CN217909063U - Automatic chromatography filter device - Google Patents
Automatic chromatography filter device Download PDFInfo
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- CN217909063U CN217909063U CN202221501620.XU CN202221501620U CN217909063U CN 217909063 U CN217909063 U CN 217909063U CN 202221501620 U CN202221501620 U CN 202221501620U CN 217909063 U CN217909063 U CN 217909063U
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Abstract
The utility model discloses an automatic chromatographic filter device, which comprises a chromatographic column body, a feeding container, a collecting container, a vacuum pump and an inert gas tank, wherein the chromatographic column body comprises a packing cavity, a first valve arranged at the bottom and a packing inlet which can be opened and closed, and the first valve is communicated with the packing cavity; the feeding container is detachably arranged at the top of the chromatographic column body and is communicated with the packing cavity in a sealing way, and the top of the feeding container is provided with a feeding port which can be opened and closed; the collecting container is communicated with the first valve in a sealing way; the inlet end of the vacuum pump is communicated with the packing cavity; and the outlet end of the inert gas tank is respectively communicated with the top of the charging container, the top of the filling cavity and the collecting container, and a second valve is arranged outside the outlet end of the inert gas tank. The utility model discloses can let the material carry out the chromatography under anaerobic environment and filter, avoid the oxidation.
Description
Technical Field
The utility model relates to a chemical industry equipment technical field, concretely relates to automatic change chromatography filter equipment.
Background
Gel filtration chromatography is a technique for elution separation based on the difference in relative molecular mass of each component in a sample to be separated, using the molecular sieve action of particles having a porous network structure.
Gel filtration chromatography (gel filtration chromatography) is also called exclusion chromatography or molecular sieve method, and mainly separates and purifies proteins according to their size and shape, i.e. their mass. The packing material in the chromatographic column is some inert porous reticular structure material, mostly cross-linked glycan (such as dextran or agarose) material, so that the materials in the protein mixture are separated according to different molecular sizes. Also known as size exclusion chromatography (molecular-exclusion chromatography). A chromatographic technique for separating protein or other molecular mixture according to molecular size by using gel beads with holes as matrix. Generally, the large molecules flow out first, and the small molecules flow out later.
In the related art, some materials to be filtered are easily oxidized in the air, and the current chromatographic filtering device lacks the design for chromatographic filtering in an anaerobic environment.
SUMMERY OF THE UTILITY MODEL
The utility model provides an automatic chromatography filter equipment can let the material carry out the chromatography under anaerobic environment and filter.
The utility model discloses a following technical scheme realizes:
an automatic chromatographic filter device comprises a chromatographic column body, a feeding container, a collecting container, a vacuum pump and an inert gas tank, wherein the chromatographic column body comprises a packing cavity, a first valve arranged at the bottom and an openable packing inlet, and the first valve is communicated with the packing cavity; the feeding container is detachably arranged at the top of the chromatographic column body and is hermetically communicated with the packing cavity, and the top of the feeding container is provided with a feeding port which can be opened and closed; the collecting container is communicated with the first valve in a sealing way; the inlet end of the vacuum pump is communicated with the packing cavity; the outlet end of the inert gas tank is communicated with the top of the charging container, the top of the filling cavity and the collecting container respectively, and a second valve is arranged outside the outlet end of the inert gas tank.
The working principle of the utility model is as follows:
before the chromatography, with reinforced container, the airtight intercommunication in inside of collection container and packing chamber earlier, then take out inside air through the vacuum pump, open the second valve after having taken out, with the inert gas release in the inert gas jar, in order being full of the inner space, then will pack through the filler entry add chromatography column body, during the material added reinforced container, alright carry out the chromatography and filter, whole inner space is full of inert gas, can let the material carry out the chromatography under anaerobic environment and filter, avoid the oxidation.
Further, the bottom of the charging container is provided with a third valve.
Through setting up the third valve, be convenient for control reinforced quantity.
Further, an air pump is arranged between the outlet end of the inert gas tank and the filling cavity.
Through setting up the air pump, can regulate and control the pressure in the packing chamber better to adjust chromatography speed.
Further, a pressure sensor is arranged in the packing cavity.
Through setting up pressure sensor, can be according to the pressure that pressure sensor shows, the pressure size in the regulation condiment chamber more accurately.
Further, the automatic chromatography filter device also comprises a controller, wherein the input end of the controller is connected with the pressure sensor, and the output end of the controller is communicated with the air pump.
Through setting up the controller, the pressure that pressure sensor transmitted is received to the controller to through the rotational speed of control air pump automatic with this pressure regulation and control to preset pressure, realize automatic regulation and control.
Furthermore, the feeding container and the chromatographic column body are made of glass materials.
Through this arrangement, it is convenient to observe the chromatography process.
Further, the automatic chromatography filter device also comprises a frame body, and the chromatography column body, the feeding container, the vacuum pump and the inert gas tank are all installed on the frame body.
Compared with the prior art, the utility model, following advantage and beneficial effect have:
the utility model discloses an automatic chromatography filter equipment includes chromatography column body, reinforced container, collection container, vacuum pump and inert gas jar, replaces inside air for inert gas before the chromatography to build anaerobic environment in inside, make the material carry out the chromatography under anaerobic environment and filter, avoid oxidizing.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
fig. 1 is a schematic structural diagram of an automatic chromatography filtering apparatus according to an embodiment of the present invention.
