CN218213603U - Imaging component bearing device, base station and microscopic image acquisition device - Google Patents

Abstract

The imaging component bearing device, the base station and the microscopic image acquisition device comprise an imaging lens bracket, an imaging lens clamping seat and an adjusting guide seat; the bottom of the imaging lens clamping seat is movably clamped between the imaging lens bracket and the adjusting guide seat; the levelness of the upper surface of the bottom of the imaging lens clamping seat relative to the bottom of the imaging lens support can be adjusted until a microscopic imaging assembly fixed on the imaging lens clamping seat is orthogonal to the upper surface of the bottom of the imaging lens clamping seat, and the microscopic imaging assembly is fixed on the imaging lens clamping seat. With the help of imaging components bearing device's structural design to combine spiral fastener, make apart from the regulation fastener can promote the precision of distance adjustment, make the regulation of the contained angle alpha angle between imaging components and the ideal optical axis more meticulous, thereby the removal distance in the optical axis direction is more meticulous apart from L2's regulation promptly, realizes the optical adjustment process of high accuracy.

Description

Imaging component bearing device, base station and microscopic image acquisition device

Technical Field

The application belongs to the technical field of microscopic image acquisition, and particularly relates to an imaging component bearing device and a base platform for bearing a microscopic image acquisition device, and the microscopic image acquisition device arranged on the imaging component bearing base platform.

Background

In the prior art, microscope imaging usually requires manual microscope focus adjustment to obtain a clear target image. In the microscopic imaging process of improved automatic focusing by using an electronic control system, the process of adjusting the focal length is a very precise adjusting and controlling process due to different microscope magnification factors, so that the precision requirement on a power part in the microscopic imaging process is very high, and the precision requirement on a mechanical bearing mechanism for bearing the power part and a key imaging component is also very high.

When the magnification is 40 times, the distance deviation between the objective lens focus and the target object, namely the deviation allowed by the focus depth, is about plus or minus 0.25um (micrometer), and at the moment, the resolution of the distance adjustment movement between the objective lens and the target imaging object can ensure the adjustment reliability in the electric control microscopic imaging focusing process only by achieving the same number of levels of the imaging focusing depth of field. That is, if the imaging depth of field of the microscope is 2um to 3um, the degree of 2um to 3um is required to be achieved for the moving precision of the electric control mechanical platform for bearing the movement of the microscope, so that the microscopic imaging device can stably obtain clear microscopic method images in the moving process.

The moving process of the electric control mechanical platform for bearing the microscope to move requires the plane precision to be 2um to 3um, on one hand, the driving control precision of the motor is required, and meanwhile, higher requirements are provided for a base station for bearing the microscope. In order to have a proper magnification, the microscope is generally in a long cylinder shape, and a lens combination is arranged behind an objective lens to adjust light, and the central optical axis of the lens combination needs to be orthogonal with an imaging target object with high precision; if the included angle between the central optical axis and the plane of the imaging target object deviates and the slight included angle between the central optical axis and the plane of the imaging target object also causes a larger deviation of the distance between the imaging target object and the focal point, when the length of the optical axis of the lens combination is larger, the non-orthogonal influence between the central optical axis and the plane of the imaging target object is larger, and the unclear and ghost images of the images can be presented in the microscopic images.

However, the prior art has an exponential increase in the machining accuracy requirements and the machining cost of the mechanical parts; namely, the processing cost of the high-precision mechanical plane is far greater than that of the common precision. How to realize high-precision application requirements by using a general-precision mechanical plane is one of the key technical problems to be solved by the application.

Even a high-precision part has some machining dimension errors, and how to ensure that a central optical axis and a plane where an imaging target object is located are as close to orthogonal as possible in microscopic imaging under the condition that the part has the dimension errors is also one of the key technical problems to be solved by the application.

Disclosure of Invention

The technical problem to be solved by the application is to avoid the defect that a plane where a central optical axis and an imaging target object are located may have a non-orthogonal condition in the prior art, and provide an imaging component bearing device, a base station and a microscopic imaging acquisition device arranged on the imaging component bearing base station.

The technical scheme for solving the problems is that the imaging component bearing device is used for bearing an imaging component in a microscopic imaging acquisition device; the imaging lens clamping device comprises an imaging lens bracket, an imaging lens clamping seat and an adjusting guide seat; the adjusting guide seat is fixedly connected with the imaging lens bracket; the imaging lens clamping seat comprises an imaging lens clamping seat bottom and a lens clamping part; the lens through hole of the imaging lens clamping seat passes through the bottom of the imaging lens clamping seat and the lens clamping part; a bracket lens through hole is formed in the imaging lens bracket; a guide seat through hole is formed in the adjusting guide seat; the lens clamping part passes through a guide seat through hole on the adjusting guide seat, so that the bottom of the imaging lens clamping seat is movably clamped between the imaging lens bracket and the adjusting guide seat; when the bottom of the imaging lens clamping seat is clamped between the imaging lens support and the adjusting guide seat, the positions of the support lens through hole and the imaging lens clamping seat lens through hole correspond.

The imaging lens support comprises an imaging lens support bottom; the bracket lens through hole is arranged on the bottom of the imaging lens bracket; the bottom of the imaging lens bracket is provided with at least three first elastic supporting pieces which are uniformly distributed by taking the bracket lens through hole as the center; the elastic supporting surface or the elastic supporting point of the first elastic supporting piece faces upwards and is used for supporting the bottom of the imaging lens clamping seat.

At least three limiting position adjusting holes are formed in the adjusting guide seat, and limiting distance adjusting fasteners are arranged in the limiting position adjusting holes; the position of each limiting position adjusting hole corresponds to the position of each first elastic supporting piece.

The limiting position adjusting hole is a limiting position adjusting through hole; the limiting distance adjusting fastener penetrates through the limiting position adjusting through hole to be in contact with the bottom of the imaging lens clamping seat.

The limiting distance adjusting fastener is a spiral fastener, and the spiral fastener controls the downward distance of the spiral fastener through the rotation angle; the lead S is the descending distance of the screw fastener when the screw rotates for one circle; the lead S of the screw-type fastener ranges from 0.2mm to 1mm.

