CN217829587U - One-way flow ultrafiltration system for realizing dialysis function - Google Patents
One-way flow ultrafiltration system for realizing dialysis function Download PDFInfo
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- CN217829587U CN217829587U CN202221960023.3U CN202221960023U CN217829587U CN 217829587 U CN217829587 U CN 217829587U CN 202221960023 U CN202221960023 U CN 202221960023U CN 217829587 U CN217829587 U CN 217829587U
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Abstract
The utility model relates to a unidirectional flow ultrafiltration system for realizing dialysis function, belonging to the technical field of biological pharmacy, comprising a liquid distribution tank, an ultrafiltration tank and a unidirectional flow ultrafiltration device which are arranged in sequence; a buffer solution is arranged in the liquid preparation tank, and the buffer solution is conveyed into the ultrafiltration tank through a first liquid supply pipeline by the liquid preparation tank; a protein solution is arranged in the ultrafiltration tank, and the ultrafiltration tank conveys the protein solution to the unidirectional flow ultrafiltration device through a second liquid supply pipeline; and the unidirectional flow ultrafiltration device conveys the concentrated protein solution back to the ultrafiltration tank through a return pipeline. The solution in the ultrafiltration tank is circularly communicated with the unidirectional flow ultrafiltration device to form constant tangential flow velocity and reflux pressure so as to realize the concentration of the protein solution; under the action of the communication between the liquid preparation tank and the ultrafiltration tank, the buffer solution carries out buffer solution replacement and/or dialysis operation for removing small molecular impurities on the concentrated and filtered protein solution.
Description
Technical Field
The utility model belongs to the technical field of the bio-pharmaceuticals, specifically speaking is a realize uniflow ultrafiltration system of dialysis function.
Background
In the field of biopharmaceutical manufacturing technology, to ensure that major components in a product, such as proteins, are not denatured or inactivated by shear forces, and to maintain their original properties, a unidirectional flow ultrafiltration device (SPTFF) is used to maintain the product at a constant tangential flow rate and reflux pressure. Meanwhile, compared with a common ultrafiltration machine, the single-flow ultrafiltration has the advantages of small volume, high concentration efficiency and higher treatment efficiency on high-concentration protein products. However, in industrial use, the single-flow ultrafiltration apparatus (SPTFF) is more suitable for concentration filtration, but cannot perform dialysis for desalting a solution or replacing a solution to remove impurities.
SUMMERY OF THE UTILITY MODEL
Can't realize getting rid of the dialysis operation problem of impurity to solution in the concentrated filterable operation of bio-pharmaceuticals for the solution, the utility model provides a realize the uniflow ultrafiltration system of dialysis function.
The utility model discloses a realize through following technical scheme:
a unidirectional flow ultrafiltration system for realizing a dialysis function comprises a liquid distribution tank, an ultrafiltration tank and a unidirectional flow ultrafiltration device which are arranged in sequence;
a buffer solution is arranged in the liquid preparation tank, and the liquid preparation tank conveys the buffer solution to the ultrafiltration tank through a first liquid supply pipeline;
a protein solution is arranged in the ultrafiltration tank, and the ultrafiltration tank conveys the protein solution to the unidirectional flow ultrafiltration device through a second liquid supply pipeline; and the unidirectional flow ultrafiltration device conveys the concentrated protein solution back to the ultrafiltration tank through a return pipeline.
The solution in the ultrafiltration tank is circularly communicated with the unidirectional flow ultrafiltration device to form constant tangential flow velocity and reflux pressure so as to realize concentration of the protein solution; under the action of the communication between the liquid preparation tank and the ultrafiltration tank, the buffer solution carries out buffer solution replacement and/or dialysis operation of removing small molecular impurities on the concentrated and filtered protein solution.
The utility model discloses a further improvement still, above-mentioned join in marriage and be equipped with the weighing sensor who weighs to the buffer solution on the fluid reservoir. The output of the buffer solution is accurately controlled by a weighing sensor.
