CN218107709U

CN218107709U - Microfluidic product plate for screening tumor cells by using immunomagnetic beads

Info

Publication number: CN218107709U
Application number: CN202222572055.2U
Authority: CN
Inventors: 熊霞辉
Original assignee: Suzhou Diketong Biotechnology Co ltd
Current assignee: Suzhou Diketong Biotechnology Co ltd
Priority date: 2022-09-28
Filing date: 2022-09-28
Publication date: 2022-12-23
Anticipated expiration: 2032-09-28

Abstract

The utility model discloses a micro-fluidic product board for screening tumor cells by utilizing immunomagnetic beads, which belongs to the technical field of tumor cell screening, a product core board pipeline processing mechanism which comprises dust-free injection molding or micro-injection molding is arranged on a product core board and is used for carrying out target cell screening operation on injected liquid, the pipeline processing mechanism comprises an input pipe, a plurality of conveying pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe and an output pipe, a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe and a blood cell recovery pipe are arranged on the input pipe in a communicating way, and a sheath flow injection pipe, a waste liquid recovery pipe and a target cell recovery pipe are arranged on the output pipe in a communicating way; the sheath flow injection tube I, the immunomagnetic bead injection tube, the blood cell recovery tube, the electromagnet and the sheath flow injection tube II are matched for use, the separation of impurity cells is completed at the front end of the same product core plate, and the injection and mixing of immunomagnetic beads are completed at the middle end; the back end completes the accurate sorting of the target cells.

Description

Microfluidic product plate for screening tumor cells by using immunomagnetic beads

Technical Field

The utility model belongs to the technical field of the tumour cell screening, concretely relates to utilize micro-fluidic product board of immunomagnetic bead screening tumour cell.

Background

Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids using microchannels, and is an emerging interdiscipline that involves chemical, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Because of the miniaturization, integration, and other features, microfluidic devices are generally called microfluidic chips, also called lab-on-a-chip and micro total analysis systems, and one of the important features of microfluidics is that it has unique fluid properties in a micro-scale environment, such as laminar flow, droplet, and the like. With the aid of these unique fluidic phenomena, microfluidics can achieve a series of microfabrication and micromanipulation that are difficult to accomplish by conventional methods, and is currently considered to have great development potential and broad application prospects in biomedical research.

At present, the domestic market utilizes immunomagnetic beads to screen tumor cells in a few clinical rapid detection modes; in addition, on the same microfluidic chip, the product which completes magnetic bead injection and magnetic bead mixing and then realizes electromagnetic precise sorting is not available.

Compared with a biological electrode screening method commonly used in foreign platforms, a fluorescence accurate positioning method commonly used in laboratories and the like, the method has extremely high process requirements on the manufactured carrier. On the premise of large population base and large use base in China, two problems of timeliness and cost exist in the aspect of application of clinical examination and in-vitro diagnosis.

For example, the bioelectrode screening method requires vacuum sputtering of a plurality of metal films on a glass substrate; then, displaying the electrode pattern by a photoetching method; the process is complex, the cost is high, and mass production is not facilitated; for example, the precise fluorescent labeling method requires that a dense array of concave holes are manufactured on a glass substrate by photolithography or laser micro-engraving according to the size of target cells, and is complex in general process, high in cost and not beneficial to large-scale mass production.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an utilize micro-fluidic product board of immunomagnetic bead screening tumor cell to solve the current bioelectrode screening method that proposes in the above-mentioned background art and fluorescence accurate positioning method technology complicated, with high costs and be difficult to mass production, and can not inject into and the accurate problem of sorting out and going on in succession of electromagnetism is recycled in the magnetic bead mixture.

