CN218213039U - Bottom plate and immunochromatography multi-joint detection card - Google Patents

Abstract

The utility model relates to a bottom plate and many antithetical couplet of immunity chromatography detect card. The bottom plate is provided with a liquid guide part, the liquid guide part is a multi-stage step structure with the height gradually increased, the multi-stage step structure is provided with a base surface, the base surface is used for receiving sample solution, the highest stage step of the multi-stage step structure is provided with a plurality of spaced mounting positions for mounting detection pieces, and the edge of the lowest stage step connected with the base surface is provided with a notch with a buffering effect. The immunochromatography multi-joint detection card comprising the bottom plate can realize that two or more detections can be completed by one-time sample adding, and the assembly efficiency and the time consistency of detection results can be improved.

Description

Bottom plate and immunochromatography multi-joint detection card

Technical Field

The utility model relates to an immunoassay technical field especially relates to a bottom plate and immunity chromatography allies oneself with detection card more.

Background

The transmission of the new coronaviruses is mainly a short-range respiratory droplet, and other routes (e.g., contact, fecal ostia (digestive tract), etc.) are possible.

Currently, the medical institution can confirm whether the new coronavirus is infected by nucleic acid detection, but the nucleic acid detection has at least the following limitations: (1) These detection processes are long in time and complex in operation, and generally require more than 2 to 3 hours to produce results. (2) Nucleic acid detection requires certified laboratories, expensive equipment, and trained technicians to operate.

Among the symptoms of the new coronavirus infection, the proportion of fever is not very high. In particular, as the proportion of asymptomatic infected persons increases, the fever rate is lower, and there are many symptoms similar to those of influenza, common cold and other respiratory diseases. In order to distinguish the people with the novel coronavirus infection and the flu as soon as possible, the immune colloidal gold technology is generally recommended to carry out the fast screening of the novel coronavirus and the flu. At present, a plurality of multiplex detection cards exist in the market, but one type of multiplex detection card needs each sample adding hole to add samples independently, the detection workload is large, the detection efficiency is low, and the other type of multiplex detection card can finish two or more tests by adding samples once, but the assembly efficiency is low and the time consistency of detection results is poor.

SUMMERY OF THE UTILITY MODEL

Based on this, the utility model provides a bottom plate, the immunochromatography allies oneself with detects the card including the immunity chromatography of this bottom plate more can realize that the application of sample accomplishes two and above detections and can improve the time uniformity of packaging efficiency and play testing result.

A bottom plate is provided with a liquid guide part, the liquid guide part is of a multi-stage step structure with the height gradually increased step by step, the multi-stage step structure is provided with a base surface, the base surface is used for bearing sample solution, a plurality of spaced mounting positions used for mounting a detection piece are arranged on the highest stage step of the multi-stage step structure, and a notch with a buffering effect is arranged on the edge of the lowest stage step connected with the base surface.

The liquid guide part of the bottom plate is of a multi-stage step structure, the edge of the lowest stage step connected with the base surface is provided with the notch with a buffering effect, so that the sample solution can be decelerated when entering the liquid guide part, and meanwhile, due to the interception and buffering of the multi-stage step structure, the sample solution can reach a detection piece in basically the same time, so that the time consistency of a detection result is improved, and the detection accuracy is improved; in addition, for the traditional multiple detection card that adopts the capillary to link to each other with application of sample groove and each detection piece, the packaging efficiency of the multiple detection card including above-mentioned bottom plate is higher, and the time uniformity that each test paper goes out the testing result is better.

In one embodiment, the notch is in an arc surface shape which is gradually sunken towards the side surface of the highest step.

In one embodiment, the multi-step structure is a two-step structure.

In one embodiment, the bottom plate is provided with a strip blocking portion, the liquid guide portion and the strip blocking portion cooperate to enclose a sample adding groove, and the depth of the sample adding groove is not less than the distance from the top surface of the highest step to the base surface.

In one embodiment, the ends of a plurality of the installation positions are arranged in a flush mode; and/or a plurality of the installation positions are arranged in parallel.

The immunochromatography multi-link detection card comprises a cover plate, a plurality of immunochromatography detection pieces and the bottom plate, wherein the cover plate and the bottom plate are matched to enclose a detection cavity, a liquid guide part is positioned in the detection cavity, the cover plate is provided with a sample adding hole corresponding to the basal surface, the immunochromatography detection pieces are installed on an installation position, and a gap is close to the liquid outlet end of the sample adding hole.

