CN217781175U

CN217781175U - Carbon dioxide incubator for stem cell culture

Info

Publication number: CN217781175U
Application number: CN202222010595.1U
Authority: CN
Inventors: 董学平; 汪明; 陈爱强
Original assignee: Shanghai Ganyi Biomedical Technology Co ltd
Current assignee: Shanghai Ganyi Biomedical Technology Co ltd
Priority date: 2022-08-02
Filing date: 2022-08-02
Publication date: 2022-11-11
Anticipated expiration: 2032-08-02

Abstract

The utility model discloses a carbon dioxide incubator for stem cell culture, place case, reposition of redundant personnel admission section of thick bamboo, intake pipe, get thing door, culture medium culture heat transfer device, culture medium including the cultivation and place board and bleeder vent, the reposition of redundant personnel admission section of thick bamboo is located the cultivation and is placed on the case, the intake pipe link up with the reposition of redundant personnel admission section of thick bamboo, it gets the thing door to cultivate to be equipped with on placing the case, culture medium culture heat transfer device locates to cultivate and places on the case and run through the cultivation and place the case setting, the culture medium is placed the board and is located the cultivation and place the incasement, the culture medium is placed and is equipped with the bleeder vent on the board. The utility model belongs to the technical field of stem cell culture, specifically a carbon dioxide incubator is used in stem cell culture, the effectual stem cell culture for the present market in with carbon dioxide incubator uneven temperature that has solved, influence stem cell culture's problem, through setting up the effect that the backward flow formula heating methods realized the even heating.

Description

Carbon dioxide incubator for stem cell culture

Technical Field

The utility model belongs to the technical field of stem cell culture, specifically indicate a carbon dioxide incubator is used in stem cell culture.

Background

The carbon dioxide incubator is an advanced instrument for culturing cells, tissues and bacteria, is improved on the basis of common culture, and mainly can be added with carbon dioxide to meet the environment required by culturing microorganisms; stem cell culture is often performed on petri dishes, and also requires a suitably balanced temperature for stem cell culture.

At present to stem cell culture when heating be equipped with the heater in bottom half usually, then blow incubator upper portion with the hot-blast through the fan, realize the heating function, but the temperature difference in the box is bigger, and some local temperature is high, and some local temperature is low, influences stem cell's cultivation.

SUMMERY OF THE UTILITY MODEL

To the above situation, for overcoming prior art's defect, the utility model provides a carbon dioxide incubator is used in stem cell cultivation, the effectual stem cell cultivation for the current market carbon dioxide incubator in the temperature inhomogeneous that has solved influences stem cell cultivation's problem, has realized the effect of even heating through setting up backward flow formula heating mode.

The utility model adopts the following technical scheme: the utility model provides a carbon dioxide incubator for stem cell culture, including culture placement case, reposition of redundant personnel admission section of thick bamboo, intake pipe, get thing door, culture medium culture heat transfer device, culture medium place board and bleeder vent, reposition of redundant personnel admission section of thick bamboo is located on the culture placement case, the intake pipe is link up with reposition of redundant personnel admission section of thick bamboo, be equipped with on the culture placement case and get thing door, culture medium culture heat transfer device locates on the culture placement case and runs through culture placement case setting, culture medium place board locates in the culture placement case, be equipped with the bleeder vent on the culture medium place board, culture placement case carries out the dry cell culture, reposition of redundant personnel admission section of thick bamboo and intake pipe play the effect of injecting carbon dioxide, culture medium culture heat transfer device centre gripping and temperature transmission, culture medium place board and bleeder vent play the support and ventilative purpose, the heat conduction device is cultivateed to culture medium includes hot water filling tube, hot water discharge pipe, hot water back flow, culture medium centre gripping spring and culture medium holder, the hot water filling tube runs through the cultivation and places the setting of case lateral wall, the position that the hot water discharge pipe just located the hot water discharge pipe is less than the position of hot water filling tube, hot water back flow one end is located on the hot water filling tube, the hot water back flow other end is located on the hot water discharge pipe, culture medium centre gripping spring is located the cultivation and is placed on the incasement wall, be equipped with the culture medium holder on the culture medium centre gripping spring, hot water filling tube, hot water discharge pipe and hot water back flow through connection, hot water pass through hot water filling tube, hot water back flow and hot water discharge pipe after with the heat transfer for by culture medium centre gripping spring and culture medium holder centre gripping culture medium.