In order to achieve the above object, the utility model provides a following technical scheme: a microfluidic product plate for screening tumor cells by using immunomagnetic beads comprises a product core plate which is formed by dust-free injection molding or micro injection molding;

the device comprises a product core plate, a pipeline processing mechanism and a control mechanism, wherein the product core plate is provided with a plurality of conveying pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe and an output pipe;

the protective cover is fixed on the product core plate and covers the electromagnetic adsorption unit, a closed cavity is formed between the protective cover and the product core plate, the closed cavity is respectively communicated with the first connecting pipe and the second connecting pipe, and the other end of the second connecting pipe is communicated with the output pipe.

By adopting the scheme, the sheath flow injection tube I, the immunomagnetic bead injection tube, the blood cell recovery tube, the electromagnet and the sheath flow injection tube II are matched for use, the separation of impurity cells is completed at the front end of the same product core plate, and the injection and mixing of immunomagnetic beads are completed at the middle end; the back end finishes the accurate sorting of target cells, and through setting up the cooperation of immunomagnetic bead injection pipe and using, the screening operation is realized just to the cooperation electro-magnet of different target cells to same product core board as long as inject different immunomagnetic beads, utilizes traditional dustless injection moulding technology and little injection moulding technology, makes the product core board, can be fast and the off-the-shelf production encapsulation of large batch completion, and is with low costs, and technology simple structure.

In a preferred embodiment, the sample injection tube, the first sheath flow injection tube, the immunomagnetic bead injection tube, and the second sheath flow injection tube are disposed vertically upward, and the blood cell recovery tube, the target cell recovery tube, and the waste liquid recovery tube are disposed vertically downward.

By adopting the scheme, the sample injection pipe which is arranged upwards is convenient for injecting sample liquid, the sheath flow injection pipe I and the immunomagnetic bead injection pipe are convenient for injecting sheath flow and immunomagnetic beads, the blood cell recovery pipe is arranged downwards and is convenient for rapidly recovering blood cells, and the target cell recovery pipe and the waste liquid recovery pipe are convenient for recovering target cells and waste liquid.

As a preferred embodiment, the conveying pipes are arranged in a serpentine shape, and a plurality of conveying pipes arranged in a serpentine shape are communicated with one another end to end.

Adopt above-mentioned scheme, utilize the conveyer pipe that snakelike laid, and a plurality of conveyer pipes cooperation end to end connection, the abundant transport of the liquid of being convenient for mixes.

As a preferred embodiment, the electromagnetic adsorption unit includes an electromagnet, and the electromagnet is installed on the product core board and is electrically connected to an external power supply.

Adopt above-mentioned scheme, utilize the electro-magnet cooperation to use, can utilize electro-magnet adsorption effort cooperation immunomagnetic bead to use, immunomagnetic bead carries out the motion under the electro-magnet magnetic action and does directional removal to reach the purpose of separation viral factor.

In a preferred embodiment, the first sheath flow injection tube and the immunomagnetic bead injection tube are respectively located on the front side and the rear side of the blood cell recovery tube.

By adopting the scheme, the blood cell recovery tube is arranged between the sheath flow injection tube I and the immunomagnetic bead injection tube, so that the separation of impurity cells and the injection and mixing of the immunomagnetic beads at the middle ends can be completed at the front end of the same product core plate.

In a preferred embodiment, the protective cover is filled with nitric oxide gas.

Adopt above-mentioned scheme, utilize the nitric oxide gas to fill in the protection casing, can avoid outside gas to enter into the protection casing inside, played fine isolated protective effect.

Compared with the prior art, the beneficial effects of the utility model are that:

the microfluidic product board for screening tumor cells by using the immunomagnetic beads is matched with a sheath flow injection pipe I, an immunomagnetic bead injection pipe, a blood cell recovery pipe, an electromagnet and a sheath flow injection pipe II, the separation of impurity cells is completed at the front end of the same product core board, and the injection and mixing of the immunomagnetic beads are completed at the middle end; the back end completes the accurate sorting of the target cells.