Two position fixing through holes A1 are symmetrically arranged on two sides of each limiting position adjusting hole on the adjusting guide seat; correspondingly, two position fixing through holes B1 are also formed in the bottom of the imaging lens clamping seat; and each position fixing fastener sequentially passes through the corresponding fixing through hole A1 and the corresponding fixing through hole B1 to fixedly adjust the relative position between the guide seat and the bottom of the imaging lens clamping seat.

When the bottom of the imaging lens clamping seat is pressed on the first elastic supporting piece, the descending distance range of the first elastic supporting piece is 0.3mm to 2mm.

The adjusting guide seat is provided with at least three second elastic supporting pieces for radially supporting the bottom of the imaging lens clamping seat; the second elastic supporting pieces are uniformly distributed by taking the geometric center of the adjusting guide seat as a center, and the second elastic supporting pieces are radially arranged by taking the lens through hole of the imaging lens clamping seat as a center; the elastic supporting surface or the elastic supporting point of the second elastic supporting piece faces to the radial center of the lens through hole of the imaging lens clamping seat and is used for contacting with the side wall of the bottom of the imaging lens clamping seat.

The imaging component bearing device also comprises a motor bracket and a movable plate; the motor bracket is used for fixing a motor for driving the imaging component to move; the movable plate is used for being connected with a driving force output device of the motor; the imaging lens support is fixedly connected with the movable plate, and the imaging lens support acquires moving power by means of the movable plate.

The imaging component bearing device also comprises a motor main body and a driving force output device; the motor main body is fixed on the motor bracket; the rotating shaft of the motor main body is connected with a driving force output device; the driving force output device is connected with the movable plate to drive the movable plate to move.

The imaging lens support also comprises an imaging lens support vertical surface and two imaging lens support supporting half side vertical surfaces; the imaging lens support is fixedly connected with the movable plate through the imaging lens support vertical surface, so that the imaging lens support can move along with the movable plate; the bottom of the imaging lens support and the vertical face of the imaging lens support are fixedly connected through two supporting half side vertical faces of the imaging lens support.

The technical solution for solving the above problems can also be an imaging component bearing base station, which is used for bearing the microscopic imaging acquisition device, and includes the imaging component bearing device; the imaging target clamping assembly comprises a first bearing support, a second bearing support and an imaging target clamping assembly; the first bearing support and the second bearing support are respectively arranged on the bearing substrate; the first bearing bracket is used for bearing the imaging component bearing device; the second bearing support is used for bearing the imaging target clamping assembly, so that the imaging target clamping assembly is arranged below the imaging assembly bearing device; the imaging component bearing device is fixed on the transverse supporting arm by virtue of the transverse supporting arm fixing through hole; the second bearing support is provided with a second supporting through hole, and the central position of the transverse supporting arm fixing through hole and the central position of the second supporting through hole are relatively fixed.

The outward extending parts of the transverse supporting arms are connected with the longitudinal supporting columns in an enhanced mode through reinforcing corner connectors.

The bottom of the bearing substrate is provided with a plurality of shockproof damping support legs.

The imaging target clamping assembly comprises an XY axis moving platform assembly and a reagent card clamping seat assembly; the reagent card clamping seat assembly is fixed at the top of the XY axis moving platform assembly; and the XY-axis moving platform assembly is fixed on the top of the second bearing support.

The reagent card clamping seat assembly comprises a reagent card clamping seat main body and a reagent card clamping part; the reagent card clamping part is used for clamping a reagent card inserted from the outside; the bottom surface of the reagent card clamping seat main body is parallel to the bottom surface of the reagent card clamping part.

And a plurality of third elastic supporting pieces are arranged on the second bearing support and are used for adjusting the levelness of the bottom surface of the XY-axis moving platform assembly.

And a plurality of fourth elastic supporting pieces are arranged on the top surface of the XY-axis moving platform assembly and used for adjusting the levelness of the bottom surface of the reagent card clamping seat assembly.

The imaging assembly bears the base station, still include the light source assembly; the second bearing bracket is provided with a light source fixing circular truncated cone by taking the second supporting through hole as a center; the light source component is fixed on the light source fixing round platform; the emergent light of the light source in the light source component and the imaging lens in the imaging component bearing device are on the same imaging optical axis.

The technical solution for solving the above problems may also be a microscopic image obtaining apparatus, including the above imaging component bearing apparatus, or the above imaging component bearing base; the microscope imaging assembly is fixed on the imaging lens clamping seat.

The technical solution for solving the above problems may also be an adjusting method of a microscopic image obtaining apparatus, based on the imaging component carrying apparatus; and a microscopic imaging assembly fixed on the imaging lens holder; the microscopic imaging assembly comprises an objective lens; the objective lens passes through the imaging lens clamping seat, the objective lens is clamped and fixed by the imaging lens clamping seat, and one end of the objective lens is positioned below the plane where the bottom of the imaging lens clamping seat is positioned; the target imaging optical axis passes through the objective lens; the method comprises the following steps: step A: b, acquiring the offset direction of the lens through hole of the imaging lens holder and the target imaging optical axis, and entering the step B if the offset included angle between the plane where the lens through hole of the imaging lens holder is located and the target imaging optical axis is larger than a set target value; and B: selecting a limiting distance adjusting fastener in a limiting position adjusting hole closest to the end part of the objective lens, adjusting the downward passing depth of the limiting distance adjusting fastener, and adjusting the force of the bottom of the imaging lens clamping seat pressing the corresponding first elastic supporting piece at the point position; step C: checking the offset included angle between the plane of the lens through hole of the imaging lens clamping seat and the target imaging optical axis again, and returning to the step B if the offset included angle is larger than the set target value; until the offset included angle between the plane of the lens through hole of the imaging lens clamping seat and the target imaging optical axis is smaller than or equal to the set target value.

The adjusting method of the microscopic image acquisition device further comprises the following steps: and when the offset included angle between the plane of the lens through hole of the imaging lens clamping seat and the target imaging optical axis is smaller than or equal to the set target value, the imaging lens clamping seat bottom and the adjusting guide seat are fixedly connected.

Compared with the prior art, the bottom of the imaging lens clamping seat is movably clamped between the imaging lens support and the adjusting guide seat; the adjustable space is arranged between the bottom of the imaging lens clamping seat and the imaging lens support, and the imaging component clamped on the imaging lens clamping seat, especially the lens component, has the opportunity of adjusting the verticality of the optical axis.

