CN217819913U

CN217819913U - Flow sample processing device

Info

Publication number: CN217819913U
Application number: CN202221977425.4U
Authority: CN
Inventors: 不公告发明人
Original assignee: Hangzhou Saiji Biotechnology Co ltd
Current assignee: Hangzhou Saiji Biotechnology Co ltd
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2022-11-15
Anticipated expiration: 2032-07-28

Abstract

The utility model provides a STREAMING sample processing apparatus, include: a sample tube carrier for placing a sample tube containing a flow sample therein; the scanning unit is arranged adjacent to the sample tube carrier and used for scanning a bar code on the sample tube, recording and transmitting information of the sample tube; a processing unit for processing the streaming sample; the control unit receives the information of the sample tube transmitted by the scanning unit and controls the processing unit to perform corresponding processing on the streaming sample according to the information of the sample tube; the conveying unit is used for receiving the instruction of the control unit and conveying; and the reagent unit is used for providing reagents required by processing for the processing unit, so that the automatic processing of equipment in the flow type sample processing process is realized.

Description

Flow sample processing device

Technical Field

The utility model relates to a flow cytometry's sample preparation field especially relates to a flow type sample processing apparatus.

Background

Flow Cytometry (FCM) is a flow cytometer that is used to count and quantify various biological and physical and biochemical characteristics of blood, various body fluids, bone marrow, biopsy materials, single cell suspensions of animals and plants, paraffin-embedded tissues, including cells, platelets, organelles, sperm, microorganisms, and artificially synthesized microspheres, etc.

Before the flow analysis detection is carried out, necessary processing and preparation must be carried out on a sample to be detected, wherein the quality of the preparation of the sample to be detected directly influences the effectiveness and accuracy of data detection.

At present, a sample to be measured of a flow analyzer is usually processed by a human before measurement, and the preparation process is very complicated. During manual processing, the sample is exposed in the air, and particularly during uniform mixing, the sample can splash due to liquid level oscillation, so that high biological risk is realized. Manual handling of samples is subject to experience and handling habits of the handler and often results in inaccurate test results.

Therefore, there is a need to develop a new flow sample processing device to improve some of the above problems in the prior art.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a STREAMING sample processing apparatus can realize the automated processing sample.

In order to achieve the above object, the present invention provides a flow type sample processing device, including: a sample tube carrier for placing a sample tube containing a streaming sample therein; the scanning unit is arranged adjacent to the sample tube carrier and is used for scanning a bar code on the sample tube, recording and transmitting the information of the sample tube; a processing unit for processing the streaming sample; the control unit receives the information of the sample tube transmitted by the scanning unit and controls the processing unit to perform corresponding processing on the streaming sample according to the information of the sample tube; the conveying unit is used for receiving the instruction of the control unit and conveying; and the reagent unit is used for providing reagents required by treatment to the treatment unit.

The utility model provides a STREAMING sample processing apparatus's beneficial effect lies in: the scanning unit scans and records the sample tube in the sample tube carrier, the recorded information of the sample tube is transmitted to the control unit, the control unit controls the processing unit to perform corresponding processing on the sample according to different information of the sample tube, the carrying unit performs carrying operation on a target to be carried, the reagent unit provides reagents required for processing, automatic processing of equipment in a flow type sample processing process is achieved, manual operation is not needed, accuracy of flow type sample processing operation and quality of a flow type sample processing result are improved, a flow type sample processing procedure is completed in the flow type sample processing device, risk of biological infection is reduced, and personal safety of operators is improved.

Optionally, the sample tube carrier comprises a slide extending in a first direction for sliding the sample tube into the sample tube carrier in the first direction. The beneficial effects are that: the convenience of placing the sample tube is improved.

