CN217809468U

CN217809468U - Temperature control device of PCR amplification instrument

Info

Publication number: CN217809468U
Application number: CN202221952288.9U
Authority: CN
Inventors: 付文成; 纪文威; 李思斌; 孙相鑫
Original assignee: Yihong Shenzhen Gene Co ltd
Current assignee: Yihong Shenzhen Gene Co ltd
Priority date: 2022-07-27
Filing date: 2022-07-27
Publication date: 2022-11-15
Anticipated expiration: 2032-07-27

Abstract

A temperature control device of a PCR amplification instrument comprises: the heating mechanism, the refrigerating mechanism, the blending mechanism and the air bath chamber are arranged in the air bath chamber; the heating mechanism includes: the heating and drainage fan, the heating air duct, the heating assembly and the heating temperature control part; the refrigeration mechanism includes: the refrigeration drainage fan, the refrigeration air duct and the refrigeration assembly; the air outlet of the refrigeration air duct is communicated with the heating air duct, and the refrigeration drainage fan and the refrigeration assembly are arranged inside the refrigeration air duct; the mixing mechanism includes: the mixing device comprises a mixing chamber and a mixing assembly, wherein the mixing chamber is provided with a mixing air inlet and a mixing air outlet, the mixing assembly is arranged inside the mixing chamber, and the mixing air inlet is communicated with an air outlet of a heating air duct; the air bath air inlet is communicated with the uniformly mixing air outlet; the heating drainage fan is used for guiding airflow to flow through the heating assembly, the refrigerating drainage fan is used for guiding airflow to flow through the refrigerating assembly, the blending assembly is used for blending airflow, and the air bath air outlet is used for exhausting airflow. The temperature required by sample detection can be rapidly obtained by heating the airflow, so that the detection efficiency is improved.

Description

Temperature control device of PCR amplification instrument

Technical Field

The application relates to the technical field of biomedical detection, in particular to a temperature control device of a PCR amplification instrument.

Background

The Polymerase Chain Reaction (PCR) is a molecular biology technique for amplifying and amplifying specific DNA fragments, and the advent of PCR amplification equipment has enabled the automation of PCR technology and the wide application of PCR technology in such areas as genetic disease diagnosis, nucleic acid detection of pathogens in clinical specimens, genetic identification, gene replication, and analysis of mutations in activated oncogenes.

The traditional PCR reaction has long detection time, and the increase of the detection efficiency is still restricted by the false negative and false positive events which occur from time to time. The accuracy of PCR detection is influenced by the design of primers, reaction reagents and a reaction system, and the uniformity of reaction temperature control is also important. Similarly, a temperature control system with a faster response speed can help to reduce nucleic acid detection time.

The existing heating modes for the PCR amplification instrument can be divided into a semiconductor type and a hot air heating type, and the semiconductor type PCR amplification instrument is relatively early in origin and relatively mature in development. The semiconductor cooler (TEC) can refrigerate and heat, and is a scheme adopted by most manufacturers, but the defects of the semiconductor cooler (TEC) are very obvious, firstly, the temperature rise of the TEC is slow, and the temperature rise rate is only 2-3 ℃/s, so that the amplification period of the PCR amplification instrument is greatly prolonged. Secondly, the TEC heating mostly adopts hard contact heating, metal is used for conducting heat, and the requirement on a carrier is high in order to enable the temperature of a target object to be changed rapidly. The hot air type PCR amplification instrument has the advantages of low cost, high temperature rise and fall response speed, high compatibility of the PCR amplification instrument carrier and the like. But hot-blast type PCR amplificator on the one hand temperature is difficult to accurate control, and temperature homogeneity is general relatively poor, and on the other hand, hot-blast type PCR amplificator is through reducing heating power's mode when needing the cooling, and cooling speed is low, and then influences detection efficiency.

SUMMERY OF THE UTILITY MODEL

The application provides a temperature control device of PCR amplification instrument that can go up and down temperature fast.