Compared with the prior art, two, three first elastic support piece of the beneficial effect of this application to support lens through-hole sets up as central evenly distributed, and its elastic support face or elastic support point up support imaging lens holder bottom, and the holding surface that three point supported is movable adjustable, can adjust the levelness of holding surface in order to offset the holding surface levelness deviation that relevant mechanical parts machining error leads to through the adjustment of three point pressfitting dynamics.

Compared with the prior art, the third of the beneficial technical effect of this application, spacing distance adjusting fastener can directly carry out the pressfitting dynamics adjustment of single-point, provides more refined adjustment means for the adjustment of holding surface levelness deviation, and festival fastener can be the spacing distance adjusting fastener of high accuracy.

Compared with the prior art, the fourth beneficial technical effect of this application, spacing distance adjusting fastener pass through spacing position adjusting through-hole and the upper surface direct contact of imaging lens holder bottom, adjusts the pressure of adjusting the guide holder pressfitting on corresponding first elastic support piece to adjust the down distance of imaging lens holder bottom pressfitting on first elastic support piece.

Compared with the prior art, the imaging lens clamping seat has the advantages that the position fixing fastener sequentially penetrates through the corresponding fixing through hole A1 and the corresponding fixing through hole B1, and the relative position between the guide seat and the bottom of the imaging lens clamping seat is fixedly adjusted; namely, after each first elastic supporting piece is adjusted in place, the relative position between the fixed adjusting guide seat and the bottom of the imaging lens clamping seat can be fixed.

Compare with prior art, six of the beneficial technical effect of this application, when formation of image lens grip slipper bottom pressfitting was on first elastic support piece, first elastic support piece's down distance scope was 0.3mm (millimeter) to 2mm, and this distance is enough to be used for offsetting the holding surface level deviation that relevant mechanical parts machining error leads to.

Compare with prior art, seven of the beneficial technological effect of this application, the at least three second elastic support piece who sets up on adjusting the guide holder guarantees imaging lens holder's centering nature, reduces the frictional force of adjusting between guide holder and the imaging lens holder.

Compare with prior art, eight of the beneficial technical effect of this application, the setting of fly leaf makes the imaging lens support acquire removal power with the help of the fly leaf. The movable plate can drive the imaging lens support to longitudinally move along the direction of the optical axis.

Compare with prior art, nine of the beneficial technical effect of this application, imaging lens support erects the face and two imaging lens support half side facades make imaging lens support bottom support imaging lens holder more firmly and adjust the guide holder.

Compare with prior art, ten of the beneficial technological effect of this application, horizontal support arm fixing hole central point puts and second support through hole central point puts relatively fixed, for follow-up light source subassembly, formation of image target centre gripping subassembly, formation of image subassembly bearing device in corresponding optical component's the coaxial basis that provides.

Compare with prior art, eleven of the beneficial technological effect of this application, a plurality of damping stabilizer blades that take precautions against earthquakes have further strengthened the mechanism stability and the robustness that imaging assembly bore the base station, have reduced the influence that outside vibrations bore the base station to imaging assembly.

Compare with prior art, the twelve of the beneficial technical effect of this application, the bottom surface of reagent card grip slipper main part and the bottom surface of reagent card clamping part are parallel for the reagent card can be steadily by the centre gripping of reagent card clamping part and can with the imaging optical axis quadrature.

Compared with the prior art, thirteen of the beneficial technical effect of this application, XY axle moving platform subassembly can drive reagent card holder subassembly and be horizontal two-dimensional movement, has increased the flexibility of system, conveniently adjusts the formation of image target area.

Compare with prior art, the fourteen of the beneficial technical effect of this application, third elastic support spare can partially offset the assembly error between XY axle moving platform subassembly bottom surface and the second bears the weight of the support top surface, can adjust the levelness of XY axle moving platform subassembly bottom surface. The levelness of the bottom surface of the XY-axis moving stage assembly is a levelness when orthogonal to the optical axis.

Compare with prior art, fifteen of the beneficial technical effect of this application, fourth elastic support piece can partially offset the assembly error between XY axle moving platform subassembly top surface and the reagent card holder subassembly bottom surface, can adjust the levelness of reagent card holder subassembly bottom surface. The reagent card holder assembly has a bottom surface with a horizontal degree orthogonal to the optical axis.

Compare with prior art, sixteen of the beneficial technological effects of this application, with the help of the structural design that imaging assembly bore device to combine spiral fastener, make apart from adjusting the precision that the fastener can be with distance adjustment promote, make the regulation of the contained angle alpha angle between imaging assembly and the ideal optical axis more meticulous, the removal distance in the optical axis direction is more meticulous apart from L2's regulation promptly. In the balance between the machining precision and the cost of the component, the dimensional adjustment with higher fineness can be realized with relatively lower machining precision, and the method is an efficient and low-cost solution. The high-precision position adjustment can be realized by the low-precision part machining size, and the distance adjustment on the mm size between the bottom of the imaging lens clamping seat and the adjusting guide seat can be converted into the distance adjustment on the finer size of the objective lens in the optical axis direction. Namely, the distance adjustment on the mm size between the bottom of the imaging lens clamping seat and the adjusting guide seat can be converted into finer distance adjustment of the objective lens in the optical axis direction; the lens in the imaging component bearing device and the imaging optical axis where the lens is located can be conveniently adjusted to the position orthogonal to the plane where the lens through hole of the imaging lens clamping seat is located, so that the imaging optical axis can be orthogonal to a reagent card clamped on the reagent card clamping seat component when a target area is imaged, and the high quality of a microscopic imaging image can be guaranteed.

