CN217878431U - Constant temperature sampling device - Google Patents

Abstract

The application discloses constant temperature sampling device belongs to microbial fermentation technical field. This constant temperature sampling device includes that sampling tube and cover locate the constant temperature sleeve outside the sampling tube, and the sampling tube is used for splendid attire fermentation liquid, and constant temperature sleeve includes outer wall, inner wall and is located the sealed cavity between outer wall and the inner wall, and the sealed cavity intussuseption is filled with phase change material. Utilize phase change material to carry out the heat exchange to fermentation liquid to make fermentation liquid can keep for a long time in the constant temperature who accords with PH buffering calibration detection temperature within range, make the measurement to fermentation liquid more accurate.

Description

Constant temperature sampling device

Technical Field

The application belongs to the technical field of microbial fermentation, especially, relate to a constant temperature sampling device.

Background

In the microbial fermentation process, the pH value of raw and auxiliary materials required by the biological fermentation of liquids such as fermentation metabolic liquid, fuel ethanol, on-line wine, polymeric ferric sulfate and the like is an important index for measuring the product quality and whether the operation is stable or not in the production process, the change of the pH value reflects the metabolic state of microorganisms, finished products accord with the national standard sale standard, and the raw and auxiliary materials accord with energy substances required by the growth of the microorganisms. Taking microbial fermentation as an example, for continuous fermentation process, pH is an important indicator of microbial growth in a reactive fermentation tank. In the process of continuous fermentation, there is the difference in the temperature in the different fermentation tanks, and the experimenter sample simultaneously and need be for a certain time (the temperature reduces soon in winter, and the temperature is too high in summer), receives fermentation cylinder temperature and ambient temperature influence, receives to detect the zymotic fluid and is less than or is higher than PH buffering calibration detection temperature range, causes experiment detection error. In order to ensure the correct pH value of a measured sample, the sample measurement and the standard pH buffer calibration are required to be ensured to be at the same temperature, so the temperature compensation and the slope compensation range are required to be carried out, but the detection temperature is not in the pH buffer calibration temperature range, the temperature adjustment is required to be carried out on the pH detection sample, temperature differences exist in different sampling environments of the same fermentation tank, or temperature differences exist in different fermentation tank samples at the same time, the pH detection is inaccurate, the inaccurate pH value detection result causes the production misjudgment of the reproductive metabolism condition of bacteria, and the health state of the bacteria, and the regulation and control of the strain fermentation process are seriously influenced.

SUMMERY OF THE UTILITY MODEL

The application aims at solving the technical problem that the temperature of the fermentation liquid after the current sampling is higher or lower than the detection temperature of the PH buffer calibration at least to a certain extent. To this end, the present application provides a constant temperature sampling device.

The embodiment of the application provides a constant temperature sampling device includes:

the sampling tube is used for containing fermentation liquid;

the constant temperature sleeve is sleeved outside the sampling pipe and comprises an outer wall, an inner wall and a sealed cavity between the outer wall and the inner wall, and the sealed cavity is filled with a phase-change material.

Alternatively, for better implementing the present application, the phase change material is an inorganic phase change material.

Optionally, in order to better implement the present application, the material of the inner wall and the outer wall is any one of glass, plastic and stainless steel.

Optionally, in order to better realize the present application, a cover for closing the opening is detachably connected to the opening of the sampling tube.

Optionally, for better realization of the present application, the cover body is in spiral sealing connection with the sampling tube.

Optionally, in order to better implement the present application, a temperature detection device for detecting the temperature of the fermentation liquid is fixed at the bottom end of the cover body.

Optionally, in order to better implement the present application, the temperature detecting device includes a temperature probe and a temperature display, a detecting end of the temperature probe is inserted into the fermentation solution, and the temperature display is electrically connected to the temperature probe to display the temperature measured by the temperature probe.

Optionally, for better realisation of the present application, a thermostatic sleeve is removably connected to the sampling tube.

Optionally, for better realisation of the present application, the inner diameter of the thermostatic sleeve is adapted to the outer diameter of the sampling tube.

Optionally, in order to better implement the present application, the cover body protrudes from the upper end of the thermostatic sleeve.

The utility model discloses compare in prior art and have following beneficial effect:

the utility model discloses a fermentation liquid that the sampling tube splendid attire was detected and is waited to use the constant temperature sleeve cover to establish outside the sampling tube, utilize the phase change material of packing to carry out the heat exchange to fermentation liquid between constant temperature sleeve outer wall and the inner wall, so that fermentation liquid can keep for a long time in the constancy temperature who accords with PH buffering calibration detection temperature range, make the measurement to fermentation liquid more accurate.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

FIG. 1 shows a schematic sectional structure of a constant temperature sampling apparatus;

fig. 2 shows a schematic structural view of the thermostatic cartridge of fig. 1.