The application provides a temperature control device of PCR amplificator, includes: the heating mechanism, the refrigerating mechanism, the blending mechanism and the air bath chamber are arranged in the air bath chamber; the heating mechanism includes: the heating and drainage fan, the heating air duct, the heating assembly and the heating temperature control part; the refrigeration mechanism includes: the refrigeration drainage fan, the refrigeration air duct and the refrigeration assembly; the heating drainage fan and the heating assembly are both arranged inside the heating air channel; the heating temperature control is connected with the heating assembly, the heating assembly is used for generating heat, and the heating temperature control is used for controlling the temperature generated by the heating assembly; the air outlet of the refrigerating air duct is communicated with the heating air duct, and the refrigerating drainage fan and the refrigerating assembly are arranged inside the refrigerating air duct; the blending mechanism comprises: the mixing device comprises a mixing chamber and a mixing component, wherein the mixing chamber is provided with a mixing air inlet and a mixing air outlet, the mixing component is arranged inside the mixing chamber, and the mixing air inlet is communicated with the air outlet of the heating air duct; the air bath chamber is used for placing a sample to be amplified, and is also provided with a first visual window for observing the sample to be amplified; the air bath chamber is provided with an air bath air inlet and an air bath air outlet, and the air bath air inlet is communicated with the uniformly mixing air outlet; the heating drainage fan is used for guiding airflow to flow through the heating assembly, the refrigeration drainage fan is used for guiding airflow to flow through the refrigeration assembly, the blending assembly is used for blending airflow, and the air bath air outlet is used for exhausting airflow.

In one embodiment, the method further comprises: the air bath device comprises a square-shaped guide air duct, a water bath cavity and a water tank, wherein the square-shaped guide air duct is provided with an inner shell and an outer shell sleeved on the inner shell; the outer shell is provided with a flow guide air inlet, the inner shell is provided with a flow guide air outlet, the flow guide air inlet is communicated with the air outlet of the heating air duct, and the flow guide air outlet is communicated with the air bath air inlet of the air bath cavity; the clip-shaped guide air duct is also provided with a second window which is just opposite to the first window, and the second window penetrates through the inner shell and the outer shell.

In one embodiment, the refrigeration mechanism further comprises: the air outlet in refrigeration wind channel with the intercommunication phase in heating wind channel is relative to the position that heating element set up is close to in heating wind channel's air outlet, prevent that the subassembly that flows backwards is used for preventing that heating wind channel's backward flow air current from entering into the refrigeration wind channel.

In one embodiment, the refrigeration mechanism further comprises: the backflow assembly is used for enabling backflow airflow to flow back to the heating air duct; the backflow component is close to the air outlet of the heating air duct relative to the backflow-preventing component.

In one embodiment, the backflow prevention assembly comprises: the backflow prevention guide part comprises a backflow prevention guide part, a first backflow prevention part and a second backflow prevention part; the backflow prevention flow guide part is used for guiding backflow airflow to the first backflow prevention part, and the first backflow prevention part is used for enabling the backflow airflow guided to the first backflow prevention part to turn back to the second backflow prevention part and enabling the backflow airflow to be discharged to the heating air duct.

In one embodiment, the reflow assembly includes: a backflow guide part, a first backflow part and a second backflow part; the backflow guide part is used for guiding backflow airflow to the first backflow part, the first backflow part is used for enabling the backflow airflow guided to the first backflow part to return to the second backflow part, and the second backflow part is used for enabling the backflow airflow to be discharged from an air outlet of the refrigeration air duct.

In one embodiment, the blending assembly comprises: the blending driving unit is used for driving the blending rotating shaft to rotate so as to drive the blending fan blades to rotate; the axial lead of the uniformly mixing rotating shaft is coincided with the central axis of the heating air duct; the blending fan blade is provided with a plurality of hollow parts.

