CN218003229U

CN218003229U - Optical fiber grating

Info

Publication number: CN218003229U
Application number: CN202221645723.3U
Authority: CN
Inventors: 谢成昆; 曾文彬; 何峰; 杨梅; 刘美慧
Original assignee: Hunan Chaoji Testing Technology Co ltd
Current assignee: Hunan Chaoji Testing Technology Co ltd
Priority date: 2022-06-29
Filing date: 2022-06-29
Publication date: 2022-12-09
Anticipated expiration: 2032-06-29

Abstract

The utility model discloses a fiber grating, with the terminal surface interval arrangement of many optic fibre one ends, the terminal surface of every optic fibre is as the daylighting point of gathering light signal, to examining the thing to be examined on the pick-up plate and scan the discernment, constitutes fiber grating, is used for waiting to examine thing (201) and scans the discernment. The mode of combining the grating and the optical fiber into a whole can reduce the transmission loss of optical signals, improve the detection sensitivity of the optical signals and enable the structure of a scanning recognition system to be more compact.

Description

Optical fiber grating

Technical Field

The utility model relates to a detect technical field, realize digital monomolecular scanning, concretely relates to fiber grating.

Background

Single Molecule Detection (SMD) is an ultra-sensitive Detection technique that has been rapidly developed in recent years, which means that a target object is measured and analyzed at a Single Molecule level, and is a brand new Detection method and opens up a brand new Detection field. However, the existing Simoa technology adopts the number of luminescent analytes in a picture after imaging, which has higher requirements on imaging equipment and slow detection speed, and causes difficulty in application and popularization of the Simoa technology. Therefore, there is a need for an improved single-molecule detection system, and the use of a scanning recognition system is a new single-molecule detection method.

The scanning recognition system comprises: the device comprises a moving device, a detection plate, an excitation light source, a grouped straight-line grating, an optical fiber and a recognition device. The detection board is arranged on the moving device, and the detection board is provided with the to-be-detected objects distributed in a matrix. Scanning the detection plate by utilizing the relative motion formed by the grouped straight-row grating and the detection plate; the light emitted or reflected by the object to be detected is collected by the grouped straight-line grating and is transmitted to the identification device for photoelectric conversion, so that the detection plate is scanned and identified. The scanning identification mode can dynamically and continuously count the objects to be detected on the detection plate so as to realize digital single-molecule scanning, thereby improving the detection precision, reducing the identification time and improving the detection efficiency.

In order to further simplify the structure of the scanning identification system, the end faces of one ends of the plurality of optical fibers may be arranged at intervals to form a fiber grating.

Through patent retrieval, the following patents have a certain relationship with the present application:

the utility model provides an application number is "202122013893.1", application date is "2021.08.25", publication number is "CN215910008U", publication date is "2022.02.25", the name is "multichannel optical fiber bundle and input optical spectrum beam splitting module that optical fiber array arranged", the utility model patent of applicant for "west ampere and its opto-electrical technology limited company", the utility model provides a multichannel optical fiber bundle and input optical spectrum beam splitting module that optical fiber array arranged, it is limited to solve the extension of the passageway quantity that current single instrument multichannel was measured, and the passageway counts excessively, the signal of optical fiber transmission surpasss image plane field of view scope, lead to the problem that can't detect. The optical fiber bundle comprises a positioning sheet and a plurality of optical fibers; the positioning sheet comprises an upper positioning sheet body and a lower positioning sheet body which have the same structure, a first groove is formed in the upper positioning sheet body, and a second groove is formed in the lower positioning sheet body; the first groove and the second groove have the same structure; after the upper positioning sheet body and the lower positioning sheet body are buckled, the first groove and the second groove form accommodating grooves of the optical fibers; all the optical fibers are arranged in an array in the accommodating groove; the outer contour of the buckled upper positioning sheet body and the buckled lower positioning sheet body is circular. However, the utility model is to arrange the optical fibers in a matrix, which is used to solve the problems of the existing single instrument and the excessive number of channels, and needs to be additionally provided with gratings. The utility model does not disclose the structure of the grouped straight grating, and can not be used for scanning and counting the optical signals.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to the defect that exists among the prior art, provide a fiber grating.

