CN218131974U

CN218131974U - Nucleic acid ion purification suction head

Info

Publication number: CN218131974U
Application number: CN202221113452.7U
Authority: CN
Inventors: 耿景洋; 何芬; 史利利
Original assignee: Hangzhou Dipu Medical Laboratory Co ltd
Current assignee: Hangzhou Dipu Medical Laboratory Co ltd
Priority date: 2022-05-09
Filing date: 2022-05-09
Publication date: 2022-12-27
Anticipated expiration: 2032-05-09

Abstract

The utility model provides a nucleic acid ion purifies suction head, including suction head section, fillter section, storage section and linkage segment, the fillter section is located the suction head section and stores between the section, and the linkage segment setting is kept away from the one end of suction head section on storing the section, is equipped with suction head chamber, filter chamber and storage chamber in suction head section, fillter section, the storage section respectively, is equipped with first filter membrane between filter chamber and the suction head chamber, is equipped with the filter media in the filter chamber, and first filter membrane keeps apart the filter media outside the suction head chamber. The utility model discloses can effectively get rid of the salt ion among the nucleic acid sample, and simple structure, operating method is simple, is favorable to using widely on a large scale.

Description

Nucleic acid ion purification suction head

Technical Field

The utility model relates to a mass spectrometry detects technical field, in particular to nucleic acid ion purifies suction head.

Background

The rise of life science and the rapid development of new drug synthesis after the middle of the 80 s of the 20 th century are urgent for the corresponding mass spectrometry analysis method. The use of TOF-MS was active in the 90 s of the 20 th century. Later, the application of pulse ion generators is gradually expanded, and MALDI-TOF-MS is an important means for genomics and proteomics research in the field of biology. After decades of development, MALDI-TOF-MS has been widely used in molecular weight determination and structure analysis of biomacromolecules such as nucleic acid, sugar, protein and the like and synthetic polymer molecules due to its advantages of simplicity, rapidness, high throughput and high sensitivity, and has been widely used in analysis fields such as biology, medicine, food, environment and the like.

After the nucleic acid sample used in the MALDI-TOF-MS technology is subjected to PCR amplification reaction/extension reaction, a reaction product contains a large number of salt ions, such as metal cations such as Na +, K +, mg2+ and the like, which are referred to as salt ions for short, and the salt ions can interfere the result and are not beneficial to analysis and interpretation of the result, so that the nucleic acid sample can be subjected to mass spectrometry detection after being removed by the salt ions.

When the existing nucleic acid sample is used for removing salt ions, the related device and operation method have great complexity, and the application of the technology is limited to a certain extent.

Disclosure of Invention

The utility model aims at solving the problem that the prior nucleic acid sample has great complexity when removing salt ions and the related device and the operation method, providing a nucleic acid ion purification suction head which can effectively solve the problems.

The utility model aims at realizing through the following technical scheme: the utility model provides a nucleic acid ion purifies suction head, includes suction head section, fillter section, storage section and linkage segment, and the fillter section is located between suction head section and the storage section, and the linkage segment setting is equipped with suction head chamber, filter chamber and storage chamber respectively in keeping away from the one end of suction head section on the storage section in suction head section, fillter section, the storage section, is equipped with first filter membrane between filter chamber and the suction head chamber, is equipped with the filter media in the filter chamber, and first filter membrane keeps apart the filter media outside the suction head chamber.

The utility model discloses when using, with nucleic acid ion purification suction head and air power source device, the air power source device uses the pipette as an example, after nucleic acid ion purification suction head is connected to the pipette, absorb liquid nucleic acid sample through the pipette, the pipette produces the negative pressure and inhales nucleic acid sample in the nucleic acid ion purification suction head, nucleic acid sample is at the inspiratory in-process, nucleic acid sample loops through the suction head chamber on the suction head, the filter chamber finally reachs in the storage chamber, because nucleic acid sample is through the filtration of filter media, the filter media is got rid of the salt ion in the nucleic acid sample; then the nucleic acid sample in the storage cavity is added to the mass spectrum analyzer through the pipette, and then the nucleic acid sample in the storage cavity flows out from the storage cavity towards the direction of the suction head cavity, and in the process, the nucleic acid sample passes through the filter material in the filter cavity again, so that the secondary filtration of the nucleic acid sample is realized. The first filter membrane can prevent the filter material from entering the suction head cavity from the filter cavity. The utility model discloses simple structure, operating method is simple, is favorable to using widely on a large scale.

Preferably, the diameter of the end of the filter section connected to the storage section is larger than the diameter of the other end. The diameter of one end of the filtering section, which is connected with the storage section, is larger than that of the other end of the filtering section, so that the overall diameter of the suction head section is smaller, and the suction head section can conveniently extend into a sample for sampling; and the overall diameter of the storage section is larger, thereby increasing the capacity of the storage cavity.

Preferably, a second filter membrane is arranged between the filter cavity and the storage cavity, and the second filter membrane isolates the filter material outside the storage cavity. The filter media is placed between first filter membrane and second filter membrane to retrain the filter media, with its restriction in the filter chamber, avoid the filter media to get into in the storage chamber.

Preferably, the filter medium is a cation exchange resin, and the particle diameter of the cation exchange resin is 53um to 106um.

Preferably, the pore diameter of the filtration pores on the first filter membrane and the second filter membrane is 30um-40um.

Preferably, the material of the first filter membrane and the second filter membrane is isopropene or polycarbonate.