In one embodiment, the air bath cavity is internally provided with a temperature measuring module, and the temperature measuring module is connected with the heating temperature control part and the refrigerating drainage fan and is used for detecting temperature information in the air bath cavity and outputting the temperature information to the heating temperature control part and the refrigerating drainage fan.

In one embodiment, the air bath air outlet is further provided with an air bath air outlet fan.

In one embodiment, the air inlet of the air bath is arranged at the bottom end of the air bath chamber, and the air outlet of the air bath is arranged at the top end or the side surface of the air bath chamber.

According to the temperature control device of the PCR amplification instrument of the embodiment, the heating and drainage fan can guide airflow to flow through the heating assembly, the airflow is guided to flow through the mixing chamber from the air outlet of the heating channel and is guided to the air bath chamber from the air bath air inlet, the heating assembly heats the airflow to form heating airflow, and the heating temperature of the heating assembly can be controlled through the heating temperature control element, so that the heating temperature meets the temperature required by sample detection. When the actual temperature in the air bath chamber is higher than the temperature required by detection, the heating temperature generated by the heating assembly is controlled by the heating temperature control. When the difference between the actual temperature in the air bath cavity and the temperature required for detection is great, the refrigeration drainage fan can guide the air current to flow through the refrigeration assembly, and guide to the heating air duct from the air outlet of refrigeration passageway, form the refrigeration air current to the air current refrigeration through the refrigeration assembly, the mixing subassembly can carry out the mixing with heating air current and mixing air current, the air current of mixing satisfies the temperature required for detection, and flow into the air bath cavity through the air bath air intake. In the above processes of adjusting the air temperature, the air outlet of the air bath can discharge the air flow flowing into the air bath chamber, so that the air bath chamber can continuously supplement the heating air flow, the circularly flowing heating air flow is formed, and the uniformity of the heating temperature is ensured. The temperature required by sample detection can be quickly obtained by heating the air flow, so that the detection efficiency is improved.

Drawings

FIG. 1 is a perspective view of a temperature control device of a PCR amplification apparatus provided in the present application;

FIG. 2 is a sectional view of a temperature control device of the PCR amplification apparatus provided in the present application;

FIG. 3 is an enlarged view of a portion of FIG. 2 at A;

FIG. 4 is a cross-sectional view of another perspective of the temperature control device of the PCR amplification apparatus provided in the present application;

FIG. 5 is a schematic block diagram illustrating the temperature control of the temperature control device of the PCR amplification apparatus provided herein;

FIG. 6 is a schematic structural diagram of a mixing component in a temperature control device of the PCR amplification apparatus provided in the present application.

Detailed Description

The present application will be described in further detail below with reference to the accompanying drawings by way of specific embodiments. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments, and the operation steps involved in the embodiments may be interchanged or modified in order as will be apparent to those skilled in the art. Accordingly, the description and drawings are merely for clarity of description of certain embodiments and are not intended to necessarily constitute and/or sequence.

The ordinal numbers used herein for the components, such as "first," "second," etc., are used merely to distinguish between the objects described, and do not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The application provides a temperature control device of PCR amplification instrument, is mainly applied to the PCR amplification instrument and treats the amplification sample and heat, wherein, treats the sample of amplification for the sample of storing in the test tube, and the sample can be saliva, the pharyngeal liquid etc. that mix with the reagent. The test tube is typically housed in a wind bath chamber and heated by means of heated air. Of course, in other embodiments, other products to be heated may be received in the air bath chamber.

This embodiment provides a temperature control device of a PCR amplification apparatus, referring to fig. 1-5, the temperature control device of the PCR amplification apparatus includes: a heating mechanism 10, a refrigerating mechanism 20, a blending mechanism 30 and an air bath chamber 40.

