CN217850688U

CN217850688U - Cover body of sterile tissue culture box and sterile tissue culture device

Info

Publication number: CN217850688U
Application number: CN202221176171.6U
Authority: CN
Inventors: 汪能定; 汪震亚
Original assignee: Zhejiang Zhenya Internet Of Things Technology Co ltd
Current assignee: Zhejiang Zhenya Internet Of Things Technology Co ltd
Priority date: 2022-05-12
Filing date: 2022-05-12
Publication date: 2022-11-22
Anticipated expiration: 2032-05-12

Abstract

The utility model discloses a cover body of box is banked up with earth to aseptic group for with be used for carrying on plant tissue culture's aseptic group banks up box intercombination with earth, thereby covers aseptic group banks up a first opening of box with earth, and includes: a conjunction, a filter piece and a removable piece that covers, wherein the conjunction is used for combining to aseptic group banks up the box with earth the first opening, just filter the piece and set up on the conjunction T and cover the second opening of combination frame. According to the utility model discloses a design, be equipped with a third opening on filtering the piece, just removable covering sets up filter on the piece and cover the third opening. According to this design, after the seedling is grown into to the tissue, if need carry out partial shipment or when processing to the seedling, operator or robotic arm only need open removable cover is in order to expose third opening on the filter piece, just accessible the third opening carries out relevant operation to reduce the seedling risk of infecting.

Description

Cover body of sterile tissue culture box and sterile tissue culture device

The technical field is as follows: the utility model relates to a technical field of plant tissue culture especially indicates a cover body and aseptic group of group's group banks up device with earth of aseptic group's box.

Background art:

plant tissue culture is known as a technique in which a part of cells or tissues of a plant body is separated from a mother body and cultured under appropriate conditions to enable growth, development, differentiation, and proliferation of the cells or tissues. The principle is that totipotent differentiation ability from plant cells, i.e., a certain class of cells in a plant body can develop independently and differentiate into an intact adult plant.

The operator familiar with plant tissue culture (commonly known in the art as "tissue culture") inevitably knows that the operation flow of tissue culture comprises the following steps:

(1) Taking the tissue/slice of the plant and putting the tissue/slice into a sterile plastic box or a flask (collectively called a sterile tissue culture box) for cultivation;

(2) After the seedlings are cultivated, the seedlings are transferred and concentrated into another sterile tissue culture box 11a (refer to a figure 1); and

(3) Waiting for the next stage of sub-packaging and seedling.

In other words, the sterile tissue culture cassette 11a shown in FIG. 1 is used for tissue culture of plants or waiting for dispensing of seedlings thereof.

In more detail, the tissue culture of plants must be performed in a low-bacteria/sterile environment (e.g., clean room) to ensure that the shoots grown by the tissue culture are not infected with bacteria. Unfortunately, practical experience has shown that during the process of sub-packaging and transporting the seedlings to another location, the seedlings are not in a sterile/low-bacteria environment and are therefore infected with bacteria. To solve this problem, as shown in fig. 2, the conventional method is to cover a sealing cover 12a directly on the aseptic tissue culture cassette 11a, thereby completely isolating the young seedling from the outside. However, the airtight sealing cap 12a rather makes the young seedlings housed inside the aseptic tissue culture cassette 11a unable to breathe. Moreover, the operator at the next stage needs to open the sealing cap 12a to perform the next stage of operation (e.g., sub-packaging and transporting) on the seedlings, which is very inconvenient.

In view of the foregoing reasons, the inventor of the present invention is the most intensely studied and created, and finally, develops and completes the cover body of the aseptic tissue culture box and the aseptic tissue culture device.

The utility model has the following contents:

a primary object of the present invention is to provide a cover body of aseptic group's tissue culture box for with be used for carrying out plant tissue culture's aseptic group to organize box intercombination, thereby cover aseptic group banks up a first opening of box with earth, and includes: a combining component, a filtering component and a detachable covering component, wherein the combining component (such as a combining frame) is used for being combined to the first opening of the sterile tissue culture box, and the filtering component is arranged on the combining component and covers the second opening of the combining frame. According to the utility model discloses a design, be equipped with a third opening on filtering the piece, just removable covering sets up filter on the piece and cover the third opening. According to this design, after the seedling is grown into to the tissue, if need carry out partial shipment or when processing to the seedling, operator or robotic arm only need open removable cover is in order to expose third opening on the filter piece, just accessible the third opening carries out relevant operation to reduce the seedling risk of infecting.

In order to achieve the above object, the present invention provides an embodiment of the cover body of the aseptic group banks up the box with earth, thereby it is used for and covers with aseptic group's group banks up box intercombination aseptic group banks up a first opening of box with earth, and includes:

a connecting member for connecting to the first opening and having a second opening;

the filter piece is arranged on the combining piece and covers the second opening, and a third opening is formed in the filter piece; and

a removable covering member disposed over the filter member and covering the third opening;

wherein, removable cover all have a filtration with filtering piece to have filtering capability.