In one embodiment, the distance between the inner surface of the cover plate and the liquid guide part satisfies the following conditions: when the sample solution flows to the space between the top surface of the highest step of the liquid guide part and the cover plate, the sample solution can flow along the surface of the liquid guide part to the sample loading area of the immunochromatography detection piece under the pushing of capillary force.

In one embodiment, the distance from the basal surface to the inner surface of the cover plate is 0.8 mm-1.2 mm;

and/or the distance from the top surface of the lowest step to the inner surface of the cover plate is 0.6-1 mm;

and/or the distance from the top surface of the highest step to the inner surface of the cover plate is 0.2-0.5 mm.

In one embodiment, each mounting position is in a long-strip groove shape, each immunochromatography detection piece is in a long-strip shape, the immunochromatography detection pieces are embedded in the mounting positions, and the end parts, close to the top surface of the highest step, of the plurality of immunochromatography detection pieces are flush.

In one embodiment, the immunochromatographic detection piece comprises a substrate, and a sample pad, a combination pad, a detection membrane and an absorption pad which are arranged on the substrate and are sequentially connected from one end to the other end of the substrate, wherein the sample pad is provided with a sample loading area, the detection membrane is provided with a detection line, and the cover plate is provided with a detection window for observing the detection line of each immunochromatographic detection piece.

In one embodiment, the cover plate is provided with a label window at a region corresponding to the immunochromatographic detection member.

In one embodiment, the immunochromatographic test piece comprises a novel coronavirus immunochromatographic test strip and an influenza virus immunochromatographic test strip.

Drawings

FIG. 1 is a top view of an immunochromatographic multi-gang detection card of an embodiment;

FIG. 2 is a top view of the bottom plate of the immunochromatographic multi-gang detection card shown in FIG. 1;

FIG. 3 is a cross-sectional view of the immunochromatographic multi-gang test card shown in FIG. 1;

FIG. 4 is a perspective view of the bottom plate of the immunochromatographic multi-gang detection card shown in FIG. 1;

fig. 5 is a partially enlarged view of the base plate of fig. 4.

Reference numerals are as follows:

10. an immunochromatographic multiplex detection card; 110. a base plate; 120. an immunochromatographic detection member; 130. a cover plate; 111. A liquid guide part; 112. a highest-level step; 113. a ground plane; 114. a lowest step; 114a, a gap; 131. A sample application hole; 115. a bar blocking part; 112a: the top surface of the highest step; 121. a sample pad; 122. a detection membrane; 123. detecting lines; 124. a quality control line; 132. detecting a window; 133. and (4) a label window.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. Some embodiments of the invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may be present. When the terms "vertical," "horizontal," "left," "right," "upper," "lower," "inner," "outer," "bottom," and the like are used to indicate an orientation or positional relationship, it is for convenience of description only based on the orientation or positional relationship shown in the drawings, and it is not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. The term "and/or" includes any and all combinations of one or more of the associated listed items.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.

Referring to fig. 1 to 3, an embodiment of the present application provides an immunochromatographic multi-gang test card 10, in which the immunochromatographic multi-gang test card 10 includes a bottom plate 110, a plurality of immunochromatographic test pieces 120, and a cover plate 130, and the cover plate 130 and the bottom plate 110 cooperate to define a test cavity; the bottom plate 110 is provided with a liquid guide part 111, the liquid guide part 111 is positioned in the detection cavity, the liquid guide part 111 is of a multi-step structure with gradually increasing height, the highest step 112 of the multi-step structure is provided with a plurality of spaced mounting positions, the immunochromatography detection piece 120 is mounted on the mounting positions, the multi-step structure is provided with a base surface 113, the base surface 113 is used for bearing a sample solution, and the edge of the lowest step 114 connected with the base surface 113 is provided with a notch 114a with a buffering effect; the cover plate 130 has a sample application hole 131 corresponding to the base surface 113, and the notch 114a is near the liquid outlet end of the sample application hole 131.