The heating mechanism 10 includes: the heating and drainage fan 11, the heating air duct 12, the heating component 13 and the heating temperature control piece 14. The refrigeration mechanism 20 includes: a refrigeration drainage fan 21, a refrigeration air duct 22 and a refrigeration assembly 23; heating drainage fan 11 and heating element 13 all locate the inside of heating wind channel 12, and heating temperature control spare 14 is connected with heating element 13, and heating element 13 is used for producing heat, and heating temperature control spare 14 is used for controlling the temperature of the heat that heating element 13 produced. The heating air duct 12 and the cooling air duct 22 both have an air inlet and an air outlet, and for the sake of convenience of distinction, the air inlet and the air outlet of the heating air duct 12 are respectively referred to as a heating air inlet 121 and a heating air outlet 122, and the air inlet and the air outlet of the cooling air duct 22 are respectively referred to as a cooling air inlet 221 and a cooling air outlet 222. The refrigerating air outlet 222 of the refrigerating air duct 22 is communicated with the heating air duct 12, the heating air inlet 121 and the refrigerating air inlet 221 are both communicated with the outside atmosphere, and the refrigerating drainage fan 21 and the refrigerating assembly 23 are both arranged inside the refrigerating air duct 22. The kneading mechanism 30 includes: the mixing device comprises a mixing chamber 31 and a mixing assembly 32, wherein the mixing chamber 31 is provided with a mixing air inlet 311 and a mixing air outlet 312, the mixing assembly 32 is arranged inside the mixing chamber 31, and the mixing air inlet 311 of the mixing chamber 31 is communicated with a heating air outlet 122 of the heating air duct 12. The air bath chamber 40 is used for placing a sample to be amplified, the air bath chamber 40 is provided with an air bath air inlet 41 and an air bath air outlet 42, and the air bath air inlet 41 is communicated with the uniform mixing air outlet 312. Heating drainage fan 11 is used for guiding the air current to flow through heating element 13, heat the air current of flowing through heating element 13, in order to form the heated air current, refrigeration drainage fan 21 is used for guiding the air current to flow through refrigeration subassembly 23, refrigerate the air current of flowing through refrigeration subassembly 23, in order to form the refrigerated air current, mixing subassembly 32 is used for the mixing air current, it is specific that mixing heated air current and system cold air flow down, in order to form the mixing air current, the mixing air current enters into the inside of wind bath cavity 40 through wind bath air intake 41, in order to treat the sample that augmentates and heat. The air bath outlet 42 is used for discharging air flow, specifically, discharging uniform air flow entering the air bath chamber 40, so that the inside of the air bath chamber 40 can always keep the air flow flowing state, and the uniformity of the heating temperature is ensured.

In some embodiments, an air bath outlet fan is further disposed at the air bath outlet 42 to improve the air flow discharging efficiency.

In this embodiment, heating element adopts the mode of heater strip heating to heat, and refrigeration component adopts the mode of semiconductor refrigeration piece to refrigerate.

As shown by the dotted arrows in fig. 2, the flow directions of the heating air flow and the cooling air flow are shown by the dotted arrows. The specific working process of the temperature control device of the PCR amplification apparatus provided in this embodiment is as follows: the heating and drainage fan 11 works to guide the air flow to enter the inside of the heating air duct 12 from the outside through the heating air inlet 121 and flow through the heating assembly 13, the air flow is heated through the heating assembly 13 to form a heating air flow, and the heating air flow enters the inside of the air bath chamber 40 through the heating air outlet 122, the uniformly mixing air inlet 311, the uniformly mixing chamber 31, the uniformly mixing air outlet 311 and the air bath air inlet 41 under the action of the heating and drainage fan 11 to heat the sample to be amplified. The heating temperature control 14 controls the temperature of the heat generated by the heating assembly to control the heating temperature of the heating airflow, so that the heating temperature reaches a preset temperature required for detecting the sample to be amplified.

