CN218202863U - Cell culture system - Google Patents
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- CN218202863U CN218202863U CN202222208024.9U CN202222208024U CN218202863U CN 218202863 U CN218202863 U CN 218202863U CN 202222208024 U CN202222208024 U CN 202222208024U CN 218202863 U CN218202863 U CN 218202863U
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Abstract
The utility model relates to a cell culture technical field discloses a cell culture system, include: a handling frame configured with a plurality of physically independent accommodating cavities; and one or more cell culture devices which can be moved out of and loaded into the accommodating cavity of the operation frame, wherein a culture bin which is used for constructing a cell culture environment and is used for placing a culture dish is arranged in the one or more cell culture devices. The operation frame is provided with a first system for supplying gas to each accommodating cavity and a second system for supplying power to each accommodating cavity; the cell culture device is provided with a maintaining system which enables the interior of the cell culture device to maintain a desired culture environment according to the internal environment data of the cell culture device, and a built-in gas source and a built-in power supply respectively provide gas and power supply; when the cell culture device is loaded into the corresponding accommodating cavity of the cell culture device, the control is switched to supply gas to the cell culture device by the first system and charge the power supply of the cell culture device by the second system.
Description
Technical Field
The utility model relates to a cell culture technical field particularly relates to a cell culture system.
Background
Cell culture is a fundamental work in the fields related to life sciences and medical care, and it is required to maintain a cell culture dish under appropriate conditions, such as a carbon dioxide gas at a temperature of 37 degrees celsius and a concentration of 5% and a high humidity environment. Current cell culture device generally adopts box structure, by many people's used jointly, arouses the bacterial infection that mix or misuse leads to easily, because incubator internal environment is physiological environment, is favorable to bacterial growth, easily leads to bacterial infection. The cell culture box is commonly used by a plurality of people in a laboratory, and due to different use habits, not strict execution on the laboratory or scientific research management and the like, the situation that the work of the whole laboratory is completely stopped due to the comprehensive pollution of the culture box is frequently reported, and even the abnormal termination of scientific research projects and plans which invest a lot of time, manpower and material resources can be possibly caused.
In addition, because the conventional box-type cell culture device has no mobility, in the process of scientific research and experiment, a living cell sample is often required to be transported between laboratories, cells in a culture dish can deviate from a physiological state within a certain time, and the deviation time can reach dozens of minutes to several hours according to the transport distance, so that the cells deviate from a normal state, negative effects are brought to scientific research or medical application, and the transport range of the living cells is limited. Even if the cell culture apparatus and the measurement imaging apparatus are separated in different rooms in the same laboratory, the cell sample may be separated from the optimal culture environment for a while before the measurement is started, which may adversely affect scientific research or medical application.
SUMMERY OF THE UTILITY MODEL
To the problems existing in the prior art, the first aspect of the present invention provides a cell culture system, comprising:
a handling frame configured with a plurality of physically independent accommodating cavities;
one or more cell culture devices which can be moved out of and loaded into the accommodating cavity of the operation frame, wherein a culture bin for constructing a cell culture environment and placing a culture dish is arranged in the one or more cell culture devices;
wherein the handling frame has a first system for supplying gas to each containment chamber and a second system for supplying power to each containment chamber;
the cell culture device is provided with a maintaining system which enables the interior of the cell culture device to maintain a desired culture environment according to the internal environment data of the cell culture device, the maintaining system is arranged to be capable of independently realizing gas content maintenance, temperature maintenance and humidity maintenance after the cell culture device is removed from the accommodating cavity of the operation frame, and the maintaining system is respectively provided with gas and power supply by a gas source and a power supply which are arranged in the cell culture device; and is
The cell culture device also has a controller configured to control switching to providing a supply of gas to the cell culture device from the first system of the handling frame when the cell culture device is loaded into its corresponding receiving chamber, and to controlling switching to charging of a power supply to the cell culture device from the second system of the handling frame.
In some embodiments, the maintenance system comprises a gas supply device controlled by the controller, the gas supply device being arranged to supply a gas, such as carbon dioxide gas, to the interior of the cell culture device in dependence on the internal environmental data.
In some embodiments, the maintenance system comprises a temperature maintenance device controlled by the controller, the temperature maintenance device being arranged to maintain the temperature inside the incubation well within a predetermined range in dependence on the internal environmental data.
In some embodiments, the maintenance system comprises a humidity maintaining means arranged to maintain the humidity inside the culture compartment within a predetermined range.
In some embodiments, the power source internal to the cell culture device comprises at least one rechargeable battery pack, in particular removably mounted inside the cell culture device, as an internal power source.
In some embodiments, the internal environmental data includes at least one of carbon dioxide content, temperature, and humidity inside the cell culture device, such as may be detected in real time based on a carbon dioxide gas sensor, a temperature sensor, and a humidity sensor.
It should be understood that all combinations of the foregoing concepts and additional concepts described in greater detail below can be considered as part of the inventive subject matter of the present disclosure unless such concepts are mutually inconsistent. In addition, all combinations of claimed subject matter are considered a part of the inventive subject matter of this disclosure.
The foregoing and other aspects, embodiments and features of the present teachings can be more fully understood from the following description taken in conjunction with the accompanying drawings. Additional aspects of the present invention, such as features and/or advantages of exemplary embodiments, will be apparent from the description which follows, or may be learned by practice of the specific embodiments in accordance with the teachings of the present invention.
Drawings
The drawings are not intended to be drawn to scale. In the drawings, each identical or nearly identical component that is illustrated in various figures may be represented by a like numeral. For purposes of clarity, not every component may be labeled in every drawing. Embodiments of various aspects of the present invention will now be described, by way of example, with reference to the accompanying drawings, in which:
FIG. 1 is a schematic view of a cell culture apparatus according to an embodiment of the present invention.
