CN217868880U - A431 cell low-concentration hypocrellin A pretreatment device - Google Patents
A431 cell low-concentration hypocrellin A pretreatment device Download PDFInfo
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- CN217868880U CN217868880U CN202220794470.XU CN202220794470U CN217868880U CN 217868880 U CN217868880 U CN 217868880U CN 202220794470 U CN202220794470 U CN 202220794470U CN 217868880 U CN217868880 U CN 217868880U
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Abstract
The utility model provides a low concentration hypocrellin A preprocessing device of A431 cell. The A431 cell low-concentration hypocrellin A pretreatment device comprises: the inner part of the mounting frame is divided into a plurality of soaking cavities by partition plates, and pretreatment liquid is filled in the soaking cavities; the adjusting mechanism comprises an adjusting motor, a rotating shaft and a spoiler, the rotating shaft is fixedly mounted at the shaft end of the adjusting motor and penetrates through the mounting frame, and the spoiler is fixedly mounted on the rotating shaft. The utility model provides a low concentration hypocrellin A preprocessing device of A431 cell conveniently drives the link that the adjustment cover was gone up and down to adjust through first extensible member wholly, is equipped with the cell tissue that is used for the preliminary treatment on the installation tray, and the link conveniently drives the inside that the installation tray stretched into pretreatment liquid, can realize multiunit cell tissue soaking simultaneously and preliminary treatment in pretreatment liquid under the different concentration environment.
Description
Technical Field
The utility model relates to a cell experiment technical field especially relates to a low concentration hypocrellin A preprocessing device of A431 cell.
Background
However, with the accelerated aging process of population, the increased ultraviolet exposure caused by ozone depletion and other factors, the growth of the human body is extremely rapid, the onset age of the human body is younger, and the squamous cell carcinoma becomes one of the most rapid-growing tumors in malignant tumors in China.
As the incidence of squamous cell carcinoma has been increasing and the destruction is high in recent years, there has been a growing interest in the pathogenesis and treatment of squamous cell carcinoma. Primary squamous carcinoma is rare, secondary squamous carcinoma is common, and the primary squamous carcinoma is usually caused by canceration based on the injury of original chronic skin diseases, scars and the like. The squamous cell carcinoma is discovered and treated in an early stage, and the cure rate is high; however, once the growth phase is in the rapid growth phase, the cells can rapidly transfer and invade a plurality of organs of the organism, even systemic transfer occurs, and the cells are large in destructiveness and poor in prognosis.
In research on pathogenesis and treatment of squamous cell carcinoma, particularly in immunotherapy and targeted therapy, a series of favorable results are obtained, but formation and transfer of squamous cell carcinoma are regulated by a plurality of factors such as cell proliferation, differentiation, growth factors, signal channels and the like, so that the formation and transfer of squamous cell carcinoma are extremely complex regulation and control processes, the pathogenesis of squamous cell carcinoma is not completely clarified at present, in a human skin squamous cell carcinoma A431, the cell activity can be reduced by pretreating the hypocrellin A with low dose, and simultaneously irradiating the cell with LED red light.
Therefore, it is necessary to provide a pretreatment apparatus for hypocrellin A with low concentration for A431 cells to solve the above technical problems.
SUMMERY OF THE UTILITY MODEL
The utility model provides a low concentration hypocrellin A preprocessing device of A431 cell has solved the inconvenient problem of carrying out the multiunit contrast experiment simultaneously of low concentration hypocrellin A preprocessing device.
In order to solve the technical problem, the utility model provides a low concentration hypocrellin A preprocessing device of A431 cell, include:
the inner part of the mounting frame is divided into a plurality of soaking cavities by partition plates, and pretreatment liquid is filled in the soaking cavities;
the adjusting mechanism comprises an adjusting motor, a rotating shaft and a spoiler, the rotating shaft is fixedly mounted at the shaft end of the adjusting motor and penetrates through the mounting frame, the spoiler is fixedly mounted on the rotating shaft and is positioned in the soaking cavity;
the first telescopic piece is fixedly arranged on the mounting frame;
the fixed plate is fixedly arranged at the shaft end of the first telescopic piece;
the adjusting cover is fixedly arranged on the fixing plate;
the connecting frame is fixedly arranged in the adjusting cover, and the connecting frame is provided with an installation tray.