Drawings

FIG. 1 is a schematic view of an embodiment of an imaging assembly carrier 200 in an assembled state;

FIG. 2 is a schematic exploded view of an embodiment of an imaging assembly carrier 200;

fig. 3 is a schematic bottom view of the combination of the imaging lens holder 260 and the adjustment guide 270;

FIG. 4 is a schematic view of a second embodiment of an imaging assembly carrier 200 in an assembled state;

FIG. 5 is a schematic diagram of a second embodiment of an imaging assembly carrier 200 in an exploded state;

FIG. 6 is a second exploded view of the second embodiment of the imaging assembly carrier 200;

FIG. 7 is a schematic top front view of a second embodiment of an imaging assembly carrier 200;

FIG. 8 is a schematic cross-sectional view of AA of FIG. 7;

FIG. 9 is a schematic view of an assembled state of one embodiment of a microscopic image acquisition apparatus;

FIG. 10 is a schematic view of one embodiment of an imaging assembly carrying base in an assembled state;

FIG. 11 is a schematic view of an imaging assembly carrying base and light source assembly in an exploded state;

FIG. 12 is a schematic view of an exploded state of imaging target holding assembly 300;

FIG. 13 is a schematic view of a reagent card holder assembly 320;

FIG. 14 is a schematic view of the reagent card holder assembly 320 in an exploded state;

figure 15 is a schematic view of the XY axis motion stage assembly 310;

fig. 16 is an exploded view of the XY-axis motion stage assembly 310;

fig. 17 is a schematic view showing a state where the imaging lens holder 260, the adjustment guide 270 and the imaging lens holder base 232 are assembled;

fig. 18 is a schematic top view of the combined state of the imaging lens holder 260, the adjustment guide 270 and the imaging lens holder base 232;

FIG. 19 is a schematic cross-sectional view of BB of FIG. 18; reference numeral F in the figure denotes a contact surface F of the lower surface of the imaging lens holder base 261 and the first elastic support 233; reference G indicates an imaging optical axis; in an ideal state, the imaging optical axis G is perpendicular to the contact surface F, which means that the imaging optical axis is orthogonal to the imaging target area, a plane image of the target imaging area can be normally microscopically amplified, the image is clear, and no ghost image exists;

FIG. 20 is a partially enlarged view of one of the portions K of FIG. 19; h1 in the drawing is a gap from the lower surface of the imaging lens holder bottom 261 to the upper surface of the imaging lens holder bottom 232;

FIG. 21 is a second enlarged partial view of the portion K in FIG. 19; h2 in the drawing is a gap from the lower surface of the imaging lens holder bottom 261 to the upper surface of the imaging lens holder bottom 232; gap H2 is less than gap H1;

FIG. 22 is one of the simplified schematic diagrams of FIG. 19;

fig. 23 is a second simplified schematic diagram of fig. 19.

Detailed Description

Embodiments of the present application will be described in further detail below with reference to the drawings.

It should be noted that the following description is of the preferred embodiments of the present application and should not be construed as limiting the present application in any way. The description of the preferred embodiments of the present application is made only as an illustration of the general principles of the application. The embodiments described in this application are only some embodiments of the invention and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the description of the present application, it is to be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the present application and for simplicity in description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be operated in a particular manner, and thus should not be considered limiting. Furthermore, the terms "first", "second", and technical features numbered with Arabic numerals 1, 2, 3, etc., and such numbers as "A" and "B" are used for descriptive purposes only and are not intended to represent temporal or spatial order; are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first," "second," and numbered with an Arabic numeral 1, 2, 3, etc., may explicitly or implicitly include one or more of the features. In the description of the present application, "a number" means two or more unless specifically limited otherwise.

As shown in fig. 1-2, an embodiment of an imaging assembly carrier 200 includes an imaging lens support 230, an imaging lens holder 260, and an adjustment guide 270; the adjusting guide 270 is fixedly connected with the imaging lens bracket 230; the imaging lens holder 260 includes an imaging lens holder bottom 261 and a lens holder 262; the imaging lens holder lens through hole 266 passes through the imaging lens holder bottom 261 and the lens holder 262; a holder lens through hole 231 is provided on the imaging lens holder 230; the adjusting guide 270 is provided with a guide through hole 279; the lens holder 262 passes through the guide holder through-hole 279 on the adjustment guide holder 270, so that the imaging lens holder bottom 261 is movably clamped between the imaging lens holder 230 and the adjustment guide holder 270; when imaging lens holder bottom 261 is snapped between the imaging lens holder and the adjustment guide, holder lens through-hole 231 corresponds to imaging lens holder lens through-hole 266.

In the embodiment of the imaging assembly carrier 200 shown in fig. 1-3, the imaging lens support 230 includes an imaging lens support base 232; the holder lens through hole 231 is provided on the imaging lens holder bottom 232; at least three first elastic supporting pieces 233 are arranged on the bottom 232 of the imaging lens bracket, and the plurality of first elastic supporting pieces 233 are uniformly distributed by taking the bracket lens through hole 231 as the center; the elastic support surface or point of the first elastic support 233 faces upward for supporting the imaging lens holder bottom 261. A first elastic support member counterbore 234 is formed in the imaging lens support bottom 232, the first elastic support member counterbore 234 is used for accommodating the first elastic support member 233, and the bottom end of the first elastic support member 233 falls into the first elastic support member counterbore 234 for support. The number and location of the first resilient support counterbores 234 correspond one-to-one with the first resilient supports 233; first resilient support counterbore 234 is not a through bore.

In the embodiment of the image forming assembly carrier 200 shown in fig. 1 to 3, at least three limiting position adjusting holes 271 are formed in the adjusting guide 270, and limiting distance adjusting fasteners 272 are disposed in the limiting position adjusting holes 271; the position of each limit position adjusting hole 271 corresponds to the position of each first elastic support 233. By adjusting the distance of the limit distance adjusting fastener 272 penetrating into the limit position adjusting hole 271, the pressure of the adjusting guide seat 270 pressing on the corresponding first elastic support 233 at that point is adjusted.

In the embodiment of the imaging assembly carrier device 200 shown in fig. 1 to 3, the limit position adjusting hole 271 is a limit position adjusting through hole; the limit distance adjusting fastener 272 passes through the limit position adjusting through hole to contact the imaging lens holder bottom 261. The limiting distance adjusting fastener 272 passes through the limiting position adjusting through hole 271 to directly contact with the upper surface of the imaging lens holder bottom 261, so as to adjust the pressing force of the adjusting guide seat 270 on the corresponding first elastic support 233, thereby adjusting the downward distance of the imaging lens holder bottom 261 on the first elastic support 233.