FIG. 2 is a schematic view of the internal structure of the cell culture apparatus according to the embodiment of the present invention.
FIG. 3 is a schematic diagram of the back panel structure of the cell culture apparatus according to the embodiment of the present invention.
Fig. 4 is a schematic view of the gas path structure of the cell culture apparatus according to the embodiment of the present invention.
FIG. 5 is a schematic view of a housing having a buffer mechanism of a cell culture apparatus according to an embodiment of the present invention.
Fig. 6 is a schematic diagram of a cell culture system according to an embodiment of the present invention.
FIG. 7 is a schematic view of an interface at the bottom of the insertion direction of the housing chamber of the cell culture system according to the embodiment of the present invention.
Fig. 8 is a schematic view of the structure of the gas path of the cell culture system according to the embodiment of the present invention.
FIG. 9 is a schematic diagram showing the structure of an electrical path of the cell culture system according to the embodiment of the present invention.
Detailed Description
For a better understanding of the technical content of the present invention, specific embodiments are described below in conjunction with the accompanying drawings.
In this disclosure, aspects of the present invention are described with reference to the accompanying drawings, in which a number of illustrative embodiments are shown. Embodiments of the present disclosure are not necessarily intended to include all aspects of the invention. It should be appreciated that the various concepts and embodiments described above, as well as those described in greater detail below, may be implemented in any of numerous ways, as the disclosed concepts and embodiments are not limited to any implementation. Additionally, some aspects of the present disclosure may be used alone or in any suitable combination with other aspects of the present disclosure.
Cell culture device
With reference to the cell culture apparatus 100 of the example shown in fig. 1, by adopting the design of a built-in power supply and an air supply, the environmental parameters of the culture chamber inside the cell culture apparatus, including temperature, humidity and carbon dioxide content, are automatically and continuously monitored, and compared with the preset environmental control standard, the carbon dioxide gas is automatically supplemented by the built-in air supply, and the automatic temperature rise process is controlled, so that an ideal physiological environment favorable for cell growth is maintained in the culture chamber.
The cell culture apparatus 100, in connection with the illustrated exemplary embodiment, includes a housing that provides a carrier and support for constructing a cell culture environment. Alternatively, the case includes an outer case 100A, an inner case 100B, and a buffer material 100C disposed in an interlayer space between the outer case 100A and the inner case 100B, as shown in fig. 5.
Alternatively, the outer box 100A and the inner box 100B may be made of plastic or metal, and may be made into a certain shape, especially a regular shape, such as a square shape in the illustrated embodiment, so as to be placed in a protection box during independent use and transfer, and facilitate temporary storage and protection in a combination, stacking or other manner.
As an alternative embodiment, at least one side or bottom surface of the outer housing 100A may form a structure that facilitates a plurality of cell culture devices to be stacked or arranged side-by-side for stability, including but not limited to, for example, a slide, a slot, or a snap-fit structure.
The cushioning material 100C may be, for example, a spring, a foam material, an air bag, etc., and is advantageously disposed in the interlayer between the outer casing 100A and the inner casing 100B. In the drawings, a plurality of springs are respectively provided in the interlayer between the outer case 100A and the inner case 100B, and both ends of each spring are respectively fixed to the outer case 100A and the inner case 100B, taking the spring as an example.
In another embodiment, the cushioning material 100C may also be a plate made of a foam material, and the plate is fixed in the interlayer by glue layers, so that the outer box 100A, the inner box 100B and the cushioning material 100C form a whole.
Therefore, through the design of the double-layer box body and the buffer material, the vibration influence on the cell culture device 100 in the moving process is reduced, the heat preservation effect is improved, the temperature compensation is reduced, and the cruising ability of the system is prolonged.
FIGS. 1 and 2 schematically illustrate the design of a cell culture device.
The cell culture apparatus 100 according to the embodiment of the present invention has a maintenance system for maintaining a desired culture environment inside the cell culture apparatus 100 according to the internal environment data of the cell culture apparatus 100.
The maintenance system is configured to independently maintain gas content, temperature, and humidity within the cell culture apparatus 100 after the cell culture apparatus 100 is removed from the receiving cavity of the handling rack 1000, and the maintenance system is supplied with gas and power from the gas source and power source, respectively, that are built into the cell culture apparatus 100.
As shown in FIG. 2, the internal structure of the cell culture apparatus of the example is schematically illustrated, and at least one culture chamber 101 is disposed in the housing and located in an internal cavity defined by an inner housing 100B.
In an alternative embodiment, the interior cavity defined by inner housing 100B is divided by a partition 100D into an upper cavity portion and a lower cavity portion, with previously described growth chamber 101 located in the upper cavity portion.
The inside of the culture chamber 101 is constructed to be a physiological environment favorable for cell growth, such as a certain temperature and humidity environment and a gas environment, where a typical environment is, for example, 37 degrees celsius, 5% carbon dioxide gas, and more than 95% relative saturation humidity. In some embodiments, the relative saturation humidity may be up to 100%.
Inside the culture container 101, a culture dish 102 for cell culture is stored, as shown in FIG. 2.
The internal environmental data includes at least one of carbon dioxide content, temperature and humidity inside the culture chamber 101 of the cell culture apparatus 100, and can be detected in real time based on a carbon dioxide gas sensor, a temperature sensor and a humidity sensor.