In an embodiment, wherein the filter structure comprises:

an outer layer which is a non-woven fabric layer with the function of water repellent;

the middle layer is attached to the outer layer and is a melt-blown non-woven fabric layer capable of blocking dust haze, viruses and bacteria; and

and the inner layer is attached to the middle layer and is a composite fiber non-woven fabric layer.

In an embodiment, a coupling ring is disposed at the third opening, so that the detachable covering member is attached to the coupling ring through a felt adhesive member, and the detachable covering member is disposed on the coupling member and covers the second opening.

In an embodiment, a coupling ring is disposed at the third opening, and a caulking groove is disposed on a surface of the coupling ring.

In an embodiment, a bottom surface of the detachable covering member is provided with an embedding ring, and the detachable covering member is attached to the combining ring by embedding the embedding ring into the embedding groove, so that the detachable covering member is disposed on the combining member and covers the second opening.

And, the utility model discloses propose a sterile group simultaneously and organize device, wherein, include:

a sterile tissue culture box having a first opening; and

a cover, comprising:

a coupling member for coupling to the first opening of the sterile tissue culture cassette and having a second opening;

In an embodiment, wherein the filter structure comprises:

the middle layer is attached to the outer layer and is a melt-blown non-woven fabric layer capable of blocking dust haze, viruses and bacteria; and the inner layer is attached to the middle layer and is a composite fiber non-woven fabric layer.

In an embodiment, a connecting ring is disposed at the third opening, which facilitates the detachable covering member to be attached to the connecting ring through a felt adhesive member, so that the detachable covering member is disposed on the connecting member and covers the second opening.

In an embodiment, a coupling ring is disposed at the third opening, and a caulking groove is disposed on a surface of the coupling ring. And the bottom surface of the detachable covering part is provided with an embedded ring, and the detachable covering part is attached to the combining ring by embedding the embedded ring into the embedded groove, so that the detachable covering part is arranged on the combining part and covers the second opening.

Description of the drawings:

FIG. 1 is a perspective view of a known sterile tissue culture cassette;

FIG. 2 is a perspective view of a conventional sterile tissue culture cassette and a sealing lid;

FIG. 3 is an exploded view showing an aseptic tissue culture device according to the present invention;

FIG. 4 is a perspective view showing an aseptic tissue culture apparatus of the present invention;

FIG. 5 is a first perspective view of the cover body of the sterile tissue culture box of the present invention; and

fig. 6 is a second perspective view of the cover body of the aseptic tissue culture box of the utility model.

Reference numerals:

11a sterile tissue culture box

12a sealing cover

1: sterile tissue culture device

11, sterile tissue culture box

111 first opening

12: cover body

121: a coupling member

1211 a second opening

122 filter element

1221 third opening

123 removable covering member

1231 inserting ring

124 coupling ring

1241 caulking groove

The specific implementation mode is as follows:

in order to more clearly describe the cover body of the aseptic tissue culture box and the aseptic tissue culture device provided by the present invention, the following description will be made in detail with reference to the accompanying drawings.

Please refer to fig. 3, which shows an exploded view of an aseptic tissue culture device according to the present invention. In addition, fig. 4 is a perspective view of the sterile tissue culture device of the present invention. As shown in fig. 3 and 4, the aseptic tissue culture device 1 of the present invention comprises: the tissue culture device comprises a sterile tissue culture box 11 and a cover body 12, wherein the sterile tissue culture box 11 is provided with a first opening 111, and the cover body 12 is combined with the sterile tissue culture box 11 to cover the first opening 111.

As shown in fig. 3 and 4, the cover 12 includes: a connecting member 121, a filter member 122 and a removable covering member 123. In one embodiment, the connector 121 is, for example, a connecting frame having a second opening 1211, which is disposed on the sterile tissue culture cassette 11 to cover the sterile tissue culture cassette 11, and the second opening 1211 faces the first opening 111. It will be appreciated that the advantage of designing the coupling member 121 as a coupling frame is that it is strong and easy to couple to the sterile tissue culture cassette 11. Of course, in other possible embodiments, in order to ensure that the joint member 121 can be reliably and hermetically combined with the sterile tissue culture cassette 11, a sealing ring (i.e., an O-ring) may be disposed at the edge of the combining frame (i.e., the joint member 121). Alternatively, the connecting member 121 may be forced to be tightly combined with the sterile tissue culture box 11 by elastic band, belt, or buckle structure.

In particular, the present invention provides that the filter member 122 is disposed on the combining member 121 and covers the second opening 1211, and a third opening 1221 is disposed on the filter member 122. It is noted that fig. 3 and 4 illustrate the third opening 1221 as a small hole for passing only an operation tool (e.g., tweezers). Further, the present invention also provides that the detachable covering member 123 is disposed on the filtering member 122 and covers the third opening 1221.