Optionally, the slide way includes an empty section and a placing section arranged along a first direction, and the sample tube enters the placing section after passing through the empty section; the scanning position of the scanning unit is positioned in the vacant section. The beneficial effects are that: the sample tube carrier is provided with a plurality of rows of slide ways for placing the sample tubes, when the sample tubes pass through the vacant section, the bar codes on the sample tubes can be scanned by the scanning unit, and after the scanning is finished, the sample tubes enter the placement section, so that the sample tubes cannot exist in the vacant section; when the sample tubes in the rear row enter the slide ways in the rear row, the sample tubes can be scanned in the corresponding vacant sections, and the vacant sections in the slide ways in the front row are not provided with the sample tubes, so that the scanning of the scanning unit is not blocked.

Optionally, the sample tube carrier comprises a tube to be processed carrier and a processed tube carrier, the tube to be processed carrier being arranged in parallel with the processed tube carrier, the tube to be processed carrier being located between the scanning unit and the processed tube carrier. The beneficial effects are that: the sample tubes to be processed and processed are placed in a centralized mode, convenience in operation is improved, meanwhile, the scanning unit can also scan the processed sample tubes, information of processing completion is fed back to the control unit, and automatic processing is achieved.

Optionally, the processing unit includes a centrifuge and a balancing plate, the centrifuge is used for centrifuging the sample, and the balancing plate is arranged adjacent to the centrifuge. The beneficial effects are that: the automation of the centrifugal processing operation in the flow type sample processing process is realized.

Optionally, the processing unit comprises an oscillation incubator and a light shielding plate, and the light shielding plate is arranged on the sample tube carrier. The beneficial effects are that: realize shaking the mixing, simultaneously the light screen provides the condition of light-resistant hatching.

Optionally, a heating module is arranged in the shaking incubator. The beneficial effects are that: the heating module controls the incubation temperature within a target range to realize constant-temperature incubation.

Optionally, a mixer is arranged in the reagent unit.

Optionally, the flow-type sample processing device further includes a sample application unit for applying the reagent in the reagent unit to a target position, and the sample application unit includes at least four sample application channels.

Drawings

Fig. 1 is a schematic structural diagram of a flow sample processing device according to an embodiment of the present invention.

Reference numerals are as follows:

101. placing the sections; 102. a vacant section; 2. a barcode scanner; 3. a needle magazine location; 4. a distilled water tank; 5. Needle removing position; 6. a waste liquid tank; 7. a dilution position; 8. a reagent bottle position; 9. a hemolytic agent site; 10. phosphate buffered saline reagent site; 11. oscillating the incubation position; 12. a first matching plate; 13. a centrifuge; 14. a second matching plate; 15. sample adding position; 16. a visor; 17. an operation panel 17; 1001. a pipe carrier to be processed; 1002. And (4) carrying the treated pipe.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without making creative efforts belong to the protection scope of the present invention. Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention belongs. As used herein, the word "comprising" and similar words are intended to mean that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items.

For solving the problem that prior art exists, the embodiment of the utility model provides a STREAMING sample processing apparatus.

In some embodiments of the present invention, the flow sample processing device comprises: a sample tube carrier for placing a sample tube containing a flow sample therein; the scanning unit is arranged adjacent to the sample tube carrier and used for scanning a bar code on the sample tube, recording and transmitting information of the sample tube; a processing unit for processing the streaming sample; the control unit receives the information of the sample tube transmitted by the scanning unit, executes different detection items according to the information of the sample tube, starts a preset program of an instrument, and controls the processing unit to perform corresponding processing on the streaming sample according to the information of the sample tube; the conveying unit is used for receiving the instruction of the control unit and conveying; and the reagent unit is used for providing reagents required by the treatment to the treatment unit.

Fig. 1 is a schematic structural diagram of the flow sample processing device according to an embodiment of the present invention.

In some embodiments, referring to fig. 1, the flow sample processing device includes an operation panel 17, and the operation panel 17 is used for placing each unit of the device.

In some embodiments of the present invention, the scanning unit includes a barcode scanner 2, which decodes the barcode content and transmits the decoded barcode content to the control unit in a wireless or wired manner; the bar code scanner 2 is arranged at the left end of the operation panel 17, and the scanning direction of the bar code scanner is vertical to the arrangement direction of the sample tube carrier.