For example, in the example shown in fig. 2, a sensor assembly for monitoring the environmental data inside the culture chamber 101 is provided inside the cell culture apparatus 100, and includes a gas sensor 109A for monitoring the carbon dioxide content, a temperature sensor 109B for monitoring the temperature inside the culture chamber, and a humidity sensor 109C for monitoring the humidity inside the culture chamber, so as to monitor and feed back the environmental parameters inside the culture chamber 101 at a set period.
In an alternative embodiment, the maintenance system comprises a gas supply device controlled by the controller 106, the gas supply device being arranged to supply carbon dioxide gas to the interior of the cell culture device 100 in dependence on the internal environmental data.
Referring to fig. 2, the gas supply means comprises at least one gas cylinder 103 disposed inside the cell culture apparatus 100, the at least one gas cylinder 103 being configured to controllably supply gas to the culture container 101 via a gas line 104. Carbon dioxide gas is stored in the gas cylinder 103
Optionally, at least one gas cylinder 103 is removably mounted inside the cell culture device 100.
In an embodiment of the present invention, the gas supply device includes a control valve at the position of the pipeline line of the gas pipeline 104 and/or the outlet of the gas bottle 103, and the controller 106 operates the control valve to open and close when the cell culture device 100 is removed from the operation frame 1000.
As shown in FIG. 2, in an alternative embodiment, a gas cylinder 103 for storing carbon dioxide gas is provided in the cell culture apparatus 100, a flow valve 103A is provided at the outlet of the gas cylinder, and the outlet of the flow valve 103A is connected to the inside of the culture container 101 via a gas pipe 104, so that carbon dioxide gas can be supplied to the culture container via the gas cylinder 103. Optionally, a pressure relief valve is provided in the conduit line of the gas conduit 104.
Wherein the first end of the gas conduit 104 may be provided with a quick change coupling, such as a non-return quick coupling, for quick connection with the outlet end of the flow valve 103A. The second end of gas pipe 104 is connected to and fitted into the side wall of culture container 101, and communicates with the inside of culture container 101.
In an alternative example, the gas cylinder 103 is of a replaceable design, and when it is desired to replace the gas cylinder, it can be replaced quickly by means of a quick-change coupling with the first end of the gas conduit 104. For example, after the gas cylinder 103 is loaded, the gas cylinder 103 is connected to the gas pipe 104 by opening the flow valve 103A.
In an alternative embodiment, the flow valve 103A may be an electrically driven flow valve, controlled by the controller 106 as previously described.
In the example of the present invention, the quick change coupler of the first end of the gas pipeline 104 and the outlet end of the flow valve 103A are designed as male and female plug-in quick change couplers.
In an alternative embodiment, the aforementioned maintenance system comprises a temperature maintenance device controlled by controller 106, which is arranged to maintain the temperature inside culture compartment 101 within a predetermined range in dependence on the internal environmental data.
Wherein the temperature maintaining means comprises at least one electric heater 108 arranged inside the cell culture apparatus 100 for heating the culture compartment.
For example, electric heater 108 is provided in the form of a heating sheet installed inside culture compartment 101, for example, at the bottom of the inside of culture compartment 101, and can be uniformly heated by the air flow inside the culture compartment.
In an alternative embodiment, the maintenance system comprises a humidity maintenance device configured to maintain the humidity inside culture compartment 101 within a predetermined range, for example at a humidity level of 100%.
Wherein the humidity maintaining means includes a humidifying device 111 provided inside the culture chamber 101.
For example, as shown in FIG. 2, the humidifying device 111 disposed inside the cultivation container 101 can be an open container with water placed horizontally, and can humidify by natural evaporation to maintain the humidity level inside the cultivation container 101 at 100%.
In an alternative embodiment, when the humidity sensor 109C detects a decrease in humidity, indicating that the humidity level in the humidifying device 111 is in a water shortage state and cannot be maintained by evaporation, water needs to be supplemented. The controller 106 can control the alarm reminding through the alarm device.
As shown in the example of fig. 2, a fan assembly 107 is further provided inside the culture compartment 101, the operation of which is controlled by the controller 106, thereby promoting the gas, temperature and humidity in the culture compartment 101 to be maintained in a uniform state by the air flow.
As shown in FIG. 2, at least one gas outlet 110 is provided on the side wall of the cultivation container 101 for equalizing the pressure inside and outside the cultivation container 101.
It should be understood that in the example shown in fig. 2, the sensor assemblies configured on cell culture device 100 for monitoring environmental data inside culture chamber 101 are all located inside culture chamber 101, and direct detection of carbon dioxide gas content, temperature and humidity is achieved. Electric heater 108 and fan assembly 107 for circulating gas in the culture chamber are disposed inside culture chamber 101.
In another embodiment, in order to keep the culture chamber 101 relatively independent and easy to clean, a second chamber body is formed inside the inner box 100B of the cell culture apparatus 100, and forms an independent space with respect to the culture chamber 101, such as a cavity of suitable shape and size located at the outer side of the culture chamber 101, facilitating the installation of the aforementioned electric heater 108, fan assembly 107 for realizing the circulation of gas inside the culture chamber, and sensor assembly therein, and is independent of the culture chamber 101 and maintains a communication state with the culture chamber 101. For example, the cavity of the second cabin body is designed into a strip shape, the two ends of the cavity are respectively communicated with the two ends of the cultivation cabin, the fan assembly 107 is arranged in the second cabin body, and the circulation of gas in the second cabin body and the cultivation cabin 101 is realized through the operation of the fan assembly 107. When the electric heater 108 is operated to heat, the temperature is uniformly heated by the circulation of hot air in the second cabin body and the culture cabin 101.
In an alternative embodiment, the fan assembly 107, the electric heater 108, and the gas sensor 109A may be disposed within the second cartridge.