The utility model discloses a further improvement still, the aforesaid is equipped with pressure sensor on joining in marriage the fluid reservoir. The operation of the liquid preparation tank is monitored by a pressure sensor.
The utility model discloses a further improvement still, be equipped with the weighing sensor who weighs protein solution on the above-mentioned ultrafilter tank. And quantitatively acquiring the concentrated and filtered protein solution through a weighing sensor.
The utility model discloses a further improvement still is equipped with the waste discharge mouth that will filter the outside exhaust of waste liquid on the above-mentioned uniflow ultrafiltration device. The waste liquid concentrated and filtered by the unidirectional flow ultrafiltration device is discharged outwards in time through a waste discharge port.
The utility model discloses a further improvement still, the above-mentioned fluid reservoir stop motion of joining in marriage, ultrafiltration jar and uniflow ultrafiltration device circulation intercommunication. The ultrafiltration tank conveys the protein solution into the unidirectional flow ultrafiltration device for concentration and filtration, and returns the concentrated and filtered protein solution back into the ultrafiltration tank to form the primary concentration and filtration operation in the concentration and filtration process.
The utility model discloses a further improvement still, the aforesaid joins in marriage fluid reservoir, ultrafiltration jar and uniflow ultrafiltration device pipeline intercommunication and be in the running state. The ultrafiltration tank conveys the protein solution into a unidirectional flow ultrafiltration device for concentration and filtration, and returns the concentrated and filtered protein solution back to the ultrafiltration tank to form a primary concentration and filtration operation in the concentration and filtration process; the preliminarily concentrated and filtered protein solution is subjected to the buffer solution conveyed to the ultrafiltration tank from the liquid preparation tank, so that the dialysis operation of the protein solution is realized.
According to the technical scheme provided by the utility model, the beneficial effects are that: the solution in the ultrafiltration tank is circularly communicated with the unidirectional flow ultrafiltration device to form constant tangential flow velocity and reflux pressure so as to realize concentration of the protein solution; under the action of the communication between the liquid preparation tank and the ultrafiltration tank, the buffer solution carries out buffer solution replacement and/or dialysis operation for removing small molecular impurities on the concentrated and filtered protein solution.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a system diagram of an embodiment of the present invention.
In the drawings: 10. the system comprises a liquid preparation tank, 20, an ultrafiltration tank, 30, a unidirectional flow ultrafiltration device, 40, a first liquid supply pipeline, 50, a second liquid supply pipeline, 60, a return pipeline, 70 and a waste discharge port.
Detailed Description
In order to make the objects, features and advantages of the present invention more obvious and understandable, the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments, and obviously, the embodiments described below are only some embodiments, not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments in this patent without making creative efforts, shall fall within the protection scope of this patent.
As shown in the attached drawing, the unidirectional flow ultrafiltration system for realizing the dialysis function comprises a liquid preparation tank 10, an ultrafiltration tank 20 and a unidirectional flow ultrafiltration device 30 which are sequentially arranged. The unidirectional flow ultrafiltration device 30 is used for concentrating and filtering the solution in the ultrafiltration tank 20; the solution in the solution preparation tank 10 realizes the dialysis operation of desalination or solution replacement on the concentrated and filtered solution.
A buffer solution is arranged in the solution preparation tank 10, and the buffer solution is conveyed into the ultrafiltration tank 20 by the solution preparation tank 10 through a first solution supply pipeline 40;
and a weighing sensor for weighing the buffer solution is arranged on the liquid preparation tank 10. The output of the buffer solution is accurately controlled by a weighing sensor.
And a pressure sensor is arranged on the liquid distribution tank 10. The operation of the dispensing tank 10 is monitored by a pressure sensor.
A protein solution is arranged in the ultrafiltration tank 20, and the ultrafiltration tank 20 conveys the protein solution to the unidirectional flow ultrafiltration device 30 through a second liquid supply pipeline 50; the unidirectional flow ultrafiltration device 30 delivers the concentrated protein solution back into the ultrafiltration tank 20 through a return line 60.