Although there is a product that can accomplish two or more tests by one-time sample addition in the traditional immunochromatography multi-link detection card, this kind of product generally adopts the capillary as the honeycomb duct, and communicates the sample addition slot with the detection piece (for example, the test paper), but because the capillary is small in size, the fixing is difficult and can't be directly integrated on the bottom plate and the assembly efficiency is low, and because there is the difference in the length of every honeycomb duct, thereby make the sample application time of each detection piece have the difference, and then the difference in the time that each detection piece goes out the testing result is more obvious, influence the detection accuracy. The immunochromatographic multi-joint detection card 10 improves the structure of the bottom plate 110, the liquid guide part 111 is set to be of a multi-stage step structure, the edge of the lowest stage step 114 connected with the base surface 113 is provided with the notch 114a with a buffering effect, so that the speed of a sample solution entering the liquid guide part 111 can be reduced, and meanwhile, due to the interception and buffering of the multi-stage step structure, the sample solution can reach a detection part at basically the same time, so that the time consistency of a detection result is improved, the detection accuracy is improved, the immunochromatographic multi-joint detection card 10 avoids using a capillary tube as a flow guide structure, the multi-stage step flow guide structure can be directly integrated on the bottom plate 110, and the assembly efficiency is high.

Specifically, the loading hole 131 of the cover 130 is located at one end of the liquid guide 111 away from the immunochromatographic detection member 120. Further, in some embodiments, the inner diameter of the sample application hole 131 decreases from the liquid inlet end to the liquid outlet end. So set up application of sample hole 131 and be convenient for the application of sample, it is excessive to reduce.

Specifically, the liquid guide portion 111 on the bottom plate 110 serves to guide the sample solution (e.g., serum, blood, saliva, etc.) added from the loading hole 131 to the immunochromatographic detection member 120 to complete the detection. More specifically, the bottom plate 110 further has a strip blocking portion 115, the liquid guiding portion 111 and the strip blocking portion 115 cooperate to define a sample loading slot for receiving the sample solution, and the depth of the sample loading slot is adapted to the top surface 112a of the highest step.

In the illustrated embodiment, the base surface 113 and the top surface of each step of the multi-step structure are both planar structures, and the base surface 113 is disposed parallel to the top surface of each step. During detection, the basal surface 113 and the top surfaces of all steps are horizontally arranged, so that the stability of sample loading can be ensured. In the illustrated embodiment, the base surface 113 is generally arcuate with the chord of the arc flanking the lowest step 114.

Referring to fig. 4 and 5, further, the notch 114a of the lowest step 114 is in an arc shape gradually recessed toward the end where the side surface of the highest step 112 is located, and the arc-shaped side surface is connected to the base surface 113. It will be appreciated that the size of the notch 114a can be adjusted according to the actual situation. In an alternative specific example, the notch 114a is an arc surface with a chord length of 4.5mm to 7mm and a radian of 100 to 144 degrees. In the illustrated embodiment, the notch 114a is located in the middle of the lowest step 114. In the illustrated embodiment, the number of notches 114a is one. It is understood that in other embodiments, the number of the notches 114a may be more than one. For example, a plurality of notches 114a are symmetrically spaced from the middle of the lowest step 114 to both ends. Of course, the notches 114a may be arranged in a continuous manner, for example, a plurality of notches 114a may be connected in a continuous manner to form a wave shape. The plurality of notches 114a may be the same size or different sizes. For example, the notch 114a is gradually reduced in the direction from the middle of the lowest step 114 to both ends.

Further, the central axis of the notch 114a is collinear with the central axis of the loading hole 131. In an alternative specific example, the orthographic projection of the sampling hole 131 on the base surface 113 is a circle, the orthographic projection of the notch 114a on the base surface 113 is an arc outside the circle, and the center of the arc coincides with the dot of the circle.

Referring to fig. 5, further, the length of at least the highest step 112 is not less than the sum of the widths of all the immunochromatographic detection members 120 and the sum of the intervals between the immunochromatographic detection members 120. That is, at least the highest step 112 has a length not less than the distance from the edge of the left-side sample application region of the leftmost immunochromatographic test piece 120 to the edge of the right-side sample application region of the rightmost immunochromatographic test piece 120. According to the above arrangement, the sample solution can be further made to be loaded for the same time. The length of each step refers to the length of each step in the direction perpendicular to the sample flow direction, that is, the length of each step in the direction perpendicular to the length direction of the detection member in the figure. In the illustrated embodiment, the steps are of uniform length. It is understood that in other embodiments, the length of each step may not be the same.