As shown in connection with FIG. 2, the power source internal to cell culture apparatus 100 includes at least one rechargeable battery pack 105. The battery pack 105 supplies electric power to various consumer devices through a power supply circuit not shown in the drawings, for example, supplies electric power to the controller 106, the fan assembly 107, the electric heater 108, the gas sensor 109A, the temperature sensor 109B, the humidity sensor 109C, the humidifying device 111, and the like.
The power supply circuit can adopt the existing power supply circuit design, such as a circuit comprising one or more DC-DC modules, to realize the output of different voltages, thereby realizing the power supply to different devices.
In the foregoing embodiment, it is particularly preferable that the battery pack 106 is formed by connecting a plurality of secondary chargeable battery cells (cells) in series and/or in parallel, wherein a battery management unit (BMS) is provided for realizing charge and discharge management of the battery and battery state detection. The rechargeable battery Cell (Cell) may be a lithium-based battery or a nickel metal hydride battery.
Preferably, the battery pack 105 is detachably installed inside the cell culture device 100, the battery pack 106 is designed in a replaceable manner, and when the battery pack is insufficient in electric quantity, the battery pack can be replaced quickly, so that the battery pack can be replaced conveniently when the cell culture device 100 is used alone, and long-time independent use is realized.
In other embodiments, the cell culture apparatus 100 is configured with at least two batteries 100, allowing for a redundant design of power supplies.
In an alternative embodiment, the cell culture apparatus 100 is further configured with a charging circuit, which is connected to the aforementioned controller 106, and is connected to an external power source, especially a dc power source, to charge the battery 106. For example, the battery pack is charged by a portable mobile power source.
In an alternative embodiment, the charging circuit is correspondingly configured with an external charging interface 115, and the external charging interface 115 is used for receiving an external power input to charge the battery pack 105. The external charging interface 115 may be ase:Sub>A round interface, ase:Sub>A square interface, ase:Sub>A Type-C interface, ase:Sub>A USB-ase:Sub>A interface, or the like, and receives an external power supply input. The external charging interface 115 is connected to a charging circuit provided in the incubation chamber, and is configured to receive an external power input and charge the battery pack 105. In the example shown in fig. 3, a round interface charging interface is taken as an example for description.
In connection with the example shown in FIG. 2, the controller 106 may be implemented as an embedded processor, with low power consumption, for controlling the cell culture apparatus 100.
The fan assembly 107, the electric heater 108, and the gas sensor 109A, the temperature sensor 109B, and the humidity sensor 109C of the cell culture apparatus 100 are electrically connected to the controller 106, and are controlled by the controller 106.
As an alternative embodiment, the gas cylinder 103, the flow valve 103A, the battery pack 105 and the controller 106 are all arranged in the lower cavity. In a preferred embodiment, the window can be arranged and closed by a cover plate at the corresponding box body part around the periphery of the cavity of the lower part, and particularly the cover plate is tightly covered at the window position in a buckling mode, so that the cover plate at the window position can be operated from the outside to realize the quick replacement of the battery pack 105 or the gas cylinder 103.
In an alternative embodiment, a solenoid valve 117 is disposed on a pipeline of the gas pipeline 104 extending from the outlet of the flow valve 103A to the culture chamber 101, and is connected to the controller 106, and the controller 106 controls the opening and closing and/or opening of the solenoid valve 117. The electromagnetic valve 117 may be an electromagnetic flow valve or other electrically controlled flow valve, and is used to control the supplement of carbon dioxide gas to the culture chamber.
In some embodiments, controller 106 controls the operation of the gas supply and temperature maintenance device and monitors the status of the humidity maintenance device based on the monitored temperature, humidity, and carbon dioxide content in growth chamber 101 to regulate the environment within the growth chamber to maintain a desired growth environment.
For example, as shown in fig. 2 and 4, when the monitored carbon dioxide content is lower than a predetermined level, for example, lower than 5%, the controller 106 controls the solenoid valve 117 to open, and the gas cylinder 103 supplies carbon dioxide gas to the culture chamber 101 via the gas pipeline 104 until the carbon dioxide concentration in the culture chamber reaches the predetermined level, and controls the solenoid valve 117 to close.
When the ambient temperature in the incubation well is monitored to be below a predetermined temperature, the controller 106 controls the heater chip to operate until the temperature rises to a predetermined level, such as 37 degrees Celsius, and controls the heater chip to turn off.
In the embodiment of the present invention, the fan assembly 107 is configured to be controlled by the controller 106 to maintain continuous operation, so as to promote the gas, temperature and humidity in the cultivation chamber to maintain uniform state through continuous gas circulation. In the alternative, a constant speed or other suitable control strategy may be used to control the operation of the fan assembly 107.
In an alternative embodiment, the cell culture apparatus 100 is further configured with a communication module, such as a wireless network transceiver module, for establishing a communication connection with a user device. The communication module may be a Wifi module based on IEEE protocol, a wireless communication module based on cellular network, or a bluetooth module based on bluetooth protocol, etc., and is connected to the controller 106, so as to enable the cell culture apparatus 100 to access a wireless lan or the internet through a wireless transmission protocol, so that a user may establish a communication connection with the cell culture apparatus 100 through a device (e.g., a handheld mobile communication device, a mobile tablet computer, etc.) of the user, so as to receive detection data sent by the cell culture apparatus 100, or send a control instruction to the cell culture apparatus 100, for example, send a control instruction based on the monitored temperature, humidity, and carbon dioxide content in the culture chamber, and the controller executes the control instruction to control the operation of the gas supply apparatus, the temperature keeping apparatus, and the humidity keeping apparatus.