In the embodiment of the image forming module carrying device 200 shown in fig. 1 to 2, two position fixing through holes a1275 are symmetrically formed on two sides of each limit position adjusting hole 271 on the adjusting guide 270; accordingly, two position fixing through holes B1265 are also provided on the imaging lens holder bottom 261; each of the position fixing fasteners passes through the corresponding fixing through hole a1275 and the fixing through hole B1265 in turn, and fixes the relative position between the adjustment guide 270 and the imaging lens holder bottom 261. The position fixing fasteners are not shown in the drawings. When the imaging lens holder bottom 261 is pressed on the first elastic supporting member 233, the descending distance of the first elastic supporting member 233 ranges from 0.3mm to 2mm or from 0.5mm to 1.2mm.

In the embodiment of the imaging assembly carrier device 200 shown in fig. 1 to 3, at least three second elastic supports 276 are provided on the adjustment guide 270 for radial support of the imaging lens holder base 261; the second elastic supporting members 276 are uniformly distributed around the geometric center of the adjusting guide seat 270, and the second elastic supporting members 276 are radially arranged around the lens through hole 266 of the imaging lens holder; the resilient support surface or point of resilient support 276 is directed toward the radial center of imaging lens holder lens throughbore 266 for contact with the sidewall of imaging lens holder base 261. At least three second elastic supporting pieces 276 arranged on the adjusting guide seat 270 ensure the centering performance of the imaging lens clamping seat 260 and reduce the friction force between the adjusting guide seat 270 and the imaging lens clamping seat 260.

In the embodiment of the imaging assembly carrier 200 shown in fig. 1-4, the imaging lens frame 230 further includes an imaging lens frame riser 235 and two imaging lens frame support half-risers 236; the imaging lens support 230 is fixedly connected with the movable plate 220 through the imaging lens support vertical surface 235, so that the imaging lens support 230 can move along with the movable plate 220; the imaging lens support base 232 and the imaging lens support riser 235 are fixedly connected by two imaging lens support half-sides risers 236.

In the embodiment of the image forming assembly carrying device 200 shown in fig. 4 to 8, the device further includes a motor bracket 210, a movable plate 220, a motor main body 280 and a driving force output device 290; the motor bracket 210 is used for fixing a motor main body 280 for driving the imaging component to move; the motor main body 280 is fixed on the motor bracket 210; the rotational shaft of the motor main body 280 is connected to the driving force output device 290; the driving force output device 290 is connected to the movable plate 220 to drive the movable plate 220 to move. The movable plate 220 is used for being connected with a driving force output device 290 of the motor main body 280; the imaging lens support 230 is fixedly connected with the movable plate 220, and the imaging lens support 230 obtains moving power by means of the movable plate 220.

In the embodiment of the imaging assembly carrier 200 shown in fig. 4 to 8, the driving force output device 290 includes a bearing housing assembly 291, a ball screw nut 292, a ball screw nut fastening housing 293, and a ball screw 294; the bearing seat assembly 291 is sleeved on a rotating shaft of the motor, and the ball screw 294 is connected with the rotating shaft of the motor; a ball screw nut 292 is sleeved on the ball screw 294; the ball screw nut fastening seat 293 is connected to the ball screw nut 292, and the ball screw nut fastening seat 293 is fixedly connected to the movable plate 220 and moves along with the movement of the ball screw nut 292.

As shown in fig. 9 to 11, an embodiment of an imaging component carrying base station for carrying a microscopic imaging acquisition apparatus includes an imaging component carrying device 200, a carrying substrate 120, a first carrying bracket 110, a second carrying bracket 130, and an imaging target holding component 300; the first and second carrier supports 110 and 130 are respectively disposed on the carrier substrate 120; the first carrying bracket 110 is used for carrying the imaging component carrying device 200; the second carrying bracket 130 is used for carrying the imaging target holding assembly 300, so that the imaging target holding assembly 300 is arranged below the imaging assembly carrying device 200; the bottom of the carrier substrate 120 is provided with a plurality of anti-vibration damping legs 125.

As shown in fig. 9 to 11, in an embodiment of an imaging component carrying base station for a microscopic imaging acquisition apparatus, a first carrying bracket 110 includes a longitudinal supporting column 111 and a transverse supporting arm 112, one end of the longitudinal supporting column 111 and one end of the transverse supporting arm 112 are fixedly connected, and an outward extending portion 1122 of the transverse supporting arm is connected with the longitudinal supporting column 111 in an enhanced manner through a reinforcing corner brace 113; a transverse support arm fixing through hole 1123 is formed in the transverse support arm outward extension portion 1122, and the imaging assembly carrier device 200 is fixed to the transverse support arm 112 through the transverse support arm fixing through hole 1123; the second bearing bracket 130 is provided with a second supporting through hole 133, and the center position of the transverse supporting arm fixing through hole 1123 and the center position of the second supporting through hole 133 are relatively fixed. This is one of measures to ensure the coaxiality of the respective optical components on the optical axis.

In an embodiment of an imaging module carrying base for a microscopic imaging acquisition apparatus, as shown in fig. 12 to 16, the imaging target holder module 300 includes an XY axis moving platform module 310 and a reagent card holder module 320; the reagent card holder assembly 320 is for holding an external reagent card 390; the reagent card holder assembly 320 is fixed on top of the XY axis motion stage assembly 310; the XY-axis moving stage assembly 310 is fixed on top of the second carrier support 130. The reagent card holder assembly 320 comprises a reagent card holder body 321 and a reagent card holder 322; the reagent card holding portion 322 is used for holding an externally inserted reagent card 390; the bottom surface of the reagent card holder main body 321 is parallel to the bottom surface of the reagent card holder 322. The reagent card holder main body 321 and the reagent card holding portion 322 may be integrally formed or combined; or may be separate parts. The reagent card holder main body 321 is further provided with a plurality of elastic holding support members 323, the reagent card holding portion 322 comprises two reagent card holding strips, and two elastic holding support members 323 are arranged below each reagent card holding strip; an external reagent card 390 is captured between the reagent card retaining portion 322 and the resilient retaining member 323.

As shown in fig. 16, in an embodiment of an imaging component carrying base for a microscopic imaging acquisition apparatus, an XY-axis moving stage component 310 includes a moving stage base 311, a first moving component 312, a second moving component 313, a first driving motor 315, and a second driving motor 314; the first driving motor 315 is connected to the first moving assembly 312, and drives the first moving assembly 312 to move horizontally; the second driving motor 314 and the second moving assembly 313 are connected to drive the second moving assembly 313 to move horizontally; the moving directions of the first moving assembly 312 and the second moving assembly 313 are orthogonal, and the two-dimensional movement of the XY-axis moving platform assembly 310 on the horizontal plane is completed.