And a weighing sensor for weighing the protein solution is arranged on the ultrafiltration tank 20. And quantitatively acquiring the concentrated and filtered protein solution through a weighing sensor.
The unidirectional flow ultrafiltration device 30 is further provided with a waste discharge port 70 for discharging the filtered waste liquid. The waste liquid concentrated and filtered by the unidirectional flow ultrafiltration device 30 is discharged outwards in time through the waste discharge port 70.
The unidirectional flow ultrafiltration device 30 is internally provided with a plurality of ultrafiltration membrane packet groups, three ultrafiltration membrane packets connected in parallel are arranged in the ultrafiltration membrane packet groups, and the ultrafiltration membrane packet groups are connected in series. Therefore, the single ultrafiltration membrane packet group has larger filtering area, sequential filtering is formed among the ultrafiltration membrane packet groups connected in series, and the filtering coefficient is high.
The liquid preparation tank 10 stops running, and the ultrafiltration tank 20 is in circulating communication with the unidirectional flow ultrafiltration device 30. The ultrafiltration tank 20 conveys the protein solution into the unidirectional flow ultrafiltration device 30 for concentration and filtration, and returns the protein solution after concentration and filtration into the ultrafiltration tank 20, thereby forming the primary concentration and filtration operation in the concentration and filtration process.
The liquid preparation tank 10, the ultrafiltration tank 20 and the unidirectional flow ultrafiltration device 30 are communicated through pipelines and are in a running state. The ultrafiltration tank 20 conveys the protein solution into the unidirectional flow ultrafiltration device 30 for concentration and filtration, and returns the concentrated and filtered protein solution back into the ultrafiltration tank 20 to form a primary concentration and filtration operation in the concentration and filtration process; the protein solution after the primary concentration and filtration is subjected to the buffer solution conveyed from the solution preparation tank 10 to the ultrafiltration tank 20, so as to realize the dialysis operation of the protein solution.
A method of operating a unidirectional flow ultrafiltration system for performing dialysis functions, comprising the steps of:
s1, liquid supply of the ultrafiltration tank 20: the ultrafiltration tank 20 conveys the protein solution to the unidirectional flow ultrafiltration device 30 through a second liquid supply pipeline 50, after concentration and filtration, the protein solution is conveyed back to the ultrafiltration tank 20 through a return pipeline 60, and the waste liquid is discharged through a waste discharge port 70; until the ultrafiltration tank 20 reaches a predetermined weight;
s2, liquid is supplied to the liquid preparation tank 10: conveying the buffer solution in the solution preparation tank 10 to the ultrafiltration tank 20 in a pressurization mode, setting the upper limit weight and the lower limit weight of the ultrafiltration tank 20, stopping conveying when the upper limit weight is reached, and continuously conveying the ultrafiltration diluent to the set upper limit weight after ultrafiltration dialysis is carried out until the weight of the ultrafiltration tank 20 reaches the set lower limit weight; repeating the steps until the set conveying total weight is reached; during the period, the unidirectional flow ultrafiltration device 30 is always in an ultrafiltration state;
and S3, repeating the step S1 until the total amount of final concentration is set.
In summary, the working principle of the system is as follows: firstly, the ultrafiltration tank 20 supplies liquid to the unidirectional flow ultrafiltration device 30, and under the action of concentration, circulation and pollution discharge of the unidirectional flow ultrafiltration device 30, the protein solution is subjected to preliminary coarse filtration for removing small molecular impurities; then the buffer solution in the tube of the liquid preparation tank 10 is input into the ultrafiltration tank 20, and the dialysis operation of desalination or solution replacement is carried out on the protein solution which is already crude in the ultrafiltration tank 20, during which the unidirectional flow ultrafiltration device 30 keeps the running state of ultrafiltration, and can filter and discharge the impurities except the protein solution after the dialysis operation; finally, the crude filtration step is repeated, and the protein solution is further concentrated and filtered to obtain a pure protein solution.
