CN217809337U

CN217809337U - Auxiliary device for separating peripheral blood mononuclear cells

Info

Publication number: CN217809337U
Application number: CN202221262688.7U
Authority: CN
Inventors: 张志鹏; 农海连
Original assignee: Guangzhou Binpeng Biotechnology Co ltd
Current assignee: Guangzhou Binpeng Biotechnology Co ltd
Priority date: 2022-05-24
Filing date: 2022-05-24
Publication date: 2022-11-15
Anticipated expiration: 2032-05-24

Abstract

The utility model relates to an auxiliary device for separation of peripheral blood mononuclear cell, including the lagging casing, the lagging casing fixed surface is provided with the supporting leg, the supporting leg fixed surface is provided with the base down, fixed surface is provided with the motor on the base, the terminal fixed motor main shaft that is provided with of motor, motor main shaft surface activity is provided with the lagging casing, motor main shaft fixed surface is provided with drive gear, the lagging casing upper surface is seted up flutedly, the inside activity of recess is provided with the slider, the terminal fixed centrifugal storehouse that is provided with of slider, the fixed collection box that is provided with in centrifugal storehouse bottom, the fixed slide rail that is provided with of centrifugal storehouse pipe wall, the inside activity of slide rail is provided with the apron, apron central point puts the activity and is provided with the motor main shaft, the utility model discloses, through the inside temperature in lagging casing control centrifugal storehouse for the separation effect is better, and is comparatively practical, is fit for extensively promoting and using.

Description

Auxiliary device for separating peripheral blood mononuclear cells

Technical Field

The utility model belongs to experiment auxiliary device field, specifically speaking relates to an auxiliary device that is used for separation of peripheral blood mononuclear cell.

Background

The whole organism of the monocyte is composed of a cell, the basic structure of the monocyte is a cell membrane, a cytoplasm and a cell nucleus, the monocyte has all life phenomena and can independently complete physiological functions which can be completed by organs, such as reproduction, growth, nutrition, respiration and the like, the monocyte is mainly distributed in fresh water, seawater and moist soil and can exchange materials and energy with the living environment of the monocyte, and the monocyte cannot generally live in a dry environment. The cell nucleus can be separated by cell nucleus division, so that experimenters can conveniently research the cell nucleus and know the genetic material of the cell.

The CN 205368329U searched discloses an auxiliary device for separating human peripheral blood mononuclear cells, which is a circular plastic cover plate, and cell separation holes are uniformly distributed on the plastic cover plate. Although the method can be used in the fields of cell therapy, clinical examination and scientific research tests, and is used for separation tests such as separation of lymphocytes and erythrocytes, the experimental difficulty can be greatly reduced, the operation time can be shortened, and the experiment can be more convenient, quicker and more efficient; the plastic cover plate is made of common plastic materials for cell culture, has no toxic or harmful effect on cells, and is safe and reliable.

However, the inventor finds that the technical scheme still has at least the following defects:

in actual operation, some mononuclear cells have relatively harsh conditions, such as a large influence on temperature, and the device does not have a temperature regulation device, which affects the separation effect of the device in the later period, and causes inaccurate later period experiments.

In view of this, the present invention is provided.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model adopts the following basic concept:

a auxiliary device for separation of peripheral blood mononuclear cell, including lagging casing, lagging casing fixed surface is provided with four supporting legs, four supporting leg fixed surface is provided with the base, fixed surface is provided with the motor on the base, the terminal fixed motor main shaft that is provided with of motor, motor main shaft surface activity is provided with lagging casing, motor main shaft fixed surface is provided with drive gear, the lagging casing upper surface is seted up flutedly, the inside activity of recess is provided with the slider, the terminal fixed centrifugal storehouse that is provided with of slider, the fixed collection box that is provided with in centrifugal storehouse bottom, the fixed slide rail that is provided with of centrifugal storehouse pipe wall, the inside activity of slide rail is provided with the apron, apron central point puts the activity and is provided with the motor main shaft, lagging casing upper surface mounting has the end cover, and the device has guaranteed that the temperature in the nuclear branch remains stable.

