CN218239728U - Aquatic microorganism on-line measuring device - Google Patents
Aquatic microorganism on-line measuring device Download PDFInfo
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- CN218239728U CN218239728U CN202221862076.1U CN202221862076U CN218239728U CN 218239728 U CN218239728 U CN 218239728U CN 202221862076 U CN202221862076 U CN 202221862076U CN 218239728 U CN218239728 U CN 218239728U
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Abstract
The utility model discloses an aquatic microorganism on-line measuring device, set up laser emission system and produce the focus, the compressed rectangle facula shines the sample particle that awaits measuring that flows through flow chamber liquid way pipeline, make the sample particle that awaits measuring produce and take place the scattering or/and produce intrinsic fluorescence, receive by scattered light receiving system respectively and turn into current signal, fluorescence receiving system absorbs and turns into current signal, amplify into voltage signal through corresponding preamplifier current signal again, high frequency noise in the signal is filtered through the wave filter by the signal processing system, convert into digital signal through the AD converter again and convey to the host computer, carry out analysis, processing such as demonstration, calculate the particle size of each particle, finally obtain the quantity of the particle in each particle diameter section; or the fluorescence intensity information emitted by the microbial particles can be obtained, and the microbial particles can be simply classified and counted. And meanwhile, the identification and concentration calculation of the microbial particles and the non-microbial particles are realized.
Description
Technical Field
The utility model relates to an aquatic microorganism and particulate matter detection field, concretely relates to but online microorganism detection device of concentration, quantity, particle diameter of real-time supervision aquatic microorganism particulate matters such as bacterium, fungi.
Background
The microbial particles in water mainly contain various organic molecules, wherein the main fluorescent substances are tryptophan, nicotinamide Adenine Dinucleotide (NADH), riboflavin and the like. These metabolites of the organism emit intrinsic fluorescence under the irradiation of ultraviolet light or blue-violet light, wherein, the fluorescence emission spectrum range of Nicotinamide Adenine Dinucleotide (NADH) is mainly between 480 and 600nm, and 490 to 530nm is the strongest signal; the spectrum of the fluorescence emission of riboflavin is mainly between 500 and 620nm, with 530 to 560nm being the strongest signal. Different microbial particles often contain different components, such as bacteria, fungi and spores, which often contain various organic molecules, and viruses and toxins which generally only contain amino acid components. Therefore, different microbial particles exhibit different light absorption and emission characteristics.
The monitoring of the microorganisms in the water is suitable for evaluating other environments with high requirements on water sample cleanliness, such as drinking water for life, medical water of pharmaceutical enterprises, water for injection and the like.
The existing microorganism detection method mainly comprises an agar plate culture method: a water sample is taken from a water source and placed on a culture medium of a culture dish, visible colonies are propagated under appropriate conditions for counting, the number of microorganisms in a clean environment is judged according to the number of the colonies in the culture dish, and the cleanliness of microbial particles in a target water source is evaluated according to the number of the microorganisms.
The above prior art methods have the following disadvantages:
(1) the sample to be detected is not easy to be dispersed into single cells completely, so the formed colonies are not all single colonies, and a part of the formed colonies are grown by more than two cells, so the detection result of the plate colony counting is lower than the actual colony number.
(2) Some bacteria cannot be detected by culture, or the culture medium can only select partial strains to be cultured, thereby limiting the application range of the method.
(3) The method can not realize on-line real-time monitoring, resulting in long detection period.
Disclosure of Invention
The utility model provides a real-time online detection device for microorganisms in water, which solves the problems existing in the prior art in use.