Preferably, get thing door and cultivate and place the case and be articulated the setting, the position of hot water discharge pipe is higher than the position of bleeder vent.

Further, culture medium centre gripping spring and culture medium holder are and deal with the setting, culture medium holder and culture medium place the board and be perpendicular setting, bleeder vent and hot water injection pipe are parallel arrangement, hot water injection pipe and hot water discharge pipe are parallel arrangement, culture medium centre gripping spring and hot water injection pipe are parallel arrangement

Furthermore, the hot water return pipe is arranged in a C-shaped pipeline, and the shunting air inlet cylinder is arranged in a U-shaped pipeline.

In addition, the hot water injection pipes are provided with a plurality of groups, the number of the hot water discharge pipes is equal to that of the hot water injection pipes, and the number of the hot water return pipes is the same as that of the hot water discharge pipes.

Adopt above-mentioned structure the utility model discloses the beneficial effect who gains as follows: the carbon dioxide incubator for stem cell culture that this scheme provided, the effectual stem cell culture that has solved for the present market carbon dioxide incubator in the temperature inhomogeneous, influence stem cell culture's problem, realized the effect of even heating through setting up backward flow formula heating methods.

Drawings

FIG. 1 is a schematic view of the overall structure of a carbon dioxide incubator for stem cell culture according to the present invention;

FIG. 2 is a sectional view of a carbon dioxide incubator for stem cell culture according to the present invention;

FIG. 3 is a cross-sectional view of another perspective of the carbon dioxide incubator for stem cell culture according to the present invention.

Wherein, 1, cultivate and place the case, 2, reposition of redundant personnel air inlet section of thick bamboo, 3, intake pipe, 4, get the thing door, 5, culture medium cultivation heat-transfer device, 6, culture medium place the board, 7, bleeder vent, 8, hot water injection pipe, 9, hot water discharge pipe, 10, hot water back flow, 11, culture medium centre gripping spring, 12, culture medium holder.

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the principles of the invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments; based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in figure 1, figure 2 and figure 3, the carbon dioxide incubator for stem cell culture comprises an incubator 1, a split-flow air inlet pipe 2, an air inlet pipe 3, a fetching door 4 and a culture medium culture heat conducting device 5, culture medium placing plate 6 and bleeder vent 7, reposition of redundant personnel admission cylinder 2 is located and cultivates on placing case 1, intake pipe 3 link up with reposition of redundant personnel admission cylinder 2, cultivate and be equipped with on placing case 1 and get thing door 4, culture medium culture heat transfer device 5 locates and cultivates and place case 1 and set up through the cultivation, culture medium placing plate 6 locates and cultivates and place the case 1 in, culture medium placing plate 6 is equipped with bleeder vent 7, cultivate and place case 1 and carry out the dry cell cultivation, reposition of redundant personnel admission cylinder 2 and intake pipe 3 play the effect of pouring into carbon dioxide into, culture medium culture heat transfer device 5 is to culture medium centre gripping and temperature transfer, culture medium placing plate 6 and bleeder vent 7 play the purpose of support and breathing freely, culture medium culture heat transfer device 5 includes hot water injection pipe 8, hot water discharge pipe 9, hot water discharge pipe 10, hot water centre gripping spring 11 and culture medium holder 12, hot water injection pipe 8 runs through and cultivates and places case 1 lateral wall setting, hot water discharge pipe 9 runs through the position of placing case 1 lateral wall and is less than hot water injection pipe 8, hot water injection pipe 10 locates hot water reflux pipe 10 on the other end of hot water injection pipe 8, hot water injection spring holder 10 and culture medium holder 12, hot water injection pipe 10 is located on the hot water injection pipe, culture medium holding spring 8, culture medium inner wall, culture medium holder is connected with culture medium holder 12, hot water injection pipe, culture medium discharge pipe 10, hot water injection spring 11, culture medium holder is located on culture medium holding device, culture medium holder, hot water injection pipe 12, hot water injection pipe 10.

As shown in FIG. 1, FIG. 2 and FIG. 3, the fetching door 4 and the culture placing box 1 are hinged, and the position of the hot water discharging pipe 9 is higher than the position of the ventilating hole 7.