In order to solve the technical problem, the utility model discloses the technical scheme who takes does: a fiber grating. The end faces of one ends of a plurality of optical fibers are arranged at intervals, the end face of each optical fiber is used as a lighting point for collecting optical signals, and scanning and identification are carried out on an object to be detected on a detection plate to form an optical fiber grating. The mode of combining the grating and the optical fiber into a whole can reduce the transmission loss of optical signals, improve the detection sensitivity of the optical signals and enable the structure of a scanning recognition system to be more compact.

Furthermore, the number of the optical fibers is larger than or equal to the number of columns in the matrix of the objects to be detected on the detection plate. So that each row of the object to be detected in the matrix of the object to be detected has one optical fiber corresponding to the object to be detected. In the relative movement process of the detection plate and the grouped straight-line grating, the grouped straight-line grating scans and identifies all the objects to be detected.

Furthermore, the maximum size of the object to be detected is not less than 0.5 multiplied by the maximum size of the optical fiber, and the diameter of the optical fiber is not more than 1.5 multiplied by the maximum size of the object to be detected. The diameter of the optical fiber is equivalent to the size of the object to be detected, so that the optical fiber serving as a light collecting point can collect optical signals emitted by the aligned object to be detected, the interference of the optical signals emitted by the peripheral object to be detected can be avoided, and the accuracy and the detection precision of scanning identification are improved.

Furthermore, the end faces of one ends of the optical fibers are arranged at intervals along the grating direction, so that the end faces of the optical fibers serving as the lighting points correspond to a row of objects to be detected.

Further, the distance between adjacent lighting points = the distance between the matrixes to be detected. So that each fiber end face as a light collecting point can be aligned with one object to be inspected in one row.

Further, the pitch of adjacent lighting points = √ 2 × the pitch of the matrix of the objects to be detected. When the fiber bragg grating scans the detection board at an oblique angle of 45 degrees, the end face of each optical fiber can be respectively aligned with one object to be detected in the matrix of the object to be detected.

Furthermore, adjacent light collecting points are arranged in a staggered mode in the direction perpendicular to the grating direction. The optical fiber end faces serving as the lighting points correspond to two adjacent rows of to-be-detected objects in the matrix on the detection plate, so that the distance between the adjacent lighting points is increased, the optical interference emitted by the to-be-detected objects adjacent to the to-be-detected objects aligned to the lighting points is reduced or avoided, and the detection precision is improved.

Further, the adjacent lighting spot dislocation distance = the adjacent lighting spot spacing. All the optical fiber end faces as light collecting points can be aligned to two adjacent rows of objects to be detected in the objects to be detected on the detection plate.

Furthermore, one ends of the optical fibers are fixed in a bonding or clamping mode by a clamp to form a grouped in-line grating. One end of the optical fiber serving as the lighting point is kept fixed, and the lighting point can be aligned to each row of objects to be detected in the matrix of the objects to be detected during scanning and identification.

The utility model has the advantages that: the end faces of one ends of the optical fibers are arranged at intervals, the end face of each optical fiber is used as a lighting point for collecting optical signals, and scanning and identifying of the object to be detected on the detection plate are performed to form an optical fiber grating used for scanning and identifying of the object to be detected. The mode of combining the grating and the optical fiber into a whole can reduce the transmission loss of optical signals, improve the detection sensitivity of the optical signals and enable the structure of a scanning recognition system to be more compact.

Drawings

FIG. 1 is a schematic perspective view of a scanning recognition system,

figure 2 is a schematic front view of a scanning identification system,

figure 3 is a schematic view of a test strip,

FIG. 4 is a schematic diagram of a three-dimensional structure of a multi-fiber in-line grating,

figure 5 is a schematic diagram of a multi-fiber in-line grating,

FIG. 6 is a schematic diagram of a three-dimensional structure of a multi-fiber dislocation grating,

figure 7 is a schematic diagram of a multiple fiber dislocated grating,

figure 8 is a schematic view of a scan recognition process 1,

figure 9 is a schematic view of a scan recognition process 2,

fig. 10 is a schematic diagram of the scan identification process 3.