The utility model provides a current technical scheme who has the problem be: an in-water microorganism on-line detection device comprises
The laser emission system comprises a semiconductor laser, a collimating lens, a first flat convex cylindrical lens which is coaxial with the collimating lens, and a second flat convex cylindrical lens which is coaxial with the collimating lens, wherein the first and second convex cylindrical lenses are vertically matched with each other, focused and compressed light spots are rectangular light spots, convex surfaces of the first and second convex cylindrical lenses are used as light incidence surfaces, and a plane is used as a light emission surface;
the flow chamber is provided with a liquid pipeline, and the liquid pipeline is arranged on the optical axis and is positioned at a rectangular light spot formed by matching, focusing and compressing the first and second convex cylindrical lenses;
the device comprises a laser emission system, a scattered light receiving system and a scattered light receiving system, wherein the scattered light receiving system is arranged on the opposite side of the laser emission system and used for receiving scattered light generated by sample particles to be detected in a liquid path pipeline and comprises a diaphragm, a first aspheric lens, a long-wave cut-off filter, a first plano-convex lens and a scattered light detector which are sequentially arranged along the same optical axis, the convex surface of the first plano-convex lens is a light incidence surface, the plane of the first plano-convex lens is a light emission surface, and the scattered light detector is connected with a scattered light preamplifier;
the fluorescence receiving system is arranged on one side of the laser emission system and used for receiving fluorescence generated by sample particles to be detected in the liquid pipeline, the optical axis of the fluorescence receiving system is perpendicular to that of the laser emission system, the fluorescence receiving system comprises a second aspheric lens, a short-wave cut-off filter, a second plano-convex lens and a fluorescence detector, the second aspheric lens, the short-wave cut-off filter, the second plano-convex lens and the fluorescence detector are sequentially arranged along the same optical axis, the convex surface of the second plano-convex lens is a light incidence surface, the plane of the second plano-convex lens is a light emission surface, and the fluorescence detector is connected with a fluorescence preamplifier;
the signal processing system comprises a filter, an A/D converter and an upper computer which are connected in sequence, wherein the filter is connected with a scattered light preamplifier and a fluorescence preamplifier.
As a further improvement, the central axis of the liquid pipeline is intersected with the optical axis of the laser emission system.
As a further improvement, the semiconductor laser emits 405nm laser.
As a further improvement, the diaphragm is arranged at the center of the light incidence surface of the first aspheric lens.
As a further improvement, the minimum detection resolution of the liquid pipeline is one microorganism particle; the scattered light detector is a photodiode; the fluorescence detector is an avalanche photodiode or a photomultiplier.
Compared with the prior art, set up laser emission system and produce the focus, the compressed rectangle facula shines the sample particle that awaits measuring that flows through flow room liquid path pipeline, make the sample particle that awaits measuring produce and take place the scattering or/and produce intrinsic fluorescence, thereby receive by scattered light receiving system respectively and turn into current signal, fluorescence receiving system absorbs and turns into current signal, through corresponding preamplifier current signal amplification for voltage signal again, high frequency noise in the final signal of being filtered through the wave filter by signal processing system, convert into digital signal through the AD converter again and convey to the host computer, carry out analysis, processing such as demonstration, calculate the particle size of each particle, finally obtain the quantity of the particle in each particle diameter section; or the fluorescence intensity information emitted by the microorganism particles can be obtained, so that the microorganism particles can be simply classified and counted. The device has the advantages that the identification and concentration calculation of the microbial particles and the non-microbial particles can be realized simultaneously, and the internal structure of the device has the advantages of low equipment manufacturing difficulty, high accuracy and the like.
The utility model discloses a device can monitor the concentration of aquatic microorganism particulate matter and non-microorganism particulate matter simultaneously, distinguishes through the discernment to microorganism particulate matter and non-microorganism particulate matter, has realized concentration, the quantity of microorganism particulate matter in the real-time supervision air and has improved the monitoring rate of accuracy.
Drawings
Fig. 1 is a schematic structural diagram of a laser emission system of the present invention.
Fig. 2 is a structural schematic diagram of a test of matching of a liquid pipeline and a detection focus light spot.
Fig. 3 is a schematic view of the structure principle of the detecting device of the present invention.
Fig. 4 is a schematic structural diagram of the signal processing system of the present invention.