Reference numerals:

10-a sampling tube; 11-a sampling cavity;

20-a thermostatic sleeve; 21-outer wall; 22-inner wall; 23-a phase change material; 24-a thermostatic chamber;

30-a cover body;

40-temperature detection means; 41-temperature probe; 42-temperature display.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without making creative efforts belong to the protection scope of the present invention.

It should be noted that all the directional indications in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, etc. in a certain posture, and if the certain posture is changed, the directional indication is changed accordingly.

In the present application, unless expressly stated or limited otherwise, the terms "connected" and "fixed" are to be construed broadly, e.g., "fixed" may be fixedly connected or detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In addition, descriptions in the present application as to "first", "second", and the like are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicit to the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

The application is described below with reference to specific embodiments in conjunction with the following drawings:

the embodiment of the application provides a constant temperature sampling device for to fermentation liquid take a sample and keep fermentation liquid's constancy of temperature, so that follow-up PH value measurement to fermentation liquid carries out.

As shown in fig. 1 and 2, the thermostatic sampling device comprises a sampling tube 10 and a thermostatic sleeve 20. The top of sampling tube 10 sets up the sample opening, and inside is equipped with the sample chamber with sample opening intercommunication, and the sample chamber of sampling tube 10 is used for the splendid attire to receive the fermentation liquid of getting from the fermentation cylinder. The top of the thermostatic sleeve 20 is provided with a thermostatic opening, and the inside of the thermostatic sleeve is provided with a thermostatic cavity communicated with the thermostatic opening. The sampling tube 10 is located in the thermostatic chamber, so that the thermostatic sleeve 20 is sleeved outside the sampling tube 10, and the thermostatic sleeve 20 covers the side wall and the bottom of the sampling tube 10. The thermostatic sleeve 20 comprises an outer wall 21, an inner wall 22 and a cavity between the outer wall 21 and the inner wall 22, wherein a phase-change material 23 is filled in the cavity, and the inner wall 22 forms a thermostatic cavity. After the sampling tube 10 is placed in the constant-temperature cavity of the constant-temperature sleeve 20, the constant temperature of the fermentation liquid is kept within a certain time by utilizing the heat storage performance of the phase-change material 23, so that the temperature of the obtained fermentation liquid can be maintained within the range of the PH buffering calibration detection temperature, and the accuracy of the measurement result of the fermentation liquid subsequently entering a PH measuring instrument is improved.

In the present embodiment, the sampling tube 10 is detachably connected to the thermostatic sleeve 20, the diameter of the thermostatic chamber of the thermostatic sleeve 20 is adapted to the outer diameter of the sampling tube 10, and the diameter of the thermostatic chamber of the thermostatic sleeve 20 is the inner diameter of the thermostatic sleeve 20. And the length of the thermostatic cavity of the thermostatic sleeve 20 is less than or equal to the length of the sampling tube 10, so that after the sampling tube 10 is placed in the thermostatic sleeve 20, the outer wall 21 of the sampling tube 10 can be attached to the inner wall 22 of the thermostatic sleeve 20, and a thermostatic effect can be better realized. Also, when the fermentation liquid is taken from the fermentation tank, the weight of the sampling tube 10 alone for taking the fermentation liquid is lighter. Of course, in some other embodiments, the sampling tube 10 and the thermostatic sleeve 20 may also be fixed as one piece.

In this embodiment, the phase transition temperature of the phase change material 23 is 20 ℃, so that the fermentation liquid after sampling can be maintained at 20 ℃ for a long time to satisfy the PH buffer calibration detection temperature range.

Preferably, the phase change material 23 is an inorganic phase change material 23, and the inorganic phase change material 23 has a better and stable constant temperature effect compared with the organic phase change material 23, so that the temperature of the fermentation liquid can be maintained within the range of the pH buffer calibration detection temperature. Specifically, the inorganic phase change material 23 used in this embodiment includes a composition containing, by mass, 32% of calcium chloride hexahydrate, 5% of glycerol, 4.5% of potassium persulfate, 4% of methyl vinyl acetate, 6.2% of acrylic acid, 2.8% of sodium chloride, and 45.5% of water, so that the inorganic phase change material 23 has non-toxic and non-corrosive characteristics. On the basis that the adopted inorganic phase change material 23 has the characteristics of no toxicity and no corrosion, the inner wall 22 and the outer wall 21 of the thermostatic sleeve 20 are made of any one of glass, plastic and stainless steel.