In the figure: 1-moving device, 2-detecting plate, 3-exciting light source, 4-grouping in-line grating, 41-multi-fiber in-line grating, 42-multi-fiber dislocation grating, 401-lighting point, 5-fiber, 6-identifying device, d-maximum size of the object to be detected, K-matrix spacing of the object to be detected, L-spacing of adjacent lighting points, M-spacing of adjacent lighting points, Q-fiber diameter, X-grating direction and Y-detecting plate moving direction.

Detailed Description

The invention will be further described by means of specific embodiments and with reference to the accompanying drawings:

as shown in fig. 1 and 2, the scanning recognition system of the present application includes: the device comprises a moving device 1, a detection plate 2, an excitation light source 3, a grouped straight grating 4 and a recognition device 6.

The grouped in-line grating 4 is composed of a plurality of light collecting spots 401 arranged at intervals in the grating direction X. The sampling point 401 is square, circular or elliptical. The number of the lighting points 401 of the grouped straight-row grating 4 is larger than or equal to the number of columns in the matrix of the to-be-detected objects 201 on the detection plate 2, so that each to-be-detected object 201 in each row corresponds to one lighting point 401. The maximum size of the light collecting point 401 should be within 0.5-1.5 times of the maximum size d of the object to be detected, so that the size of the light collecting point 401 is equivalent to that of the object to be detected 201. When the lighting point 401 is close to and aligned with the object to be detected 201, it is ensured that the light signal collected by the lighting point 401 is the light emitted by the aligned object to be detected 201, so that the interference of the light signal emitted by the peripheral object to be detected 201 is avoided, and the accuracy and detection precision of scanning identification are improved. Therefore, the grouped straight-line grating 4 cannot adopt a stripe grating, so as to prevent light emitted by the object to be detected 201 around the object to be detected 201, which is aligned with the light collecting point 401, from entering the light collecting point 401 and interfering with the detection precision.

The grouped straight line grating 4 is arranged at one side of the detection plate 2, the detection plate 2 is arranged on the mobile device 1, and the grouped straight line grating 4 can also be arranged on the mobile device 1, so that the detection plate 2 and the grouped straight line grating 4 form relative motion. The excitation light source 3 emits light to irradiate the detection plate 2, and the sample 201 to be detected having the fluorescent marker bound thereto emits light. The light emitted by the object 201 is transmitted to the identification device 6 after passing through the grouped straight-line grating 4, and the light-sensitive tube in the identification device 6 converts the light signal collected by the grouped straight-line grating 4 into an electric signal for operation or teletransmission display.

However, the identification device 6 in the scanning identification system needs to be arranged together with the grouped in-line grating 4, which results in a complex and bulky structure of the detection part. For this purpose, an optical fiber 5 is arranged between the grouped in-line grating 4 and the identification device 6, the optical signal collected by the grouped in-line grating 4 is transmitted to the identification device 6 through the optical fiber 5, and the position of the identification device 6 can be flexibly arranged, so that the structure of the detection part is simplified.

To further simplify the structure of the detection unit, the grouped in-line grating 4 may be combined with one end of the optical fiber 5. One end of a plurality of optical fibers 5 can be arranged linearly according to grouping intervals to form lighting points 401 arranged linearly according to grouping intervals, and a multi-fiber straight-line grating 41 or a multi-fiber staggered grating 42 can be formed; the diameter Q of the optical fiber is in the range of 0.5-1.5 times of the maximum dimension d of the object to be detected.

As shown in fig. 3, the objects 201 are arranged on the detection plate 2 in a matrix with a matrix pitch K. The sample 201 includes various molecules of the substance to be analyzed, such as chemical analysis, protein analysis, nucleic acid analysis, cell analysis, exosome analysis, circulating tumor cell analysis, and nanomaterial analysis, and can be used in the fields of precise medical treatment, forensic identification, food safety, and environmental protection. A part of the sample 201 is bound with a fluorescent label. After the light emitted from the excitation light source 3 irradiates the sample 201, the fluorescence marker on the sample 201 is excited to fluoresce.