In the above process, the refrigeration assembly 23 may not work or work, and performs refrigeration in a working state, and may rapidly refrigerate the external air flow guided from the refrigeration air inlet 221 by the refrigeration guide fan 21. In this embodiment, when the heating assembly 13 heats the airflow flowing through the heating air duct 12, the cooling assembly 23 simultaneously cools the airflow flowing through the cooling air duct 22, so as to obtain the cooling airflow rapidly.

In one embodiment, when the temperature of the heating airflow is greater than the preset temperature and the difference between the two is small, the temperature of the heating airflow can be adjusted to the preset temperature by adjusting the temperature of the heat generated by the heating assembly 13 through the heating temperature control 14.

In another embodiment, when the temperature of the heated air flow is greater than the preset temperature and the difference between the two temperatures is large, or when the temperature of the heated air flow needs to be rapidly reduced, the cooling drainage fan 21 works to guide the air flow to enter the inside of the cooling air duct 22 through the cooling air inlet 221 from the outside and flow through the cooling component 23, because the cooling component is in a working state, the cooling air flow can be rapidly obtained, the cooling air flow passes through the cooling air outlet 222, the uniform mixing air inlet 311 and the uniform mixing chamber 31 under the action of the cooling drainage fan 21, and is uniformly mixed in the uniform mixing chamber 31 under the action of the uniform mixing component 32 to form a uniform mixing air flow, the temperature of the uniform mixing air flow meets the preset temperature, and the uniform mixing air flow enters the inside of the air bath chamber 40 through the uniform mixing air outlet 311 and the air bath air inlet 41, so as to heat the sample to be amplified.

In this embodiment, the sample to be amplified inside the air bath chamber is heated by heating the air flow, so that the sample to be amplified can quickly reach the preset temperature, and the detection efficiency of the sample to be amplified is further improved. When the temperature of the heating airflow is higher than the preset temperature and the temperature difference between the heating airflow and the preset temperature is large, the uniform mixing airflow reaching the preset temperature can be quickly obtained through the refrigerating airflow, and the detection efficiency is further improved.

In this embodiment, the air bath cavity 40 is further provided with a temperature measurement module 43 inside, the temperature measurement module 43 is connected with the heating temperature control 14 and the refrigeration drainage fan 21, and the temperature measurement module 43 is used for detecting temperature information in the air bath cavity 40 and outputting the temperature information to the heating temperature control 14 and the refrigeration drainage fan 21. In a preferred embodiment, the thermometry module 43 may be a temperature sensor.

Specifically, when the temperature measuring module 43 detects that the temperature in the air bath chamber 40 is the preset temperature, the heating temperature control 14 may control the heating assembly 13 to maintain the current heating state. When the temperature measuring module 43 detects that the temperature in the air bath cavity 40 is higher than the preset temperature and the difference between the two is small, the heating temperature control 14 can control the heating component 13 to increase the heating power so as to increase the heating temperature until the temperature measuring module 43 detects that the temperature in the air bath cavity 40 is the preset temperature. When temperature measuring module 43 detects that the temperature in the air bath cavity 40 is greater than preset temperature, and the difference between the two is great, then control refrigeration drainage fan 21 work, flow refrigeration air current to mixing chamber and carry out the mixing through the mixing subassembly to form the mixing air current, the mixing air current flows into the inside of air bath cavity 40 again, and the temperature in the air bath cavity 40 that detects is up to temperature measuring module 43 reaches preset temperature.

In one embodiment, the air bath chamber 40 is further provided with a first viewing window for observing the sample to be amplified, and the first viewing window is made of a transparent material. The reaction process of the sample to be amplified can be photographed through the first visual window. In another embodiment, the air bath chamber 40 may be made of a transparent material.