The microfluidic product board for screening tumor cells by using the immunomagnetic beads is matched with an immunomagnetic bead injection tube for use, and the same product core board can be matched with an electromagnet to realize screening operation corresponding to different target cells as long as different immunomagnetic beads are injected;

the microfluidic product plate for screening tumor cells by using the immunomagnetic beads is used for manufacturing the product core plates by using the traditional dust-free injection molding process and the traditional micro injection molding process, can be used for rapidly completing the production and packaging of finished products in large batch, and has low cost and simple process structure.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic structural view of another aspect of the present invention;

fig. 3 is a schematic structural view of the protective cover of the present invention;

fig. 4 is a schematic structural diagram of the pipeline treatment mechanism of the present invention.

In the figure: 1. a product core board; 2. a pipeline processing mechanism; 3. a protective cover; 4. an input tube; 5. a sample injection tube; 6. a sheath flow injection tube I; 7. an immunomagnetic bead injection tube; 8. a blood cell recovery tube; 9. a delivery pipe; 10. a first connecting pipe; 11. an electromagnet; 12. a second connecting pipe; 13. an output pipe; 14. a sheath flow injection tube II; 15. a target cell recovery tube; 16. a waste liquid recovery pipe.

Detailed Description

Referring to fig. 1-4, the present invention provides a microfluidic product plate for screening tumor cells using immunomagnetic beads, which includes a dust-free injection molded or micro-injection molded product core plate 1.

The pipeline processing mechanism 2 is arranged on the product core plate 1 and used for carrying out target cell screening operation on injected liquid, the pipeline processing mechanism 2 comprises an input pipe 4, a plurality of conveying pipes 9, a first connecting pipe 10, an electromagnetic adsorption unit, a second connecting pipe 12 and an output pipe 13, a sample injection pipe 5, a first sheath flow injection pipe 6, an immunomagnetic bead injection pipe 7 and a second sheath flow injection pipe 14 which are vertically arranged upwards, a blood cell recovery pipe 8, a target cell recovery pipe 15 and a waste liquid recovery pipe 16 which are vertically arranged downwards, the sample injection pipe 5 which is arranged upwards is convenient for injecting sample liquid, the first sheath flow injection pipe 6 and the immunomagnetic bead injection pipe 7 are convenient for injecting sheath flow and immunomagnetic beads, the blood cell recovery pipe 8 is arranged downwards and is convenient for rapidly recovering blood cells, and the target cell recovery pipe 15 and the waste liquid recovery pipe 16 are convenient for recovering target cells and waste liquid.

The electromagnetism adsorbs the unit and includes electro-magnet 11, and electro-magnet 11 is installed on product core board 1 and electro-magnet 11 and external power electric connection, uses the cooperation of electro-magnet 11 to use, can use the cooperation immunomagnetic bead of electromagnetism adsorption effort cooperation to use, and the immunomagnetic bead carries out the motion under 11 magnetic actions of electro-magnet and is directional removal to reach the purpose of separating the viral factor.

Sheath flow injection pipe 6 and immunomagnetic bead injection pipe 7 are located the front and back both sides of blood cell recovery pipe 8 respectively, through set up blood cell recovery pipe 8 between sheath flow injection pipe 6 and immunomagnetic bead injection pipe 7, can accomplish the separation of impurity cell at the front end on same product core board 1, the injection and the mixing of the immunomagnetic bead of middle-end.

The input tube 4 is communicated with and provided with a sample injection tube 5, a sheath flow injection tube I6, an immunomagnetic bead injection tube 7 and a blood cell recovery tube 8, the output tube 13 is communicated with and provided with a sheath flow injection tube II 14, a waste liquid recovery tube 16 and a target cell recovery tube 15, the input tube 4 is communicated with the delivery tube 9 at the forefront side, and the delivery tube 9 at the rearmost side is communicated with one end of the connecting tube I10.