In some embodiments, the wireless network transceiver module includes at least two of the above-described Wifi module based on IEEE protocol, wireless communication module based on cellular network, and bluetooth module based on bluetooth protocol, which are integrated on one module in an advantageous manner.
In some embodiments, the cell culture apparatus 100 is further configured with an acceleration sensor, which is connected to the controller 106. The toppling or inverted state of the cell culture device 100 is detected by the acceleration sensor, so that irreversible consequences on the cell culture in the culture dish are avoided.
In some embodiments, as shown in fig. 1, a display screen 120 is disposed on the front panel of the cell culture apparatus 100, the display screen 120 may be an LCD display screen, an LED display screen, or the like, is connected to the controller 106, and may be powered by the battery pack 105, and displays status information of the cell culture apparatus 100, including but not limited to temperature, humidity, carbon dioxide concentration, battery level, and residual gas amount of the gas cylinder, of the culture chamber, through the display screen 120.
As an alternative embodiment, the display screen 120 may be a touch display screen.
In some embodiments, at least one input device, such as a manipulation panel-type input device, may also be provided on the front panel of the cell culture apparatus 100 for providing an input interface for manipulating the cell culture apparatus 100. Particularly preferably, the touch display panel constitutes an input device.
In an alternative example, as shown in fig. 1, at least one control button 150 may be further disposed around the display screen 120, and connected to the controller 106 to serve as a control switch for controlling the lighting of the display screen 120. For example, in some embodiments, in response to an external trigger control button, the display screen 120 is illuminated and information is displayed.
In some embodiments, the lighting mode of the display screen 120 is set to delay extinguishing, e.g., in response to an externally triggered control button, the display screen 120 is lit and information is displayed, and after a predetermined delay, the display screen 120 is automatically extinguished, thereby conserving power.
In some embodiments, as shown in FIG. 1, a handle 130 is also provided on the front panel of the cell culture device 100 for user manipulation.
In an alternative embodiment, the cell culture apparatus 100 is provided with a door that can be operated to open, and in a closed state of the door, the cell culture apparatus 100 is kept in a sealed state. When the door is opened, the culture dish 102 stored in the cell culture apparatus 100 can be handled, taken, and placed.
In some embodiments, as shown in fig. 1, a user identification module 140 is further disposed on the front panel of the cell culture apparatus 100 for providing an interface or access for confirming the identity information of the user, such as a password verification module, a biological information verification module, etc., and is connected to the controller 106 for confirming the identity information of the user, thereby allowing the user to operate the cell culture apparatus 100, including but not limited to, in response to passing the identity verification, allowing the user to open the cell culture apparatus 100 to operate a culture dish located in the culture chamber 101, regulating and controlling environmental parameters in the culture chamber 101, etc.
In other embodiments, the user authentication module 140 includes a recognizable indicia, such as a two-dimensional code or bar code, that can be scanned by a user to verify the identity, and to allow operation of the cell culture apparatus 100 in response to verification, and to otherwise disable operation of the cell culture apparatus 100.
Referring to the schematic diagram of the back panel of the cell culture apparatus 100 shown in fig. 3, a second air path interface 112 and an electrical contact 113 are provided on the back panel, wherein the second air path interface 112 preferably adopts a non-return quick connector for realizing quick docking. The electrical contacts 113 are preferably POGO-PIN compliant electrical contacts.
In an alternative embodiment, the rear panel of the cell culture apparatus 100 is further provided with a power switch 114 for controlling the internal power supply of the cell culture apparatus 100 to be turned on or off when the cell culture apparatus 100 is used independently or transferred as a mobile cell culture apparatus, so that the controller, the sensors and the execution components are powered on to operate normally.
In an alternative embodiment, the back panel of the cell culture apparatus 100 is further provided with a communication programming interface 116 for establishing communication between the cell culture apparatus 100 and a control device and programming the cell culture apparatus 100 during independent use and movement of the cell culture apparatus 100.
Cell culture system
Referring to fig. 1, a cell culture system according to an embodiment of the present invention includes a handling frame 1000 and a control system 2000. The control system 2000 may be implemented by a locally deployed computer system, a server, or a server deployed in the cloud for monitoring and controlling the operation of the operation rack 1000.
In an alternative embodiment, the control system 2000 is configured to support remote access and control, for example, interact with a remote control device such as a computer system, an intelligent terminal, etc. based on a certain protocol, send field monitoring data to the remote control device, and receive an operation instruction sent by the remote control device, so as to implement operation control on the operation frame 1000.
In connection with the example of fig. 1 and 2, the handling frame 1000 is configured with a plurality of physically independent accommodating chambers, each accommodating chamber being configured to provide an accommodating space for an independent cell culture device 100. One or more cell culture devices 100 may be removed from and loaded into the receiving cavity of the handling frame 1000.
Each cell culture apparatus 100 has a maintenance system for maintaining the inside of the cell culture apparatus 100 in a desired culture environment based on the internal environmental data of the cell culture apparatus 100.
The maintenance system is configured to independently maintain gas content, temperature, and humidity within the cell culture device 100 after the cell culture device 100 is removed from the receiving cavity of the handling frame 1000, and the maintenance system is supplied with gas and power from the gas source and power source, respectively, that are built into the cell culture device 100.
The internal environmental data includes at least one of carbon dioxide content, temperature and humidity inside the culture chamber 101 of the cell culture apparatus 100, and can be detected in real time based on a carbon dioxide gas sensor, a temperature sensor and a humidity sensor.
In an embodiment of the present invention, the handling rack 1000 is configured to centrally supply air and power to the cell culture apparatus 100 loaded into the accommodating chamber. In some embodiments, the handling rack 1000 has a first system for supplying gas to each containment chamber and a second system for supplying power to each containment chamber.