In an embodiment, for guaranteeing that heating air current and refrigeration air current can further mix under the effect of mixing subassembly, the further mixing of accessible extension mixing air current's flow path's mode, so, the temperature control device of the PCR amplificator that this embodiment provided still includes: the air bath device comprises a return type guide air duct 50, wherein the return type guide air duct 50 is provided with an inner shell 54 and an outer shell 55 sleeved on the inner shell 54, an air bath cavity 40 is arranged at the inner ring of the inner shell 54, the outer shell 55 is provided with a guide air inlet 51, the inner shell 54 is provided with a guide air outlet 52, the guide air inlet 51 is communicated with a heating air outlet 122 of the heating air duct 12, and the guide air outlet 52 is communicated with an air bath air inlet 41 of the air bath cavity 40. Through the setting in the type water conservancy diversion wind channel that returns for the air current that flows out from the heating air outlet drainage in heating wind channel divides into two strands, and further flows a section distance, in order to reach the effect of abundant mixing.

In this embodiment, the circular air guiding duct 50 is further provided with a second window opposite to the first window, and the second window is also made of a transparent material.

In one embodiment, it can be considered that the inner shell 54 is a shell structure forming the air bath chamber 40, so that the first window is the air bath inlet 41 of the air bath chamber 40, and the first window may not be provided, and only the second window is provided on the outer shell 55, as shown in fig. 4, and the mounting hole 551 for mounting the second window is provided on the outer shell 55.

In this embodiment, be provided with two refrigeration mechanism, two refrigeration mechanism are symmetrical about heating mechanism, can provide the refrigeration air current of more amount of wind to reach quick cryogenic effect.

In one embodiment, both the cooling mechanism and the heating mechanism are disposed above the air bath chamber, and the air bath chamber 40 is disposed inside the return-type guiding air duct 50. In other words, both the refrigerating mechanism and the heating mechanism are disposed above the return-type air guide duct 50.

In this embodiment, the sample to be amplified is preferably heated from the bottom, so that the air inlet 41 of the air bath is disposed at the bottom of the air bath chamber 40, the air outlet 42 of the air bath is disposed at the top or side of the air bath chamber 40, and preferably, the air outlet 42 of the air bath is disposed at the side of the air bath chamber 40.

In one embodiment, in order to facilitate the observation of the detection process of the sample to be amplified, a viewing window is further disposed on the side wall of the wind guide duct 50 opposite to the wind inlet 41 of the wind bath, and the viewing window may be made of a transparent material.

As shown in fig. 1, a sample inlet 53 is further opened on a side wall of the rectangular diversion duct 50, and the sample inlet 53 is communicated with the inside of the air bath chamber 40 so as to put the sample to be amplified into the air bath chamber 40.

Referring to FIG. 6, the blending assembly 32 includes: the blending driving unit (not shown in the figure), the blending rotating shaft 321 and the blending fan blades 322, each blending fan blade 322 is radially installed on the blending rotating shaft 321, the blending rotating shaft 321 is in transmission connection with the blending driving unit, and the blending driving unit is used for driving the blending rotating shaft 321 to rotate so as to drive each blending fan blade 322 to rotate. In this embodiment, the axial lead of the mixing rotating shaft 321 coincides with the central axis of the heating air duct 12, and the mixing fan blades 322 are provided with a plurality of hollow portions 323, so that the rotating mixing fan blades 322 can form a turbulent flow effect due to the arrangement of the hollow portions 323, and can better mix the heating air flow and the cooling air flow.

When the mixing fan blades 322 mix the heated air flow and the cooled air flow, a part of the air flow reversely flows due to the turbulent flow, so as to form a backflow air flow, and the backflow air flow flowing reversely flows back to the inside of the cooling air duct 22. As shown in fig. 3, the refrigeration mechanism 20 further includes: the communication position of the refrigeration air outlet 222 of the refrigeration air duct 22 and the heating air duct 12 of the backflow-preventing component 23 is close to the heating air outlet 122 of the heating air duct 12 relative to the position where the heating component 12 is arranged, and the backflow-preventing component 23 is used for preventing backflow air of the heating air duct 12 from entering the refrigeration air duct 22.