In an embodiment of the imaging module carrying base for the microscopic imaging acquisition apparatus, which is not shown in the drawings, a plurality of third elastic supporting members are disposed on the second carrying bracket 130 for adjusting the levelness of the bottom surface of the XY-axis moving stage assembly 310. A plurality of fourth elastic supporting members are provided on the top surface of the XY-axis moving stage assembly 310 for levelness adjustment of the bottom surface of the reagent card holder assembly 320.

As shown in fig. 9-11, an embodiment of an imaging assembly carrying base for a microscopic imaging acquisition apparatus further includes a light source assembly 500; a light source fixing circular truncated cone 136 is arranged on the second bearing bracket 130 by taking the second supporting through hole 133 as a center; the light source assembly 500 is fixed on the light source fixing round platform 136; the emergent rays of the light source in the light source assembly 500 and the imaging lens in the imaging assembly carrying device 200 are on the same imaging optical axis.

As shown in fig. 3, 9 to 17, an embodiment of a microscopic image obtaining apparatus includes an imaging component carrier 200, an imaging component carrier base and a microscopic imaging component 600; the microimaging assembly 600 is secured to the imaging lens holder 260. The microimaging assembly 600 is secured to the imaging lens holder 260. Included in microimaging assembly 600 is objective lens 610; the objective lens 610 passes through the imaging lens holder 260, the objective lens 610 is held and fixed by the imaging lens holder 260, and one end of the objective lens 610 is positioned below the plane of the bottom 261 of the imaging lens holder; the objective lens 610 is located on the target imaging optical axis; the bottom surface of imaging lens holder lens through hole 266 is imaging lens holder bottom through hole 2661.

In an embodiment of the method for adjusting a microscopic image acquisition device, not shown in the drawings, the following steps are included: step A: acquiring the offset direction of the bottom through hole 2661 of the imaging lens holder and the target imaging optical axis, and if the offset included angle between the plane of the bottom through hole 2661 of the imaging lens holder and the target imaging optical axis is greater than a set target value, entering step B; and B: selecting a limit distance adjusting fastener 272 in a limit position adjusting hole 271 closest to the end of the objective lens 610, adjusting the depth of the limit distance adjusting fastener 272 passing through downwards, and adjusting the force of the imaging lens holder bottom 261 pressing the corresponding first elastic support 233 at the point; step C: checking again the offset included angle between the plane of the through hole 2661 at the bottom of the imaging lens holder and the target imaging optical axis, and returning to the step B if the offset included angle is larger than the set target value; until the offset included angle between the plane of the through hole 2661 at the bottom of the imaging lens holder and the target imaging optical axis is less than or equal to the set target value; step D: when the offset included angle between the plane of the imaging lens holder lens through hole 266 and the target imaging optical axis is smaller than or equal to the set target value, the imaging lens holder bottom 261 and the adjusting guide 270 are fixedly connected.

As shown in fig. 19 to 21, the imaging lens holder 260, the adjustment guide 270, and the imaging lens holder base 232 are assembled together; reference numeral F in the drawing denotes a contact surface F of the lower surface of the imaging lens holder base 261 and the first elastic support 233; reference G indicates an imaging optical axis; in an ideal state, the imaging optical axis G is perpendicular to the contact surface F, which means that the imaging optical axis is orthogonal to the imaging target area, a plane image of the target imaging area can be normally microscopically amplified, and the image is clear and has no ghost. A state in which the first elastic support 233 is pressed more is illustrated in fig. 20, and a state in which the first elastic support 233 is pressed more is illustrated in fig. 21. H1 in fig. 20 is a gap from the lower surface of the imaging lens holder bottom 261 to the upper surface of the imaging lens holder bottom 232; h2 in fig. 21 is a gap from the lower surface of the imaging lens holder bottom 261 to the upper surface of the imaging lens holder bottom 232; the gap H2 is smaller than the gap H1.

As shown in fig. 22, an included angle α exists between the main body G1 of the microimaging assembly 600 and the target imaging optical axis G0, and at this time, an included angle α also exists between the plane F1 of the imaging lens holder bottom 261 and the ideal contact surface F0. When the included angle α is zero, as shown in fig. 23, ideally, the main body of the microimaging assembly 600, i.e. the actual optical axis G1, coincides with the target imaging optical axis G0, and at this time, the plane F1 of the imaging lens holder bottom 261 also coincides with the position of the ideal contact surface F0. In fig. 23, for the sake of clarity, the actual optical axis G1 is not completely aligned with the target imaging optical axis G0, and the position of the plane F1 of the imaging lens holder bottom 261 and the ideal contact surface F0 are not aligned. The actual optical axis G1 is substantially orthogonal to the plane F1 of the imaging lens holder base 261. Of course, the target imaging optical axis G0 and the ideal contact surface F0 are also perfectly orthogonal, and the point S is the intersection thereof.

In fig. 22 and 23, R1 is the distance from the first elastic supporting member 233 to the center of the lens through hole 266 of the imaging lens holder, i.e. the distance from the first elastic supporting member 233 to the central optical axis; r2 is the distance from the front end of the objective lens 610 to the imaging lens holder bottom 261; the distance L1 is a distance by which the first elastic support 233 is pressed down; the distance L2 is a moving distance of the objective lens 610 on the target imaging optical axis G0 due to the adjustment of the distance L1. tan α = L1/R1; the degree of the angle alpha can be obtained, wherein alpha = arctag (L1/R1), and L2= R2-L3= R2-R2 × cos alpha can be obtained through calculation; the angle alpha is usually very small, cos alpha is very close to 1, and therefore the actual size of L2 is usually very small. If the distance L2 is small enough, for example, if L2 is less than or equal to the depth of field of the image, the image quality of the microscopic image will not be substantially affected. When the angle α is too large, if the distance L2 is greater than the depth of field of the image, the image quality of the microscopic image may be substantially affected, and the image may be ghosted or may not be focused clearly.