For example, in each of the accommodating chambers, when one cell culture apparatus 100 is loaded therein and loaded in place, the gas circuit connection and the electric circuit connection with the loaded cell culture apparatus 100 are established in the accommodating chamber of the handling frame 1000, and the adapter for controlling the internal environment of the loaded cell culture apparatus 100 is implemented, including:
1) The gas is supplied centrally through the handling frame 1000, and the gas passage is switched to the cell culture apparatus 100 to be loaded; and
2) The power supply built in the cell culture apparatus 100 is charged by the operation rack 1000.
Thus, when the cell culture apparatus 100 is loaded into its corresponding housing chamber, switching is made to supply gas from the first system of the operation rack 1000 to the cell culture apparatus 100 and charging of the power supply of the cell culture apparatus 100 from the second system of the operation rack 1000, thereby achieving switching from internal gas supply to centralized gas supply to the operation rack 1000.
As shown in fig. 6 and 8, a centralized gas supply 3000, for example, a gas supply steel tank loaded with compressed carbon dioxide gas, is connected to the operation frame 1000 through a pressure reducing valve 3001 and a pipe.
In an alternative embodiment, the handling frame 1000 includes a component for detecting the remaining capacity of the gas source 3000.
In connection with the illustration, the first system of the gantry 1000 includes at least one gantry gas inlet 1100 for receiving an external gas and a gas channel 1200 in communication with the gantry gas inlet 1100 for applying a gas to each receiving cavity.
In combination, the gas channel 1200 includes a main conduit extending from the scaffold gas inlet 1100 and branch conduits 1201 extending from the main conduit towards each of the receiving chambers, and the end of each branch conduit 1201 is provided with a first gas conduit interface 1202 for gas connection to the cell culture apparatus 100.
The gas source 3000 for centralized gas supply is connected to the gas inlet 1100 of the operation frame via the pressure reducing valve 3001 and a pipeline, and is communicated to the main pipeline.
Wherein the first air passage interface 1202 is configured to form a pair of male-female mating interface structures with the second air passage interface 112 of the cell culture device 100 loaded into the receiving chamber.
With reference to the illustrated example, in each accommodating cavity, the second air path interface 112 disposed on the rear panel of the cell culture apparatus 100 loaded therein and the first air path interface 1202 form a pair of male-female fitting structures, wherein both the second air path interface 112 and the first air path interface 1202 adopt a non-return quick connector design, so that quick connection and disconnection can be realized, and after the connection and disconnection, both end connectors are automatically closed, no gas leakage occurs, and the anti-fouling capability is strong, and the cleaning is easy.
In combination with the illustration, the second system of the console 1000 includes at least one console electrical inlet 1300 for receiving an external power input, and an electrical channel electrically connected to the console electrical inlet 1300 and configured to apply power to each receiving cavity. The external power input is especially commercial power input such as 220VAC and 110VAC, and can be configured according to the region where the device is used.
The electrical path comprises a power management module 1301 electrically connected to the electrical inlet 1300 of the handling frame and a wire 1302 leading from the output of the power management module 1301 to each receiving chamber, the end of the wire 1302 is provided with an electrical connector 1303, the electrical connector 1303 is arranged to contact the electrical contact 113 of the cell culture apparatus 100 loaded into the receiving chamber, and electrical communication is formed between the receiving chamber and the cell culture apparatus 100 loaded into the receiving chamber.
Wherein, the electrical connector 1303 employs a spring-loaded probe (POGO PIN) configured toward the entrance direction of the accommodating chamber.
Thus, each culture device 100 forms electrical and pneumatic communication between the cell culture device 100 and the receiving chamber into which it is installed when the receiving chamber is assembled in place.
In an alternative embodiment, the handling rack 1000 is provided with a user identity authentication module providing an interface or access for confirming user identity information for authorizing operation permission of the cell culture device 100.
For example, the user authentication module is configured to set the user authentication two-dimensional code 140 on the front panel of the cell culture apparatus 100 as an interface or an entrance for confirming user identity information, and determine the operation authority of the user on the cell culture apparatus 100 from the operation rack 1000 based on the verification result, for example, if the verification is passed, the cell culture apparatus 100 is allowed to be drawn out from the operation rack 1000, otherwise, the cell culture apparatus 100 is prohibited from being drawn out from the operation rack 1000, and the operation authorization of the cell culture apparatus 100 is realized. In further embodiments, the user identity authentication module may also be configured to perform identity authentication in other ways, including but not limited to password verification, biometric verification such as fingerprints, and the like.
In an alternative embodiment, at least one guiding mechanism, such as a guide rail or a guide groove, for providing a loading/unloading guide for the cell culture device 100, is disposed in each accommodating cavity of the handling frame 1000.
In alternative embodiments, the engagement position of the cell culture device 100 with the handling frame 1000 may also be provided with a disengagement prevention mechanism, including but not limited to an electrical, electromagnetic, mechanical design based disengagement prevention structure, or a combination thereof, to prevent accidental pulling out, slipping out, or disengagement of the cell culture device 100.
Based on the electrical communication between the electrical connector 1303 and the electrical contact 113, the control system 2000 disposed in correspondence with the operation frame 1000 communicates with the cell culture apparatus 100 and controls the charging of the battery pack 105 of the cell culture apparatus 100 to replenish the electric power.
Thus, when the cell culture apparatus 100 is mounted on the operation rack 1000 and reaches a desired position, the control system 2000 disposed in correspondence with the operation rack 1000 performs control of the cell culture apparatus 100, including control based on monitoring of carbon dioxide concentration, temperature, and humidity, after the electrical circuit connection and the air circuit connection are established.