Preferably, the adjusting motor is installed on the mounting frame, and the rotating shaft is rotatably connected with the mounting frame.
Preferably, the shaft end of the rotating shaft penetrates through the partition plate, and the rotating shaft is rotatably connected with the partition plate.
Preferably, the adjusting cover is sleeved on the mounting frame, and the adjusting cover is abutted to the mounting frame.
Preferably, the adjusting cover is of a transparent structure and is installed right above the installation frame.
Preferably, install on the adjustment cover and shelter from the mechanism, shelter from the mechanism and include the second extensible member, the axle head fixed mounting of second extensible member has and shelters from the net, install the rotating electrical machines on the link, the axle head of rotating electrical machines runs through the link, the axle head of rotating electrical machines with installation tray fixed connection, installation tray movable mounting in the top of link, the rotating electrical machines installs waterproof dustcoat outward.
Preferably, the shielding net is a glass net cover, and the thickness of the shielding net is 5-10 mm.
Preferably, the waterproof outer cover is a glass outer cover, and the surface of the waterproof outer cover is of a transparent structure.
Compared with the prior art, the utility model provides a low concentration hypocrellin A preprocessing device of A431 cell has following beneficial effect:
the utility model provides a low concentration hypocrellin A preprocessing device of A431 cell conveniently drives the link that the regulation was covered through first extensible member and wholly carries out the lift adjustment, is equipped with the cell tissue that is used for the preliminary treatment on the installation tray, and the link conveniently drives the inside that the installation tray stretched into preliminary treatment liquid, can realize soaking and preliminary treatment simultaneously of multiunit cell tissue in the preliminary treatment liquid under the different concentration environment.
Drawings
FIG. 1 is a schematic structural diagram of a first embodiment of a pretreatment device for hypocrellin A with A431 cells according to the present invention;
FIG. 2 is a schematic structural view of the whole shown in FIG. 1;
FIG. 3 is a schematic structural diagram of a second embodiment of the A431 cell hypocrellin A pretreatment device;
FIG. 4 is a three-dimensional view of the portion of the shielding mechanism shown in FIG. 3;
fig. 5 is a schematic structural view of the connecting frame portion shown in fig. 4.
The reference numbers in the figures:
1. the device comprises a mounting rack 11, a partition plate 101 and a soaking cavity;
2. the device comprises an adjusting mechanism 21, an adjusting motor 22, a rotating shaft 23 and a spoiler;
3. a first telescoping member;
4. a fixing plate;
5. an adjustment cover;
6. a connecting frame 61, a mounting tray;
7. a shielding mechanism 71, a second telescopic piece 72 and a shielding net;
8. rotating electrical machines, 81, waterproof housing.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and embodiments.
The first embodiment:
please refer to fig. 1 and fig. 2, wherein fig. 1 is a schematic structural diagram of a first embodiment of a pretreatment apparatus for hypocrellin a with a low concentration in a431 cell according to the present invention; fig. 2 is a schematic view of the overall structure shown in fig. 1.
A low concentration hypocrellin A pretreatment device of A431 cells comprises: the device comprises an installation rack 1, wherein a plurality of soaking cavities 101 are separated from the inside of the installation rack 1 through partition plates 11, and pretreatment liquid is filled in the soaking cavities 101;
the adjusting mechanism 2 comprises an adjusting motor 21, a rotating shaft 22 and a spoiler 23, the rotating shaft 22 is fixedly installed at the shaft end of the adjusting motor 21, the rotating shaft 22 penetrates through the mounting frame 1, the spoiler 23 is fixedly installed on the rotating shaft 22, and the spoiler 23 is located inside the soaking cavity 101;
the first telescopic piece 3 is fixedly arranged on the mounting frame 1;
the fixing plate 4 is fixedly arranged at the shaft end of the first telescopic part 3;
the adjusting cover 5 is fixedly arranged on the fixing plate 4;
and the connecting frame 6 is fixedly arranged in the adjusting cover 5, and the connecting frame 6 is provided with an installation tray 61.
Conveniently drive the link 6 on the adjusting cover 5 through first extensible member 3 and wholly carry out lift adjustment, be equipped with the cell tissue that is used for the preliminary treatment on the installation tray 61, link 6 conveniently drives the inside that installation tray 61 stretched into pretreatment liquid, realizes soaking and the preliminary treatment of cell.