As a preferred embodiment of the utility model, the fixed two locating pieces that are provided with in motor side, two fixedly connected with set nut between locating piece and the base, after this machine starts, because the rotational speed of motor is fast, the motor at this moment is whole can take place to shake, connects it into a whole through set nut, can reduce the condition of shake and take place.

As a preferred embodiment of the utility model, the lagging casing fixed surface is provided with the feed liquor pipeline, the fixed feed liquor valve that is provided with in feed liquor pipeline central point puts, and the feed liquor pipeline can improve the inside temperature in heat preservation storehouse to the inside hot water that pours into of lagging casing into, and the feed liquor valve has played sealed effect.

As a preferred embodiment of the utility model, the lagging casing fixed surface is provided with the fluid-discharge tube, the fixed flowing back valve that is provided with of fluid-discharge tube central point puts, and the fluid-discharge tube can be discharged the inside refrigerated liquid of lagging casing for the lagging casing keeps dry, prevents that inside moisture from existing for a long time and causing the corruption, influences the lagging casing performance.

As a preferred embodiment of the present invention, the cover plate has a plurality of through holes formed on its surface, which plays a role of separating cytoplasm and nucleus under high-speed rotation.

As a preferred embodiment of the utility model, the fixed surface is provided with driven gear under the centrifugal storehouse, driven gear surface engagement is provided with drive gear, drives driven gear through drive gear and rotates for the centrifugal storehouse that links to each other with it can rotate at a high speed, reaches the purpose of separation.

As an optimized implementation manner of the utility model, the end cover is connected through cylindrical roller bearing with the motor main shaft, and cylindrical roller bearing makes the motor main shaft rotate more easily this moment to cylindrical roller bearing's roller is line contact or repaiies down line contact with the raceway, and radial bearing capacity is big, is applicable to and bears heavy load and impact load.

As the utility model discloses a preferred embodiment, the screw thread is seted up to the lagging casing upper surface, the screw thread has been seted up to the end cover internal surface, the lagging casing passes through the thread engagement installation with the end cover, makes the device's end cover easily dismantle through the screw thread, and the later stage of being convenient for collects materials such as cell nucleus.

Compared with the prior art, the utility model following beneficial effect has:

the utility model discloses, through feed liquor pipeline and fluid-discharge tube interact for the inside temperature of the device's lagging casing remains stable, can pass through the temperature in heat preservation storehouse after centrifugal storehouse begins to rotate, makes the device keep invariable temperature, makes the effect of separation better, has also improved separation efficiency.

The following describes in further detail embodiments of the present invention with reference to the accompanying drawings.

Drawings

In the drawings:

FIG. 1 is a cross-sectional view of an auxiliary device for peripheral blood mononuclear cell separation;

FIG. 2 is a schematic three-dimensional structure diagram of an auxiliary device for peripheral blood mononuclear cell separation;

FIG. 3 is a schematic diagram of the internal structure of the heat-insulating shell of the auxiliary device for separating peripheral blood mononuclear cells.

In the figure: 1. a heat-insulating shell; 2. supporting legs; 3. a base; 4. positioning blocks; 5. positioning a nut; 6. an electric motor; 7. a motor spindle; 8. a liquid discharge pipe; 9. a drain valve; 10. a drive gear; 11. a driven gear; 12. a collection box; 13. a cover plate; 14. an end cap; 15. a cylindrical roller bearing; 16. a liquid inlet valve; 17. a liquid inlet pipeline; 18. a groove; 19. a slider; 20. a slide rail; 21. and (4) centrifuging the bin.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and the following embodiments are used to illustrate the present invention.