For example, based on the electrical connection between the electrical connector 1303 and the electrical contact 113, the control system 2000 controls to close the flow valve 103A disposed in correspondence with the cell culture apparatus 100, and to block the gas supply passage from the gas cylinder 103 to the culture chamber 101.
Each cell culture device 100 is configured to send back to the control system 2000 its status information, including but not limited to one or more of temperature, humidity, carbon dioxide concentration, battery level, and cylinder residual gas, in real time or at a preset cycle.
The control system 2000 controls the corresponding actuator, such as a heating plate, in the cell culture apparatus 100 to operate based on the fact that the temperature and humidity in the culture chamber 101 of a certain cell culture apparatus 100 are lower than the preset levels, so as to remind the replacement or supplement of the humidification apparatus until the temperature and humidity in the culture chamber 101 reach the preset levels.
In some embodiments of the present invention, when the open circuit state and/or the starved state of the first system, and the open circuit state of the second system occurs, the controller 106 of the cell culture apparatus 100 can control the gas and power supplied by the gas source and power source built into the cell culture apparatus 100, respectively, and shut off the gas supply path from the first system to the cell culture apparatus 100 and the power charging path from the second system to the cell culture apparatus 100.
Thus, when an accident or a lack of gas or power occurs in the operation rack, the cell culture apparatus 100 can provide gas and power supply by itself.
For example, when the cell culture device 100 is loaded into the corresponding accommodating cavity on the operation frame, due to the lack of air in the air source 3000 (for example, the amount of residual air is lower than a preset threshold, such as 5%), the open circuit state of the first system (for example, the open circuit failure of the main or branch line, which results in the failure to smoothly deliver carbon dioxide), the open circuit state of the second system (for example, the charging of the battery pack cannot be smoothly achieved due to the open circuit caused by no external AC input or line failure, etc.), the controller 106 controls to switch to the air supply from the air bottle inside the cell culture device 100 and the power supply from the battery pack inside, so as to ensure that the cell culture device 100 can still independently and normally operate when the operation frame cannot intensively supply air and power.
Therefore, on one hand, each accommodating cavity is used as an independent bin to accommodate an independent cell culture device 100, each cell culture device 100 can be drawn out from the operation frame 1000, and on the premise of passing identity verification, connection and removal can be realized at any time based on a structure of quick plug-pull combination, so that the cell culture devices 100 can be freely operated and moved, and the cell culture device is movable, and meets the moving requirements of the cell culture devices 100 in multiple scenes; on the other hand, after the cell culture apparatus 100 is removed from the operation frame 1000, the cell culture apparatus can be used as an independent culture apparatus, and gas and power supply are provided through a gas cylinder and a battery pack which are arranged inside the cell culture apparatus 100, so that each sensor and executive device can normally operate, the culture environment inside the cell culture apparatus 100 can be maintained, the ideal physiological environment required by cell growth can be maintained, the negative influence on cell growth during moving can be reduced, meanwhile, centralized gas supply and power supply can be performed when the cell culture apparatus is installed in the operation frame, unified management and operation can be realized, and extraction, analysis and storage can be performed according to the state information and the growth process of each cell culture apparatus 100, and the purpose of scientific research can be realized.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to limit the present invention. The present invention is intended to cover by those skilled in the art various modifications and adaptations of the invention without departing from the spirit and scope of the invention. Therefore, the protection scope of the present invention is subject to the claims.
Claims (27)
1. A cell culture system, comprising:
a handling frame (1000) configured with a plurality of physically independent accommodation chambers;
one or more cell culture devices (100) which can be taken out and loaded into the accommodating cavity of the operation frame (1000), wherein culture bins (101) for constructing a cell culture environment and placing culture dishes (102) are arranged in the one or more cell culture devices (100);
wherein the handling frame (1000) has a first system for supplying gas to each containment chamber and a second system for supplying power to each containment chamber;
the cell culture device (100) is provided with a maintaining system which enables the interior of the cell culture device (100) to maintain a desired culture environment according to the internal environment data of the cell culture device (100), the maintaining system is arranged to independently realize gas content maintenance, temperature maintenance and humidity maintenance in the interior of the cell culture device (100) after the cell culture device (100) is removed from the accommodating cavity of the operation frame (1000), and the maintaining system is respectively provided with gas and power supply by a gas source and a power source which are arranged in the cell culture device (100); and is
The cell culture apparatus (100) further has a controller (106), the controller (106) being configured to control switching to providing a gas supply to the cell culture apparatus (100) from a first system of the handling rack (1000) and to controlling switching to charging a power supply of the cell culture apparatus (100) from a second system of the handling rack (1000) when the cell culture apparatus (100) is loaded into its corresponding receiving cavity.
2. Cell culture system according to claim 1, wherein the maintenance system comprises a gas supply device controlled by the controller (106) and arranged to supply carbon dioxide gas to the interior of the cell culture device (100) in dependence of the internal environmental data of the culture compartment (101).
3. Cell culture system according to claim 2, wherein the gas supply means comprises at least one gas cylinder (103) placed inside the cell culture device (100), the at least one gas cylinder (103) being arranged to controllably supply gas to the culture chamber (101) via a gas conduit (104).
4. The cell culture system of claim 3, wherein the at least one gas cylinder (103) is removably mounted inside the cell culture device (100).
5. The cell culture system according to claim 3, wherein the gas supply device comprises a control valve at a pipe line position of a gas pipe (104) and/or an outlet position of a gas cylinder (103), and the controller (106) controls opening and closing of the control valve when the cell culture device (100) is removed from the handling frame (1000).