The cell tissue is a431 cells.
The pretreatment solution adopts hypocrellin A with low concentration, and at least four groups of different concentrations are provided during filling;
at least three groups of partition plates 11 are arranged to form four soaking cavities 101, and the interior of each soaking cavity 101 is filled with hypocrellin A with different concentrations, so as to facilitate control experiments and pretreatment.
The first telescopic part 3 adopts a driving telescopic rod and is connected with an external power supply during use, so that a power source is provided for the lifting adjustment of the adjusting cover 5.
The adjusting motor 21 is installed on the mounting rack 1, and the rotating shaft 22 is rotatably connected with the mounting rack 1.
When the adjusting motor 21 is used, the adjusting motor is connected with an external power supply to provide a power source for the rotation of the rotating shaft 22, the rotating shaft 22 is at least provided with four groups of spoilers 23, and the rotating shaft 22 can drive the spoilers 23 to rotate and adjust;
at least four groups of spoilers 23 are arranged, one group of spoilers 23 corresponds to one soaking cavity 101 and is used for slowly stirring the low-concentration hypocrellin A to form the effect of flowing soaking pretreatment, so that the soaking stability and the cell surface contact sufficiency are improved.
The shaft end of the rotating shaft 22 penetrates through the partition plate 11, and the rotating shaft 22 is rotatably connected with the partition plate 11.
The adjusting cover 5 is sleeved on the mounting frame 1, and the adjusting cover 5 is abutted to the mounting frame 1.
The adjusting cover 5 is of a transparent structure, and the adjusting cover 5 is arranged right above the mounting rack 1.
The adjusting cover 5 provides support for the installation of the connecting frame 6, and the state in the pretreatment process is conveniently observed through a transparent structure.
The utility model provides a low concentration hypocrellin A preprocessing device of A431 cell's theory of operation as follows:
when the A431 cells need to be pretreated, the adjusting cover 5 is preferentially opened, and pretreatment liquid of hypocrellin A with low concentration is injected into the soaking cavity 101;
the A431 cells are placed above the corresponding mounting tray 61, the first telescopic piece 3 is started, the first telescopic piece 3 drives the adjusting cover 5 to move downwards through the fixing plate 4, and the adjusting cover 5 drives the A431 cells on the mounting tray 61 to move downwards and immerse the A431 cells into the pretreatment liquid.
Compared with the prior art, the utility model provides a low concentration hypocrellin A preprocessing device of A431 cell has following beneficial effect:
through the convenient link 6 that drives on the adjusting cover 5 of first extensible member 3 whole carry out lift adjustment, be equipped with the cell tissue that is used for the preliminary treatment on the installation tray 61, link 6 conveniently drives the inside that installation tray 61 stretched into pretreatment liquid, can realize the simultaneous soaking of multiunit cell tissue and preliminary treatment in pretreatment liquid under the different concentration environment.
The second embodiment:
referring to fig. 3, 4 and 5, a second embodiment of the present application provides another apparatus for pre-treating a431 cell with hypocrellin a based on the apparatus for pre-treating a431 cell with hypocrellin a according to the first embodiment of the present application. The second embodiment is only a preferred mode of the first embodiment, and the implementation of the second embodiment does not affect the implementation of the first embodiment alone.
Specifically, the difference between the low-concentration hypocrellin a pretreatment device for a431 cells provided in the second embodiment of the present application is that:
install on the adjustment cover 5 and shelter from mechanism 7, it includes second extensible member 71 to shelter from mechanism 7, the axle head fixed mounting of second extensible member 71 has and shelters from net 72, install rotating electrical machines 8 on the link 6, the axle head of rotating electrical machines 8 runs through link 6, the axle head of rotating electrical machines 8 with installation tray 61 fixed connection, installation tray 61 movable mounting in the top of link 6, rotating electrical machines 8 installs waterproof dustcoat 81 outward.
Through installing installation tray 61 at the output of rotating electrical machines 8, rotating electrical machines 8 conveniently drive installation tray 61 and carry out rotation regulation, and cooperation vortex structure makes to soak more abundant, and the waterproof dustcoat 81 of telescopic conveniently shelters from and protects the cell tissue of installation tray 61 top, can allow the circulation of preliminary treatment solution and prevent the slip of cell tissue.