As shown in FIG. 1, FIG. 2 and FIG. 3, the medium holding spring 11 and the medium holding member 12 are disposed, the medium holding member 12 and the medium placing plate 6 are disposed vertically, the air holes 7 and the hot water injection pipe 8 are disposed in parallel, the hot water injection pipe 8 and the hot water discharge pipe 9 are disposed in parallel, and the medium holding spring 11 and the hot water injection pipe 8 are disposed in parallel

As shown in fig. 1, 2 and 3, the hot water return pipe 10 is disposed in a C-shaped pipeline, and the bypass air intake cylinder 2 is disposed in a U-shaped pipeline.

As shown in fig. 1, 2 and 3, the hot water injection pipes 8 are provided in a plurality of groups, the number of the hot water discharge pipes 9 is equal to the number of the hot water injection pipes 8, and the number of the hot water return pipes 10 is the same as the number of the hot water discharge pipes 9.

During the specific use, the user opens and gets thing door 4 and places the stem cell culture medium on culture medium places board 6, and through culture medium clamping spring 11 and culture medium holder 12 with the lateral wall centre gripping of culture medium and fix, the user afterwards switches on hot water inlet tube and hot water injection pipe 8, and switch on the outlet pipe to the water that discharges in the hot water discharge pipe 9, inject carbon dioxide inwards through intake pipe 3 and reposition of redundant personnel air inlet pipe 2 during the cultivation afterwards, and cooperate the suitable water of temperature to pour into towards the cultivation and place the case 1 into, the water that the temperature is known at this moment goes into the cultivation and places the case 1 through hot water injection pipe 8, and flow back through hot water back flow 10 and place case 1 from the discharge of hot water discharge pipe 9 discharge, carry out the temperature transfer to the culture medium that culture medium clamping spring 11 and culture medium holder 12 held in the hot-water circulation in-process, provide the required temperature of culture medium cultivation, realize the even effect of heat conduction, above is the use flow of whole carbon dioxide incubator for the stem cell culture.

It should be noted that, in this document, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

The present invention and the embodiments thereof have been described above, but the description is not limited thereto, and the embodiment shown in the drawings is only one of the embodiments of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should understand that they should not be limited to the embodiments described above, and that they can design the similar structure and embodiments without departing from the spirit of the invention.

Claims

1. The utility model provides a carbon dioxide incubator is used in stem cell culture which characterized in that: place case, reposition of redundant personnel air inlet cylinder, intake pipe, get thing door, culture medium and cultivate heat transfer device, culture medium and place board and bleeder vent including cultivateing, reposition of redundant personnel air inlet cylinder locates to cultivate and places on the case, the intake pipe link up with reposition of redundant personnel air inlet cylinder, the cultivation is placed and is equipped with on the case and gets the thing door, culture medium cultivates heat transfer device and locates to cultivate and place on the case and run through the cultivation and place the case setting, the culture medium is placed the board and is located to cultivate and place the incasement, be equipped with the bleeder vent on the board is placed to the culture medium, culture medium cultivates heat transfer device and includes hot water injection pipe, hot water discharge pipe, hot water back flow, culture medium centre gripping spring and culture medium holder, the hot water injection pipe is equipped with culture medium holder on the culture medium centre gripping spring, hot water injection pipe, hot water discharge pipe and hot water back flow through connection.

2. The carbon dioxide incubator according to claim 1, wherein: get the thing door and cultivate and place the case and be articulated setting, the position of hot water discharge pipe is higher than the position of bleeder vent.

3. The carbon dioxide incubator according to claim 2, wherein: culture medium centre gripping spring and culture medium holder are the processing setting, culture medium holder and culture medium place the board and be perpendicular setting, bleeder vent and hot water injection pipe are parallel arrangement, hot water injection pipe and hot water discharge pipe are parallel arrangement, culture medium centre gripping spring and hot water injection pipe are parallel arrangement.

4. The carbon dioxide incubator according to claim 3, wherein: the hot water return pipe is arranged in a C-shaped pipeline, and the shunting air inlet cylinder is arranged in a U-shaped pipeline.

5. The carbon dioxide incubator according to claim 4, wherein: the hot water injection pipes are provided with a plurality of groups, the number of the hot water discharge pipes is equal to that of the hot water injection pipes, and the number of the hot water return pipes is equal to that of the hot water discharge pipes.