsorbing means is a magnet, and the first and second partition plates are both iron plates.

5. The visual nucleic acid detection device according to claim 3, wherein a storage battery and a controller are arranged in one side of the detection box far away from the detection cavity, a power jack and a switch button are arranged on the side surface of the outside of the detection box, and the lamp panel, the storage battery, the power jack and the switch button are all electrically connected with the controller.

6. The visual nucleic acid detection device of claim 1, wherein the LED lamp on the first partition board is a lamp bead with a wavelength of 530nm, and the LED lamp on the second partition board is a lamp bead with a wavelength of 470 nm.