Preferably, the inner wall of the suction head cavity is provided with a flow guide strip, the flow guide strip protrudes out of the inner wall of the suction head cavity, and the flow guide strip is spirally arranged along the length direction of the suction head cavity. Through set up spiral helicine water conservancy diversion strip on the inner wall in suction head chamber, like this when liquid is inhaled in the suction head chamber, the water conservancy diversion strip can promote liquid to take place rotatory flow, and liquid can be better through the flow of self and filter media hybrid contact when passing through the filter chamber like this, improves the filter effect.

The utility model has the advantages that: the utility model discloses can effectively get rid of the salt ion among the nucleic acid sample, and simple structure, operating method is simple, is favorable to using widely on a large scale.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

In the figure: 1. the filter comprises a suction head section, 2, a filtering section, 3, a storage section, 4, a connecting section, 5, a filter material, 6, a first filter membrane, 7, a second filter membrane, 8 and a flow guide strip.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art all belong to the protection scope of the present invention.

It will be understood by those skilled in the art that in the present disclosure, the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships that are based on those shown in the drawings, which are merely for convenience in describing the present disclosure and to simplify the description, and are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus the terms are not to be construed as limiting the invention.

It is understood that the terms "a" and "an" should be interpreted as meaning that a number of one element or element is one in one embodiment, while a number of other elements is one in another embodiment, and the terms "a" and "an" should not be interpreted as limiting the number.

As shown in figure 1, a nucleic acid ion purification suction head comprises a suction head section 1, a filtering section 2, a storage section 3 and a connecting section 4, wherein the filtering section 2 is positioned between the suction head section 1 and the storage section 3, and the connecting section 4 is arranged at one end, far away from the suction head section 1, of the storage section 3. The suction head section 1, the filtering section 2 and the storage section 3 are respectively provided with a suction head cavity, a filtering cavity and a storage cavity, and the suction head section 1, the filtering section 2 and the storage section 3 are communicated with each other.

Preferably, the diameter of one end of the filtering section 2 connected with the storage section 3 is larger than that of the other end, so that the overall diameter of the suction head section 1 is smaller, and the suction head section can conveniently extend into a sample for sampling; whereas the overall diameter of the storage section 3 is larger, thereby increasing the capacity of the storage cavity.

The filter cavity is provided with a filter material 5. In this embodiment, the filter medium 5 is a cation exchange resin in the form of particles, and the particle diameter of the cation exchange resin is 53um to 106um.

A first filter membrane 6 is arranged between the filter cavity and the suction head cavity, and the filter material 5 is isolated outside the suction head cavity by the first filter membrane 6. Preferably, the aperture of the filter pores on the first filter membrane 6 is 30um-40um, and the material of the first filter membrane 6 is isopropene or polycarbonate. The adsorption capacity of the isopropene and the polycarbonate to the nucleic acid is low, and the adsorption capacity of the first filter membrane to the nucleic acid can be effectively reduced.

A second filter membrane 7 is arranged between the filter cavity and the storage cavity, and the filter material 5 is isolated outside the storage cavity by the second filter membrane 7. The filter material 5 is placed between the first filter membrane 6 and the second filter membrane 7 so as to constrain the filter material 5, confining it in the filter chamber, avoiding the filter material 5 entering the storage chamber. Preferably, the pore diameter of the filtration pores on the second filter membrane 7 is 30um-40um, and the material of the second filter membrane is isopropene or polycarbonate.

Furthermore, a flow guide strip 8 is arranged on the inner wall of the suction head cavity, the flow guide strip 8 protrudes out of the inner wall of the suction head cavity, and the flow guide strip 8 is spirally arranged along the length direction of the suction head cavity. Through set up spiral helicine water conservancy diversion strip on the inner wall in suction head cavity, when liquid was inhaled in the suction head cavity like this, the water conservancy diversion strip can promote liquid to take place rotatory flow, and liquid is when the filter chamber is being passed through to liquid like this, and liquid can be better through the flow of self with filter media hybrid contact, improves the filter effect.

The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by the teaching of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as the present invention, fall within the protection scope of the present invention.

Claims

1. The utility model provides a nucleic acid ion purification suction head, a serial communication port, including suction head section (1), filtering segment (2), storage section (3) and linkage segment (4), filtering segment (2) are located between suction head section (1) and storage section (3), one end of keeping away from suction head section (1) is set up in storage section (3) in linkage segment (4), suction head section (1), filtering segment (2), be equipped with the suction head chamber in storage section (3) respectively, filter chamber and storage chamber, be equipped with first filter membrane (6) between filter chamber and the suction head chamber, be equipped with filter media (5) in the filter chamber, first filter membrane (6) keep apart filter media (5) outside the suction head chamber.

2. The nucleic acid ion purification tip of claim 1, wherein the diameter of the end of the filtration section (2) connected to the storage section (3) is larger than the diameter of the other end.

3. The nucleic acid ion purification suction head according to claim 1, wherein a second filter membrane (7) is arranged between the filter chamber and the storage chamber, and the second filter membrane (7) isolates the filter medium (5) from the storage chamber.

4. The nucleic acid ion purification suction head according to claim 3, wherein the filter medium (5) is a cation exchange resin, and the particle diameter of the cation exchange resin is 53um-106um.

5. The nucleic acid ion purification tip of claim 4 wherein the filter pores of the first filter (6) and the second filter (7) have a pore size of 30um to 40um.

6. The nucleic acid ion purification tip as claimed in claim 4, wherein the material of the first filter (6) and the second filter (7) is isopropenyl or polycarbonate.

7. The nucleic acid ion purification suction head according to any one of claims 1 to 6, wherein a flow guide strip (8) is arranged on the inner wall of the suction head cavity, the flow guide strip (8) protrudes out of the inner wall of the suction head cavity, and the flow guide strip (8) is spirally arranged along the length direction of the suction head cavity.