Adjustment of the angle α is therefore one of the key influencing factors, whereas α = arctag (L1/R1); since R1 is a fixed constant after the system structure is finalized, the adjustment accuracy of the α angle is determined by the adjustment accuracy of L1.

L1 can be adjusted by the distance of fastener descent at the time of adjustment. I.e., the size of L1, can be controlled by spacing the distance the fastener 272 travels downward. The higher the control accuracy of the down distance of the limit distance adjusting fastener 272, the more finely the angle α can be adjusted.

In the application of the application, a direct-type fastener which can directly control the downward distance of the fastener can be adopted; the descending distance of the fastener can be adjusted and controlled through the spiral fastener, and the accuracy scale of the descending distance adjustment is improved by one level.

In the present application, distance adjustment fastener 272 is a screw-type fastener, including a set screw. The spiral fastener controls the descending distance of the spiral fastener through the rotation angle; the lead S is the descending distance of the screw fastener when the screw rotates for one circle; l1= S/360; the lead S ranges from 0.2mm to 1mm; or a limit distance adjusting fastener 272 with a lead of 0.35mm,0.5mm, 0.7mm; when L1 is greater than or equal to S, it will rotate at least one full turn, where L1= S × n + S/360, where n is the full turn of rotation. The adjustment of the descending distance of the distance adjusting fastener 272 is realized by converting the fine adjustment of the descending distance into the rotation angle and the number of turns of the distance adjusting fastener 272 through the lead S, which is equivalent to converting the fine adjustment of the distance into the adjustment of the rotation angle, enlarging the means of finely adjusting the size, and realizing the fine adjustment of the descending distance L1 by finely adjusting the rotation angle of the spacing distance adjusting fastener 272.

For example, when the lead S =0.5mm of the distance adjusting fastener 272, L1= S/360=0.5mm/360 ≈ 1.39um, which means that the downstream distance is about 1.39um every time the distance adjusting fastener 272 rotates one degree, the adjustment of the downstream distance of the order of um can be realized. Even if the rotation angle is based on 30 degrees, the adjustment precision can be 30 multiplied by 0.5mm/360 ≈ 41.7um, and is more precise compared with the dimension of mm class.

In the present application, a screw-type fastener is applied, so that the distance adjusting fastener 272 can improve the precision of distance adjustment, and the adjustment of the distance L1 in α = arctag (L1/R1) is finer, so that the adjustment of the α angle is finer, and the adjustment of the distance L2 in L2= R2-R2 × cos α is finer. In the balance between the machining accuracy and cost of the component, the dimensional adjustment with higher precision can be realized with relatively lower machining accuracy, and the method is an efficient and low-cost solution, and can realize the position adjustment with high precision with the machining size of the component with low precision. That is, the distance adjustment in the mm size between the imaging lens holder bottom 261 and the adjustment guide 270 can be converted into distance adjustment in a finer size of the objective lens 610 in the optical axis direction.

The imaging component bearing device, the base station, the microscopic image acquisition device and the adjusting method comprise an imaging lens bracket, an imaging lens clamping seat and an adjusting guide seat; the bottom of the imaging lens clamping seat is movably clamped between the imaging lens bracket and the adjusting guide seat; the levelness of the upper surface of the bottom of the imaging lens clamping seat relative to the bottom of the imaging lens support can be adjusted until a microscopic imaging assembly fixed on the imaging lens clamping seat is orthogonal to the upper surface of the bottom of the imaging lens clamping seat, and the microscopic imaging assembly is fixed on the imaging lens clamping seat. The distance adjustment in the mm size between the bottom of the imaging lens clamping seat and the adjusting guide seat is converted into the distance adjustment in the finer size of the objective lens in the optical axis direction, so that the high-precision optical adjustment process is realized.

The above descriptions of the embodiments shown in fig. 1 to 23 are only examples, and are not intended to limit the scope of the present disclosure, and all equivalent structures or equivalent processes that are modified by the contents of the specification and the drawings, or directly or indirectly applied to other related technical fields are also included in the scope of the present disclosure.

Claims (20)

1. An imaging assembly carrier (200),

the bearing is used for the imaging assembly in the microscopic imaging acquisition device;

comprises an imaging lens bracket (230), an imaging lens clamping seat (260) and an adjusting guide seat (270);

the adjusting guide seat (270) is fixedly connected with the imaging lens bracket (230);

the imaging lens holder (260) comprises an imaging lens holder bottom (261) and a lens holder (262); the imaging lens holder lens through hole (266) passes through the imaging lens holder bottom (261) and the lens holder (262); a bracket lens through hole (231) is formed in the imaging lens bracket (230);

a guide seat through hole (279) is arranged on the adjusting guide seat (270);

the lens clamping part (262) passes through a guide seat through hole (279) on the adjusting guide seat (270), so that the bottom part (261) of the imaging lens clamping seat is movably clamped between the imaging lens bracket (230) and the adjusting guide seat (270);

when imaging lens holder bottom (261) joint was adjusted between the guide holder at imaging lens support, support lens through-hole (231) and imaging lens holder lens through-hole (266)'s position corresponds.

2. Imaging assembly carrying device (200) according to claim 1,

the imaging lens holder (230) comprises an imaging lens holder bottom (232);

the holder lens through hole (231) is arranged on the imaging lens holder bottom (232);

at least three first elastic supporting pieces (233) are arranged on the bottom (232) of the imaging lens bracket, and the first elastic supporting pieces (233) are uniformly distributed by taking the bracket lens through hole (231) as the center; the elastic support surface or point of the first elastic support (233) faces upward for supporting the imaging lens holder bottom (261).

3. Imaging assembly carrying device (200) according to claim 2,

at least three limiting position adjusting holes (271) are formed in the adjusting guide seat (270), and limiting distance adjusting fasteners (272) are arranged in the limiting position adjusting holes (271);

the position of each limit position adjusting hole (271) corresponds to the position of each first elastic supporting piece (233).

4. Imaging assembly carrying device (200) according to claim 3,

the limiting position adjusting hole (271) is a limiting position adjusting through hole;

the limiting distance adjusting fastener (272) passes through the limiting position adjusting through hole to be in contact with the bottom (261) of the imaging lens clamping seat.