Further, the side surface of the highest step 112 is in a symmetrical arc shape, and the side surface in the arc shape gradually protrudes from two sides to the middle to the end where the base surface 113 is located, that is, the width of the top surface of the next highest step gradually increases from the middle to two ends. After the intercepting and buffering action of the first multi-stage step structures, the sample solution reaching the top surface of the second high-stage step can flow forwards at a certain constant speed basically, when the sample solution meets the side surface of the cambered surface, the sample solution can flow towards the two ends rapidly under the guidance of the cambered surface, the side surface of the cambered surface can play a role in guiding flow, and the sample solution flowing to the two ends can continue to be converged with the sample solution flowing at the original two ends, so that the flow speed and the flow of the sample solution flowing in the middle and the two ends are further balanced.

In the illustrated embodiment, the multi-step structure is a two-step structure. That is, there is no other step between the lowest step 114 and the highest step 112 of the liquid guiding portion 111. It is to be understood that, in other embodiments, the multi-step structure is not limited to the illustrated two-step structure, and may be a step structure having more steps (e.g., three steps, four steps, etc.).

Further, a plurality of mounting positions on the base plate 110 are arranged in parallel. Further, the ends of the plurality of mounting locations on the base plate 110 are flush. The flush arrangement of the ends of the mounting positions is beneficial to the fact that the ends of the immunochromatographic detection member 120 are flush when being mounted.

Referring to fig. 4, in some embodiments, each mounting position is in the shape of a long bar, each immunochromatographic detection piece 120 is in the shape of a long bar, the immunochromatographic detection pieces 120 are embedded in the mounting positions, and the ends of the plurality of immunochromatographic detection pieces 120 close to the top surface 112a of the highest step are flush. In the illustrated embodiment, there are three immunochromatographic detection members 120, and correspondingly, there are three mounting positions. It is understood that in other embodiments, the number of immunochromatographic detection members 120 is not limited to three, but may be other numbers, such as two, four, five, and the like.

Specifically, the immunochromatographic test device 120 includes, but is not limited to, a novel coronavirus immunochromatographic test device and an influenza virus immunochromatographic test device. Optionally, the novel coronavirus immunochromatographic detection piece is an immunochromatographic test strip for detecting SARS-CoV-2 and/or a variant thereof. The immunochromatographic test strip for detecting SARS-CoV-2 and its variant strain can be one test strip, also can be several test strips. The influenza virus immunochromatography detection piece is an immunochromatography test strip for detecting influenza virus type A and/or influenza virus type B. The immunochromatographic test strip for detecting influenza virus A and influenza virus B can be one test strip or a plurality of test strips.

In an alternative specific example, the immunochromatographic detection member 120 comprises a substrate, and a sample pad 121, a binding pad, a detection membrane 122 and an absorption pad which are provided on the substrate and connected in sequence from one end to the other end of the substrate, the sample pad 121 having a loading region, the detection membrane 122 having a detection line 123. Alternatively, the substrate may be a PVC substrate. The combination pad contains fluorescent group or color reagent marked antibody. The detection membrane 122 may be a nitrocellulose membrane having a detection line 123 thereon. A control line 124 may also be disposed on the detection membrane 122.

The cover plate 130 covers the bottom plate 110. The cover plate 130 and the loading base plate 110 can be fixed together by, but not limited to, a double-sided tape or a snap structure. In the illustrated embodiment, the cover plate 130 is provided with a detection window 132 for observing the detection line 123 of each immunochromatographic detection member 120. It is understood that the detection window 132 of the cover plate 130 can be used not only for observing the detection result, but also for venting to allow the sample solution to flow smoothly in the detection chamber. It is understood that, in some examples, a vent communicating with the detection chamber may be separately provided at a region of the cover plate 130 corresponding to the immunochromatographic detection member 120. For example, a vent hole is formed at an end of the cover plate 130 far from the sample application hole 131. Of course, the detection window 132 covers at least the detection line 123 so that the detection line 123 can be observed.

In some embodiments, the distance between the inner surface of the cover plate 130 and the liquid guide portion 111 satisfies: when the sample solution flows to the space between the top surface 112a of the highest step of the liquid guide 111 and the cover plate 130, the sample solution can flow toward the sample application region of the immunochromatographic detection member 120 along the surface of the liquid guide 111 under the urging force of capillary force. Further, the distance from the base surface 113 to the inner surface of the cover plate 130 is 0.8mm to 1.2mm; and/or, the distance from the top surface of the lowest step 114 to the inner surface of the cover plate 130 is 0.6 mm-1 mm; and/or, the distance from the top surface 112a of the highest step to the inner surface of the cover plate 130 is 0.2mm to 0.5mm. Further, the distance from the base surface 113 to the inner surface of the cap plate 130 is 0.8mm to 1mm; and/or, the distance from the top surface of the lowest step 114 to the inner surface of the cover plate 130 is 0.7 mm-0.9 mm; and/or, the distance from the top surface 112a of the highest step to the inner surface of the cover plate 130 is 0.3mm to 0.4mm.