Detailed Description
Referring to fig. 1-4, the present embodiment is an online detection apparatus for microorganisms in water, including a laser emission system, a flow chamber, a scattered light receiving system, a fluorescence receiving system, and a signal processing system.
The laser emission system comprises a semiconductor laser 101, a collimating lens 102, a first plane-convex cylindrical lens which is coaxial with the collimating lens 102, and a second plane-convex cylindrical lens which is coaxial with the collimating lens 102, wherein the first plane-convex cylindrical lens and the second plane-convex cylindrical lens are mutually vertically matched, focused and compressed light spots are rectangular light spots, convex surfaces of the first plane-convex cylindrical lens and the second plane-convex cylindrical lens are used as light incidence surfaces, and a plane is used as a light emergence surface.
The flow chamber 105 is provided with a liquid pipeline 120, and the liquid pipeline 120 is arranged on the optical axis and is positioned at a rectangular light spot formed by matching, focusing and compressing the first and second convex cylindrical lenses.
When the device is used, laser emitted by the semiconductor laser 101 is changed into parallel light under the action of the collimating lens 102, the parallel light passes through the first plano-convex cylindrical lens 103 and the second plano-convex cylindrical lens 104, light spots of the parallel light are compressed into rectangular light spots 121 and are focused to the center of the flow chamber 105, preferably, a focal line intersects with a central axis of the liquid path pipeline 120 to form a light sensitive area 123, sample particles 122 to be detected pass through the light sensitive area 123 through a liquid path system, and the particles are scattered. Intrinsic fluorescence is also emitted in the case of microbial particles.
The scattered light receiving system is arranged on the opposite side of the laser emission system and used for receiving scattered light generated by sample particles to be detected in the liquid path pipeline 120, the scattered light receiving system comprises a diaphragm 106, a first aspheric lens 107, a long-wave cut-off filter 108, a first plano-convex lens 109 and a scattered light detector 110 which are sequentially arranged on the same optical axis, the convex surface of the first plano-convex lens 109 is a light incidence surface, the plane of the first plano-convex lens is a light emergence surface, and the scattered light detector 110 is connected with a scattered light preamplifier 115.
Preferably, the stop 106 is provided at the center of the light incident surface of the first aspherical lens 107. Scattered light generated at the position of the light sensitive area 123 of the liquid channel pipeline 120 is blocked by the diaphragm 106 arranged on the optical axis to directly irradiate the direct light on the first aspheric lens 107, so that the scattered light receiving system only receives the scattered light, residual light of the scattered light blocked by the diaphragm 106 is changed into parallel light through the first aspheric lens 107, the scattered light is obtained under the action of the long wave cut-off filter 108, the parallel light is collected by the first plano-convex lens 109 and then received by the scattered light detector 110 to be converted into a current signal, the current signal is weak, and the photocurrent is converted into voltage and amplified through the scattered light preamplifier 115 to be converted into a detectable signal.
The fluorescence receiving system is arranged on one side of the laser emission system and used for receiving fluorescence generated by sample particles to be detected in the liquid pipeline, the optical axis of the fluorescence receiving system is perpendicular to that of the laser emission system, the fluorescence receiving system comprises a second aspheric lens 111, a short-wave cut-off filter 112, a second plano-convex lens 113 and a fluorescence detector 114, the second aspheric lens 111, the short-wave cut-off filter 112, the second plano-convex lens 113 and the fluorescence detector 114 are sequentially arranged on the same optical axis, the convex surface of the second plano-convex lens 113 is a light incidence surface, the plane of the second plano-convex lens is a light emission surface, and the fluorescence detector 114 is connected with a fluorescence preamplifier 116.
If the microorganism particles exist, intrinsic fluorescence emitted from the position, located in the light sensitive area 123, of the liquid pipeline 120 is changed into parallel light through the second aspheric lens 111, the parallel light is obtained under the action of the short-wave cut-off filter 112, the parallel light is converged through the second plano-convex lens 113 and then absorbed by the fluorescence detector 114 to be converted into a current signal, the current signal is extremely weak, and photocurrent is converted into voltage and amplified through the fluorescence preamplifier 116 to be converted into a detectable signal.