Further, the sampling opening part of the sampling tube 10 is detachably connected with a phase-change material 23, and the phase-change material 23 is used for sealing the sampling opening part of the sampling tube 10 and the sampling cavity of the sampling tube 10, so that direct heat exchange between the fermentation liquid and air is avoided, and the constant temperature performance of the constant temperature sampling device is improved.

In this embodiment, the phase-change material 23 is connected with the sampling tube 10 in a thread sealing manner, and the phase-change material 23 and the sampling tube 10 are respectively provided with a matching external thread and a matching internal thread, so as to realize the thread sealing connection. Of course, in some other embodiments, the sampling tube 10 and the phase change material 23 may be connected by plugging, and the phase change material 23 is in a plug shape and plugged into the sampling opening of the sampling tube 10 to be sealed and fixed.

Furthermore, a temperature detection device 40 is fixed on the phase change material 23, the temperature detection device 40 is used for detecting the temperature value of the fermentation liquid in the sampling tube 10 in real time, and an operator can judge whether the temperature of the fermentation liquid in the sampling tube 10 meets the temperature range of PH measurement according to the temperature value detected by the temperature detection device 40. If the temperature of the fermentation liquid is higher than the temperature range of PH measurement, the fermentation liquid needs to be continuously placed in a constant-temperature sampling device for continuous cooling, and if the temperature of the fermentation liquid is lower than the temperature range of PH measurement, the fermentation liquid needs to be heated.

Preferably, the temperature detecting device 40 comprises a temperature probe 41 and a temperature display 42, wherein the end of the phase change material 23 facing the bottom of the sampling tube 10 is the bottom end of the phase change material 23, the end opposite to the bottom end is the top end of the phase change material 23, the fixed end of the temperature probe 41 is fixed at the bottom end of the phase change material 23, and the detecting end of the temperature probe 41 faces the fermentation liquid in the sampling tube 10. The temperature display 42 is fixed on the top end of the phase change material 23, the temperature display 42 is electrically connected with the temperature probe 41, and a storage battery for supplying power to the temperature probe 41 and the temperature display 42 is arranged inside the phase change material 23. When the phase change material 23 is covered on the sampling opening of the sampling tube 10, the temperature probe 41 is inserted into the fermentation liquid in the sampling tube 10, and the temperature of the fermentation liquid is detected. The temperature value obtained by the temperature probe 41 is displayed by the temperature display 42, so that the operator can directly obtain the temperature of the fermentation liquid by the temperature display 42. Of course, the temperature probe 41 may be replaced by a temperature sensor for indirectly acquiring the temperature of the fermentation liquid by detecting the air temperature in the sampling cavity.

Of course, in some other embodiments, the temperature detection device 40 may also include a thermometer, by which the temperature of the fermentation liquid is obtained. However, the thermometer needs to be taken out for reading, and the temperature of the fermentation liquid cannot be rapidly obtained.

Further, the phase change material 23 protrudes from the upper end surface of the thermostatic sleeve 20, so that an operator can drive the sampling tube 10 to move by grabbing the cover 30, and the sampling tube 10 can be taken and placed.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples described in this specification can be combined and combined by those skilled in the art.

Claims (10)

1. A constant temperature sampling device, comprising:

the sampling tube is used for containing fermentation liquid;

the constant temperature sleeve is sleeved outside the sampling pipe and comprises an outer wall, an inner wall and a sealed cavity between the outer wall and the inner wall, and the sealed cavity is filled with a phase-change material.

2. A constant temperature sampling device according to claim 1, wherein the phase change material is an inorganic phase change material.

3. A constant temperature sampling device according to claim 1, wherein the inner and outer walls are made of any one of glass, plastic and stainless steel.

4. The device of claim 1, wherein a cover is detachably connected to the opening of the sampling tube for closing the opening.

5. A constant temperature sampling device as claimed in claim 4, wherein the cover is in screw sealing engagement with the sampling tube.

6. The constant-temperature sampling device according to claim 4, wherein a temperature detection device for detecting the temperature of the fermentation liquid is fixed at the bottom end of the cover body.

7. A constant temperature sampling device according to claim 6, wherein the temperature detection device comprises a temperature probe, the probe end of which is inserted into the fermentation liquid, and a temperature display which is electrically connected with the temperature probe to display the temperature measured by the temperature probe.

8. A thermostatic sampling device according to claim 1 wherein a thermostatic sleeve is removably attached to the sampling tube.

9. A thermostatic sampling device according to claim 8 wherein the inner diameter of the thermostatic sleeve is adapted to the outer diameter of the sampling tube.

10. A thermostatic sampling device according to claim 4 wherein the cover projects above the upper end of the thermostatic sleeve.

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