5. Imaging assembly carrying device (200) according to claim 3 or 4,

the limiting distance adjusting fastener (272) is a spiral fastener, and the spiral fastener controls the downward distance of the spiral fastener through the rotation angle; the lead S is the descending distance of the screw fastener when the screw rotates for one circle; the lead S of the screw-type fastener ranges from 0.2mm to 1mm.

6. Imaging assembly carrying device according to claim 3 or 4,

two position fixing through holes A1 (275) are symmetrically arranged on two sides of each limit position adjusting hole (271) on the adjusting guide seat (270);

correspondingly, two position fixing through holes B1 (265) are also arranged on the bottom (261) of the imaging lens clamping seat;

each position fixing fastener sequentially passes through the corresponding fixing through hole A1 (275) and the corresponding fixing through hole B1 (265), and the relative position between the guide seat (270) and the imaging lens clamping seat bottom (261) is fixedly adjusted.

7. The imaging assembly carrier according to claim 2,

when the imaging lens holder bottom (261) is pressed on the first elastic supporting piece (233), the descending distance range of the first elastic supporting piece (233) is 0.3mm to 2mm.

8. The imaging assembly carrier according to claim 2,

at least three second elastic supporting pieces (276) are arranged on the adjusting guide seat (270) and used for radially supporting the bottom (261) of the imaging lens clamping seat; the second elastic supporting pieces (276) are uniformly distributed and arranged by taking the geometric center of the adjusting guide seat (270) as the center, and the second elastic supporting pieces (276) are radially arranged by taking the lens through hole (266) of the imaging lens clamping seat as the center; the elastic support surface or point of the second elastic support (276) is directed toward the radial center of the imaging lens holder lens through hole (266) for contact with the sidewall of the imaging lens holder bottom (261).

9. Imaging assembly carrying device (200) according to claim 1,

the device also comprises a motor bracket (210) and a movable plate (220);

the motor bracket (210) is used for fixing a motor for driving the imaging assembly to move;

the movable plate (220) is used for being connected with a driving force output device of a motor;

the imaging lens support (230) is fixedly connected with the movable plate (220), and the imaging lens support (230) obtains moving power by means of the movable plate (220).

10. Imaging assembly carrying device (200) according to claim 9,

further comprises a motor main body (280) and a driving force output device (290);

the motor main body (280) is fixed on the motor bracket (210); the rotating shaft of the motor main body (280) is connected with a driving force output device (290); the driving force output device (290) is connected with the movable plate (220) to drive the movable plate (220) to move.

11. Imaging assembly carrying device (200) according to claim 2,

the imaging lens support (230) further comprises an imaging lens support elevation (235) and two imaging lens support half-sides elevations (236); the imaging lens support (230) is fixedly connected with the movable plate (220) through an imaging lens support vertical surface (235), so that the imaging lens support (230) can move along with the movable plate (220); the imaging lens support bottom (232) and the imaging lens support vertical face (235) are fixedly connected through two imaging lens support supporting half side vertical faces (236).

12. An imaging component bearing base station used for bearing a microscopic imaging acquisition device is characterized in that,

comprising an imaging assembly carrier (200) according to any of claims 1 to 10;

the imaging device also comprises a bearing substrate (120), a first bearing bracket (110), a second bearing bracket (130) and an imaging target clamping assembly (300);

the first bearing support (110) and the second bearing support (130) are respectively arranged on the bearing substrate (120);

the first bearing bracket (110) is used for bearing the imaging assembly bearing device (200);

the second bearing bracket (130) is used for bearing the imaging target clamping component (300), so that the imaging target clamping component (300) is arranged below the imaging component bearing device (200);

the first bearing bracket (110) comprises a longitudinal supporting column (111) and a transverse supporting arm (112), one end of the longitudinal supporting column (111) and one end of the transverse supporting arm (112) are fixedly connected,

a transverse supporting arm fixing through hole (1123) is formed in the outward extending part (1122) of the transverse supporting arm, and the imaging assembly bearing device (200) is fixed on the transverse supporting arm (112) through the transverse supporting arm fixing through hole (1123);

the second bearing bracket (130) is provided with a second supporting through hole (133), and the central position of the transverse supporting arm fixing through hole (1123) and the central position of the second supporting through hole (133) are relatively fixed.

13. The imaging assembly carrying base station according to claim 12,

the outward extending part (1122) of the transverse supporting arm is connected with the longitudinal supporting column (111) in a reinforcing mode through a reinforcing corner connector (113).

14. The imaging assembly carrying base platform of claim 12,

the bottom of the bearing substrate (120) is provided with a plurality of shockproof damping support legs (125).

15. The imaging assembly carrying base platform of claim 12,

the imaging target clamping assembly (300) comprises an XY axis moving platform assembly (310) and a reagent card clamping seat assembly (320);

the reagent card holder assembly (320) is fixed on the top of the XY axis moving platform assembly (310);

the XY-axis moving platform assembly (310) is fixed on the top of the second bearing support (130).

16. The imaging assembly carrying base station of claim 15,

the reagent card holder assembly (320) comprises a reagent card holder main body (321) and a reagent card holder (322);

a reagent card holding section (322) for holding a reagent card inserted from the outside;

the bottom surface of the reagent card holder main body (321) and the bottom surface of the reagent card holder (322) are parallel.

17. The imaging assembly carrying base station of claim 15,

the second bearing support (130) is provided with a plurality of third elastic supporting pieces for adjusting the levelness of the bottom surface of the XY-axis moving platform component (310).

18. The imaging assembly carrying base platform of claim 15,

a plurality of fourth elastic supporting pieces are arranged on the top surface of the XY-axis moving platform component (310) and used for adjusting the levelness of the bottom surface of the reagent card holder component (320).

19. The imaging assembly carrying base station according to claim 12,

further comprising a light source assembly (500);

a light source fixing round table (136) is arranged on the second bearing bracket (130) by taking the second supporting through hole (133) as the center; the light source component (500) is fixed on the light source fixing round table (136); the emergent rays of the light source in the light source component (500) and the imaging lens in the imaging component bearing device (200) are on the same imaging optical axis.

20. A microscopic image acquisition device is characterized in that,

comprising the imaging assembly carrier (200) according to any of claims 1 to 11,

or comprising the imaging assembly carrying base station of any one of claims 12 to 19;

the microscope imaging assembly (600) is further included, and the microscope imaging assembly (600) is fixed on the imaging lens clamping seat (260).

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