Conveyer pipe 9 specifically becomes snakelike laying, and the end to end intercommunication each other between the conveyer pipe 9 that a plurality of was snakelike laying, utilizes the conveyer pipe 9 that snakelike laying, and a plurality of conveyer pipes 9 cooperation end to end connection, and the abundant transport of the liquid of being convenient for mixes.

The protection casing 3 is fixed on the product core plate 1 and covers the outside of the electromagnetic adsorption unit, a closed cavity is formed between the protection casing 3 and the product core plate 1, the closed cavity is respectively communicated with the first connecting pipe 10 and the second connecting pipe 12, the other end of the second connecting pipe 12 is communicated with the output pipe 13, nitric oxide gas is filled in the protection casing 3, the external gas can be prevented from entering the protection casing 3, and a good isolation protection effect is achieved.

When the device is used, liquid to be screened is injected through the sample injection pipe 5, after injection, sheath liquid is added from the sheath flow injection pipe 6, blood cells are discharged from the blood cell recovery pipe 8, required immunomagnetic beads are added from the immunomagnetic bead injection pipe 7, the required immunomagnetic beads are conveyed to the conveying pipe 9 through the input pipe 4, then the required immunomagnetic beads are conveyed to the interior of the protective cover 3 through the conveying pipe 9 and the connecting pipe one 10, the magnetic acting force is generated by the work of the electromagnet 11, the immunomagnetic beads are driven to move, screening operation is achieved, conveying is carried again through the connecting pipes two 12 and the output pipe 13, the sheath liquid is injected again through the sheath flow injection pipe two 14, waste liquid is discharged through the waste liquid recovery pipe 16, and target cells are discharged through the target cell recovery pipe 15.

Claims

1. A microfluidic product plate for screening tumor cells by using immunomagnetic beads is characterized in that: comprises a product core plate (1) formed by dust-free injection molding or micro injection molding;

the device comprises a product core plate (1), a pipeline processing mechanism (2) and a control unit, wherein the pipeline processing mechanism (2) is arranged on the product core plate (1) and used for performing target cell screening operation on injected liquid, the pipeline processing mechanism (2) comprises an input pipe (4), a plurality of conveying pipes (9), a first connecting pipe (10), an electromagnetic adsorption unit, a second connecting pipe (12) and an output pipe (13), a sample injection pipe (5), a first sheath flow injection pipe (6), an immunomagnetic bead injection pipe (7) and a blood cell recovery pipe (8) are communicated and installed on the input pipe (4), a second sheath flow injection pipe (14), a waste liquid recovery pipe (16) and a target cell recovery pipe (15) are communicated and installed on the output pipe (13), the input pipe (4) is communicated with the conveying pipe (9) at the front side, and the conveying pipe (9) at the rear side is communicated with one end of the first connecting pipe (10);

the protection cover (3) is fixed on the product core plate (1) and covers the electromagnetic adsorption unit, a closed cavity is formed between the protection cover (3) and the product core plate (1), the closed cavity is respectively communicated with the first connecting pipe (10) and the second connecting pipe (12), and the other end of the second connecting pipe (12) is communicated with the output pipe (13).

2. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, wherein: the sample injection tube (5), the sheath flow injection tube I (6), the immunomagnetic bead injection tube (7) and the sheath flow injection tube II (14) are all vertically arranged upwards, and the blood cell recovery tube (8), the target cell recovery tube (15) and the waste liquid recovery tube (16) are all vertically arranged downwards.

3. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, wherein: the conveying pipes (9) are distributed in a snake shape, and the conveying pipes (9) distributed in the snake shape are communicated end to end.

4. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, wherein: the electromagnetic adsorption unit comprises an electromagnet (11), wherein the electromagnet (11) is installed on the product core plate (1), and the electromagnet (11) is electrically connected with an external power supply.

5. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, wherein: the sheath flow injection tube I (6) and the immunomagnetic bead injection tube (7) are respectively positioned at the front side and the rear side of the blood cell recovery tube (8).

6. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, wherein: and the protective cover (3) is filled with nitric oxide gas.