6. Cell culture system according to claim 1, wherein the maintenance system comprises a temperature maintenance device controlled by the controller (106) and arranged to maintain the temperature inside the culture compartment (101) within a predetermined range in dependence of the internal environmental data.
7. The cell culture system of claim 6, wherein the temperature maintenance device comprises at least one electric heater (108) disposed within the cell culture device (100) for heating the culture compartment.
8. Cell culture system according to claim 1, wherein the maintenance system comprises a humidity maintaining means arranged for maintaining the humidity inside the culture compartment (101) within a predetermined range.
9. The cell culture system according to claim 8, wherein the humidity maintaining means is an open vessel containing water arranged inside the culture chamber (101), and the humidity maintaining is performed by natural evaporation.
10. The cell culture system of claim 1, wherein the power source built into the cell culture device (100) comprises at least one rechargeable battery pack (105).
11. The cell culture system of claim 10, wherein the battery (105) is removably mounted inside the cell culture apparatus (100).
12. The cell culture system of claim 10, wherein the cell culture apparatus (100) is configured with an external charging interface (115), and the external charging interface (115) is configured to receive an external power input to charge the battery pack (105).
13. The cell culture system of claim 1, wherein the cell culture device (100) further comprises a display screen module for displaying status parameters of the cell culture device.
14. The cell culture system of claim 1, wherein the cell culture apparatus (100) further comprises a user setting module for setting operating parameters of the cell culture apparatus.
15. The cell culture system of claim 1, wherein the cell culture apparatus (100) further comprises a communication module for data interaction with the handling frame (1000) or an external device.
16. The cell culture system of claim 1, wherein the first system of the handling rack (1000) comprises at least one handling rack gas inlet (1100) for receiving external gas and a gas channel (1200) in communication with the handling rack gas inlet (1100) for applying gas to each receiving chamber.
17. The cell culture system of claim 16, wherein the gas channel (1200) comprises a main line extending from the scaffold gas inlet (1100) and a branch line (1201) extending from the main line towards each containment chamber, the end of each branch line (1201) being provided with a first gas circuit interface (1202) for forming a gas circuit connection with the cell culture device (100).
18. The cell culture system of claim 17, wherein the first air path interface (1202) is configured to form a pair of male-female mating interface structures with the second air path interface (112) of the cell culture device (100) loaded into the receiving chamber.
19. The cell culture system according to claim 17, further provided with a gas source (3000) for central gas supply, the gas source (3000) for central gas supply communicating with the main line through a pressure reducing valve (3001) and a pipe access handling frame gas inlet (1100).
20. The cell culture system of claim 1, wherein the second system of the scaffold (1000) comprises at least one scaffold electrical inlet (1300) for receiving an external power input, and an electrical channel electrically connected to the scaffold electrical inlet (1300) and configured to apply power to each containment chamber.
21. The cell culture system of claim 20, wherein the electrical channel comprises a power management module (1301) electrically connected to the handling frame electrical inlet (1300) and a wire (1302) leading from an output of the power management module (1301) to each receiving chamber, wherein an end of the wire (1302) is provided with an electrical connector (1303), and the electrical connector (1303) is configured to contact an electrical contact (113) of the cell culture device (100) loaded into the receiving chamber, thereby providing electrical communication between the receiving chamber and the cell culture device (100) loaded into the receiving chamber.
22. The cell culture system according to claim 21, wherein the electrical connector (1303) is a spring-loaded probe configured to face the inlet of the receiving chamber.
23. The cell culture system according to claim 1, wherein at least one guide mechanism for providing a loading/unloading guide for the cell culture device (100) is provided in each receiving cavity of the handling frame (1000).
24. The cell culture system according to claim 1, wherein a detachment prevention mechanism is provided at a coupling position of the cell culture apparatus (100) and the handling frame (1000).
25. The cell culture system of claim 24, wherein the detachment prevention mechanism comprises a mechanical, electrical, or electromagnetic detachment prevention mechanism.
26. The cell culture system of claim 1, wherein the cell culture apparatus (100) comprises an acceleration sensor coupled to the controller (106).
27. The cell culture system according to claim 1, wherein the handling frame (1000) is provided with a user identity authentication module providing an interface or access for confirming user identity information for authorizing operation permission of the cell culture device (100).
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222208024.9U CN218202863U (en) | 2022-08-22 | 2022-08-22 | Cell culture system |
| PCT/CN2023/113494 WO2024041442A1 (en) | 2022-08-22 | 2023-08-17 | Cell culture device, system, and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222208024.9U CN218202863U (en) | 2022-08-22 | 2022-08-22 | Cell culture system |
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| CN218202863U true CN218202863U (en) | 2023-01-03 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116355758A (en) * | 2023-06-01 | 2023-06-30 | 翔鹏佑康(北京)科技有限公司 | Tumor infiltration lymphocyte culture vessel |
| WO2024041442A1 (en) * | 2022-08-22 | 2024-02-29 | 国科温州研究院(温州生物材料与工程研究所) | Cell culture device, system, and method |
-
2022
- 2022-08-22 CN CN202222208024.9U patent/CN218202863U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024041442A1 (en) * | 2022-08-22 | 2024-02-29 | 国科温州研究院(温州生物材料与工程研究所) | Cell culture device, system, and method |
| CN116355758A (en) * | 2023-06-01 | 2023-06-30 | 翔鹏佑康(北京)科技有限公司 | Tumor infiltration lymphocyte culture vessel |
| CN116355758B (en) * | 2023-06-01 | 2023-09-29 | 翔鹏佑康(北京)科技有限公司 | Tumor infiltration lymphocyte culture vessel |
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