Further, in some embodiments, a label window 133 is further provided on the cover plate 130 in a region corresponding to the immunochromatographic detection member 120. The label window 133 is used to view the specific type of each immunochromatographic detection member 120.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, which is convenient for specific and detailed understanding of the technical solutions of the present invention, but can not be understood as the limitation of the protection scope of the utility model. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. It should be understood that the technical solutions obtained by logical analysis, reasoning or limited experiments based on the technical solutions provided by the present invention are all within the scope of the appended claims. Therefore, the protection scope of the present invention should be subject to the content of the appended claims, and the description and the drawings can be used to explain the content of the claims.

Claims (12)

1. The bottom plate is characterized in that the bottom plate is provided with a liquid guide part, the liquid guide part is of a multi-step structure with the height gradually increasing, the multi-step structure is provided with a base surface, the base surface is used for receiving a sample solution, a plurality of spaced mounting positions for mounting detection pieces are arranged on the highest step of the multi-step structure, and a notch with a buffering effect is arranged on the edge of the lowest step connected with the base surface.

2. The bottom plate of claim 1, wherein the notch is in the shape of a curved surface which is gradually recessed toward the side surface of the highest step.

3. The bottom plate according to claim 1 or 2, wherein the multi-step structure is a two-step structure.

4. The bottom plate according to claim 1, wherein the bottom plate has a strip blocking portion, the liquid guide portion and the strip blocking portion cooperate to define a sample addition slot, and the depth of the sample addition slot is not less than the distance from the top surface of the highest step to the bottom surface.

5. A floor according to claim 1, 2 or 4, wherein a plurality of said mounting sites are arranged flush at their ends; and/or a plurality of the installation positions are arranged in parallel.

6. An immunochromatographic multi-joint test card comprising a cover plate, a plurality of immunochromatographic test pieces and the base plate of any one of claims 1 to 5, wherein the cover plate and the base plate cooperate to define a test cavity, the liquid guide part is located in the test cavity, the cover plate is provided with a sample adding hole corresponding to the base surface, the immunochromatographic test pieces are mounted on the mounting positions, and the notch is close to the liquid outlet end of the sample adding hole.

7. The immunochromatographic multi-test card according to claim 6, wherein the distance between the inner surface of the cover plate and the liquid guide part satisfies: when the sample solution flows to the space between the top surface of the highest step of the liquid guide part and the cover plate, the sample solution can flow along the surface of the liquid guide part to the sample loading area of the immunochromatographic detection piece under the pushing of capillary force.

8. The immunochromatographic multi-test card according to claim 7, wherein the distance from the basal surface to the inner surface of the cover plate is 0.8mm to 1.2mm;

and/or the distance from the top surface of the lowest step to the inner surface of the cover plate is 0.6-1 mm;

and/or the distance from the top surface of the highest step to the inner surface of the cover plate is 0.2-0.5 mm.

9. The immunochromatographic multi-joint test card according to any one of claims 6 to 8, wherein each mounting position is in the shape of an elongated groove, each immunochromatographic test piece is in the shape of an elongated bar, the immunochromatographic test pieces are embedded in the mounting positions, and the ends of the plurality of immunochromatographic test pieces near the top surface of the highest-order step are flush.

10. The immunochromatographic multi-test card of claim 9, wherein the immunochromatographic test piece comprises a base, and a sample pad, a binding pad, a test membrane and an absorption pad which are provided on the base and connected in order from one end to the other end of the base, the sample pad has a sample loading area, the test membrane has test lines, and the cover plate is provided with a test window for observing the test lines of each immunochromatographic test piece.

11. The immunochromatographic multi-test card of claim 10, wherein a label window is provided on the cover plate in a region corresponding to the immunochromatographic test piece.

12. The immunochromatographic multiplex assay card of any one of claims 6 to 8, 10 and 11, wherein the immunochromatographic assay member comprises a novel coronavirus immunochromatographic test strip and an influenza virus immunochromatographic test strip.

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