If the particles are not microbial particles, no fluorescence signal is received.
The signal processing system comprises a filter 117, an A/D converter 118 and an upper computer 119 which are connected in sequence, wherein the filter 117 is connected with a scattered light preamplifier 115 and a fluorescence preamplifier 116.
The electric signal output by the photoelectric device is mixed with a plurality of high-frequency noises, so the signal amplified by the scattered light preamplifier 115 is sent to the filter 117 to filter the high-frequency noises in the signal, then the signal is converted into a digital signal through the A/D converter 118, and the digital signal is sent to the upper computer 119 through the communication interface to be analyzed, displayed and the like, the particle size of each particle can be calculated, and finally the number of the particles in each particle size section is obtained.
The electric signal output by the photoelectric device is mixed with a plurality of high-frequency noises, so that the signal amplified by the fluorescent preamplifier 116 is sent to the filter 117 to filter the high-frequency noises in the signal, then the signal is converted into a digital signal by the A/D converter 118, and the digital signal is sent to the upper computer 119 by the communication interface to be analyzed, displayed and the like, so that the fluorescence intensity information emitted by the microbial particles can be obtained, and the microbial particles can be simply classified and counted.
The minimum detection resolution of the liquid pipeline 120 is one microorganism particle; the scattered light detector 110 is a photodiode; the fluorescence detector 114 is an avalanche photodiode or a photomultiplier tube.
A405 nm semiconductor laser can effectively detect bioactive substances NADH, riboflavin and the like and induce the bioactive substances to excite fluorescence. The semiconductor laser 101 preferably emits 405nm laser light.
In order to facilitate understanding of the structure, the utility model also discloses an online detection method for microorganisms in water, which comprises the following steps:
The laser emission system is arranged, laser is generated by the semiconductor laser 101, a rectangular light spot is generated by focusing and compressing, and the light spot is irradiated on the liquid pipeline 120 of the flow chamber 105, so that the measured particles are scattered or/and intrinsic fluorescence is generated.
A scattered light receiving system is arranged on the opposite side of the laser emission system, scattered light generated by irradiated sample particles to be detected is received, a scattered light processing component of the scattered light receiving system is arranged to be coaxial with the laser emission system, the scattered light processing component receives the scattered light and converges the scattered light, the scattered light detector 110 receives the scattered light converged by the scattered light processing component and converts the scattered light into a current signal, and the converted current signal is amplified into a detectable voltage signal through a scattered light preamplifier 115 connected with the scattered light detector 110.
A fluorescence receiving system is arranged on one side of the laser emitting system and perpendicular to an optical axis of the laser emitting system, and receives intrinsic fluorescence generated by the irradiated sample particles to be detected; the optical axis of the fluorescence light processing component of the fluorescence receiving system is perpendicular to the optical axis of the laser emitting system, the fluorescence light processing component receives and converges fluorescence, and the fluorescence collected by the fluorescence light processing component is absorbed by the fluorescence detector 114 and converted into a current signal, and the current signal is amplified into a detectable voltage signal by the fluorescence preamplifier 116 connected to the fluorescence detector 114.
A signal processing system is arranged, the voltage signal amplified by the scattered light preamplifier 115 and the voltage signal amplified by the fluorescence preamplifier 116 are received, high-frequency noise in the signals is filtered by a filter 117, the signals are converted into digital signals through an A/D converter 118 and transmitted to an upper computer 119, the digital signals are analyzed, displayed and the like by the upper computer 119, the particle size of each particle is calculated, and the number of the particles in each particle size section is finally obtained; or the fluorescence intensity information emitted by the microorganism particles can be obtained, so that the microorganism particles can be simply classified and counted.