It should be noted that the removable covering member 123 and the filter member 122 each have a filtering structure, thereby having a filtering function. In one embodiment, the filter structure comprises: the waterproof fabric comprises an outer layer, a middle layer and an inner layer, wherein the outer layer is a non-woven fabric layer with a water repellent function. On the other hand, the intermediate level laminating the skin, and be a melt-blown non-woven fabrics layer that can obstruct dust haze, virus and bacterium. Furthermore, the inner layer is attached to the middle layer and is a composite fiber non-woven fabric layer. It should be noted that, without considering the cost and the desire to enhance the antibacterial effect, the outer layer may be further covered with a nano-silicon layer, so that the nano-silicon layer is used as a sterilization layer to kill bacteria/viruses that would pass through the filtering structure, thereby achieving the effects of sterilization, bacteriostasis and deodorization.

Fig. 3 and 4 also show that the detachable covering member 123 has a tongue piece (tab). By this design, after the tissue is cultured into seedlings, if the seedlings need to be separated or processed, an operator or a mechanical arm can apply a force to the tongue piece of the removable covering member 123 to open the removable covering member 123 to expose the third opening 1221 (i.e., the small hole for operation) on the filter member 122, and then the related operation can be performed through the third opening 1221, so as to reduce the risk of infection of the seedlings.

In other words, the removable covering 123 needs to be attached/detached repeatedly many times. Therefore, in order to facilitate the attachment/detachment of the removable covering member 123 without damaging the surface layer of the filter member 122, as shown in fig. 3 and 4, a coupling ring 124 is further provided at the third opening 1221. It should be appreciated that, in the case where the coupling ring 124 is provided, the detachable covering member 123 may be attached to the coupling ring 124 by a felt adhesive member, such that the detachable covering member 123 is disposed over the coupling member 121 and covers the second opening 1211. For example, the felt adhesive member may be a reusable felt adhesive member such as velcro, multi-time adhesive, double-sided adhesive sheet, etc.

Continuing with fig. 5 and fig. 6, a first and a second perspective views of the cover are shown. As shown in fig. 5 and 6, in order to facilitate the attachment/detachment of the removable covering member 123, a coupling ring 124 is provided at the third opening 1221 of the filter member 122, and a recessed groove 1241 is further provided on the surface of the coupling ring 124. Furthermore, the present invention simultaneously provides an embedded ring 1231 on the bottom surface of the detachable covering member 123, and the detachable covering member 123 is attached to the coupling ring 124 by making the embedded ring 1231 embedded into the embedded groove 1241, so that the detachable covering member 123 is disposed on the coupling member 121 and covers the second opening 1211. In addition, fig. 5 and fig. 6 show that the detachable covering member 123 has a tongue piece (tab). Therefore, if the seedlings need to be separated or processed, an operator or a mechanical arm can apply a force to the tongue piece to open the removable cover 123 to expose the third opening 1221 of the filter 122, and then perform related operations through the third opening 1221.

So, the above-mentioned has completely and clearly demonstrated the cover body and the aseptic group of the box of the utility model banks up the device with earth. It should be emphasized, however, that the above detailed description is specific to possible embodiments of the invention, but the embodiments are not intended to limit the scope of the claims, and all equivalent implementations or modifications that do not depart from the technical spirit of the invention are intended to be included within the scope of the claims.

Claims

1. The utility model provides an aseptic group banks up the cover body of box with earth for thereby cover with aseptic group with the box intercombination of aseptic group bank up a first opening of box with earth, its characterized in that includes:

wherein, the detachable covering part and the filtering part are both provided with a filtering structure, thereby having the filtering function.

2. The cover of an aseptic tissue culture cassette according to claim 1, wherein the filter structure comprises:

3. The cover body of the sterile tissue culture box according to claim 1, wherein the third opening is provided with a combination ring, which is beneficial for the detachable covering piece to be attached to the combination ring through a felt adhesive piece, so that the detachable covering piece is arranged on the combination piece and covers the second opening.

4. The cover of the sterile tissue culture cassette of claim 1, wherein the third opening is provided with a coupling ring, and the surface of the coupling ring is provided with a caulking groove.

5. The cover of the sterile tissue culture cassette according to claim 4, wherein the bottom surface of the removable cover is provided with an insert ring, and the removable cover is attached to the coupling ring by inserting the insert ring into the insert groove, so that the removable cover is disposed above the coupling member and covers the second opening.

6. An aseptic tissue culture device, comprising:

a sterile tissue culture box having a first opening; and

a cover, comprising:

7. An aseptic tissue culture device according to claim 6, wherein the filter structure comprises:

8. An aseptic tissue culture device according to claim 6, wherein a coupling ring is provided at the third opening to facilitate attachment of the removable covering member to the coupling ring by a felt adhesive member, whereby the removable covering member is disposed over the coupling member and covers the second opening.

9. An aseptic tissue culture device according to claim 6, wherein a coupling ring is provided at the third opening, and a caulking groove is provided on a surface of the coupling ring.

10. An aseptic tissue culture device according to claim 9, wherein the bottom surface of the detachable covering member is provided with an embedding ring, and the detachable covering member is attached to the coupling ring by embedding the embedding ring into the embedding groove, so that the detachable covering member is disposed on the coupling member and covers the second opening.