As shown in fig. 1 to 3, an auxiliary device for separating peripheral blood mononuclear cells comprises an insulating shell 1, wherein four support legs 2 and four support legs 2 are fixedly arranged on the lower surface of the insulating shell 1, the base 3 is fixedly arranged on the lower surface of each support leg 2, the base 3 plays a role of a fixing device, a motor 6 is fixedly arranged on the upper surface of the base 3, a motor spindle 7 is fixedly arranged at the tail end of the motor 6, the motor spindle 7 is driven by the motor 6 to rotate, the insulating shell 1 is movably arranged on the surface of the motor spindle 7, a driving gear 10 is fixedly arranged on the surface of the motor spindle 7, the driving gear 10 also rotates, a groove 18 is formed in the upper surface of the insulating shell 1, a sliding block 19 is movably arranged in the groove, the sliding block 19 can slide in the groove 18, a centrifugal bin 21 is fixedly arranged at the tail end of the sliding block 19, a collecting box 12 is fixedly arranged at the bottom of the centrifugal bin 21, the collecting box 12 collects separated cell media, a sliding rail 20 is fixedly arranged on the pipe wall of the centrifugal bin 21, a cover plate 13 is movably arranged in the sliding rail 20, a movable cover plate 7 is arranged at the center of the sliding rail 13, and an end cover plate 14 is mounted on the upper surface of the insulating shell 1.

As shown in fig. 1 to 3, in a specific embodiment, two positioning blocks 4 are fixedly arranged on a side surface of the motor 6, a positioning nut 5 is fixedly connected between the two positioning blocks 4 and the base 3, the motor 6 is a high-speed motor and mainly plays a role of separation, when the motor starts to rotate at a high speed, the motor 6 starts to shake, and the positioning nut 5 is installed to integrate the motor of the device with the base 3, so that the shaking condition is reduced; 1 fixed surface of lagging casing is provided with

inlet fluid pipeline

17, 17 central point of inlet fluid pipeline puts the fixed inlet valve 16 that is provided with, 1 fixed surface of lagging casing is provided with fluid-

discharge tube

8, 8 central point of fluid-discharge tube puts the fixed drain valve 9 that is provided with, and inlet fluid pipeline 17 can be to the inside hot water that pours into of lagging casing into, improves the inside temperature in heat preservation storehouse, and inlet fluid valve 16 has played sealed effect, and fluid-discharge tube 8 can discharge the inside refrigerated liquid of lagging casing 1 for lagging casing 1 keeps dry, keeps on that inside moisture exists for a long time and causes the phenomenon of corruption, influences the heat retaining performance of lagging casing 1.

As shown in fig. 1 to 3, further, the cover plate 13 has a plurality of through holes on its surface, and mainly functions that after the centrifugal chamber 21 starts to centrifuge, the cytoplasm and cell nucleus in the cell are dispersed, and at this time, the cytoplasm flows down to the collection box through the cover plate 13, and the cell nucleus exists above the cover plate 13, so as to facilitate later processing, the lower surface of the centrifugal chamber 21 is fixedly provided with the driven gear 11, the driven gear 11 is engaged with the surface of the driven gear 11 and is provided with the driving gear 10, the driving gear 10 drives the driven gear 11 to start moving, so that the centrifugal chamber 21 can rotate at high speed, thereby avoiding the gear transmission inside the centrifugal chamber 2, reducing the debris generated by gear friction and falling into the centrifugal chamber 21, and affecting the separation effect of the device, the end cap 14 is connected with the motor spindle 7 through the cylindrical roller bearing 15, at this time, the cylindrical roller bearing 15 makes the motor spindle 7 rotate more easily, and the roller of the cylindrical roller bearing 15 is in line contact or off-line contact with the raceway, which the radial bearing capacity is large, and is suitable for bearing heavy load and impact load, the upper surface of the insulation shell 1 is provided with the end cap, and the insulation shell 14, and the device is easy to reduce the impact load caused by the impact on the separation effect of the separation of the cell nucleus caused by the end cap installation environment, and the separation device, and the separation effect of the end cap is easy to be removed.