The second telescopic part 71 is an electric telescopic rod, and is connected with an external power supply when in use, so as to provide a power source for the lifting adjustment of the shielding net 72.
The shielding net 72 is a glass net cover, and the thickness is 5-10 mm.
The shielding net 72 can stably shield and protect the cell tissue above the mounting tray 61, and prevent the cell tissue from falling off in the process of reaction solution turbulence.
The waterproof outer cover 81 is a glass outer cover, and the surface of the waterproof outer cover is of a transparent structure.
The waterproof housing 81 serves to protect the installation and use of the rotating electrical machine 8.
When in use, the rotating motor 8 is connected with an external power supply to provide a power source for the rotation adjustment of the mounting tray 61.
The utility model provides a theory of operation of second embodiment:
when the cells above the mounting tray 61 need to be shielded and protected, the second telescopic piece 71 is started, the second telescopic piece 71 drives the shielding net 72 to move downwards, and the shielding net 72 covers the mounting tray 61;
in the process of reaction solution turbulence, the rotating motor 8 is started, the rotating motor 8 drives the mounting tray 61 to rotate, and the mounting tray 61 drives the cell tissues on the mounting tray to turn.
The utility model provides a beneficial effect of second embodiment:
through installing installation tray 61 at the output of rotating electrical machines 8, rotating electrical machines 8 conveniently drive installation tray 61 and carry out rotation regulation, and cooperation vortex structure makes to soak more abundant, and the waterproof dustcoat 81 of telescopic conveniently shelters from and protects the cell tissue of installation tray 61 top, can allow the circulation of preliminary treatment solution and prevent the slip of cell tissue.
The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.
Claims (8)
1. A low concentration hypocrellin A preprocessing device of A431 cell, which is characterized in that the device comprises:
the device comprises a mounting frame, a plurality of soaking cavities are partitioned by partition plates in the mounting frame, and pretreatment liquid is filled in the soaking cavities;
the adjusting mechanism comprises an adjusting motor, a rotating shaft and a spoiler, the rotating shaft is fixedly mounted at the shaft end of the adjusting motor and penetrates through the mounting frame, the spoiler is fixedly mounted on the rotating shaft and is positioned inside the soaking cavity;
the first telescopic piece is fixedly arranged on the mounting frame;
the fixed plate is fixedly arranged at the shaft end of the first telescopic piece;
the adjusting cover is fixedly arranged on the fixing plate;
the connecting frame is fixedly arranged in the adjusting cover, and the connecting frame is provided with an installation tray.
2. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 1, wherein the adjusting motor is installed on the installation frame, and the rotation shaft is rotatably connected with the installation frame.
3. The pretreatment device for hypocrellin A431 cell according to claim 2, wherein the shaft end of the rotating shaft penetrates through the partition plate, and the rotating shaft is rotatably connected with the partition plate.
4. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 1, wherein said adjusting cover is sleeved on said mounting rack, and said adjusting cover abuts against said mounting rack.
5. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 4, wherein the adjusting cover is a transparent structure and is installed right above the installation rack.
6. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 1, wherein the adjusting cover is installed with a shielding mechanism, the shielding mechanism comprises a second expansion piece, the shaft end of the second expansion piece is fixedly installed with a shielding net, the connecting frame is installed with a rotating motor, the shaft end of the rotating motor penetrates through the connecting frame, the shaft end of the rotating motor is fixedly connected with the installation tray, the installation tray is movably installed above the connecting frame, and the rotating motor is externally installed with a waterproof outer cover.
7. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 6, wherein the shielding net is a glass net cover with a thickness of 5-10 mm.
8. The A431 cell low-concentration hypocrellin A pretreatment device according to claim 7, wherein the waterproof cover is a glass cover, and the surface of the waterproof cover is a transparent structure.
Priority Applications (1)
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CN202220794470.XU CN217868880U (en) | 2022-04-06 | 2022-04-06 | A431 cell low-concentration hypocrellin A pretreatment device |
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CN202220794470.XU CN217868880U (en) | 2022-04-06 | 2022-04-06 | A431 cell low-concentration hypocrellin A pretreatment device |
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CN217868880U true CN217868880U (en) | 2022-11-22 |
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CN202220794470.XU Active CN217868880U (en) | 2022-04-06 | 2022-04-06 | A431 cell low-concentration hypocrellin A pretreatment device |
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