In some embodiments, the carrying unit includes a carrying hand, which has the freedom degrees in the length direction translation, the width direction translation and the three mutually perpendicular directions close to the operation panel 17 along the operation panel 17, and is used for carrying the parts in the flow sample processing device; for example, the deep well plate is transported to the centrifuge 13 and centrifuged.

In some embodiments of the present invention, referring to fig. 1, the sample tube carrier comprises a pending tube carrier 1001 and a processed tube carrier 1002, the pending tube carrier 1001 is disposed between the barcode scanner 2 and the processed tube carrier 1002; the sample tube carrier is arranged in the vertical direction of the figure and comprises a plurality of slideways.

In some embodiments of the present invention, referring to fig. 1, the deep hole plate is a 96 deep hole plate.

In some embodiments of the present invention, referring to fig. 1, the pending tube carrier 1001 and the processed tube carrier 1002 all include six rows of slides, which are arranged along the vertical direction of the drawing.

In some embodiments of the present invention, referring to fig. 1, the slide includes an empty section 102 and a placing section 101 arranged along a first direction, the sample tube is pushed into the slide from the lower end of the slide, and the sample tube finally enters the placing section 101 to be placed after passing through the empty section 102; the barcode scanner 2 is disposed on the left side of the illustration of the vacant section 102, and scans barcodes on the sample tubes in the sample tube carrier entering the vacant section 102 one by one.

In some embodiments of the present invention, referring to fig. 1, the operation panel 17 is further provided with four needle box locations 3, the needle box locations 3 are located on the right side of the sample tube support, any one of the needle box locations 3 can place 96 suction heads.

In some embodiments of the present invention, referring to fig. 1, the operation panel 17 is further provided with a distilled water tank 4, and the distilled water tank 4 is located below the right side of the diagram of the needle box 3 for balancing before centrifugation.

In some embodiments of the present invention, the operation panel 17 is further provided with a needle-off position 5, the needle-off position 5 is located on the right side of the diagram of the distilled water tank 4, and the sample adding unit moves to the place where the waste suction head is discarded.

In some embodiments of the present invention, referring to fig. 1, the operation panel 17 is further provided with a waste liquid tank 6, the waste liquid tank 6 is located on the right side of the diagram of the needle-off position 5, and the sample adding unit moves to the place to discard waste liquid.

In some embodiments of the present invention, referring to fig. 1, the reagent unit includes a dilution position 7, the dilution position includes 16 placing holes, and the multistage gradient dilution of the standard is realized.

In some embodiments of the present invention, referring to fig. 1, the reagent unit comprises a hemolytic agent position 9 for holding hemolytic agent for performing hemolytic treatment on a flow type sample requiring hemolysis.

In some embodiments of the present invention, referring to fig. 1, the reagent unit includes Phosphate Buffered Saline (PBS) reagent level 10 for holding PBS reagent.

In some embodiments of the present invention, referring to fig. 1, the processing unit includes a centrifuge 13 and a matching plate, the centrifuge 13 is used for centrifuging a reagent and a sample to which the reagent is added, and the matching plate is disposed adjacent to the centrifuge 13; the trim plate includes a first trim plate 12 and a second trim plate 14, the first trim plate 12 for capture microsphere trim and the second trim plate 14 for deep well plate trim.

The utility model discloses some embodiments, refer to fig. 1, the processing unit is including vibrating and is hatched position 11 and light screen 16, vibrate and hatch and be equipped with the vibration and hatch the ware in position 11, light screen 16 set up in on the sample pipe carrier frame.

The utility model discloses in some embodiments, vibrate and be provided with heating module in the incubator, realize that constant temperature is hatched.

The utility model discloses in some embodiments, refer to fig. 1, the reagent unit includes two

reagent bottle positions

8, 8 below in reagent bottle position are provided with the blending machine, carry out eccentric rotation type mixing to reaction liquid before the operation.