The implementation principle of the auxiliary device for peripheral blood mononuclear cell separation of the embodiment is as follows: firstly, an operator opens an end cover 14, materials to be separated are placed on the surface of a cover plate 13, then high-temperature distilled water is injected into a liquid inlet pipeline 17, the temperature inside a centrifugal bin 21 is controlled, then a liquid inlet valve 16 is closed, then a motor 6 is started, the motor drives a motor spindle 7 to rotate, at the moment, a driving gear 10 on the motor spindle 7 also rotates, the driving gear 10 is meshed with a driven gear 11, the driven gear 11 drives the centrifugal bin 21 to rotate and to start centrifugal movement, at the moment, a sliding block 18 is connected to the upper surface of the centrifugal bin 21, the relative position of the centrifugal bin 21 is fixed, after the centrifugation is finished, cytoplasm and nuclei of cells are separated, due to the fluidity of the cytoplasm, at the moment, the cytoplasm flows into a collecting box 12 through a through hole in the surface of the cover plate 13, then the cover plate 13 is taken out to carry out post-stage treatment on the nuclei and cytoplasm, then a liquid discharge valve 8 is opened, moisture inside a heat-insulating shell 1 is discharged through a liquid discharge pipe 8, and the separation process of the device is finished.

Claims

1. A auxiliary device for separation of peripheral blood mononuclear cell, including lagging casing (1), its characterized in that, lagging casing (1) lower fixed surface is provided with four supporting legs (2), four supporting leg (2) lower fixed surface is provided with base (3), fixed surface is provided with motor (6) on base (3), motor (6) end is fixed and is provided with motor spindle (7), motor spindle (7) surface activity is provided with lagging casing (1), motor spindle (7) surface is provided with drive gear (10), lagging casing (1) upper surface is seted up flutedly (18), the inside activity of recess is provided with slider (19), slider (19) end is fixed and is provided with centrifugal storehouse (21), centrifugal storehouse (21) bottom is fixed and is provided with collection box (12), centrifugal storehouse (21) pipe wall is fixed and is provided with slide rail (20), the inside activity of slide rail (20) is provided with apron (13), apron (13) central point position activity is provided with motor spindle (7), surface mounting has end cover (14) on lagging casing (1).

2. The auxiliary device for peripheral blood mononuclear cell separation according to claim 1, wherein two positioning blocks (4) are fixedly arranged on the side of the motor (6), and a positioning nut (5) is fixedly connected between the two positioning blocks (4) and the base (3).

3. The auxiliary device for peripheral blood mononuclear cell separation according to claim 1, wherein a liquid inlet pipeline (17) is fixedly arranged on the surface of the heat preservation shell (1), and a liquid inlet valve (16) is fixedly arranged at the center of the liquid inlet pipeline (17).

4. The auxiliary device for peripheral blood mononuclear cell separation according to claim 3, wherein a drainage pipe (8) is fixedly arranged on the surface of the heat preservation shell (1), and a drainage valve (9) is fixedly arranged at the center of the drainage pipe (8).

5. The auxiliary device for peripheral blood mononuclear cell separation as claimed in claim 1, wherein the cover plate (13) has several through holes on its surface.

6. The auxiliary device for peripheral blood mononuclear cell separation as claimed in claim 1, wherein the centrifugal bin (21) is fixedly provided with a driven gear (11) on the lower surface, and the driven gear (11) is provided with a driving gear (10) on the surface in a meshing manner.

7. Auxiliary device for peripheral blood mononuclear cell separation according to claim 1, characterized in that the end cap (14) is connected to the motor spindle (7) by means of a cylindrical roller bearing (15).

8. The auxiliary device for peripheral blood mononuclear cell separation as claimed in claim 7, wherein the upper surface of the heat-insulating shell (1) is threaded, the inner surface of the end cap (14) is threaded, and the heat-insulating shell (1) and the end cap (14) are installed in a threaded engagement mode.