Embodiment 1 as shown in fig. 4 and 5, the multi-fiber in-line grating 41 is used as the grouped in-line grating 4, and one end of the optical fiber 5 is used as the light collecting point 401, and one ends of the plurality of optical fibers 5 are arranged in a straight line at the pitch L. The distance L between adjacent light sampling points is the same as the distance K between the matrixes to be detected, or is V2 times of the distance K between the matrixes to be detected. The fluorescence on the object 201 is collected by one end of the optical fiber 5, transmitted to the other end of the optical fiber 5 through the optical fiber 5, and converted into an electric signal by the photosensitive tube in the identification device 6.

In embodiment 2, as shown in fig. 6 and 7, the multi-fiber shifted grating 42 is used as the in-line grouped grating 4, in which one ends of a plurality of optical fibers 5 are arranged in the grating direction X, and one ends of adjacent optical fibers 5 are arranged in a shifted manner in the direction perpendicular to the grating direction X. The distance L between adjacent lighting points is the same as the distance K between the matrixes to be detected, and the dislocation distance M between adjacent lighting points is the same as the distance K between the matrixes to be detected or is 2 times of the distance K between the matrixes to be detected. One end of the optical fiber 5 of the multi-fiber shift grating 42, that is, the lighting point 401 of the multi-fiber shift grating 42 is made to correspond to the two rows of the objects 201 on the detection plate 2. The staggered arrangement mode can increase the distance between the adjacent light collecting points 401, so as to reduce or avoid the light interference of the to-be-detected objects 201 adjacent to the to-be-detected objects 201 aligned with the light collecting points 401, and improve the detection precision.

The scanning identification process 1 of the scanning identification method of grouped straight-line grating of the present application is shown in fig. 8, and the moving device 1 drives the detection plate 2 to form a relative motion with respect to the grouped straight-line grating 4. The light collecting points 401 on the multi-fiber straight grating 41 correspond to the positions of the objects 201 to be inspected distributed on the detection plate 2 in a matrix. At the same time, the light emitted from the excitation light source 3 is irradiated on the detection plate 2, and the sample 201 with the fluorescence label bound to the excitation portion emits light. When the lighting points 401 are aligned with the first row of the object to be detected 201, the light signals emitted by the object to be detected 201 emitting light in the first row are collected and transmitted to the identification device 6 through the optical fiber 5, and the light sensitive tube in the identification device 6 converts the light signals collected by the grouped in-line grating 4 into electric signals for counting. In the relative movement process of the detection plate 2 and the grouped straight-line grating 4, the light collecting points 401 on the multi-fiber straight-line grating 41 sequentially collect the light signals sent by each row of the objects to be detected 201 on the detection plate 2, and count the number of the objects to be detected 201 which emit light in the detection plate 2, so that the objects to be detected 201 are dynamically and continuously counted, the detection precision is improved, the identification time is reduced, and the detection efficiency is improved.

The scanning identification process 2 of the method for scanning identification of grouped in-line gratings is shown in fig. 9, and the grating direction X and the moving direction Y of the detection plate form an oblique angle of 45 °. At this time, the pitch L = √ 2 × the pitch K of the sample matrix is such that each sampling point 401 on the multi-fiber straight grating 41 corresponds to each sample 201 in the 45 ° direction in the sample 201 matrix on the detection plate 2. In the relative movement process of the detection plate 2 and the grouped straight-line grating 4, the light collecting points 401 on the multi-fiber straight-line grating 41 sequentially collect the light signals emitted by each row of the to-be-detected objects 201 on the detection plate 2, and count the number of the to-be-detected objects 201 emitting light in the detection plate 2, so that the to-be-detected objects 201 are counted dynamically and continuously. By the detection mode, the distance between the adjacent light collecting points 401 can be increased, the light interference of the to-be-detected object 201 adjacent to the to-be-detected object 201 aligned with the light collecting points 401 is reduced or avoided, and the detection precision is improved.