In the prior art, the unidirectional flow ultrafiltration device 30 can only perform once concentration and filtration operation on a solution, and cannot realize buffer solution replacement and/or small molecular impurity removal; if buffer solution replacement and/or small molecule impurity removal are needed, common ultrafiltration equipment is additionally equipped for realizing; the device saves the re-investment of the common ultrafiltration equipment through the system and the operation method.
The utility model discloses a unidirectional flow ultrafiltration system for realizing dialysis function, the solution in the ultrafiltration tank is circularly communicated with a unidirectional flow ultrafiltration device to form constant tangential flow velocity and reflux pressure so as to realize the concentration of protein solution; under the action of the communication between the liquid preparation tank and the ultrafiltration tank, the buffer solution carries out buffer solution replacement and/or dialysis operation of removing small molecular impurities on the concentrated and filtered protein solution.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The terms "upper", "lower", "outside", "inside" and the like in the description and claims of the present invention and the above drawings are used for distinguishing relative relationships in positions, if any, and are not necessarily given qualitatively. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the invention described herein are capable of operation in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprising" and "having," as well as any variations thereof, are intended to cover non-exclusive inclusions.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A unidirectional flow ultrafiltration system for realizing a dialysis function is characterized by comprising a liquid preparation tank (10), an ultrafiltration tank (20) and a unidirectional flow ultrafiltration device (30) which are arranged in sequence;
a buffer solution is arranged in the liquid preparation tank (10), and the liquid preparation tank (10) conveys the buffer solution into the ultrafiltration tank (20) through a first liquid supply pipeline (40);
a protein solution is arranged in the ultrafiltration tank (20), and the ultrafiltration tank (20) conveys the protein solution to the unidirectional flow ultrafiltration device (30) through a second liquid supply pipeline (50); the unidirectional flow ultrafiltration device (30) conveys the concentrated protein solution back to the ultrafiltration tank (20) through a return line (60);
and a weighing sensor for weighing the buffer solution is arranged on the liquid preparation tank (10).
2. A unidirectional flow ultrafiltration system for performing dialysis as claimed in claim 1 wherein said fluid distribution vessel (10) is provided with a pressure transducer.
3. A unidirectional flow ultrafiltration system to perform dialysis as claimed in claim 1 wherein said ultrafiltration tank (20) is provided with a load cell to weigh the protein solution.
4. The unidirectional flow ultrafiltration system for realizing dialysis function as claimed in claim 1, wherein the unidirectional flow ultrafiltration device (30) is further provided with a waste discharge port (70) for discharging filtered waste liquid outwards.
5. A unidirectional flow ultrafiltration system to achieve dialysis as claimed in any of claims 1 to 4 wherein said fluid distribution tank (10) is shut down and said ultrafiltration tank (20) is in circulating communication with a unidirectional flow ultrafiltration device (30).
6. A unidirectional flow ultrafiltration system for realizing dialysis function according to any of claims 1 to 4, wherein the fluid distribution tank (10), the ultrafiltration tank (20) and the unidirectional flow ultrafiltration device (30) are in pipeline communication and in operation.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202221960023.3U CN217829587U (en) | 2022-07-27 | 2022-07-27 | One-way flow ultrafiltration system for realizing dialysis function |
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| CN202221960023.3U CN217829587U (en) | 2022-07-27 | 2022-07-27 | One-way flow ultrafiltration system for realizing dialysis function |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115282779A (en) * | 2022-07-27 | 2022-11-04 | 山东泰邦生物制品有限公司 | Unidirectional flow ultrafiltration system for realizing dialysis function and operation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115282779A (en) * | 2022-07-27 | 2022-11-04 | 山东泰邦生物制品有限公司 | Unidirectional flow ultrafiltration system for realizing dialysis function and operation method thereof |
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