Specifically, the backflow prevention assembly 23 includes: a backflow prevention guide part 231, a first backflow prevention part 232 and a second backflow prevention part 233; the backflow prevention guide part 231 is used for guiding backflow airflow to the first backflow prevention part 232, the first backflow prevention part 232 is used for enabling backflow airflow guided to the first backflow prevention part 232 to return to the second backflow prevention part 233, and the backflow airflow is discharged from the refrigeration air outlet 222 of the refrigeration air duct 22 under the action of the second backflow prevention part 233. Fig. 3 shows the flow direction of the backflow prevention assembly, which can return most of the backflow air to the heating air duct 12.

In this embodiment, the refrigeration mechanism 20 further includes: the backflow component 24 and the backflow component 24 are disposed between the backflow-preventing component 23 and the heating air outlet 122 of the heating air duct 12, the backflow component 24 is used for enabling backflow airflow to flow back to the heating air duct 12, and the backflow component 24 is close to the heating air outlet 122 of the heating air duct 12 relative to the backflow-preventing component 23.

Specifically, the reflow assembly 24 includes: a return flow guide part 241, a first return part 242, and a second return part 243; the backflow guiding portion 241 is configured to guide the backflow airflow to the first backflow portion 242, and the first backflow portion 242 is configured to turn back the backflow airflow guided to the first backflow portion 242 to the second backflow portion 243, so that the backflow airflow turned back to the second backflow portion 243 is discharged to the heating air duct 12 under the action of the second backflow portion 243.

In conclusion, the temperature control device of PCR amplificator that this application provided, heating drainage fan can guide the air current to flow through heating element to guide from heating channel's air outlet and flow through the mixing chamber and guide to wind bath cavity from wind bath air intake, form the heated air current to the air current heating through heating element, can control heating element's heating temperature through the heating temperature control spare, make heating temperature satisfy the required temperature of sample detection. When the actual temperature in the air bath chamber is higher than the temperature required by detection, the heating temperature generated by the heating assembly is controlled through the heating temperature control. When the difference between the actual temperature in the air bath cavity and the temperature required for detection is great, the refrigeration drainage fan can guide the air current to flow through the refrigeration assembly, and guide to the heating air duct from the air outlet of refrigeration passageway, form the refrigeration air current to the air current refrigeration through the refrigeration assembly, the mixing subassembly can carry out the mixing with heating air current and mixing air current, the air current of mixing satisfies the temperature required for detection, and flow into the air bath cavity through the air bath air intake. In each process of adjusting the air current temperature, the air current flowing into the air bath chamber can be discharged from the air outlet of the air bath, so that the heating air current is continuously supplemented into the air bath chamber, the circularly flowing heating air current is formed, and the uniformity of the heating temperature is ensured. The temperature required by the sample detection can be quickly obtained by heating the air flow, so that the detection efficiency is improved.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical personnel in the technical field of the utility model, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replace.

Claims

1. The temperature control device of the PCR amplification instrument is characterized by comprising: the heating mechanism, the refrigerating mechanism, the blending mechanism and the air bath chamber are arranged in the air bath chamber; the heating mechanism includes: the heating drainage fan, the heating air duct, the heating assembly and the heating temperature control part are arranged on the heating drainage fan; the refrigeration mechanism includes: the refrigeration drainage fan, the refrigeration air duct and the refrigeration assembly; the heating drainage fan and the heating assembly are both arranged inside the heating air channel; the heating temperature control is connected with the heating assembly, the heating assembly is used for generating heat, and the heating temperature control is used for controlling the temperature generated by the heating assembly; the air outlet of the refrigerating air duct is communicated with the heating air duct, and the refrigerating drainage fan and the refrigerating assembly are arranged inside the refrigerating air duct; the blending mechanism comprises: the mixing device comprises a mixing chamber and a mixing component, wherein the mixing chamber is provided with a mixing air inlet and a mixing air outlet, the mixing component is arranged inside the mixing chamber, and the mixing air inlet is communicated with the air outlet of the heating air duct; the air bath chamber is used for placing a sample to be amplified, and is also provided with a first visual window for observing the sample to be amplified; the air bath chamber is provided with an air bath air inlet and an air bath air outlet, and the air bath air inlet is communicated with the uniformly mixing air outlet; the heating drainage fan is used for guiding airflow to flow through the heating assembly, the refrigerating drainage fan is used for guiding airflow to flow through the refrigerating assembly, the blending assembly is used for blending airflow, and the air bath air outlet is used for exhausting airflow.