The scanning identification process 3 of the grouped in-line grating is shown in fig. 10, and the grouped in-line grating 4 adopts a multi-fiber dislocation grating 42. The lighting points 401 in the multi-fiber shifted grating 42 correspond to the positions of two rows of the objects 201 to be inspected distributed on the detection plate 2 in a matrix. When the lattice jump of the light collecting point 401 at the front position is aligned with one half of the first row of the objects to be detected 201, the light signals in the one half of the first row of the objects to be detected 201 are collected and transmitted to the identification device 6 through the optical fiber 5; when the leading daylighting point 401 is aligned with half of the second row of test objects 201, the trailing daylighting point 401 will be aligned with the other half of the test objects 201 in the first row of test objects 201 that have not yet been scanned. In the relative movement process of the detection plate 2 and the grouped straight-line grating 4, the light collecting points 401 on the multi-fiber dislocation grating 42 can sequentially collect the light signals sent by each row of the objects to be detected 201 on the detection plate 2, and count the number of the luminous objects to be detected 201 in the detection plate 2, so that the dynamic continuous counting of the objects to be detected 201 is realized. The lighting points 401 in the multi-fiber dislocation grating 42 correspond to two adjacent rows of the objects to be detected 201 in the matrix on the detection plate 2, so as to increase the distance between the adjacent lighting points 401, reduce or avoid the optical interference of the adjacent objects to be detected 201 of the objects to be detected 201 aligned with the lighting points 401, and improve the detection precision.

To sum up, the beneficial effects of the utility model are that: the end faces of one ends of the optical fibers are arranged at intervals, the end face of each optical fiber is used as a lighting point for collecting optical signals, and scanning and identifying are carried out on the object to be detected on the detection plate to form an optical fiber grating which is used for scanning and identifying the object to be detected 201. The mode of combining the grating and the optical fiber into a whole can reduce the transmission loss of optical signals, improve the detection sensitivity of the optical signals and enable the structure of a scanning recognition system to be more compact.

The above embodiments are provided only for the purpose of illustration, not for the limitation of the present invention, and those skilled in the relevant art can make various changes or modifications without departing from the spirit and scope of the present invention, therefore all equivalent technical solutions should also belong to the protection scope of the present invention, and the protection scope of the present invention should be defined by each claim.

Claims

1. A fiber grating, comprising: the end faces of one ends of a plurality of optical fibers (5) are arranged at intervals, the end face of each optical fiber (5) is used as a lighting point (401) for collecting optical signals, and the object (201) to be detected on the detection plate (2) is scanned and identified to form an optical fiber grating.

2. A fiber grating according to claim 1, wherein: the number of the optical fibers (5) is more than or equal to the number of columns in the matrix of the objects to be detected (201) on the detection plate (2).

3. A fiber grating according to claim 2, wherein: 0.5 times the maximum dimension (d) of the object to be inspected, less than or equal to the diameter (Q) of the optical fiber, less than or equal to 1.5 times the maximum dimension (d) of the object to be inspected.

4. A fiber grating according to claim 3, wherein: the end faces of one ends of the optical fibers (5) are arranged at intervals in the grating direction (X).

5. The fiber grating of claim 4, wherein: the distance (L) between adjacent lighting points is not less than the distance (K) between the matrixes of the objects to be detected.

6. The fiber grating of claim 4, wherein: the distance (L) = √ 2 × the distance (K) between the adjacent lighting points.

7. A fiber grating according to claim 3, wherein: the adjacent light collecting points (401) are arranged in a staggered manner in the direction perpendicular to the grating direction (X).

8. The fiber grating of claim 7, wherein: the adjacent lighting spot dislocation distance (M) = the adjacent lighting spot spacing (L).

9. A fiber grating according to any one of claims 1 to 8, wherein: one ends of a plurality of optical fibers (5) are fixed in a bonding or clamping mode to form a grouped in-line grating (4).