Reference numbers and corresponding part names in the drawings:
10-a chromatography column body; 11-a packing chamber; 12-a first valve; 13-filler inlet; 14-a pressure sensor; 20-a feeding container; 21-a feed inlet; 22-a third valve; 30-a collection container; 40-a vacuum pump; 50-inert gas tank; 51-second valve.
Detailed Description
To make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the following examples and drawings, and the exemplary embodiments and descriptions thereof of the present invention are only used for explaining the present invention, and are not intended as limitations of the present invention.
Examples
As shown in FIG. 1, an automatic chromatographic filter device comprises a chromatographic column body 10, a feeding container 20, a collecting container 30, a vacuum pump 40 and an inert gas tank 50, wherein the chromatographic column body 10 comprises a packing cavity 11, a first valve 12 arranged at the bottom and an openable packing inlet 13, and the first valve 12 is communicated with the packing cavity 11. The feeding container 20 is detachably mounted at the top of the chromatographic column body 10 and is hermetically communicated with the packing cavity 11, and the top of the feeding container 20 is provided with an openable feeding port 21; the collection container 30 is in sealed communication with the first valve 12. The inlet end of the vacuum pump 40 is in communication with the packing chamber 11. The outlet end of the inert gas tank 50 is respectively communicated with the top of the charging container 20, the top of the filling cavity 11 and the collecting container 30, and a second valve 51 is arranged outside the outlet end of the inert gas tank 50.
The working principle of the utility model is as follows:
before the chromatography, with reinforced container 20 earlier, the inside airtight intercommunication of collecting vessel 30 and filled cavity 11, then take out inside air through vacuum pump 40, open second valve 51 after taking out, release the inert gas in inert gas jar 50, with being full of inner space, then through packing entry 13 with pack add in chromatography column body 10, the material is added in reinforced container 20, alright carry out the chromatography and filter, whole inner space is full of inert gas, can let the material carry out the chromatography under anaerobic environment and filter, avoid the oxidation.
In a further embodiment, the bottom of the charging container 20 is provided with a third valve 22. The third valve 22 is arranged, so that the dosage of the feeding materials can be conveniently controlled.
In another embodiment, a gas pump is provided between the outlet end of the inert gas tank 50 and the filling chamber 11. By arranging the air pump, the pressure in the packing cavity 11 can be better regulated and controlled to adjust the chromatography speed.
In a further embodiment, a pressure sensor 14 is provided in the packing chamber 11. Through setting up pressure sensor 14, can be according to the pressure that pressure sensor 14 shows, the pressure size in the condiment chamber is adjusted and control more accurately.
In a further embodiment, the automated chromatography filtration apparatus further comprises a controller, an input of the controller is connected with the pressure sensor 14, and an output of the controller is communicated with the air pump. By arranging the controller, the controller receives the pressure transmitted by the pressure sensor 14, and automatically regulates and controls the pressure to a preset pressure by controlling the rotating speed of the air pump, so that automatic regulation and control are realized.
In another embodiment, the feeding container 20 and the chromatography column body 10 are made of glass. Through this arrangement, it is convenient to observe the chromatography process.
In another embodiment, the automated chromatography filtration apparatus further comprises a frame body on which the chromatography column body 10, the charging container 20, the vacuum pump 40 and the inert gas tank 50 are mounted.
The above-mentioned embodiments, further detailed description of the objects, technical solutions and advantages of the present invention, it should be understood that the above description is only the embodiments of the present invention, and is not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (7)
1. An automatic chromatography filter device is characterized by comprising a chromatography column body, a feeding container, a collecting container, a vacuum pump and an inert gas tank, wherein the chromatography column body comprises a packing cavity, a first valve arranged at the bottom and an openable packing inlet, and the first valve is communicated with the packing cavity; the feeding container is detachably arranged at the top of the chromatographic column body and is communicated with the packing cavity in a sealing way, and the top of the feeding container is provided with a feeding port which can be opened and closed; the collecting container is communicated with the first valve in a sealing way; the inlet end of the vacuum pump is communicated with the packing cavity; the outlet end of the inert gas tank is communicated with the top of the charging container, the top of the filling cavity and the collecting container respectively, and a second valve is arranged outside the outlet end of the inert gas tank.
2. The automated chromatographic filtration device of claim 1, wherein the bottom of the feed vessel is provided with a third valve.
3. The automated chromatographic filter device of claim 1, wherein a gas pump is provided between the outlet end of the inert gas tank and the packing chamber.
4. The automated chromatographic filter device according to claim 3, wherein the packing chamber is provided with a pressure sensor therein.
5. The automated chromatographic filter device according to claim 4, further comprising a controller, wherein an input of the controller is connected to the pressure sensor, and an output of the controller is in communication with the air pump.
6. The automated chromatographic filter device according to any one of claims 1 to 5, wherein the feed container and the chromatographic column body are made of glass.
7. The automated chromatographic filter device according to any one of claims 1 to 5, further comprising a frame body on which the chromatographic column body, the feeding container, the vacuum pump and the inert gas tank are mounted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221501620.XU CN217909063U (en) | 2022-06-15 | 2022-06-15 | Automatic chromatography filter device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221501620.XU CN217909063U (en) | 2022-06-15 | 2022-06-15 | Automatic chromatography filter device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217909063U true CN217909063U (en) | 2022-11-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221501620.XU Active CN217909063U (en) | 2022-06-15 | 2022-06-15 | Automatic chromatography filter device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217909063U (en) |
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2022
- 2022-06-15 CN CN202221501620.XU patent/CN217909063U/en active Active
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