2. The temperature control device for a PCR amplifier as set forth in claim 1, further comprising: the air bath device comprises a square-shaped guide air duct, a water bath cavity and a water tank, wherein the square-shaped guide air duct is provided with an inner shell and an outer shell sleeved on the inner shell; the outer shell is provided with a flow guide air inlet, the inner shell is provided with a flow guide air outlet, the flow guide air inlet is communicated with the air outlet of the heating air duct, and the flow guide air outlet is communicated with the air bath air inlet of the air bath cavity; the square-shaped guide air duct is further provided with a second window opposite to the first visual window, and the second window penetrates through the inner shell and the outer shell.

3. The temperature control device for a PCR amplification apparatus as set forth in claim 1, wherein the cooling mechanism further comprises: the air outlet in refrigeration wind channel with the intercommunication phase in heating wind channel is relative to the position that heating element set up is close to in heating wind channel's air outlet, prevent that the subassembly that flows backwards is used for preventing that heating wind channel's backward flow air current from entering into the refrigeration wind channel.

4. The temperature control device for a PCR amplification apparatus according to claim 3, wherein the cooling mechanism further comprises: the backflow assembly is used for enabling backflow airflow to flow back to the heating air duct; the backflow component is close to the air outlet of the heating air duct relative to the backflow-preventing component.

5. The temperature control device of the PCR amplification apparatus of claim 3, wherein the backflow prevention assembly comprises: the backflow prevention guide part comprises a backflow prevention guide part, a first backflow prevention part and a second backflow prevention part; the backflow prevention flow guide part is used for guiding backflow airflow to the first backflow prevention part, the first backflow prevention part is used for enabling the backflow airflow guided to the first backflow prevention part to return back to the second backflow prevention part, and the backflow airflow is discharged from an air outlet of the refrigeration air duct.

6. The temperature control device of PCR amplification apparatus of claim 4, wherein the reflow module comprises: a backflow guide part, a first backflow part and a second backflow part; the backflow diversion part is used for diverting backflow airflow to the first backflow part, the first backflow part is used for enabling the backflow airflow diverted to the first backflow part to return to the second backflow part, and the second backflow part is used for enabling the backflow airflow to be discharged to the heating air duct.

7. The temperature control device of the PCR amplification instrument of claim 1, wherein the mixing component comprises: the blending driving unit is used for driving the blending rotating shaft to rotate so as to drive the blending fan blades to rotate; the axial lead of the uniformly mixing rotating shaft is coincided with the central axis of the heating air duct; the blending fan blade is provided with a plurality of hollow parts.

8. The temperature control device of the PCR amplification apparatus as claimed in claim 1, wherein a temperature measurement module and the cooling and drainage fan are further disposed inside the air bath chamber, and the temperature measurement module is connected to the heating temperature control unit, and is configured to detect temperature information in the air bath chamber and output the temperature information to the heating temperature control unit and the cooling and drainage fan.

9. The temperature control device of the PCR amplification instrument of claim 1, wherein the air outlet of the air bath is further provided with an air outlet fan of the air bath.

10. The temperature control device for a PCR amplification instrument of claim 1, wherein the air inlet of the air bath is arranged at the bottom end of the air bath chamber, and the air outlet of the air bath is arranged at the top end or the side surface of the air bath chamber.