Preferably, the central axis of the liquid channel 120 of the flow chamber 105 intersects with the focal line of the rectangular light spot generated by focusing and compressing of the laser emission system to form a light sensitive area, and the sample particles to be detected pass through the light sensitive area through the liquid channel 120 to scatter or/and generate intrinsic fluorescence.
The scattered light processing component comprises a diaphragm 106, a first aspheric lens 107, a long-wave cut-off filter 108, a first plano-convex lens 109 and a scattered light detector 110 which are sequentially arranged along the same optical axis, wherein the convex surface of the first plano-convex lens 109 is a light incidence surface, and the plane of the first plano-convex lens 109 is a light emergence surface.
The fluorescence light processing component comprises a second aspheric lens 111, a short-wave cut-off filter 112, a second plano-convex lens 113 and a fluorescence detector 114 which are sequentially arranged along the same optical axis, wherein the convex surface of the second plano-convex lens 113 is a light incidence surface, and the plane of the second plano-convex lens 113 is a light emergence surface.
Claims (5)
1. The utility model provides an aquatic microorganism on-line measuring device which characterized in that: the laser emitting system comprises a semiconductor laser, a collimating lens, a first flat convex cylindrical lens with the same optical axis as the collimating lens and a second flat convex cylindrical lens with the same optical axis as the collimating lens, wherein the first and second convex cylindrical lenses are vertically matched with each other, focused and compressed light spots are rectangular light spots, convex surfaces of the first and second convex cylindrical lenses are used as light incident surfaces, and a plane is used as a light emergent surface; the flow chamber is provided with a liquid pipeline, and the liquid pipeline is arranged on the optical axis and is positioned at a rectangular light spot formed by the matching, focusing and compressing of the first and second convex cylindrical lenses; the scattered light receiving system is arranged on the opposite side of the laser emission system and used for receiving scattered light generated by sample particles to be detected in a liquid path pipeline, and comprises a diaphragm, a first aspheric lens, a long-wave cut-off filter, a first plano-convex lens and a scattered light detector which are sequentially arranged along the same optical axis, wherein the convex surface of the first plano-convex lens is a light incidence surface, the plane of the first plano-convex lens is a light emergence surface, and the scattered light detector is connected with a scattered light preamplifier; the fluorescence receiving system is arranged on one side of the laser emission system and used for receiving fluorescence generated by sample particles to be detected in the liquid pipeline, the optical axis of the fluorescence receiving system is perpendicular to that of the laser emission system, the fluorescence receiving system comprises a second aspheric lens, a short-wave cut-off filter, a second plano-convex lens and a fluorescence detector which are sequentially arranged along the same optical axis, the convex surface of the second plano-convex lens is a light incidence surface, the plane of the second plano-convex lens is a light emission surface, and the fluorescence detector is connected with a fluorescence preamplifier; the signal processing system comprises a filter, an A/D converter and an upper computer which are connected in sequence, wherein the filter is connected with a scattered light preamplifier and a fluorescence preamplifier.
2. The in-water microorganism on-line detection device of claim 1, wherein: the central axis of the liquid pipeline is intersected with the optical axis.
3. The in-water microorganism on-line detection device of claim 1, wherein: the semiconductor laser emits 405nm laser.
4. The in-water microorganism on-line detection device of claim 1, wherein: the diaphragm is arranged at the center of the light incidence surface of the first aspheric lens.
5. The in-water microorganism on-line detection device of claim 1, wherein: the minimum detection resolution of the liquid pipeline is one microorganism particle; the scattered light detector is a photodiode; the fluorescence detector is an avalanche photodiode or a photomultiplier tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221862076.1U CN218239728U (en) | 2022-07-18 | 2022-07-18 | Aquatic microorganism on-line measuring device |
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| CN202221862076.1U CN218239728U (en) | 2022-07-18 | 2022-07-18 | Aquatic microorganism on-line measuring device |
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| CN218239728U true CN218239728U (en) | 2023-01-06 |
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| CN202221862076.1U Active CN218239728U (en) | 2022-07-18 | 2022-07-18 | Aquatic microorganism on-line measuring device |
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