In some embodiments of the present invention, the reagent position with swing joint between the operating panel 17, promptly but the handle of reagent position is proposed, but the shelf of placing reagent in the reagent position is manual also to satisfy the different demands when actually carrying out STREAMING processing.

The utility model discloses in some embodiments, refer to fig. 1, still be provided with application of sample position 15 on the operating panel 17, the application of sample unit absorbs remove behind the sample in the sample tube extremely application of sample position 15, it is right the corresponding hole of the deep hole board in application of sample position 15 carries out the application of sample.

The utility model discloses some embodiments, STREAMING sample processing apparatus still includes the application of sample unit, be used for with in the reagent unit reagent application of sample to target location, the application of sample unit includes four at least application of sample passageways.

The utility model discloses in some embodiments, the processing unit basis bar code information on the sample tube is right flow type sample handles, include: and (3) uniformly mixing the microspheres, centrifuging, removing supernatant, adding diluent, shaking and uniformly mixing, and incubating in a dark place.

The utility model discloses in some embodiments, the processing unit basis bar code information on the sample tube is right flow type sample handles, include: and (4) diluting, uniformly mixing and diluting the standard product in a gradient manner.

The utility model discloses in some embodiments, the processing unit basis bar code information on the sample tube is right flow type sample handles, include: after adding the sample, the standard, the microspheres and the fluorescent agent, automatically shaking and uniformly mixing and incubating in a dark place.

The processing unit processes the streaming sample according to the barcode information on the sample tube, and comprises: after incubation, centrifugation, supernatant removal and PBS resuspension were added, and the samples were transferred.

Although the embodiments of the present invention have been described in detail hereinabove, it is apparent to those skilled in the art that various modifications and variations can be made to these embodiments. However, it is to be understood that such modifications and variations are within the scope and spirit of the present invention as set forth in the appended claims. Moreover, the invention as described herein is capable of other embodiments and of being practiced or of being carried out in various ways.

Claims

1. A streaming sample processing device, comprising:

a sample tube carrier for placing a sample tube containing a streaming sample therein;

the scanning unit is arranged adjacent to the sample tube carrier and used for scanning a bar code on the sample tube, recording and transmitting information of the sample tube;

a processing unit for processing the streaming sample;

the control unit receives the information of the sample tube transmitted by the scanning unit and controls the processing unit to perform corresponding processing on the streaming sample according to the information of the sample tube;

the conveying unit is used for receiving the instruction of the control unit and conveying;

and the reagent unit is used for providing reagents required by treatment to the treatment unit.

2. The streaming sample processing device of claim 1, wherein the sample tube carrier includes a slide extending in a first direction for sliding the sample tube into the sample tube carrier in the first direction.

3. The streaming sample processing device of claim 2, wherein the slide includes a vacant section and a placement section aligned in a first direction, the sample tube passing through the vacant section and into the placement section; the scanning position of the scanning unit is positioned in the vacant section.

4. The flow sample processing device according to claim 3, wherein the sample tube carrier comprises a tube-to-be-processed carrier and a tube-after-processing carrier, the tube-to-be-processed carrier and the tube-after-processing carrier being disposed in parallel, the tube-to-be-processed carrier being located between the scanning unit and the tube-after-processing carrier.

5. The flow-type sample processing device according to claim 1, wherein the processing unit comprises a centrifuge for centrifuging a sample and a balancing plate disposed adjacent to the centrifuge.

6. The flow sample processing device of claim 1, wherein the processing unit comprises an oscillating incubator and a light baffle disposed on the sample tube carrier.

7. The flow sample processing device according to claim 6, wherein a heating module is disposed within the shaking incubator.

8. The flow-type sample processing device according to claim 1, wherein a mixer is provided in the reagent unit.

9. The streaming sample processing device according to claim 8, further comprising a loading unit for loading the reagent in the reagent unit to a target location, the loading unit comprising at least four loading channels.